CN217377871U

CN217377871U - Sealed cryopreservation pipe

Info

Publication number: CN217377871U
Application number: CN202220162511.3U
Authority: CN
Inventors: 刘家陆; 吕荣威; 霍小利; 王晓雨; 胡映莉; 刘晓明
Original assignee: Youji Biotechnology Hangzhou Co ltd
Current assignee: Youji Biotechnology Hangzhou Co ltd
Priority date: 2022-01-20
Filing date: 2022-01-20
Publication date: 2022-09-06
Anticipated expiration: 2032-01-20

Abstract

The utility model belongs to the technical field of it is biological, especially, relate to a sealed cryopreserving pipe. It includes: the tube body is provided with a containing cavity filled with reagents, and the tube cover is used for sealing an opening of the containing cavity; the pipe cover comprises an outer cover and an inner cover, and the outer cover and the outer wall of the pipe body are installed in a matched mode through first internal threads; the lower end face of the outer cover is also provided with an inner cylinder of which the inner surface is provided with a second internal thread, and the inner cover is in threaded fit with the inner cylinder; the rotating directions of the first internal thread and the second internal thread are opposite; the inner cup lower extreme is equipped with vertical bar bayonet socket, holds the intracavity wall and is equipped with the card protruding with bar bayonet socket complex. The utility model discloses technical scheme forms double seal through enclosing cover and inner cup complex compound tube cap at first, has improved holistic leakproofness, and on the other hand passes through the structure cooperation for the in-process that the tube cap screwed after the lid closes holds the intracavity in the pipe shaft and forms the negative pressure, and the formation of negative pressure can effectively restrain the biological reagent and keep or the leakage that the PCR amplification in-process produced for a long time.

Description

Sealed cryopreservation pipe

Technical Field

The utility model belongs to the technical field of it is biological, especially, relate to a sealed cryopreserving pipe.

Background

A cryopreservation tube is a container for storing a biological sample and placing the biological sample in a suitable freezing medium such as liquid nitrogen for cryopreservation of the biological sample, and is used for transportation and storage, and also can be directly used for PCR amplification treatment, and part of the cryopreservation tube is actually required to carry out PCR amplification treatment.

Most of the existing PCR freezing tubes only have simple sealing effect and are used for preventing pollution to the outside, the actual air tightness cannot be effectively guaranteed, and no problem exists when the existing PCR freezing tubes are used for temporary storage under conventional conditions. However, when the method is used for long-term storage or amplification treatment in a PCR amplification apparatus, aerosol leakage is very likely to occur due to increase in volatilization amount caused by aging or increase in volatilization amount caused by temperature accompanying increase in air pressure in the tube, and finally aerosol cross contamination among a plurality of biological reagents is caused.

Disclosure of Invention

For solving current PCR cryopreserved pipe gas tightness not good, produce the leakage and cross contamination scheduling problem of biological reagent easily when preserving biological reagent for a long time or being used for PCR amplification equipment, the utility model provides a sealed cryopreserved pipe.

The utility model aims to provide an:

firstly, improving the sealing performance of the freezing storage pipe;

and secondly, the using portability of the freezing storage tube is ensured.

In order to achieve the purpose, the utility model discloses technical scheme below the sampling.

A sealed cryopreservation tube comprising:

the tube body is provided with a containing cavity filled with reagents, and the tube cover is used for sealing an opening of the containing cavity;

the pipe cover comprises an outer cover and an inner cover, the edge of the outer cover extends downwards to form a pipe shape, a first internal thread is arranged on the inner wall of the pipe shape part, and the outer cover and the outer wall of the pipe body are screwed and installed in a threaded fit mode;

the lower end face of the outer cover is also provided with an inner cylinder, the inner surface of the inner cylinder is provided with a second internal thread, and the upper end part of the inner cover is in threaded fit with the inner cylinder; the rotating directions of the first internal thread and the second internal thread are opposite;

the inner cup lower extreme is equipped with vertical bar bayonet socket, holds the intracavity wall and is equipped with the card protruding with bar bayonet socket complex, and bar bayonet socket is greater than the protruding vertical size of card in the ascending length of vertical direction.

Preferably, the length of the first internal thread is smaller than that of the second internal thread, and the pitch of the first internal thread is larger than that of the second internal thread; and the side wall of the inner cover is abutted against the cavity wall to be in sealing contact with the cavity wall after the pipe cover is covered.

As a preference, the first and second liquid crystal compositions are,

the tubular portion conforms to the outer wall of the body.

As a preference, the first and second liquid crystal compositions are,

the outer cover is provided with capillary air holes.

As a matter of preference,

rubber fillers are arranged in the strip-shaped bayonets.

As a preference, the first and second liquid crystal compositions are,

the outer side wall of the pipe body is provided with anti-skid lines.

The utility model has the advantages that:

1) the technical scheme of the utility model is that the composite pipe cover with the matched outer cover and inner cover forms double sealing, so as to improve the whole sealing performance, and on the other hand, through structure matching, the pipe cover forms negative pressure in the cavity in the pipe body in the screwing process after covering, and the formation of the negative pressure can effectively inhibit the leakage of the biological reagent in the long-term maintenance or PCR amplification process;

2) the whole use is no different from the existing conventional freezing tube, can be used for using partial volatile biological reagents, and has good use portability.

Drawings

Fig. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic cross-sectional view of portion A-A of FIG. 1;

FIG. 3 is a schematic view of the tube body separated from the tube cover;

FIG. 4 is an enlarged view of portion B of FIG. 3;

FIG. 5 is a schematic view of the volume and pressure change of the tubular body cavity during the closing and tightening of the cap;

FIG. 6 is an enlarged view of portion C of FIG. 5;

in the figure: 100 pipe bodies, 101 clamping protrusions, 102 anti-skidding threads, 200 pipe covers, 201 outer covers, 2011 first internal threads, 2012 capillary air holes, 2013 inner cylinders, 20131 second internal threads, 202 inner covers, 2021 strip-shaped bayonets and 2022 rubber fillers.

Detailed Description

The invention is described in further detail below with reference to specific embodiments and drawings. Those of ordinary skill in the art will be able to implement the present invention based on these descriptions. Furthermore, the embodiments of the invention described in the following description are generally only some embodiments, but not all embodiments of the invention. Therefore, all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention shall fall within the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "thickness", "upper", "lower", "horizontal", "top", "bottom", "inner", "outer", "circumferential", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are used merely for convenience of description and simplification of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., and "a plurality" means one or more unless specifically limited otherwise.

In the present invention, unless otherwise explicitly specified or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly, e.g., as being fixedly connected, detachably connected, or integrated; may be mechanically coupled, may be electrically coupled or may be in communication with each other; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

Examples

A sealed cryopreservation tube as shown in fig. 1 and 3, comprising:

the tube body 100 is provided with a cavity with an opening at the upper end, and the cavity is used for filling and bearing biological reagents needing to be stored or reacted;

the outer side wall of the pipe body 100 is optionally provided with anti-skid threads 102;

the tube cover 200 is detachably fitted on the tube body 100 to close the upper end opening of the cavity;

the pipe cover 200 is a pressure-adjustable pipe cover 200, and is specifically divided into an outer cover 201 and an inner cover 202 as shown in fig. 1, the edge of the outer cover 201 extends downwards to form a pipe shape, a first internal thread 2011 is arranged on the inner wall of the pipe-shaped part, the outer cover 201 is screwed and installed with the outer wall of the pipe body 100 through thread fit, and the pipe-shaped part is attached to the outer wall of the pipe body 100 after installation;

an inner cylinder 2013 circumferentially covered by a tubular part is further arranged on the lower end face of the outer cover 201, the upper end part of the inner cover 202 is in threaded fit with the inner cylinder 2013, specifically, a second internal thread 20131 is arranged on the inner surface of the inner cylinder 2013, as shown in fig. 4, the length of the first internal thread 2011 in the vertical direction is smaller than that of the second internal thread 20131, but the pitch r1 of the first internal thread 2011 is larger than the pitch r2 of the second internal thread 20131, the screwing directions of the first internal thread 2011 and the second internal thread 20131 are opposite, and after the tube cover 200 is covered, the side wall of the inner cover 202 abuts against the wall of the cavity and is in sealing contact with the wall of the cavity;

the lower end of the inner cover 202 is provided with a vertical strip-shaped bayonet 2021, the inner wall of the cavity is provided with a clamping protrusion 101 matched with the strip-shaped bayonet 2021, the strip-shaped bayonet 2021 and the clamping protrusion 101 are in clamping fit as shown in fig. 1 and fig. 2, the clamping protrusion 101 limits the rotation of the inner cover 202 after the clamping fit, and the length of the strip-shaped bayonet 2021 in the vertical direction is larger than that of the clamping protrusion 101;

when the tube cap 200 is not covered, the top end of the inner cap 202 is separated from the bottom end of the outer cap 201, and the inner cap 202 is screwed down to the lowest end or a position close to the lowest end of the second thread segments, i.e. the state shown in fig. 3;

under the cooperation of the above structure, as shown in fig. 5, firstly, the strip-shaped bayonet 2021 of the inner cap 202 of the tube cap 200 is aligned with the clamping protrusion 101 on the inner wall of the cavity of the tube body 100, so as to achieve a preliminary clamping fit, and the tube cap 200 is integrally and preliminarily covered on the tube body 100, then the outer cap 201 is screwed, the outer cap 201 is fitted with the tube body 100 through the first internal thread 2011 so as to achieve a threaded installation and screwing, so as to form an outer seal, in the process, the inner cap 202 cannot rotate along with the outer cap 201 due to the fact that the second internal thread 20131 of the inner tube 2013 is opposite to the first internal thread 2011 of the tubular portion of the outer cap 201, in the process of screwing the outer cap 201 downwards, since the inner cap 202 is limited by the clamping protrusion 101, so that the inner cap 202 and the outer cap 201 are relatively rotated due to the action of the second internal thread 20131, so that the inner cap 202 moves upwards, and since the inner cap 202 already forms an internal seal, in the process of moving upwards, the volume in the cavity is increased but external air cannot be supplemented, so that a negative pressure environment is formed, when alpha is initially covered as shown in 5-1 in fig. 5, the cavity is in a normal pressure environment, and beta is in a negative pressure environment as shown in 5-2 in fig. 5 after the pipe cover 200 is screwed, the formed negative pressure environment can effectively prevent the reagent in the cryopreservation pipe from volatilizing to generate aerosol and leaking, and meanwhile, the pipe cover 200 and the pipe body 100 are connected more tightly under the action of pressure difference, so that the sealing performance of the whole cryopreservation pipe is improved.

Further, in the above-mentioned case,

the outer cap 201 is provided with the capillary air holes 2012 which are communicated with the inner cap 202 up and down, and the arrangement of the capillary air holes 2012 can further improve the convenience of the screwing process of the tube cap 200, because it is found in practical use, and the screw-thread fit between the inner cap 202 and the outer cap 201 also has good air-tightness during the tightening process, so that the inner cap 202 is gradually fitted over the lower end surface of the outer cap 201, the gas between the gaps is difficult to discharge or even can not be discharged, so that larger gas pressure is generated between the gaps, even the inner cover 202 and the outer cover 201 can not be effectively attached under partial conditions, although not significantly affecting the seal, the significant increase in air pressure of the process results in the tightness of fit between the inner cap 202 and the inner barrel 2013 of the outer cap 201 being susceptible to, after the capillary air holes 2012 are arranged, the gas can be effectively exhausted, and meanwhile, the capillary air holes 2012 can form a certain isolation effect;

in addition, as shown in fig. 6, a rubber filler 2022 is further disposed at the uppermost end of the strip bayonet 2021, and the rubber filler 2022 can further ensure the sealing property when the tube cap 200 and the tube body 100 are preliminarily covered, thereby improving the use effect.

The sealed cryopreserving pipe of above-mentioned structure, have very big use value, present PCR cryopreserved pipe only guarantees gas tightness and plot nature simply usually, but when using in PCR amplification equipment, along with the rising of temperature and the volatilization of solvent, can produce great aerosol pressure in the actual PCR cryopreserved pipe, leads to partial volatile biological reagent to form the aerosol and leaks, causes aerosol cross contamination in PCR amplification equipment, nevertheless passes through the utility model discloses a sealed cryopreserved pipe has not only ensured that it has good conventional sealing performance, when being used for PCR amplification equipment, can avoid biological reagent to leak very effectively and lead to cross contamination's the condition to take place.

Claims

1. A sealed cryopreservation tube, comprising:

the lower end face of the outer cover is also provided with an inner cylinder, the inner surface of the inner cylinder is provided with a second internal thread, and the upper end part of the inner cover is in threaded fit with the inner cylinder;

the rotating directions of the first internal thread and the second internal thread are opposite;

2. The sealed cryopreservation tube of claim 1, wherein,

the length of the first internal thread is smaller than that of the second internal thread, and the pitch of the first internal thread is larger than that of the second internal thread; and the side wall of the inner cover is abutted against the cavity wall to be in sealing contact with the cavity wall after the pipe cover is covered.

3. The sealed vial of claim 1,

the tubular portion conforms to the outer wall of the body.

4. The sealed vial of claim 1,

the outer cover is provided with capillary air holes.

5. The sealed vial of claim 1,

rubber fillers are arranged in the strip-shaped bayonets.

6. A sealed cryopreservation tube as claimed in claim 1 or 2 or 3 or 4 or 5,

the outer side wall of the pipe body is provided with anti-skid lines.