CN217931369U - Light path system and microplate reader - Google Patents

Abstract

The application discloses optical path system and ELIASA, optical path system includes: the light source assembly comprises a convex lens and an LED lamp source arranged on one side of the convex lens; the light splitting assembly comprises a light splitter and a plurality of optical fibers, the input end of the light splitter is arranged at a light spot position where the diameter is within a first preset threshold range, a plurality of light splitting holes are formed in the light splitter, the input end of each light splitting hole is used for receiving the light spot, the input end of each optical fiber is connected with the output end of each light splitting hole, the output end of each optical fiber comprises a reference hole and a sample hole, and each sample hole is connected with a microplate hole. The microplate reader comprises the light path system, a controller and a microplate, wherein the controller is connected with the light splitting assembly and the microplate and is used for calibrating the light intensity of the sample hole according to the light intensity change of the reference hole. The light source spectrum is stable, and the inter-station difference debugging is also simpler.

Description

Light path system and microplate reader

Technical Field

The application relates to the technical field of medical inspection, in particular to a light path system and an enzyme-labeling instrument.

Background

The enzyme-linked immunosorbent assay (ELISA) instrument is a professional instrument for reading and analyzing ELISA test results, and the core of the ELISA instrument is a colorimeter, namely, the colorimetry is used for analyzing the content of antigens or antibodies. The enzyme-linked immunosorbent assay is carried out by enzyme-catalyzed chromogenic substrates coupled on the antigen or the antibody, the reaction result is displayed in color, and the concentration of the antibody or the antigen to be detected in the sample can be judged by the color depth, namely the magnitude of the absorbance value. In enzyme-linked immunosorbent assays, the accuracy of temperature control directly affects the accuracy of enzyme activity and immunoassay result determination.

Currently, microplate readers generally employ halogen lamps as light sources. The spectrum of the halogen lamp is 400nm-800nm, and the microplate reader only needs to select 4 commonly used spectra, such as 405nm, 450nm, 492nm and 630nm, and adopts a method of switching filter lenses for obtaining. However, the halogen lamp is adopted as the light source, and has the following defects: the heating value is large, the light attenuation is serious, and the serious light attenuation can be shown due to heat after the lamp is continuously lightened for a few minutes, so that the normal work is influenced; the debugging difficulty of the inter-platform difference of the equipment is high, and even if the same standard substance is tested by the same batch of machines, the equipment can show larger inter-platform difference; the service life is short, and the halogen lamp is used as a light source, and the bulb needs to be replaced in about one year generally.

SUMMERY OF THE UTILITY MODEL

For solving the problem that traditional ELIASA light source life-span is short, calorific capacity is big, light decay is serious and the poor debugging degree of difficulty is big between the platform, this application provides a light path system and ELIASA, and not only the poor debugging between the equipment platform is simple, can effectively reduce the light source calorific capacity moreover, reduce light decay, can also increase of service life.

An optical path system according to an embodiment of the first aspect of the present application includes: the light source assembly comprises a convex lens and an LED lamp source arranged on one side of the convex lens; the LED light source forms light spots with different diameters at the positions of the other side of the convex lens and the convex lens at different distances; the optical splitter comprises an optical splitter and a plurality of optical fibers, wherein the input end of the optical splitter is arranged at a spot with the diameter within a first preset threshold range, a plurality of light splitting holes are formed in the optical splitter, the input ends of the light splitting holes are used for receiving the light spots, the input ends of the optical fibers are connected with the output ends of the light splitting holes, the output ends of the optical fibers comprise reference holes and sample holes, and the sample holes are connected with the microplate holes; the micro-plate hole is internally loaded with an experimental sample, a plurality of light splitting holes connected with the sample holes are evenly distributed on a concentric circle, the light splitting holes connected with the reference holes are arranged at the circle center of the concentric circle, and the first preset threshold range is arranged according to the diameter of the cross section of the light splitter.

According to the optical path system of the embodiment of the first aspect of the present application, at least the following beneficial effects are provided: the LED lamp is used as a light source, so that the problems of large heating value, serious light attenuation and short service life when the traditional halogen lamp is used as the light source can be effectively avoided, the spectrum of the LED light source is relatively stable, and the debugging of the inter-station difference is simple; set up first preset threshold value scope according to optical splitter cross-section diameter, and set up the input of optical splitter in the facula department that forms the diameter and be first preset threshold value scope, make the input phase-match that forms facula size and optical splitter, again with a plurality of light splitting holes average distribution who is connected with a plurality of sample holes on a concentric circle, make the received light intensity of each light splitting hole more unanimous, and then the light intensity of exporting a plurality of sample holes is also unanimous, thus, make the light that a plurality of microplate holes received unanimous, can guarantee the uniformity of a plurality of microplate hole experimental results.

According to some embodiments of the present application, the optical path system further comprises a linkage mechanism, the linkage mechanism is provided with a plurality of LED light sources of different wave bands, and the linkage mechanism is used for switching the LED light sources. According to the experiment requirements of different users, when enzyme-linked immunosorbent assay is carried out, light rays with various wave bands are used, for example, the spectrum range of halogen lamps is 400nm-800nm, and several spectra are usually selected for carrying out the experiment. The spectral range of the LED lamp is narrow, the requirement of a user on a wide spectral range can not be met by emitting light by one LED lamp source, the LED lamp sources with a plurality of different wave bands are arranged on the linkage mechanism, and an operator can switch different LED lamp sources according to needs so as to meet the experiment requirements of different users.

Furthermore, the linkage mechanism is also provided with a plurality of optical filters, the optical filters are arranged between the convex lens and the optical splitter, and the linkage mechanism is used for simultaneously switching the LED light sources and the optical filters with corresponding wave bands; wherein, each optical filter corresponds to the LED lamp source one by one. In order to avoid interference of natural light on the light received by the input end of the light splitting hole, the light splitter and the convex lens are directly provided with the light filters corresponding to different LED light sources, so that the natural light mixed in the light path system can be effectively filtered, and the accuracy of the experiment is further ensured.

Further, the linkage mechanism comprises a first driving unit, a first conveying belt, a connecting portion, a lamp source base and an optical filter base, one end of the connecting portion is fixedly connected with the lamp source base, the other end of the connecting portion is connected with the optical filter base, the middle of the connecting portion is fixedly connected with the first conveying belt, a plurality of LED lamp sources are arranged on the lamp source base, and a plurality of optical filters are arranged on the optical filter base. The LED light sources are arranged on the light source base, the optical filters are arranged on the optical filter base, the light source base and the optical filter base are connected through the connecting portions, the connecting portions are fixed on the first conveying belt, the first conveying belt is driven by the first driving unit to move, the connecting portions are driven by the first conveying belt, the LED light sources and the optical filters are driven to move simultaneously, linkage switching of the LED light sources and the optical filters is finally achieved, and the purpose of obtaining light with target wavelength is achieved.

Further, the link gear still includes the slide rail, and the slide rail sets up in the connecting portion below, connecting portion and slide rail sliding connection. Through set up the slide rail in connecting portion below, can play the effect of a support to connecting portion on the one hand, reduce the atress of first conveyer belt in vertical direction, make connecting portion along with first conveyer belt along the motion of slide rail direction, finally realize LED lamp source base and light filter base and switching LED lamp source and the more smooth and easy purpose of light filter.

According to some embodiments of the present application, the sample wells are provided with 4 or 8, the spectroscopic wells connected to the 4 or 8 sample wells are evenly distributed on a concentric circle, and the spectroscopic wells connected to the reference wells are provided at the center of the concentric circle. By arranging 4 or 8 light splitting holes which are evenly distributed on a concentric circle and a reference hole which is arranged at the center of the concentric circle, when the position of the LED lamp source is calibrated, the LED lamp source can be quickly and accurately adjusted in place by taking the light splitting holes which are evenly distributed on the concentric circle and the center of the concentric circle as reference.

According to some embodiments of the present application, the output end of the optical splitter is provided with a fixing head for fixing a connection end of the optical fiber and the output end of the optical splitter. In order to avoid the problem that the light output to a sample hole or a reference hole is weak or unstable due to unstable falling or poor contact of the output end of the optical splitter and the optical fiber connection part, the output end of the optical splitter is provided with a fixing head, the output end of the optical splitter and the optical fiber connection part are fixed by one section, and the stability of the output light can be improved.

According to some embodiments of the present application, the light source assembly further includes a first light channel disposed between the LED light source and the light splitter, and the convex lens is disposed inside the first light channel. In order to further reduce the natural light irradiated on the optical filter and the natural light entering the input end of the optical splitter, and avoid the problem of inaccurate experimental results, a first optical channel is arranged between the LED lamp source and the optical filter, and the optical filter is arranged inside the first optical channel, so that the interference of the natural light can be effectively eliminated, and the purity of the light of the whole light path system is ensured.

In a second aspect of the present application, a microplate reader is provided, which comprises the optical path system provided in the first aspect of the present application.

According to some embodiments of the application, the microplate reader further comprises a controller, the controller is connected with the light splitting assembly, and the controller is used for calibrating the light intensity of the sample hole according to the light intensity change of the reference hole. Through setting up the reference hole, can in time compensate the calibration to the light intensity in sample hole according to the light intensity change in reference hole to guarantee the accuracy of experimental result.

Additional aspects and advantages of the present application will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the present application.

Drawings

For a clearer explanation of the embodiments of the present application or technical solutions in the prior art, the drawings needed to be used in the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments described in the present application, and other drawings can be obtained by those skilled in the art according to the drawings.

Fig. 1 is a schematic overall structure diagram of an optical path system according to an embodiment of the present application;

FIG. 1a is a partially enlarged view of an output end of a beam splitter of an optical path system according to an embodiment of the present application;

fig. 2 is a schematic structural diagram of a linkage mechanism of an optical path system according to an embodiment of the present application;

FIG. 3 is a schematic structural diagram of another angle of the linkage mechanism of the optical path system according to the embodiment of the present application;

fig. 4 is a schematic structural diagram of a beam splitter of an optical path system according to an embodiment of the present application;

fig. 5 is a schematic view of another angle structure of the beam splitter of the optical path system according to the embodiment of the present application.

Reference numerals:

the light source assembly 100, the LED lamp source 110, the convex lens 120, the first light channel 130, the optical filter 140, the light splitting assembly 200, the light splitting hole 210, the fixing head 220, the linkage mechanism 300, the first driving unit 310, the first conveyor belt 320, the lamp source base 330, the optical filter base 340, the connecting portion 350, the slide rail 360, the optical fiber output end 400, the sample hole 410, and the reference hole 420.

Detailed Description

Reference will now be made in detail to the embodiments of the present application, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present application and are not to be construed as limiting the present application.

In the description of the present application, it is to be understood that the positional descriptions, such as the upper, lower, left, right, front, rear, and the like, referred to as positional or orientational relationships based on the drawings are only for convenience of description and simplicity of description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present application.

In the description of the present application, the meaning of plural is two or more, and greater than, less than, etc. are understood as excluding the present number, and not greater than, less than, inner, etc. are understood as including the present number. The description of first, second, etc. in this application is for the purpose of distinguishing between technical features and is not intended to indicate or imply relative importance or to implicitly indicate the number of technical features indicated or to implicitly indicate the precedence of technical features indicated.

In the description of the present application, unless otherwise expressly limited, terms such as set, connected and the like should be construed broadly, and those skilled in the art can reasonably determine the specific meanings of the above terms in the present application in consideration of the detailed contents of the technical solutions.

As shown in fig. 1, in a first aspect of the present application, an optical path system is provided, including: the light source assembly 100, the light source assembly 100 includes a convex lens 120, and an LED light source 110 disposed on one side of the convex lens 120; the LED light source 110 forms light spots with different diameters on the other side of the convex lens 120 and at different distances from the convex lens 120; the optical splitting assembly 200 comprises an optical splitter and a plurality of optical fibers, wherein the input end of the optical splitter is arranged at a spot with a diameter within a first preset threshold range, a plurality of light splitting holes 210 are formed in the optical splitter, the input end of each light splitting hole 210 is used for receiving the spot, the input end of each optical fiber is connected with the output end of the corresponding light splitting hole 210, the output end 400 of each optical fiber comprises a reference hole 420 and a plurality of sample holes 410, and the sample holes 410 are connected with the microplate holes; the microplate is loaded with an experimental sample in the hole, the plurality of light splitting holes 210 connected with the plurality of sample holes 410 are evenly distributed on a concentric circle, the light splitting holes 210 connected with the reference holes 420 are arranged at the center of the concentric circle, and the first preset threshold range is arranged according to the diameter of the cross section of the light splitter.

Specifically, the LED light source 110 is disposed on one side of the convex lens 120, and the beam splitter is disposed on the other side of the convex lens 120. Light emitted by the LED light source 110 is focused by the convex lens 120, and then forms a light spot on the other side of the convex lens 120, wherein the diameter of the light spot changes along with the change of the distance between the light spot and the convex lens 120, the input end of the light splitter is arranged on the other side of the convex lens 120, and the light spot with the diameter being within a first preset threshold range is formed, and the first preset threshold range is arranged according to the diameter of the cross section of the light splitter, namely, the light splitter is arranged at the position where the diameter of the light spot is equivalent to the diameter of the cross section of the light splitter. The diameters of the two are equal in size, namely, the difference value of the diameters of the two is within a preset range, so that if the light splitter is determined, the value of the section diameter of the light splitter can be obtained, according to the preset range of the difference value of the diameters of the two, the diameter of the light spot can be determined to be a first preset threshold range, and finally the position range of the light splitter placed on the other side of the convex lens 120 can be determined.

The spectroscopic wells 210 connected to the sample wells 410 of the microplate are evenly distributed on a concentric circle, and the reference wells 420 are distributed at the center of the concentric circle. The light connects the microplate holes and the light splitting holes 210, that is, each microplate hole uniquely corresponds to one light splitting hole 210. The light spots irradiate on the concentric circle, the light intensities received by the light splitting holes 210 at the same radius away from the center of the concentric circle are the same, namely, the light intensities received by the light splitting holes 210 evenly distributed on the concentric circle and transmitted to the sample holes 410 are the same, namely, the light energy transmitted in each optical fiber at the output end of the light splitter can be ensured to be consistent as much as possible, and the accuracy of simultaneous experiments of a plurality of samples is ensured. The light splitting hole 210 positioned at the center of the concentric circle is connected with the reference hole 420, so that the change of the light intensity can be monitored in real time, the controller can calibrate the light intensity of the sample hole 410 in time according to the change of the light intensity of the reference hole 420, and the accuracy of the experimental result is further improved.

Referring to the enlarged view a of the portion of the spectrometer in fig. 1a, 9 located at the center of the concentric circle is connected to the reference well 420 on the microplate, and a plurality of spectroscopic wells 210 connected to the remaining plurality of sample wells 410 are evenly distributed on the concentric circle.

According to the scheme, the LED lamp is used as the light source, so that compared with the traditional halogen lamp which is used as the light source, the heating amount can be effectively reduced, the light attenuation is reduced, and the service life is prolonged; the first preset threshold range of the light spot diameter received by the input end of the optical splitter is set according to the cross section diameter of the optical splitter, the input end of the optical splitter is arranged at the position of the light spot with the diameter being the first preset threshold range, the size of the formed light spot is matched with the input end of the optical splitter, a plurality of light splitting holes 210 connected with a plurality of sample holes 410 are evenly distributed on a concentric circle, the light intensity received by each light splitting hole 210 is consistent, the light intensity output to the sample holes 410 is consistent, and the consistency of the experiment results of the microplate holes can be ensured. Compared with a halogen lamp, the LED lamp has the advantages that the heat productivity is large, the light attenuation is serious, the fluctuation is serious, the problem that the compensation difficulty is large and the debugging difficulty of the inter-platform difference is large is caused, the light attenuation is small when the LED lamp is used as a light source, the fluctuation is small, the spectrum is relatively stable, and the inter-platform difference debugging is simple.

Further, the sample wells 410 connected to the spectroscopic wells 210 are provided in 4 or 8 numbers, the spectroscopic wells 210 are evenly distributed on a concentric circle, and the spectroscopic wells 210 connected to the reference wells 420 are provided at the center of the concentric circle. The center of the circle is used as a central point, and the other 4 or 8 light splitting holes 210 are used as reference points in different directions, so that the position of the LED lamp source 110 can be correspondingly adjusted, light spots formed by light rays emitted by the LED lamp source 110 after being focused by the convex lens 120 just fall on the input end of the light splitter, and thus, the positions of the LED lamp source 110 can be adjusted by the light splitting holes 210 in a more convenient and faster calibration manner when the light splitting holes 210 receive the light rays, and the experimental efficiency is improved.

Referring to fig. 2 and 3, in some embodiments of the present application, the optical path system further includes a linkage 300, the linkage 300 is provided with a plurality of LED light sources 110 with different wavelength bands, and the linkage 300 is used for switching the LED light sources 110. In enzyme-linked immunosorbent assays, various bands of light are used, for example, a halogen lamp having a spectral range of 400nm to 800nm, several of which are usually selected for the assay. The spectral range of the LED lamp is narrow, the requirement of a user on a wide spectral range cannot be met when one LED lamp source 110 emits light, the linkage mechanism 300 is provided with a plurality of LED lamp sources 110 with different wave bands, and an operator can switch different LED lamp sources 110 as required, so that the experiment requirements of different users are met.

Specifically, 4 LED lamps having center wavelengths of 405nm, 450nm, 492nm, and 630nm, respectively, may be provided as the lamp sources. If the four LED light sources 110 are needed for the enzyme-linked immunosorbent assay, and the 96-well microplate is divided into 12 rows and 8 columns, after one LED light source 110 irradiates, the experimental results of the samples in each row of microplate wells when the light source irradiates are sequentially read for 12 times, then the next LED light source 110 is switched, and so on, the samples are sequentially read for 12 times under each LED light source 110 until all the LED light sources 110 finish irradiating, and then the linkage mechanism 300 drives the LEDs to return to the original positions. Therefore, the switching times of the LED lamp sources 110 can be reduced, all samples on the same microplate are irradiated by the same light source, and the consistency of experimental results is ensured.

Of course, one or more LED lamps with center wavelengths may be selected as the light source, or LED lamps with other center wavelengths may be selected as the light source, and the number and the center wavelengths of the LED light sources 110 are merely exemplary and are not limited to the present disclosure.

Further, the linkage mechanism 300 is further provided with a plurality of optical filters 140, the optical filters 140 are arranged between the convex lens 120 and the optical splitter, and the linkage mechanism 300 is used for simultaneously switching the LED light sources 110 and the optical filters 140 of corresponding wave bands; each of the optical filters 140 corresponds to the LED light source 110. In order to avoid interference of natural light on the light received by the input end of the light splitting hole 210, the light splitter and the convex lens 120 are directly provided with the light filter 140 corresponding to different LED light sources 110, so that the natural light mixed in the light path system can be effectively filtered, and the accuracy of the experiment is further ensured.

Correspondingly, in order to avoid ambient light entering the beam splitter, a filter 140 is disposed between the beam splitter and the convex lens 120. Specifically, 4 narrowband filters 140 corresponding to the 4 LED light sources 110 with central wavelengths of 405nm, 450nm, 492nm, and 630nm are provided, so that interference of ambient light in the optical path system can be effectively eliminated, and accuracy of the experiment can be improved.

Further, the linkage mechanism 300 includes a first driving unit 310, a first conveyor belt 320, a connecting portion 350, a lamp source base 330 and a filter base 340, wherein one end of the connecting portion 350 is fixedly connected with the lamp source base 330, the other end of the connecting portion is connected with the filter base 340, the middle of the connecting portion 350 is fixedly connected with the first conveyor belt 320, the lamp source base 330 is provided with a plurality of LED lamps 110, and the filter base 340 is provided with a plurality of filters 140. The LED lamp sources 110 are arranged on the lamp source base 330, the optical filters 140 are arranged on the optical filter base 340, the lamp source base 330 and the optical filter base 340 are connected through the connecting portion 350, the connecting portion 350 is fixed on the first conveyor belt 320, the first conveyor belt 320 is driven to move by the first driving unit 310, the first conveyor belt 320 drives the connecting portion 350, the LED lamp sources 110 and the optical filters 140 are driven to move simultaneously, and finally linkage switching of the LED lamp sources 110 and the optical filters 140 is achieved, and light rays with target wavelengths are obtained.

Further, the linkage mechanism 300 further includes a slide rail 360, the slide rail 360 is disposed below the connecting portion 350, and the connecting portion 350 is slidably connected to the slide rail 360. By arranging the slide rail 360 below the connecting portion 350, on one hand, the connecting portion 350 can be supported, the stress of the first conveyor belt 320 in the vertical direction is reduced, the connecting portion 350 moves along the slide rail 360 along with the first conveyor belt 320, and finally, the purpose that the lamp source base 330 and the optical filter base 340 are smoother in switching the LED lamp source 110 and the optical filter 140 is achieved.

Specifically, the first driving unit 310 may be configured as a motor, the motor drives the first conveyor belt 320 to move, and the first conveyor belt 320 drives the connecting portion 350 connected thereto to move horizontally, so as to drive the lamp source base 330 and the optical filter base 340 to move synchronously.

It is understood that fig. 2 and 3 show an example in which the lamp base 330 and the filter base 340 are horizontally disposed, and the LED lamp 110 and the filter 140 are synchronously switched by horizontally moving the lamp base 330 and the filter base 340; the lamp source base 330 and the optical filter base 340 can be vertically arranged, the LED lamp source 110 and the optical filter 140 can be synchronously switched by moving the lamp source base 330 and the optical filter base 340 up and down, and the first driving unit 310 can be a cylinder corresponding to the lamp source base 330 and the optical filter base 340, and the cylinder drives the connecting part 350 to move up and down, so that the lamp source base 330 and the optical filter base 340 are driven to move up and down, and the purpose of switching the LED lamp source 110 and the optical filter 140 in a linkage manner is achieved.

In some embodiments of the present application, the light source assembly 100 further includes a first light channel 130, the first light channel 130 is disposed between the LED light source 110 and the light splitter, and the convex lens 120 is disposed inside the first light channel 130. When the whole optical path system is not closed enough, in order to further reduce the natural light irradiated to the optical filter 140 and the natural light entering the input end of the optical splitter, and avoid the problem of inaccurate experimental result, a first optical channel 130 is arranged between the LED light source 110 and the optical filter 140, and the optical filter 140 is arranged inside the first optical channel 130, so that the interference of the natural light can be effectively eliminated, and the purity of the light of the whole optical path system is ensured.

Referring to fig. 4 and 5, in some embodiments of the present application, the output end of the optical splitter is provided with a fixing head 220, and the fixing head 220 is used for fixing the connection end of the optical fiber and the output end of the optical splitter. In order to avoid the problem that the connection between the output end of the optical splitter and the optical fiber is unstable and falls off or is not good enough to finally cause weak or unstable light output to the sample hole 410 or the reference hole 420, the output end of the optical splitter is provided with the fixing head 220, the connection between the output end of the optical splitter and the optical fiber is fixed by one section, and the stability of the output light can be improved.

Specifically, the fixing head 220 may be a hollow cylinder, and the inner diameter of the fixing head may be set according to the diameter of the aforementioned concentric circle, so as to ensure that the optical fiber connecting the output end of the optical splitter and the hole of the micro plate may pass through the middle of the fixing head 220. Of course, the filler can be arranged between the optical fibers in the fixing head 220, so that the positions of the optical fibers in the fixing head 220 can be further fixed, and the optical fibers are prevented from shaking in the fixing head 220 to influence the stability of connection between the optical fibers and the output end of the optical splitter.

In a second aspect of the present application, a microplate reader is provided, which comprises the optical path system provided in the first aspect of the present application.

In some embodiments of the present application, the microplate reader further comprises a controller coupled to the light splitting assembly 200 for calibrating the light intensity of the sample wells 410 to the light intensity variations of the reference wells 420. By arranging the reference hole 420, the light intensity of the sample hole 410 can be compensated and calibrated in time according to the light intensity change of the reference hole 420, so as to ensure the accuracy of the experimental result.

Further, the controller is further connected with the linkage mechanism 300, and the controller can set the LED light sources 110 which are sequentially switched to the preset wave band according to the experiment requirement, so as to improve the experiment efficiency.

When the optical fiber micro-plate is used, the optical fiber output end 400 is aligned to a row of micro-plate holes, the LED light source 110 with the central wavelength of 405nm and the corresponding optical filter 140 are used, the optical filter 140 with the central wavelength of 405nm is also used, if the micro-plate is provided with 8X12 holes, the position of the micro-plate is sequentially switched for 12 times by the optical fiber output end 400, and the controller respectively reads and records the absorbance of each sample under the LED light source 110; and then switching the next LED light source 110 with the central wavelength of 450nm, wherein the linkage mechanism 300 is in linkage control to automatically switch the optical filter 140 with the corresponding central wavelength, and then sequentially switching the positions of the micro-plates for 12 times under the light source, and the controller respectively reads and records the absorbance of each sample under the LED light source 110, and so on. And finally, calculating the absorbance of each sample under the LED light source with each central wavelength by the controller to obtain the experimental result of the enzyme-linked immunosorbent assay of all samples.

Of course, in order to increase the flexibility of the experiment, the linkage mechanism 300 may also be manually controlled to switch the LED light source 110 in the preset waveband.

It should be noted that, for the sake of simplicity, the above-mentioned method embodiments are described as a series of acts or combinations, but those skilled in the art should understand that the present application is not limited by the described order of acts, as some steps may be performed in other orders or simultaneously according to the present application. Further, those skilled in the art should also appreciate that the embodiments described in the specification are preferred embodiments and that the acts and modules referred to are not necessarily required in this application.

In the description herein, reference to the description of the terms "one embodiment," "some embodiments," "an illustrative embodiment," "an example," "a specific example," or "some examples" or the like means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the application. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The applicant asserts that the above-described embodiments merely represent the basic principles, principal features and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are merely illustrative of the principles of the invention, and that various changes and modifications can be made by one skilled in the art without departing from the spirit and scope of the invention, which will fall within the scope of the invention as claimed.

Although embodiments of the present application have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the application, the scope of which is defined in the claims and their equivalents.

Claims (10)

1. An optical path system, comprising:

the LED lamp comprises a light source assembly and a light source, wherein the light source assembly comprises a convex lens and an LED lamp source arranged on one side of the convex lens; the LED light source forms light spots with different diameters at the positions, at different distances from the convex lens, on the other side of the convex lens;

the spectrometer comprises a light splitting assembly and a plurality of optical fibers, wherein the input end of the light splitting assembly is arranged at a spot with a diameter within a first preset threshold range, the light splitting assembly is provided with a plurality of light splitting holes, the input ends of the light splitting holes are used for receiving the light spots, the input ends of the optical fibers are connected with the output ends of the light splitting holes, the output ends of the optical fibers comprise reference holes and sample holes, and the sample holes are connected with microplate holes; the microplate is characterized in that an experimental sample is loaded in the microplate hole, a plurality of light splitting holes connected with the sample holes are evenly distributed on a concentric circle, the light splitting holes connected with the reference holes are arranged at the center of the concentric circle, and the first preset threshold range is arranged according to the diameter of the cross section of the light splitter.

2. The optical path system according to claim 1, further comprising a linkage mechanism, wherein the linkage mechanism is provided with a plurality of LED light sources with different wavelength bands, and the linkage mechanism is configured to switch the LED light sources.

3. The optical path system according to claim 2, wherein a plurality of optical filters are further disposed on the linkage mechanism, the optical filters are disposed between the convex lens and the optical splitter, and the linkage mechanism is configured to simultaneously switch the LED light sources and the optical filters in corresponding wavelength bands; and each optical filter corresponds to the LED light source one to one.

4. The optical system according to claim 3, wherein the linkage mechanism includes a first driving unit, a first transmission belt, a connecting portion, a lamp source base, and a filter base, one end of the connecting portion is fixedly connected to the lamp source base, the other end of the connecting portion is connected to the filter base, a middle portion of the connecting portion is fixedly connected to the first transmission belt, the lamp source base is provided with a plurality of the LED lamps, and the filter base is provided with a plurality of the filters.

5. The optical system according to claim 4, wherein the linkage mechanism further comprises a slide rail disposed below the connecting portion, and the connecting portion is slidably connected to the slide rail.

6. The optical system according to claim 1, wherein the sample well is provided with 4 or 8 sample wells, the spectroscopic wells connected to the 4 or 8 sample wells are evenly distributed on a concentric circle, and the spectroscopic well connected to the reference well is provided at a center of the concentric circle.

7. The optical system according to claim 1, wherein the output end of the optical splitter is provided with a fixing head, and the fixing head is used for fixing the connecting end of the optical fiber and the output end of the optical splitter.

8. The optical system according to any one of claims 1 to 7, wherein the light source assembly further includes a first light channel disposed between the LED light source and the beam splitter, and the convex lens is disposed inside the first light channel.

9. A microplate reader, characterized in that it comprises an optical path system according to any one of claims 1 to 8.

10. The microplate reader of claim 9, further comprising a controller connected to the light splitting assembly, the controller configured to calibrate the light intensity of the sample well based on changes in the light intensity of the reference well.

Images