CN217521029U - Multi-dimensional imaging device for identifying plankton - Google Patents

Multi-dimensional imaging device for identifying plankton Download PDF

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Publication number
CN217521029U
CN217521029U CN202221340839.6U CN202221340839U CN217521029U CN 217521029 U CN217521029 U CN 217521029U CN 202221340839 U CN202221340839 U CN 202221340839U CN 217521029 U CN217521029 U CN 217521029U
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capillary flow
plankton
sample
blind hole
identification
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万峻
董晓伟
王珊珊
刘东艳
王玉珏
何乃文
王龙
赵国鹏
隋晓飞
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Beijing Aoshi Technology Co ltd
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Beijing Aoshi Technology Co ltd
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Abstract

The utility model discloses a multi-dimensional image device for plankton appraisal, include: a base; a plurality of capillary flow tubes in which a sample to be detected is carried; an object stage disposed on the base; the blind hole is formed on the objective table, and the lower end of any capillary flow tube is detachably inserted into the blind hole; the objective lens disc is arranged on the base in a liftable mode and comprises two annular bodies; at least three multiple compound objective lenses; and a microscopic camera in communication with the at least three multiple compound objective lenses. The utility model provides a multidimensional imaging device for plankton appraisal, it utilizes a plurality of capillary flow tubes and combines at least three multifold compound objective to carry out three-dimensional multidimension panorama formation of image, realizes the multidimension high-resolution formation of image to the plankton to show improvement formation of image and recognition effect, with the categorised research of supplementary high accuracy that realizes the plankton.

Description

Multi-dimensional imaging device for identifying plankton
Technical Field
The utility model relates to a plankton on-line measuring technical field, in particular to a multidimensional imaging device for plankton appraisal.
Background
Phytoplankton refers to tiny organisms living in marine, lake, river and other water bodies and is a very important biological group in aquatic ecosystems. Parameters such as the species, biomass and biological diversity of phytoplankton are important indexes reflecting aquatic ecological health, and have clear requirements in relevant guidelines and specifications of river, lake and ocean monitoring and ecological health evaluation in China.
The existing phytoplankton on-line identification technology can not meet the requirements of quick, fine and accurate full-automatic monitoring, the grade of identification species is not high, the ecological effect between the phytoplankton and the water environment can not be reflected in time, and the scientific support of water ecological early warning and environment supervision in China is restricted. The first research method of phytoplankton is to use an optical microscope to directly observe to obtain required data, but the observation by the microscope needs complicated manual operation and professional biological classification knowledge, is time-consuming and labor-consuming, is easy to cause errors due to subjective judgment of an observer, cannot accurately reflect the health condition of a water body in time, and particularly when a research area is large and involves space and time, a microscopic examination method is greatly limited, so that the analysis of a sample is slow, and further, the evaluation of an ecological environment system is delayed. The existing developed technology for quickly detecting phytoplankton is a Flow cytometry and microscopy (FlowCAM), but the instrument also faces a great problem in application, namely firstly, the phytoplankton has a wide particle size range and is easy to aggregate into a cluster or a chain, and the characteristic of Flow sample detection of the phytoplankton easily causes blockage of a Flow cell and cross contamination among different samples; secondly, the phytoplankton is various in types and shapes, and the accuracy of digital image recognition of single-angle shooting needs to be improved.
In conclusion, the existing imaging method cannot realize three-dimensional multi-dimensional identification of phytoplankton, has low resolution, is difficult to meet the objective requirement of accurate identification and classification of the phytoplankton, and seriously restricts the scientific research progress of the analysis of the composition characteristics of phytoplankton species in the aquatic ecosystem in China.
Disclosure of Invention
It is an object of the present invention to solve at least the above problems and to provide at least the advantages which will be described later.
The utility model discloses it is still another purpose to provide a multidimensional imaging device for plankton appraisal, it utilizes a plurality of capillary flow tubes and combines at least three multifold composite objective to carry out three-dimensional multidimension panorama formation of image, realizes the multidimension high-resolution formation of image to the plankton to show improvement formation of image and recognition effect, with the categorised research of the high accuracy of supplementary realization plankton.
To achieve these objects and other advantages in accordance with the purpose of the invention, there is provided a multi-dimensional imaging device for plankton identification, including:
a base;
a plurality of capillary flow-through tubes in which a sample to be tested is carried;
an object stage disposed on the base; the blind hole is formed on the objective table, and the lower end of any capillary flow-through pipe is detachably inserted into the blind hole;
the objective lens disc is arranged on the base in a liftable mode and comprises two annular bodies;
the object lens disc is uniformly distributed around the object stage in a divergent manner by taking any point on an extension line where the blind hole is located as a circle center, the at least three multi-fold compound objects are clamped between the two annular bodies, the plane where the at least three multi-fold compound objects are located is perpendicular to the extension line where the blind hole is located, and the optical axes of the at least three multi-fold compound objects are intersected at any point on the extension line where the blind hole is located; and
and the microscopic camera is in communication connection with the at least three multi-time composite objective lenses, the at least three multi-time composite objective lenses and the objective lens disc synchronously ascend and descend, and synchronously collects plankton multi-dimensional image sets in any capillary flow tube inserted in the blind hole through the microscopic camera.
Preferably, the objective lens disk and the stage are made of transparent materials, and the capillary flow tubes are made of quartz materials.
Preferably, the depth of the blind hole is less than or equal to 1/9 of the length of any capillary flow-through tube; the volume of any capillary flow-through tube is more than or equal to 0.1 ml.
Preferably, the depth of the blind hole is equal to 1/9 of the length of any capillary flow-through tube; the volume of any capillary flow-through is 0.1ml, 0.5ml or 1 ml.
Preferably, the objective lens disk further comprises: the upright post is vertically fixed on the base; and one end of the sliding block is fixed on the two annular bodies, and the other end of the sliding block is arranged on the stand column in a sliding manner.
Preferably, the method further comprises: the sample pool is communicated to a water body to be detected through a sample inlet pipe;
the peristaltic pump and the filter are arranged on the sampling pipe;
the supporting plate is uniformly distributed with a plurality of blind holes II, a plurality of capillary circulation pipes are uniformly inserted into the blind holes II at intervals, and the supporting plate is arranged close to the sample cell; and
and the recovery plate is uniformly distributed with a plurality of blind holes III, and a plurality of capillary flow tubes with detected samples are inserted into the blind holes III on the support plate in a one-to-one correspondence manner.
Preferably, the method further comprises the following steps: one end of the waste liquid outlet pipe is communicated to the bottom of the sample pool, and the other end of the waste liquid outlet pipe extends outwards; the sampling pipes comprise a first sampling pipe arranged on the water inlet side of the peristaltic pump and a second sampling pipe arranged on the outlet side of the peristaltic pump;
the three-way valve is arranged between the first sample inlet pipe and the peristaltic pump, a first water inlet of the three-way valve is communicated to a sample outlet of the first sample inlet pipe, and a water outlet of the three-way valve is communicated to a water inlet of the peristaltic pump; and
the cleaner comprises a storage cavity, purified water is prestored in the storage cavity, a water outlet of the storage cavity is communicated with a second water inlet of the tee joint through a water outlet pipe, and the driving portion drives the cleaner to clean at regular time.
The utility model discloses at least, include following beneficial effect:
the utility model utilizes a plurality of capillary flow tubes and combines at least three multifold composite objective lenses to carry out three-dimensional multi-dimensional panoramic imaging, thus realizing multi-dimensional high-resolution imaging of plankton, obviously improving imaging and recognition effects and providing image set materials for realizing high-precision classification of plankton;
in practical application, an online monitoring mode can be established by using the technology of the internet of things, and an image set in the microscope camera can be quickly and conveniently acquired and stored in time through mobile networks such as 4G/5G.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Drawings
Fig. 1 is a schematic structural diagram of a multidimensional imaging device for identifying plankton in an embodiment of the present invention;
fig. 2 is a schematic structural view of a tee joint and a tee joint according to an embodiment of the present invention;
fig. 3 is a schematic structural diagram of a system for multi-dimensional identification using the multi-dimensional imaging device for plankton identification according to the present invention in one embodiment;
fig. 4 is a flow chart of multi-dimensional identification by using the multi-dimensional imaging device for identifying plankton in another embodiment.
Detailed Description
The present invention is further described in detail below with reference to the drawings so that those skilled in the art can implement the invention with reference to the description.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
As shown in fig. 1, the present invention provides a multi-dimensional imaging device for plankton identification, comprising:
a base 1; a plurality of capillary flow channels 201 (201') in which a sample to be tested is carried; an object stage 401 provided on the base; a blind hole 4011 formed on the objective table, wherein the lower end of any capillary flow-through tube is detachably inserted into the blind hole; an objective lens disk 403 which is arranged on the base in a liftable manner, wherein the objective lens disk comprises two annular bodies; at least three multi-fold compound objective lenses 402, which are uniformly distributed around the objective table in a divergent manner by taking any point on the extension line of the blind hole as the center of a circle through an objective lens disc, are clamped between the two annular bodies, the plane of the at least three multi-fold compound objective lenses is vertical to the extension line of the blind hole, and the optical axes of the at least three multi-fold compound objective lenses are intersected at any point on the extension line of the blind hole; and the microscopic camera is in communication connection with the at least three multiple compound objective lenses, the at least three multiple compound objective lenses and the objective lens disc synchronously ascend and descend, and the microscopic camera is used for synchronously acquiring a plankton multidimensional image set in any capillary flow tube inserted in the blind hole. In the scheme, the whole multi-dimensional imaging device for identifying plankton comprises a plurality of capillary flow tubes for bearing a sample to be detected, an objective table, an objective disc, at least three multiple compound objectives and a microscopic camera; at least three compound objective uses the objective disc as the support, evenly sends out and scatters the setting, and the plankton in the sample that awaits measuring in a pair of capillary flow tube carries out synchronous omnidirectional shooting to obtain the 360 panorama of same plankton, provide clear and omnidirectional image set for software or procedure etc. that later stage carries out the appraisal to the plankton in this sample that awaits measuring, with the appraisal precision that effectively improves appraisal software.
In conclusion, as the samples are stored in the capillary flow tube independently, the technical problems that the flow cell is easy to block and the cross contamination among different samples is easy to cause in the application of the current flow imager method are solved, and unexpected technical effects are obtained. In practical application, the capillary flow-through tube can be manually inserted into the blind hole or a mechanical arm is arranged to grab the capillary flow-through tube.
In a preferred embodiment, the objective table and the objective disk are made of transparent materials, such as: organic glass, quartz glass, etc. and the capillary flow tubes are made of quartz material. In this scheme, objective table and objective disc are transparent material, even if insert the sample in the capillary flow tube lower extreme of blind hole and also can carry out microscopic observation and shoot, guarantee to detect accurately.
In a preferred embodiment, the depth of the blind hole is less than or equal to 1/9, such as 1/9, 1/10 or 1/12; the volume of any capillary flow-through is 0.1ml or more, such as 0.1ml, 0.5ml, 1ml or 2 ml. In the scheme, the capillary flow tubes with different volumes can meet the detection requirements of different samples, and are suitable for various practical detection application scenes.
In a preferred scheme, the depth of the blind hole is equal to 1/9 of the length of any capillary flow-through tube; the volume of any capillary flow-through is 0.1ml, 0.5ml or 1 ml.
In a preferred embodiment, the objective lens disk further comprises: a column 405 vertically fixed on the base; and a sliding block 404, one end of which is fixed to the two annular bodies and the other end of which is slidably arranged on the upright post. The objective lens disk is arranged on the upright post through the sliding block in a sliding way so as to realize the up-and-down stable movement of the objective lens disk.
In a preferred embodiment, the method further comprises: the sample pool is communicated to a water body to be detected through a sample inlet pipe; the peristaltic pump and the filter are arranged on the sampling pipe; pumping a sample to be detected into a sample pool by a peristaltic pump and a filter, filtering, removing impurities with larger particles (such as gravel with the diameter of more than or equal to 1 mm), and avoiding blocking a capillary flow tube; the supporting plate is uniformly distributed with a plurality of blind holes II, a plurality of capillary circulation pipes are uniformly inserted into the blind holes II at intervals, and the supporting plate is arranged close to the sample cell; and the recovery plate is uniformly provided with a plurality of blind holes III, and a plurality of capillary flow tubes with detected samples are correspondingly inserted into the blind holes III on the support plate one by one. In the scheme, the capillary flow tubes can be effectively separated and fixed on the supporting plate and the recovery plate, so that cross infection of samples is avoided, and the accuracy of detection results is ensured; the depth of the blind holes II or the blind holes III is smaller than the length of the capillary flow-through tube, so that the insertion or the removal operation is convenient.
As shown in fig. 2, in a preferred embodiment, the method further includes: a waste liquid outlet pipe 1011 having one end connected to the bottom of the sample cell 101 and the other end extending outward; the sampling pipes comprise a first sampling pipe 1012 arranged on the water inlet side of the peristaltic pump and a second sampling pipe 1013 arranged on the outlet side of the peristaltic pump; the tee joint 104 is arranged between the first sample inlet pipe and the peristaltic pump, a first water inlet of the tee joint is communicated to a sample outlet of the first sample inlet pipe, and a water outlet of the tee joint is communicated to a water inlet of the peristaltic pump; and the cleaner 105 comprises a storage cavity, purified water is prestored in the storage cavity, a water outlet of the storage cavity is communicated with a second water inlet of the tee joint through a water outlet pipe, and the driving part drives the cleaner to clean at regular time. In the scheme, a three-way connection sample pool, a peristaltic pump, a sample bottle 106 and a cleaner are arranged, when a sample needs to be fed, a water outlet pipe of the cleaner is closed, only the sample is fed, and after detection of one sample is finished, waste liquid is discharged through a waste liquid discharge pipe; and then, starting the cleaner again, pumping the purified water in the storage cavity into the sample cell through the peristaltic pump, and cleaning the sample tube and the sample cell, so that the pollution among samples can be effectively avoided.
Example 1
In practical application, the multidimensional imaging device for identifying plankton provided by the present application can be provided with a matching component for performing automatic identification operation of plankton, as shown in fig. 3, a base 1; the sampling assembly 10 is arranged on the base and comprises a sample pool 101, and the sample pool is communicated to a water body to be detected through a sample inlet pipe 102; a peristaltic pump 103 and a filter, both disposed on the sample inlet tube; a sample preparation assembly 20 disposed on the base, the sample preparation assembly comprising a plurality of capillary flow tubes 201, which are uniformly spaced apart and inserted on a sample stage 202, the sample stage being disposed adjacent to the sample cell; a recovery stage 203 on which a plurality of capillary flow-through tubes 201' with the detected sample are detachably inserted; a mechanical transfer assembly 30 including an XY axis moving stage 301 disposed on the base; a mechanical arm 302 which is arranged on the XY axis moving platform, the front end of the mechanical arm is provided with a clamp 3021, and the front end of the clamp is detachable to clamp any capillary flow-through tube; the clamp comprises two clamping pieces which are hinged; an optical imaging assembly 40 comprising a stage 401 disposed on a base; the blind hole is formed on the objective table, and the lower end of any capillary flow-through pipe is detachably inserted into the blind hole; an objective lens disk 403 which is arranged on the base in a lifting way; at least three multiple compound objective lenses 402, which are divergently and uniformly distributed around the objective table through the objective lens disc by taking any point on the extension line where the blind hole is located as the center of a circle, the plane where the at least three multiple compound objective lenses are located is perpendicular to the extension line where the blind hole is located, and the optical axes of the at least three multiple compound objective lenses are intersected at any point on the extension line where the blind hole is located; the microscopic camera is in communication connection with the at least three multi-time composite objective lenses, the at least three multi-time composite objective lenses and the objective lens disc synchronously ascend and descend, and plankton multi-dimensional image sets inserted in any capillary flow tube on the blind hole are synchronously acquired through the microscopic camera; in practical application, the devices can be integrated in a shell, so that sample pollution and the like are avoided; the driving assembly is used for respectively driving the peristaltic pump, the XY axis moving platform, the mechanical arm, the microscopic camera and the objective lens to be in disc control; the control center is in wireless communication connection with the microscopic camera and the driving assembly, and further comprises an image library, wherein a classification atlas of plankton samples is prestored in the image library; and the analysis module is used for acquiring the plankton multidimensional image set shot by the microscopic camera in real time, then comparing and analyzing the plankton multidimensional image set with the classification image set of the image library, and returning an analysis result. In the scheme, the whole online automatic identification system for the plankton multidimensional imaging comprises a sample collection unit (a sampling assembly), a sample preparation unit (a sample preparation assembly), an optical imaging unit (at least three multiple compound objective lenses and a microscope camera), an analysis processing unit (an analysis module) and a core control unit (a control center and a driving assembly). A plurality of capillary flow tubes which can meet the use requirement of a maintenance period are pre-arranged in a sample preparation unit (for example, one maintenance period is a quarter, or a month, sampling is carried out every quarter or a month, after each sampling, each sample detects two tubes (two capillary flow tubes are adopted), the results of the two tubes are averaged, if the error is more than 15%, the sample detects one tube more, and if the number of sampling products is 10, at least 30 capillary flow tubes are arranged in each maintenance period); the driving component drives the XY-axis moving platform to operate, after the mechanical arm is driven to move to a proper position, the front end of the mechanical arm extends to the upper end of a capillary flow pipe and is clamped by a clamp to be lifted, the lower end of the capillary flow pipe extends into a sample in the sample pool, the suspension is carried out for a certain time, after the capillary flow pipe automatically absorbs the sample, the mechanical arm is lifted, the XY-axis moving platform drives the mechanical arm to move to a position right opposite to the objective table, the mechanical arm inserts the lower end of the capillary flow pipe into the blind hole to be fixed, then, the driving component drives the animal lens disc to slowly ascend or descend, so that 360-degree multi-dimensional observation is carried out on the sample in the capillary flow pipe through at least three multi-fold composite objective lenses, and a panoramic picture of each biological particle is obtained through the microscope camera; the microscopic camera receives and stores a plankton multidimensional image set (panoramic picture) for analysis, comparison or classification, density calculation and the like by an analysis module; and a multiple compound objective lens in the optical imaging unit transmits the sample in the capillary flow tube to an analysis processing unit, and identification and analysis software automatically classifies each picture and counts the number or density of each phytoplankton. All units are integrally controlled by a control center, and data results are transmitted to a data platform appointed by a user through the Internet of things. And the identified capillary flow-through tube is moved to a recovery platform by the mechanical arm and the XY-axis moving platform for check.
Example 2
In practical application, can set up the supporting subassembly to the multidimensional imaging device for plankton appraisal that this application provided and carry out the automatic appraisal operation of plankton, as shown in fig. 4, (1) the sample collection unit, the function of sample collection unit is to collecting and the pretreatment to the water sample, is the basic link that realizes the automatic appraisal of phytoplankton. The peristaltic pump technology is adopted, the minimum water inflow can be as low as 0.01mL, and the automatic collection function of a trace water sample is realized through the core control unit.
The sample collection unit comprises a peristaltic pump, a pipeline, a filter, a sample pool, a cleaner and a sensor. The peristaltic pump collects a water sample to the filter through the pipeline, and the filtered water sample is collected to the sample pool for the sample preparation unit to prepare. The system can set a certain time period to clean the sample cell, the automatic cleaning of the sample cell can be realized through the cleaning device, meanwhile, the sensor can detect one or more parameters such as the water temperature of the sample cell, and the parameters can be stored together with a final test result, so that the system provides help for analysis work. In order to better ensure the living environment of plankton, the sample collection unit can also be equipped with a thermostat to balance the water temperature of the sample pool.
(2) The sample preparation unit is a main unit for embodying the automatic quantitative preparation of the sample, the sample is made into a state which can be used for visual observation, and the core element of the sample preparation unit is a cylindrical capillary flow tube with fixed volume and is made of transparent quartz material. The two ends of each flow tube are marked with numbers to correspond to the sample numbers. The sample preparation unit is pre-loaded with a fixed volume (e.g., 0.1ml, 0.5ml, 1ml, etc.) of capillary flow-through tubes for a maintenance cycle, one capillary flow-through tube for each monitoring. The mechanical conveying device grabs the capillary flow-through tube and sucks the sample into the capillary flow-through tube, and after the suction is finished, the capillary flow-through tube is placed on the supporting plate for visual observation. And after the observation is finished, the capillary flow tube enters the recovery plate to be sealed and recovered into the recovery box.
The application innovatively adopts the capillary flow tube to load the sample, is the best scheme for cooperating with the annular objective lens disk to observe, is also suitable for the optical angle of 360-degree multi-dimensional observation, and can show the three-dimensional posture of the particles without damage. When in observation, the objective disc drives at least three times of composite objectives to uniformly translate around the capillary flow tube, and a water sample is relatively static in the capillary flow tube, so that all samples can be completely observed, and the problem of blockage of the flow cell is avoided.
(3) The optical imaging unit (at least three multiple compound objective lenses and a micro camera), the sample preparation unit is a main unit for embodying the automatic quantitative preparation of the sample, the sample is made into a state capable of being used for visual observation, and the core element of the optical imaging unit is a columnar capillary flow tube with fixed volume and made of transparent quartz material. The two ends of each flow tube are marked with numbers to correspond to the sample numbers. The sample preparation unit is pre-provided with capillary flow-through tubes which can be used for one maintenance cycle, the sample loading amount is a fixed volume (such as 0.1ml, 0.5ml, 1ml and the like), and one capillary flow-through tube is used for each monitoring. The mechanical conveying device grabs the capillary circulation tube and sucks the sample into the capillary circulation tube, and after the suction is finished, the capillary circulation tube is placed on a support plate (an objective table) for visual observation. And after the observation is finished, the capillary flow tube enters the recovery plate to be sealed and recovered into the recovery box.
(4) And an analysis processing unit (which adopts the existing analysis module for analysis), wherein the analysis processing unit refers to identification software and a biological species library. The establishment of the species library is that plankton samples of different water bodies are collected and purchased, and determined species images are input into identification software through dual identification of an identification instrument and a traditional microscope, so that automatic classification of observation samples is realized. The establishment of plankton libraries can not only realize the identification of samples rapidly, but also have profound significance for the classification work of aquatic organisms in different water bodies in China.
The identification software comprises an instrument control function, an image acquisition function, an image analysis function, a data storage function, a result reporting function and a network transmission function. The control function of the instrument realizes the control of each module of the instrument, and the Ethernet, RS-232, RS-485 and other buses are adopted for communication and transmission. The communication protocol is established according to each module. The image acquisition function is to acquire images in real time through an acquisition interface provided by the high-definition micro-camera, and the acquired images are displayed on a software main interface. The image analysis function is to perform real-time or selective analysis on the acquired images through various algorithms, and can perform operations such as screening, database comparison and the like according to conditions set by a user. The data storage function is to store the acquired images and analysis results into a database. The result reporting function can generate professional report templates, can freely set report contents and formats, and can export Excel or Word document formats. The network transmission function provides an interface for the cloud platform, and the detection result 'cloud on key' can be butted with various cloud platforms.
(5) Core control unit (control center and drive assembly). The core control unit carries out systematic control on the units, and the guarantee for realizing logic operation and automation is realized. The unit comprises an embedded system, a motor driver, a signal collector and a switching value controller. The embedded system runs the core control software to control the whole instrument. The motor driver is responsible for the action of controlling mechanical transmission device (XY axle moving platform and arm), and signal collector is responsible for gathering various signals, contains: photoelectric switch, Hall element, temperature, etc. and the switching value controller is responsible for controlling various on-off. These modules constitute the core control unit.
While the embodiments of the invention have been described above, it is not intended to be limited to the details shown, or described, but rather to cover all modifications, which would come within the scope of the appended claims, and all changes which come within the meaning and range of equivalency of the art are therefore intended to be embraced therein.

Claims (7)

1. A multi-dimensional imaging apparatus for plankton identification, comprising:
a base;
a plurality of capillary flow tubes in which a sample to be detected is carried;
an object stage disposed on the base; the blind hole is formed on the objective table, and the lower end of any capillary flow-through pipe is detachably inserted into the blind hole;
the objective lens disc is arranged on the base in a liftable mode and comprises two annular bodies;
the object lens disc is uniformly distributed around the object stage in a divergent manner by taking any point on an extension line where the blind hole is located as a circle center, the at least three multi-fold compound objects are clamped between the two annular bodies, the plane where the at least three multi-fold compound objects are located is perpendicular to the extension line where the blind hole is located, and the optical axes of the at least three multi-fold compound objects are intersected at any point on the extension line where the blind hole is located; and
and the microscopic camera is in communication connection with the at least three multi-time composite objective lenses, the at least three multi-time composite objective lenses and the objective lens disc synchronously ascend and descend, and synchronously collects plankton multi-dimensional image sets in any capillary flow tube inserted in the blind hole through the microscopic camera.
2. The multi-dimensional imaging device for plankton identification of claim 1, wherein the stage and objective disk are transparent and the plurality of capillary flow channels are made of quartz.
3. The multi-dimensional imaging device for plankton identification of claim 1, wherein the depth of the blind hole is less than or equal to 1/9 of the length of any capillary flow-through tube; the volume of any capillary flow-through tube is more than or equal to 0.1 ml.
4. The multi-dimensional imaging device for planktonic identification of claim 1 wherein the depth of the blind hole is equal to 1/9 times the length of any capillary flow-through; the volume of any capillary flow-through is 0.1ml, 0.5ml or 1 ml.
5. The multi-dimensional imaging apparatus for plankton identification of claim 1, wherein the objective lens tray further comprises:
the upright post is vertically fixed on the base; and one end of the sliding block is fixed on the two annular bodies, and the other end of the sliding block is arranged on the stand column in a sliding manner.
6. The multi-dimensional imaging apparatus for plankton identification of claim 1, further comprising:
the sample pool is communicated to a water body to be detected through a sample inlet pipe;
the peristaltic pump and the filter are arranged on the sampling pipe;
the supporting plate is uniformly distributed with a plurality of blind holes II, a plurality of capillary circulation pipes are uniformly inserted into the blind holes II at intervals, and the supporting plate is arranged close to the sample cell; and
and the recovery plate is uniformly distributed with a plurality of blind holes III, and a plurality of capillary flow tubes with detected samples are inserted into the blind holes III on the support plate in a one-to-one correspondence manner.
7. The multi-dimensional imaging apparatus for plankton identification of claim 6, further comprising:
one end of the waste liquid outlet pipe is communicated to the bottom of the sample pool, and the other end of the waste liquid outlet pipe extends outwards; the sampling pipes comprise a first sampling pipe arranged on the water inlet side of the peristaltic pump and a second sampling pipe arranged on the outlet side of the peristaltic pump;
the three-way valve is arranged between the first sample inlet pipe and the peristaltic pump, a first water inlet of the three-way valve is communicated with a sample outlet of the first sample inlet pipe, and a water outlet of the three-way valve is communicated with a water inlet of the peristaltic pump; and
the cleaner comprises a storage cavity, purified water is prestored in the storage cavity, a water outlet of the storage cavity is communicated with a second water inlet of the tee joint through a water outlet pipe, and the driving portion drives the cleaner to clean at regular time.
CN202221340839.6U 2022-05-31 2022-05-31 Multi-dimensional imaging device for identifying plankton Active CN217521029U (en)

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