CN217895485U

CN217895485U - Protein purification chip

Info

Publication number: CN217895485U
Application number: CN202221008623.XU
Authority: CN
Inventors: 赵钢; 李子样; 陈娜娜
Original assignee: Changzhou Juxin Biotechnology Co ltd; Henan Yikai Chuangtuo Technology Co ltd; Suzhou Century Coordinate Biotechnology Co ltd
Current assignee: Changzhou Juxin Biotechnology Co ltd; Henan Yikai Chuangtuo Technology Co ltd; Suzhou Century Coordinate Biotechnology Co ltd
Priority date: 2022-04-24
Filing date: 2022-04-24
Publication date: 2022-11-25
Anticipated expiration: 2032-04-24

Abstract

The utility model provides a protein purification chip, including the chip body, wherein, the chip body sets up a plurality of purification reaction tanks, connects through micro-fluidic channel between each purification reaction tank, and the purification reaction tank that is located one end is connected with feed liquor channel, and the purification reaction tank and the play liquid tank of the other end are connected. The invention adopts the micro-fluidic chip technology, utilizes the micro-channel to connect a plurality of purification reaction tanks, and purifies through a plurality of reaction purification tanks, thereby purifying the target protein to the utmost extent.

Description

Protein purification chip

Technical Field

The utility model relates to a protein purification technical field, in particular to protein purification chip.

Background

Protein purification uses the inherent similarity and difference between different proteins, uses the similarity between various proteins to remove contamination of non-protein substances, and uses the difference between proteins to purify a target protein from other proteins. The size, shape, charge, hydrophobicity, solubility and biological activity of each protein can vary, and the protein can be extracted from a mixture such as E.coli lysate to obtain recombinant protein.

The purification of proteins is roughly divided into two stages, a coarse separation stage and a fine purification stage. The method used for purifying the protein is a resin method. The crude separation stage mainly separates the target protein from other cell components such as DNA, RNA and the like, and the sample has large volume and components, so the resin used has high capacity, high flow rate, large particles and wide particle size distribution, can quickly separate the protein from pollutants, and can add corresponding protective agents (such as protease inhibitors) if necessary to prevent the target protein from being degraded. The fine purification stage needs higher resolution, and the stage is to distinguish the target protein from those with molecular weight and physical and chemical properties close to each other, to use smaller resin particles to improve the resolution, and to use ion exchange columns and hydrophobic columns, which are used in combination with two factors of resin selectivity and column efficiency. The selectivity refers to the specificity of the combination of the resin and the target protein, the column effect refers to the capability of intensively eluting each protein component from the resin one by one, and the narrower the elution peak, the better the column effect. Only good selectivity, too broad elution peak, and inefficient protein separation as it is.

At present, protein purification mainly takes purification resin as main material, and the purification efficiency is low.

SUMMERY OF THE UTILITY MODEL

The object of the utility model is to solve at least one of the technical defects.

Therefore, an object of the present invention is to provide a protein purification chip to solve the above-mentioned problems and overcome the disadvantages of the prior art.

In order to achieve the above object, an embodiment of an aspect of the present invention provides a protein purification chip, including the chip body, wherein, the chip body sets up a plurality of purification reaction tanks, connects through micro-fluidic channel between each purification reaction tank, and the purification reaction tank that is located one end is connected with inlet channel, and the purification reaction tank and the drain tank of the other end are connected.

Preferably, the microfluidic channel is in a trapezoidal cylinder structure.

In any of the above embodiments, the purification reaction tank is surface-treated, and a material for purification is attached to the purification reaction tank, wherein the material for purification is nickel ions capable of purifying a protein containing an HIS tag.

Preferably, in any of the above schemes, the chip body has a length of 10cm, a width of 5cm and a height of 0.25cm.

Preferably, in any of the above schemes, the chip body is made of a PDMS material.

Preferably, in any of the above schemes, the chip body introduces the sample liquid to be purified through the liquid inlet slot, the sample liquid is pressurized by the air compression pump to form a certain pressure, and the sample liquid enters the purification reaction slots through the microfluidic channel, passes through each purification reaction slot, and is finally led out through the liquid outlet slot.

Preferably, in any of the above embodiments, the purification reaction tanks are arranged in five groups.

Compared with the prior art, the utility model has the advantages and beneficial effects do:

the invention adopts the micro-fluidic chip technology, utilizes the micro-channel to connect a plurality of purification reaction tanks, and purifies through a plurality of reaction purification tanks, thereby purifying the target protein to the maximum extent.

Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.

Drawings

The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:

FIG. 1 is a schematic diagram of a protein purification chip according to an embodiment of the present invention;

FIG. 2 is a side view of FIG. 1 of a protein purification chip according to an embodiment of the present invention.

Wherein: 1. a liquid inlet channel; 2. a purification reaction tank; 3. a microfluidic channel; 4. and a liquid outlet groove.

Detailed Description

Reference will now be made in detail to the embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.

In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those of ordinary skill in the art.

As shown in fig. 1, the utility model provides a protein purification chip, including the chip body, wherein, the chip body sets up a plurality of purification reaction tanks 2, connects through micro-fluidic channel 3 between each purification reaction tank 2, and the purification reaction tank 2 that is located one end is connected with inlet channel 1, and the purification reaction tank 2 and the play liquid tank 4 of the other end are connected, micro-fluidic channel 3 is trapezoidal cylinder structure, purification reaction tank 2 is through surface treatment, adheres to the material for purification, and this purification material is nickel ion, nickel ion can purify the protein that contains the HIS label, chip body length 10cm, wide 5cm, high 0.25cm, the chip body adopts the PDMS material to make, the chip body is leading-in through the feed liquor groove sample liquid that will prepare the purification, pressurizes through the air compression pump, forms certain pressure, and sample liquid passes through micro-fluidic channel 3, enters into purification reaction tank 2, through each purification reaction tank 2, finally leads out through going out liquid tank 4, purification reaction tank 2 sets up to five groups.

The utility model discloses a use method:

for example, the chip purification reaction tank is coated with nickel ions to purify the protein containing the HIS tag.

Balance liquid: 20mM PB,50mM Tris-HCl,500mM NaCl,5% imidazole, pH 7.4

Washing liquid: 20mM PB,50mM Tris-HCl,500mM NaCl,10% imidazole, pH 7.4

Eluent: 20mM PB,50mM Tris-HCl,500mM NaCl,25% imidazole, pH 7.4

Through inlet channel 1, add balanced liquid earlier, utilize the air compressor pump, leading-in liquid forms certain velocity of flow (0.5 ml/15 second velocity of flow) and balances the purification reaction tank that contains nickel ion, and after balanced liquid derives from the drain tank, the liquid feeding lasts 2 minutes.

Adding a buffer solution containing a sample into the balanced chip through an air compression pump;

after the sample loading is finished, after the sample buffer solution completely flows out, washing with 10 times of washing solution, and repeating for three times for removing impurity proteins;

eluting with 3 times of eluent, eluting the target protein adsorbed by the chip purification reaction tank, and recovering.

In the description of the present specification, reference to the description of "one embodiment," "some embodiments," "an example," "a specific example," or "some examples" or the like means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

It will be understood by those skilled in the art that the invention, including any combination of the elements of the above description and the detailed description and illustrated in the accompanying drawings, is not limited to the details and should not be construed as limited to the embodiments set forth herein for the sake of brevity. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Although embodiments of the present invention have been shown and described, it is to be understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that changes, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art without departing from the principles and spirit of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The protein purification chip comprises a chip body and is characterized in that the chip body is provided with a plurality of purification reaction grooves, the purification reaction grooves are connected through a microfluidic channel, the purification reaction groove at one end is connected with a liquid inlet channel, and the purification reaction groove at the other end is connected with a liquid outlet groove.

2. The protein purification chip of claim 1, wherein the microfluidic channel has a trapezoidal cylindrical structure.

3. The protein purification chip of claim 1, wherein the chip body has a length of 10cm, a width of 5cm and a height of 0.25cm.

4. The protein purification chip of claim 1, wherein the chip body is made of PDMS material.

5. The protein purification chip according to claim 1, wherein the purification reaction chambers are arranged in five groups.