CN218068436U - Microscope imaging focusing system and microscope - Google Patents

Abstract

The utility model belongs to the technical field of microscopes, and discloses a microscope imaging focal locking system and a microscope, which comprises a light source, a first lens, a second lens, a third lens, a detector and an objective lens displacement controller; the light source is used for generating incident light, the first lens, the second lens and the third lens are sequentially arranged on a propagation path of a reflected light beam, and the reflected light beam is formed by reflecting the incident light beam on the upper surface of the glass slide; the first lens and the second lens form a beam expanding lens group, the beam expanding lens group is used for amplifying offset, and the third lens is used for converging a reflected light beam to a detector arranged at a first angle. Has the beneficial effects that: the utility model discloses a lock burnt system of incident light through objective incident not, the dependence and the influence to the micro-imaging system can be ignored when locking burnt; the coke locking system has the advantages of small number of devices, simplified mechanism, reduced processing and debugging difficulty and easier realization of a modular system.

Description

Microscope imaging focusing system and microscope

Technical Field

The utility model relates to a microscope technical field especially relates to a microscope imaging lock burnt system and microscope.

Background

Due to the influence of vibration and temperature in the environment, when microscopic imaging is carried out, a sample inevitably suffers axial deviation under the influence of various factors in the environment, so that the surface of the sample is defocused, and under an objective lens with larger multiplying power, the influence caused by the deviation of the focal surface is larger. There is therefore a need to add a focal lock system to the microscope.

The existing focus locking system needs to amplify a detection signal and a feedback signal by using a dichroic mirror and an objective lens, and the focus locking system is required to have higher coaxiality, so that the structure of the focus locking system is complex and the assembly and adjustment are difficult.

SUMMERY OF THE UTILITY MODEL

The utility model discloses an aim at: the focus locking system independent of the microscope system is provided, the complexity of the focus locking system is reduced, and the focus locking system is more convenient to install and adjust.

In order to achieve the above object, the present invention provides a micro-imaging focusing system, which comprises a light source, a first lens, a second lens, a third lens, a detector and an objective lens displacement controller; the light source is used for generating incident light, the first lens, the second lens and the third lens are sequentially arranged on a propagation path of a reflected light beam, and the reflected light beam is formed by reflecting the incident light beam on the upper surface of the glass slide; the beam expander set is composed of the first lens and the second lens and used for amplifying offset, the third lens is used for converging a reflected light beam to a detector arranged at a first angle, the detector is used for acquiring the position of a light spot, and the detector is connected with the objective lens displacement controller.

Further, the incident angle of the incident ray is greater than fifty-six degrees and less than ninety degrees.

Further, the wavelength of the incident light is larger than or equal to 700nm and smaller than or equal to 900nm.

Further, the wavelength of the incident light is 850nm.

Further, the first angle is forty degrees or more and fifty degrees or less.

Further, the first angle is forty-five degrees.

Furthermore, the focus locking system further comprises a reflecting mirror, the reflecting mirror is arranged between the beam expanding mirror group and the third lens, and the reflecting mirror is used for turning the light path.

Further, the detector is a linear array CCD.

The utility model also discloses a microscope, use foretell lock burnt system.

Further, the focus locking system is arranged on the upper side of the objective table.

The embodiment of the utility model provides a microscope imaging lock burnt system and microscope compares with prior art, and its beneficial effect lies in: the utility model discloses a lock burnt system of incident light through objective incident not, the dependence and the influence to the micro-imaging system can be ignored when locking burnt; the coke locking system has fewer devices and simple mechanism, reduces the processing, assembly and adjustment difficulty and is a modular system which is easier to realize; the utility model discloses a lock burnt system can compatible multiple microscope, and need not change microscopical structure.

Drawings

Fig. 1 is a first structural schematic diagram of a microscopic imaging focusing system according to the present invention;

fig. 2 is a second structural schematic diagram of the microscopic imaging focusing system of the present invention;

fig. 3 is a schematic diagram of optical path propagation when the slide glass is displaced in the vertical direction in the microscope imaging focusing system of the present invention.

In the figure, 1, objective lens; 2. an object stage; 3. the lower surface of the glass slide; 4. an upper surface of the slide; 5. a light source; 6. a first lens; 7. a second lens; 8. a mirror; 9. a third lens; 10. a detector; 11. an incident light beam; 12. reflecting the light beam; 13. the light beam is fed back.

Detailed Description

The following detailed description of the embodiments of the present invention is made with reference to the accompanying drawings and examples. The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention.

As shown in fig. 1, the utility model discloses a micro-imaging focusing system, which comprises a light source 5, a first lens 6, a second lens 7, a third lens 9, a detector 10 and an objective lens displacement controller; the light source 5 is used for generating incident light, and the first lens 6, the second lens 7 and the third lens 9 are sequentially arranged on a propagation path of a reflected light beam 12, wherein the reflected light beam 12 is formed by reflecting the incident light beam 11 through the upper surface 4 of the glass slide; the first lens 6 and the second lens 7 form a beam expander set, the beam expander set is used for amplifying offset, the third lens 9 is used for converging a reflected light beam 12 to a detector 10 arranged at a first angle, the detector 10 is used for acquiring the position of a light spot, and the detector 10 is connected with an objective lens displacement controller.

Those skilled in the art will appreciate that the focus lock system can be used alone as a module of a microscope and can be used with a variety of different microscope models. When using the focus locking system of the present invention, a person skilled in the art can mount the focus locking system on the stage 2 of the microscope by himself, as shown in fig. 1 and 2, and the microscope is mounted on the upper side of the stage 2. Using a microscope, one skilled in the art installs the slide himself and makes corrections. There are various technical means in the art for adjusting the height or position of the objective lens, and these devices may be referred to as objective lens displacement controllers for controlling the movement of the objective lens 1.

In this embodiment, the probe light reflected by the lower surface 3 of the slide passes through the entire sample, which results in a large loss, and secondly, the sample is complicated in terms of various substances, and the probe light is reflected and scattered unpredictably by the substances, so that only the upper surface 4 of the slide is considered when implementing the technical solution of the present invention.

The specific implementation mode is as follows: firstly, correction is carried out, a light source 5 is turned on, incident light rays emitted by the light source 5 are reflected by the upper surface 4 of the glass slide to form reflected light rays, and the reflected light rays sequentially pass through a first lens 6, a second lens 7 and a third lens 9 and then are converged on a detector 10 to form initial light spots and record the initial positions of the initial light spots.

After the correction is completed, the slide may be displaced due to the influence of external factors. When the slide generates vertical displacement, incident light forms a first light spot on the detector 10, the detector 10 acquires a first position of the first light spot, compares the first position with an initial position, and sends a first control signal to the objective lens displacement controller according to the first position and the initial position, and the objective lens displacement controller controls the movement of the objective lens 1 according to the first control signal.

As shown in fig. 3, the slide is axially displaced in Δ z dimension, and the feedback beam 13 is focused on the detector 10 via the subsequent optical path, and the first position is separated from the initial position by Δ d. By controlling the movement of the objective lens 1, the deviation is calibrated, so that the light spot returns to the initial position again, and the purpose of locking the focal plane in the process of microscope imaging is further realized.

In the present embodiment, the incident angle of the incident ray is greater than fifty-six degrees and less than ninety degrees.

In this embodiment, the few microscopes that are also required to be set according to the mechanical parameters of the microscope that are equipped may exceed the above-mentioned range of incidence angles, and therefore in special cases, the incidence angle is greater than zero and less than ninety degrees.

In this embodiment, the beam expander set only needs to select two lenses, and a person skilled in the art can further add a proper amount of lenses according to actual needs, so as to further add one lens on this basis to improve the amplification effect of the offset, where the offset is the offset of the slide. The multiple focuses of the multiple lenses in the beam expander set have the best effect of amplifying offset when being on the same straight line. Therefore, those skilled in the art can arrange the plurality of lenses of the beam expander group in a state that the focal points are in the same straight line.

In this embodiment, the wavelength of the incident light is equal to or greater than 700nm and equal to or less than 900nm.

In this embodiment, the incident light has a wavelength of 850nm.

In this embodiment, the first angle is forty degrees or more and fifty degrees or less.

In this embodiment, the first angle is forty-five degrees.

In this embodiment, since the system uses the lateral movement amount of the detection feedback signal (the first light spot), the detection sensitivity of the system also depends on the pixel size of the detector 10 (a variation Δ z needs to occupy at least one pixel size to be resolved), and the detector 10 is tilted by forty-five degrees to improve the detection sensitivity of the system by 1/√ 2 times, so that 45 ° is selected instead of other angles, because the shape of the light spot formed on the detector 10 by the feedback signal of the upward/downward deviation of the sample when placed at 45 ° is symmetrically changed, and such light spot change can ensure the dynamic range of the focus-locked system.

Those skilled in the art know that there are no angles other than 0 ° at which a symmetrical spot can be achieved. For the detector 10, the smaller the size of the acquired spot, the higher the reading accuracy (the spot is larger than one pixel size), and when the sample deviates from the locked position, the spot will become larger no matter it deviates upward or downward (for example, a symmetrical spot size will ensure that the dynamic range is ± 5um, instead of-2 to +8 um). In practical application, deviation is random, so that the dynamic range of the system can be ensured by selecting the inclination angle of forty-five degrees.

In this embodiment, the focus locking system further includes a reflecting mirror 8, the reflecting mirror 8 is disposed between the beam expanding mirror group and the third lens 9, and the reflecting mirror 8 is used for deflecting the light path. The reflector 8 is mainly used to fold the light path to avoid the system being too long.

In this embodiment, the detector 10 is a linear array CCD. Those skilled in the art can select other types or kinds of photodetectors 10, and achieve the corresponding technical effects according to the technical solutions disclosed in the present invention.

The utility model also discloses a microscope, use foretell lock burnt system.

In this embodiment, the focus lock system is mounted on the upper side of the stage 2.

In this embodiment, the microscope may be an inverted fluorescence microscope. The utility model discloses a lock burnt system can cooperate the microscope of multiple model to not confine to certain microscope.

To sum up, the embodiment of the utility model provides a microscope imaging lock burnt system and microscope compares with prior art, and its beneficial effect lies in: the utility model discloses a lock burnt system of incident light through objective incident not, the dependence and the influence to the micro-imaging system can be ignored when locking burnt; the coke locking system has fewer devices and simple mechanism, reduces the processing, assembly and adjustment difficulty and is a modular system which is easier to realize; the utility model discloses a lock burnt system can compatible multiple microscope, and need not change microscopical structure.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and replacements can be made without departing from the technical principle of the present invention, and these modifications and replacements should also be considered as the protection scope of the present invention.

Claims (10)

1. A microscopic imaging focus-locking system is characterized by comprising a light source, a first lens, a second lens, a third lens, a detector and an objective lens displacement controller; the light source is used for generating incident light, the first lens, the second lens and the third lens are sequentially arranged on a propagation path of a reflected light beam, and the reflected light beam is formed by reflecting the incident light beam on the upper surface of the glass slide; the beam expander set is composed of the first lens and the second lens and used for amplifying offset, the third lens is used for converging a reflected light beam to a detector arranged at a first angle, the detector is used for acquiring the position of a light spot, and the detector is connected with the objective lens displacement controller.

2. A microscopic imaging lock focus system according to claim 1, wherein said incident light has an angle of incidence greater than fifty-six degrees and less than ninety degrees.

3. A microscopic imaging focusing system according to claim 2, wherein said incident light has a wavelength of 700nm or more and 900nm or less.

4. A microscopic imaging lock focus system according to claim 1, wherein said incident light has a wavelength of 850nm.

5. The microscopic imaging focusing system according to claim 1, wherein said first angle is forty degrees or more and fifty degrees or less.

6. The microscopic imaging focusing system according to claim 5, wherein said first angle is forty-five degrees.

7. The microscopic imaging focusing system according to claim 1, further comprising a reflecting mirror disposed between the beam expander set and the third lens, wherein the reflecting mirror is used for deflecting the light path.

8. The microscopic imaging focusing system according to claim 1, wherein the detector is a line CCD.

9. A microscope, characterized in that a focus locking system according to any one of claims 1-8 is applied.

10. A microscope according to claim 9, wherein the focus lock system is mounted on the upper side of the stage.

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