CN217981259U - Program-controlled fluorescence detection sensor - Google Patents

Abstract

The application relates to a program-controlled fluorescence detection sensor, which belongs to the technical field of fluorescence detection and sensing, and in practical application, the program-controlled fluorescence detection sensor comprises a detector main body, shading cotton and a side cover, wherein a fluorescence receiving circuit board, a main control board and a light source are arranged outside the detector main body, and the main control board is respectively electrically connected with the fluorescence receiving circuit board and the light source; a light source collimating lens, a light homogenizing sheet, an excitation filter and a refraction lens forming an angle of 45 degrees with the horizontal direction are sequentially arranged in the detector main body along the horizontal direction; a focusing lens group, an emission filter and a fluorescence collection lens are sequentially arranged in the detector main body along the vertical direction; and the fluorescence receiving circuit board is provided with a photoelectric probe. The invention is beneficial to solving the technical problems of interference caused by antenna effect of analog signals, difficulty in adapting to complex working condition environment, difficulty in meeting the requirement of rapid field test and higher cost.

Description

Program-controlled fluorescence detection sensor

Technical Field

The application belongs to the technical field of fluorescence detection and sensing, and particularly relates to a program-controlled fluorescence detection sensor.

Background

In recent years, with the rapid development of biotechnology, microfluidics technology, artificial intelligence and other related technologies, the demand for fluorescence-induced detection technology in the fields of microfluidics, biomedical detection, biomolecule detection, drug screening, environmental detection, biochemical warfare agent detection, bioaerosol detection and the like is rising.

The induced fluorescence method is a method for exciting a fluorescent substance in a sample by incident light to emit fluorescence and performing photoelectric conversion by a photoelectric detector, and has the advantages of high detection sensitivity, simplicity in operation and the like.

The existing fluorescent detection scheme and production are too complex, the size is large, and the assembly difficulty is high. At present, most of fluorescent detectors on the market are designed with a light path, a detection circuit and a control circuit separately, the analog quantity of a fluorescent signal needs to pass through a long lead, interference caused by the antenna effect of the analog signal is difficult to remove, the fluorescent detectors are difficult to adapt to complex working condition environments, the requirements of rapid field tests are difficult to deal with, and the cost is high.

Disclosure of Invention

Therefore, the application provides a program-controlled fluorescence detection sensor to solve the technical problems that interference caused by an antenna effect of an analog signal is difficult to adapt to a complex working condition environment, the requirement of rapid field test is difficult to meet, and the cost is high.

In order to achieve the purpose, the following technical scheme is adopted in the application:

the application provides a programme-controlled fluorescence detection sensor, includes: a detector main body, a shading cotton and a side cover,

a fluorescence receiving circuit board, a main control board and a light source are arranged outside the detector main body, and the main control board is electrically connected with the fluorescence receiving circuit board and the light source respectively;

a light source collimating lens, a light homogenizing sheet, an excitation filter and a refraction lens forming an angle of 45 degrees with the horizontal direction are sequentially arranged in the detector main body along the horizontal direction;

a focusing lens group, an emission filter and a fluorescence collection lens are sequentially arranged in the detector body along the vertical direction;

and the fluorescence receiving circuit board is provided with a photoelectric probe.

Further, the dioptric lens is a spectroscope or a dichroic mirror.

Further, the photoelectric probe is a PD photodiode, a PIN photodiode, an APD avalanche photodiode, a SiPM phototube or a CMOS detector.

Further, the light source is an LED light source, a laser or a halogen tungsten bulb.

Further, the detector main body and the side cover are both formed by injection molding or CNC machining.

Furthermore, the fluorescence receiving circuit board comprises a preamplification circuit, a low-pass filter circuit, an ADC circuit module, a microcontroller and a signal transmission circuit module which are electrically connected in sequence.

This application adopts above technical scheme, possesses following beneficial effect at least: the device has smaller volume, compact structure and simple production method, and is convenient for batch production, graded quality inspection and maintenance.

In this application practical application, the light that sends through the light source becomes collimated monochromatic light source through light source collimating lens, even slide, excitation filter, obtains the excitation light beam of vertical direction after refraction lens deflection. The excitation light beam in the vertical direction forms a convergent excitation light point through the focusing lens group, so that the light energy is conveniently intensively excited and is irradiated on the sample to be measured. Therefore, the technical problems that the interference caused by the antenna effect of the analog signal is difficult to adapt to a complex working condition environment, the requirement of rapid field test is difficult to deal with, and the cost is high are solved.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the application.

Drawings

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic diagram of a system for programming a fluorescence detection sensor according to an exemplary embodiment;

FIG. 2 is an exploded view of a programmable fluorescence detection sensor according to an exemplary embodiment;

FIG. 3 is a functional block diagram of circuitry of a programmable fluorescence detection sensor according to an exemplary embodiment.

In the figure, 1-a detector body, 101-a light source, 102-a light source collimating lens, 103-a light homogenizing sheet, 104-an excitation filter, 105-a refraction lens, 106-a focusing lens group, 107-an emission filter, 108-a fluorescence collecting lens, 2-a fluorescence receiving circuit board, 201-a photoelectric probe, 3-a main control board, 4-shading cotton and 5-a side cover.

Detailed Description

In order to make the objects, technical solutions and advantages of the present application more apparent, the technical solutions of the present application will be described in detail below. It is to be understood that the embodiments described are only a few embodiments of the present application and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without making any creative effort, shall fall within the protection scope of the present application.

Referring to fig. 1 and 2, in a preferred embodiment of the present invention, a programmable fluorescence detection sensor comprises: the detector comprises a detector main body 1, a fluorescence receiving circuit board 2, a main control board 3, shading cotton 4 and a side cover 5; the side wall of the detector body 1 is provided with a light source 101; a light source collimating lens 102, a light homogenizing sheet 103 and an excitation filter 104 are sequentially arranged in the detector body 1 from right to left along the horizontal direction, a refraction lens 105 is arranged at an angle of 45 degrees with the horizontal direction, and a focusing lens group 106, an emission filter 107 and a fluorescence collecting lens 108 are arranged in the detector body 1 from bottom to top along the vertical direction; the upper part of the detector body 1 is provided with a photoelectric probe 201, and a fluorescence receiving circuit board 2 is arranged corresponding to the photoelectric probe 201; the fluorescence receiving circuit board 2 is electrically connected with the main control board 3 through a lead; the light source 101 is electrically connected with the main control panel 3 through a wire.

In practical applications, light emitted from the light source 101 passes through the light source collimating lens 102, the light homogenizing sheet 103, and the excitation filter 104 to become a collimated monochromatic light source, and is deflected by the refraction lens 105 to obtain an excitation beam in a vertical direction. The excitation light beam in the vertical direction forms a convergent excitation light point through the focusing lens group 106, so that the excitation light energy is conveniently concentrated and is irradiated on the sample to be measured. According to the reversibility of the optical path, the fluorescence excited by the sample to be detected reaches the photoelectric probe 201 through the focusing lens group 106, the emission filter 107 and the fluorescence collecting lens 108, and finally the signal conversion is completed on the fluorescence receiving circuit board 2.

In one embodiment, the optical lens can be any one of a dichroic mirror or a dichroic mirror, which can be more selective.

In one embodiment, the photo-probe may be any one of a PD photodiode, a PIN photodiode, an APD avalanche photodiode, a SiPM photo-tube, or a CMOS detector, which may be more selective.

In one embodiment, the light source is any one of an LED light source, a laser or a tungsten halogen bulb, which may be more selective.

In one embodiment, the detector body and the side cover are both injection molded or CNC machined, which can reduce production costs.

Referring to fig. 3, the fluorescence receiving circuit board 2 includes a pre-amplification circuit, a low-pass filter circuit, an ADC circuit module, a microcontroller and a signal transmission circuit module, which are electrically connected in sequence. The fluorescent light signal can be converted into an electrical signal.

In practical application, the photoelectric probe 201 obtains a fluorescence signal, the fluorescence signal is converted into a digital fluorescence signal through the pre-amplification circuit, the low-pass filter circuit and the ADC circuit module, and the digital fluorescence signal is sent to an MCU microcontroller (STM 32) for data processing. The processed data is transmitted to the signal interface through a signal transmission circuit module, such as an RS232 or RS485 circuit module. The control signal is transmitted to the MCU (STM 32) through the signal interface and the signal transmission circuit module, the current required by the output of the constant current module of the numerical control DAC is controlled, and then the program control output module is controlled to output a corresponding constant current to the light source 101, so that the light source 101 can be controlled to emit light or be switched off. This is done the utility model discloses a process of whole fluorescence signal collection, excitation light source control. In the process, the power interface can obtain a relatively pure power through the circuit filter circuit module to provide electric energy for each circuit.

Therefore, the utility model relates to a programme-controlled fluorescence detection sensor is through integrated exciting light, light beam collimation, even light, extinction, fluorescence collection, fluorescence detection, signal filtering in an organic whole, equipment compact structure and easily operation, this equipment possesses small, compact structure, and production method is simple, the batch production of being convenient for, hierarchical quality control and maintenance. The interference caused by the antenna effect of the analog signal is solved, the antenna device can adapt to complex working condition environments, the requirement of rapid field test can be met, and the main structure is formed by injection molding or CNC machining, so that the production cost is fully reduced.

It is understood that the same or similar parts in the above embodiments may be mutually referred to, and the same or similar parts in other embodiments may be referred to for the content which is not described in detail in some embodiments.

It should be noted that, in the description of the present application, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. In addition, in the description of the present application, the meaning of "plurality" means at least two unless otherwise specified.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present; when an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present, and further, as used herein, connected may include wirelessly connected; the term "and/or" is used to include any and all combinations of one or more of the associated listed items.

Any process or method descriptions in the flow charts or otherwise described herein may be understood as: represents modules, segments or portions of code which include one or more executable instructions for implementing specific logical functions or steps of a process, and the scope of the preferred embodiments of the present application includes other implementations in which functions may be executed out of order from that shown or discussed, including substantially concurrently or in reverse order, depending on the functionality involved, as would be understood by those reasonably skilled in the art of the embodiments of the present application.

It should be understood that portions of the present application may be implemented in hardware, software, firmware, or a combination thereof. In the above embodiments, the various steps or methods may be implemented in software or firmware stored in memory and executed by a suitable instruction execution system. For example, if implemented in hardware, as in another embodiment, any one or combination of the following techniques, which are known in the art, may be used: a discrete logic circuit having a logic gate circuit for implementing a logic function on a data signal, an application specific integrated circuit having an appropriate combinational logic gate circuit, a Programmable Gate Array (PGA), a Field Programmable Gate Array (FPGA), or the like.

It will be understood by those skilled in the art that all or part of the steps carried by the method for implementing the above embodiments may be implemented by hardware related to instructions of a program, which may be stored in a computer readable storage medium, and when the program is executed, the program includes one or a combination of the steps of the method embodiments.

In addition, functional units in the embodiments of the present application may be integrated into one processing module, or each unit may exist alone physically, or two or more units are integrated into one module. The integrated module can be realized in a hardware mode, and can also be realized in a software functional module mode. The integrated module, if implemented in the form of a software functional module and sold or used as a stand-alone product, may also be stored in a computer readable storage medium.

The storage medium mentioned above may be a read-only memory, a magnetic or optical disk, etc.

In the description of the present specification, reference to the description of "one embodiment," "some embodiments," "an example," "a specific example," or "some examples" or the like means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present application. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

Although embodiments of the present application have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present application, and that variations, modifications, substitutions and alterations may be made to the above embodiments by those of ordinary skill in the art within the scope of the present application.

Claims (6)

1. A programmable fluorescence detection sensor, comprising: detector main part, shading cotton and side cap, its characterized in that:

a fluorescence receiving circuit board, a main control board and a light source are arranged outside the detector main body, and the main control board is electrically connected with the fluorescence receiving circuit board and the light source respectively;

a light source collimating lens, a light homogenizing sheet, an excitation filter and a refraction lens forming an angle of 45 degrees with the horizontal direction are sequentially arranged in the detector body along the horizontal direction;

a focusing lens group, an emission filter and a fluorescence collection lens are sequentially arranged in the detector main body along the vertical direction;

and the fluorescence receiving circuit board is provided with a photoelectric probe.

2. The programmable fluorescence detection sensor of claim 1, wherein the refractive optic is a dichroic mirror or a dichroic mirror.

3. The programmable fluorescence detection sensor of claim 1, wherein said photodetector is a PD photodiode, a PIN photodiode, an APD avalanche photodiode, a SiPM photocell, or a CMOS detector.

4. The programmable fluorescence detection sensor of claim 1, wherein the light source is an LED light source, a laser, or a tungsten halogen bulb.

5. The programmable fluorescence detection sensor of claim 1, wherein said detector body and said side cover are injection molded or CNC machined.

6. The programmable fluorescence detection sensor of claim 1, wherein the fluorescence receiving circuit board comprises a pre-amplifying circuit, a low-pass filter circuit, an ADC circuit module, a microcontroller and a signal transmission circuit module which are electrically connected in sequence.

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