CN217948144U - Imaging system instrument is cultivateed to living cell hypoxemia - Google Patents
Imaging system instrument is cultivateed to living cell hypoxemia Download PDFInfo
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- CN217948144U CN217948144U CN202220717085.5U CN202220717085U CN217948144U CN 217948144 U CN217948144 U CN 217948144U CN 202220717085 U CN202220717085 U CN 202220717085U CN 217948144 U CN217948144 U CN 217948144U
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- China
- Prior art keywords
- bracing piece
- living cell
- observation platform
- recess
- lower extreme
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- 238000003384 imaging method Methods 0.000 title claims abstract description 28
- 206010021143 Hypoxia Diseases 0.000 title claims abstract description 17
- 208000018875 hypoxemia Diseases 0.000 title abstract description 7
- 238000001514 detection method Methods 0.000 claims abstract description 22
- 239000011521 glass Substances 0.000 claims abstract description 11
- 238000012544 monitoring process Methods 0.000 claims abstract description 7
- 230000007954 hypoxia Effects 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 49
- 239000001301 oxygen Substances 0.000 claims description 7
- 229910052760 oxygen Inorganic materials 0.000 claims description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 6
- 239000000523 sample Substances 0.000 claims description 6
- 230000001146 hypoxic effect Effects 0.000 claims 4
- 238000007689 inspection Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 8
- 230000012292 cell migration Effects 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 31
- 239000007788 liquid Substances 0.000 description 9
- 238000004113 cell culture Methods 0.000 description 8
- 239000003814 drug Substances 0.000 description 6
- 230000010412 perfusion Effects 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000012634 optical imaging Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
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Abstract
The utility model discloses an imaging system instrument is cultivateed to living cell hypoxemia relates to living cell imager field, does not possess record all ring edge borders for solving current living cell imager, and the data that consequently leads to the record can have the deviation, is difficult for discovering external factor's problem. The observation platform rear end is provided with the bracing piece, and the bracing piece upper end is provided with the display screen, and first recess has been seted up to the observation platform upper end, is provided with transparent glass in the first recess, has seted up the second recess in the observation platform, is provided with fluorescence signal imaging device in the second recess, is provided with treater and controller in the observation platform, and the bracing piece lower extreme is provided with the monitoring head, and the bracing piece lower extreme is provided with temperature detection, and the bracing piece inner wall is provided with humidity detection, and bracing piece lower extreme swing joint has the movable cover. The living cell hypoxia culture imaging system instrument monitors the surrounding environment of living cells in the hypoxia culture process in real time through temperature detection and humidity detection, and the cell migration process is observed in real time through the fluorescent signal imaging device.
Description
Technical Field
The utility model relates to a living cell imager technical field specifically is a living cell hypoxia culture imaging system instrument.
Background
The living cell imager can scan and detect cells at multiple channels and multiple targets under the condition of not damaging the whole structure of the cells, and in addition, the living cell culture system can realize long-time living cell drift-free observation, asynchronous exposure, multiple automatic observation and the like, and multiple multilayer observation of the cells is realized.
The live cell imager needs to record and generate images at regular time in the cell culture process, and because slight change can be generated along with the change of the ambient temperature and humidity during cell culture, the existing live cell imager does not record the ambient environment, so that the recorded data can have deviation, and external factors are not easy to discover.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide an imaging system instrument is cultivateed to living cell hypoxemia to propose the living cell imager and do not possess record all ring edge borders in solving above-mentioned background art, consequently there is the deviation in the data that lead to the record, the difficult problem of discovering external factor.
In order to achieve the above purpose, the utility model provides a following technical scheme: the utility model provides a living cell hypoxia culture imaging system instrument, includes the observation stand, the observation stand rear end is provided with the bracing piece, upper end of the support bar is provided with the display screen, first recess has been seted up to the observation stand upper end, be provided with clear glass in the first recess, set up the second recess in the observation stand, be provided with fluorescence signal imaging device in the second recess, be provided with first treater and first controller in the observation stand, the bracing piece lower extreme is provided with the monitoring head, the bracing piece lower extreme is provided with temperature detection, the bracing piece inner wall is provided with humidity detection, bracing piece lower extreme swing joint has the movable cover.
Through adopting above-mentioned technical scheme, but establish the display screen real-time supervision and observe data in the observation platform, and transparent glass can be convenient for scan the active state of living cell from the below, and the temperature detection that sets up below the bracing piece can acquire the change of living cell at the culture process ambient temperature.
Further, the bracing piece lower extreme is equipped with accurate oxygen probe, the spout has all been seted up to second recess inner wall both sides, two all be equipped with the movable rod between the spout, the observation platform front end is provided with the switch, bracing piece inner wall fixedly connected with saddle, be provided with the second cylinder in the saddle, the second cylinder is surveyed swing joint with humidity.
By adopting the technical scheme, the movable rod can drive the fluorescence signal imaging device to move back and forth, so that the living cell states in different time periods can be conveniently scanned and recorded by moving back and forth.
Furthermore, a first processor and a first controller are arranged in the observation platform, a first air cylinder is arranged in the support rod, and the lower end of the first air cylinder is movably connected with the temperature detection device.
By adopting the technical scheme, the first processor can timely record the acquired data, the first controller can respectively control the opening of the equipment, and the temperature detection can monitor and record the temperature change in the living cell culture process in real time.
Furthermore, a second controller and a second processor are arranged in the movable rod.
By adopting the technical scheme, the system comprises the second controller and the second processor.
Furthermore, third symmetrical grooves are formed in the observation platform, the two third grooves are communicated with the sliding groove, screw rods are arranged in the two third grooves, sliding blocks are connected in the two screw rods in a threaded mode, and driving motors are arranged at the front ends of the two screw rods.
By adopting the technical scheme, the slide block can drive the fluorescent signal imaging device to move under the action of the screw rod and the driving motor.
Further, the inner wall of the supporting rod is movably connected with a water tank, a movable plate is movably connected in the water tank, a third air cylinder is arranged in the supporting rod, a water tank is arranged in the water tank, a water inlet pipe is arranged in the supporting rod, a water inlet is formed in one side of the water tank, a first water pump is arranged in the water tank, a fourth air cylinder is arranged in the supporting rod, the lower end of the second water pump is movably connected with the movable plate, a second water pump is arranged in the movable plate, and a sewer pipe is installed at the lower end of the movable plate.
Through adopting above-mentioned technical scheme, constitute an automatic perfusion structure by water tank, third cylinder, fourth cylinder, fly leaf and downcomer, intelligent operations such as can be convenient for equipment automatic reagent feeding trades liquid, and the velocity of flow of liquid is adjustable.
Compared with the prior art, the beneficial effects of the utility model are that:
1. this imaging system instrument is cultivateed to living cell hypoxemia through addding temperature detection and humidity detection can real-time supervision living cell cultivates the environment around the in-process at the hypoxemia for the supplementary data of taking notes cell change, make data more have the authenticity, but accurate oxygen probe accurate control O2 concentration, thereby solved the situation that the external cell of cultivateing can't the internal gaseous environment of true simulation.
2. According to the living cell hypoxia culture imaging system instrument, the fluorescent signal imaging device is arranged below the transparent glass, so that the living cell culture process can be recorded clearly and more comprehensively, the optical imaging technology can observe the real-time state of cells in the box body and take pictures for recording, the cell migration process can be observed in real time, and the data accuracy is improved;
3. this imaging system instrument is cultivateed to living cell hypoxemia comprises an automatic perfusion structure by water tank, third cylinder, fourth cylinder, fly leaf and downcomer, can be convenient for equipment automatic reagent feeding trade intelligent operation such as liquid, and the velocity of flow of liquid is adjustable.
Drawings
Fig. 1 is a schematic perspective view of the present invention;
fig. 2 is a schematic sectional structure of the present invention;
fig. 3 is a schematic top view of the present invention;
fig. 4 is an enlarged schematic structural diagram of the area a of fig. 2 according to the present invention.
In the figure: 1. an observation platform; 2. a support bar; 3. a display screen; 4. a first groove; 5. transparent glass; 6. a switch; 7. a monitoring head; 8. detecting the temperature; 9. a saddle; 10. detecting humidity; 11. a first processor; 12. a first controller; 13. a second groove; 14. a chute; 15. a movable rod; 16. A fluorescence signal imaging device; 17. a first cylinder; 18. a second cylinder; 19. a second controller; 20. A second processor; 21. a third groove; 22. a screw rod; 23. a slider; 24. a drive motor; 25. a movable cover; 26. a precision oxygen probe; 27. a water tank; 28. a movable plate; 29. a third cylinder; 30. A water tank; 31. a water inlet pipe; 32. a water inlet; 33. a first water pump; 34. a fourth cylinder; 35. a second water pump; 36. a sewer pipe.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and the described embodiments are only some embodiments, not all embodiments, of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
As shown in fig. 1: the rear end of the observation platform 1 is provided with a supporting rod 2, the upper end of the supporting rod 2 is provided with a display screen 3, the upper end of the observation platform 1 is provided with a first groove 4, transparent glass 5 is arranged in the first groove 4, the front end of the observation platform 1 is provided with a switch 6, the lower end of the supporting rod 2 is movably connected with a movable cover 25, the movable state of living cells can be conveniently scanned from the lower part through the transparent glass 5, and the movable cover 25 can cover a cultured vessel to form a sealed space.
As shown in fig. 2: a second groove 13 is formed in an observation platform 1, a fluorescence signal imaging device 16 is arranged in the second groove 13, a first processor 11 and a first controller 12 are arranged in the observation platform 1, a monitoring head 7 is arranged at the lower end of a support rod 2, a temperature detection 8 is arranged at the lower end of the support rod 2, a humidity detection 10 is arranged on the inner wall of the support rod 2, sliding grooves 14 are formed in two sides of the inner wall of the second groove 13, a movable rod 15 is arranged between the two sliding grooves 14, a support platform 9 is fixedly connected to the inner wall of the support rod 2, a second air cylinder 18 is arranged in the support platform 9, the second air cylinder 18 is movably connected with the humidity detection 10, the first processor 11 and the first controller 12 are arranged in the observation platform 1, a first air cylinder 17 is arranged in the support rod 2, the lower end of the first air cylinder 17 is movably connected with the temperature detection 8, a second controller 19 and a second processor 20 are arranged in the movable rod 15, a precise oxygen probe 26 is arranged at the lower end of the support rod 2, and the temperature detection 8 and the humidity detection 10 can monitor the environment around the living cells in the low-oxygen culture process in real time and can be used for assisting in recording the change of the cells, so that the authenticity of the data is improved.
As shown in fig. 3: symmetrical third grooves 21 are formed in the observation platform 1, the two third grooves 21 are communicated with the sliding groove 14, lead screws 22 are arranged in the two third grooves 21, sliding blocks 23 are connected in the two lead screws 22 in a threaded mode, driving motors 24 are arranged at the front ends of the two lead screws 22, the sliding blocks 23 can drive the fluorescent signal imaging devices 16 to move under the action of the lead screws 22 and the driving motors 24, the fluorescent signal imaging devices 16 are arranged below the transparent glass 5, the living cell culture process can be recorded clearly and more comprehensively, the real-time state of cells in the box can be observed through an optical imaging technology, the cell migration process can be observed in real time, and the accuracy of data is improved.
As shown in fig. 4: the inner wall of the support rod 2 is movably connected with a water tank 27, the water tank 27 is movably connected with a movable plate 28, the support rod 2 is internally provided with a third cylinder 29, the water tank 27 is internally provided with a water tank 30, the support rod 2 is internally provided with a water inlet pipe 31, one side of the water tank 27 is provided with a water inlet 32, the water tank 30 is internally provided with a first water pump 33, the support rod 2 is internally provided with a fourth cylinder 34, the lower end of the second water pump 35 is movably connected with the movable plate 28, the movable plate 28 is internally provided with a second water pump 35, the lower end of the movable plate 28 is provided with a sewer pipe 36, the water tank 27, the third cylinder 29, the fourth cylinder 34, the movable plate 28 and the sewer pipe 36 form an automatic perfusion structure, so that the automatic perfusion apparatus can be conveniently intelligently operated such as automatically adding medicine and changing liquid, and the flow rate of the liquid can be adjusted.
The working principle is as follows: when the device is used, a vessel filled with living cells is placed on the transparent glass 5 of the observation platform 1, the monitoring head 7 in the support rod 2 is used for monitoring the living cells in the vessel, the temperature detection 8 moves downwards under the action of the first air cylinder 17 in the living cell culture process until the temperature changes close to the vessel, and the concentration of O2 can be accurately controlled by the arranged accurate oxygen probe 16, so that the situation that the in-vivo gas environment cannot be truly simulated by in-vitro cultured cells is solved, the humidity changes close to the vessel under the action of the humidity detection 10 by the second air cylinder 18 in the support rod 2 are provided with favorable evidences for the data of cell changes, and the scanning and recording can be carried out by the fluorescent signal imaging device 16 below the transparent glass 5 at each cell culture forming stage, the fluorescent signal imaging device 16 can move back and forth along the chute 14 under the driving of the movable rod 15 to scan and observe the migration process of cells in real time, so that research data is more reliable, when medicine needs to be added or liquid needs to be changed, the medicine can enter the water tank 27 through the water inlet pipe 31 in the support rod 2, the medicine is fed into the water pipe between the water tank 27 and the second water pump 35 through the first water pump 33 of the water tank 30 in the water tank 27 as shown in fig. 4 until the medicine reaches the sewer pipe 36, meanwhile, the water tank 27 is pushed to the upper side of the culture dish by the third air cylinder 29 under the action of the control equipment, and the movable plate 36 is pushed downwards by the fourth air cylinder 34 to enable the sewer pipe 36 to be close to the culture dish, so that intelligent operations such as automatic medicine adding and liquid changing can be completed, and the flow rate of the liquid can be adjusted.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (6)
1. A living cell hypoxia culture imaging system instrument, includes observation platform (1), its characterized in that: the utility model discloses an inspection table, including observation platform (1), bracing piece (2), first recess (4) have been seted up to observation platform (1) rear end, be provided with clear glass (5) in first recess (4), second recess (13) have been seted up in observation platform (1), be provided with fluorescence signal imaging device (16) in second recess (13), be provided with first treater (11) and first controller (12) in observation platform (1), bracing piece (2) lower extreme is provided with monitoring head (7), bracing piece (2) lower extreme is provided with temperature detection (8), bracing piece (2) inner wall is provided with humidity detection (10), bracing piece (2) lower extreme swing joint has movable cover (25).
2. A living cell hypoxic culture imaging system apparatus according to claim 1, wherein: bracing piece (2) lower extreme is equipped with accurate oxygen probe (26), spout (14), two have all been seted up to second recess (13) inner wall both sides all be equipped with movable rod (15) between spout (14), observation platform (1) front end is provided with switch (6), bracing piece (2) inner wall fixedly connected with saddle (9), be provided with second cylinder (18) in saddle (9), second cylinder (18) and humidity are surveyed (10) swing joint.
3. A living cell hypoxic culture imaging system apparatus according to claim 1, wherein: a first air cylinder (17) is arranged in the supporting rod (2), and the lower end of the first air cylinder (17) is movably connected with the temperature detection device (8).
4. A living cell hypoxic culture imaging system apparatus according to claim 2, wherein: the movable rod (15) is internally provided with a second controller (19) and a second processor (20).
5. The apparatus of claim 1, wherein the apparatus comprises: symmetrical third grooves (21) are formed in the observation platform (1), the two third grooves (21) are communicated with the sliding groove (14), screw rods (22) are arranged in the two third grooves (21), sliding blocks (23) are in threaded connection in the two screw rods (22), and driving motors (24) are arranged at the front ends of the two screw rods (22).
6. A living cell hypoxic culture imaging system apparatus according to claim 1, wherein: the utility model discloses a water inlet, including bracing piece (2), water tank (27), swing joint has fly leaf (28) in water tank (27), be provided with third cylinder (29) in bracing piece (2), be equipped with basin (30) in water tank (27), be provided with intake pipe (31) in bracing piece (2), water inlet (32) have been seted up to water tank (27) one side, be equipped with first water pump (33) in basin (30), be equipped with fourth cylinder (34) in bracing piece (2), be provided with second water pump (35) in fly leaf (28), second water pump (35) lower extreme and fly leaf (28) swing joint, downcomer (36) are installed to fly leaf (28) lower extreme.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220717085.5U CN217948144U (en) | 2022-03-30 | 2022-03-30 | Imaging system instrument is cultivateed to living cell hypoxemia |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220717085.5U CN217948144U (en) | 2022-03-30 | 2022-03-30 | Imaging system instrument is cultivateed to living cell hypoxemia |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217948144U true CN217948144U (en) | 2022-12-02 |
Family
ID=84209549
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220717085.5U Expired - Fee Related CN217948144U (en) | 2022-03-30 | 2022-03-30 | Imaging system instrument is cultivateed to living cell hypoxemia |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217948144U (en) |
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2022
- 2022-03-30 CN CN202220717085.5U patent/CN217948144U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20221202 |