CN217856233U - Micro-fluidic system integrating DNA extraction and detection - Google Patents
Micro-fluidic system integrating DNA extraction and detection Download PDFInfo
- Publication number
- CN217856233U CN217856233U CN202221560321.3U CN202221560321U CN217856233U CN 217856233 U CN217856233 U CN 217856233U CN 202221560321 U CN202221560321 U CN 202221560321U CN 217856233 U CN217856233 U CN 217856233U
- Authority
- CN
- China
- Prior art keywords
- dna
- dna extraction
- reaction
- paraffin
- microfluidic system
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model provides a micro-fluidic system of integrated DNA extraction detection relates to molecular biology technical field and micro-fluidic chip technical field. The micro-fluidic chip comprises a chip main body, and a liquid storage pool, a DNA elution part, a channel, a paraffin storage part and a reaction pool which are arranged on the chip main body. By eluting DNA at one time, multiple reactions can be realized, and one DNA elution part can correspond to one or more reaction pools. In addition, the microfluidic system also releases DNA extraction work from a laboratory, can meet the requirement of on-site rapid detection, can be used for skillfully mastering the operation process through simple training for operators, and greatly reduces the operation difficulty of the operators. The paraffin storage part is arranged, so that the detection accuracy is improved.
Description
Technical Field
The utility model relates to a molecular biology technical field and micro-fluidic chip technical field particularly, relate to a micro-fluidic system that integrated DNA draws detection.
Background
At present, PCR method is mostly used for detecting nucleic acid, and the method needs to use precise equipment for nucleic acid amplification and result display. And the method has relatively high technical requirements and is difficult to meet the rapid detection requirements of the site. Taking a nucleic acid detection method as an example, an antigen-antibody detection method is adopted, is mainly used for sample detection in an acute infection stage, namely within 7 days of symptoms of suspected people, and has certain limitation. In contrast, PCR technology based on DNA molecular diagnosis has the characteristics of early diagnosis, high sensitivity and high specificity. However, the PCR technology still has the problems of incapability of rapid detection and high detection cost. If the molecular detection can be carried out in a laboratory, applied to emergency treatment, followed by detection, even applied to daily household, the application of molecular diagnosis can be really and greatly promoted. In addition, one of the next breakthrough directions in molecular diagnostics is the development of certain high-throughput molecular detection reagents and extraction techniques.
The microfluidic chip technology integrates units such as sample pretreatment, purification, detection and the like in the analysis process in the fields of biology, medicine and the like on a micron-scale microfluidic chip by accurately controlling fluid through a microchannel and by means of a reaction chamber, a component and the like, has the advantages of controllable liquid flow, integration, low consumption, high flux, quick analysis and the like, and is widely applied to the research fields of biomedicine, environmental science and the like.
In view of this, the utility model is particularly proposed to realize the rapid extraction and accurate detection of DNA.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide an integrated DNA draws micro-fluidic system who detects in order to solve above-mentioned technical problem.
The utility model discloses a realize like this:
the utility model provides an integrated DNA draws micro-fluidic system that detects, it includes micro-fluidic chip, micro-fluidic chip includes the chip main part, and the liquid storage tank that sets up in the chip main part, DNA elution portion, the passageway, paraffin storage portion and reaction tank, liquid storage tank and DNA elution portion intercommunication, DNA elution portion passes through passageway and reaction tank intercommunication, it sets up two paraffin storage portions to correspond on the passageway, and one of them paraffin storage portion is close to the reaction tank setting, another paraffin storage portion is kept away from the reaction tank setting and paraffin storage portion and is through setting up paraffin flowing groove and passageway intercommunication.
The inventor provides a microfluidic system, and a liquid reservoir is arranged on a chip body to satisfy the storage including but not limited to TE buffer and Mix reagent. The DNA elution part is used for eluting a sample to be detected, so that the DNA of the sample to be detected is dissociated under the liquid elution effect of the liquid storage tank, and the subsequent reaction in the reaction tank is facilitated. Two paraffin storage parts are arranged to meet the functions of intercepting liquid and sealing reaction liquid. Specifically, the paraffin storage part arranged close to the reaction tank is arranged on the channel, and before the paraffin storage part is not heated, the paraffin in the paraffin storage part is in a solid state at the temperature lower than the melting point, so that the channel can be blocked, the buffer solution or Mix with the sample DNA in the channel cannot flow downwards into the reaction tank, and the interception effect is achieved. After interception, by swinging the chip, equal volume of DNA + Mix mixed solution can be uniformly distributed in the channel of each chip. In addition, the reaction liquid can be ensured not to contact with the freeze-dried primer in the reaction tank before being heated.
After heating, the paraffin close to the paraffin storage part of the reaction tank is melted, and under the action of gravity or through throwing the chip, the DNA + Mix mixed solution flows into the reaction tank of the chip for reaction, and a paraffin film is formed on the reaction solution after the paraffin storage part arranged in the other raw material reaction tank is melted, so that the evaporation of the reaction solution and the cross contamination of aerosol can be prevented.
The microfluidic system can realize multiple reactions by eluting DNA at one time, and one DNA eluting part can correspond to one or more reaction cells. In addition, the microfluidic system also releases DNA extraction work from a laboratory, can meet the requirement of on-site rapid detection, can be used for skillfully mastering the operation process through simple training for operators, and greatly reduces the operation difficulty of the operators.
In a preferred embodiment of the present invention, the number of the channels is at least two. For example 2-50, or 2-25, or 2-10. The utility model provides a channel figure that sets up among the micro-fluidic system can adjust according to the demand of actual detection target gene and the detection demand of repeatability experiment.
The multiple channels are arranged, so that DNA can be eluted at one time, and multiple reactions can be realized. And the on-site rapid detection is facilitated.
In the preferred embodiment of the present invention, the number of the reaction cells is the same as the number of the channels, and the reaction cells are embedded with primers for detecting DNA. Including, but not limited to, LAMP primers, PCR primers, and the like.
In the preferred embodiment of the present invention, a finger pressing film is further disposed on the top of the liquid storage tank. The pressure-indicating membrane is arranged so that the elution solution or buffer solution in the reservoir is pressed out to the DNA elution part by pressing the pressure-indicating membrane under the difference between the internal pressure and the external pressure.
In a preferred embodiment of the present invention, the microfluidic system further comprises a DNA extraction device, wherein the DNA extraction device comprises a substrate and a needle disposed on the substrate.
In an alternative embodiment, the extraction of nucleic acids is performed by piercing the biological tissue sample with a tungsten needle or a hard material needle with a surface modified nucleic acid binding group.
A tungsten needle is a structurally elongated tungsten article made of pure tungsten or a tungsten alloy. High hardness and sharp needle point. The tungsten needle can be used for extracting the nucleic acid of the biological tissue sample. Utilize the tungsten needle to draw sample DNA, liberating DNA extraction work from the laboratory in very big degree, can realizing on-the-spot short-term test, to operating personnel, can be skilled master operation procedure through simple training.
Nucleic acid binding groups include, but are not limited to, one or more of hydroxyl, silica, chitosan, poly diallyldimethylammonium chloride.
In an alternative embodiment, the method for surface modification of nucleic acid binding groups comprises the steps of coating an aluminum film on the surface of a tungsten needle by using a sodium hydroxide solution, cleaning and storing for later use.
In an alternative embodiment, the method for surface modification of the nucleic acid binding group comprises plating polyvinyl alcohol on the surface of the polydimethylsiloxane needle, then modifying poly diallyl dimethyl ammonium chloride, and washing and storing for later use.
The above cleaning includes ultrasonic cleaning by completely infiltrating the tungsten needle or the polydimethylsiloxane needle with an SDS solution. In an alternative embodiment, the tungsten needle or the polydimethylsiloxane needle is completely infiltrated with 0.3 to 0.6mol/L SDS solution for 8 to 12min of ultrasonic cleaning. After ultrasonic cleaning, the mixture is cleaned by ultrapure water until no foam appears.
In the preferred embodiment of the present invention, the tungsten needle is detachably connected to the substrate. The arrangement is convenient for cleaning the tungsten needle and preventing sample pollution.
In a preferred embodiment of the present invention, the number of the tungsten needles is plural.
In a preferred embodiment of the present invention, the number of the tungsten needles is 1-19.
In a preferred embodiment of the present invention, the substrate has a circular, elliptical or polygonal shape.
In the preferred embodiment of the present invention, the two adjacent channels are not crossed. And the channel is a transparent visual channel. And the distance between the paraffin storage part of each channel close to the reaction tank and the reaction tank is the same. The intervals are the same, so that when the paraffin storage part close to the reaction tank plays a role in interception, equal volume of DNA + Mix mixed liquor is uniformly distributed in each channel.
Compared with the prior art, the beneficial effects of the utility model are that:
the utility model provides a micro-fluidic system can realize multiple reaction through disposable elution DNA, and a DNA elution portion can correspond one to a plurality of reaction tanks. In addition, the microfluidic system also frees DNA extraction work from a laboratory, can meet the requirement of on-site rapid detection, can skillfully master the operation process through simple training for operators, and greatly reduces the operation difficulty of the operators.
The paraffin storage part can prevent the reaction liquid from evaporating and the cross contamination of aerosol on one hand, the second aspect can also make the amount of the reaction liquid in the channel consistent, and the third aspect can avoid the contact with the primer in the reaction pool before the thermal reaction. Based on the above setting, the accuracy of detection is favorably improved.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 is a front view of a microfluidic chip structure;
FIG. 2 is a three-dimensional schematic view of a microfluidic chip;
FIG. 3 is a front view of a tungsten needle structure;
FIG. 4 is a three-dimensional schematic view of a pin header;
FIG. 5 shows the chip reaction tank after shaking;
FIG. 6 is a statistical chart of the reaction results of the RT-LAMP reaction of the DNA extracted by the tungsten needle and the DNA extracted by the kit;
FIG. 7 is a diagram showing the amplification result of sheep meat;
FIG. 8 is a graph showing the results of amplification of pork.
Icon: 1-a finger-pressure membrane liquid storage pool; 2-DNA elution part; 3-sealing the paraffin groove; 4-a cut-off paraffin tank; 5-a reaction tank; 6-microfluidic chip body.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of the embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined or explained in subsequent figures.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", "clockwise", "counterclockwise" and the like indicate the position or positional relationship based on the position or positional relationship shown in the drawings, or the position or positional relationship which is usually placed when the product of the present invention is used, and the terms are only used for convenience of description of the present invention and simplification of description, but do not indicate or imply that the device or element to which the term refers must have a specific position, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.
Furthermore, the terms "horizontal", "vertical", "overhang" and the like do not imply that the components are required to be absolutely horizontal or overhang, but may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.
In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood as a specific case by those skilled in the art.
Example 1
Please refer to fig. 1 and fig. 2, the utility model provides a micro-fluidic system for integrated DNA extraction and detection, which comprises a micro-fluidic chip, the micro-fluidic chip comprises a micro-fluidic chip main body 6, and a finger-pressing membrane liquid storage tank 1, a DNA elution part 2, channels (not shown in the figure, namely, a plurality of micro-channels which are transversely arranged as shown in fig. 1), a paraffin storage part and a reaction tank 5, the finger-pressing membrane liquid storage tank 1 is communicated with the DNA elution part 2, the DNA elution part 2 is communicated with the reaction tank 5 through the channels, and two paraffin storage parts are correspondingly arranged on one channel.
The material of the chip body is PMMA (polymethyl methacrylate), which means polymethyl methacrylate, and is processed by a CNC (numerical control machine).
In this embodiment, the paraffin wax reservoir is in the form of a paraffin wax groove. Specifically, paraffin storage portion is including closing paraffin groove 3 and damming paraffin groove 4, and damming paraffin groove 4 is close to reaction tank 5 and sets up, and closed paraffin groove 3 keeps away from reaction tank 5 and sets up, and just flows groove and passageway intercommunication through setting up paraffin.
The melting point of paraffin is 45 ℃, and the reaction temperature of the reaction tank 5 is 65 ℃. In the reaction process, after the quantitative Mix solution for cleaning the tungsten needle elutes the DNA on the tungsten needle system, the DNA flows into each channel. Due to the interception of the cut-off paraffin tank 4, equal volume of DNA + Mix mixture was distributed in each channel, and the volume was about 20. Mu.L. The intercepting paraffin tank 4 can make the reaction liquid amount in each channel consistent on one hand, and can also ensure that the reaction liquid does not contact with the freeze-dried primer in the reaction tank 5 before heating on the other hand.
After heating at 65 ℃ for about 5min, paraffin melts, the chip is swung to enable the mixed solution to flow into the reaction tank 5 (shown in figure 5), and a layer of paraffin film is formed on the reaction solution after the paraffin in the closed paraffin tank 3 melts, so that the reaction solution evaporation and the cross contamination of aerosol are prevented, and the detection accuracy is improved.
The number of channels in this embodiment is 12. The number of the reaction pools 5 is the same as that of the channels, and LAMP primers for DNA detection are embedded in the reaction pools 5. Before extraction and LAMP reaction, 4.4. Mu.l of primers are pre-embedded in the reaction tank 5 and dried.
The microfluidic system also comprises a DNA extraction device, wherein the DNA extraction device comprises a substrate and a tungsten needle arranged on the substrate.
In this embodiment, a circular acrylic plate is used for punching (see fig. 3 and 4), and 1-19 tungsten needles (22 in this embodiment) for DNA extraction can be installed according to the need of extracting a sample, which is convenient for an operator to extract DNA. The radius of the circular acrylic plate is 8mm, and the radius of the tungsten needle is 0.75mm.
In an alternative embodiment, a cuboid acrylic plate is used as a substrate, and 1-19 DNA extraction tungsten needles can be installed according to the requirement of extracting a sample, so that an operator can conveniently extract DNA. In this embodiment, the tungsten needle is not specially modified, and only the physical adsorption of the surface of the tungsten needle to DNA is relied on.
The detection step of DNA extraction and amplification by using a microfluidic system is as follows:
(1) And (3) extracting DNA.
Randomly selecting a row of tungsten needles to manufacture a DNA extraction device, lightly pressing the tungsten needles on the surface of a sample to be detected, and quickly transferring the DNA extraction device to a DNA elution channel on a microfluidic chip after staying for 1 min; after the DNA extraction device is placed, the top end of the microfluidic chip is pressed to press a pressure indicating membrane, TE eluent and LAMP Mix mixed liquid in the liquid storage tank flow out, the liquid completely flows through the tungsten needle and reaches the DNA elution part 2, the DNA extraction device is taken out, and the microfluidic chip is swung to enable mixed reaction liquid to enter a channel.
(2) Amplification of DNA:
vertically inserting one end of the microfluidic chip reaction tank 5 into the microfluidic chip reaction generating device, pressing a Strat button to start a reaction program, waiting for 30-45 minutes (if paraffin of the cut-off paraffin tank 4 melts after the reaction starts for 5 minutes and the reaction liquid does not enter the reaction tank 5, shaking the microfluidic chip again to enable the mixed reaction liquid to enter the reaction tank 5), and ending the reaction until the reaction tank 5 in the reaction tank 5 is completely yellowed.
(3) And (4) judging a result:
observing the color of the reaction liquid in the standard reaction tank 5, comparing the color with a standard color comparison card, judging that the red result is negative and does not contain the species corresponding to the primer; the yellow result is judged to be positive, namely the yellow result contains the species corresponding to the primer.
Experimental example 1
In this experimental example, the DNA extraction device and the tiangen kit provided in example 1 were used to extract sheep meat DNA for RT-LAMP amplification detection, respectively, and the DNA extraction effect was verified.
The reaction system is shown in the following table:
| 2x RT- |
10 |
| mixed primer | 4.4 |
| |
4 |
| ddH 2 O | 1.2 |
| SYBR fluorescent dyes | 0.4 |
| Total | 20(μL) |
The effect of the DNA extraction device is verified by adopting different numbers of tungsten needles to manufacture the DNA extraction device. Specifically, 1, 2, 4, 6 and 8 tungsten needles are respectively used for preparing a DNA extraction device, DNA extraction is respectively carried out on sheep mutton, and then 100 mu L ddH is used 2 And (4) eluting the DNA, comparing the eluted DNA with the DNA obtained by extracting sheep meat by using a Tiangen kit, and carrying out RT-LAMP (reverse transcription loop-mediated isothermal amplification) experiment to obtain an amplification result. The results are shown in FIG. 6.
As can be seen from FIG. 6, the concentration of DNA extracted by the kit was the highest, and the Ct value of the amplification curve was 17min. Ct of DNA extracted by the tungsten needle is as follows: 8. the number of 6, 4, 2 and 1 needles is 26, 28, 32, 38 and 39min respectively; negative control water had no curve. The concentration and purity of the DNA extracted by the tungsten needle can meet the amplification requirement.
Experimental example 2
Sheep meat DNA was extracted using the microfluidic system provided in example 1 and the results were examined according to the detection method provided in example 1. After the meat sample is pricked by a tungsten needle, the meat sample is put into a chip and heated for 60 minutes at 65 ℃, and the color change is observed.
The reaction system is as follows:
| |
10 |
| mixed primer | 4.4 |
| |
4 |
| ddH 2 O | 1.6 |
| Total | 20(μL) |
Mix is the WarmStart Colorimetric LAMP 2X Master Mix (New England Biolabs).
FIG. 7 shows the result of DNA amplification of sheep meat extracted by the DNA extraction apparatus. The universal primers, blank (without primers), chicken, pig, sheep and mouse primers are respectively pre-lyophilized and embedded in the No. 1-6 holes on the left side (from left to right) of the chip. The right side of the chip corresponds to the chip, and universal primers, blank (without primers), chicken, pig, sheep and mouse primers are respectively pre-lyophilized and embedded in the No. 1-6 holes from the right to the left. The result shows that the microfluidic system integrating the DNA extraction detection can specifically realize the detection of the target sample.
The sequences of the primers used are shown in the table below.
The meat primer sequences are as follows:
experimental example 3
The microfluidic system provided in example 1 was used to extract mouse meat DNA and the results were tested according to the test method provided in example 1. After the meat sample is pricked by the tungsten needle, the meat sample is put into a chip and heated for 60 minutes at 65 ℃, and the color change is observed.
The primer sequences are shown in reference experiment example 2, and the reaction system is as follows:
| |
10 |
| mixed primer | 4.4 |
| |
4 |
| ddH 2 O | 1.6 |
| Total | 20(μL) |
FIG. 8 shows the amplification result of the DNA extracted from sheep meat by the DNA extraction apparatus. The universal primers, blank (without primers), chicken, pig, sheep and mouse primers are respectively pre-lyophilized and embedded in the No. 1-6 holes on the left side (from left to right) of the chip. Corresponding to the right side of the chip, from right to left, no. 1-6 wells are respectively pre-lyophilized and embedded with universal primers, blank (without primers), chicken, pig, sheep, and mouse primers. The result shows that the microfluidic system integrating the DNA extraction detection can specifically realize the detection of the target sample.
To sum up, the utility model provides a micro-fluidic system can realize multiple reaction through disposable elution DNA, and a DNA elution portion 2 can correspond one to a plurality of reaction tank 5. In addition, the microfluidic system also frees DNA extraction work from a laboratory, can meet the requirement of on-site rapid detection, can skillfully master the operation process through simple training for operators, and greatly reduces the operation difficulty of the operators. The detection accuracy of the microfluidic system is high.
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The microfluidic system is characterized by comprising a microfluidic chip, wherein the microfluidic chip comprises a chip main body, and a liquid storage tank, a DNA elution part, channels, a paraffin storage part and a reaction tank which are arranged on the chip main body, the liquid storage tank is communicated with the DNA elution part, the DNA elution part is communicated with the reaction tank through the channels, one channel is correspondingly provided with two paraffin storage parts, one paraffin storage part is arranged close to the reaction tank, the other paraffin storage part is arranged far away from the reaction tank, and the paraffin storage part is communicated with the channels through a paraffin flowing groove.
2. The microfluidic system for integrated DNA extraction detection according to claim 1, wherein the number of channels is at least two.
3. The microfluidic system for integrated DNA extraction detection according to claim 2, wherein the number of the reaction cells is the same as the number of the channels, and the reaction cells are embedded with primers for DNA detection.
4. The microfluidic system for integrated DNA extraction and detection according to claim 1, wherein a pressure-indicating membrane is further disposed on the top of the reservoir.
5. The microfluidic system for integrated DNA extraction detection according to claim 1, further comprising a DNA extraction device comprising a substrate and a needle disposed on the substrate.
6. The microfluidic system for integrated DNA extraction detection according to claim 5, wherein the needle is a tungsten needle, and the tungsten needle is detachably connected to the substrate.
7. The microfluidic system for integrated DNA extraction detection according to claim 6, wherein the number of tungsten needles is plural.
8. The microfluidic system for integrated DNA extraction detection according to claim 6, wherein the number of tungsten needles is 1-19.
9. The microfluidic system for integrated DNA extraction detection according to claim 6, wherein the substrate has a circular, elliptical or polygonal shape.
10. The microfluidic system for integrated DNA extraction and detection according to claim 1, wherein two adjacent channels do not intersect with each other, and each channel has the same distance from the paraffin storage part near the reaction cell.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221560321.3U CN217856233U (en) | 2022-06-21 | 2022-06-21 | Micro-fluidic system integrating DNA extraction and detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221560321.3U CN217856233U (en) | 2022-06-21 | 2022-06-21 | Micro-fluidic system integrating DNA extraction and detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217856233U true CN217856233U (en) | 2022-11-22 |
Family
ID=84098024
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221560321.3U Active CN217856233U (en) | 2022-06-21 | 2022-06-21 | Micro-fluidic system integrating DNA extraction and detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217856233U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114150042A (en) * | 2022-01-07 | 2022-03-08 | 中国农业科学院农业质量标准与检测技术研究所 | Method for integrating DNA extraction and LAMP visual nucleic acid detection |
-
2022
- 2022-06-21 CN CN202221560321.3U patent/CN217856233U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114150042A (en) * | 2022-01-07 | 2022-03-08 | 中国农业科学院农业质量标准与检测技术研究所 | Method for integrating DNA extraction and LAMP visual nucleic acid detection |
| CN114150042B (en) * | 2022-01-07 | 2024-03-15 | 中国农业科学院农业质量标准与检测技术研究所 | Method for integrating DNA extraction and LAMP visual nucleic acid detection |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US6703247B1 (en) | Apparatus and methods for efficient processing of biological samples on slides | |
| CN101990516B (en) | Multiplex sample preparation system and the use in integrated analysis system thereof | |
| CN105296349A (en) | Microfluidic chip, detection system and device used for rapid DNA detection | |
| US8828663B2 (en) | Thermal reaction device and method for using the same | |
| EP2430177B1 (en) | Apparatus for performing amplicon rescue multiplex pcr | |
| EP2518161A1 (en) | Method for detection of mutant alleles | |
| CN217856233U (en) | Micro-fluidic system integrating DNA extraction and detection | |
| US20090061450A1 (en) | System and method for diagnosis of infectious diseases | |
| US20090325278A1 (en) | Reaction System for Performing in the Amplification of Nucleic Acids | |
| TW200406487A (en) | Microfluidic system for analyzing nucleic acids | |
| CN101928663B (en) | Integrated fluidic chip device for digital nucleic acid amplification and application | |
| CN205170857U (en) | A micro -fluidic chip , detecting system and device for DNA short -term test | |
| CN105170206A (en) | Micro-fluidic chip achieving multi-index detection | |
| EP1463796A1 (en) | Microfluidic device and methods of using same | |
| EP1563907A2 (en) | Biochemical reaction cartridge | |
| DE102005052752A1 (en) | Apparatus and method for detecting molecular interactions | |
| US20150209789A1 (en) | Sample processing apparatus and automatic analyzing apparatus including the same | |
| WO2007106552A2 (en) | System and method for diagnosis of infectious diseases | |
| KR102041217B1 (en) | Multi-channel device for downwardly injecting liquid sample, device for extracting nucleic acid comprising the same, and method for extracting nucleic acid using the same | |
| CN105296348A (en) | Genotyping detection-based microfluidic chip, detection system and device | |
| CN113512490A (en) | Self-driven micro-fluidic detection device and application thereof | |
| JP2010538672A (en) | Method, apparatus and molecular biology kit for extracting amplified genetic material | |
| US20020106787A1 (en) | Device for repid DNA sample processing with integrated liquid handling, thermocycling, and purification | |
| CN216998418U (en) | Micro-fluidic chip integrating DNA extraction and nucleic acid visual detection and micro-fluidic system | |
| EP4286509A1 (en) | Nucleic acid detection apparatus and nucleic acid detection method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |