CN217321351U

CN217321351U - Single cell exosome static detection kit

Info

Publication number: CN217321351U
Application number: CN202220202513.0U
Authority: CN
Inventors: 江海松; 胡建容
Original assignee: Hangzhou Huiyu Medical Technology Co ltd
Current assignee: Hangzhou Huiyu Medical Technology Co ltd
Priority date: 2022-01-25
Filing date: 2022-01-25
Publication date: 2022-08-30
Anticipated expiration: 2032-01-25

Abstract

The utility model discloses a unicellular exosome static state detect reagent box, including the box body, still include protection machanism, protection machanism is installed to the lateral wall of box body, protection machanism comprises apron, buckle, fixture block, foam-rubber cushion A, standing groove and the combination of foam-rubber cushion B, the lateral wall of box body passes through hinged joint with the lateral wall of apron, foam-rubber cushion B is installed to the lateral wall of apron, foam-rubber cushion A is installed to the inside wall of box body, the standing groove has been seted up to foam-rubber cushion A's lateral wall, the buckle is installed to the lateral wall of apron, the fixture block is installed to the lateral wall of box body. The protection mechanism can increase the protection performance on the reagent tube and avoid the damage of the reagent tube after collision; and the tightness of the reagent tube can be increased through the sampling mechanism, so that the leakage of the reagent tube is avoided.

Description

Single cell exosome static detection kit

Technical Field

The utility model relates to a detect reagent box technical field, in particular to unicellular exosome static state detect reagent box.

Background

The earliest prototype of flow cytometry was born in 1934, and Moldavan proposed the idea of flowing suspended individual red blood cells through a glass capillary, counting them under a bright field microscope, and measuring them with an electro-optical recording device. In 1953 Croslland-Taylor designed flow chambers according to the Newtonian fluid flow law in circular tubes. And then, the physical connection between a computer and the flow cytometer, the recording and analysis of multi-parameter data, the development of immunofluorescence staining and detection technology of cells and the popularization of the clinical application of the flow cytometer are carried out. In recent 20 years, with the increasingly perfection of flow cytometry and detection technology thereof, people are increasingly dedicated to the work in the aspects of sample preparation, cell marking, software development and the like so as to expand the application field and use effect of FCM.

Patent No. 202010553024.5 discloses a single-cell exosome static detection kit and a detection analysis method, wherein the kit comprises a cell phenotype analysis agent, an exosome detection agent and a microfluidic chip set; the cell phenotype analysis reagent comprises a specific cell protection fluorescent dye and a cell buffer solution; the agent for detecting the exosome comprises an exosome detection antibody, a fluorescent conjugate, an antibody buffer solution and a confining liquid; the micro-fluidic chip group comprises a micro-cavity array chip and an exosome capturing chip. The kit and the method disclosed by the invention can be used for detecting and analyzing trace samples and carrying out static phenotype analysis and exosome detection on suspension cells and adherent cells, and have the advantages of high detection flux and accurate and reliable detection result.

The single-cell exosome static detection kit has the following defects: 1. the device has poor protection performance, so that the reagent tube is easy to damage after collision; 2. the poor sealing of the reagent tube results in a reagent tube that is prone to leakage. Therefore, we propose a single cell exosome static detection kit.

SUMMERY OF THE UTILITY MODEL

The utility model mainly aims at providing a single cell exosome static detection kit, which can increase the protection performance to a reagent tube through a protection mechanism and avoid the reagent tube from being damaged after collision; the leak tightness of the reagent tube can be increased through the sampling mechanism, the leakage of the reagent tube is avoided, and the problem in the background art can be effectively solved.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides a unicellular exosome static detection kit, includes the box body, still includes protection machanism, protection machanism is installed to the lateral wall of box body, protection machanism comprises apron, buckle, fixture block, foam-rubber cushion A, standing groove and foam-rubber cushion B combination, the lateral wall of box body passes through hinged joint with the lateral wall of apron, foam-rubber cushion B is installed to the lateral wall of apron, foam-rubber cushion A is installed to the inside wall of box body, the standing groove has been seted up to foam-rubber cushion A's lateral wall, the buckle is installed to the lateral wall of apron, the fixture block is installed to the lateral wall of box body.

Further, still include sampling mechanism, sampling mechanism has been placed to the inside wall of box body, and sampling mechanism conveniently gathers the exosome.

Further, the sampling mechanism comprises reagent pipe, rubber head, thread groove and screw cap combination, the thread groove has been seted up to the lateral wall of reagent pipe, the lateral wall threaded connection of thread groove has the screw cap, the one end in-connection of reagent pipe has the rubber head, seals the reagent pipe through the rubber head, avoids the exosome seepage.

Further, the reagent tube is located in the placing groove and can be fixed to the reagent tube.

Furthermore, the buckle is mutually matched with the clamping block, so that the buckle and the clamping block are conveniently connected.

Compared with the prior art, the utility model discloses following beneficial effect has: 1. the outer side wall of the box body is provided with a protection mechanism, the protection mechanism is composed of a cover plate, a buckle, a clamping block, a sponge cushion A, a placing groove and a sponge cushion B, the outer side wall of the box body is connected with the outer side wall of the cover plate through a hinge, so that the cover plate can rotate on the outer side wall of the box body, the sponge cushion A is installed on the inner side wall of the box body, the placing groove is formed in the outer side wall of the sponge cushion A, a reagent tube is positioned in the placing groove, so that the reagent tube can be fixed, the sponge cushion B is installed on the outer side wall of the cover plate, so that the cover plate is rotated, the sponge cushion B is attached to the top of the reagent tube, the buckle is installed on the outer side wall of the cover plate, the clamping block is installed on the outer side wall of the box body, the buckle is matched with the clamping block, so that the reagent tube is conveniently sealed, and the reagent tube is prevented from being damaged;

2. sampling mechanism has been placed to the inside wall of box body, sampling mechanism is by the reagent pipe, the rubber head, thread groove and screw cap combination constitute, personnel gather and place the exosome in the intraductal back of reagent, because the rubber head matches with the one end of reagent pipe, thereby can seal the one end of reagent pipe in inserting the one end of reagent pipe with the rubber head, because the thread groove has been seted up to the lateral wall of reagent pipe, thereby with screw cap and thread groove fastening connection, can effectually seal the one end of reagent pipe, avoid exosome seepage.

Drawings

FIG. 1 is a schematic diagram of the overall structure of the single-cell exosome static detection kit of the present invention.

Fig. 2 is the structure schematic diagram of the protection mechanism of the single cell exosome static detection kit of the utility model.

FIG. 3 is the utility model discloses a sampling mechanism structure sketch map of unicellular exosome static detection kit

In the figure: 1. a case body; 2. a protection mechanism; 201. a cover plate; 202. buckling; 203. a clamping block; 204. a spongy cushion A; 205. a placement groove; 206. a spongy cushion B; 3. a sampling mechanism; 301. a reagent tube; 302. a rubber head; 303. a thread groove; 304. and (4) a threaded cap.

Detailed Description

In order to make the technical means, creation features, achievement purposes and functions of the present invention easy to understand, the present invention is further described below with reference to the following embodiments.

As shown in fig. 1-3, a unicellular exosome static detection kit, includes box body 1, still includes protection machanism 2, protection machanism 2 is installed to the lateral wall of box body 1, protection machanism 2 comprises apron 201, buckle 202, fixture block 203, foam-rubber cushion A204, standing groove 205 and foam-rubber cushion B206 combination, the lateral wall of box body 1 passes through hinged joint with the lateral wall of apron 201, foam-rubber cushion B206 is installed to the lateral wall of apron 201, foam-rubber cushion A204 is installed to the inside wall of box body 1, standing groove 205 has been seted up to the lateral wall of foam-rubber cushion A204, buckle 202 is installed to the lateral wall of apron 201, fixture block 203 is installed to the lateral wall of box body 1.

Wherein, still include sampling mechanism 3, sampling mechanism 3 has been placed to the inside wall of box body 1, and sampling mechanism 3 conveniently gathers the exosome.

Wherein, sampling mechanism 3 comprises reagent pipe 301, rubber head 302, thread groove 303 and the combination of screw cap 304, the thread groove 303 has been seted up to the lateral wall of reagent pipe 301, the lateral wall threaded connection of thread groove 303 has screw cap 304, the one end in-connection of reagent pipe 301 has rubber head 302, seals reagent pipe 301 through rubber head 302, avoids the leakage of exosome.

The reagent tube 301 is positioned in the placement tank 205, and the reagent tube 301 can be fixed.

The buckle 202 and the fixture block 203 are matched with each other, so that the buckle 202 and the fixture block 203 are conveniently connected.

It should be noted that, the utility model relates to a unicellular exosome static detection kit, in operation, the outer sidewall of the box body 1 is provided with the protection mechanism 2, the protection mechanism 2 is composed of a cover plate 201, a buckle 202, a clamping block 203, a sponge pad A204, a placement groove 205 and a sponge pad B206, the outer sidewall of the box body 1 is connected with the outer sidewall of the cover plate 201 through a hinge, so that the cover plate 201 can rotate on the outer sidewall of the box body 1, because the inner sidewall of the box body 1 is provided with the sponge pad A204, the outer sidewall of the sponge pad A204 is provided with the placement groove 205, the reagent tube 301 is positioned in the placement groove 205, so that the reagent tube 301 can be fixed, the outer sidewall of the cover plate 201 is provided with the sponge pad B206, so that the sponge pad B206 is attached to the top of the reagent tube 301 by rotating the cover plate 201, because the outer sidewall of the cover plate 201 is provided with the buckle 202, the clamping block 203 is installed on the outer sidewall of the box body 1, the buckle 202 is matched with the clamping block 203, thereby through buckle 202 and fixture block 203 interconnect, conveniently seal reagent pipe 301, avoid reagent pipe 301 to damage, sampling mechanism 3 has been placed to the inside wall of box body 1, sampling mechanism 3 is by reagent pipe 301, rubber head 302, thread groove 303 and the combination of screw cap 304 constitute, personnel gather and place the exosome in reagent pipe 301 back, because rubber head 302 matches with the one end of reagent pipe 301, thereby insert rubber head 302 and can seal reagent pipe 301's one end in reagent pipe 301's one end, thread groove 303 has been seted up to the lateral wall of reagent pipe 301, thereby be connected screw cap 304 and thread groove 303 fastening, can effectually seal reagent pipe 301's one end, avoid the exosome seepage.

The basic principles and the main features of the invention and the advantages of the invention have been shown and described above. It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The utility model provides a static detect reagent box of unicellular exosome, includes box body (1), its characterized in that still includes safeguard mechanism (2), safeguard mechanism (2) are installed to the lateral wall of box body (1), safeguard mechanism (2) are by apron (201), buckle (202), fixture block (203), foam-rubber cushion A (204), standing groove (205) and foam-rubber cushion B (206) combination constitution, the lateral wall of box body (1) passes through hinged joint with the lateral wall of apron (201), foam-rubber cushion B (206) are installed to the lateral wall of apron (201), foam-rubber cushion A (204) are installed to the inside wall of box body (1), standing groove (205) have been seted up to the lateral wall of foam-rubber cushion A (204), buckle (202) are installed to the lateral wall of apron (201), fixture block (203) are installed to the lateral wall of box body (1).

2. The single-cell exosome static detection kit according to claim 1, characterized in that: still include sampling mechanism (3), sampling mechanism (3) have been placed to the inside wall of box body (1).

3. The single-cell exosome static detection kit according to claim 2, characterized in that: sampling mechanism (3) comprise reagent pipe (301), rubber head (302), thread groove (303) and screw cap (304) combination, thread groove (303) have been seted up to the lateral wall of reagent pipe (301), the lateral wall threaded connection of thread groove (303) has screw cap (304), one end in-connection of reagent pipe (301) has rubber head (302).

4. The static single-cell exosome detection kit according to claim 3, wherein: the reagent tube (301) is located in the placement groove (205).

5. The single-cell exosome static detection kit according to claim 1, characterized in that: the buckle (202) and the fixture block (203) are matched with each other.