CN217970490U - Integrated nucleic acid extraction kit - Google Patents

Abstract

The utility model relates to an integral type nucleic acid extraction kit belongs to nucleic acid extraction device technical field. The technical scheme is provided mainly aiming at the problem that the existing nucleic acid extraction process is to separately operate four steps of cracking, combining, rinsing and eluting, which needs to use a plurality of kits: the sample injection device comprises a box body, wherein a plurality of partition plates are fixedly connected to the inside of the box body, the partition plates divide the inner space of the box body into a first chamber, a second chamber, a third chamber, a fourth chamber and a fifth chamber, the upper surfaces of the two ends of the box body are respectively and fixedly connected with a first bearing seat, a transverse screw shaft is rotatably connected between the first bearing seats, a transverse screw sleeve is spirally sleeved on the transverse screw shaft, and one side of the outer annular surface of the transverse screw sleeve is fixedly connected with a sample injection port through a short shaft. The kit is divided into five independent chambers in the kit body, and each chamber is pre-filled with different extraction reagents, so that four extraction operations can be simultaneously completed by one kit.

Description

Integrated nucleic acid extraction kit

Technical Field

The utility model relates to a nucleic acid extraction technical field especially relates to an integral type nucleic acid extraction kit.

Background

With the development of modern molecular biology technology, the leading research of life science is continuously active, the biotechnology revolutionized the world and accelerating the integration of the development into the economic society, and a brand-new solution is provided for the human beings to meet the major challenges of life health, climate change, resource and energy safety, grain safety and the like.

Nucleic acid extraction is the most important and fundamental technique in molecular biology. As the first step of nucleic acid detection, nucleic acid extraction and purification are needed for separating and extracting nucleic acid with high yield and high quality from complex and various biological samples rapidly and effectively, and are used as a means which is convenient to use, repeatable and scalable.

In the aspect of nucleic acid extraction reagents, the molecular detection industry mainly uses a traditional boiling method, an expensive imported magnetic bead method or a column extraction method in the past, the operation is complicated, and the accuracy and the sensitivity of reagent detection are poor; the international mainstream gene diagnosis products of the same kind mainly adopt the magnetic bead method technology (the fourth generation) to extract nucleic acid.

The extraction of nucleic acid extraction and purification process generally comprises four steps of cracking, combining, rinsing and eluting. Lysis refers to cell lysis to remove protein, polysaccharide, lipid and other biological macromolecules combined with nucleic acid; binding means precipitating the nucleic acid or adsorbing the nucleic acid, and binding the nucleic acid to a material capable of adsorbing the nucleic acid; rinsing refers to purifying nucleic acid, removing impurities such as salts and organic agents; elution refers to elution or dissolution of nucleic acids using an eluent or a dissolution solution.

The existing operation mode is to separately operate four steps, which requires a plurality of reagent boxes.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing an integral type nucleic acid extraction kit to the current nucleic acid extraction process mentioned in the background art is with four steps of schizolysis, combination, rinsing, elution separate operation respectively, this problem that just needs to use a plurality of kits.

The technical scheme of the utility model: the integrated nucleic acid extraction kit comprises a kit body, wherein a plurality of partition plates are fixedly connected to the inside of the kit body, the partition plates divide the inner space of the kit body into a first chamber, a second chamber, a third chamber, a fourth chamber and a fifth chamber, the upper surfaces of the two ends of the kit body are respectively and fixedly connected with a first bearing seat, a transverse screw shaft is rotatably connected between the two first bearing seats, the transverse screw shaft is spirally sleeved with a transverse screw sleeve, one side of the outer annular surface of the transverse screw sleeve is fixedly connected with a sample adding port through a short shaft, the bottom of the sample adding port is fixedly connected with a liquid transferring suction head through a hose, the upper surfaces of the two ends of the kit body are respectively and fixedly connected with a second bearing seat, a guide polished rod is connected between the two second bearing seats, the guide polished rod is movably sleeved, and the other side of the outer annular surface of the transverse screw sleeve is fixedly connected with the short shaft.

Preferably, the first connecting axle of bottom outer wall fixedly connected with of application of sample mouth, the one end fixedly connected with axle sleeve of application of sample mouth is kept away from to first connecting axle, the block is connected in the rotation of axle sleeve and the top of vertical spiral shell axle, vertical swivel nut has been cup jointed to the bottom spiral of vertical spiral shell axle, vertical swivel nut passes through minor axis and pipetting tip's outer wall fixed connection.

Preferably, the lower surface of the first connecting shaft is fixedly connected with a guide rod, and the bottom end of the guide rod movably penetrates through the second connecting shaft.

Preferably, the sample addition port, the hose and the pipette tip are communicated with each other.

Preferably, the sample adding port is sleeved with one end of a vent pipe, and the other end of the vent pipe is connected with the air flushing bag.

Preferably, an EP tube is disposed within the fifth chamber.

Compared with the prior art, the beneficial effects of the utility model are that:

(1): according to the kit, the five independent chambers are divided in the kit body, and each chamber is pre-filled with different extraction reagents, so that four extraction operations can be simultaneously completed by one kit;

(2): this application can shift the material in the different cavities through the design of horizontal spiral shell axle, vertical spiral shell axle isotructure.

Drawings

FIG. 1 is a schematic structural diagram of an integrated nucleic acid extraction kit;

fig. 2 is a sectional view of fig. 1.

Reference numerals are as follows: 1. a case body; 2. a partition plate; 3. a first bearing housing; 4. a transverse screw shaft; 5. a transverse threaded sleeve; 6. a sample addition port; 7. a hose; 8. pipetting tips; 9. a second bearing housing; 10. a guide polish rod; 11. a first connecting shaft; 12. a shaft sleeve; 13. a vertical screw shaft; 14. a vertical thread sleeve; 15. a second connecting shaft; 16. a guide bar; 17. a first chamber; 18. a second chamber; 19. a third chamber; 20. a fourth chamber; 21. a fifth chamber; 22. an EP tube; 23. a breather tube.

Detailed Description

The technical solution of the present invention will be further explained with reference to the accompanying drawings and specific embodiments.

Examples

The utility model provides a pair of integral type nucleic acid extraction kit.

As shown in fig. 1-2, the disposable endoscope sheath comprises a box body 1, four partition plates 2 are fixedly connected inside the box body 1, the partition plates 2 divide the inner space of the box body 1 into a first chamber 17, a second chamber 18, a third chamber 19, a fourth chamber 20 and a fifth chamber 21, and an EP tube 22 is arranged in the fifth chamber 21.

The equal fixedly connected with first bearing frame 3 of both ends upper surface of box body 1 rotates between two first bearing frames 3 and is connected with horizontal spiral shell axle 4, and horizontal swivel nut 5 has been cup jointed to horizontal spiral shell axle 4 spiral, and minor axis fixedly connected with sample addition port 6 is passed through to outer anchor ring one side of horizontal swivel nut 5, and the bottom of sample addition port 6 and the top fixed connection and the intercommunication of hose 7, the bottom of hose 7 and move liquid suction head 8 fixed connection and intercommunication.

The upper surfaces of the two ends of the box body 1 are fixedly connected with a second bearing seat 9, a guide polished rod 10 is connected between the two second bearing seats 9, the guide polished rod 10 is movably sleeved with a guide rod 24, and the other side of the outer annular surface of the transverse threaded sleeve 5 is fixedly connected with the guide rod 24 through a short shaft.

The bottom outer wall an organic whole of application of sample mouth 6 has linked firmly first connecting axle 11, and the one end an organic whole that first connecting axle 11 kept away from application of sample mouth 6 has linked firmly axle sleeve 12, and the annular keyway has been seted up to axle sleeve 12's inner circle, and the top outer wall an organic whole of vertical spiral shell axle 13 is equipped with the annular key piece that can rotate the block with annular keyway and be connected. The bottom end of the vertical screw shaft 13 is spirally sleeved with a vertical screw sleeve 14, and the vertical screw sleeve 14 is fixedly connected with the outer wall of the liquid-transferring suction head 8 through a short shaft.

The lower surface of the first connecting shaft 11 is fixedly connected with the top end of the guide rod 16, and the bottom end of the guide rod 16 movably penetrates through the second connecting shaft 15.

The bottom end of the vent pipe 23 extends into the sample adding port 6, the other end of the vent pipe 23 can be connected with an air flushing bag or other air blowing devices, and the air pressure in the liquid-transfering suction head 8 can be changed by pressing and loosening the air flushing bag, so that materials in the chamber can be sucked out and transferred.

The working principle of the embodiment is as follows:

the first step is as follows: adding a sample into the first chamber 17 through the sample adding port 6, pre-filling a lysate of the nucleic acid extraction reagent in the first chamber 17, extending one end of the vent pipe 23 into the first chamber from the sample adding port 6, and then introducing gas to blow the lysate so as to fully and uniformly mix the sample and the lysate;

the second step is that: the horizontal screw shaft 4 is rotated, the horizontal screw shaft 4 drives the horizontal screw sleeve 5 to move horizontally through the spiral sleeve between the horizontal screw sleeve 5 and the horizontal screw sleeve 5, the horizontal screw sleeve 5 drives the sample adding port 6 to move horizontally to the second chamber 18 through the short shaft, then the vertical screw shaft 13 is rotated, the vertical screw shaft 13 drives the liquid-transferring suction head 8 to move downwards through the spiral sleeve between the vertical screw sleeve 14 and the horizontal screw sleeve, negative pressure is generated at the end part of the liquid-transferring suction head 8 by pressing an air flushing bag at the end part of the vent pipe 23, so that the binding liquid and the magnetic beads pre-installed in the second chamber 18 are transferred into the liquid-transferring suction head 8, and the lower part of the liquid-transferring suction head 8 adsorbs the magnetic beads by placing a magnet;

the third step: the waste liquid in the first chamber 17 is moved into the second chamber 18 again by rotating the transverse screw shaft 4 and the vertical screw shaft 13 and pressing the air flushing bag;

the fourth step: moving the rinsing liquid pre-filled in the third chamber 19 to the liquid-transfering sucker 8, and blowing the liquid by pressing the air-washing bag to fully and uniformly mix the rinsing liquid and the magnetic beads to complete rinsing;

the fifth step: the waste liquid in the first chamber 17 is moved to the third chamber 19, and the liquid is blown by pressing the air flushing bag to fully and uniformly mix the eluent and the magnetic beads to complete elution;

and a sixth step: the eluent in the first chamber 17 is moved into the EP tube 22 to complete the nucleic acid extraction.

The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited thereto, and various changes can be made without departing from the gist of the present invention within the scope of knowledge possessed by those skilled in the art.

Claims (6)

1. The utility model provides an integral type nucleic acid extraction kit, includes box body (1), its characterized in that: the improved structure of the liquid transfer case is characterized in that a plurality of partition plates (2) are fixedly connected to the inside of the case body (1), the partition plates (2) divide the inner space of the case body (1) into a first chamber (17), a second chamber (18), a third chamber (19), a fourth chamber (20) and a fifth chamber (21), the upper surfaces of the two ends of the case body (1) are respectively and fixedly connected with a first bearing seat (3), a transverse screw shaft (4) is rotatably connected between the two first bearing seats (3), the transverse screw shaft (4) is spirally sleeved with a transverse screw sleeve (5), one side of the outer annular surface of the transverse screw sleeve (5) is fixedly connected with a sample adding port (6) through a short shaft, the bottom of the sample adding port (6) is fixedly connected with a liquid transfer suction head (8) through a hose (7), the upper surfaces of the two ends of the case body (1) are respectively and fixedly connected with a second bearing seat (9), a guide polish rod (10) is connected between the two second bearing seats (9), the guide polish rod (10) is provided with a guide rod (24), and the outer annular surface of the transverse screw sleeve (5) is fixedly connected with the other side of the guide rod.

2. The integrated nucleic acid extraction kit according to claim 1, wherein a first connecting shaft (11) is fixedly connected to the outer wall of the bottom end of the sample port (6), a shaft sleeve (12) is fixedly connected to one end, away from the sample port (6), of the first connecting shaft (11), the shaft sleeve (12) is rotatably and snap-connected to the top end of a vertical screw shaft (13), a vertical screw sleeve (14) is spirally sleeved on the bottom end of the vertical screw shaft (13), and the vertical screw sleeve (14) is fixedly connected to the outer wall of the liquid transfer pipette tip (8) through a short shaft.

3. The integrated nucleic acid extraction kit according to claim 2, wherein a guide rod (16) is fixedly connected to the lower surface of the first connecting shaft (11), and the bottom end of the guide rod (16) movably penetrates through the second connecting shaft (15).

4. The integrated nucleic acid extraction kit according to claim 1, wherein the sample port (6), the flexible tube (7) and the pipette tip (8) are communicated with each other.

5. The integrated nucleic acid extraction kit according to claim 1, wherein the sample addition port (6) is sleeved with one end of a vent tube (23), and the other end of the vent tube (23) is connected with an air flushing bag.

6. The integrated nucleic acid extraction kit according to claim 1, wherein an EP tube (22) is disposed in the fifth chamber (21).

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