CN217868875U

CN217868875U - Small-size portable pine wood nematode quick-detection device

Info

Publication number: CN217868875U
Application number: CN202221939142.0U
Authority: CN
Inventors: 陈佳瑶; 郑磊
Original assignee: Anhui Shenghai Ecological Technology Co ltd
Current assignee: Anhui Shenghai Ecological Technology Co ltd
Priority date: 2022-07-26
Filing date: 2022-07-26
Publication date: 2022-11-22
Anticipated expiration: 2032-07-26

Abstract

The utility model relates to a nematode disease detects technical field, and discloses small-size portable pine wood nematode disease quick-witted fast detection device, the collecting box comprises a collection box, the inside right side of collecting box has been seted up and has been placed the chamber, the internally mounted who places the chamber has lifting unit, through the inside with the reagent box is put into to the kit, then put into the inside of freezing groove with the ice-cube, the export of freezing groove is stopped up to the reinsertion rubber buffer, the inside that the reagent box transmitted to the reagent box is passed through in the air conditioning transmission through the ice-cube release, accomplish the purpose of preserving the reagent box, then place the pine wood nematode disease detection machine at the top of fixed plate, then drive the lead screw through rotating the hand and rotate, the lead screw rotates through the thread piece, the fixed plate drives the inside that pine wood nematode disease detection machine got into the collecting box, thereby with the same purpose of collecting of pine wood nematode disease detection machine and reagent box, then the staff moves the collecting box through the handle and removes, reach the purpose of protection to pine wood nematode disease detection machine in handling simultaneously.

Description

Small-size portable pine wood nematode quick-detection device

Technical Field

The application relates to the technical field of nematode disease detection, in particular to a small-size portable fast pine wood nematode disease detection device.

Background

Pine wilt disease, also known as pine wilt disease, is a destructive forest disease caused by pine nematodes, belongs to a major foreign invasive species in China, has been listed as an object for quarantine of forest plants inside and outside China, is rapidly spread since the disease is introduced into China in 1982, has occurred in 14 provinces (cities and regions) in China and has an area of 7.7 million hm & lt 2 & gt, causes a large number of pine withers, and causes serious damage to pine resources, natural landscapes and ecological environments in China, thereby causing serious economic and ecological losses.

At present, in the process of treating trees, pine wood nematode disease detection needs to be carried out on trees through device detection to judge whether the trees are pine wood nematode disease or not, but the pine wood nematode disease detection device needs to carry out the processes of pine wood nematode separation, DNA extraction, constant-temperature amplification PCR, test paper detection and the like in the detection process, but in the process, various devices need to be used, in order to conveniently and rapidly identify whether the trees are suffered from the pine wood nematode disease, the devices need to be carried into a tree forest by workers to identify the trees, and due to the fact that the devices are multiple, the workers are not convenient to carry the trees in the tree forest, and time and labor are wasted.

SUMMERY OF THE UTILITY MODEL

In order to solve the problems proposed above, the present application provides a small-sized portable fast detection device for pine wilt disease.

The application provides a small-size portable pine wood nematode disease fast detects device adopts following technical scheme:

the small-sized portable fast detection device for the pine wilt disease comprises a collecting box, wherein a placing cavity is formed in the right side in the collecting box, a lifting assembly is arranged in the placing cavity, a fixing plate is arranged in the lifting assembly, a pine wilt disease detection machine is fixedly connected in the fixing plate, a cover plate is arranged at the top of the pine wilt disease detection machine, a freezing groove is formed in the left side in the collecting box, a heat insulation material is arranged on the inner wall of the freezing groove and is made of a glass cotton plate material, a rubber plug is inserted into the top of the freezing groove, ice blocks are placed in the freezing groove, a reagent box is fixedly connected in the freezing groove, an aluminum alloy material is used in the reagent box, a drain pipe is fixedly connected to the bottom of the freezing groove, and a sealing plate is arranged at the top of the reagent box;

the equal fixedly connected with of left and right side of collecting box links up the fishplate bar, the inside sliding connection of linking up the fishplate bar has the slide bar, the inside threaded connection of slide bar has the threaded rod, the bottom fixedly connected with of threaded rod supports a section of thick bamboo, the inside threaded connection who supports a section of thick bamboo has the third fixed pin, and the surface of third fixed pin and the inside threaded connection who links up the fishplate bar, the top fixedly connected with limiting plate of slide bar, the top fixedly connected with handle of slide bar, the positive fixedly connected with collection box of collecting box.

Through adopting above-mentioned technical scheme, put the inside of reagent box with the kit, then put the inside of freezing groove with the ice-cube, the export of freezing groove is plugged up to the reinsertion rubber buffer, the inside of reagent box is transmitted through the cold air transmission of ice-cube release, accomplish the purpose to the kit is preserved, then place the pine wood nematode disease detection machine at the top of fixed plate, then drive the inside that the pine wood nematode disease detection machine got into the collecting box through the lift group, thereby with the same purpose of collecting of pine wood nematode disease detection machine and kit, then the staff moves the collecting box through the handle and removes, reach the purpose to the protection of pine wood nematode disease detection machine in handling simultaneously.

Preferably, the lifting assembly comprises a screw rod, the outer surface of the screw rod is rotatably connected with the inside of the collecting box, one end of the screw rod, which extends to the outer side of the collecting box, is fixedly connected with a rotating handle, the outer surface of the screw rod is in spiral transmission connection with a threaded block, the inside of the threaded block is fixedly connected with the outer surface of the fixing plate, and one side, away from the threaded block, of the fixing plate is slidably connected with a positioning rod.

Through adopting above-mentioned technical scheme, rotate the commentaries on classics hand and drive the lead screw and rotate, the lead screw rotates and drives the fixed plate upward movement through the thread block, and fixed plate upward movement promotes the pine wood nematode disease detection machine and breaks away from the inside of collecting box.

Preferably, a fixing groove matched with the third fixing pin in size is formed in the left side of the limiting plate, a threaded hole matched with the third fixing pin in size is formed in the connecting plate, and the threaded hole and the fixing groove are located on the same horizontal line.

Through adopting above-mentioned technical scheme, the fixed slot all is the space that provides the third fixed pin and alternate with the screw hole.

Preferably, the cover plate is in threaded connection with a first fixing pin, the outer surface of the first fixing pin is in threaded connection with the inside of the collecting box, the sealing plate is in threaded connection with a second fixing pin, and the outer surface of the second fixing pin is in threaded connection with the inside of the collecting box.

Through adopting above-mentioned technical scheme, remove the fixed between apron and the collecting box through rotating first fixed pin, open the export of pine wood nematode disease detection machine, rotate the inside that the second fixed pin breaks away from the collecting box, remove the fixed between closing plate and the collecting box, open the export of reagent box, the staff of being convenient for takes out the kit.

To sum up, the application comprises the following beneficial technical effects:

1. the kit is placed inside the reagent box through the matching use of the reagent box, the ice blocks, the pine wood nematode disease detector, the fixing plate, the rotary handle and the screw rod, then the ice blocks are placed inside the freezing groove, the rubber plug is inserted to block the outlet of the freezing groove, cold air released by the ice blocks is transferred to the inside of the reagent box through the reagent box, the purpose of storing the kit is achieved, then the pine wood nematode disease detector is placed at the top of the fixing plate, the screw rod is driven to rotate through the rotary handle, the screw rod drives the pine wood nematode disease detector to enter the inside of the collecting box through the screw block and the fixing plate, so that the pine wood nematode disease detector and the kit are collected at the same time, then a worker moves the collecting box through the handle to move, and meanwhile, the purpose of protecting the pine wood nematode disease detector in the carrying process is achieved;

2. through the third fixed pin, support a section of thick bamboo, the slide bar, the threaded rod, the limiting plate cooperation is used, rotate the inside that the third fixed pin breaks away from a support section of thick bamboo, then the pulling supports a section of thick bamboo and drives the slide bar downstream through the threaded rod, make the slide bar drive the top that the limiting plate moved the linkage plate, then pass the inside that the limiting plate inserted the linkage plate through rotating the third fixed pin, fix the limiting plate, then rotate support a section of thick bamboo and drive the threaded rod and rotate, the threaded rod rotates the inside that breaks away from the slide bar, then according to ground flatness, adjust the length of threaded rod, thereby make the collecting box be in the level form.

Drawings

Fig. 1 is a schematic perspective view of an embodiment of the present application.

Fig. 2 is a front internal structure diagram of the application example.

Description of reference numerals: 1. a collection box; 2. a screw rod; 3. turning the hand; 4. a thread block; 5. a fixing plate; 51. positioning a rod; 6. a pine wilt disease detector; 7. a cover plate; 8. a first fixing pin; 9. a battery pack; 10. a freezing tank; 11. ice blocks; 12. a reagent box; 13. a drain pipe; 14. sealing plates; 15. a second fixing pin; 16. a connector tile; 17. a slide bar; 18. a threaded rod; 19. a support cylinder; 20. a third fixing pin; 21. a limiting plate; 22. a handle; 23. and (6) a recycling bin.

Detailed Description

The present application is described in further detail below with reference to figures 1-2.

The embodiment of the application discloses small-size portable pine wood nematode disease fast detects device. Referring to fig. 1, the small portable fast detecting device for the pine wilt disease comprises a collecting box 1, wherein a placing cavity is formed in the right side inside the collecting box 1, and a lifting assembly is arranged inside the placing cavity;

as shown in fig. 1-2, the lifting assembly comprises a screw rod 2, the outer surface of the screw rod 2 is rotatably connected with the inside of the collecting box 1, one end of the screw rod 2 extending to the outside of the collecting box 1 is fixedly connected with a rotating handle 3, so that a worker can drive the screw rod 2 to rotate through the rotating handle 3, the outer surface of the screw rod 2 is in spiral transmission connection with a threaded block 4, the inside of the threaded block 4 is fixedly connected with the outer surface of a fixing plate 5, the outer surface of the fixing plate 5 is in sliding connection with the inside of the collecting box 1, one side of the fixing plate 5, far away from the threaded block 4, is in sliding connection with a positioning rod 51, the rotating handle 3 is rotated to drive the screw rod 2 to rotate, the screw rod 2 rotates to drive the fixing plate 5 to move upwards through the threaded block 4, and the fixing plate 5 moves upwards to push the pine wood nematode disease detector 6 to be separated from the inside of the collecting box 1;

as shown in fig. 1-2, a pine wood nematode disease detector 6 is fixedly connected inside a fixing plate 5, the pine wood nematode disease detector is a commercially available product, such as a model of BX-48A, the pine wood nematode disease detector 6 ensures high detection accuracy through a matched special detection kit, a genome DNA rapid extraction reagent and a fluorescent quantitative detection reagent are contained in the kit, the detection process can be completed in one step, a rapid, sensitive and accurate method is provided for detection of pine wood nematodes, a cover plate 7 is installed on the top of the pine wood nematode disease detector 6, a first fixing pin 8 is connected to an internal thread of the cover plate 7, the outer surface of the first fixing pin 8 is connected to an internal thread of a collecting box 1, the fixing between the cover plate 7 and the collecting box 1 is released by rotating the first fixing pin 8, and an outlet of the pine wood nematode disease detector 6 is opened;

as shown in fig. 1-2, a freezing groove 10 is formed in the left side of the interior of a collecting box 1, a heat insulation material is installed on the inner wall of the freezing groove 10, the heat insulation material is made of a glass wool board material, a rubber plug is inserted into the top of the freezing groove 10, ice blocks 11 are placed inside the freezing groove 10, a reagent box 12 is fixedly connected to the interior of the freezing groove 10, a reagent box is placed inside the reagent box 12, the reagent box contains a genomic DNA rapid extraction reagent and a fluorescence quantitative detection reagent for protecting the stored reagents, the reagent box 12 is made of an aluminum alloy material, a drain pipe 13 is fixedly connected to the bottom of the freezing groove 10 for discharging melted ice blocks, a sealing plate 14 is installed on the top of the reagent box 12, a second fixing pin 15 is connected to the internal thread of the sealing plate 14, the outer surface of the second fixing pin 15 is connected to the internal thread of the collecting box 1, the second fixing pin 15 is rotated to separate from the interior of the collecting box 1, the fixing plate 14 and the collecting box 1 are released, an outlet of the reagent box 12 is opened, and a worker can take out the reagent box;

as shown in fig. 1-2, the collecting box 1 is fixedly connected with a connecting plate 16 on both left and right sides, a threaded hole matched with the size of a third fixing pin 20 is formed in the connecting plate 16, the threaded hole provides a space for the third fixing pin 20 to penetrate through, the threaded hole and the fixing groove are on the same horizontal line, the third fixing pin 20 can conveniently penetrate through the threaded hole to be inserted into the inside of the threaded groove, a sliding rod 17 is slidably connected with the inside of the connecting plate 16, a threaded rod 18 is connected with the internal thread of the sliding rod 17, the sliding rod 17 and the threaded rod 18 are used for supporting the device in a forest, the collecting box 1 becomes a workbench, a supporting cylinder 19 is fixedly connected with the bottom of the threaded rod 18, a third fixing pin 20 is connected with the internal thread of the supporting cylinder 19, the outer surface of the third fixing pin 20 is connected with the internal thread of the connecting plate 16, a limiting plate 21 is fixedly connected with the outer surface of the top of the sliding rod 17, a fixing groove matched with the size of the third fixing pin 20 is formed in the left side of the limiting plate 21, the fixing groove provides a space for the fixing pin 20 to penetrate through, a handle 22 is fixedly connected with the front side of the collecting box 1, a collecting box 23 is fixedly connected with the collecting box for recovering reagents used for the detected workers, and the centralized processing of the workers.

The implementation principle of the small-size portable pine wood nematode disease rapid detection device of the embodiment of this application is: the method comprises the steps of putting a kit into a reagent box 12, putting an ice block 11 into a freezing groove 10, inserting a rubber plug to block an outlet of the freezing groove 10, transferring cold air released by the ice block 11 into the reagent box 12 through the reagent box 12 to fulfill the purpose of storing the kit, putting a pine wood nematode disease detector 6 on the top of a fixing plate 5, driving a screw rod 2 to rotate through a rotating handle 3, driving the pine wood nematode disease detector 6 into a collecting box 1 through a thread block 4 and the fixing plate 5 by the screw rod 2 to achieve the purpose of collecting the pine wood nematode disease detector 6 and the kit, and moving the collecting box 1 through a handle 22 by a worker to move the collecting box 1 to achieve the purpose of protecting the pine wood nematode disease detector 6 in the carrying process;

furthermore, the third fixing pin 20 is rotated to be separated from the inside of the supporting cylinder 19, then the supporting cylinder 19 is pulled to drive the slide rod 17 to move downwards through the threaded rod 18, so that the slide rod 17 drives the limiting plate 21 to move to the top of the connecting plate 16, then the third fixing pin 20 is rotated to penetrate through the limiting plate 21 to be inserted into the inside of the connecting plate 16, so as to fix the limiting plate 21, then the supporting cylinder 19 is rotated to drive the threaded rod 18 to rotate, the threaded rod 18 is rotated to be separated from the inside of the slide rod 17, and then the length of the threaded rod 18 is adjusted according to the ground flatness, so that the collecting box 1 is in a horizontal shape.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and alterations may be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. Device is examined fast to small-size portable pine wood nematode disease, including collecting box (1), its characterized in that: a placing cavity is formed in the right side inside the collecting box (1), a lifting assembly is installed in the placing cavity, a fixing plate (5) is installed in the lifting assembly, a pine wilt disease detection machine (6) is fixedly connected to the inside of the fixing plate (5), a cover plate (7) is installed at the top of the pine wilt disease detection machine (6), a freezing groove (10) is formed in the left side inside the collecting box (1), an ice block (11) is placed in the freezing groove (10), a reagent box (12) is fixedly connected to the inside of the freezing groove (10), and a sealing plate (14) is installed at the top of the reagent box (12);

the equal fixedly connected with of left and right side of collecting box (1) links up fishplate bar (16), the inside sliding connection of linking up fishplate bar (16) has slide bar (17), the internal thread connection of slide bar (17) has threaded rod (18), the bottom fixedly connected with of threaded rod (18) supports a section of thick bamboo (19), the internal thread connection of a support section of thick bamboo (19) has third fixed pin (20), and the surface of third fixed pin (20) and the internal thread connection of linking up fishplate bar (16), the top external surface fixedly connected with limiting plate (21) of slide bar (17), the top fixedly connected with handle (22) of slide bar (17), the positive fixedly connected with collection box (23) of collecting box (1).

2. The small portable fast detection device for pine wilt disease of claim 1, characterized in that: the lifting assembly comprises a screw rod (2), the outer surface of the screw rod (2) is rotatably connected with the inside of the collecting box (1), a thread block (4) is connected to the outer surface of the screw rod (2) in a spiral transmission mode, the inside of the thread block (4) is fixedly connected with the outer surface of the fixing plate (5), and one side, far away from the thread block (4), of the fixing plate (5) is slidably connected with a positioning rod (51).

3. The small portable fast detection device for pine wilt disease of claim 1, characterized in that: the left side of the limiting plate (21) is provided with a fixing groove matched with the third fixing pin (20) in size, a threaded hole matched with the third fixing pin (20) in size is formed in the connecting plate (16), and the threaded hole and the fixing groove are located on the same horizontal line.

4. The small portable fast detection device for pine wilt disease of claim 1, characterized in that: the internal thread of apron (7) is connected with first fixed pin (8), the internal thread of the surface and the collecting box (1) of first fixed pin (8) is connected, the internal thread of closing plate (14) is connected with second fixed pin (15), and the internal thread of the surface and the collecting box (1) of second fixed pin (15) is connected.

5. The small portable fast detection device for pine wilt disease of claim 1, characterized in that: the inner wall of the freezing tank (10) is provided with a heat insulation material, and the inside of the reagent box (12) is made of an aluminum alloy material.