CN217948143U - Flow type electroporation modularization device - Google Patents

Abstract

The utility model discloses a stream type electroporation modularization device for carry out the electroporation to the cell suspension and handle. The modularized device comprises a base and an electroporation component, wherein the base is provided with an electric connection port for electrically connecting with an external circuit, the base is provided with an inner cavity, the base is provided with an inlet and an outlet, and the inlet and the outlet are respectively communicated with the inner cavity; the electroporation component comprises at least one group of electrode components, the electrode components are fixedly arranged in the inner cavity, and the electroporation component is connected with the outside through an inlet and an outlet. The utility model provides a STREAMING electroporation modularization device, with disposable consumptive material integration such as required electroporation subassembly in the STREAMING electroporation treatment on the base, the design of base has both protected the electroporation subassembly not receive the pollution, also provides the passageway for electroporation subassembly and external connection, and can contain multiunit electrode subassembly simultaneously, has improved the cell handling capacity of every set of consumptive material, reduce cost, the operator of being convenient for simultaneously installs and uses.

Description

Flow type electroporation modularization device

Technical Field

The utility model relates to biotechnology especially relates to a STREAMING electroporation modularization device.

Background

Electroporation (Electroporation) is a technique that uses an electric field to act on a cell membrane to make the cell membrane generate micropores through which exogenous molecules such as DNA, RNA, and protein can pass, and these micropores are generated instantaneously under the action of the electric field and can recover with the disappearance of the electric field, thereby avoiding permanent damage to the cell. Electroporation, which is known as Electrotransfection technology (Electrotransfection), can introduce a variety of foreign molecules such as DNA, RNA, proteins, carbohydrates, dyes, viral particles, etc. into prokaryotic or eukaryotic cells; electrotransfection technology is widely applied in many high-end biological medicine technical fields such as antibody protein production, in Vitro Diagnosis (IVD) reagent raw material production, gene editing and gene modification of immune cells and the like, and is an important technology for delivering substances in cells.

The methods for electroporation of cells are various, among which, the flow electroporation technique can perform continuous electroporation of flowing cell-containing suspension (hereinafter referred to as cell suspension) with high efficiency, and is particularly suitable for batch processing of large-volume cell suspensions. A conventional flow electroporation module mainly includes a pair of planar electrodes for generating an electric field, and a chamber located between the two planar electrodes, and the cell suspension flows through the chamber while receiving an electric shock to generate electroporation, so that exogenous molecules in the cell suspension can enter target cells.

Generally, to ensure that the cell suspension is not contaminated, the whole electroporation process needs to be performed in a strict sterile environment, so that the storage container, the transmission pipeline, the planar electrode, and the like in direct contact with the cell suspension in each processing batch are disposable consumables and are not reusable. In the prior art, a batch of brand-new storage containers, transmission pipelines, electrode assemblies and other parts are generally connected and packaged one by one, and a disposable consumable bag is formed after sterilization. Each consumable pack contains an electrode assembly that has a limited working time and limited cell fluids that can be electroporated, thus requiring multiple consumables to be used if a larger volume of cell fluid needs to be processed. The time for replacing the consumable is also consumed, the cell recovery rate is reduced due to the residual cell sap, and the cost of electroporation is increased.

Therefore, it is necessary to provide a flow electroporation treatment apparatus with high electrotransfection efficiency and cell survival rate, large and adjustable cell treatment capacity, no risk of cell contamination, convenient use and low cost.

SUMMERY OF THE UTILITY MODEL

The utility model aims at the not enough that prior art exists, provide one kind can contain multiunit electrode subassembly, cell handling capacity great and adjustable, and electrotransfection efficiency and cell survival rate all relatively high STREAMING electroporation modularization device.

In order to achieve the above purpose, the utility model adopts the technical scheme that:

a flow electroporation modular device for electroporation treatment of cell suspension, the modular device comprising a base and an electroporation component, the base having an electrical connection port for electrical connection with an external circuit, the base having an inner cavity, the base having an inlet and an outlet, the inlet and the outlet being respectively in communication with the inner cavity; the electroporation component comprises at least one group of electrode components, the electrode components are fixedly arranged in the inner cavity, and the electroporation component is connected with the outside through the inlet and the outlet.

In some embodiments, a mounting seat is arranged in the inner cavity, the mounting seat is provided with a mounting groove, the mounting groove comprises at least one electrode groove, the number of the electrode grooves is not less than that of the electrode assemblies, and each group of the electrode assemblies can be arranged in one of the electrode grooves in a matched mode.

In some embodiments, the mounting groove further comprises a plurality of connecting pipe grooves, the electrode grooves are arranged at intervals, the connecting pipe grooves are provided with one or more connecting pipes, and different electrode grooves are communicated with each other through the connecting pipe grooves.

In some embodiments, the electrode assemblies have multiple sets, and the electroporation assembly further comprises one or more connecting lines, through which different electrode assemblies are connected, each connecting line being fittingly disposed in at least one of the connecting tube slots.

In some embodiments, the electrode grooves are arranged at intervals along the length direction of the base, and every two adjacent electrode grooves are communicated with each other through one connecting pipe groove along the length direction of the base.

In some embodiments, the electrode assemblies have a plurality of groups, the electroporation assembly further includes a connecting line, two adjacent groups of the electrode assemblies are connected by one connecting line, the connecting line is located between the two groups of the electrode assemblies along the length direction of the base, the two groups of the electrode assemblies are respectively disposed in the two electrode slots, the connecting line is disposed in the connecting pipe slot between the two electrode slots, or the connecting line is disposed in a plurality of the connecting pipe slots and at least one of the electrode slots.

In some embodiments, each of the electrode slots has a first notch and a second notch respectively arranged on two sides of the base in the width direction, and the first notches of all the electrode slots are located on the same side of the corresponding second notch along the width direction of the base; in every two adjacent electrode tanks along the length direction of the base, the connecting pipe tank is connected between the first notch of the electrode tank positioned on the front side and the second notch of the electrode tank positioned on the rear side.

In some embodiments, the mounting groove further includes a liquid inlet pipe groove and a liquid outlet pipe groove, the electrode grooves located at two different sides of the mounting seat along the length direction of the base are respectively a first electrode groove and a second electrode groove, one end of the liquid inlet pipe groove is communicated with the first notch of the first electrode groove, and the other end of the liquid inlet pipe groove is communicated with the inlet; one end of the liquid outlet pipe groove is communicated with the second notch of the second electrode groove, and the other end of the liquid outlet pipe groove is communicated with the outlet.

In some embodiments, each of the connecting pipe slots has a first turning section, a second turning section and a third turning section which are sequentially connected along the length direction of the connecting pipe slot, and the first turning section and the third turning section are located on the same side of the second turning section along the length direction of the base.

In some embodiments, the axes of all the connecting pipe slots are straight lines or smooth arc lines, and the axes of all the connecting pipe slots and the axes of all the electrode slots extend in the same line.

In some embodiments, along the length extending direction of the mounting groove, one or more sets of positioning protrusions are arranged in the mounting groove, each set of positioning protrusions comprises two positioning protrusions which are respectively arranged on the inner walls of two sides of the mounting groove, and the two positioning protrusions are intersected with the same cross section of the mounting groove.

In some embodiments, the inlet and the outlet are respectively arranged at two different sides of the base in the width direction, and the axial line of the inlet and the axial line of the outlet extend in parallel or collinearly; along the length direction of base, the electricity connection port is located one side of base, the entry with the export is all located the opposite side of base.

In some embodiments, the electrode assembly includes two planar electrodes disposed at an interval, an electroporation chamber is formed between the two planar electrodes for cell suspension to flow through, at least one pair of conductive members is disposed in the inner cavity, each pair of conductive members includes a positive conductive member and a negative conductive member, the positive conductive member and the negative conductive member are respectively electrically connected to the electrical connection port, the positive conductive member and the negative conductive member are both made of elastic conductive material, a clamping groove is formed between the positive conductive member and the negative conductive member, each group of the electrode assemblies is disposed in one of the clamping grooves, and the positive conductive member and the negative conductive member respectively abut against the planar electrode on a corresponding side of the electrode assembly.

In some embodiments, the electroporation component further comprises a liquid inlet pipe and a liquid outlet pipe, the liquid inlet pipe and the liquid outlet pipe are respectively connected with the electrode component, the liquid inlet pipe extends out of the base through the inlet, and the liquid outlet pipe extends out of the base through the outlet.

Because above technical scheme's application, the utility model provides a STREAMING electroporation modularization device, with disposable consumptive material integration such as required electroporation subassembly among the STREAMING electroporation treatment on the base, the design of base has both protected the electroporation subassembly not receive the pollution, also provides the passageway for electroporation subassembly and external connection, the operator of being convenient for simultaneously installs, uses and dismantles. The base can be directly electrically connected with external equipment, and then an electrode assembly inside the base is powered to carry out electroporation treatment. The external shape of the modular device is determined by the shape of the base, which the manufacturer can design into various specifications of geometry as required to match with different external devices, and preferably to make the base into a flat, compact box. The structure of the electroporation component in the base can be different specifications, the appearance of the base can be modularized and standardized, the production cost is reduced, the use method of an operator is more convenient, the operation flow is more standard, and the error is not easy to occur.

Drawings

In order to more clearly illustrate the technical solution of the present invention, the drawings used in the description of the embodiments will be briefly described below.

FIG. 1 is a schematic perspective view of a modular apparatus of example 1;

FIG. 2 is an exploded perspective view of the modular apparatus of example 1;

FIG. 3 is a schematic perspective view of an electroporation component of example 1;

FIG. 4 is a perspective view of a mount in embodiment 1;

fig. 5 is a schematic perspective view of a circuit board in embodiment 1;

FIG. 6 is a side view of the modular apparatus of example 1 with the first housing removed;

FIG. 7 is a schematic side view of a partial connecting tube groove in example 1;

FIG. 8 is a schematic side view of an electroporation component of example 1;

FIG. 9 is a schematic side view of an electroporation component of example 2;

FIG. 10 is a schematic side view of an electroporation component of example 3;

FIG. 11 is a schematic side view of an electroporation component of example 4;

FIG. 12 is a schematic side view of the modular apparatus of example 5 with the first housing removed;

FIG. 13 is a schematic side view of an electroporation component of example 6;

FIG. 14 is a schematic side view of the electroporation component of example 7;

FIG. 15 is a schematic side view of an electroporation component of example 8;

FIG. 16 is a schematic side view of an electroporation component of example 9;

wherein: 100. a base; 101. an inlet; 102. an outlet; 103. an electrical connection port; 110. a first housing; 120. a second housing; 130. a mounting seat; 131. an electrode tank; 1311. a first electrode tank; 1312. a second electrode tank; 131a, a first notch; 131b, a second notch; 132. connecting a pipe groove; 132a, a first revolution segment; 132b, a second revolution segment; 132c, a third revolution segment; 1321. a liquid inlet pipe groove; 1322. a liquid outlet pipe groove; 133. positioning the projection; 140. a circuit board; 141. a conductive member; 1411. a positive electrode conductive member; 1412. a negative electrode conductive member; 142. a clamping groove;

200. an electroporation component; 210. an electrode assembly; 211. a planar electrode; 213. an electrode inlet; 214. an electrode outlet; 220. Connecting a pipeline; 221. a liquid inlet pipe; 222. a liquid outlet pipe.

Detailed Description

The following detailed description of the preferred embodiments of the invention, taken in conjunction with the accompanying drawings, enables the advantages and features of the invention to be more readily understood by those skilled in the art.

Example 1

Referring to fig. 1 and 2, a flow electroporation modular apparatus for electroporation of a cell suspension is shown. The modular apparatus comprises a base 100 and an electroporation component 200, wherein the base 100 has an electrical connection port 103 for electrically connecting with an external circuit (not shown), the base 100 has an inner cavity, the base 100 has an inlet 101 and an outlet 102, and the inlet 101 and the outlet 102 are respectively communicated with the inner cavity. The electroporation component 200 includes at least one set of electrode components 210, the electrode components 210 are fixed in the inner cavity, and the electroporation component 200 is connected with the outside through the inlet 101 and the outlet 102.

Referring to fig. 1, in the present embodiment, an XYZ three-dimensional coordinate system is established with reference to a base 100, wherein the base 100 is perpendicular to each other in a length direction X, a width direction Y, and a thickness direction Z. When the base 100 is placed in the orientation shown in fig. 1, the width direction Y extends along the vertical direction, the inlet 101 and the outlet 102 are respectively disposed at two different sides of the width direction Y of the base 100, wherein the inlet 101 faces downward and the outlet 102 faces upward; the length direction X and the thickness direction Z extend along the horizontal direction, respectively, and along the length direction X, the electrical connection port 103 is disposed at the rear side of the base 100, and the inlet 101 and the outlet 102 are both disposed at the front side of the base 100; this state is the orientation of the modular apparatus of this embodiment when the electroporation process is normally performed. The modular device may be placed in any other orientation, in other states of use or not. Therefore, the XYZ three-dimensional coordinate system has no specific orientation in an absolute space, and the orientations such as "up and down", "front and back", "left and right" referred to herein are defined based on the orientation shown in fig. 1, and are only for convenience of description and understanding, and should not be construed as limiting the present invention.

In the present embodiment, the axial line of the inlet 101 and the axial line of the outlet 102 extend parallel to or collinear with each other, and here extend collinear in the width direction Y of the base 100. When the modular device is used, the electrical connection port 103 can be correspondingly connected with an external circuit, specifically, the electrical connection port 103 can be fixedly inserted into a certain power supply device, and at this time, the inlet 101 and the outlet 102 are relatively far away from the electrical connection port 103, so that the power supply device cannot interfere with the connection between the electroporation component 200 and an external pipeline.

Referring to FIG. 2, in the present embodiment, the electrode assemblies 210 have multiple sets, and each set of electrode assemblies 210 has an electroporation chamber for flowing a cell suspension. The electroporation component 200 further comprises one or more connecting lines 220, wherein different electrode components 210 are connected by the connecting lines 220, and specifically, the electroporation chamber of each group of electrode components 210 is communicated with the connecting lines 220. In this embodiment, the electrode assemblies 210 have three sets connected in series, and each two adjacent sets of electrode assemblies 210 are connected by a connecting pipe 220. The electroporation component 200 further comprises a liquid inlet pipe 221 and a liquid outlet pipe 222, wherein the liquid inlet pipe 221 and the liquid outlet pipe 222 are respectively connected with the electrode assemblies 210, and are respectively connected with different electrode assemblies 210, the liquid inlet pipe 221 extends out of the base 100 through the inlet 101, and the liquid outlet pipe 222 extends out of the base 100 through the outlet 102, so that the cell suspension can flow from the liquid inlet pipe 221 through the electroporation chambers of the electrode assemblies 210, and then flow out of the liquid outlet pipe 222 after the electroporation process. That is, in this embodiment, the inlet 101 and the outlet 102 only serve as a through channel for the liquid inlet pipe 221 and the liquid outlet pipe 222, and the cell suspension does not directly flow through the inlet 101 and the outlet 102. Liquid inlet pipe 221 may further be connected to a liquid inlet container, and liquid outlet pipe 222 may be connected to a collection container (neither shown).

Referring to fig. 2, in the present embodiment, the base 100 includes a first housing 110, a second housing 120, a mounting base 130 and a circuit board 140 that are fixed to each other. The first casing 110 and the second casing 120 are respectively disposed on two sides of the thickness direction Z, an inner cavity is defined between the first casing 110 and the second casing 120, the mounting base 130 and the circuit board 140 are both fixedly disposed in the inner cavity, the mounting base 130 has a mounting groove into which the electroporation component 200 is fittingly inserted, and the mounting base 130 and the circuit board 140 jointly implement mounting and positioning of the electroporation component 200. In this embodiment, the first housing 110, the second housing 120 and the mounting base 130 are made of plastic by injection molding, and the cost is low, and the whole modular device including the base 100 and the electroporation component 200 is a disposable consumable material, so that the pollution risk caused by repeated use can be avoided, and the effect of the whole electrotransfection treatment can be improved.

Referring to fig. 3 and 8, in the present embodiment, the three groups of electrode assemblies 210 have the same structure, each group of electrode assemblies 210 includes two planar electrodes 211 arranged at intervals, the electroporation chamber is formed between the two planar electrodes 211, and after the two planar electrodes 211 are electrified, an electric field can be formed in the corresponding electroporation chamber, so as to perform electroporation on the cell suspension flowing through the electroporation chamber. Specifically, in each set of electrode assemblies 210, two planar electrodes 211 are disposed at intervals along the length direction X of the base 100, the electroporation chamber extends along the width direction Y of the base 100, the upper and lower ends of the electroporation chamber respectively have an electrode inlet 213 and an electrode outlet 214, and the electrode inlet 213 is located below the electrode outlet 214. When the cell suspension flows through the electroporation chambers, the cell suspension flows from the electrode inlets 213 to the corresponding electrode outlets 214, that is, the cell suspension flows in a downward-in-upward-out direction in each electroporation chamber, so that the part of the electroporation chamber through which the cell suspension flows can be filled with the liquid all the time, and bubbles generated by water electrolysis in the electroporation process can be reduced, and the cell suspension can continuously and uniformly flow through the electroporation chamber, which is beneficial to improving the electroporation efficiency and the cell survival rate.

In this embodiment, three sets of electrode assemblies 210 are sequentially disposed along the length direction X of the base 100, and the connecting pipe 220 between each two adjacent electrode assemblies 210 is connected between the electrode outlet 214 of the electrode assembly 210 on the rear side and the electrode inlet 213 of the electrode assembly 210 on the front side. The connection pipe 220 is disposed between the adjacent two sets of electrode assemblies 210 along the length direction X of the base 100, and the liquid inlet pipe 221 is connected to the electrode inlet 213 of the electrode assembly 210 on the rearmost side, and the liquid outlet pipe 222 is connected to the electrode outlet 214 of the electrode assembly 210 on the foremost side. Thus, the three sets of electrode assemblies 210 are serially connected from back to front, and the cell suspension flowing from the liquid inlet pipe 221 can flow through the electroporation chambers of the three sets of electrode assemblies 210 from bottom to top, and finally flows out from the liquid outlet pipe 222.

Referring to fig. 4, in the present embodiment, the mounting groove includes a plurality of electrode grooves 131 and a plurality of connecting pipe grooves 132, wherein the electrode grooves 131 are arranged at intervals, the number of the electrode grooves 131 is not less than the number of the electrode assemblies 210, the connecting pipe grooves 132 include one or more electrode grooves 132, different electrode grooves 131 are communicated with each other through the connecting pipe grooves 132, each group of electrode assemblies 210 can be fittingly arranged in one of the electrode grooves 131, and each connecting pipe 220 can be fittingly arranged in at least one of the connecting pipe grooves 132. In this embodiment, each of the plurality of electrode slots 131 is a through slot penetrating through the mounting base 130 in the thickness direction Z of the base 100, and each of the connecting tube slots 132 is a blind slot opened at one side of the mounting base 130, where an opening of the connecting tube slot 132 faces the first housing 110 in the thickness direction Z. Specifically, a plurality of electrode slots 131 are provided at intervals along the length direction X of the susceptor 100; along the length direction X of the base 100, every two adjacent electrode slots 131 are communicated with each other through a connecting tube slot 132. In this embodiment, three electrode slots 131 are specifically provided, and two connecting tube slots 132 are provided between the three electrode slots 131. In addition, the mounting grooves further include a liquid inlet pipe groove 1321 and a liquid outlet pipe groove 1322, at least a portion of the liquid inlet pipe 221 can be fittingly mounted in the liquid inlet pipe groove 1321, and at least a portion of the liquid outlet pipe 222 can be fittingly mounted in the liquid outlet pipe groove 1322.

Referring to fig. 4, each electrode slot 131 has a first notch 131a and a second notch 131b respectively disposed on two sides of the base 100 in the width direction Y. The first notch 131a of all the electrode grooves 131 is located on the same side of the corresponding second notch 131b in the width direction Y of the base 100. In this embodiment, the first notch 131a is specifically located at the lower side of the second notch 131b, when a group of electrode assemblies 210 is correspondingly disposed in one of the electrode slots 131, the electrode inlet 213 of the electrode assembly 210 is disposed in the first notch 131a, and the electrode outlet 214 is disposed in the second notch 131b. In every adjacent two electrode tanks 131 in the length direction X of the susceptor 100, the connection pipe tank 132 is connected between the first notch 131a of the electrode tank 131 located at the front side and the second notch 131b of the electrode tank 131 located at the rear side.

Referring to fig. 4, in the present embodiment, the electrode grooves 131 located at two different sides of the mounting seat 130 along the length direction X of the base 100 are a first electrode groove 1311 and a second electrode groove 1312, where the first electrode groove 1311 is located at the rear side and the second electrode groove 1312 is located at the front side. One end of the liquid inlet pipe slot 1321 is communicated with the first notch 131a of the first electrode slot 1311, and the other end is communicated with the inlet 101; one end of the liquid outlet pipe 1322 is communicated with the second notch 131b of the second electrode tank 1312, and the other end is communicated with the outlet 102. Further, since the inlet 101 and the outlet 102 are disposed at the front side of the base 100, i.e., at the side close to the second electrode groove 1312, in this embodiment, the liquid outlet pipe 1322 is disposed to extend upward along the width direction Y of the base 100, the axial line of the inlet 101 and the axial line of the outlet 102 both pass through the second electrode groove 1312, and the liquid inlet pipe 1321 is bent from the back to the front and then extends to the lower side of the second electrode groove 1312.

In other embodiments, the three electrode slots 131 may be sequentially connected in series from front to back along the length direction X of the base 100, one end of the liquid inlet pipe slot 1321 is communicated with the first slot 131a of the frontmost electrode slot 131, and one end of the liquid outlet pipe slot 1322 is communicated with the second slot 131b of the rearmost electrode slot 131.

Referring to fig. 4, 6 and 7, in the present embodiment, each of the connecting pipe slots 132 between the two electrode slots 131 has a first turning section 132a, a second turning section 132b and a third turning section 132c sequentially connected along the length direction thereof. The first and third turning sections 132a and 132c are located on the same side of the second turning section 132b along the length direction X of the base 100. Further, as shown in fig. 7, each of the connecting pipe slots 132 has two upper and lower sets of a first turning section 132a, a second turning section 132b and a third turning section 132c (in the figure, the third turning section 132c located at the upper side and the first turning section 132a located at the lower side actually refer to the same section), so that the connecting pipe slot 132 extends in an "S" shape as a whole, when the connecting pipe 220 is fittingly installed in the connecting pipe slot 132, the connecting pipe 220 can extend in the "S" shape in approximately the same shape, and thus the connecting pipe 220 has a smaller curvature, and is not easy to bend to cause obstruction or even blockage of cell suspension flow.

Referring to fig. 6, in the present embodiment, three sets of electrode assemblies 210 are disposed in one-to-one correspondence in three electrode slots 131, and the connecting pipe 220 between each two adjacent electrode assemblies 210 is disposed in one connecting pipe slot 132 between the two corresponding electrode slots 131 along the length direction X of the base 100.

In this embodiment, along the length extending direction of mounting groove, all be equipped with a set of or multiunit location arch 133 in every mounting groove, every group location arch 133 is including dividing two of locating the both sides inner wall of mounting groove, and two location archs 133 are crossing with the same cross section of mounting groove to can form spacing and fixed to the electroporation subassembly 200 that sets up in the mounting groove. Specifically, in this embodiment, the positioning protrusion 133 is disposed on the inner side wall of the connecting pipe groove 132 and the liquid inlet pipe groove 1321. When the cooperation of electricity perforation subassembly 200 was installed in the mounting groove, just can extend and arrange with the direction that the mounting groove set for, the mounting groove plays fixed and spacing effect. In this embodiment, the axial lines of the inlet pipe slot 1321, the outlet pipe slot 1322 and all the connecting pipe slots 132 are straight lines or smooth arc lines. The axial lines of the liquid inlet pipe 1321, the liquid outlet pipe 1322 and all the connecting pipe grooves 132 extend in the same line with the axial lines of all the electrode grooves 131, and are all located in the same virtual plane, which is a plane parallel to the length direction X and the width direction Y of the base 100, so that the flow of the cell suspension in the electroporation component 200 does not have violent change or blockage of the flow speed due to the sudden change of the corner in the Z direction, and the flow of the cell suspension is smoother and more uniform.

Referring to fig. 5, in the present embodiment, at least one pair of conductive elements 141 is disposed in the inner cavity, each pair of conductive elements 141 includes a positive conductive element 1411 and a negative conductive element 1412, and the positive conductive element 1411 and the negative conductive element 1412 are electrically connected to the electrical connection port 103, respectively. Positive electrode conductive element 1411 and negative electrode conductive element 1412 are electrically connected to one planar electrode 211 of one set of electrode assemblies 210, respectively. In this embodiment, a clamping groove 142 is formed between each pair of conductive members 141, and when a set of electrode assemblies 210 is disposed in the clamping groove 142, the positive conductive member 1411 and the negative conductive member 1412 respectively abut against the planar electrodes 211 on the corresponding sides of the electrode assemblies 210. The positive conductive element 1411 and the negative conductive element 1412 are made of elastic conductive materials, and may be metal elastic pieces such as bent copper sheets, so as to be closely attached to the outer side surface of the planar electrode 211, and have more stable contact and larger conductive area. In this embodiment, the pairs of conductive members 141 are fixed on one side of the circuit board 140 and electrically connected to the electrical connection port 103 through a circuit on the circuit board 140. The clamping grooves 142 have three corresponding to the three electrode grooves 131, the three clamping grooves 142 are arranged at intervals along the length direction X of the base 100 and correspond to the positions of the three electrode grooves 131 one by one, and each pair of the conductive members 141 can be inserted into the corresponding electrode groove 131. Each of the clamping grooves 142 has four conductive members 141 on both sides in the length direction X, and each of the four conductive members 141 has two positive conductive members 1411 or two negative conductive members 1412, so that the four conductive members 141 together stably clamp and supply power to a group of the electrode assemblies 210. When the modular device is in operation, a control system in the power supply equipment can determine whether the electrode assembly 210 is disposed in each clamping groove 142, whether the conductive member 141 is closely mounted to the planar electrode 211, and whether the contact between the conductive member 141 and the planar electrode 211 is good by detecting a voltage difference between the conductive members 141 at both sides of each clamping groove 142. The power supply device transmits the high-voltage pulse signal to each conductive member 141 through the electrical connection port 103 and the circuit board 140, and further transmits the high-voltage pulse signal to each planar electrode 211, so that the control system can control an electroporation processing program in the modular device by controlling the start-stop, the intensity, the frequency and the like of the pulse signal, and perform electroporation processing in different modes as required.

Example 2

Referring to fig. 9, the present embodiment provides a flow electroporation modular apparatus comprising a base 100 and an electroporation component 200. The structure of the base 100 is the same as that of embodiment 1, and the structure of the electroporation component 200 is substantially the same as that of embodiment 1, the main difference being that the electroporation component 200 in this embodiment includes two sets of electrode assemblies 210 connected in series.

In this embodiment, the mounting seat 130 is also provided with three identical electrode grooves 131, which are sequentially called as a second electrode groove 1312, a middle electrode groove 131 and a first electrode groove 1311 from front to back along the length direction X of the base 100. The two sets of electrode assemblies 210 of the electroporation assembly 200 are disposed in the first electrode bath 1311 and the second electrode bath 1312, respectively, and the electrode assembly 210 is not disposed in the middle electrode bath 131. In this embodiment, the length of the connecting pipe 220 between the two sets of electrode assemblies 210 is longer than that in embodiment 1, and the connecting pipe 220 is simultaneously disposed in the plurality of connecting pipe grooves 132 and at least one electrode groove 131, specifically, the middle electrode groove 131 and the two connecting pipe grooves 132 on the front and rear sides thereof. Due to the guidance and limitation of the installation slot, the connecting line 220 in this embodiment is shown in a curved configuration.

In this embodiment, since the electrode assembly 210 is not disposed in the middle electrode tank 131, the planar electrode 211 is not electrically connected to the conductive members 141 on both sides of the middle holding tank 142, and the control system can adjust the program accordingly after detecting that there is no electrode assembly 210 in the holding tank 142, and no voltage is applied to the pair of conductive members 141.

It is understood that the structure of the electroporation component 200 may be different based on the same base 100. The mounting base 130 of the present embodiment can accommodate at most three electrode assemblies 210 connected in series, and the mounting base 130 can accommodate only two electrode assemblies 210 connected in series or only one electrode assembly 210 according to actual needs. When the number of the electrode assemblies 210 in the electroporation assembly 200 is less than three, the electrode assemblies 210 may be disposed in any two or one of the electrode baths 131, and the liquid inlet pipe 221 and/or the liquid outlet pipe 222 and/or the connecting pipe 220 connected thereto may be disposed in the other electrode baths 131, the connecting pipe bath 132, the liquid inlet pipe bath 1321 and the liquid outlet pipe bath 1322, respectively. From the appearance, the modular device having the electroporation component 200 with different structures is not different in appearance, and the using method is the same. Therefore, the modularized device provided by the embodiment is not only beneficial to batch production, but also capable of standardizing the operation flow of an operator and reducing the occurrence of human errors.

Example 3

Referring to fig. 10, the present embodiment provides a flow electroporation modular apparatus comprising a base 100 and an electroporation component 200. The structure of the base 100 is the same as that of embodiment 1, and the structure of the electroporation component 200 is substantially the same as that of embodiment 1, the main difference being that the electroporation component 200 in this embodiment includes two sets of electrode assemblies 210 connected in series.

In this embodiment, the mounting seat 130 is also provided with three identical electrode grooves 131, which are sequentially called as a second electrode groove 1312, a middle electrode groove 131 and a first electrode groove 1311 from front to back along the length direction X of the base 100. The two sets of electrode assemblies 210 of the electroporation component 200 are respectively disposed in the middle electrode tank 131 and the second electrode tank 1312, the electrode assembly 210 is not disposed in the first electrode tank 1311, and the power supply device does not apply voltage to the conductive member 141 in the first electrode tank 1311. In this embodiment, the length of the liquid inlet pipe 221 located in the inner cavity is longer than that in embodiment 1, and the liquid inlet pipe 221 is simultaneously disposed in the liquid inlet pipe slot 1321, the first electrode slot 1311, and the connecting pipe slot 132 in front of the first electrode slot 1311. The appearance of the modular device in this embodiment is also exactly the same as that of embodiment 1, which facilitates modular production and use.

Example 4

Referring to fig. 11, the present embodiment provides a flow electroporation modular apparatus comprising a base 100 and an electroporation component 200. The structure of the base 100 is the same as that of embodiment 1, and the structure of the electroporation component 200 is substantially the same as that of embodiment 1, the main difference being that the electroporation component 200 in this embodiment includes only one set of electrode components 210. In this embodiment, the electroporation component 200 does not include the connecting pipe 220, the electrode inlet 213 of the electrode component 210 is directly connected to the liquid inlet pipe 221, and the electrode outlet 214 of the electrode component 210 is directly connected to the liquid outlet pipe 222.

In this embodiment, three identical electrode slots 131 are also formed in the mounting base 130 along the length direction X of the base 100From front to back in turn calledA second electrode tank 1312, an intermediate electrode tank 131, and a first electrode tank 1311. In this embodiment, the electrode assembly 210 is disposed in the second electrode groove 1312, part of the liquid outlet pipe 222 is disposed in the liquid outlet pipe groove 1322, and the length of the liquid inlet pipe 221 in the inner cavity is longer than that in embodiment 3. In this embodiment, the liquid inlet pipe 221 is sequentially disposed in the front connection pipe 132, the middle electrode tank 131, the rear connection pipe 132, the first electrode tank 1311, and the liquid inlet pipe 1321 from front to rear, and extends out of the susceptor 100 through the inlet 101. The appearance of the modular device in this embodiment is also exactly the same as that of embodiment 1, which facilitates modular production and use.

Example 5

Referring to fig. 12, the present embodiment provides a flow electroporation modular apparatus comprising a base 100 and an electroporation component 200. The structure of the base 100 and the electroporation component 200 are substantially the same as that of embodiment 4, and the electroporation component 200 includes only one set of electrode assemblies 210, mainly different from the way in which the electroporation component 200 is installed in the base 100.

In this embodiment, the first notch 131a of the second electrode tank 1312 is directly communicated with the inlet 101 directly below it. Thus, the liquid inlet pipe 221 below the electrode assembly 210 can directly extend out of the base 100 through the inlet 101 downward along the width direction Y of the base 100, and the liquid inlet pipe 221 does not need to be disposed in the liquid inlet pipe slot 1321 and other mounting slots, so that the mounting manner is simpler. The appearance of the modular device in this embodiment is also exactly the same as that of embodiment 1, which facilitates modular production and use.

Example 6

Referring to fig. 13, the present embodiment provides a flow electroporation modular apparatus comprising a base 100 and an electroporation component 200. The structure of the base 100 is substantially the same as that of embodiment 1, and the main difference is that only two electrode slots 131 connected in series are provided on the mounting base 130, and the number of the connecting tube slots 132 is reduced accordingly. In the present embodiment, the axis of the inlet 101 extends in line with the axis of the outlet 102 and is located on the front side of the base 100. The electroporation assembly 200 includes two sets of electrode assemblies 210 connected in series, the two sets of electrode assemblies 210 are respectively and correspondingly disposed in the two electrode tanks 131, and the connecting pipes 220 between the two sets of electrode assemblies 210 are correspondingly disposed in the middle connecting pipe tank 132. In this embodiment, the length of the base 100 is slightly shorter than that of embodiment 1, and the size of the modular device is smaller.

Example 7

Referring to fig. 14, the present embodiment provides a flow electroporation modular apparatus comprising a base 100 and an electroporation component 200. The structure of the base 100 is substantially the same as that of embodiment 6, and the main difference is that the axial line of the inlet 101 is parallel to but not collinear with the axial line of the outlet 102, the inlet 101 is disposed at the rear side of the base 100, and the outlet 102 is disposed at the front side of the base 100, so that the inlet pipe slot 1321 and the outlet pipe slot 1322 extend downward and upward along the width direction Y of the base 100, respectively. In this embodiment, the structure of the electroporation section 200 is the same as that of embodiment 4, and the extending direction of the liquid inlet pipe 221 is changed by changing the shape of the liquid inlet pipe slot 1321.

Example 8

Referring to fig. 15, the present embodiment provides a flow electroporation modular apparatus comprising a base 100 and an electroporation component 200. The overall structure of the base 100 is substantially the same as that of embodiment 1, and includes a first housing 110, a second housing 120, a mounting base 130, a circuit board 140, and the like. The main difference is the structure of the electroporation component 200 and the mounting groove.

In this embodiment, the electroporation component 200 includes three sets of electrode assemblies 210, the three sets of electrode assemblies 210 are connected in parallel, the connecting pipeline 220 is correspondingly in a multi-branch structure, and the cell suspension flowing in from the liquid inlet pipe 221 is processed by electroporation of different electrode assemblies 210, and then is converged into the liquid outlet pipe 222 and flows out.

Accordingly, the shape of the mounting slot, and in particular the connecting tube slot 132 in this embodiment, is adjusted accordingly to the shape of the connecting tube 220 as shown, so that the mounting slot can guide and position the electroporation component 200.

Example 9

Referring to fig. 16, the present embodiment provides a flow electroporation modular apparatus comprising a base 100 and an electroporation component 200. The structure of the modular apparatus in this embodiment is substantially the same as that of embodiment 8, and the main difference is that the electroporation component 200 in this embodiment includes two sets of electrode components 210 connected in parallel, and the structure of the mounting groove is adjusted accordingly.

The above embodiments are only for illustrating the technical concept and features of the present invention, and the purpose of the embodiments is to enable people skilled in the art to understand the contents of the present invention and to implement the present invention, which cannot limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered by the protection scope of the present invention.

Claims (14)

1. A modular device for flow electroporation of a suspension of cells, comprising: the modularized device comprises a base and an electroporation component, wherein the base is provided with an electric connection port for electrically connecting with an external circuit, the base is provided with an inner cavity, the base is provided with an inlet and an outlet, and the inlet and the outlet are respectively communicated with the inner cavity; the electroporation component comprises at least one group of electrode components, the electrode components are fixedly arranged in the inner cavity, and the electroporation component is connected with the outside through the inlet and the outlet.

2. A flow electroporation modular apparatus as claimed in claim 1, wherein: the electrode assembly mounting structure is characterized in that a mounting seat is arranged in the inner cavity and provided with a mounting groove, the mounting groove comprises at least one electrode groove, the number of the electrode grooves is not less than that of the electrode assemblies, and each electrode assembly can be arranged in one of the electrode grooves in a matched mode.

3. A flow electroporation modular apparatus as claimed in claim 2, wherein: the mounting groove still includes the connecting tube groove, the electrode tank has a plurality ofly that the interval set up, the connecting tube groove has one or more, and is different pass through between the electrode tank the connecting tube groove is linked together.

4. A flow electroporation modular apparatus as claimed in claim 3, wherein: the electrode assemblies are provided with a plurality of groups, the electroporation assembly further comprises one or more connecting pipelines, different electrode assemblies are connected through the connecting pipelines, and each connecting pipeline can be arranged in at least one connecting pipe groove in a matched mode.

5. A flow electroporation modular apparatus as claimed in claim 3, wherein: the electrode grooves are arranged at intervals along the length direction of the base, and every two adjacent electrode grooves are communicated with each other through one connecting pipe groove along the length direction of the base.

6. A flow electroporation modular apparatus as claimed in claim 5, wherein: the electrode assemblies are provided with a plurality of groups, the electroporation component further comprises connecting pipelines, every two adjacent groups of electrode assemblies are connected through one connecting pipeline, the connecting pipelines are located between the two groups of electrode assemblies along the length direction of the base, the two groups of electrode assemblies are respectively arranged in the two electrode tanks, the connecting pipelines are arranged in the connecting pipe tanks between the two electrode tanks, or the connecting pipelines are simultaneously arranged in the connecting pipe tanks and at least one electrode tank.

7. A flow electroporation modular apparatus as claimed in claim 5, wherein: each electrode groove is provided with a first notch and a second notch which are respectively arranged on two sides of the base in the width direction, and the first notches of all the electrode grooves are positioned on the same side of the corresponding second notch along the width direction of the base; the length direction of base, every adjacent two among the electrode tank, the connecting tube groove is connected and is located the front side the electrode tank first notch with be located the rear side the electrode tank between the second notch.

8. A flow electroporation modular apparatus as claimed in claim 7, wherein: the mounting groove further comprises a liquid inlet pipe groove and a liquid outlet pipe groove, the electrode grooves positioned on two different sides of the mounting seat along the length direction of the base are respectively a first electrode groove and a second electrode groove, one end of the liquid inlet pipe groove is communicated with the first notch of the first electrode groove, and the other end of the liquid inlet pipe groove is communicated with the inlet; one end of the liquid outlet pipe groove is communicated with the second notch of the second electrode groove, and the other end of the liquid outlet pipe groove is communicated with the outlet.

9. A flow electroporation modular apparatus as claimed in claim 5, wherein: every the connecting pipe groove all has first gyration section, second gyration section and the third gyration section that meets in proper order along self length direction, follows the length direction of base, first gyration section with the third gyration section is located same one side of second gyration section.

10. A flow electroporation modular apparatus as claimed in claim 3, wherein: the axial leads of all the connecting pipe slots are straight lines or smooth arc lines, and the axial leads of all the connecting pipe slots and the axial leads of all the electrode slots extend in a collinear manner.

11. A flow electroporation modular device as claimed in claim 2, wherein: follow the length extending direction of mounting groove, it is protruding to be equipped with a set of or multiunit location in the mounting groove, every group the location is protruding to be located including the branch two of the both sides inner wall of mounting groove, two location protruding with the same cross section of mounting groove is crossing.

12. A flow electroporation modular apparatus as claimed in claim 1, wherein: the inlet and the outlet are respectively arranged at two different sides of the base in the width direction, and the axial lead of the inlet and the axial lead of the outlet are parallel or extend in a collinear way; along the length direction of base, the electricity connection port is located one side of base, the entry with the export is all located the opposite side of base.

13. A flow electroporation modular apparatus as claimed in claim 1, wherein: the electrode assembly comprises two planar electrodes which are arranged at intervals, an electroporation cavity for cell suspension to flow is formed between the two planar electrodes, at least one pair of conductive pieces are arranged in the inner cavity, each pair of conductive pieces comprises a positive conductive piece and a negative conductive piece, the positive conductive piece and the negative conductive piece are respectively electrically connected with the electric connection port, the positive conductive piece and the negative conductive piece are both made of elastic conductive materials, a clamping groove is formed between the positive conductive piece and the negative conductive piece, each group of electrode assemblies is arranged in one clamping groove, and the positive conductive piece and the negative conductive piece are respectively abutted against the planar electrodes on the corresponding side of the electrode assembly.

14. A flow electroporation modular apparatus as claimed in claim 1, wherein: the electroporation component further comprises a liquid inlet pipe and a liquid outlet pipe, the liquid inlet pipe and the liquid outlet pipe are respectively connected with the electrode component, the liquid inlet pipe extends to the outside of the base through the inlet, and the liquid outlet pipe extends to the outside of the base through the outlet.

Images