CN217438166U

CN217438166U - A support device that is used for mouth pipette of unicellular separation

Info

Publication number: CN217438166U
Application number: CN202121322920.7U
Authority: CN
Inventors: 高芳芳
Original assignee: Suzhou Yikang Medical Laboratory Co ltd
Current assignee: Suzhou Yikang Medical Laboratory Co ltd
Priority date: 2021-06-15
Filing date: 2021-06-15
Publication date: 2022-09-16
Anticipated expiration: 2031-06-15

Abstract

In order to solve the problems of inaccurate cell picking and low cell picking efficiency caused by unstable operation when the breach suction pipe picks cells, the utility model provides a bracket device of a breach suction pipe for single cell sorting, so that when the oral suction tube is used for picking cells, part of the capillary tube fixing device of the oral suction tube can be arranged on the bracket, the bracket provides a stress point for the capillary tube device, the capillary tube has better stability when the capillary tube is controlled by hand strength, so that a novice in a laboratory can finish the cell picking work through simple training, and because a stress point is given to the oral suction tube device in the cell picking process, the fatigue of the arm is relieved when the cells are picked for a long time, the cell picking flux is increased, and the work which can be carried out by purchasing expensive cell separation equipment can be finished by picking the cells through the simple oral suction tube.

Description

A support device that is used for mouth pipette of unicellular separation

Technical Field

The utility model relates to a laboratory appurtenance technical field, it is comparatively concrete, involve a support device that is used for unicellular oral siphon of selecting separately.

Background

The cell is the basic unit of biology, and along with the development of technology, the research to the organism has entered into unicellular research stage, and for unicellular research, the first stage of research is the separation of unicellular, the utility model relates to the improvement of unicellular separation instrument equipment in the unicellular separation process.

In cell-related experiments, it is often necessary to sort cells from a population into individual cells in order to study the function of a single cell, or to perform single cell sequencing. In the aspect of single cell sorting, a micromanipulator, a flow cell sorter, a micropore chip technology, a microfluidic technology and the like can sort the group cells into single cells. However, these cell sorting instruments are expensive, and their consumables are very expensive, and most researchers do not have sufficient financial resources to purchase such large-scale equipment. Therefore, it is desirable to design an oral pipette device for single cell sorting.

The prior oral suction tube can be divided into 5 components after being assembled, and as shown in figure 1, the assembly process of the oral suction tube is as follows: inserting a clean gun head with a filter element into a part of gun head fixing device, inserting the thicker part of the tail end of the prepared capillary tube into the cavity of the capillary tube fixing device at the other end part, wherein the tail end extends into the cavity by 1mm, and the two ends are connected by a part of rubber hoses.

The operator keeps observing the microscope posture, holds partial capillary fixing device with the right hand, and holds the microscope objective table with the left hand to prevent the visual field from drifting. And adjusting the microscope to a low power lens, repeatedly scanning the tip of the capillary tube in the field of view of the microscope, determining the position of the needle tip, and keeping the needle tip above the liquid level without contacting the liquid. And (5) after finding the needle point position, keeping the right hand still, and adjusting the magnification of the microscope to the required high power lens. The needle point keeps the blowing state before entering the liquid level, and then the tongue tip is used for blocking the gun head to ensure that no liquid siphons into the needle head and no air bubbles are generated after the capillary tube enters the liquid level. Slowly moving the needle to the position near the target cell, loosening the tongue tip blocking the gun head, sucking the cell into the needle head under the siphon action, and quickly lifting the needle away from the liquid level after success. The volume of liquid entering the capillary does not exceed the finer part of the capillary (about 1. mu.L). When cells are picked up using the mouth pipette, the operation is performed by observing under a 100 × (eyepiece 10 ×, objective lens 10 ×) mirror, and the fine hand shaking during the operation is extremely remarkable when the microscope is under a microscope, and particularly, the hand shaking becomes more remarkable for beginners and a large number of cells to be picked up.

When the capillary is close to the cell from the upper part of the liquid surface, the hand is in a suspension state because the hand has no fulcrum, and the instability of the hand inevitably occurs, thereby causing the change of the position of the cell or the cell exceeding the visual field range when the capillary is close to the liquid surface. Sometimes, the capillary tube may be severely scratched on the surface of the culture dish due to instability of the hand, which may affect the observation.

Due to the above problems, the conventional oral pipette device has a high requirement for the skill of the operator, and even when the operator picks up cells using the conventional oral pipette, the operator still has problems that the picked cells are inaccurate and the picking efficiency is low.

SUMMERY OF THE UTILITY MODEL

In view of the above, in order to solve the problems of inaccurate cell picking and low cell picking efficiency caused by unstable operation when the oral pipette picks cells, the utility model provides a bracket device of the oral pipette for single cell sorting, so that when the oral suction tube is used for picking cells, part of the capillary tube fixing device of the oral suction tube can be arranged on the bracket, the bracket provides a stress point for the capillary tube device, the capillary tube has better stability when the capillary tube is controlled by hand strength, so that a novice in a laboratory can finish the cell picking work through simple training, and because a stress point is given to the oral suction tube device in the cell picking process, the fatigue of the arm is relieved when the cells are picked for a long time, the cell picking flux is increased, and the work which can be carried out by purchasing expensive cell separation equipment can be finished by picking the cells through the simple oral suction tube.

A support device of a mouth suction pipe for single cell sorting comprises a mouth suction pipe and a support device, wherein a capillary fixing device 3 is arranged on the mouth suction pipe, and the capillary fixing device 3 is positioned on the support device.

Further, the bracket 6 has an arc-shaped structure so as to be coupled with the capillary tube fixing device 3.

Furthermore, the connecting rod 7 is a telescopic rod, and the height of the connecting rod can be adjusted, so that the supporting bracket device can be used when the lengths of the capillary tubes 4 are different.

Further, the bracket 6 is rotatably connected with one end of the connecting rod 7, and the bracket 6 can rotate at any angle.

Further, the capillary tube fixing means 3 of the oral pipette is slidably detachably connected to the bracket 6 to increase the flexibility of the capillary tube 4.

Further, the connecting rod 7 is vertically connected to the center of the bracket 6.

Further, fixing base 8 is for falling the loudspeaker structure, and 8 upper portions of fixing base are equipped with the connecting block, and the connecting block is cylindrical.

Further, the connecting rod 7 is vertically connected with the center of the bracket 6, and the connecting rod 7 is vertically connected with the center of the upper end of the connecting block, so that the connecting rod 7, the center of the bracket 6 and the center of the fixing seat 8 are in a straight line.

Further, the radian of the arc-shaped structure is larger than the diameter of the capillary tube fixing device 3 of the oral suction tube.

Further, the support device is made of stainless steel or plastic.

The utility model has the advantages that: the utility model provides a support device for oral siphon of unicellular separation, so that when using oral siphon to choose the cell, make partial capillary fixing device 3 of oral siphon can arrange in on the support, give capillary 4 device stress point by the support, capillary 4 stability is better when using hand strength control capillary 4, make the novice in laboratory can accomplish the cell through simple training and choose the work, and because choose the in-process for oral siphon device stress point at the cell, the tired sense of arm when having alleviateed long-time cell and choosing, the cell flux of choosing has been increased, the work that need purchase expensive cell separation equipment just can develop before the messenger can be accomplished through the cell of choosing through simple oral siphon.

Drawings

FIG. 1 is a view showing the structure of the mouth suction pipe of the holder device for mouth suction pipe for single cell sorting of the present invention.

Fig. 2 is an overall schematic view of the rack device of the oral pipette for single cell sorting of the present invention.

Description of the main elements

The following detailed description of the invention will be further described in conjunction with the above-identified drawings.

Detailed Description

The following examples are described to aid in the understanding of the present application and are not, and should not be construed to, limit the scope of the present application in any way.

In the following description, those skilled in the art will recognize that components may be described throughout this discussion as separate functional units (which may include sub-units), but those skilled in the art will recognize that various components or portions thereof may be divided into separate components or may be integrated together (including being integrated within a single system or component).

Also, connections between components or systems within the figures are not intended to be limited to direct connections. Rather, data between these components may be modified, reformatted, or otherwise changed by the intermediary components. Additionally, additional or fewer connections may be used. It should also be noted that the terms "coupled," "connected," or "input" and "fixed" should be understood to encompass direct connections, indirect connections, or fixed through one or more intermediaries.

In the description of the present application, it should be noted that the terms "center", "upper", "lower", "left", "right", "side", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on orientations or positional relationships shown in the drawings or orientations or positional relationships commonly recognized in the product of the application, and are only used for convenience in describing the present application and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present application. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance. Furthermore, the terms "horizontal", "vertical" and the like do not imply that the components are required to be absolutely horizontal or pendant, but rather may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.

The embodiment is as follows:

as shown in FIG. 1, it is a structural diagram of the oral pipette of the bracket device of the oral pipette for single cell sorting of the present invention; as shown in FIG. 2, the overall schematic diagram of the rack device of the oral pipette for single cell sorting of the present invention is shown.

The bracket 6 is of an arc structure so as to be matched and connected with the capillary tube fixing device 3.

The connecting rod 7 is a telescopic rod and can be used for adjusting the height of the device, so that the supporting bracket devices can be used when the lengths of the capillary tubes 4 are different.

The bracket 6 is rotatably connected with one end of the connecting rod 7, and the bracket 6 can rotate at any angle.

The capillary tube holder 3 of the pipette is slidably removably attached to the carriage 6 to increase the flexibility of the capillary tube 4.

The connecting rod 7 is vertically connected to the center of the bracket 6.

Fixing base 8 is for falling the loudspeaker structure, and 8 upper portions of fixing base are equipped with the connecting block, and the connecting block is cylindrical.

The connecting rod 7 is vertically connected with the center of the bracket 6, and the connecting rod 7 is vertically connected with the center of the upper end part of the connecting block, so that the connecting rod 7, the center of the bracket 6 and the center of the fixed seat 8 are on the same straight line.

The radian of the arc-shaped structure is larger than the diameter of the capillary tube fixing device 3 of the oral suction tube.

The support device is made of stainless steel or plastic.

The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims

1. A support device of a mouth suction pipe for single cell sorting comprises a mouth suction pipe and a support device, wherein a capillary fixing device (3) is arranged on the mouth suction pipe, the capillary fixing device (3) is positioned on the support device, and the support device is characterized by comprising a bracket (6), a connecting rod (7) and a fixing seat (8), one end of the connecting rod (7) is connected with the bracket (6), the other end of the connecting rod (7) is connected with the fixing seat (8), and the capillary fixing device (3) is positioned on the bracket (6).

2. The rack device of oral pipette for single cell sorting of claim 1, wherein: the bracket (6) is of an arc-shaped structure so as to be conveniently matched and connected with the capillary tube fixing device (3).

3. The rack device of oral pipette for single cell sorting of claim 1, wherein: the connecting rod (7) is a telescopic rod and a device capable of adjusting the height, so that the supporting bracket device can be used when the lengths of the capillary tubes (4) are different.

4. The rack device of oral pipette for single cell sorting of claim 1, wherein: the bracket (6) is rotatably connected with one end of the connecting rod (7), and the bracket (6) can rotate at any angle.

5. The rack device of oral pipette for single cell sorting of claim 1, wherein: the capillary tube fixing device (3) of the oral suction tube is connected with the bracket (6) in a sliding and detachable mode so as to increase the flexibility of the capillary tube (4).

6. The rack device of oral pipette for single cell sorting of claim 1, wherein: the connecting rod (7) is vertically connected with the center of the bracket (6).

7. The rack device of oral pipette for single cell sorting of claim 1, wherein: the fixing seat (8) is of an inverted horn structure, the upper portion of the fixing seat (8) is provided with a connecting block, and the connecting block is cylindrical.

8. The holder device for a mouth pipette for single cell sorting of claim 7, wherein: the connecting rod (7) is vertically connected with the center of the bracket (6), and the connecting rod (7) is vertically connected with the center of the upper end part of the connecting block, so that the connecting rod (7), the center of the bracket (6) and the center of the fixed seat (8) are on the same straight line.

9. The rack device of oral pipette for single cell sorting of claim 1, wherein: the radian of the arc-shaped structure is larger than the diameter of the capillary tube fixing device (3) of the oral suction tube.

10. The rack device of oral pipette for single cell sorting of claim 1, wherein: the support device is made of stainless steel or plastic.