CN217438162U

CN217438162U - Device for capturing biomolecules, cells or bacteria

Info

Publication number: CN217438162U
Application number: CN202221363281.3U
Authority: CN
Inventors: 颜菁; 程林; 邹长华
Original assignee: Shanghai Bangxian Medical Technology Co ltd
Current assignee: Shanghai Bangxian Medical Technology Co ltd
Priority date: 2022-05-24
Filing date: 2022-05-24
Publication date: 2022-09-16
Anticipated expiration: 2032-05-24

Abstract

The utility model discloses a capture device of biomolecule, cell or bacterium, including anchor clamps, be formed with the passageway that supplies the sample to flow through in the anchor clamps, capture device still including set up in first capture unit and second capture unit in the anchor clamps, first capture unit is including being used for the filtering first screen cloth of impurity in the sample, the second capture unit including have be used for with the second screen cloth that biomolecule, cell or bacterium specificity combined, first screen cloth with the second screen cloth order set up in the passageway just first screen cloth is located the upper reaches of second screen cloth, first screen cloth with the second screen cloth has a plurality of sieve meshes respectively, the sieve mesh aperture of first screen cloth is greater than the sieve mesh aperture of second screen cloth. The utility model discloses a capture device of biomolecule, cell or bacterium can avoid the influence of impurity to the specificity capture of biomolecule, cell or bacterium.

Description

Device for capturing biomolecules, cells or bacteria

Technical Field

The utility model relates to a capture device of biomolecule, cell or bacterium.

Background

In the detection of blood and biological samples, detection is often performed on rare cells and rare molecules in the detection sample, and the successful separation of these rare targets or molecules is the key to the accuracy of the detection. For example, in human production and life, detection of human body fluids, food and test objects in natural environments is required, and detection of cells and microorganisms is often required in medical practice, food safety and environmental monitoring. In addition, some proteins, nucleic acids, etc. are also often used as targets for detection, such as early diagnosis marker screening and gene diagnosis, etc. which are common in clinic. As with CTC cells, enrichment screening of CTC cells in blood can be performed by a capture device. The detection sample usually contains impurities, and the impurities can cause adverse effects on the capture of the CTC cells, such as interference detection, influence on detection precision and the like.

SUMMERY OF THE UTILITY MODEL

In view of the above technical problems, it is an object of the present invention to provide an improved capture device for biomolecules, cells or bacteria.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a biomolecule, cell or bacterium capturing device, comprising a clamp, wherein a channel for a sample to flow through is formed in the clamp, the capturing device further comprises a first capturing unit and a second capturing unit which are arranged in the clamp, the first capturing unit comprises a first screen for filtering impurities in the sample, the second capturing unit comprises a second screen for specifically binding with the biomolecule, cell or bacterium, the first screen and the second screen are sequentially arranged in the channel, the first screen is positioned at the upstream of the second screen, the first screen and the second screen respectively have a plurality of meshes, and the mesh aperture of the first screen is larger than that of the second screen.

Preferably, the first capturing unit or the second capturing unit further includes a first fixing layer and a second fixing layer, the first fixing layer and the second fixing layer are connected to fix the first screen or the second screen between the first fixing layer and the second fixing layer, and the middle portions of the first fixing layer and the second fixing layer respectively have a through hole for passing a liquid therethrough.

Preferably, a flexible sealing layer is arranged between the first screen or the second screen and the first fixing layer, a through hole for liquid to pass through is formed in the middle of the flexible sealing layer, and the flexible sealing layer has elasticity.

Preferably, first capture unit is from last to being in proper order down first fixed bed flexible seal membrane first screen cloth and the second fixed bed, the second capture unit is from last to being in proper order down first fixed bed flexible seal membrane the second screen cloth and the second fixed bed, first capture unit and second capture unit equipment are convenient and have good leakproofness.

More preferably, the first fixing layer is provided with a connecting hole, the second fixing layer is provided with a connecting column, and the connecting column is inserted into the connecting hole.

Preferably, the second fixed layer has the upper surface and certainly the recess of upper surface downwardly extending, the spliced pole certainly upper surface upwardly extending, first screen cloth or the second screen cloth is arranged in the recess, first fixed layer has the lower surface and certainly the arch of lower surface downwardly extending, the arch is inserted in the recess, flexible sealing layer cover in on the arch.

Preferably, the connecting holes and the connecting columns are respectively multiple, the connecting holes are arranged around the grooves at intervals, and the connecting columns are arranged around the protrusions at intervals.

More preferably, the flexible sealing layer and the first fixing layer are integrally provided.

Preferably, a plurality of positioning holes are formed in the first fixing layer, and the flexible sealing layer is provided with a plurality of positioning protrusions inserted into the positioning holes.

Preferably, the clamp comprises a first clamp and a second clamp which are detachably connected, the first clamp is provided with a mounting groove, the first capture unit and the second capture unit are embedded in the first clamp, and the second clamp limits the first capture unit and the second capture unit in the mounting groove after the second clamp and the first clamp are connected.

More preferably, a capture layer capable of specifically adsorbing the biomolecules, cells or bacteria is provided on the second screen.

The utility model adopts the above scheme, compare prior art and have following advantage:

the utility model discloses a trapping apparatus for biomolecule, cell or bacterium, the mesh aperture of first screen cloth is greater than the mesh aperture and the first screen cloth of second screen cloth and sets up in the upper reaches of second screen cloth, is the impurity filtering through first screen cloth in with the sample earlier for during impurity can't enter into the second screen cloth, recycle the second screen cloth and make biomolecule, cell or bacterium rather than combining, avoided the impurity in the sample to the adverse effect that biomolecule, cell or bacterium specificity were caught.

Drawings

In order to more clearly illustrate the technical solution of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

Fig. 1 is a schematic perspective view of a clamp according to an embodiment of the present invention;

FIG. 2 is a cross-sectional view of a first angle of the clamp;

FIG. 3 is a cross-sectional view of a second angle of the clamp;

FIG. 4 is a schematic structural diagram of a first pinned layer;

FIG. 5 is a schematic structural diagram of a second pinned layer;

FIG. 6 is a schematic diagram of the first anchor layer connected to the second anchor layer;

fig. 7 is an enlarged schematic view of the second screen.

Wherein the content of the first and second substances,

1. a clamp; 11. a channel; 12. a first clamp; 121. mounting grooves; 13. a second clamp; 2. a first capturing unit; 21. a first screen; 211. screening holes; 3. a second capturing unit; 31. a second screen; 311. a trapping layer; 4. a first fixed layer; 41. connecting holes; 42. a lower surface; 421. a protrusion; 43. positioning holes; 5. a second fixed layer; 51. connecting columns; 52. an upper surface; 521. a groove; 6. a flexible sealing layer; 61. positioning the projection; 7. a seal ring; 8. and a through hole.

Detailed Description

The following detailed description of the preferred embodiments of the invention, taken in conjunction with the accompanying drawings, enables the advantages and features of the invention to be more readily understood by those skilled in the art. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. Furthermore, the technical features mentioned in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.

Referring to fig. 1 to 3, the present embodiment provides a biomolecule, cell or bacterium capturing device, which includes a holder 1, wherein a channel 11 for a sample to flow through is formed in the holder 1. The capturing device further comprises a first capturing unit 2 and a second capturing unit 3 arranged in the jig 1. Further, the jig 1 includes a first jig 12 and a second jig 13 detachably connected, the first jig 12 having a mounting groove 121. The first catching unit 2 and the second catching unit 3 are embedded in the first jig 12, and after the second jig 13 is coupled to the first jig 12, the second jig 12 restrains the first catching unit 2 and the second catching unit 3 in the mounting groove 121. Specifically, the lower portion of the first clamp 12 is inserted into the upper portion of the second clamp 13, the first clamp 12 is provided with an external thread, the second clamp 13 is provided with an internal thread, and the first clamp 12 and the second clamp 13 are connected through a thread.

Referring to fig. 3, the first capture unit 2 includes a first screen 21 for filtering impurities in a sample, and the second capture unit 3 has a second screen 31 for specific binding with biomolecules, cells, or bacteria. As shown in fig. 7, the second mesh 31 is provided with a capturing layer 311 capable of specifically adsorbing biomolecules, cells, or bacteria. The capture layer 311 includes a capture object capable of specifically binding to a target cell, bacteria, or biomolecule. Specifically, the capture objects are bound to the second screen 31 by physical adsorption and/or chemical bonding. Preferably, the capture layer 311 is attached to the second screen 31 by using a traut's reagent or thiolate molecules with biotin-avidin.

The first screen 21 and the second screen 31 in the first jig 12 described above are sequentially disposed in the passage 11 with the first screen 21 located upstream of the second screen 31. That is, the sample passes through the first screen 31 to filter out impurities, and then passes through the second screen 32 to capture biomolecules, cells or bacteria and specifically bind thereto. It should be noted that the first screen 21 and the second screen 31 each have a plurality of openings 211, and the openings 211 of the first screen 21 are larger than the openings 211 of the second screen 31.

Further, the first capturing unit 2 further includes a first fixing layer 4 and a second fixing layer 5, the first fixing layer 4 and the second fixing layer 5 are connected to fix the first screen 21 between the first fixing layer 4 and the second fixing layer 5, and the middle portions of the first fixing layer 4 and the second fixing layer 5 are respectively provided with a through hole 8 for passing liquid. Similarly, the second capturing unit 3 includes a first fixing layer 4 and a second fixing layer 5, the first fixing layer 4 and the second fixing layer 5 are connected to fix the second screen 31 between the first fixing layer 4 and the second fixing layer 5, and the middle portions of the first fixing layer 4 and the second fixing layer 5 respectively have a through hole 8 for passing the liquid therethrough.

Further, a flexible sealing layer 6 is disposed between the first screen 21 and the first fixing layer 4, a flexible sealing layer 6 is also disposed between the second screen 31 and the first fixing layer 4, a through hole for passing liquid is formed in the middle of the flexible sealing layer 6, and the flexible sealing layer 6 has elasticity. The first fixing layer 4 is provided with a plurality of positioning holes 43, the flexible sealing layer 6 is provided with a plurality of positioning protrusions 61, the positioning protrusions 61 are inserted into the positioning holes 43, and the flexible sealing layer 6 and the first fixing layer 4 are integrally arranged, for example, formed by two-material co-extrusion. The first fixing layer 4 and the second fixing layer 5 are made of hard plastics, and the flexible sealing layer 6 is made of soft rubber. That is, the first fixing layer 4, the flexible sealing layer 6 and the second fixing layer 5 are annular as a whole, and the first screen 21 or the second screen 31 is circular or rectangular as a whole, has an area smaller than the outer diameters of the first fixing layer 4 and the second fixing layer 5, and covers the through hole portions of the three middle portions of the first fixing layer 4, the flexible sealing layer 6 and the second fixing layer 5, which are part of the channel 11 for the sample to flow through.

In addition to this, the catching device is provided with a plurality of annular sealing rings 7. Specifically, the upper surface of the first capture unit 2 and the lower surface of the second capture unit 3 are provided with annular grooves, and the seal rings 7 are disposed in the grooves.

Referring to fig. 4 to 6, the first fixing layer 4 is provided with a plurality of connection holes 41, the second fixing layer 5 is provided with a plurality of connection posts 51, and each connection post 51 is inserted into the corresponding connection hole 41. The second fixing layer 5 has an upper surface 52 and a groove 521 extending downward from the upper surface 52, the connecting column 51 extends upward from the upper surface 52, and the first screen 21 or the second screen 31 in the first fixture 12 is disposed in the groove 521. The first fixing layer 4 has a lower surface 42 and a protrusion 421 extending downward from the lower surface 42, the protrusion 421 is inserted into the groove 521, and the flexible sealing layer 6 covers the protrusion 421. A plurality of attachment holes 41 are spaced around the recess 521 and a plurality of attachment posts 51 are spaced around the projection 421.

The utility model discloses a capture device of biomolecule, cell or bacterium includes anchor clamps 1, has first capture element 2 and second capture element 3 in anchor clamps 1. The first capturing unit 2 is respectively provided with a first fixing layer 4, a flexible sealing layer 6, a first screen 21 and a second fixing layer 5 from top to bottom, and the second capturing unit 3 is respectively provided with a first fixing layer 4, a flexible sealing layer 6, a second screen 31 and a second fixing layer 5 from top to bottom. The connecting column 51 of the second fixing layer 5 is inserted into the connecting hole 41 of the first fixing layer 4, the protrusion 421 of the first fixing layer 4 is inserted into the groove 521 of the second fixing layer 5, the first screen 21 or the second screen 31 is arranged in the groove 521, the flexible sealing layer 6 is arranged between the first fixing layer 4 and the first screen 21 and between the first fixing layer 4 and the second screen 31, and the positioning protrusions 61 on the flexible sealing layer 6 are inserted into the positioning holes 43 of the first fixing layer 4, so that a module is integrally formed, and the assembly is convenient. And a flexible sealing layer 6 is provided between the first fixing layer 4 and the first screen 21 or the second screen 31, so that the sealing property of the jig 1 is good.

After the sample flows into the channel 11, the sample passes through the first screen 21, impurities in the sample are screened out through the screen holes 211 of the first screen, the sample after being screened out of the impurities flows into the second screen 31, and biomolecules, cells or bacteria are specifically bound to the second screen 31. That is, the capturing device of the present embodiment prevents impurities from affecting the result of specific capturing of biomolecules, cells, or bacteria by removing impurities.

As used in this specification and the appended claims, the terms "comprises" and "comprising" are intended to only encompass the explicitly identified steps and elements, which do not constitute an exclusive list, and that a method or apparatus may include other steps or elements. As used herein, the term "and/or" includes any combination of one or more of the associated listed items.

It should be noted that, unless otherwise specified, when a feature is referred to as being "fixed" or "connected" to another feature, it may be directly fixed or connected to the other feature or indirectly fixed or connected to the other feature. Furthermore, the description of the upper, lower, left, right, etc. used in the present invention is only relative to the mutual positional relationship of the components of the present invention in the drawings.

The above embodiments are only for illustrating the technical concept and features of the present invention, and are preferred embodiments, which are intended to enable persons skilled in the art to understand the contents of the present invention and to implement the present invention, and thus, the protection scope of the present invention cannot be limited thereby. All equivalent changes or modifications made according to the principles of the present invention are intended to be covered by the scope of the present invention.

Claims

1. A biomolecule, cell or bacterium capturing device, comprising a clamp, wherein a channel for a sample to flow through is formed in the clamp, and the capturing device is characterized by further comprising a first capturing unit and a second capturing unit which are arranged in the clamp, wherein the first capturing unit comprises a first screen for filtering impurities in the sample, the second capturing unit comprises a second screen for specifically binding with the biomolecule, cell or bacterium, the first screen and the second screen are sequentially arranged in the channel, the first screen is positioned at the upstream of the second screen, the first screen and the second screen respectively have a plurality of sieve holes, and the sieve hole diameter of the first screen is larger than that of the second screen.

2. The capturing apparatus according to claim 1, wherein the first capturing unit or the second capturing unit further includes a first fixing layer and a second fixing layer, the first fixing layer and the second fixing layer are connected to fix the first screen or the second screen between the first fixing layer and the second fixing layer, and the first fixing layer and the second fixing layer respectively have a through hole in a middle thereof through which a liquid passes.

3. The capturing device according to claim 2, wherein a flexible sealing layer is provided between the first screen or the second screen and the first fixing layer, a central portion of the flexible sealing layer has a through hole for passing a liquid therethrough, and the flexible sealing layer has elasticity.

4. The capturing device according to claim 3, wherein the first fixing layer is provided with a connecting hole, and the second fixing layer is provided with a connecting column, and the connecting column is inserted into the connecting hole.

5. The capturing device of claim 4, wherein the second securing layer has an upper surface and a groove extending downwardly from the upper surface, the connecting post extends upwardly from the upper surface, the first screen or the second screen is disposed in the groove, the first securing layer has a lower surface and a protrusion extending downwardly from the lower surface, the protrusion is inserted into the groove, and the flexible sealing layer covers the protrusion.

6. The capturing device of claim 5, wherein the connecting holes and the connecting posts are respectively a plurality of, the plurality of connecting holes being spaced around the groove, and the plurality of connecting posts being spaced around the protrusion.

7. A capture device according to claim 3, wherein the flexible sealing layer and the first fixing layer are provided integrally.

8. The capturing device of claim 7, wherein the first securing layer has a plurality of locating holes formed therein, and the flexible sealing layer has a plurality of locating protrusions inserted into the locating holes.

9. The capture device of claim 1, wherein the clamp includes a first clamp and a second clamp that are removably coupled, the first clamp having a mounting slot, the first capture unit and the second capture unit being nested within the first clamp, the second clamp restraining the first capture unit and the second capture unit within the mounting slot after the second clamp and the first clamp are coupled.

10. The capture device of claim 1, wherein the second screen is provided with a capture layer capable of specifically adsorbing the biomolecules, cells or bacteria.