CN217931391U - Optical fiber system for PCR fluorescence detection - Google Patents

Abstract

The invention relates to the technical field of optical fiber light guide systems, and provides an optical fiber system for PCR (polymerase chain reaction) fluorescence detection, which comprises a main line bundle, a plurality of branch line bundles and an optical fiber line bundle in the process of an optical splitter, wherein an elastic limiting sleeve is sleeved at the joint of the branch line bundles and the optical splitter and fixed on the optical splitter, the elastic limiting sleeve comprises a first sleeve section, a second sleeve section and a third sleeve section which are distributed from the main line bundle to the branch line bundle and have sequentially reduced flexibility, and the flexibility of the third sleeve section is the same as that of the branch line bundles; the elastic limiting sleeve is arranged at the joint of the main wire harness and the branch wire harness, the flexibility of the elastic limiting sleeve is arranged in a segmented mode, the main wire harness gradually softens towards the branch wire harness, a large bending space can be provided for the branch wire harness, the situation that the elastic limiting sleeve is partially excessively bent is avoided, the structure of the elastic limiting sleeve is light, and the branch wire harness can have high degree of freedom.

Description

Optical fiber system for PCR fluorescence detection

Technical Field

The invention relates to the technical field of optical fiber light guide beams, in particular to an optical fiber system for PCR fluorescence detection.

Background

Existing fluorescent quantitative PCR detection devices typically utilize a single light source plus filters and a rotating disk as the light source for multi-wavelength excitation, while achieving multi-channel transmission and detection, with the typical device having multiple optical modules that provide multiple "channels" for optical detection of multiple different dyes, which excite different areas of the rotating disk at any given time and collect the fluorescent energy emitted from the dyes at different wavelengths.

The optical fiber bundle for PCR detection generally comprises a plurality of optical fibers which are placed side by side and combined and encapsulated in a flexible protective sleeve at the end, an optical module is connected to one branch of the optical fiber bundle, the branched optical fibers form a flexible mechanism for receiving fluorescent signals by the optical module without losing sensitivity, but the branched optical fibers need to be flexible and resistant to bending, the existing branched optical fibers are easy to cause signal loss or breakage due to excessive bending in a frequently-active state, the optical fiber bundle additionally provided with a gooseneck tube is heavy in weight, the load of the mechanism is increased, and the flexibility is influenced.

Disclosure of Invention

The invention provides an optical fiber system for PCR fluorescence detection, which comprises an optical fiber harness consisting of a main harness, a plurality of branch harnesses and a light splitter;

a bus bar configured to be connected to the detector;

a plurality of branch wire bundles arranged to be connected with the optical module for collecting fluorescent light energy;

the optical splitter is arranged between the main wiring harness and the branch wiring harness and is used for optically coupling the main wiring harness and the branch wiring harness;

the optical splitter is characterized in that an elastic limiting sleeve is sleeved at the joint of the branch wiring harness and the optical splitter, the elastic limiting sleeve is fixed on the optical splitter, the elastic limiting sleeve comprises a first sleeve section, a second sleeve section and a third sleeve section, the flexibility of the first sleeve section is sequentially reduced from the main wiring harness to the branch wiring harness, and the flexibility of the third sleeve section is the same as that of the branch wiring harness.

Preferably, the elastic limiting sleeve is arranged to be an elastic sleeve made of rubber or silica gel, and the thickness of the elastic sleeve is sequentially reduced by the first sleeve section, the second sleeve section and the third sleeve section.

Preferably, the elastic limiting sleeve is arranged as a spring tube, and the pitch of the spring tube is sequentially increased by the first sleeve section, the second sleeve section and the third sleeve section.

Preferably, the pipe section of the spring tube is rectangular or circular.

Preferably, the length ratio of the first, second and third casing sections is 2:5:3.

preferably, the length of the elastic limiting sleeve is 1/3 to 2/3 of the length of the branch wiring harness.

Preferably, the inner diameter of the elastic limiting sleeve is the same as the outer diameter of the branch wiring harness.

Preferably, the number of the branch harnesses is four or more.

Preferably, the trunk harness or the branch harness comprises an optical fiber core, a filling layer and a sheath layer, the sheath layer is coated on the outer side of the optical fiber core, and the filling layer is filled between the optical fiber core and the sheath layer.

Preferably, the filling layer comprises aramid yarns.

Compared with the prior art, the invention has the advantages that:

according to the invention, the elastic limiting sleeve is arranged at the joint of the main wire harness and the branch wire harness, the flexibility of the elastic limiting sleeve is arranged in sections, and the main wire harness gradually softens towards the branch wire harness, so that a larger bending space can be provided for the branch wire harness, the situation that the elastic limiting sleeve is partially excessively bent is avoided, and the structure of the elastic limiting sleeve is lighter, so that the branch wire harness has higher degree of freedom.

Drawings

The drawings are not intended to be drawn to scale. In the drawings, each identical or nearly identical component that is illustrated in various figures may be represented by a like numeral. For purposes of clarity, not every component may be labeled in every drawing.

FIG. 1 is a schematic structural diagram of an optical fiber system for PCR fluorescence detection according to an embodiment of the present invention.

FIG. 2 is a schematic diagram of a partially enlarged structure of an elastic stop collar of an optical fiber system for PCR fluorescence detection according to an embodiment of the present invention.

Fig. 3 isbase:Sub>A schematic sectional view along the directionbase:Sub>A-base:Sub>A in fig. 2.

FIG. 4 is a schematic diagram of a partially enlarged structure of an elastic stop collar in an optical fiber system for PCR fluorescence detection according to an embodiment of the present invention.

FIG. 5 is a schematic diagram of the internal structure of an optical fiber bundle in the optical fiber system for PCR fluorescence detection according to the embodiment of the present invention.

Detailed Description

In order to better understand the technical content of the present invention, specific embodiments are described below with reference to the accompanying drawings.

The invention aims to provide a light protection mechanism, avoid excessive bending of the branch optical fiber and keep the flexibility of the branch optical fiber, because the existing branch optical fiber is easy to excessively bend at a branch point and the added gooseneck tube can increase the weight.

Referring to fig. 1, the present invention provides an optical fiber system for PCR fluorescence detection, which includes an optical fiber bundle 1 composed of a main bundle 10, a plurality of branch bundles 20, and a splitter 30. A bus harness 10 is provided in connection with the detector.

Wherein, a plurality of branch beams 20 are arranged and connected with the optical module for collecting fluorescence energy, and the optical splitter 30 is arranged between the main beam 10 and the branch beams 20 and is used for optically coupling the main beam 10 and the branch beams 20, thus performing PCR fluorescence detection.

In the embodiment shown in the figure, the elastic limiting sleeve 2 is sleeved at the joint of the branch wiring harness 20 and the optical splitter 30, and the elastic limiting sleeve 2 is fixed on the optical splitter 30.

The elastic limiting sleeve 2 comprises a first sleeve section 21, a second sleeve section 22 and a third sleeve section 23, wherein the flexibility of the first sleeve section 21, the second sleeve section 22 and the third sleeve section 23 is sequentially reduced, the flexibility of the third sleeve section 23 is the same as that of the branch wiring harness 20, and the flexibility of the branch wiring harness 20 is distributed from the main wiring harness 10 to the branch wiring harness 20.

In an alternative embodiment, the ratio of the lengths of the first, second and third casing sections 21, 22, 23 is 2:5:3.

the middle second sheath segment 22 occupies half of the total length, which can provide a larger bending space for the branch harness 20 and protect the branch harness 20 from over bending, thereby providing the branch harness 20 with higher flexibility.

In an alternative embodiment, the elastic spacing sleeve 2 is provided as an elastic sleeve of rubber or silicone.

In particular, in connection with the embodiment shown in fig. 4, the thickness of the elastic sleeve is designed to be reduced by the first sleeve section 21, the second sleeve section 22 and the third sleeve section 23 in sequence, so as to adapt to the purpose that the flexibility of the three sections is reduced in sequence. Wherein the flexibility of the third casing section 23 is the same as the flexibility of the branch wiring harness 20.

Therefore, when the branch harness 20 is bent, the third jacket segment 23 is bent first, and gradually bent toward the second jacket segment 22 and the first jacket segment 21 as the bending proceeds, so that the bending area of the branch harness 20 is lengthened, and the local excessive bending is avoided, thereby protecting the optical fiber core 11 inside, and the elastic jacket of rubber or silicone rubber is light in weight, easy to install, and does not increase the load.

In a further embodiment, shown in connection with fig. 2, the elastic stop collar 2 is provided as a plastic spring tube.

The pitch of the spring tube is sequentially increased by the first sleeve section 21, the second sleeve section 22 and the third sleeve section 23, that is, the pitch of the first sleeve section 21 is greater than the pitch of the second sleeve section 22, and the pitch of the second sleeve section 22 is greater than the pitch of the third sleeve section 23, so that the flexibility of the spring tube is sequentially reduced from the first sleeve section 21 to the second sleeve section 22 and the third sleeve section 23. The configuration makes the flexibility of the third casing section 23 the same as the flexibility of the branch harness 20, so that when the branch harness 20 is bent, the bending radius is gradually increased along with the gradual enhancement of the flexibility of the spring tube, the protection effect is achieved on the branch harness 20, the branch harness 20 is prevented from being excessively bent, the optical fiber core 11 is kept in a low-loss state, and the corresponding plastic spring tube has light weight and can enable the branch harness 20 to have high degree of freedom.

Further, the pipeline section of the spring tube is arranged to be rectangular or circular. Wherein, the spring tube with rectangular cross section has better attaching effect with the branch wire harness 20.

In a further embodiment, the length of the elastic limiting sleeve 2 is 1/3 to 2/3 of the length of the branch wire harness 20, the inner diameter of the elastic limiting sleeve 2 is the same as the outer diameter of the branch wire harness 20, so that the elastic limiting sleeve 2 can limit the bent part of the branch wire harness 20, the damage of the internal optical fiber core 11 caused by the over-bending of the branch wire harness 20 is avoided, and the elastic limiting sleeve 2 is attached to the outer wall of the branch wire harness 20, so that the overall structure of the optical fiber bundle is more compact.

In an alternative embodiment, the number of branch beams 20 may be adapted to the detection device, and the branch beams are configured according to the sample set, and 4 are illustrated in fig. 1 as an example. In the example shown in fig. 3, the branched beams 20 are seven, so that seven groups of samples can be simultaneously detected during PCR fluorescence detection.

As shown in fig. 5, each of the optical fiber bundles of the main bundle 10 or the branch bundles 20 includes an optical fiber core 11, a filler layer 12, and a sheath layer 13.

The inner core of the optical fiber core 11 is made of low-loss high-purity quartz glass fiber, and the outer side of the optical fiber core is coated with a cladding made of silica doped with materials, so that the refractive index of the cladding is far lower than that of the fiber core, the lowest loss of the fiber core is about 0.47db/km, and the optical fiber core has high transmission rate.

The sheath layer 13 is coated on the outer side of the optical fiber core 11 and is formed by extruding polyethylene plastics.

The filling layer 12 is filled between the optical fiber core 11 and the sheath layer 13. In a preferred embodiment, the filling layer 12 is made of aramid yarn, and the aramid yarn has special properties such as low density, very high tensile modulus, high breaking strength, low breaking elongation, and the like, and has a good protection effect on the optical fiber core 11.

With the above embodiments, in the design of the optical fiber system for PCR detection provided by the present invention, the elastic stop collar 2 is disposed at the connection position of the main harness 10 and the branch harness 20, the flexibility of the elastic stop collar 2 is set in sections, and the main harness 10 gradually softens towards the branch harness 20, so as to provide a larger bending space for the branch harness 20, avoid the situation that the elastic stop collar 2 is partially over-bent, and the structure of the elastic stop collar 2 is lighter, so that the branch harness 20 has a higher degree of freedom.

Although the present invention has been described with reference to the preferred embodiments, it is not intended to be limited thereto. Those skilled in the art can make various changes and modifications without departing from the spirit and scope of the invention. Therefore, the protection scope of the present invention should be defined by the appended claims.

Claims (9)

1. An optical fiber system for PCR fluorescence detection, comprising an optical fiber bundle (1) composed of a main bundle (10), a plurality of branch bundles (20) and a splitter (30), wherein:

a bus bar (10) arranged in connection with the detector;

a plurality of branch strands (20) disposed in connection with the optical module for collecting fluorescent light energy;

a beam splitter (30) disposed between the main beam (10) and the branch beam (20) and optically coupling the main beam (10) and the branch beam (20);

wherein, elasticity stop collar (2) are established to the junction cover of branch pencil (20) and optical splitter (30), elasticity stop collar (2) are fixed on optical splitter (30), elasticity stop collar (2) include by total pencil (10) to first cover section (21), second cover section (22) and third cover section (23) that the flexibility that branch pencil (20) direction distributed reduces in proper order, the flexibility of third cover section (23) with the flexibility of branch pencil (20) is the same.

2. The optical fiber system for PCR fluorescence detection according to claim 1, wherein the elastic stop collar (2) is configured as an elastic collar of rubber or silicone, and the thickness of the elastic collar is sequentially reduced by the first sleeve section (21), the second sleeve section (22) and the third sleeve section (23).

3. The optical fiber system for PCR fluorescence detection according to claim 1, wherein the elastic stop collar (2) is configured as a spring tube having a pitch that is sequentially increased by a first sleeve section (21), a second sleeve section (22) and a third sleeve section (23).

4. The optical fiber system for PCR fluorescence detection according to claim 3, wherein the tube section of the bourdon tube is rectangular or circular.

5. The optical fiber system for PCR fluorescence detection according to claim 1, wherein the length ratio of the first, second and third casing sections (21, 22, 23) is 2:5:3.

6. the optical fiber system for PCR fluorescence detection according to claim 1, wherein the length of the elastic stop collar (2) is 1/3 to 2/3 of the length of the branch harness (20).

7. The optical fiber system for PCR fluorescence detection according to claim 1, wherein the length of the second sheath segment (22) is half of the length of the elastic stop collar (2).

8. The optical fiber system for PCR fluorescence detection according to claim 1, wherein an inner diameter of the elastic stopper sleeve (2) is the same as an outer diameter of the branch harness (20).

9. The optical fiber system for PCR fluorescence detection according to claim 1, wherein the trunk harness (10) or the branch harness (20) each comprises an optical fiber core (11), a filling layer (12) and a sheath layer (13), the sheath layer (13) is coated on the outer side of the optical fiber core (11), and the filling layer (12) is filled between the optical fiber core (11) and the sheath layer (13).

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