CN217490430U - Circulating collection bottle for tangential flow ultrafiltration concentration - Google Patents
Circulating collection bottle for tangential flow ultrafiltration concentration Download PDFInfo
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- CN217490430U CN217490430U CN202221212063.XU CN202221212063U CN217490430U CN 217490430 U CN217490430 U CN 217490430U CN 202221212063 U CN202221212063 U CN 202221212063U CN 217490430 U CN217490430 U CN 217490430U
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Abstract
The utility model provides a circulating collecting bottle for tangential flow ultrafiltration concentration, which comprises a bottle body and a heat-insulating sleeve; the top of the bottle body is constricted to form a bottle mouth, the bottle mouth is provided with a bottle cap, the upper part of the bottle body is provided with a liquid inlet and a liquid outlet, and the liquid outlet is connected with a liquid outlet pipe arranged in the bottle; the heat-insulating sleeve wraps the middle lower part of the bottle body, is hollow and is filled with a refrigerant. The utility model discloses a circulation receiving flask simple structure, convenient to use have solved the problem of mixed fungus pollution, liquid loss and active substance loss in the tangential flow ultrafiltration liquid collecting process, are applicable to the laboratory and the production process of biological sample clinically.
Description
Technical Field
The utility model relates to a test instrument, concretely relates to circulation receiving flask that is used for concentrated system of tangential flow ultrafiltration.
Background
Tangential flow ultrafiltration (TFF) is a form of filtration in which the direction of liquid flow is perpendicular to the direction of filtration, and is mainly characterized by avoiding rapid increase of filtration resistance and ensuring stable filtration speed, thereby achieving higher membrane filtration throughput and enabling rapid and efficient separation and purification of biomolecules.
The tangential flow ultrafiltration system mainly comprises a peristaltic pump, a hollow fiber column, a liquid inlet pipe, a waste liquid pipe and a backflow pipe, wherein the liquid inlet pipe, the waste liquid pipe and the backflow pipe are directly placed into a container such as a wide-mouth bottle or a beaker in the current experimental operation and are fixed at a bottle mouth or not fixed by a clamp. Because the tangential flow system can produce vibrations at work, feed liquor pipe, waste liquid pipe, back flow roll-off bottleneck easily, contact each other easily between the pipeline simultaneously, increase the pollution risk of sample, be unfavorable for the collection of sample cell and the development of follow-up experiment. Meanwhile, since the tangential flow system has a long working time and is carried out at normal temperature, functional substances in cell supernatant, such as extracellular vesicles and cytokines, may be affected, and further application of the concentrated supernatant is not facilitated.
Disclosure of Invention
To the problem that lacks stable, heat retaining tangential flow ultrafiltration receiving flask at present, the utility model provides a circulation receiving flask for tangential flow ultrafiltration is concentrated, simple structure, convenient to use.
In order to achieve the above purpose, the utility model adopts the following technical scheme.
A circulation collecting bottle for tangential flow ultrafiltration concentration comprises a bottle body and a heat-insulating sleeve;
the top of the bottle body is constricted to form a bottle mouth, the bottle mouth is provided with a bottle cap, the upper part of the bottle body is provided with a liquid inlet and a liquid outlet, the liquid outlet is connected with a liquid outlet pipe arranged in the bottle, and the liquid inlet and the liquid outlet are both provided with dust caps;
the upper part of the heat-insulating sleeve is provided with an opening and wraps the middle lower part of the bottle body, and the heat-insulating sleeve is hollow and filled with a refrigerant.
Preferably, the bottle cap is a double-layer cap, the outer cap is a sealing cap, and the inner cap is provided with a waterproof breathable film.
Preferably, the bottle body is provided with a scale line for displaying the volume.
More preferably, the heat preservation sleeve is provided with a scale display area; the scale display area is an incised or transparent area.
Preferably, the liquid inlet and the liquid outlet form an included angle of 60 degrees with the bottom surface of the bottle body.
When the circulation collecting bottle for tangential flow ultrafiltration concentration is used, the circulation collecting bottle is connected with each pipeline of tangential flow ultrafiltration, a liquid outlet is connected with a liquid inlet pipe in a tangential flow system, a liquid inlet is connected with a return pipe in the tangential flow system, a bottle body is inserted into a pre-frozen heat-insulating sleeve, a bottle opening is ventilated, and an ultrafiltration pump can be started to work. The liquid volume in the bottle body can be observed through the scale of the bottle body, and the concentration degree of the tangential flow ultrafiltration is controlled.
The utility model has the advantages of it is following:
the utility model provides a circulation receiving flask for tangential flow ultrafiltration is concentrated sets up inlet and liquid outlet and is used for connecting ultrafiltration system's feed liquor pipe and back flow respectively, has avoided two pipelines all to get into the inside condition of bottle through the bottleneck, can reduce the pipe wall and pollute the concentrate on the one hand, and on the other hand can increase the pipeline stability, prevents that the pipeline from having reduced the loss risk owing to vibrations from the condition of bottleneck landing. In addition, the circulating collection bottle is also provided with a heat insulation sleeve, so that the liquid can be kept at a lower temperature in the ultrafiltration process, and the loss of active substances is reduced. The utility model discloses a circulation receiving flask simple structure, convenient to use have solved the problem of mixed fungus pollution, liquid loss and active substance loss in the tangential flow ultrafiltration liquid collecting process, are applicable to the laboratory and the production process of biological sample clinically.
Drawings
FIG. 1 is a schematic view of the construction of a recycling collection bottle;
FIG. 2 is a schematic structural view of the insulating jacket;
FIG. 3 is a schematic structural view of the thermal jacket according to example 1;
FIG. 4 is a schematic structural view of the thermal insulating jacket according to embodiment 2;
wherein, 1-bottle body, 2-insulating sleeve, 3-bottle cap, 4-liquid inlet, 5-liquid outlet, 6-liquid outlet pipe, 7-scale mark and 8-scale display area.
Detailed Description
The present invention will be further described with reference to the following examples and drawings, but the present invention is not limited to the following examples.
Example 1
The circulation collecting bottle for tangential flow ultrafiltration concentration as shown in fig. 1-3 comprises a bottle body 1 and a heat insulating sleeve 2;
the top of the bottle body 1 is constricted to form a bottle mouth, the bottle mouth is provided with a bottle cap 3, the bottle cap 3 is a double-layer cap, the outer cap is a sealing cap, and the inner cap is provided with a waterproof breathable film; a liquid inlet 4 and a liquid outlet 5 are arranged at the upper part of the bottle body 1, the liquid outlet 5 is connected with a liquid outlet pipe 6 arranged in the bottle, the liquid inlet 4 and the liquid outlet 5 form an included angle of 60 degrees with the bottom of the bottle body, and both the liquid inlet 4 and the liquid outlet 5 are provided with dust covers; the bottle body 1 is provided with scale marks 7 for displaying the volume;
the upper part of the heat insulation sleeve 2 is provided with an opening, the heat insulation sleeve is wrapped at the middle lower part of the bottle body 1, the interior of the heat insulation sleeve is hollow, and CMC and water-absorbing resin are filled in the heat insulation sleeve to be used as refrigerant storage agents; the heat insulation sleeve 2 is provided with a scale display area 8; the scale display area is a transparent area which is not filled with refrigerant.
When the device is used, liquid is added into the bottle body, the liquid outlet is connected with the liquid inlet pipe in the tangential flow system, the liquid inlet is connected with the return pipe in the tangential flow system, the bottle body is inserted into the pre-frozen heat insulation sleeve, the scale of the bottle body is aligned with the scale display area of the heat insulation sleeve, the outer cover is opened, the inner cover enables the bottle mouth to ventilate, and the ultrafiltration pump can be started to work. The liquid volume in the bottle body can be observed through the scale of the bottle body, the concentration degree of tangential flow ultrafiltration is controlled, and the bottle is started and stopped according to requirements; after the use, the pipeline is detached from the bottle, the dustproof plugs are arranged on the liquid inlet and the liquid outlet, the bottle cap is taken down, and the liquid is poured out.
Example 2
The circulation collecting bottle for tangential flow ultrafiltration concentration as shown in fig. 1-2 and fig. 4 comprises a bottle body 1 and a heat insulating sleeve 2;
the top of the bottle body 1 is constricted to form a bottle mouth, the bottle mouth is provided with a bottle cap 3, the upper part of the bottle body is provided with a liquid inlet 4 and a liquid outlet 5, the liquid outlet 5 is connected with a liquid outlet pipe 6 arranged in the bottle, the liquid inlet 4 and the liquid outlet 5 form an included angle of 60 degrees with the bottom of the bottle body, and the liquid inlet 4 and the liquid outlet 5 are both provided with dust caps; the bottle body 1 is provided with scale marks 7 for displaying the capacity;
the upper part of the heat-insulating sleeve 2 is provided with an opening, the heat-insulating sleeve is wrapped at about 1/2 parts of the middle lower part of the bottle body 1, the interior of the heat-insulating sleeve is hollow, and CMC and water-absorbing resin are filled in the heat-insulating sleeve to serve as refrigerant; the heat insulation sleeve 2 is provided with a scale display area 8; the scaled display area is an indentation.
When the device is used, liquid is added into the bottle body, the liquid outlet is connected with the liquid inlet pipe in the tangential flow system, the liquid inlet is connected with the return pipe in the tangential flow system, the bottle body is inserted into the pre-frozen heat insulation sleeve, the scale of the bottle body is aligned with the scale display area of the heat insulation sleeve, the bottle cover is unscrewed to enable the bottle mouth to be ventilated, and the ultrafiltration pump is started to work. The liquid volume in the bottle body can be observed through the scale of the bottle body, the concentration degree of tangential flow ultrafiltration is controlled, and the bottle is started and stopped according to requirements; after use, the pipeline is detached from the bottle, the dustproof plugs are arranged on the liquid inlet and the liquid outlet, the bottle cap is taken down, and liquid is poured out.
Claims (5)
1. A circulation collecting bottle for tangential flow ultrafiltration concentration is characterized by comprising a bottle body and a heat-insulating sleeve; the top of the bottle body is constricted to form a bottle mouth, the bottle mouth is provided with a bottle cap, the upper part of the bottle body is provided with a liquid inlet and a liquid outlet, the liquid outlet is connected with a liquid outlet pipe arranged in the bottle, and the liquid inlet and the liquid outlet are both provided with dust caps; the upper part of the heat-insulating sleeve is provided with an opening and wraps the middle lower part of the bottle body, and the heat-insulating sleeve is hollow and filled with a refrigerant.
2. The recycling collection bottle of claim 1, wherein said cap is a double cap, the outer cap is a sealing cap and the inner cap is provided with a water impermeable, gas permeable membrane.
3. The recycling collection bottle of claim 1, wherein said body is provided with graduations indicating capacity.
4. The recycling collection bottle of claim 1, wherein said boot is provided with a scale display area; the scale display area is an incised or transparent area.
5. The recycling collection bottle of claim 1, wherein the liquid inlet and outlet are angled at 60 ° from the bottom of the bottle body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221212063.XU CN217490430U (en) | 2022-05-20 | 2022-05-20 | Circulating collection bottle for tangential flow ultrafiltration concentration |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221212063.XU CN217490430U (en) | 2022-05-20 | 2022-05-20 | Circulating collection bottle for tangential flow ultrafiltration concentration |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217490430U true CN217490430U (en) | 2022-09-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221212063.XU Active CN217490430U (en) | 2022-05-20 | 2022-05-20 | Circulating collection bottle for tangential flow ultrafiltration concentration |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217490430U (en) |
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2022
- 2022-05-20 CN CN202221212063.XU patent/CN217490430U/en active Active
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|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230814 Address after: 511400 702, Floor 7, Building 8 (Building 9), No. 6, Nanjiang Second Road, the Pearl River Street, Nansha District, Guangzhou, Guangdong Patentee after: Guangdong Yinfeng Cell Technology Co.,Ltd. Patentee after: YINFENG BIOLOGICAL GROUP Co.,Ltd. Address before: 117-B3, Supporting Office Area of Customs Supervision Warehouse, Jinan Comprehensive Bonded Zone, No. 5999, Aerospace Avenue, High-tech Zone, Jinan City, Shandong Province, 250013 (Business location: Room 201, 2nd Floor, Phase 1, Yinfeng Biotechnology Park, Gangyuan 6th Road) Patentee before: QILU CELL THERAPY TECHNOLOGY CO.,LTD. Patentee before: YINFENG BIOLOGICAL GROUP Co.,Ltd. |
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| TR01 | Transfer of patent right |