CN218089550U

CN218089550U - A cultivate equipment for cultivateing methane-oxidizing bacteria on a large scale

Info

Publication number: CN218089550U
Application number: CN202222521202.3U
Authority: CN
Inventors: 包智华; 白涵盖
Original assignee: Inner Mongolia University
Current assignee: Inner Mongolia University
Priority date: 2022-09-23
Filing date: 2022-09-23
Publication date: 2022-12-20
Anticipated expiration: 2032-09-23

Abstract

The utility model discloses a culture device for large-scale culture of methane-oxidizing bacteria, which comprises a reaction kettle and a sealing cover plate; the reaction kettle is of a double-layer structure and comprises an inner container and an outer container, the top ends of the inner container and the outer container are of an integral structure, the bottom end of the inner container is of a double-layer separation structure, a liquid outlet pipeline is arranged in the center of the bottom end of the inner container, the top end of the liquid outlet pipeline is of a conical structure, the bottom end of the liquid outlet pipeline is vertical, the bottom end of the liquid outlet pipeline penetrates through the center of the bottom end of the outer container, and the interior of the liquid outlet pipeline is communicated with the interior of the inner container and the exterior of the bottom end of the reaction kettle; the utility model discloses large-scale methane oxidation fungus culture can be carried out, its inside special-shaped stirring structure that adopts can increase methane dissolution efficiency to can keep warm or heat internal reaction environment through adding if heating liquid between double-deck reation kettle, the bottom adopts and to block the liquid outlet pipe way that can block and communicate and can realize sealed or drawing liquid, and the sealed leakproofness of equipment also can be guaranteed with adjustable exchange mouth to the apron of top seal, promotes the efficiency and the safety degree of reaction.

Description

A cultivate equipment for cultivateing methane-oxidizing bacteria on a large scale

Technical Field

The utility model relates to a methane-oxidizing bacteria cultivates technical field, specifically is a cultivation equipment for cultivateing methane-oxidizing bacteria on a large scale.

Background

The methane-oxidizing bacteria are microorganisms taking methane as energy and carbon sources, and have wide utilization potential in production practice, such as conversion of organic matters, degradation of environmental pollutants, production of valuable industrial products, reduction of emission of soil methane and the like, so that the greenhouse gas effect is reduced; the methane-oxidizing bacteria have the characteristics of slow growth, difficult culture, environmental sensitivity and the like. Simultaneously, compared with dissolved oxygen, the solubility of methane is lower, and the growth difficulty of methane-oxidizing bacteria is increased. Therefore, researchers have conducted much research into the design of reactors.

The methane-oxidizing bacteria can be classified into aerobic methane-oxidizing bacteria and anaerobic methane-oxidizing bacteria, and the anaerobic methane-oxidizing bacteria can be subdivided into sulfate type anaerobic methanation, nitrate type or nitrite type anaerobic methanation and ferro-manganese type anaerobic methane oxidation according to different types of electron acceptors. Recently, it has been discovered that methane-oxidizing bacteria can oxidize methane into carbon dioxide and water by using an electrode as an electron acceptor, and the generated electrons are transferred to the electrode, and the electrons can be transferred to a final electron acceptor through an external circuit, so as to generate electricity, organic acid, hydrogen and the like. The control of the methane-oxidizing bacteria culture, enrichment and valuable products can be realized through the control of the electric potential, and the method has very important application significance.

In the prior art, the following technical problems exist in the actual operation process of the reactor: (1) The existing methane oxidizing bacteria culture adopts a small-sized reaction kettle, culture liquid and methane oxidizing bacteria are placed in the reaction kettle, and stirring work is not needed, but under the conditions of large size and large amount, the methane solubility in the culture liquid is low, the requirements on environmental conditions are strict, the methane dissolving efficiency of the traditional culture mode is low (2) the existing equipment has poor sealing measures, and particularly openings, valves and other positions cause slow growth of the methane oxidizing bacteria.

Therefore, a culture apparatus for large-scale culture of methane-oxidizing bacteria is proposed.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a cultivate equipment for cultivateing methane-oxidizing bacteria on a large scale to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above purpose, the utility model provides a following technical scheme: a culture equipment for large-scale culture of methane-oxidizing bacteria comprises a reaction kettle and a sealing cover plate; the reaction kettle is of a double-layer structure and comprises an inner container and an outer container, the top ends of the inner container and the outer container are of an integral structure, the bottom end of the inner container is of a double-layer separation structure, a liquid outlet pipeline is arranged at the center of the bottom end of the inner container, the top end of the liquid outlet pipeline is of a conical structure, the bottom end of the liquid outlet pipeline is vertical and penetrates through the center of the bottom end of the outer container, the interior of the liquid outlet pipeline is communicated with the interior of the inner container and the exterior of the bottom end of the reaction kettle, a heating liquid outlet pipeline is arranged at the bottom end of the outer container outside the liquid outlet pipeline, a valve is arranged on the heating liquid outlet pipeline, the heating liquid outlet pipeline is fixedly arranged at the bottom of the outer container and is communicated with the interior of the outer container, a liquid extraction port is fixedly arranged on the liquid outlet pipeline at one side opposite to the heating liquid outlet pipeline and is communicated with the interior of the liquid outlet pipeline, and a liquid extraction pump is arranged outside the liquid extraction port, the inner of liquid outlet pipe is provided with a guide blocking device, the top end of the guide blocking device is extruded and attached to the conical inner wall of the top end of the liquid outlet pipe, the bottom end of the guide blocking device is fixedly mounted at the outer bottom end of the liquid outlet pipe, the sealing cover plate is located at the top end of the reaction kettle and fixedly connected with the top end face of the reaction kettle through a connecting piece, a stirring device is arranged in the middle of the sealing cover plate, the lower end of the stirring device is located in the reaction kettle, a heating liquid inlet pipe is arranged at the top end of the sealing cover plate and the reaction kettle in an inserting mode, the inner end of the heating liquid inlet pipe is located between the inner container and the outer container, an exchange port is further formed in the sealing cover plate, and the inner portion of the exchange port is communicated with the inner container.

Preferably, the guide blocking device comprises a sealing plug and a pushing assembly, the sealing plug is of a conical structure, the outer wall of the upper end of the sealing plug is extruded and attached to the conical inner wall of the top end of the liquid outlet pipeline, the pushing assembly is located inside the bottom end of the liquid outlet pipeline, the telescopic end of the pushing assembly is fixedly connected with the bottom end of the sealing plug, an installation sleeve is sleeved outside the pushing assembly, the installation sleeve is attached to the bottom end of the liquid outlet pipeline and fixedly connected with the bottom end of the liquid outlet pipeline through a fixing bolt 0, and after the pushing assembly is collected, the sealing plug is located below the horizontal plane of the liquid suction port.

Preferably, a rubber layer is arranged at the joint of the sealing cover plate and the top end of the reaction kettle.

Preferably, the stirring device comprises a motor and a driving shaft, the motor is fixedly installed in the middle of the sealing cover plate, the driving shaft is located inside the inner container, a stirring frame is arranged at the lower end of the driving shaft, and the stirring frame is of a double-layer annular structure and is arranged oppositely.

Preferably, the end of the inner side of the sealing cover plate, which is provided with the reaction kettle, is provided with three sensors, and the three sensors respectively maintain an oxygen concentration sensor, a methane concentration sensor and a temperature sensor.

Preferably, the upper side of the sealing cover plate is provided with a data panel, the data panel is electrically connected with the inductor, and the data panel displays induction data in real time and records the data at any time.

Preferably, an annular pressing plate is arranged on the outer wall of the heating liquid inlet pipeline, a pressing plate groove is formed in a sealing cover plate inserted into the heating liquid inlet pipeline, the annular pressing plate is located in the pressing plate groove, a sealing deformation pad is sleeved between the bottom end of the annular pressing plate and the bottom end of the pressing plate groove and is in extrusion contact with the bottom ends of the annular pressing plate and the pressing plate groove, and the inserted part of the heating liquid inlet pipeline and the sealing cover plate is in threaded connection.

Preferably, the outer side of the heating liquid inlet pipeline is provided with threads

Compared with the prior art, the beneficial effects of the utility model are that:

(1) The utility model discloses can carry out large-scale methane-oxidizing bacteria and cultivate, its inside special-shaped stirring structure that adopts can increase methane dissolution efficiency to can keep warm or heat internal reaction environment through heating liquid between double-deck reation kettle, and then to under large-scale, the many circumstances of volume, for traditional cultivation mode, make methane dissolution efficiency better.

(2) The bottom end of the reaction kettle is provided with a liquid outlet pipeline which can be blocked and communicated, so that sealing or liquid extraction can be realized, the sealing performance of the equipment can be ensured by the cover plate with the sealed top end and the adjustable exchange port, and the sealing performance and the safety degree of the reaction process are improved.

(3) The sensor of design can discern oxygen concentration, methane concentration and temperature respectively in the sealed apron, and sensor and data panel electric connection can show data real-time and have the advantage of record data moreover, can also copy daily data if necessary.

Drawings

FIG. 1 is a schematic view of the main structure of a culture apparatus of the present invention in example 1;

FIG. 2 is a schematic view of the bottom end structure of example 1 of the culture apparatus of the present invention;

FIG. 3 is a schematic sectional view of the culture apparatus of example 1 according to the present invention;

FIG. 4 is an enlarged view of FIG. 3 at A;

FIG. 5 is an enlarged view of FIG. 3 at B;

fig. 6 is a schematic sectional structure view of a liquid inlet installation method of embodiment 2.

In the figure: 1. reaction kettle, 11, inner container, 12, outer container, 13, liquid outlet pipeline, 14, sealing plug, 15, pushing component, 16, heating liquid outlet pipeline, 161, valve, 17, liquid drawing port, 18, liquid drawing pump, 19, mounting sleeve, 20, fixing bolt, 21, rubber layer, 22, connecting piece, 23, heating liquid inlet pipeline, 231, annular pressing plate, 232, sealing deformation pad, 24, exchange port, 25, pressing plate groove, 2, sealing cover plate, 3, motor, 31, driving shaft, 32, stirring frame, 4, sensor, 5, data panel.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Example 1: referring to fig. 1, the present invention provides a technical solution: a culture equipment for large-scale culture of methane-oxidizing bacteria comprises a reaction kettle 1 and a sealing cover plate 2.

Referring to fig. 2 and 3, in order to solve the problem that the methane dissolution efficiency is low due to low methane solubility and strict requirements on environmental conditions in the culture solution, a reaction kettle 1 is formed in a double-layer structure and comprises an inner container 11 and an outer container 12, the top ends of the inner container 11 and the outer container 12 are of an integrated structure, the bottom end of the inner container 11 and the bottom end of the outer container 12 are of a double-layer separation structure, a heating liquid inlet pipeline 23 is arranged at the joint of the sealing cover plate 2 and the top end of the reaction kettle 1 in an inserting manner, the inner end of the heating liquid inlet pipeline 23 is located between the inner container 11 and the outer container 12, heating liquid can be added between the inner container 11 and the outer container 12 through the heating liquid inlet pipeline 23, so that heating and heat preservation can be realized through the outer container 12, a heating liquid outlet pipeline 16 is arranged at the bottom end of the outer container 12 outside a liquid outlet pipeline 13, the heating liquid outlet pipeline 16 is fixedly installed at the bottom of the outer container 12 and is communicated with the interior of the outer container 12, and the heating liquid is discharged through the heating liquid outlet pipeline 16; a valve 161 is further provided in the heating fluid outlet line 16 to facilitate control of the discharge of the heating fluid.

In order to improve the methane dissolving efficiency, a stirring device is arranged in the middle of the sealing cover plate 2, the inner container 11 is stirred by the stirring device, the internal reaction dissolving efficiency can be increased, the lower end of the stirring device is positioned in the reaction kettle 1, the middle of the inner container 11, the stirring device comprises a motor 3 and a driving shaft 31, the motor 3 adopts a small alternating current asynchronous motor, the motor 3 is fixedly arranged in the middle of the sealing cover plate 2, the driving shaft 31 is positioned in the inner container 11, the lower end of the driving shaft 31 is provided with a stirring frame 32, the inner container 11 is fully stirred by considering the inner area of the inner container 11, the stirring frame 32 cannot excessively occupy the inner volume of the inner container 11, the stirring frame 32 is designed into a double-layer annular structure and is arranged mutually, the double-layer annular structure can stir in a large opposite direction and in a small stirring area (as shown in fig. 3), the stirring can be used for stirring the edge area of the inner container 11, the central part of the inner container 11 can also be stirred by the design, and the stirring frame 32 cannot excessively occupy the area of the inner container 11, so as to further play a role in improving the internal reaction dissolving efficiency.

In order to facilitate the discharge of the reaction liquid inside the inner container 11, a liquid outlet pipe 13 is arranged at the center of the bottom end of the inner container 11, the inside of the liquid outlet pipe 13 is communicated with the inside of the inner container 11 and the outside of the bottom end of the reaction kettle 1, a liquid pumping port 17 is fixedly arranged on one side of the liquid outlet pipe 13 opposite to the heating liquid outlet pipe 16 and is communicated with the inside of the liquid outlet pipe 13, and a liquid pumping pump 18 is arranged outside the liquid pumping port 17 to facilitate the discharge of the reaction liquid inside the reaction kettle 1.

Aiming at the problem of poor sealing performance of a liquid outlet of the existing equipment, the structural shape of a liquid outlet pipeline 13 communicated with an inner container 11 is modified, and a guide blocking device matched with the structural shape is designed; the top end of the liquid outlet pipeline 13 is in a conical structure, the bottom end of the liquid outlet pipeline is vertical, the bottom end of the liquid outlet pipeline passes through the center of the bottom end of the outer container 12, a guide blocking device is arranged inside the liquid outlet pipeline 13, the top end of the guide blocking device is in extrusion fit with the conical inner wall at the top end of the liquid outlet pipeline 13, the bottom end of the guide blocking device is fixedly installed outside the bottom end of the liquid outlet pipeline 13, the guide blocking device comprises a sealing plug 14 and a pushing assembly 15, the pushing assembly 15 adopts a straight rod type small electric pushing rod, the sealing plug 14 is in a conical structure, the outer wall of the upper end of the sealing plug is in extrusion fit with the conical inner wall at the top end of the liquid outlet pipeline 13, sealing performance can be guaranteed during fitting, the pushing assembly 15 is located inside the bottom end of the liquid outlet pipeline 13, a telescopic end of the pushing assembly 15 is fixedly connected with the bottom end of the sealing plug 14, in order to fix the pushing assembly 15 on the liquid outlet pipeline 13, a mounting sleeve 19 is sleeved outside the pushing assembly 15, the mounting sleeve 19 is fixedly connected with the bottom end of the liquid outlet pipeline 13 through a fixing bolt 20 (as shown in fig. 5), after the pushing assembly 15 is retracted, the sealing plug 14 is located below a liquid outlet 17, and the liquid outlet is convenient for drawing out the liquid level of the liquid outlet 17.

Sealed apron 2 is located reation kettle 1's top to through connecting piece 22 and reation kettle 1's top end fixed connection, connecting piece 22 can adopt the screw, can only better seal with reation kettle 1 for sealed apron 2, sets up rubber layer 21 (as figure 4) in sealed apron 2 and reation kettle 1's top laminating department, plays sealed effect of keeping apart.

In order to facilitate air exchange in the inner container 11 and to take certain influence on sealing caused by the arrangement of a plurality of air exchange ports into consideration, the sealing cover plate 2 is provided with an exchange port 24 with multiple ports integrated, and the inside of the exchange port 24 is communicated with the inside of the inner container 11; it can the evacuation and can take a breath in the later stage, has also avoided the condition of punching more on sealed apron 2.

In order to achieve better man-machine interaction of the equipment, one end of the inner side of the sealing cover plate 2, which is provided with the reaction kettle 1, is provided with three sensors 4, and the three sensors 4 are respectively an existing oxygen concentration sensor, a methane concentration sensor and a temperature sensor. The upper side of the sealing cover plate 2 is provided with a data panel 5, the data panel 5 is electrically connected with the inductor 4, and the data panel 5 displays induction data in real time and records the data at any time; the sensor 4 of design can discern oxygen concentration, methane concentration and temperature respectively in the sealed apron 2, and sensor 4 and data panel 5 electric connection moreover can show data in real time and have the advantage of record data, can also copy daily data if required.

The working principle is as follows: after reaction liquid is added into the inner container 11, heating liquid is added between the inner container 11 and the outer container 12 through the heating liquid inlet pipeline 23, the balance of internal reaction temperature is guaranteed, and the stirring frame 32 can be driven by the motor 3 to rotate after reaction to improve the dissolving efficiency of the internal reaction. After the reaction is finished, the sealing plug 14 is driven to be drawn out through the pushing assembly 15 at the bottom end, the liquid drawing port 17 is communicated with the inside of the inner container 11, reaction liquid in the inner container 11 is convenient to draw out, the sealing plug 14 is reset and then is extruded and attached to the inner wall of the top end of the liquid outlet pipeline 13 to guarantee sealing, heating liquid can be discharged through the heating liquid outlet pipeline 16, the sensor 4 senses the oxygen concentration, the methane concentration and the temperature in the inner part in real time, and the oxygen concentration, the methane concentration and the temperature are displayed on the data panel 5.

Example 2: as shown in fig. 5, in order to enable the heating fluid inlet pipeline 23 to be better sealed with the sealing cover plate 2, an annular pressing plate 231 is arranged on the outer wall of the heating fluid inlet pipeline 23, a pressing plate groove 25 is arranged inside the sealing cover plate 2 where the heating fluid inlet pipeline 23 is inserted, the annular pressing plate 231 is located in the pressing plate groove 25, a sealing deformation pad 232 is sleeved between the bottom end of the annular pressing plate 231 and the bottom end of the pressing plate groove 25, the sealing deformation pad 232 is in pressing contact with the bottom ends of the annular pressing plate 231 and the pressing plate groove 25, threads are arranged on the outer side of the heating fluid inlet pipeline 23, and the insertion part of the heating fluid inlet pipeline 23 and the sealing cover plate 2 is in threaded connection. The gap between the outside of the heating liquid inlet pipe 23 and the sealing cover plate 2 is sealed to provide the sealing property inside the inner container 11, and the rest of the characteristics are the same as those of embodiment 1.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A culture equipment for large-scale culture of methane-oxidizing bacteria comprises a reaction kettle (1) and a sealing cover plate (2); the method is characterized in that: the reaction kettle (1) is of a double-layer structure and comprises an inner container (11) and an outer container (12), the top ends of the inner container (11) and the outer container (12) are of an integrated structure, and the bottom end of the inner container and the bottom end of the outer container are of a double-layer separation structure; a liquid outlet pipeline (13) is arranged at the center of the bottom end of the inner container (11), the top end of the liquid outlet pipeline (13) is of a conical structure, the bottom end of the liquid outlet pipeline is vertical, the bottom end of the liquid outlet pipeline penetrates through the center of the bottom end of the outer container (12), the interior of the liquid outlet pipeline (13) is communicated with the interior of the inner container (11) and the exterior of the bottom end of the reaction kettle (1), a heating liquid outlet pipeline (16) is arranged at the bottom end of the outer container (12) outside the liquid outlet pipeline (13), a valve (161) is arranged on the heating liquid outlet pipeline (16), and the heating liquid outlet pipeline (16) is fixedly arranged at the bottom of the outer container (12) and is communicated with the interior of the outer container (12); a heating liquid inlet pipeline (23) is inserted into the joint of the sealing cover plate (2) and the top end of the reaction kettle (1), and the inner end of the heating liquid inlet pipeline (23) is positioned between the inner container (11) and the outer container (12);

a liquid pumping port (17) is fixedly arranged on the liquid outlet pipeline (13) at the side opposite to the heating liquid outlet pipeline (16) and is communicated with the inside of the liquid outlet pipeline (13), a liquid pumping pump (18) is arranged outside the liquid pumping port (17), a guiding and blocking device is arranged inside the liquid outlet pipeline (13), the top end of the guiding and blocking device is in extrusion fit with the conical inner wall at the top end of the liquid outlet pipeline (13), and the bottom end of the guiding and blocking device is fixedly arranged outside the bottom end of the liquid outlet pipeline (13);

the sealing cover plate (2) is positioned at the top end of the reaction kettle (1) and is fixedly connected with the top end face of the reaction kettle (1) through a connecting piece (22), a stirring device is arranged in the middle of the sealing cover plate (2), and the lower end of the stirring device is positioned in the reaction kettle (1);

the sealing cover plate (2) is also provided with an exchange port (24), and the inside of the exchange port (24) is communicated with the inside of the inner container (11).

2. The culture apparatus for mass-culturing methanotrophs according to claim 1, wherein: the guide blocking device comprises a sealing plug (14) and a pushing assembly (15), the sealing plug (14) is of a conical structure, the outer wall of the upper end of the sealing plug is extruded and attached to the conical inner wall of the top end of the liquid outlet pipeline (13), the pushing assembly (15) is located inside the bottom end of the liquid outlet pipeline (13), the telescopic end of the pushing assembly is fixedly connected with the bottom end of the sealing plug (14), an installation sleeve (19) is sleeved outside the pushing assembly (15), the installation sleeve (19) is attached to the bottom end of the liquid outlet pipeline (13), the bottom end of the liquid outlet pipeline (13) is fixedly connected with the bottom end of the fixing bolt (20), and after the pushing assembly (15) is collected, the sealing plug (14) is located below the horizontal plane of the liquid suction port (17).

3. The culture apparatus for mass-culturing methanotrophs according to claim 1, wherein: and a rubber layer (21) is arranged at the joint of the sealing cover plate (2) and the top end of the reaction kettle (1).

4. The culture apparatus for mass-culturing methanotrophs according to claim 1, wherein: agitating unit includes motor (3) and drive shaft (31), and motor (3) fixed mounting is in the centre of sealed apron (2) to its drive shaft (31) are located the inside of inner bag (11), the lower extreme of drive shaft (31) is provided with stirring frame (32), and stirring frame (32) are double-deck cyclic structure, and mutual subtend setting.

5. The culture apparatus for mass-culturing methanotrophs according to claim 1, wherein: one of the inner sides of the sealing cover plates (2) provided with the reaction kettle (1) is provided with three sensors (4), and the sensors (4) respectively maintain an oxygen concentration sensor, a methane concentration sensor and a temperature sensor.

6. The culture apparatus for large-scale culture of methanotrophs according to claim 1, wherein: sealed apron (2) upside is equipped with data panel (5), data panel (5) and inductor (4) electric connection, data panel (5) show response data in real time and record data at any time.

7. The culture apparatus for mass-culturing methanotrophs according to claim 1, wherein: the heating device is characterized in that an annular pressing plate (231) is arranged on the outer wall of the heating liquid inlet pipeline (23), a pressing plate groove (25) is formed in a sealing cover plate (2) where the heating liquid inlet pipeline (23) penetrates, the annular pressing plate (231) is located in the pressing plate groove (25), a sealing deformation pad (232) is sleeved between the bottom end of the annular pressing plate (231) and the bottom end of the pressing plate groove (25), the sealing deformation pad (232) is in extrusion contact with the bottom end of the annular pressing plate (231) and the bottom end of the pressing plate groove (25), and the penetrating position of the heating liquid inlet pipeline (23) and the sealing cover plate (2) is in threaded connection.

8. The culture apparatus for mass-culturing methanotrophs according to claim 1, wherein: and threads are arranged on the outer side of the heating liquid inlet pipeline (23).