CN217506262U - Portal frame fluorescence microscope for observing conscious mouse - Google Patents
Portal frame fluorescence microscope for observing conscious mouse Download PDFInfo
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- CN217506262U CN217506262U CN202221293528.9U CN202221293528U CN217506262U CN 217506262 U CN217506262 U CN 217506262U CN 202221293528 U CN202221293528 U CN 202221293528U CN 217506262 U CN217506262 U CN 217506262U
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Abstract
The utility model relates to a portal frame fluorescence microscope for observing conscious mouse. The fluorescence imaging microscope is installed on a top cross beam of the open portal frame through a connecting rod, the objective table is arranged below an objective lens of the fluorescence imaging microscope, the objective table is an air floating cage unit fixed on an XYZ displacement table, the air floating cage unit comprises an air floating platform, a carbon fiber squirrel cage and an air pump, the air pump uniformly provides stable air flow on the surface of the air floating platform, the carbon fiber squirrel cage floats on the air floating platform, a head fixing frame used for fixing the head of a mouse is arranged on the air floating platform, and the head fixing frame is located above the carbon fiber squirrel cage. The utility model discloses a fluorescence microscopic imaging technique carries out the quick fluorescence imaging of multichannel to live body sober mouse to can develop behavioural data record analysis in step.
Description
Technical Field
The utility model belongs to the technical field of microscopic imaging, in particular to a portal frame fluorescence microscope for observing a conscious mouse.
Background
With the popularization and application of optical imaging technology for living animals at home and abroad, more and more researchers hope to observe the growth of tumor cells in the living animals and the reaction of tumor cells to drug treatment through the technology, so that the distribution and metabolic conditions of fluorescently-labeled polypeptides, antibodies and small-molecule drugs in the living animals need to be observed.
In the prior art, the living animal is generally a mouse, and the mouse needs to be anesthetized before head fixation during observation, so that the defects that the activity of cranial nerves is influenced by anesthesia, and the anesthetized mouse cannot be observed in a behavior way. However, if observation is not performed directly without anesthesia, the mouse frequently attempts to move the body, and the stage for holding the mouse is usually fixed with respect to the microscope, and it is difficult to stably observe the mouse when the mouse is forcibly moved.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that a portal frame fluorescence microscope for observing conscious mouse is provided, does not cause the influence to the observation when guaranteeing that the conscious mouse health of no anesthesia removes.
The utility model provides a technical scheme that its technical problem adopted is: the utility model provides a portal frame fluorescence microscope for observing conscious mouse, includes fluorescence imaging microscope and objective table, its characterized in that, fluorescence imaging microscope passes through the connecting rod and installs on open portal frame's top crossbeam, the crossbeam can move in X and Y direction, fluorescence imaging microscope's objective below is located to the objective table, the objective table is for fixing the air supporting cage unit on XYZ displacement platform, air supporting cage unit includes air supporting platform, carbon fiber squirrel cage and air pump, the air pump is in air supporting platform provides steady air current on the surface uniformly, makes the carbon fiber squirrel cage floats on the air supporting platform, be equipped with the head mount that is used for fixed mouse head on the air supporting platform, the head mount is located the top of carbon fiber squirrel cage.
Furthermore, a guide rail and a ball screw are arranged on the beam of the open portal frame in the X and Y directions, two turntables which are respectively used for controlling the beam to move in the XY direction are arranged on the ball screw, and a screw device which can control the fluorescence imaging microscope to move in the Z direction is arranged on the connecting rod.
Furthermore, magnetic force electrodes are paved at the bottom of the carbon fiber mouse cage, and a position sensor used for sensing and recording position changes of the magnetic force electrodes is arranged in the air floatation platform.
Furthermore, a CMOS camera is arranged on the fluorescence imaging microscope.
Furthermore, a multi-wavelength LED fluorescent light source is arranged on one side of the fluorescence imaging microscope, and a multi-pass fluorescence excitation block unit is arranged inside the fluorescence imaging microscope.
Furthermore, the multi-pass fluorescence excitation block unit comprises a multi-pass excitation filter, a multi-pass dichroic mirror and a multi-pass emission filter.
Advantageous effects
The utility model discloses a fixed objective table with conventionality improves to an air supporting platform, to the fixed back of conscious mouse head, makes the activity of conscious mouse health concentrate on the air supporting platform, therefore can carry out stable formation of image to the head to this guarantees whole real neural network formation of image and record.
The utility model discloses a still be equipped with position sensor in the air supporting platform, can take notes the position change of magnetic force electrode for position sensor to through the removal orbit data of the visual clear-headed mouse health that demonstrate of software, be convenient for carry out further data analysis.
The utility model discloses an establish microscope stand on open portal frame, for the removal of air supporting platform provides great space, level and high demand when satisfying to erect and operating the electrode a little, the shake problem when further reducing optical imaging simultaneously.
Drawings
FIG. 1 is a perspective view of a gantry fluorescence microscope used to observe awake mice.
FIG. 2 is a schematic diagram of a gantry fluorescence microscope used to observe conscious mice.
Wherein, 1-multi-pass fluorescence excitation block unit; 2-an air floating cage unit; 201-an air floating platform; 202-carbon fiber squirrel cage; 203-trachea; 204-head mount; 205-a magnetic electrode; 206-a position sensor; 3-XYZ displacement stage; 301-a knob; 302-differential head; 4-open gantry; 401-a beam; 402-a turntable; 403-a connecting rod; 5-CMOS camera; 6-a multi-wavelength LED fluorescent light source; 7-fluorescence imaging microscope; 701-fluorescent turntable.
Like reference symbols in the various drawings indicate like elements.
Detailed Description
The present invention will be further described with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Furthermore, it should be understood that various changes and modifications of the present invention may be made by those skilled in the art after reading the teachings of the present invention, and these equivalents also fall within the scope of the appended claims.
As shown in fig. 1 and 2, the utility model provides a portal frame fluorescence microscope for observing a conscious mouse, which comprises a fluorescence imaging microscope 7 and an objective table. The fluorescence imaging microscope 7 is mounted on a top beam 401 of the open gantry 4 through a connecting rod 403, and the beam 401 can move in the X and Y directions. The objective table is arranged below an objective lens of the fluorescence imaging microscope 7, the objective table is an air floating cage unit 2 fixed on an XYZ displacement table 3, and the XYZ displacement table 3 can move in three XYZ directions and drive the air floating cage unit 2 to move correspondingly under the control of a knob 301 and a differential head 302. The connecting rod 403 is a 32mm stainless steel rod, and a screw device is arranged on the connecting rod, so that the fluorescence imaging microscope 7 can move in the Z-axis direction relative to the beam 401.
The air floating cage unit 2 comprises an air floating platform 201, a carbon fiber squirrel cage 202 and an air pump. The air pump is connected with the air floating platform 201 through an air pipe 203, small holes are arranged on the air floating platform 201 in an array mode, air input by the air pump uniformly provides stable air flow on the surface of the air floating platform 201 through the small holes, the carbon fiber mouse cage 202 floats on the air floating platform 201, a head fixing frame 204 used for fixing the head of a mouse is arranged on the air floating platform 201, and the head fixing frame 204 is located above the carbon fiber mouse cage 202.
The carbon fiber mouse cage 202 is of a culture dish type and is placed in the center of the air floating platform 201 in an initial state. The area of the air floatation platform 201 is far larger than that of the carbon fiber mouse cage 202, so that the carbon fiber mouse cage 202 has sufficient moving space when the body of a mouse positioned at the center moves.
Further, the objective lens magnification of the fluorescence imaging microscope 7 is adjustable.
Further, the head holder 204 is used for fixing the mouse after the surgical craniotomy, a titanium alloy head plate and the mouse skull can be adhered together through dental cement, and the titanium alloy head plate can be fixedly connected with the head holder 204 through bolts, clamping pins or other modes, so that the head of the mouse is prevented from shaking during observation.
Further, the open type portal frame 4 is fixed on the optical platform, the four-leg bracket of the open type portal frame 4 is composed of 4 aluminum alloy sections 50x50mm, a fixing plate is arranged at the bottom of each aluminum alloy section, and the fixing plate is fixed on the optical platform through bolts.
Further, a guide rail and a ball screw are arranged on the beam 401 of the open portal frame 4 in the X and Y directions, two turntables 402 which are respectively used for controlling the beam 401 to move in the XY directions are arranged on the ball screw, the ball screw can be controlled to drive the beam 401 to move by rotating the turntables 402, and the corresponding objective lens of the fluorescence imaging microscope 7 can also move until the objective lens is aligned with a region to be observed.
Further, magnetic force electrodes 205 are paved at the bottom of the carbon fiber mouse cage 202, and a position sensor 206 for sensing and recording position changes of the magnetic force electrodes 205 is arranged in the air floating platform 201. The magnetic electrode 205 can be flatly laid on the whole bottom of the carbon fiber squirrel cage 202 or arranged in the center of the bottom of the carbon fiber squirrel cage 202, and the position sensor 206 can be arranged at any position in the air floating platform 201 according to conditions, and is preferably arranged below an air path connected with an air pump in the air floating platform 201. The position sensor 206 may be linked with an external device in a wired or wireless manner, and may transmit data through a program.
Further, a CMOS camera 5 is disposed on the fluorescence imaging microscope 7. After the fluorescence image is collected by the objective lens of the fluorescence imaging microscope 7, the fluorescence image is focused on the target surface of the CMOS camera 5 through the imaging lens to perform dynamic imaging recording. The CMOS camera 5 may be linked with an external device in a wired or wireless manner, and controls and transmits data through a preset linkage program.
Furthermore, a multi-wavelength LED fluorescent light source (6) is arranged on one side of the fluorescence imaging microscope (7), and the multi-wavelength LED fluorescent light source 6 provides an excitation light source for the fluorescence imaging microscope 7 through a multi-channel fluorescence excitation block unit 1 arranged in the fluorescence imaging microscope 7. The wavelength of the light source provided by the LED fluorescent light source is adjustable, the number of selectable wavelengths is at least more than 2, and the irradiation of transgenic mice with different fluorescent markers can be met. The multi-wavelength light source is used for simultaneously exciting and imaging two or more fluorescent labels, and can be used for experimental means such as fluorescence ratio analysis. The multi-wavelength LED fluorescent light source 6 can be linked with external equipment and the CMOS camera 5 in a wired or wireless mode and is controlled through a preset linkage program.
Further, the multi-pass fluorescence excitation block unit 1 is disposed in a fluorescence turntable 701 on one side of the fluorescence imaging microscope 7, and includes a multi-pass excitation filter, a multi-pass dichroic mirror, and a multi-pass emission filter, and switches and filters the multi-wavelength light beam provided by the multi-wavelength LED fluorescence light source 6 by rotating the fluorescence turntable 701.
The utility model discloses a fluorescence microscopic imaging technique carries out the quick fluorescence imaging of multichannel to live body sober mouse to can develop behavioural data record analysis in step. The high-power multi-wavelength LED fluorescent light source 6 is used for irradiating, the light emitting condition of the intracranial fluorescence marker of the transgenic mouse is observed, the data such as the growth of tumor cells and the reaction condition of the drug are accurately obtained, and the qualitative and drug property analysis of key reaction factors is realized.
In the experiment, the transgenic mice are labeled with fluorescence in the intracranial space in advance, and the fluorescent substance in the tissue or blood vessel is released after the irradiation of the excitation light, so that the observation can be performed by the fluorescence imaging microscope 7. Fixing the head of a conscious mouse under the head fixing frame 204, and sending the air floating platform 201 to the lower part of the fluorescence imaging microscope 7 by adjusting the XYZ displacement table 3; finely adjusting the position of the portal frame to focus the 1.25-time objective lens of the fluorescence imaging microscope 7 and clearly find a brain area to be observed; switching the objective lens to a 10-time objective lens to continuously focus and observe details of the brain tissue sample; the wavelength and intensity of the excitation light are set by a control panel of the multi-wavelength LED fluorescent light source 6, and multi-wavelength fluorescence imaging collection is carried out by using an external control program.
When the mouse is observed, the body of the mouse is stimulated to continuously try to move, so that the carbon fiber mouse cage 202 moves relative to the air floating platform 201. While keeping the head fixed and not influencing microscopic observation, the position sensor 206 can sense and record the displacement of the magnetic electrode 205 on the carbon fiber squirrel cage 202, and the moving track data of the mouse can be displayed visually through software. Finally, the optical imaging data and the track data can be synchronously analyzed.
Claims (6)
1. A portal frame fluorescence microscope for observing a conscious mouse comprises a fluorescence imaging microscope (7) and an object stage, and is characterized in that the fluorescence imaging microscope (7) is mounted on a top cross beam (401) of an open portal frame (4) through a connecting rod (403), the cross beam (401) can move in the X and Y directions, the object stage is arranged below an objective lens of the fluorescence imaging microscope (7), the object stage is an air floating cage unit (2) fixed on an XYZ displacement table (3), the air floating cage unit (2) comprises an air floating platform (201), a carbon fiber mouse cage (202) and an air pump, the air pump uniformly provides stable air flow on the surface of the air floating platform (201), so that the carbon fiber mouse cage (202) floats on the air floating platform (201), the air floating platform (201) is provided with a head fixing frame (204) for fixing the head of the mouse, the head mount (204) is located above the carbon fiber squirrel cage (202).
2. A gantry fluorescence microscope for observing awake mice according to claim 1, characterized in that the cross beam (401) of the open gantry (4) is provided with guide rails and ball screws in the X and Y directions, the ball screws are provided with two rotating discs (402) for controlling the movement of the cross beam (401) in the XY directions, respectively, and the connecting rod (403) is provided with a screw device for controlling the movement of the fluorescence imaging microscope (7) in the Z direction.
3. The gantry fluorescence microscope for observing awake mice according to claim 1, wherein magnetic force electrodes (205) are laid on the bottom of the carbon fiber mouse cage (202), and a position sensor (206) for sensing and recording position changes of the magnetic force electrodes (205) is arranged in the air-floating platform (201).
4. Gantry fluorescence microscope for observing awake mice according to claim 1, characterized in that a CMOS camera (5) is provided on the fluorescence imaging microscope (7).
5. The portal frame fluorescence microscope for observing awake mice according to claim 1, wherein the fluorescence imaging microscope (7) is provided with a multi-wavelength LED fluorescence light source (6) at one side and a multi-pass fluorescence excitation block unit (1) inside.
6. The gantry fluorescence microscope of claim 5, wherein the multi-pass fluorescence excitation block unit (1) comprises a piece of multi-pass excitation filter, a piece of multi-pass dichroic mirror and a piece of multi-pass emission filter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221293528.9U CN217506262U (en) | 2022-05-27 | 2022-05-27 | Portal frame fluorescence microscope for observing conscious mouse |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221293528.9U CN217506262U (en) | 2022-05-27 | 2022-05-27 | Portal frame fluorescence microscope for observing conscious mouse |
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| Publication Number | Publication Date |
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| CN217506262U true CN217506262U (en) | 2022-09-27 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202221293528.9U Active CN217506262U (en) | 2022-05-27 | 2022-05-27 | Portal frame fluorescence microscope for observing conscious mouse |
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2022
- 2022-05-27 CN CN202221293528.9U patent/CN217506262U/en active Active
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