CN217922047U

CN217922047U - Novel adipose-derived stem cell exosome culture device

Info

Publication number: CN217922047U
Application number: CN202221534254.8U
Authority: CN
Inventors: 郭巧; 牟浩; 李伟
Original assignee: TIANJIN WEIKAI BIOENG Ltd
Current assignee: TIANJIN WEIKAI BIOENG Ltd
Priority date: 2022-06-16
Filing date: 2022-06-16
Publication date: 2022-11-29
Anticipated expiration: 2032-06-16

Abstract

The utility model relates to a cell culture technical field, specific novel adipose-derived stem cell exosome culture device that says so, including the device main part, the inside of device main part is equipped with multilayer baffle, and multilayer baffle is with a plurality of cultivation storehouses of the inside partition department of device main part, and every there is the pull box in the equal slidable mounting in inside of cultivation storehouse, the upper surface of pull box is equipped with multiunit fixture, fixture includes movable rod, dead lever, draws piece and rotary disk, the inside of pull box is the cavity setting, and the upper surface corresponds dead lever, movable rod position department level and has seted up the spout, spout fixed connection is passed to the bottom of dead lever, the movable rod bottom pass the spout and with spout sliding connection, the bottom lateral wall fixedly connected with arm-tie of movable rod, the arm-tie level sets up and keeps away from the first spring of one end fixedly connected with of movable rod. This novel adipose-derived stem cell exosome culture device to the fixed mode simple operation of cultivateing the household utensils.

Description

Novel adipose-derived stem cell exosome culture device

Technical Field

The utility model belongs to the technical field of cell culture, especially, relate to a novel adipose-derived stem cell exosome culture device.

Background

Adipose-derived stem cell is the stem cell that obtains from adipose tissue separation in recent years has multidirectional differentiation potential, mainly resume histocyte's repairing function, in order to promote the quality of the cultivation of cell, can use culture apparatus to carry out the assisted cultivation in cultivating the in-process, application number CN213113363U discloses a novel adipose-derived stem cell exosome culture apparatus among the prior art, stir two clamp splices and fix culture apparatus, need both hands to stir, place and loosen the clamp splice again behind the household utensils, one-man operation is difficult to realize, it is not convenient to use to the fixed mode of household utensils.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving prior art, application number CN213113363U discloses a novel adipose-derived stem cell exosome culture device, stirs two clamp splices and fixes culture device, needs both hands to stir, loosens the clamp splice after placing the household utensils again, and one-man operation is difficult to realize, uses inconvenient problem to the fixed mode of household utensils, and the novel adipose-derived stem cell exosome culture device who provides.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a novel adipose-derived stem cell exosome culture device comprises a device main body, wherein multiple layers of partition plates are arranged inside the device main body, the multiple layers of partition plates divide the inside of the device main body into multiple culture bins, a drawing box is slidably mounted inside each culture bin, and multiple groups of clamping mechanisms are arranged on the upper surface of each drawing box;

the clamping mechanism comprises a movable rod, a fixed rod, a pull block and a rotating disc, the interior of the pull box is hollow, a sliding groove is horizontally formed in the position, corresponding to the position of the fixed rod and the position of the movable rod, of the upper surface of the pull box, the bottom of the fixed rod penetrates through the sliding groove to be fixedly connected, the bottom of the movable rod penetrates through the sliding groove to be slidably connected with the sliding groove, a pull plate is fixedly connected to the side wall of the bottom end of the movable rod, the pull plate is horizontally arranged, one end, away from the sliding rod, of the pull plate is fixedly connected with a first spring, a driving gear meshed with the pull plate is arranged on the side of the pull plate, a tooth socket meshed with the driving gear is formed in the pull plate, one side, away from the pull plate, of the driving gear is clamped with a follow-up block, and the upper surface of the driving gear is fixedly connected with the rotating disc;

the follow-up block is hinged with the lifting block, the lifting block is elastically arranged, the bottom of the lifting block is provided with a bottom block, a pulling block is arranged above the lifting block, the pulling block is of a hollow rectangular structure and extends out of the upper surface of the drawing box.

Benefit from that the cooperation of pull box and fixture can be stable fixed with the culture ware, promote the stability and the independence that the culture ware was placed.

Preferably, one end of the first spring, which is far away from the pulling plate, is fixedly connected with a fixing block, and the fixing block is fixedly installed inside the pulling box.

The movable rod has the power of moving towards the fixed rod due to the arrangement of the first spring.

Preferably, the upper surface of the lifting block is fixedly connected with two connecting rods, and one end of each connecting rod, which is far away from the lifting block, is fixedly connected with the bottom end face of the pulling block.

Thanks to the arrangement of the connecting rod, the lifting block has the effect of moving synchronously with the pulling block.

Preferably, a through hole is formed in the middle of the lifting block in a penetrating mode, a guide column penetrates through the through hole, one end of the guide column is fixedly connected with the bottom block, and the other end of the guide column is fixedly connected with a limiting head.

Benefit from setting up of guide post and make the lift stability of elevator higher

Preferably, the guide post is equipped with the second spring near the outside cover of the one end of spacing head, the one end butt spacing head of second spring, the other end butt lifting block's of second spring upper surface.

Due to the arrangement of the second spring, the lifting block can reset after being stretched to drive the follow-up block to return to the original position to be clamped with the driving gear again.

Preferably, the side of follow-up piece is equipped with the stopper, the one end fixed connection elevator block of stopper, the other end and the follow-up piece laminating of stopper.

The arrangement of the limiting block enables one direction of the follow-up block to be limited, and the movable rod has a self-locking function.

Preferably, the rotating disc is embedded with the upper surface of the drawing box, and the upper surface of the drawing box is provided with an annular groove for embedding the rotating disc.

To sum up, the utility model discloses a technological effect and advantage: this novel adipose-derived stem cell exosome culture device, cooperation through pull box and fixture can be stable will cultivate the household utensils fixed, promote stability and independence that the cultivation household utensils were placed, movable rod and dead lever cooperation through having self-locking function, make placing of cultivation household utensils more convenient, the cultivation household utensils of different specifications all can be adjusted fixedly through the mode of one-hand operation rotary disk, it is more convenient to make the mode of resetting of movable rod through setting up of drawing the piece, the one-hand pulling draws the piece and can be with drive gear's spacing separation, fixed mode simple operation to the cultivation household utensils.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of a clamping mechanism;

FIG. 3 is a schematic view of a pulling plate structure;

fig. 4 is a schematic view of a connection structure of the pull block and the bottom block.

In the figure: 1. a device main body; 2. a clamping mechanism; 3. a box is drawn out; 4. a culture bin; 5. pulling the block; 201. a drive gear; 202. pulling a plate; 203. a fixed block; 204. a first spring; 205. rotating the disc; 206. a movable rod; 207. a fixing rod; 208. a tooth socket; 501. a connecting rod; 502. a guide post; 503. a limiting head; 504. a second spring; 505. a follow-up block; 506. a limiting block; 507. a bottom block; 508. a lifting block.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

Referring to fig. 1, a novel adipose-derived stem cell exosome culture device, including device main part 1, the inside of device main part 1 is equipped with the multilayer baffle, and the multilayer baffle is with a plurality of cultivation storehouses 4 of device main part 1 inside partition department, and the equal slidable mounting in inside of every cultivation storehouse 4 has pull box 3, and the upper surface of pull box 3 is equipped with multiunit fixture 2. The clamping mechanism 2 can stably fix the culture vessel and improve the placing stability of the culture vessel.

Referring to fig. 2 and 3, the clamping mechanism 2 includes a movable rod 206, a fixed rod 207, a pull block 5 and a rotating disc 205, the interior of the drawing box 3 is hollow, the upper surface of the drawing box 3 corresponds to the fixed rod 207, a sliding groove is horizontally arranged at the position of the movable rod 206, the bottom of the fixed rod 207 passes through the sliding groove and is fixedly connected with the sliding groove, the bottom of the movable rod 206 passes through the sliding groove and is slidably connected with the sliding groove, the bottom side wall of the movable rod 206 is fixedly connected with a pull plate 202, the pull plate 202 is horizontally arranged and is far away from one end of the sliding rod and is fixedly connected with a first spring 204, a driving gear 201 meshed with the pull plate 202 is arranged beside the pull plate 202, a tooth socket 208 meshed with the driving gear 201 is arranged on the pull plate 202, one side of the driving gear 201 far away from the pull plate 202 is clamped with a follower block 505, and the upper surface of the driving gear 201 is fixedly connected with the rotating disc 205. One end of the first spring 204 far away from the pulling plate 202 is fixedly connected with a fixed block 203, and the fixed block 203 is fixedly installed inside the drawing box 3 (shown in fig. 1). The rotating disc 205 is embedded with the upper surface of the drawing box 3, and the upper surface of the drawing box 3 is provided with an annular groove for the rotating disc 205 to be embedded. The rotating disc 205 is rotated by one hand, the rotating disc 205 drives the driving gear 201 to rotate, the driving gear 201 drives the pulling plate 202 to move by meshing with the tooth grooves 208, and then the pulling plate 202 moves towards a direction away from the fixing rod 207.

Referring to fig. 2 and 4, the follower block 505 is hinged to the lifting block 508, the lifting block 508 is elastically arranged, the bottom of the lifting block 508 is provided with a bottom block 507, a pulling block 5 is arranged above the lifting block 508, and the pulling block 5 is of a hollow rectangular structure and extends out of the upper surface of the pulling box 3. The upper surface of the lifting block 508 is fixedly connected with two connecting rods 501, and one end of each connecting rod 501 far away from the lifting block 508 is fixedly connected with the bottom end face of the pulling block 5. The through-hole has been seted up in the middle part of elevator 508 through, and the inside of through-hole has passed guide post 502, and the one end fixed connection bottom block 507 of guide post 502, the other end fixed connection of guide post 502 has stopper 503. A second spring 504 is sleeved outside one end of the guide column 502 close to the limiting head 503, the lifting block 508 moves upwards along the guide column 502, the second spring 504 is compressed, the follow-up block 505 leaves the driving gear 201, one end of the second spring 504 abuts against the limiting head 503, and the other end of the second spring 504 abuts against the upper surface of the lifting block 508. A limit block 506 is arranged beside the follow-up block 505, one end of the limit block 506 is fixedly connected with a lifting block 508, and the other end of the limit block 506 is attached to the follow-up block 505. The follower block 505 is hinged with the lifting block 508, but the follower block 505 can only swing in one direction, namely the direction that the driving gear 201 rotates to enable the movable rod 206 to leave the fixed rod 207.

The working principle is as follows: when the culture dish is used, firstly, a culture dish filled with cells is transferred towards the clamping mechanism 2, then the rotating disc 205 is rotated by one hand, the rotating disc 205 drives the driving gear 201 to rotate, the driving gear 201 drives the pulling plate 202 to move by meshing with the tooth grooves 208, further, the pulling plate 202 moves towards the direction far away from the fixing rod 207, the first spring 204 is compressed, the movable rod 206 is far away from the fixing plate, when the culture dish is not placed enough by rotating the rotating disc 205 for one circle, the rotating disc 205 can be loosened to continue rotating, the first spring 204 cannot reset when the rotating disc 205 is loosened, so that the movable rod 206 has a self-locking effect, and the specific self-locking principle is as follows:

when the driving gear 201 rotates, the following block 505 located inside the tooth block of the driving gear 201 is forced to swing, the following block 505 is hinged with the lifting block 508, but the following block 505 can only swing in one direction, that is, the driving gear 201 rotates to enable the movable rod 206 to leave the direction of the fixed rod 207, when the rotating disk 205 is loosened, the first spring 204 resets to push the pulling plate 202 back to the original position, but since the tooth grooves 208 are meshed with the driving gear 201, the driving gear 201 is limited by the following block 505 and cannot rotate, the reset elastic force of the first spring 204 cannot reset the movable rod 206, so that the movable rod 206 can be automatically locked after moving until the movable rod 206 is moved to a position a culture vessel between the fixed rod 207 and the movable rod 206, after the culture vessel is placed, the pulling block 5 is pulled, the pulling block 5 drives the lifting block 508 to move up along the guide column 502 through the connecting rod 501, the second spring 504 is compressed, the following block 505 leaves the driving gear 201, the driving gear 201 can rotate, and the first spring 204 resets to drive the pulling plate 202 to move, the movable rod 206 moves towards the fixed rod 207, so that the culture vessel can be clamped in the main body of the culture vessel clamping device 1, and the culture vessel has good stability.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. A novel adipose-derived stem cell exosome culture device comprises a device main body (1) and is characterized in that a plurality of layers of partition plates are arranged inside the device main body (1), the multilayer partition plates divide the inside of the device main body (1) into a plurality of culture bins (4), a drawing box (3) is slidably mounted inside each culture bin (4), and a plurality of groups of clamping mechanisms (2) are arranged on the upper surface of each drawing box (3);

the clamping mechanism (2) comprises a movable rod (206), a fixed rod (207), a pulling block (5) and a rotating disc (205), the interior of the drawing box (3) is hollow, a sliding groove is horizontally formed in the position, corresponding to the fixed rod (207) and the movable rod (206), of the upper surface of the drawing box (3), the bottom of the fixed rod (207) penetrates through the sliding groove and is fixedly connected with the sliding groove, the bottom of the movable rod (206) penetrates through the sliding groove and is in sliding connection with the sliding groove, a pulling plate (202) is fixedly connected to the side wall of the bottom end of the movable rod (206), a first spring (204) is fixedly connected to one end, far away from the sliding rod, of the pulling plate (202) in a horizontal arrangement mode, a driving gear (201) meshed with the pulling plate is arranged on the side of the pulling plate (202), a tooth groove (208) meshed with the driving gear (201) is formed in the pulling plate (202), one side, far away from the pulling plate (202), is clamped with a follow-up block (505), and the upper surface of the driving gear (201) is fixedly connected with the rotating disc (205);

the follow-up block (505) is hinged with a lifting block (508), the lifting block (508) is elastically arranged, the bottom of the lifting block (508) is provided with a bottom block (507), a pulling block (5) is arranged above the lifting block (508), the pulling block (5) is of a hollow rectangular structure, and extends out of the upper surface of the drawing box (3).

2. The novel adipose-derived stem cell exosome culture device according to claim 1, characterized in that one end of the first spring (204) far away from the pulling plate (202) is fixedly connected with a fixing block (203), and the fixing block (203) is fixedly installed inside the pulling box (3).

3. The novel adipose-derived stem cell exosome culture device according to claim 1, characterized in that two connecting rods (501) are fixedly connected to the upper surface of the lifting block (508), and one ends of the two connecting rods (501) far away from the lifting block (508) are fixedly connected to the bottom end face of the pulling block (5).

4. The novel adipose-derived stem cell exosome culture device according to claim 3, wherein a through hole is formed in the middle of the lifting block (508), a guide column (502) penetrates through the through hole, one end of the guide column (502) is fixedly connected with a bottom block (507), and the other end of the guide column (502) is fixedly connected with a limiting head (503).

5. The novel adipose-derived stem cell exosome culture device according to claim 4, characterized in that a second spring (504) is sleeved outside one end of the guide column (502) close to the limiting head (503), one end of the second spring (504) abuts against the limiting head (503), and the other end of the second spring (504) abuts against the upper surface of the lifting block (508).

6. The novel adipose-derived stem cell exosome culture device according to claim 1, wherein a limiting block (506) is arranged beside the follow-up block (505), one end of the limiting block (506) is fixedly connected with the lifting block (508), and the other end of the limiting block (506) is attached to the follow-up block (505).

7. The novel adipose-derived stem cell exosome culture device according to claim 1, wherein the rotating disc (205) is embedded in the upper surface of the drawing box (3), and the upper surface of the drawing box (3) is provided with an annular groove for the rotating disc (205) to be embedded in.