CN217895570U - Fixed and mixing device of over-and-under type suspension cell culture bag - Google Patents
Fixed and mixing device of over-and-under type suspension cell culture bag Download PDFInfo
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- CN217895570U CN217895570U CN202222275521.0U CN202222275521U CN217895570U CN 217895570 U CN217895570 U CN 217895570U CN 202222275521 U CN202222275521 U CN 202222275521U CN 217895570 U CN217895570 U CN 217895570U
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- fixedly connected
- cell culture
- culture bag
- placing
- mixing device
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- 238000004113 cell culture Methods 0.000 title claims abstract description 46
- 239000000725 suspension Substances 0.000 title claims abstract description 36
- 239000011435 rock Substances 0.000 abstract description 8
- 238000000034 method Methods 0.000 description 8
- 239000001963 growth medium Substances 0.000 description 5
- 238000012258 culturing Methods 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000003139 buffering effect Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000004114 suspension culture Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 230000035939 shock Effects 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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Abstract
The utility model provides a pair of fixed and mixing device of over-and-under type suspension cell culture bag, including the base, department fixed mounting has positive reverse motor in the middle of the upper surface of base, the output fixedly connected with hob of positive reverse motor, and the upper surface top fixed mounting of bracing piece has square supporting shoe, and department all is provided with first notch in the middle of the inner wall of square supporting shoe, and spacing inside left side fixed mounting has spring telescopic rod, and the equal fixed mounting in both sides of square supporting shoe has the aseptic box of placing. The utility model provides a pair of fixed and mixing device of over-and-under type suspension cell culture bag, a plurality of second notch swing joint through the inside setting of square supporting shoe has a plurality of aseptic boxes of placing, realizes multiple cell simultaneous culture, starts positive and negative motor and makes the aseptic cell culture bag of placing the box inside evenly rock, has practiced thrift the manpower, and after cell culture work, aseptic box of placing can take out through the fixed plate, is convenient for wash.
Description
Technical Field
The utility model relates to a cell culture and transfer technical field especially relate to a fixed and mixing device of over-and-under type suspension cell culture bag.
Background
Suspension culture refers to a tissue culture system for culturing single cells and small cell clusters in a liquid culture medium which is constantly stirred or shaken, and is a culture mode of anchorage-independent cells, some anchorage-dependent cells can be cultured by the method after adaptation and selection, the scale of suspension culture is relatively simple to increase, the volume can be increased only, the depth exceeds an index, the culture medium needs to be stirred, and air needs to be introduced into the culture medium in a deep layer to ensure sufficient gas exchange.
The method of culturing the isolated plant tissue by using the solid agar medium has been widely used in plant genetic experiments, but the method has some disadvantages in some aspects, such as that the nutrient components of the plant callus in the growth process and the metabolites produced by the plant tissue present a gradient distribution in the culturing process, and the agar itself has some unknown material components which may affect the culture.
However, the existing general operation process is to transfer the undifferentiated callus into the liquid culture medium for culture, when culturing various cells, the cells need to be placed in the culture medium in batches for culture, the speed is slow, and the process of collecting the cells in the suspension cell bag needs to be artificially mixed and controlled, which wastes time and labor,
therefore, it is necessary to provide a lifting suspension cell culture bag fixing and mixing device to solve the above technical problems.
SUMMERY OF THE UTILITY MODEL
The utility model provides a fixed and mixing device of over-and-under type suspension cell culture bag has solved current cell culture device and can not carry out multiple cell suspension culture simultaneously, and the cell in the suspension cell bag is collected and is needed artificial mixing and control, the problem that wastes time and energy.
In order to solve the technical problem, the utility model provides a pair of fixed and mixing device of over-and-under type suspension cell culture bag, include: the base, department fixed mounting has just reverse motor in the middle of the upper surface of base, the output fixedly connected with hob of just reverse motor, the surperficial movable sleeve of hob has nut cover, the first spacing of right side fixedly connected with of just reverse motor, nut cover's last fixed surface installs the bracing piece, the last surface top fixed mounting of bracing piece has square supporting shoe, department all is provided with first notch in the middle of square supporting shoe's the inner wall, square supporting shoe's the inside upper right corner fixed mounting has the spacing of second, spacing inside left side fixed mounting has spring telescopic rod, spring telescopic rod's left side fixedly connected with arm-tie, the last fixed surface of arm-tie is connected with the pull rod, the left side fixedly connected with fixture block of arm-tie, the equal fixed mounting in both sides of square supporting shoe has the aseptic box of placing, aseptic box inner wall fixedly connected with blotter of placing, the aseptic box of placing has placed the cell culture bag inside.
The middle of the square supporting block is provided with a sleeve hole which is convenient to sleeve on the surface of the sleeve, and a clamping block on the left side of the pulling plate is clamped in a clamping groove on the right side of the fixing plate through the spring telescopic rod.
Preferably, the square supporting block is fixedly connected to the surface of the supporting rod through a sleeve, the sleeve is fixedly connected to the surface of the supporting rod through a first fixing disc at the bottom, and a second fixing disc is fixedly connected to the top end of the supporting rod.
Preferably, the upper right corner of the sterile placing box is fixedly connected with a connecting plate, and the right side of the connecting plate is fixedly connected with a fixing plate.
Preferably, the left side of the inner wall of the sterile placing box is provided with a second notch, the upper right corner of the sterile placing box is fixedly and movably connected with a cover plate through a connecting rod and a rotary drum, and the left side of the cover plate is movably connected with a first threaded nail.
The cover plate is fixedly clamped in the second notch through the first threaded nail.
Preferably, a pull ring is fixedly connected to the middle of the upper surface of the cover plate, a buffer plate is fixedly connected to the bottom of the cover plate, a plurality of buffer springs are fixedly arranged at the bottom of the buffer plate from left to right, and fixing pads are fixedly connected to the bottoms of the buffer springs.
Preferably, the sterile placing box is movably placed on the surface of the placing plate through the lifting and contracting rods on two sides of the upper surface of the base.
Compared with the prior art, the utility model provides a pair of fixed and mixing device of over-and-under type suspension cell culture bag has following beneficial effect:
the utility model provides a fixed and mixing device of over-and-under type suspension cell culture bag, a plurality of second notch swing joint through the inside setting of square supporting shoe has a plurality of aseptic boxes of placing, realizes multiple cell simultaneous culture, starts positive and negative motor simultaneously and makes the aseptic box of placing inside cell culture bag evenly rock, has practiced thrift manpower and time, and after cell culture work, aseptic box of placing can take out through the fixed plate, is convenient for wash.
Drawings
Fig. 1 is a schematic structural diagram of a first embodiment of a lifting suspension cell culture bag fixing and mixing device provided by the present invention;
FIG. 2 is an enlarged view of a portion A of FIG. 1;
FIG. 3 is a top view of the lifting suspension cell culture bag fixing and mixing device provided by the present invention;
FIG. 4 is an elevated view of the present aseptic culture cassette;
fig. 5 is a schematic structural diagram of a second embodiment of the lifting suspension cell culture bag fixing and mixing device provided by the present invention.
Reference numbers in the figures: 1. the base, 2, the motor just reverses, 3, the hob, 4, the nut cover, 5, first spacing frame, 6, the bracing piece, 7, first fixed disk, 8, the second fixed disk, 9, the sleeve, 10, square supporting shoe, 11, first notch, 12, the fixed plate, 13, the second spacing frame, 14, the spring telescopic link, 15, the pull rod, 16, the arm-tie, 17, the fixture block, 18, the connecting plate, 19, the aseptic box of placing, 20, the second notch, 21, the blotter, 22, the connecting rod, 23, the rotary drum, 24, the apron, 25, first screw thread nail, 26, the pull ring, 27, the buffer plate, 28, buffer spring, 29, the fixed bolster, 30, the cell culture bag, 31, place the board, 32, the liter pole that contracts, 33, the suspension board, 34, U type cardboard, 35, the second screw thread nail.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and embodiments.
First embodiment
Please refer to fig. 1, fig. 2, fig. 3, and fig. 4, wherein fig. 1 is a schematic structural diagram of a first embodiment of a lifting suspension cell culture bag fixing and mixing device according to the present invention; FIG. 2 is an enlarged view of a portion A of FIG. 1; FIG. 3 is a top view of the lifting suspension cell culture bag fixing and mixing device provided by the present invention; FIG. 4 is an elevation view of the present aseptic culture cassette. The utility model provides a fixed and mixing device of over-and-under type suspension cell culture bag includes: base 1, department fixed mounting has just reversing motor 2 in the middle of base 1's the upper surface, just reversing motor 2's output fixedly connected with hob 3, the surperficial movable sleeve of hob 3 has nut cover 4, just reversing motor 2's the first spacing 5 of right side fixedly connected with, nut cover 4's last fixed mounting has bracing piece 6, bracing piece 6's upper surface top fixed mounting has square supporting shoe 10, department all is provided with first notch 11 in the middle of square supporting shoe 10's the inner wall, square supporting shoe 10's inside upper right corner fixed mounting has second spacing 13, spacing 13's inside left side fixed mounting has spring telescopic link 14, spring telescopic link 14's left side fixedly connected with arm-tie 16, arm-tie 16's last fixed connection has pull rod 15, arm-tie 16's left side fixedly connected with fixture block 17, the equal fixed mounting in both sides of square supporting shoe 10 has the aseptic box 19 of placing, aseptic box 19 inner wall fixedly connected with blotter 21 of placing, aseptic box 19 has placed inside cell culture bag 30.
The middle of the square supporting block 10 is provided with a sleeve hole which is convenient to be sleeved on the surface of the sleeve 9, and a clamping block 17 on the left side of the pulling plate 16 is clamped in a clamping groove on the right side of the fixing plate 12 through a spring telescopic rod 14.
The square supporting block 10 is fixedly connected to the surface of the supporting rod 6 through a sleeve 9, the sleeve 9 is fixedly connected to the surface of the supporting rod 6 through a first fixing plate 7 at the bottom, and a second fixing plate 8 is fixedly connected to the top end of the supporting rod 6.
The upper right corner of the aseptic box 19 is fixedly connected with a connecting plate 18, and the right side of the connecting plate 18 is fixedly connected with a fixing plate 12.
The left side of the inner wall of the sterile placing box 19 is provided with a second notch 20, the upper right corner of the sterile placing box 19 is fixedly and movably connected with a cover plate 24 through a connecting rod 22 and a rotary drum 23, and the left side of the cover plate 24 is movably connected with a first threaded nail 25.
The cover plate 24 is fixedly clamped in the second notch 20 by the first screw 25.
The middle of the upper surface of the cover plate 24 is fixedly connected with a pull ring 26, the bottom of the cover plate 24 is fixedly connected with a buffer plate 27, the bottom of the buffer plate 27 is fixedly provided with a plurality of buffer springs 28 from left to right, and the bottom of the buffer springs 28 is fixedly connected with a fixing pad 29.
The aseptic placing box 19 is movably placed on the surface of the placing plate 31 by the telescopic bars 32 at both sides of the upper surface of the base 1.
The utility model provides a pair of fixed and mixing device's of over-and-under type suspension cell culture bag theory of operation as follows: when using, put into aseptic box 19 of placing with cell culture bag 30, pulling pull rod 15, the left fixture block 17 card of arm-tie 16 is in the draw-in groove of aseptic fixed plate 12 who places box 19 right side, start positive reverse motor 2 simultaneously, make the bracing piece 6 horizontal slip of 3 upper surfaces of hob that positive reverse motor 2 output is connected, make the aseptic box 19 of placing of square supporting shoe 10 both sides rock about, and then aseptic cell culture bag 30 of placing box 19 inside evenly rocks, the buffer board 27 of apron 24 bottom, buffer spring 28, fixing pad 29 fixes the buffering to the cell culture bag 30 who rocks simultaneously, after work, the supplementary aseptic box 19 of placing of liter shrink pole 32 takes out from square supporting shoe 10, the staff of being convenient for washs.
Compared with the prior art, the utility model provides a pair of fixed and mixing device of over-and-under type suspension cell culture bag has following beneficial effect:
through the inside a plurality of aseptic boxes 19 of placing of a plurality of second notch 20 swing joint that set up of square support block 10, realize multiple cell simultaneous culture, start positive and negative motor 2 simultaneously and make the aseptic inside cell culture bag 30 of placing box 19 evenly rock, practiced thrift manpower and time, and after cell culture work, aseptic box 30 of placing can take out the washing of being convenient for through the assistance of liter shrinkage pole 32.
Second embodiment
Referring to fig. 5, fig. 5 is a schematic structural view of a second embodiment of the lifting suspension cell culture bag fixing and mixing device according to the present invention. Based on the fixed and mixing device of over-and-under type suspension cell culture bag that the first embodiment of this application provided, the second embodiment of this application provides another kind of fixed and mixing device of over-and-under type suspension cell culture bag. The second embodiment is only a preferred mode of the first embodiment, and the implementation of the second embodiment does not affect the implementation of the first embodiment alone.
Specifically, the difference that a fixed and mixing device of over-and-under type suspension cell culture bag that the second embodiment of this application provided lies in, a fixed and mixing device of over-and-under type suspension cell culture bag, the fixed fixedly connected with suspension plate 33 in bottom of apron 24, the right side of suspension plate 33 passes through second screw 35 fixedly connected with U type cardboard 34.
The right side of the suspension plate 33 is provided with a sleeve hole, and the second threaded nail 35 penetrates through the sleeve hole and is clamped in a clamping groove in the right side of the U-shaped clamping plate 34.
The working principle is as follows: the second screw 35 on the right side that the suspension board 33 passes through is fixedly connected with U-shaped cardboard 34, carries out secondary shock attenuation buffering to the cell culture bag 30 that rocks.
Has the beneficial effects that: second screw 35 fixedly connected with U type cardboard 34 on right side that the suspended plate 33 passes through carries out secondary shock attenuation buffering to the cell culture bag 30 that rocks, can make things convenient for the later stage to dismantle simultaneously and wash the change.
The above only is the embodiment of the present invention, not limiting the scope of the present invention, all the equivalent structures or equivalent processes of the present invention are used in the specification and the attached drawings, or directly or indirectly applied to other related technical fields, and the same principle is included in the protection scope of the present invention.
Claims (6)
1. The utility model provides a fixed and mixing device of over-and-under type suspension cell culture bag which characterized in that includes: the base, department fixed mounting has just reverse motor in the middle of the upper surface of base, the output fixedly connected with hob of just reverse motor, the surperficial movable sleeve of hob has nut cover, the first spacing of right side fixedly connected with of just reverse motor, nut cover's last fixed surface installs the bracing piece, the last surface top fixed mounting of bracing piece has square supporting shoe, department all is provided with first notch in the middle of square supporting shoe's the inner wall, square supporting shoe's the inside upper right corner fixed mounting has the spacing of second, spacing inside left side fixed mounting has spring telescopic rod, spring telescopic rod's left side fixedly connected with arm-tie, the last fixed surface of arm-tie is connected with the pull rod, the left side fixedly connected with fixture block of arm-tie, the equal fixed mounting in both sides of square supporting shoe has the aseptic box of placing, aseptic box inner wall fixedly connected with blotter of placing, the aseptic box of placing has placed the cell culture bag inside.
2. The lifting type suspension cell culture bag fixing and uniformly mixing device according to claim 1, wherein the square supporting block is fixedly connected to the surface of the supporting rod through a sleeve, the sleeve is fixedly connected to the surface of the supporting rod through a first fixing disk at the bottom, and a second fixing disk is fixedly connected to the top end of the supporting rod.
3. The lifting type suspension cell culture bag fixing and uniformly mixing device according to claim 1, wherein a connecting plate is fixedly connected to the upper right corner of the sterile placing box, and a fixing plate is fixedly connected to the right side of the connecting plate.
4. The lifting type suspension cell culture bag fixing and uniformly mixing device according to claim 1, wherein a second notch is formed in the left side of the inner wall of the sterile placing box, a cover plate is fixedly and movably connected to the upper right corner of the sterile placing box through a connecting rod and a rotating cylinder, and a first threaded nail is movably connected to the left side of the cover plate.
5. The lifting type suspension cell culture bag fixing and uniformly mixing device according to claim 4, wherein a pull ring is fixedly connected to the middle of the upper surface of the cover plate, a buffer plate is fixedly connected to the bottom of the cover plate, a plurality of buffer springs are fixedly arranged at the bottom of the buffer plate from left to right, and a fixing pad is fixedly connected to the bottom of each buffer spring.
6. The lifting type suspension cell culture bag fixing and uniformly mixing device according to claim 1, wherein the sterile placing box is movably placed on the surface of the placing plate through telescopic rods on two sides of the upper surface of the base.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222275521.0U CN217895570U (en) | 2022-08-29 | 2022-08-29 | Fixed and mixing device of over-and-under type suspension cell culture bag |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222275521.0U CN217895570U (en) | 2022-08-29 | 2022-08-29 | Fixed and mixing device of over-and-under type suspension cell culture bag |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217895570U true CN217895570U (en) | 2022-11-25 |
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ID=84107189
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202222275521.0U Expired - Fee Related CN217895570U (en) | 2022-08-29 | 2022-08-29 | Fixed and mixing device of over-and-under type suspension cell culture bag |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217895570U (en) |
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2022
- 2022-08-29 CN CN202222275521.0U patent/CN217895570U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20221125 |
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| CF01 | Termination of patent right due to non-payment of annual fee |