CN217757478U - Micro-fluidic chip - Google Patents

Abstract

The utility model relates to a micro-fluidic chip, which comprises a chip body, wherein a plurality of detection units are arranged on the chip body, and each detection unit comprises a sample adding port, a liquid drop generation space, a liquid drop tiling space, an oil discharge space, a waste liquid storage space and an air outlet which are sequentially communicated; the sample adding port is used for adding a sample, the liquid drop generating space is used for converting the sample into liquid drops and flatly paving the liquid drops in the liquid drop paving space, the oil discharging space is used for guiding waste liquid to the waste liquid storage space, and the exhaust port is used for exhausting air during sample adding; the sample adding ports of all the detection units are positioned on a first virtual circle, the exhaust ports of all the detection units are positioned on a second virtual circle, and the first virtual circle and the second virtual circle are mutually nested. The micro-fluidic chip optimizes the layout of the detection units on the chip body, reasonably utilizes the space on the chip body, and can further miniaturize the micro-fluidic chip under the condition of distributing the same number of detection units.

Description

Micro-fluidic chip

Technical Field

The utility model relates to a biological detection technical field especially relates to a micro-fluidic chip.

Background

The Polymerase Chain Reaction (PCR) technology is one of the most important tools in modern biology, and is widely applied to aspects such as medical diagnosis, individualized medicine, food detection, transgenic biological detection, pathogen identification, immunoassay, forensic science and the like. As the latest generation of PCR technology, digital PCR technology developed and produced based on microfluidic technology is widely used due to its smaller reaction volume, faster reaction speed, lower system noise and higher sensitivity than the conventional PCR technology.

The rapid development of the microfluidic chip technology enables the digital PCR technology to rapidly and accurately decompose the sample fluid into nanoliters or even pico-upgrades, so that more sample dispersion numbers are obtained, and multi-step parallel reaction is carried out, thereby further improving the detection sensitivity of the digital PCR technology.

The currently used droplet preparation methods include a T-junction (T-junction) and a Flow-focusing (Flow-focusing). The system of the whole equipment of the methods is complex, the requirement on a fluid control system is high, and the number of effective liquid drops obtained finally is relatively small. And the traditional micro-fluidic chip is unreasonable in layout, so that the space of the chip is greatly wasted, and the micro-fluidic chip is limited to be further miniaturized under the condition that the same number of detection units are distributed.

SUMMERY OF THE UTILITY MODEL

In view of the above, there is a need to provide a microfluidic chip capable of improving the above problem, in order to solve the problem that the conventional microfluidic chip is not reasonable in layout and further miniaturization thereof is limited.

A micro-fluidic chip comprises a chip body, wherein a plurality of detection units are arranged on the chip body, and each detection unit comprises a sample adding port, a droplet generation space, a droplet tiling space, an oil discharge space, a waste liquid storage space and an exhaust port which are sequentially communicated; the sample adding port is used for adding a sample, the liquid drop generating space is used for converting the sample into liquid drops and flatly paving the liquid drops in the liquid drop paving space, the oil discharging space is used for guiding waste liquid to the waste liquid storage space, and the air exhaust port is used for exhausting air during sample adding;

the sample loading ports of all the detection units are positioned on a first virtual circle, the exhaust ports of all the detection units are positioned on a second virtual circle, and the first virtual circle and the second virtual circle are nested with each other.

According to the microfluidic chip, the sample adding ports of all the detection units are positioned on the first virtual circle, the exhaust ports of all the detection units are positioned on the second virtual circle, and the first virtual circle and the second virtual circle are mutually nested. Therefore, the layout of the detection units on the chip body is optimized, the space on the chip body is reasonably utilized, and the micro-fluidic chip can be further miniaturized under the condition of distributing the same number of detection units. Meanwhile, the arrangement enables the structure of the detection instrument used in cooperation with the micro-control flow chip to be simple.

In one embodiment, at least two of the detection units share one of the exhaust ports.

In one embodiment, the microfluidic chip comprises a plurality of groups of detection units sequentially arranged at intervals in the circumferential direction of the chip body, and each group of detection units comprises at least two detection units;

all the detection units included in each group of detection unit group share one air outlet.

In one embodiment, all the detection units included in each group of detection unit groups intersect with the exhaust port from two circumferential sides of the chip body.

In one embodiment, the chip body is a disk shape, and the first virtual circle and the second virtual circle are both concentric with the chip body.

In one embodiment, the droplet generation space comprises a plurality of droplet generation channels communicated between the sample addition port and the droplet tiling space, and all the droplet generation channels have the same cross-sectional shape; the size of the liquid drop generating channel in the thickness direction of the chip body is smaller than that of the liquid drop tiling space;

each droplet generation channel comprises a first channel and a second channel which are communicated with each other, the first channel is communicated with the sample adding port, and the second channel is communicated with the droplet tiling space;

the second passage has a sectional area gradually increasing from one end communicating with the first passage to the other end.

In one embodiment, the first channel comprises a first sub-channel and a second sub-channel, the first sub-channel is communicated with the sample addition port, and the second sub-channel is communicated with the first sub-channel and the second channel;

the cross-sectional area of one end of the first sub-channel communicated with the second sub-channel is gradually increased to the other end of the first sub-channel.

In one embodiment, the oil discharge space includes a plurality of oil discharge passages, all of which have the same cross-sectional shape;

the size of the oil discharge channel in the thickness direction of the chip body is smaller than that of the liquid drop tiling space.

In one embodiment, the microfluidic chip further comprises a filter element, the filter element is arranged in each air outlet, and the filter elements are closed when meeting a liquid.

In one embodiment, the microfluidic chip further comprises a sealing film configured to be capable of being placed on the chip body to seal the sample addition port.

Drawings

Fig. 1 is a structural diagram of a microfluidic chip according to an embodiment of the present invention;

FIG. 2 is an exploded view of the microfluidic chip shown in FIG. 1;

FIG. 3 is a block diagram of a second sheet of the microfluidic chip shown in FIG. 2;

FIG. 4 is an enlarged view at A of the structure shown in FIG. 3;

fig. 5 is a partial structural view of the microfluidic chip shown in fig. 1.

100. A microfluidic chip; 10. a chip body; 11. a first sheet body; 12. a second sheet body; 13. a through hole; 20. a detection unit; 21. a sample addition port; 22. a microchannel; 23. a droplet generation space; 231. a droplet generation channel; 232. a first channel; 233. a first sub-channel; 234. a second sub-channel; 235. a second channel; 24. a droplet tiling space; 25. an oil discharge space; 251. an oil discharge channel; 26. a waste liquid storage space; 27. an exhaust port; 30. a filter element; 40. and (4) sealing films.

Detailed Description

In order to make the above objects, features and advantages of the present invention more comprehensible, embodiments of the present invention are described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, as those skilled in the art will be able to make similar modifications without departing from the spirit and scope of the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like, indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be interconnected within two elements or in a relationship where two elements interact with each other unless otherwise specifically limited. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In the present application, unless expressly stated or limited otherwise, the first feature may be directly on or directly under the second feature or indirectly via intermediate members. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.

Referring to fig. 1 and 2, an embodiment of the present invention provides a microfluidic chip 100, which includes a chip body 10, wherein a plurality of detecting units 20 are disposed on the chip body 10, so as to detect a plurality of samples simultaneously. At the time of detection, the sample is added to the detection unit 20, and finally collected and analyzed by optical data. It should be noted that the sample may be an extracted nucleic acid solution, and when the sample is added, the sample and the PCR reagent are mixed uniformly and then added to the detection unit 20.

Referring to fig. 2 and 3, each of the detecting units 20 includes a sample inlet 21, a droplet generating space 23, a droplet spreading space 24, an oil discharging space 25, a waste liquid storage space 26, and an air outlet 27, which are sequentially connected. The sample inlet 21 is used for adding a sample, the droplet generating space 23 is used for converting the sample into droplets to be tiled in the droplet tiling space 24, the oil discharging space 25 is used for guiding waste liquid to the waste liquid storage space 26, and the exhaust port 27 is used for exhausting air during sample addition. Specifically, the sample addition port 21 communicates with the droplet generation space 23 through the microchannel 22.

A continuous phase (droplet forming oil) is pre-packaged in each detection cell 20 and spreads throughout the droplet storage area. Of course, the continuous phase may not be pre-packaged in the detection unit 20, but may be added before the sample is added to the detection unit 20.

In the microfluidic chip 100, the sample is loaded into the detection unit 20 through the loading port 21, and a certain positive pressure is applied from the loading port 21 or a certain negative pressure is pumped from the exhaust port 27, so that the sample flows to the droplet generation space 23 under the driving of the pressure. When the sample flows through the droplet generation space 23, the sample turns into minute droplets and spreads in the droplet spreading space 24 until the droplets are uniformly spread over the entire droplet spreading space 24. Under the above-mentioned pressure, the excess droplet forming oil is discharged into the waste liquid storage region through the oil discharge space 25. The sample is subjected to a PCR reaction in the droplet plating space 24, and finally optical data collection and analysis are performed.

In one embodiment, the chip body 10 is a disc to facilitate processing of the chip body 10. The chip body 10 is provided with a through hole 13 in the middle for facilitating clamping.

In other embodiments, the shape of the chip body 10 is not limited, for example, the chip body 10 may also be a rectangular parallelepiped plate-shaped structure.

Referring to fig. 2, the chip body 10 includes a first sheet 11 and a second sheet 12 that are separately disposed, and the first sheet 11 and the second sheet 12 cover in a thickness direction and jointly define the plurality of detecting units 20. The thickness direction is also the axial direction of the chip body 10, and is also the Z direction in fig. 1. The chip body 10 is provided as two separate parts so as to facilitate opening of each detection unit 20.

Further, the sample addition port 21 and the exhaust port 27 are both opened in the first sheet member 11. The microchannel 22, the droplet generating space 23, the droplet tiling space 24, the oil discharging space 25, and the waste liquid storage space 26 are defined by the first sheet 11 and the second sheet 12. Specifically, the second sheet 12 is provided with a channel groove, and the first sheet 11 covers the second sheet 12 to close the channel groove, so as to form the microchannel 22, the droplet generation space 23, the droplet tiling space 24, the oil discharge space 25, and the waste liquid storage space 26 with the channel wall of the channel groove.

It should be understood that, in other embodiments, channel grooves may be formed on both the first sheet 11 and the second sheet 12, and the channel walls of the channel grooves on the first sheet and the second sheet define the micro channel 22, the droplet generating space 23, the droplet tiling space 24, the oil discharging space 25, and the waste liquid storage space 26.

In one embodiment, referring to fig. 5, the sample inlets 21 of all the detecting units 20 are located on a first virtual circle, the exhaust outlets 27 of all the detecting units 20 are located on a second virtual circle, and the first virtual circle and the second virtual circle are nested with each other. Thus, the layout of the detecting units 20 on the chip body 10 is optimized, the space on the chip body 10 is reasonably utilized, and the micro-fluidic chip 100 can be further miniaturized under the condition that the same number of detecting units 20 are distributed.

Further, when the chip body 10 is disc-shaped, the first virtual circle and the second virtual circle are concentric with the chip body 10, so as to further optimize the structure of the chip body 10, and achieve the purpose of further miniaturizing the microfluidic chip 100.

In one embodiment, the first virtual circle is nested within the second virtual circle. In another embodiment, the second virtual circle is nested within the first virtual circle. The two arrangements can achieve the purpose of miniaturizing the microfluidic chip 100.

Further, the diameter of the chip body 10 is set to 150 mm. It is contemplated that in other embodiments, the chip body 10 may take other shapes and is not limited to a specific size.

In one embodiment, at least two of the inspection units 20 share the exhaust port 27. Thus, the arrangement of the detection units 20 on the chip body 10 is optimized, and the microfluidic chip 100 can be further miniaturized with respect to the case where one exhaust port 27 is independently provided for each detection unit 20.

Referring to fig. 3, the microfluidic chip 100 includes a plurality of sets of detecting units sequentially arranged at intervals in a circumferential direction of the chip body 10 (see fig. 1, the circumferential direction is a surrounding direction of the exchanging Z direction), and each set of detecting units includes at least two detecting units 20. All the detecting units 20 included in each group of detecting unit group share one air outlet 27. Specifically, the microfluidic chip 100 includes four sets of detection units sequentially arranged at intervals in the circumferential direction of the chip body 10, each detection unit set includes four detection units 20, and the four detection units 20 in each set of detection unit set share one air outlet 27. In this way, the microfluidic chip 100 is capable of detecting 16 samples at a time.

It is contemplated that in other embodiments, the number of detection cell groups included in the microfluidic chip 100 is not limited, and the number of detection cells 20 included in each detection cell group is not limited. If the microfluidic chip 100 comprises two sets of detecting units, each set comprises 3 detecting units 20, etc.

In one embodiment, all the detecting units 20 included in each group of detecting units meet the air outlet 27 from two sides of the chip body 10 in the circumferential direction. Therefore, the mutual crossing between the detection units 20 included in each group of detection unit groups is avoided, the detection units 20 are convenient to arrange, and the spatial layout is more reasonable.

The microchannel 22 is arranged to extend between the sample port 21 and the droplet-generating space 23 in a meandering manner so as to guide the sample from the sample port 21 to the droplet-generating space 23. In other embodiments, microchannel 22 can extend in a straight line between sample port 21 and droplet generation space 23.

Referring to fig. 4, the droplet generation space 23 includes a plurality of droplet generation channels 231 communicating between the sample addition port 21 and the droplet tiling space 24, and all of the droplet generation channels 231 have the same cross-sectional shape. The size of the droplet generation channel 231 in the thickness direction of the chip body 10 is smaller than the size of the droplet tiling space 24. Each droplet generation channel 231 includes a first channel 232 and a second channel 235 that communicate with each other, the first channel 232 communicating with the sample addition port 21, and the second channel 235 communicating with the droplet tiling space 24. The second passage 235 has a sectional area gradually increasing from one end communicating with the first passage 232 to the other end, so that the second passage 235 has a flared trumpet shape.

In the above arrangement, the droplet generation channel 231 and the droplet tiling space 24 are arranged in a step shape, and the droplet generation channel 231 is in a trumpet shape, so that droplets are formed by a step emulsification method.

The principle of the method for step floating is as follows:

first, a continuous phase (droplet forming oil) containing a surfactant fills the entire detection unit 20, and a sample (dispersed phase) flows along the microchannel 22 to the droplet forming space 23. As the sample (dispersed phase) passes the step formed by the droplet generation channel 231 and the droplet-spreading space 24, the dispersed phase forms a tongue structure in the nozzle (second channel 235) which, once it reaches the nozzle tip, expands and enters the wide and deep droplet-spreading space 24 to form a bubble structure. As the dispersed phase continues to be injected, the diameter of the bubble increases and the mean curvature decreases. To match the mean curvature of the bubble, the mean curvature of the dispersed phase fluid is reduced until a threshold of 2/h is reached due to the restriction of the nozzle, where h is the dimension of the second channel 235 in the thickness direction of the chip body 10. Thereafter, the equilibrium is broken as the dispersed phase no longer matches the bubble mean curvature reduction. The cross-sectional length of the neck decreases with increasing necking zone. When reduced to the height h of the second channel 235, the bubble breaks away from the dispersed phase fluid due to Rayleigh-Plateau instability, the droplet is released into the droplet tiling space 24, and the dispersed phase shrinks to the second channel 235 to form the next droplet.

In one embodiment, the size of the droplet generation channel 231 in the thickness direction is set to be 50 μm, the size of the droplet tiling space 24 in the thickness direction is set to be 100 μm, and the droplet tiling space 24 is set to be a size capable of accommodating at least twenty thousand droplets that are uniform and stable in size (100 μm in diameter). It is contemplated that in other embodiments, the dimensions of the droplet generation channel 231 and the droplet tiling space 24 in the thickness direction are not particularly limited. If the size of the droplet generation channel 231 in the thickness direction is 50 μm, the size of the droplet tiling space 24 in the thickness direction is 150 μm.

Further, first channel 232 comprises a first sub-channel 233 and a second sub-channel 234, first sub-channel 233 is communicated with sample port 21, and second sub-channel 234 is communicated with first sub-channel 233 and second channel 235. The first sub-passage 233 has a sectional area gradually increasing from one end to the other end communicating with the second sub-passage 234. In this way, the first sub-channel 233 is caused to flare out to facilitate the flow of liquid from the microchannel 22 into the droplet generation space 23.

In one embodiment, the oil discharge space 25 includes a plurality of oil discharge channels 251, and all the oil discharge channels 251 have the same cross-sectional shape. The size of the oil discharge channel 251 in the thickness direction of the chip body 10 is smaller than the size of the droplet tiling space 24. Thus, a step is formed between the oil discharge channel 251 and the droplet tiling space 24, and then the droplets located in the droplet tiling space 24 cannot flow to the oil discharge channel 251 due to the restriction of the step, and only the excess liquid is allowed to flow from the oil discharge channel 251 to the waste liquid storage space 26.

Further, each oil discharge channel 251 has the same sectional area from one end communicating with the droplet tiling space 24 to the other end. In other embodiments, the cross-sectional area of each oil discharge channel 251 may be different from one end communicating with the droplet tiling space 24 to the other end.

Referring to fig. 2, the microfluidic chip 100 further includes a filter element 30, and a filter element 30 is disposed in each of the exhaust ports 27, and the filter element 30 is sealed when meeting a liquid. When the filter element 30 is wetted with liquid from the waste liquid storage space 26, the filter element 30 is closed, and the liquid cannot flow in the detection unit 20 since the detection unit 20 is isolated from the outside. By the arrangement, only a quantitative sample can be added into the detection unit 20, so that the purpose of quantitative detection is achieved. Meanwhile, when the plurality of detection units 20 share one exhaust port 27, the step of the exhaust port 27 and the oil phase block the communication between the detection units 20, and meanwhile, the filter element 30 also plays a role in blocking the communication between the detection units 20, so that the risk of sample cross contamination is reduced.

In an embodiment, referring to fig. 1 and fig. 2, the microfluidic chip 100 further includes a sealing film 40, and the sealing film 40 is configured to be disposed on the chip body 10 to seal the sample loading port 21. After the sample is loaded, the sealing film 40 seals the sample loading port 21, so that the risk of cross contamination of each sample is reduced, and the biohazard is reduced.

The utility model provides a micro-fluidic chip 100's theory of operation as follows:

in this embodiment, the microfluidic chip 100 has 16 detection units 20, and can simultaneously complete the detection of 16 samples. The droplet generation channel 231 has a size of 50 μm in the thickness direction, and the droplet tiling space 24 has a size of 100 μm in the thickness direction.

A certain amount of droplet-forming oil is supplied from the supply port 21 to the detection unit 20, and the droplet-forming oil spreads over the entire droplet-spreading space 24. The mixture solution of the extracted nucleic acid solution and PCR reagent is fed from the sample port 21 to the detection unit 20, a predetermined positive pressure is applied from the sample port 21 or a predetermined negative pressure is extracted from the exhaust port 27, and the sample in the sample port 21 is driven by the above-mentioned pressure to enter the droplet-forming space 23 through the microchannel 22. Under the action of the step emulsification, the droplet generation space 23 generates fine droplets and spreads the droplets in the droplet spreading space 24, and the droplets are driven by the pressure to move toward the oil discharge space 25 until the droplet spreading space 24 is spread with the fine droplets (the droplet spreading space 24 is spread with at least twenty thousand fine droplets having a diameter of 100 μm). Excess waste liquid passes from the drain space 25 into the waste liquid storage space 26 and the vent 27 is closed as the liquid flows to the cartridge 30. And finally, matching with a laser confocal imaging system, performing optical data collection and analysis on the sample subjected to the PCR reaction in the droplet tiled space 24, and not needing to perform single detection on the droplets like a flow cytometer.

The utility model provides a micro-fluidic chip has following beneficial effect:

1. the sample inlets 21 of all the detecting units 20 are located on a first virtual circle, the exhaust outlets 27 of all the detecting units 20 are located on a second virtual circle, and the first virtual circle and the second virtual circle are nested with each other. Thus, the layout of the detecting units 20 on the chip body 10 is optimized, the space on the chip body 10 is reasonably utilized, and the micro-fluidic chip 100 can be further miniaturized under the condition that the same number of detecting units 20 are distributed.

2. The chip body 10 is disc-shaped, and the first virtual circle and the second virtual circle are concentric with the chip body 10, so as to further optimize the structure of the chip body 10 and achieve the purpose of further miniaturizing the microfluidic chip 100.

3. The microfluidic chip 100 includes a plurality of sets of detecting units sequentially arranged at intervals in the circumferential direction of the chip body 10, each set of detecting units includes at least two detecting units 20, and all detecting units 20 included in each set of detecting units share one air outlet 27. Thus, the structure of the detection unit 20 is optimized, and the microfluidic chip 100 can be further miniaturized with respect to the case where one exhaust port 27 is independently provided for each detection unit 20.

4. The micro-fluidic chip 100 has a simple structure, the size of the droplet generation channel 231 in the thickness direction is 50 μm, the size of the droplet tiling space 24 in the thickness direction is 100 μm, the size of the droplet tiling space 24 can accommodate at least twenty thousand droplets with uniform and stable size (the diameter is 100 μm), the obtained number of droplets is large, and the detection accuracy is improved.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims (10)

1. The microfluidic chip is characterized by comprising a chip body (10), wherein a plurality of detection units (20) are arranged on the chip body (10), and each detection unit (20) comprises a sample adding port (21), a liquid drop generation space (23), a liquid drop tiling space (24), an oil discharge space (25), a waste liquid storage space (26) and an exhaust port (27) which are sequentially communicated; the sample adding port (21) is used for adding a sample, the liquid drop generating space (23) is used for converting the sample into liquid drops and flatly laying the liquid drops in the liquid drop flatting space (24), the oil discharging space (25) is used for guiding waste liquid to the waste liquid storage space (26), and the air discharging port (27) is used for exhausting air during sample adding;

wherein the sample addition ports (21) of all the detection units (20) are positioned on a first virtual circle, the exhaust ports (27) of all the detection units (20) are positioned on a second virtual circle, and the first virtual circle and the second virtual circle are nested with each other.

2. Microfluidic chip according to claim 1, characterized in that at least two of said detection units (20) share one of said gas vents (27).

3. The microfluidic chip according to claim 2, wherein the microfluidic chip comprises a plurality of sets of detection units sequentially arranged at intervals in a circumferential direction of the chip body (10), each set of detection units comprising at least two detection units (20);

all the detection units (20) included in each group of the detection unit groups share one exhaust port (27).

4. The microfluidic chip according to claim 3, wherein all the detecting units (20) included in each group of detecting units meet at the exhaust port (27) from two circumferential sides of the chip body (10).

5. The microfluidic chip according to claim 1, wherein the chip body (10) is disc-shaped, and the first virtual circle and the second virtual circle are both concentric with the chip body (10).

6. The microfluidic chip according to claim 1, wherein the droplet generation space (23) comprises a plurality of droplet generation channels (231) connected between the loading port (21) and the droplet tiling space (24), and all the droplet generation channels (231) have the same cross-sectional shape; the size of the droplet generation channel (231) in the thickness direction of the chip body (10) is smaller than the size of the droplet tiling space (24);

each droplet generation channel (231) comprises a first channel (232) and a second channel (235) which are communicated with each other, the first channel (232) is communicated with the sample loading port (21), and the second channel (235) is communicated with the droplet tiling space (24);

the second passage (235) has a sectional area gradually increasing from one end communicating with the first passage (232) to the other end.

7. The microfluidic chip according to claim 6, wherein the first channel (232) comprises a first sub-channel (233) and a second sub-channel (234), the first sub-channel (233) is in communication with the sample port (21), and the second sub-channel (234) is in communication with the first sub-channel (233) and the second channel (235);

the cross-sectional area of the first sub-passage (233) communicating with the second sub-passage (234) is gradually increased from one end to the other end.

8. The microfluidic chip according to claim 1, wherein the oil drain space (25) includes a plurality of oil drain channels (251), all of the oil drain channels (251) having the same cross-sectional shape;

the size of the oil discharge channel (251) in the thickness direction of the chip body (10) is smaller than the size of the droplet tiling space (24).

9. The microfluidic chip according to claim 1, further comprising a filter element (30), wherein the filter element (30) is disposed in each of the gas outlets (27), and the filter element (30) is sealed in the presence of a liquid.

10. The microfluidic chip according to any of claims 1 to 9, further comprising a sealing membrane (40), wherein the sealing membrane (40) is configured to be capable of being placed on the chip body (10) for sealing the sample addition port (21).

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