CN217868789U

CN217868789U - Orifice plate and system including same

Info

Publication number: CN217868789U
Application number: CN202221612675.8U
Authority: CN
Inventors: 李明辉; 王志凯; 王禺; 夏永恒; 张春晓; 尹进
Original assignee: Bluepha Co ltd
Current assignee: Shanghai Blue Crystal Microbial Technology Co ltd
Priority date: 2022-06-24
Filing date: 2022-06-24
Publication date: 2022-11-22
Anticipated expiration: 2032-06-24

Abstract

A pore plate and a system comprising the same relate to the technical field of instruments in synthetic biological experiments. The orifice plate includes a sidewall and a floor; the side walls and the floor form a chamber; at least one partition plate is arranged in the cavity; the partition plate divides the chamber into a plurality of sub-chambers; the bottom of the partition plate is provided with a plurality of partition plate through holes, so that all the sub-chambers are communicated with each other. A device or system comprising a well plate for use in coating or culturing. An object of the utility model is to provide a orifice plate reaches device or system including this orifice plate, both can realize that the liquid level is in same height, can reduce the technical problem that the aerosol pollutes each other again.

Description

Orifice plate and system including same

Technical Field

The utility model relates to an apparatus technical field in the synthetic biological experiment particularly, relates to a orifice plate and including the system of this orifice plate.

Background

In the field of synthetic biology, coating is a common experimental procedure. In the experimental process of gene editing, in order to detect that the target gene is successfully transferred into the receptor cell and the monoclonal antibody can be successfully picked up, a coating method is usually adopted, i.e., the bacterial liquid is uniformly coated on the surface of a solid culture medium with certain resistance and is placed under certain conditions for culture. Successfully grown cells are considered to be cells into which the gene of interest has been successfully introduced.

In the biological experiment process, the operation requirement of coating is strict, sterile materials are required in a sterile environment, and the whole operation process is complex and difficult. Meanwhile, when the number of samples in the experiment is large, the manual operation is slow, the flux requirement cannot be met, and an automatic mode is required to replace manual coating.

In the current automatic coating, a common mode is that a disposable coating head fixed at the tail end of a robot makes a track in a spiral or circular mode to realize the coating effect. It adopts a pore plate as a solid culture medium to carry out automatic coating; for example, six-well plates or one-well plates are used for automated coating. However, in the coating process, the liquid levels of the six holes of the six-hole plate are difficult to be at the same liquid level height, so that the experimental effect is influenced; the problem of aerosol mutual pollution exists in the orifice plate, has also influenced the experiment effect.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a pore plate reaches system including this pore plate, both can realize that the liquid level is in same height, can reduce the technical problem that the aerosol pollutes each other again.

In order to achieve the above object, the present invention provides the following technical solutions:

an orifice plate comprising a sidewall and a floor; the side walls and the floor form a chamber;

at least one partition plate is arranged in the cavity; the baffle plate divides the chamber into a plurality of sub-chambers;

the bottom of the partition plate is provided with a plurality of partition plate through holes, so that all the sub-chambers are communicated with each other.

In any of the above solutions, optionally, the partition through hole extends to the bottom plate.

In any of the above technical solutions, optionally, the shape of the partition through hole includes any one of a circle, an ellipse, an arc, a polygon, or a combination thereof.

In any of the above technical solutions, optionally, the shape of the partition through hole is rectangular.

In any of the above technical solutions, optionally, the partition plate includes a first partition plate and/or a second partition plate; the first partition plate is connected with the second partition plate in a crossing manner;

the number of the first partition plates is one or more;

the number of the second partition plates is one or more.

In any of the above technical solutions, optionally, the partition plates include three first partition plates and one second partition plate, where the number of the first partition plates is one;

the first partition plate is vertically and crossly connected with the second partition plate, and divides the chamber into eight sub-chambers, so that the pore plate is an eight-pore plate.

In any of the above technical solutions, optionally, three first partition plates are arranged in parallel between the side wall and the bottom plate at equal intervals, and the second partition plates are perpendicularly connected in a crossing manner on a central axis of the first partition plates;

the first partition plate includes at least one partition plate through-hole, and the second partition plate includes at least one partition plate through-hole, so that all the sub-chambers communicate with each other.

In any of the above technical solutions, optionally, the three first partition plates include a partition plate through hole respectively, and the second partition plate includes a partition plate through hole;

the partition through holes of the second partition are respectively connected with the partition through holes of the three first partitions in a mutually crossed manner, so that all the sub-chambers are mutually communicated.

In any of the above technical solutions, optionally, a longitudinal length of the partition through hole of the second partition in the length direction of the second partition is greater than a spacing distance of the three first partitions.

A system comprising a well plate as described above, said well plate being used for coating or culturing.

The beneficial effects of the utility model mainly lie in:

the utility model provides a pore plate and a system comprising the pore plate, when used for coating, the pore plate is provided with the baffle plates respectively connected with the side wall and the bottom plate, and divides the cavity into a plurality of sub-cavities, so as to avoid or reduce the problem of aerosol mutual pollution existing in the pore plate to a certain extent; the bottom through the baffle is provided with a plurality of baffle through-hole, and all sub-chambeies pass through baffle through-hole intercommunication to the liquid level that makes all sub-chambeies is in same liquid level height, has ensured the experimental effect to a certain extent.

In order to make the aforementioned and other objects, features and advantages of the present application more comprehensible, preferred embodiments accompanied with figures are described in detail below.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and that those skilled in the art can also obtain other related drawings based on these drawings without inventive efforts.

Fig. 1 is a schematic structural diagram of an orifice plate according to an embodiment of the present invention;

fig. 2 is a schematic view of another angle structure of the orifice plate according to the embodiment of the present invention;

FIG. 3 is a top view of the orifice plate shown in FIG. 2;

FIG. 4 isbase:Sub>A cross-sectional view taken along line A-A of the orifice plate shown in FIG. 2;

FIG. 5 is a left side view of the orifice plate shown in FIG. 2;

FIG. 6 is a cross-sectional view of the orifice plate shown in FIG. 5 taken along line B-B.

An icon: 110-a side wall; 120-a backplane; 130-a separator; 131-a first separator; 132-a second separator; 133-spacer through holes; 140-a sub-chamber; 150-floor support wall.

Detailed Description

To make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the attached drawings in the embodiments of the present invention are combined to clearly and completely describe the technical solution in the embodiments of the present invention, and obviously, the described embodiments are part of the embodiments of the present invention, rather than all embodiments. The components of the embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate the position or positional relationship based on the position or positional relationship shown in the drawings, or the position or positional relationship which is usually placed when the product of the present invention is used, and are only for convenience of description and simplification of the description, but do not indicate or imply that the device or element referred to must have a specific position, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another, and are not to be construed as indicating or implying relative importance.

Furthermore, the terms "horizontal", "vertical", "suspended" and the like do not imply that the components are absolutely horizontal or suspended, but may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood as a specific case by those skilled in the art.

Some embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The embodiments described below and the features of the embodiments can be combined with each other without conflict.

Examples

The embodiment provides an orifice plate and a system comprising the orifice plate; referring to fig. 1 to 6, fig. 1 is a schematic structural diagram of a hole plate provided in this embodiment; fig. 2 is a front view of the orifice plate provided in the present embodiment, fig. 3 is a plan view of the orifice plate shown in fig. 2, and fig. 5 is a left side view of the orifice plate shown in fig. 2; to more clearly show the structure, fig. 4 isbase:Sub>A sectional view taken alongbase:Sub>A-base:Sub>A of the orifice plate shown in fig. 2, and fig. 6 isbase:Sub>A sectional view taken along B-B of the orifice plate shown in fig. 5.

The well plate provided by the embodiment is applied to a coating process or a process of culturing and selecting other biological strains, such as a coating process applied to a synthetic biological experiment; for example, automatic coating operation using a three-coordinate robot; as another example, the method is applied to a strain culture operation flow.

Referring to fig. 1-6, the orifice plate includes a sidewall 110 and a bottom plate 120; the sidewalls 110 and the bottom plate 120 form a chamber; wherein the chamber is open at the top.

At least one baffle 130 is disposed within the chamber; the partition 130 is connected to the sidewall 110 and the bottom plate 120, respectively, and partitions the chamber into a plurality of sub-chambers 140; dividing the chamber into a plurality of sub-chambers 140 by baffles 130; the bottom of the partition 130 is provided with a plurality of partition through holes 133 so that all the sub-chambers 140 are communicated with each other, that is, all the sub-chambers 140 are communicated through the partition through holes 133. Through the partition plate through holes 133, the liquid level of all the sub-chambers 140 is at the same liquid level, and meanwhile, the mutual intercommunication can be ensured when the culture medium is poured, so that the culture of each hole is ensured, the problem of aerosol mutual pollution caused by the existence of a pore plate is avoided or reduced to a certain extent, the probability of cross infection is reduced, the experimental effect is ensured to a certain extent, and the working efficiency is improved.

In the alternative of this embodiment, the heights of all the partition through-holes 133 are the same or different, or the heights of some of the partition through-holes 133 are the same.

Optionally, the height of all the spacer through holes 133 is the same to facilitate the manufacturing process of the spacer through holes 133.

Optionally, the height of all the partition through holes 133 is not higher than that of the partition 130, so that the culture solution can be submerged by the height of the partition through holes 133 during coating, and the sub-chambers 140 are isolated, thereby effectively preventing cross contamination.

In an alternative of this embodiment, the partition through-holes 133 are abutted against or spaced from the bottom plate 120; optionally, the partition through hole 133 extends to the bottom plate 120, i.e. the partition through hole 133 abuts, directly connects with the bottom plate 120.

In an alternative of this embodiment, all the separator through-holes 133 have the same or different shapes, or some of the separator through-holes 133 have the same shape.

Alternatively, all the separator through-holes 133 may have the same shape to facilitate the production process of the separator through-holes 133.

In an alternative of this embodiment, the shape of the partition through-hole 133 is any one of a circle, an ellipse, an arc, a polygon, or a combination thereof; for example, it may be rectangular, circular, oval, pentagonal, hexagonal, or the like. Alternatively, the shape of the spacer through-hole 133 is rectangular.

When the orifice plate is used for coating, the partition plates 130 respectively connected with the side wall 110 and the bottom plate 120 are arranged, and the chamber is divided into a plurality of sub-chambers 140, so that the problem of aerosol mutual pollution existing in one orifice plate is avoided or reduced to a certain extent; the bottom of the partition plate 130 is provided with a plurality of partition plate through holes 133, and all the sub-chambers 140 are communicated through the partition plate through holes 133, so that the liquid levels of all the sub-chambers 140 are at the same liquid level, and the experimental effect is guaranteed to a certain extent.

In the prior art, a baffle plate is not arranged in the whole plane of a pore plate, so that aerosol can be polluted when the pore plate is used for coating; when the six-hole plate is used in the coating process, the liquid levels of the six holes are difficult to be at the same liquid level height, and the experimental effect is influenced. When the pore plate is used for coating, the advantages of the six pore plates and the one pore plate are achieved, the defects of the one pore plate and the six pore plates are avoided, aerosol pollution can be avoided or reduced, and the liquid level of all the sub-chambers 140 can be at the same liquid level height.

In addition, in the prior art, a six-hole plate is used for coating, the six holes are all circular cylinders, the coating area is a square inscribed circle, the arc-shaped areas of the four sides are areas which cannot be reached by a coating head, and the waste area is large. Optionally, in the orifice plate described in this embodiment, when the orifice plate is used for coating, the sub-chamber 140 is shaped as a rectangular column, which has less wasted area and can effectively increase the available area of a single orifice. It will be understood by those skilled in the art that the shape of subchambers 140 other than rectangular columns does not preclude the use of other shapes such as cylindrical, hexagonal columns, etc.

In the prior art, eight-row and one-column structure orifice plates for coating are also adopted for coating; the channels of the eight-row one-column structure are identical at the ends, and the visual recognition during the course of picking the clone is affected due to the smaller size of the single row.

Referring to fig. 1-6, in an alternative of this embodiment, the partition 130 includes a first partition 131 and/or a second partition 132, i.e., the partition 130 includes the first partition 131 or the second partition 132, or the partition 130 includes the first partition 131 and the second partition 132.

The first partition 131 is cross-coupled to the second partition 132. That is, the chamber of the orifice plate is divided into a plurality of sub-chambers 140 by the first partition plate 131 and the second partition plate 132 which intersect with each other; compared with an eight-row one-column structure, the single-hole structure has the advantages that the size of a single hole can be effectively increased, and the single-hole structure is suitable for visual identification during the process of selecting the clone. Alternatively, the first partition 131 is perpendicular to the second partition 132; that is, the chamber of the orifice plate is divided into a plurality of sub-chambers 140 by the first partition plate 131 and the second partition plate 132 which are perpendicular to each other; compared with an eight-row one-column structure, the single-hole structure has the advantages that the size of a single hole can be effectively increased, and the single-hole structure is suitable for visual identification during the process of selecting the clone.

Optionally, the number of the first partition plates 131 is one or more; for example, the number of the first partitions 131 is 1, 3, 4, or other numbers.

Optionally, the number of the second partition plates 132 is one or more; for example, the number of the second partitions 132 is 1, 3, 4, or other numbers.

Optionally, the bottoms of the first partition plate 131 and the second partition plate 132 are provided with a plurality of partition plate through holes 133, so that the liquid levels of all the sub-chambers 140 are at the same liquid level, the experimental effect is guaranteed to a certain extent, and the working efficiency is improved.

In an alternative of this embodiment, the partition 130 includes only the first partitions 131, and the number of the first partitions 131 is three. The first partition 131 divides the chamber into four sub-chambers 140 so that the orifice plate is a four-orifice plate.

Referring to fig. 1 and 3, in an alternative of the present embodiment, the partition 130 includes first partitions 131 and second partitions 132, the number of the first partitions 131 is three, and the number of the second partitions 132 is one.

The first partition 131 is connected perpendicularly across the second partition 132 to divide the chamber into eight sub-chambers 140 so that the orifice plate is an eight-orifice plate. The eight-hole plate can effectively increase the size of a single hole and is suitable for visual identification during the course of selecting the clone. Alternatively, the sub-chamber 140 of the eight-hole plate is shaped as a rectangular column, which wastes less area and effectively increases the available area of a single hole.

Alternatively, three first partitions 131 are arranged in parallel at equal intervals between the side wall 110 and the base 120, and the second partitions 132 are perpendicularly cross-coupled on the central axis of the first partitions 131.

The first partition 131 includes at least one partition through-hole, and the second partition 132 includes at least one partition through-hole, so that all the sub-chambers communicate with each other.

Alternatively, the three first partitions 131 respectively include one partition through hole, and the second partition 132 includes one partition through hole.

The partition through holes of the second partition 132 are respectively connected to the partition through holes of the three first partitions 131 so as to intersect with each other, so that all the sub-chambers communicate with each other.

Alternatively, the longitudinal length of the partition through holes of the second partition 132 in the length direction of the second partition 132 is greater than the spacing distance of the three first partitions 131. That is, 4 chambers formed by the three first partitions 131 can be communicated through the partition through-holes of the second partition 132.

In this embodiment, the larger the through holes of the partition plate, the faster each sub-chamber 140 can reach the same height plane when pouring the culture solution.

In the alternatives of this embodiment, the number of the partition through-holes 133 of the first partition 131 is one or more; alternatively, the number of the partition through-holes 133 of the first partition 131 is one.

In the alternative of this embodiment, the number of the partition through-holes 133 of the second partition 132 is one or more; alternatively, the number of the partition through holes 133 of the second partition 132 is one.

In an alternative of this embodiment, the partition through hole 133 of the first partition 131 is abutted against or spaced from the bottom plate 120; optionally, the partition through hole 133 of the first partition 131 abuts the bottom plate 120.

In an alternative of the present embodiment, the partition through-holes 133 of the second partition 132 are abutted against or spaced from the bottom plate 120; optionally, the partition through hole 133 of the second partition 132 abuts the bottom plate 120.

The partition through holes 133 of the first partition plate 131 or the partition through holes 133 of the second partition plate 132 are spaced from the bottom plate 120, that is, the partition through holes 133 are not connected with the bottom plate 120 and have a certain distance, but the requirement that all the sub-chambers can be communicated when the culture solution is poured is met, and the liquid levels of all the sub-chambers are consistent.

In an alternative of this embodiment, all the partition through-holes 133 may be the same or different in number, or some of the partition through-holes 133 may be the same in number. The specific number of the partition through holes 133 may be determined according to actual needs.

Referring to fig. 1-6, in an alternative version of this embodiment, the well plate further includes a floor support wall 150;

the bottom plate supporting walls 150 are disposed around the bottom plate 120, and the bottom plate supporting walls 150 are connected to the bottom of the bottom plate 120; the base plate 120 is supported by the base plate support wall 150 so that the base plate 120 is disposed at a predetermined height.

Optionally, the side walls 110 are spaced from the edges of the base plate 120. By the spacing of the side walls 110 from the edges of the base plate 120 to facilitate capping.

The pore plate in this embodiment is a novel pore plate with a small waste area and can be used for coating, and the problems of inconsistent height of the culture liquid and easy cross contamination are effectively solved through the partition plate 130 and the partition plate through holes 133. For example, compared with a conventional six-well plate, the single-well usable area of the plate in this embodiment is three times larger than that of the six-well plate when the plate is used for coating, thereby effectively reducing the waste of culture area.

In this embodiment, when the pore plate is an eight-pore plate, the eight pores are communicated through the partition through hole 133, and when the culture medium is poured, the eight pores can be communicated with each other to ensure that the culture medium in each pore is consistent in height, and meanwhile, the poured culture medium only needs to be higher than the partition through hole 133, so that the pores can be ensured to be relatively blocked, and the diffusion and influence of the aerosol can be avoided.

This embodiment also provides a system comprising the well plate of any of the above embodiments, wherein the well plate is used for coating or culturing. Systems including an orifice plate, such as an automated coating system.

In the embodiment, the system comprising the orifice plate divides the chamber into a plurality of sub-chambers 140 by the partition plate 130 arranged on the orifice plate, so as to avoid or reduce the problem of aerosol mutual pollution existing in one orifice plate to a certain extent; the bottom of the partition plate 130 is provided with a plurality of partition plate through holes 133, and all the sub-chambers 140 are communicated through the partition plate through holes 133, so that the liquid levels of all the sub-chambers 140 are at the same liquid level, and the experimental effect is guaranteed to a certain extent.

The system including the orifice plate provided by the embodiment includes the orifice plate, and the technical features of the orifice plate disclosed in the above are also applicable to the system including the orifice plate, and the technical features of the orifice plate disclosed in the above are not described repeatedly. The system including the orifice plate in this embodiment has the advantages of the orifice plate described above, and the advantages of the orifice plate disclosed above will not be described again.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An orifice plate comprising a sidewall and a floor; the side walls and the floor form a chamber;

and a plurality of partition plate through holes are formed in the bottoms of the partition plates, so that all the sub-chambers are communicated with each other.

2. The orifice plate of claim 1, wherein the baffle through-hole extends to the floor.

3. The orifice plate of claim 2, wherein the shape of the baffle through-holes comprises any one of a circle, an ellipse, an arc, a polygon, or a combination thereof.

4. The orifice plate of claim 3, wherein the baffle through-holes are rectangular in shape.

5. The well plate according to any of claims 1-4, characterized in that the separator comprises a first separator and/or a second separator; the first partition plate is connected with the second partition plate in a crossing manner;

the number of the first partition plates is one or more;

the number of the second partition plates is one or more.

6. The orifice plate of claim 5, wherein the baffles comprise a first baffle and a second baffle, and the number of the first baffles is three and the number of the second baffles is one;

7. The well plate according to claim 6, wherein three of said first partition plates are arranged in parallel at equal intervals between said side wall and said bottom plate, and said second partition plates are perpendicularly cross-connected on a central axis of said first partition plates;

8. The orifice plate of claim 7, wherein each of the three first baffles includes a baffle through-hole, and the second baffle includes a baffle through-hole;

9. The orifice plate of claim 8, wherein the longitudinal length of the second baffle through-hole in the direction of the second baffle length is greater than the separation distance of three of the first baffles.

10. A system comprising a well plate according to any of claims 1 to 9, wherein the well plate is used for coating or culturing.