CN217980981U

CN217980981U - Sample concentration cavity of extracellular vesicle

Info

Publication number: CN217980981U
Application number: CN202221875801.9U
Authority: CN
Inventors: 安瑾; 樊萌; 刘轩
Original assignee: Beijing Yimicroorganism Technology Co ltd
Current assignee: Beijing Yimicroorganism Technology Co ltd
Priority date: 2022-07-20
Filing date: 2022-07-20
Publication date: 2022-12-06
Anticipated expiration: 2032-07-20

Abstract

The utility model relates to an extracellular vesicle technical field just discloses a concentrated cavity of sample of extracellular vesicle, including the cavity, the cavity includes chamber lid, air pump, gas-supply pipe, sieve, water absorption plate, spacing ring, goes up cavity, lower cavity and sealing washer. This concentrated cavity of sample of extracellular vesicle is through installing cavity down, go up the cavity, the spacing ring, the sealing washer, sieve and the board that absorbs water, when the board that absorbs water failure, cavity and last cavity separation down can be with cavity under rotatory, take out the spacing ring from cavity screw thread bulge down again, can take out the sieve and absorb water the board, during the replacement, place the sieve that the top surface fixed mounting had the board that absorbs water in the spacing ring department of cavity down, pass cavity screw thread bulge down with the spacing ring opening again and place in cavity top surface down, cavity and last cavity threaded connection down again, let the spacing ring fix the sieve and absorb water the board, let whole cavity can use repeatedly.

Description

Sample concentration cavity of extracellular vesicle

Technical Field

The utility model relates to an extracellular vesicle technical field specifically is a concentrated cavity of sample of extracellular vesicle.

Background

Vesicles are amphiphilic molecules, such as many natural synthetic surfactants and phospholipids that do not simply associate into micelles, that spontaneously form ordered assemblies of molecules with a closed bilayer structure when dispersed in water, also known as liposomes. General outer vesicle model need can only experiment after the concentration, and current outer vesicle sample concentration cavity can lose the effect of absorbing water because its board that absorbs water can not be replaced after absorbing water many times, and the board that absorbs water is fixed mounting in the cavity inner wall mostly, and inconvenient the replacement of taking out can only replace whole cavity for the cavity can not use repeatedly, has increased use cost.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

The utility model provides a not enough to prior art, the utility model provides a concentrated cavity of sample of extracellular vesicle, the utensil is equipped with down the cavity, go up the cavity, the spacing ring, the sealing washer, sieve and water absorption plate, make when water absorption plate absorbs water and became invalid, the cavity can be with cavity and last cavity separation down through rotatory lower cavity, take out the spacing ring from cavity screw thread bulge down again, can take out the sieve and replace with the water absorption plate, during the replacement, place the sieve that top surface fixed mounting had the water absorption plate in the spacing collar department of cavity down, pass cavity screw thread arch down with the spacing ring opening again and place in cavity top surface down, cavity and last cavity threaded connection down again, because setting up of sealing washer makes cavity and last cavity junction sealed down, and the spacing ring fixes the sieve and the water absorption plate, let advantages such as whole cavity can use repeatedly, the technical problem is solved.

(II) technical scheme

In order to achieve the above object, the utility model provides a following technical scheme: a sample concentration cavity of extracellular vesicles comprises a cavity body, wherein the cavity body comprises a cavity cover, an air pump, an air conveying pipe, a sieve plate, a water absorption plate, a limiting ring, an upper cavity body, a lower cavity body and a sealing ring;

the upper cavity top surface is provided with a cavity cover, the lower cavity is arranged on the bottom surface of the upper cavity, a sieve plate is arranged on the inner wall of the lower cavity, and a water absorption plate is arranged on the top surface of the sieve plate.

Preferably, it is equipped with the screw thread arch around opening and the opening to go up the cavity top surface, the cavity inner wall is equipped with the screw thread, cavity lid and last cavity screw thread arch threaded connection, the cavity bottom surface is equipped with opening and inner wall and is equipped with the screw thread down, the cavity top surface is equipped with opening and top surface and is equipped with the screw thread arch down, cavity and last cavity threaded connection down, the cavity bottom surface is equipped with opening and is equipped with sealed lid down.

Through the technical scheme, the top surface of the upper cavity is provided with the opening, the periphery of the opening is provided with the thread protrusion, the inner wall of the cavity cover is provided with the thread, the cavity cover is in threaded connection with the thread protrusion of the upper cavity, the bottom surface of the lower cavity is provided with the opening, the inner wall of the lower cavity is provided with the thread protrusion, the top surface of the lower cavity is provided with the opening, the top surface of the lower cavity is provided with the thread protrusion, the lower cavity is in threaded connection with the upper cavity, the bottom surface of the lower cavity is provided with the opening, the sealing cover is arranged on the opening, the upper cavity and the lower cavity can be connected through the mutual threads to form a complete cavity, the disassembly is convenient, and the cavity cover and the sealing cover with the opening on the bottom surface of the lower cavity can seal the space formed by the upper cavity and the lower cavity.

Preferably, the inner wall of the lower cavity body is provided with a limiting ring, the sieve plate is arranged on the top surface of the lower cavity body limiting ring, the top surface of the sieve plate is fixedly provided with a water absorption plate, the water absorption plate is made of super water absorption materials, and the surface of the water absorption plate is provided with a plurality of holes in a penetrating mode.

Through above-mentioned technical scheme, be equipped with the spacing collar through cavity of resorption internal wall, the sieve is placed in cavity of resorption spacing collar top surface, sieve top surface fixed mounting has the water absorption plate, the water absorption plate is made for super absorbent material and the water absorption plate surface runs through and is equipped with a plurality of holes for the extracellular vesicle sample accessible water absorption plate that gets into from last cavity top surface opening is concentrated by absorbed moisture, partial waste liquid can be through the sieve flow in cavity and concentrated extracellular vesicle sample can be filtered by the sieve and stay on the water absorption plate surface.

Preferably, the surface of the limit ring is provided with a circle of opening, the opening of the limit ring penetrates through the thread bulge of the lower cavity and is arranged on the top surface of the lower cavity, and the top surface of the limit ring is fixedly provided with a sealing ring.

Through the technical scheme, the round opening is formed in the surface of the limiting ring, the limiting ring opening penetrates through the lower cavity thread protrusion to be placed on the top surface of the lower cavity, the sealing ring is fixedly mounted on the top surface of the limiting ring, so that the water absorption plate and the sieve plate can be fixed by the limiting ring when the lower cavity is in threaded connection with the upper cavity, and the connection part of the lower cavity and the upper cavity is sealed through the arrangement of the sealing ring.

Preferably, the top surface of the cavity cover is provided with a hole, one end of the gas pipe is fixedly connected with the hole on the top surface of the cavity cover, and the other end of the gas pipe is fixedly connected with the air pump.

Through above-mentioned technical scheme, be equipped with the hole through chamber lid top surface, gas-supply pipe one end and chamber lid top surface hole fixed connection, the gas-supply pipe other end and air pump fixed connection for the air pump can be with gaseous in the space of cavity and lower cavity constitution is gone up by the gas-supply pipe input, makes extracellular vesicle sample dehydration speed accelerate, improves extracellular vesicle sample concentration efficiency.

Compared with the prior art, the utility model provides a concentrated cavity of sample of extracellular vesicle possesses following beneficial effect:

1. the utility model discloses an install cavity down, go up the cavity, the spacing ring, the sealing washer, sieve and water absorption plate, make when water absorption plate absorbs water and became invalid, cavity and last cavity separation down can be with cavity down through rotatory down, take out the spacing ring from cavity screw thread bellying down again, can take out the sieve and absorb water the board and replace, during the replacement, place the top surface fixed mounting sieve that absorbs water the board in the spacing collar department of cavity down, pass cavity screw thread arch down with the spacing ring opening again and place in cavity top surface down, cavity and last cavity threaded connection down again, because setting up of sealing washer makes cavity and last cavity junction sealed down, and the spacing ring is fixed the sieve and is absorbed water the board, let whole cavity can use repeatedly.

2. The utility model discloses an installation air pump and gas-supply pipe for in the air pump can be gaseous the space of cavity and lower cavity constitution by the gas-supply pipe input, make extracellular vesicle sample dehydration speed accelerate, improve extracellular vesicle sample concentration efficiency.

Drawings

FIG. 1 is a front view of the structure of the present invention;

FIG. 2 is a schematic sectional view of the lower cavity of the present invention;

FIG. 3 is a partially enlarged schematic view of A in FIG. 1;

fig. 4 is a partially enlarged schematic view of B in the structure diagram of fig. 2 of the present invention.

Wherein: 1. a cavity; 2. a chamber cover; 3. an air pump; 4. a gas delivery pipe; 5. a sieve plate; 6. a water absorption plate; 7. a limiting ring; 8. an upper cavity; 9. a lower cavity; 10. and (5) sealing rings.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts all belong to the protection scope of the present invention.

Referring to fig. 1-4, a sample concentration chamber for extracellular vesicles comprises a chamber 1, wherein the chamber 1 comprises a chamber cover 2, an air pump 3, an air delivery pipe 4, a sieve plate 5, a water absorption plate 6, a limiting ring 7, an upper chamber 8, a lower chamber 9 and a sealing ring 10;

the top surface of the upper cavity 8 is provided with a cavity cover 2, the bottom surface of the upper cavity 8 is provided with a lower cavity 9, the inner wall of the lower cavity 9 is provided with a sieve plate 5, and the top surface of the sieve plate 5 is provided with a water absorption plate 6.

Specifically, it is protruding to go up 8 top surfaces of cavity and be equipped with the screw thread around being equipped with opening and opening, and 2 inner walls of cavity lid are equipped with the screw thread, and cavity lid 2 and the protruding threaded connection of last 8 screw threads of cavity, and lower cavity 9 bottom surfaces are equipped with opening and inner wall and are equipped with the screw thread, and lower cavity 9 top surfaces are equipped with opening and top surface and are equipped with the screw thread arch, and lower cavity 9 and last 8 threaded connection of cavity, lower cavity 9 bottom surfaces are equipped with opening and are equipped with sealed lid. The advantage is, it is protruding to be equipped with the screw thread around opening and the opening through last 8 top surfaces of cavity, 2 inner walls of cavity lid are equipped with the screw thread, cavity lid 2 and the protruding threaded connection of last 8 screw threads of cavity, lower cavity 9 bottom surfaces are equipped with opening and inner wall and are equipped with the screw thread, lower cavity 9 top surfaces are equipped with opening and top surface and are equipped with the screw thread arch, lower cavity 9 and last 8 threaded connection of cavity, lower cavity 9 bottom surfaces are equipped with opening and are equipped with sealed lid, make and go up cavity 8 and the mutual threaded connection of lower cavity 9 accessible and constitute complete cavity 1, and convenient the dismantlement, cavity lid 2 can make the space seal of going up cavity 8 and cavity 9 constitution with the sealed lid of lower cavity 9 bottom surface opening.

Specifically, the inner wall of the lower cavity 9 is provided with a limiting ring, the sieve plate 5 is arranged on the top surface of the limiting ring of the lower cavity 9, the top surface of the sieve plate 5 is fixedly provided with a water absorption plate 6, and the water absorption plate 6 is made of super water absorption materials and is provided with a plurality of holes in a penetrating mode on the surface of the water absorption plate 6. The advantage is, be equipped with the spacing collar through lower cavity 9 inner wall, sieve 5 is placed in lower cavity 9 spacing collar top surface, 5 top surface fixed mounting of sieve have water absorption plate 6, water absorption plate 6 is made for super absorbent material and water absorption plate 6 surface runs through and is equipped with a plurality of holes, make from the outer vesicle sample accessible water absorption plate 6 of the outer vesicle sample accessible water absorption plate that goes into of 8 top surfaces opening of last cavity, partial waste liquid can be filtered by sieve 5 and stay water absorption plate 6 surfaces down cavity 9 and concentrated outer vesicle sample can be crossed.

Specifically, the surface of the limiting ring 7 is provided with a circle of opening, the opening of the limiting ring 7 penetrates through the thread protrusion of the lower cavity 9 and is placed on the top surface of the lower cavity 9, and the top surface of the limiting ring 7 is fixedly provided with a sealing ring 10. The advantage is, is equipped with the round opening through 7 surfaces on the spacing ring, and 9 screw thread bulges of cavity are placed in cavity 9 top surfaces down through 7 openings on the spacing ring, and 7 top surfaces fixed mounting of spacing ring have sealing washer 10 for when cavity 9 and the 8 threaded connection of last cavity down, spacing ring 7 can be fixed with absorbing water board 6 and sieve 5, and through the setting of sealing washer 10, let cavity 9 and last cavity 8 junction sealed down.

Specifically, the top surface of the cavity cover 2 is provided with a hole, one end of the gas pipe 4 is fixedly connected with the hole on the top surface of the cavity cover 2, and the other end of the gas pipe 4 is fixedly connected with the air pump 3. The advantage is, is equipped with the hole through 2 top surfaces of chamber lid, 4 one ends of gas-supply pipe and 2 top surfaces of chamber lid hole fixed connection, 4 other ends of gas-supply pipe and 3 fixed connection of air pump for air pump 3 can be with gas in the space of cavity 8 and lower cavity 9 constitution on the gas-supply pipe 4 input, make outer cell vesicle sample dehydration rate accelerate, improve outer cell vesicle sample concentration efficiency.

When the device is used, an extracellular vesicle sample is placed into the inner wall of an upper cavity 8 from an opening on the top surface of the upper cavity 8, then a cavity cover 2 is in threaded connection with a threaded bulge on the top surface of the upper cavity 8, external air is input into a space formed by the upper cavity 8 and a lower cavity 9 through an air conveying pipe 4 through an air pump 3, so that moisture of the extracellular vesicle sample is absorbed by a water absorption plate 6 at an accelerated speed, part of waste liquid enters the inner wall of the lower cavity 9 through a sieve plate 5 and the extracellular vesicle sample is left on the surface of the water absorption plate 6, after the extracellular vesicle sample is concentrated, the cavity cover 2 is opened to take out the extracellular vesicle sample, then a sealing cover on the bottom surface of the lower cavity 9 is opened to discharge the waste liquid, when the water absorption plate 6 needs to be replaced, the lower cavity 9 and the upper cavity 8 can be separated by rotating the lower cavity 9, then the limiting ring 7 is taken out from the threaded bulge of the lower cavity 9, the sieve plate 5 and the water absorption plate 6 can be taken out for replacement, when the water absorption plate 6 is replaced, the sieve plate 5 is placed at the limiting ring 7 and the upper cavity 9 and the sealing ring 9 is connected with the water absorption plate, and the sealing ring 9.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A sample concentration cavity of extracellular vesicles comprises a cavity (1) and is characterized in that: the cavity (1) comprises a cavity cover (2), an air pump (3), an air conveying pipe (4), a sieve plate (5), a water absorption plate (6), a limiting ring (7), an upper cavity (8), a lower cavity (9) and a sealing ring (10);

go up cavity (8) top surface and be equipped with chamber lid (2), it is equipped with down cavity (9) to go up cavity (8) bottom surface, cavity (9) inner wall is equipped with sieve (5) down, sieve (5) top surface is equipped with water absorption plate (6).

2. The extracellular vesicle sample concentration chamber according to claim 1, wherein: go up cavity (8) top surface and be equipped with the screw thread arch around opening and the opening, cavity lid (2) inner wall is equipped with the screw thread, cavity lid (2) and last cavity (8) screw thread arch threaded connection, cavity (9) bottom surface is equipped with opening and inner wall and is equipped with the screw thread down, cavity (9) top surface is equipped with opening and top surface and is equipped with the screw thread arch down, cavity (9) and last cavity (8) threaded connection down, cavity (9) bottom surface is equipped with opening and is equipped with sealed lid down.

3. The extracellular vesicle sample concentration chamber according to claim 1, wherein: cavity (9) inner wall is equipped with the spacing collar down, place in cavity (9) spacing collar top surface down in sieve (5), sieve (5) top surface fixed mounting has board (6) that absorb water, board (6) that absorb water are made for super absorbent material and absorb water board (6) surface and run through and be equipped with a plurality of holes.

4. The extracellular vesicle sample concentration chamber according to claim 1, wherein: the surface of the limiting ring (7) is provided with a circle of opening, the opening of the limiting ring (7) penetrates through the lower cavity (9) and is arranged on the top surface of the lower cavity (9) in a threaded protruding mode, and the top surface of the limiting ring (7) is fixedly provided with a sealing ring (10).

5. The extracellular vesicle sample concentration chamber according to claim 1, wherein: the top surface of the cavity cover (2) is provided with a hole, one end of the gas pipe (4) is fixedly connected with the hole on the top surface of the cavity cover (2), and the other end of the gas pipe (4) is fixedly connected with the air pump (3).