CN217516948U - Phage separation device for phage culture - Google Patents
Phage separation device for phage culture Download PDFInfo
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- CN217516948U CN217516948U CN202220516687.4U CN202220516687U CN217516948U CN 217516948 U CN217516948 U CN 217516948U CN 202220516687 U CN202220516687 U CN 202220516687U CN 217516948 U CN217516948 U CN 217516948U
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Abstract
The disclosure belongs to the field of phage culture and separation, and particularly relates to a phage separation device for phage culture; the device comprises a supporting plate, wherein a sealing bin is arranged on the supporting plate, the sealing bin is columnar, and the upper end of the sealing bin is open; the sealing bins are arranged in a plurality of numbers; a fixed sampling tube is arranged in the sealed bin; one end of the sealed bin is provided with a breather pipe which is connected with an air chamber; the bottom end of the sealed bin is provided with a rotating block which rotates around the axis of the rotating block, and the rotating block is provided with a plurality of adsorption holes; used for carrying out phage separation on phage culture samples.
Description
Technical Field
The disclosure belongs to the field of phage culture and separation, and particularly relates to a phage separation device for phage culture.
Background
Phage isolation assays are separation and analysis techniques and methods employed to determine phage. Bacteriophages are viruses that parasitize bacteria, are widely distributed in nature, and are proliferated in large quantities after invading host cells to cause lysis thereof, which makes the culture fluid clear. Accordingly, the presence of phage can be analyzed and tested.
The common methods are as follows: the bacteria-free culture analysis method comprises mixing the sample with broth, culturing, centrifuging at 2500rpm/min for 15 min, collecting supernatant, filtering with bacteria filter, culturing the filtrate, and observing the presence of bacteria to determine the existence of bacteriophage.
SUMMERY OF THE UTILITY MODEL
In view of the shortcomings of the prior art, the present disclosure is directed to providing a phage separation device for phage culture.
The purpose of the disclosure can be realized by the following technical scheme:
a phage separation device for phage culture comprises a support plate, wherein a sealing bin is arranged on the support plate, the sealing bin is columnar, and the upper end of the sealing bin is open;
the sealing bins are arranged in a plurality of numbers;
a fixed sampling tube is arranged in the sealed bin;
one end of the sealed bin is provided with a breather pipe which is connected with an air chamber;
the bottom of sealed storehouse is provided with the rotatory piece that carries out the rotation around self axis, offers on the rotatory piece to have put a plurality of absorption holes.
Further, rotatory piece coaxial line fixed mounting is on first drive gear, and first drive gear rotates and installs in the backup pad, and first drive gear between a plurality of rotatory pieces passes through the hold-in range to be connected, is provided with second drive gear on the first drive gear, and second drive gear one side meshing has third drive gear, fixed connection between the output shaft of third drive gear and motor.
Furthermore, the lower end of the rotating block is provided with an adsorption channel which is connected in a rotating mode, a connector is arranged on the adsorption channel, and the connector is communicated with the negative pressure chamber through a pipeline.
Furthermore, a sealing cover is arranged at the upper end of the sealing bin and clamped and covered at the upper end of the sealing bin.
Furthermore, an electromagnetic valve for controlling the ventilation pipe to be switched on and off is arranged on the ventilation pipe.
Furthermore, an installation groove is formed in the side wall of the sealed bin, a telescopic rod is arranged in the installation groove, the bottom end of the telescopic rod is fixedly installed at the bottom end of the installation groove, a limiting block is arranged on an upper end rod of the telescopic rod, and a spring is sleeved on the telescopic rod at the lower end of the limiting block;
the lower end of the sealing cover is provided with a mounting hole, and an induction block is arranged in the mounting hole;
when sealed lid card lid was in sealed storehouse, the telescopic link upper end stretched into to the mounting hole in, until inconsistent with the response piece, the conflict signal to the telescopic link is responded to the response piece, will conflict signal transmission to controller, and the controller controls the solenoid valve.
Further, a sealing ring block is arranged at the upper end of the sealing bin.
Furthermore, a first exhaust hole is formed in the sealing ring block, a second exhaust hole is formed in the sealing cover, and when the sealing cover is clamped and covered in the sealing bin, the first exhaust hole is communicated with the second exhaust hole.
Further, when the telescopic rod extends into the mounting hole, the first exhaust hole is aligned with the second exhaust hole.
The beneficial effect of this disclosure:
the present disclosure enables phage isolation of phage culture samples.
Drawings
In order to more clearly illustrate the embodiments or technical solutions in the prior art of the present disclosure, the drawings used in the description of the embodiments or prior art will be briefly described below, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without creative efforts.
FIG. 1 is an overall schematic structural view of an embodiment of the present disclosure;
FIG. 2 is a schematic view from a different perspective of an embodiment of the present disclosure;
FIG. 3 is a partial structural schematic of an embodiment of the present disclosure;
FIG. 4 is an enlarged schematic view of FIG. 3 in accordance with an embodiment of the present disclosure;
FIG. 5 is a partial structural schematic of an embodiment of the present disclosure;
fig. 6 is a partial structural schematic of an embodiment of the present disclosure.
Detailed Description
The technical solutions in the embodiments of the present disclosure will be clearly and completely described below with reference to the drawings in the embodiments of the present disclosure, and it is obvious that the described embodiments are only a part of the embodiments of the present disclosure, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments disclosed herein without making any creative effort, shall fall within the protection scope of the present disclosure.
As shown in fig. 1 to 6, a phage separation device for phage culture comprises a support plate 1, wherein a sealing bin 2 is arranged on the support plate 1, the sealing bin 2 is columnar, and the upper end of the sealing bin 2 is open;
a fixed sampling tube 3 is arranged in the sealed bin 2;
one end of the sealed bin 2 is provided with a vent pipe 5, and the vent pipe 5 is connected with an air chamber 6;
the bottom of the sealed cabin 2 is provided with a rotating block 7 rotating around the axis of the rotating block, and the rotating block 7 is provided with a plurality of adsorption holes 70.
In this application, sealed storehouse 2 can set up to a plurality ofly to can save a plurality ofly for a single time and decide sampling tube 3, the solenoid valve controls 5 pipeline break-make of breather pipes of sealed storehouse 2 that correspond.
When the device is used, the fixed sampling tube 3 carries out quantitative sampling on the phage, if the sampling tube is used for carrying out quantitative sampling on the phage to the fixed sampling tube 3, the fixed sampling tube 3 is placed in the sealed bin 2, the air chamber 6 carries out ventilation on the sealed bin 2 through the ventilation pipe 5, the temperature in the sealed bin 2 is adjusted, so that the fixed sampling tube 3 is at the appropriate heat preservation temperature, the fixed sampling tube 3 is used for preservation, the adsorption hole 70 on the rotating block 7 adsorbs the bottom end of the fixed sampling tube 3, and at the moment, the bottom end of the fixed sampling tube 3 is adsorbed and fixed on the rotating block 7; when phage separation needs to be carried out on a sample in the fixed sampling tube 3, the rotating block 7 rotates to drive the fixed sampling tube 3 to rotate, so that the sample in the fixed sampling tube 3 is separated, supernatant obtained from the fixed sampling tube 3 is filtered by a bacteria filter, filtrate is cultured, and existence of the phage is judged by observing bacterial growth; the temperature of the gas introduced into the sealed cabin 2 belongs to the prior art, for example, the ventilation pipe 5 can be heated or cooled, for example, the ventilation pipe 5 is introduced into the existing temperature adjusting mechanism.
In terms of the rotation of the rotating blocks 7, in the present application, the rotating blocks 7 are coaxially and fixedly mounted on a first driving gear 81, the first driving gear 81 is rotatably mounted on the support plate 1, the first driving gears 81 among the plurality of rotating blocks 7 are connected through a timing belt 82, a second driving gear 83 is arranged on the first driving gear 81, a third driving gear 84 is engaged with one side of the second driving gear 83, and the third driving gear 84 is fixedly connected with an output shaft of a motor 85; when the sampling tube rotating device is used, the motor 85 drives the third driving gear 84 to rotate, the third driving gear 84 drives the second driving gear 83 to rotate, the rotating block 7 is rotated, and then the fixed sampling tube 3 is driven to rotate and centrifuge.
In the application, the lower end of the rotating block 7 is provided with an adsorption channel 9 connected in a rotating mode, the adsorption channel 9 is provided with an interface 90, the interface 90 is communicated with a negative pressure chamber through a pipeline, when the device is used, the negative pressure chamber generates negative pressure on the adsorption channel 9 through the pipeline, the adsorption channel 9 generates negative pressure on the adsorption hole 70 of the rotating block 7 through the adsorption channel 9, the adsorption hole 70 of the rotating block 7 adsorbs the bottom end of the fixed sampling tube 3, and the bottom end of the fixed sampling tube 3 is adsorbed and fixed on the rotating block 7.
In some embodiments, the upper end of the sealed bin 2 is provided with a sealing cover 4, the sealing cover 4 is clamped and covered on the upper end of the sealed bin 2, and the sampling tube 3 is positioned in the sealing range of the sealing cover 4; the sealing cover 4 is used for sealing the clamping cover at the upper end of the fixed sampling tube 3, so that the upper end of the fixed sampling tube 3 is positioned in a closed space, and the phage pollution in the fixed sampling tube 3 is reduced. In this application, be provided with the solenoid valve that control breather pipe 5 carried out the break-make on breather pipe 5, during the use, utilize the solenoid valve to control the pipeline break-make of breather pipe 5, if need let in gas to sealed storehouse 2, can carry out the solenoid valve that corresponds and open, carry out sealed storehouse 2 and ventilate.
In the application, a mounting groove 20 is formed in the side wall of the sealed bin 2, a telescopic rod 21 is arranged in the mounting groove 20, the bottom end of the telescopic rod 21 is fixedly mounted at the bottom end of the mounting groove 20, a limiting block 22 is arranged on an upper end rod of the telescopic rod 21, and a spring 23 is sleeved on the telescopic rod 21 at the lower end of the limiting block 22;
the lower end of the sealing cover 4 is provided with a mounting hole, and an induction block is arranged in the mounting hole;
when the sealing cover 4 is clamped in the sealing bin 2, the upper end of the telescopic rod 21 extends into the mounting hole until the upper end of the telescopic rod is abutted against the induction block, the induction block induces a collision signal to the telescopic rod 21 and transmits the collision signal to the controller, and the controller controls the electromagnetic valve to be opened and conducts ventilation and heat preservation on the corresponding sealing bin 2; similarly, when the upper end of the telescopic rod 21 is separated from the induction block, the controller controls the electromagnetic valve to be closed.
In this application, sealed 2 upper ends are provided with seal ring piece 221, and when deciding sampling tube 3 and place in sealed 2, seal ring piece 221 is to being in sealed 2 and deciding the upper end clearance between sampling tube 3 and shielding and closing for deciding sampling tube 3 lower extreme and being in for sealed 2.
Regarding the exhaust of sealed storehouse 2, in this application, seted up first exhaust hole 220 on the seal ring piece 221, sealed lid 4 has seted up second exhaust hole 400, and when sealed lid 4 card lid was in sealed storehouse 2, first exhaust hole 220 and second exhaust hole 400 intercommunication, the gas in sealed storehouse 2 was discharged through first exhaust hole 220 and second exhaust hole 400, avoided sealed storehouse 2 atmospheric pressure too big.
For above telescopic link 21 basis on, when telescopic link 21 stretched into to the mounting hole in, first exhaust hole 220 aligns with second exhaust hole 400, first exhaust hole 220 and second exhaust hole 400 intercommunication.
In the description of the present specification, reference to the description of "one embodiment," "an example," "a specific example," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present disclosure. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The foregoing illustrates and describes the general principles, principal features, and advantages of the present disclosure. It will be understood by those skilled in the art that the present disclosure is not limited to the embodiments described above, which are presented solely for purposes of illustrating the principles of the disclosure, and that various changes and modifications may be made to the disclosure without departing from the spirit and scope of the disclosure, which is intended to be covered by the claims.
Claims (9)
1. A phage separation device for phage culture comprises a support plate (1) and is characterized in that a sealing bin (2) is arranged on the support plate (1), the sealing bin (2) is columnar, and the upper end of the sealing bin (2) is open;
the sealing bins (2) are arranged in a plurality;
a fixed sampling tube (3) is arranged in the sealed bin (2);
one end of the sealed bin (2) is provided with a vent pipe (5), and the vent pipe (5) is connected with an air chamber (6);
the bottom of the sealed bin (2) is provided with a rotating block (7) rotating around the axis of the rotating block, and the rotating block (7) is provided with a plurality of adsorption holes (70).
2. The phage separation apparatus for phage culture according to claim 1, wherein the rotating blocks (7) are coaxially and fixedly installed on a first driving gear (81), the first driving gear (81) is rotatably installed on the support plate (1), the first driving gears (81) among the rotating blocks (7) are connected through a synchronous belt (82), a second driving gear (83) is arranged on the first driving gear (81), a third driving gear (84) is meshed with one side of the second driving gear (83), and the third driving gear (84) is fixedly connected with an output shaft of a motor (85).
3. The phage separation apparatus for phage culture according to claim 1, characterized in that the lower end of the rotating block (7) is provided with an adsorption channel (9) which is rotatably connected, the adsorption channel (9) is provided with a port (90), and the port (90) is communicated with the negative pressure chamber through a pipeline.
4. The phage separation apparatus for phage culture according to claim 1, characterized in that, the sealed storehouse (2) upper end is provided with sealed lid (4), sealed lid (4) card lid is in sealed storehouse (2) upper end.
5. The phage separation apparatus for phage culture according to claim 1, wherein the vent pipe (5) is provided with an electromagnetic valve for controlling the on/off of the vent pipe (5).
6. The phage separation device for phage culture according to claim 5, characterized in that the side wall of the sealed bin (2) is provided with an installation groove (20), an expansion link (21) is arranged in the installation groove (20), the bottom end of the expansion link (21) is fixedly installed at the bottom end of the installation groove (20), the upper end rod of the expansion link (21) is provided with a limit block (22), and the expansion link (21) at the lower end of the limit block (22) is sleeved with a spring (23);
the lower end of the sealing cover (4) is provided with a mounting hole, and an induction block is arranged in the mounting hole;
when sealed lid (4) card lid was in sealed storehouse (2), telescopic link (21) upper end stretched into to the mounting hole in, until inconsistent with the response piece, the conflict signal of response piece response to telescopic link (21), will conflict signal transmission to controller, the controller carries out control solenoid valve.
7. The phage separation apparatus for phage culture according to claim 6, characterized in that, the upper end of the sealing chamber (2) is provided with a sealing ring block (221).
8. The phage separation apparatus for phage culture according to claim 7, wherein the sealing ring block (221) is provided with a first exhaust hole (220), the sealing cover (4) is provided with a second exhaust hole (400), and when the sealing cover (4) is clamped and covered in the sealing bin (2), the first exhaust hole (220) is communicated with the second exhaust hole (400).
9. The phage separation apparatus for phage culture according to claim 7, wherein, when the telescopic rod (21) is extended into the mounting hole, the first exhaust hole (220) is aligned with the second exhaust hole (400).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220516687.4U CN217516948U (en) | 2022-03-10 | 2022-03-10 | Phage separation device for phage culture |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220516687.4U CN217516948U (en) | 2022-03-10 | 2022-03-10 | Phage separation device for phage culture |
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| Publication Number | Publication Date |
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| CN217516948U true CN217516948U (en) | 2022-09-30 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202220516687.4U Active CN217516948U (en) | 2022-03-10 | 2022-03-10 | Phage separation device for phage culture |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116855374A (en) * | 2023-07-24 | 2023-10-10 | 中国水产科学研究院南海水产研究所 | Phage intelligent culture device and control method thereof |
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2022
- 2022-03-10 CN CN202220516687.4U patent/CN217516948U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116855374A (en) * | 2023-07-24 | 2023-10-10 | 中国水产科学研究院南海水产研究所 | Phage intelligent culture device and control method thereof |
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