CN217289528U - Automatic membrane washing device for western blotting - Google Patents

Abstract

The utility model relates to an automatic membrane device of washing for western blotting, including stock solution unit, reposition of redundant personnel unit, wash membrane unit, tapping unit, collection liquid unit and the control unit. The liquid storage unit is used for storing the membrane washing liquid; the shunting unit is arranged at the downstream of the liquid storage unit and is used for uniformly shunting the membrane washing liquid; the membrane washing unit is arranged at the downstream of the shunting unit and used for storing membrane washing liquid for membrane washing; the liquid discharging unit is arranged on the front side of the interior of the membrane washing unit, is in sliding connection with the interior of the membrane washing unit and is used for opening or closing the membrane washing unit; the liquid collecting unit is horizontally arranged on the horizontal shaking bed, and the outer wall of the top of the liquid collecting unit is connected with the outer wall of the bottom of the membrane washing unit and used for collecting waste liquid after membrane washing; the control unit is connected with the liquid storage unit, the shunting unit and the liquid discharging unit and is used for controlling the device to wash and discharge liquid; the device is simple and quick to operate, saves time and avoids material waste.

Description

Automatic membrane washing device for western blotting

Technical Field

The utility model relates to a biochemical detection technology field especially relates to an automatic membrane device of washing for western blotting.

Background

Western blotting is a hybridization technique that combines high resolution gel electrophoresis with immunochemical analysis techniques. The immunoblotting method has the advantages of large analysis capacity, high sensitivity, strong specificity and the like, and is the most common method for detecting protein characteristics, expression and distribution, such as qualitative and quantitative detection of tissue antigens, quality determination of polypeptide molecules, detection of antibodies or antigens of viruses and the like.

Membrane washing is an essential process in a western blotting experiment. The common membrane washing device is similar to an experimental western blot membrane washing device disclosed in the patent application No. CN202021523974.5, the device mainly comprises a membrane washing liquid bottle and a membrane washing box, a liquid outlet is formed in the lower portion of the membrane washing liquid bottle, a flow inlet hole and a flow outlet are formed in the membrane washing box, the height of the liquid outlet is higher than that of the flow inlet hole, the height of the flow inlet hole is higher than that of the flow outlet hole, and the liquid outlet is communicated with the flow inlet hole. The membrane washing waste liquid does not need to be poured each time, the efficiency is improved, and the manpower resource and the time cost are reduced; the dynamic update of the membrane washing liquid can improve the membrane washing effect and reduce the pollution caused by misoperation in the process of manual operation.

However, the membrane washing device for the western blotting still has some defects in the use process and needs to be improved: the operation of filling the membrane washing solution into the membrane washing bottle is complex; a plurality of membrane washing boxes work simultaneously, and the dosage of membrane washing liquid in different membrane washing boxes can not be controlled. And the condition that a plurality of membrane washing boxes discharge waste liquid simultaneously cannot be met; the dynamic update of the membrane washing liquid in the membrane washing box causes the waste of the membrane washing liquid.

At present, effective solutions are not provided aiming at the problems that different membrane washing boxes are wasted, different quantities of membrane washing solutions cannot be injected simultaneously into the different membrane washing boxes, waste liquid can not be discharged simultaneously from a plurality of membrane washing boxes, the operation of filling the membrane washing solutions by a device is complex and the like in the prior art.

SUMMERY OF THE UTILITY MODEL

The utility model aims at not enough among the prior art, provide an automatic washing membrane device for albumen immunoblotting to solve the washing membrane liquid that exists among the correlation technique extravagant, can't satisfy different washing membrane boxes and pour into the washing membrane liquid of inequality simultaneously, can't satisfy a plurality of washing membrane boxes and discharge the operation complicacy scheduling problem of waste liquid, device packing washing membrane liquid simultaneously.

In order to achieve the purpose, the utility model adopts the technical proposal that:

the utility model provides an automatic membrane device of washing for western blotting, its characterized in that, includes stock solution unit, reposition of redundant personnel unit, washes membrane unit, tapping unit, album liquid unit and the control unit, wherein:

the liquid storage unit is used for storing membrane washing liquid;

the shunting unit is arranged at the downstream of the liquid storage unit and is used for uniformly shunting the membrane washing liquid;

the membrane washing unit is arranged at the downstream of the shunting unit and used for storing the membrane washing liquid for membrane washing;

the liquid discharging unit is arranged on the front side of the interior of the membrane washing unit, is in sliding connection with the interior of the membrane washing unit and is used for opening or closing the membrane washing unit;

the liquid collecting unit is horizontally arranged on the horizontal shaking bed, and the outer wall of the top of the liquid collecting unit is connected with the outer wall of the bottom of the membrane washing unit and used for collecting waste liquid after membrane washing;

the control unit is respectively connected with the liquid storage unit, the shunting unit and the liquid discharging unit and used for controlling the shunting unit to convey the film washing liquid of the liquid storage unit to the film washing unit and controlling the liquid discharging unit to flow the film washing liquid of the film washing unit out to the liquid collecting unit.

Further, the stock solution unit includes support element, stock solution component, first inlet component, first outlet component and feed liquor lid component, wherein:

the liquid storage element is horizontally arranged at the top of the supporting element, and the outer wall of the bottom of the liquid storage element is connected with the outer wall of the top of the supporting element;

the first liquid inlet element is arranged at the top of one side of the liquid storage element;

the first liquid outlet element is a liquid outlet and is arranged at the middle lower part of the front side of the liquid storage element;

the liquid inlet cover element is arranged above the liquid storage element, is detachably connected with the outer surface of the first liquid inlet element and is used for opening or closing the first liquid inlet element.

Further, the flow distribution unit comprises a first flow guide element, a flow distribution element and a plurality of second flow guide elements, wherein:

the first drainage element is arranged at the downstream of the liquid storage unit, and an inlet of the first drainage element is connected with the liquid storage unit;

the flow dividing element is arranged at the downstream of the first drainage element, and an inlet of the flow dividing element is connected with an outlet of the first drainage element;

the second drainage elements are arranged at the downstream of the flow dividing element, inlets of the second drainage elements are connected with outlets of the flow dividing element, and outlets of the second drainage elements are connected with the membrane washing unit.

Further, the flow dividing unit further comprises a first clamping element and a plurality of second clamping elements, wherein:

the first clamping element is arranged on the outer surface of the inlet of the first drainage element and used for clamping and fixing the connection between the first drainage element and the liquid storage unit;

and the plurality of second clamping elements are respectively arranged on the outer surfaces of the outlets of the plurality of second drainage elements and used for clamping and fixing the second drainage elements and the membrane washing unit.

Further, wash membrane unit including washing membrane frame component, a plurality of separation recess component, a plurality of first baffle component, a plurality of membrane washing groove, a plurality of second inlet component, apron component, recess component, direction component and opening element, wherein:

the membrane washing frame element is arranged at the downstream of the flow dividing unit;

the plurality of separation groove elements are symmetrically arranged on the inner wall of the rear side of the membrane washing frame element and the middle front side in the membrane washing frame element at intervals;

the first baffle elements are vertically arranged inside the membrane washing frame element at intervals, the outer walls of the bottoms of the first baffle elements are connected with the inner wall of the bottom of the membrane washing frame element, and the outer walls of the two sides of each first baffle element are detachably connected with the inner wall of the separation groove element;

the membrane washing frame element is embedded in the membrane washing frame element, the outer wall of the bottom of the membrane washing frame element is connected with the inner wall of the membrane washing frame element, the outer wall of the membrane washing frame element is connected with the inner wall of the membrane washing frame element, and the rear side of the membrane washing frame element is connected with the outer wall of the front side of the separation groove elements;

the plurality of membrane washing grooves are arranged inside the membrane washing frame element, and the plurality of separation groove elements, the plurality of first baffle elements and the partition plate element divide the inner space of the membrane washing frame element into the plurality of membrane washing grooves;

the second liquid inlet elements are arranged on the outer wall of the middle upper part of the rear side of the membrane washing frame element at intervals and are connected with the shunting unit;

the cover plate element is arranged at the top opening of the film washing frame element and is hinged to one side of the top of the film washing frame element;

the groove element is arranged at the bottom of the membrane washing frame element and is used for detachably arranging the clapboard element;

the guide element is arranged on the inner wall of the front side of the membrane washing frame element;

the opening element is arranged on one side of the membrane washing frame element.

Further, wash membrane unit still includes a plurality of first drainage holes, a plurality of second drainage hole and a plurality of third drainage hole, wherein:

the first drainage holes are arranged on one side of the front side of the membrane washing grooves and are round openings arranged in the middle of one side of the horizontal bottom of the partition plate element;

the second drainage holes are arranged on the front sides of the film washing grooves, the second drainage holes and the first drainage holes are arranged on the same side, and the second drainage holes are rectangular sieving openings arranged at the bottoms of the vertical side walls of the partition plate elements and used for sieving materials in the film washing grooves;

a plurality of the third drainage holes are circular openings arranged on the bottom groove element of the membrane washing frame element, the aperture of each third drainage hole is larger than that of each first drainage hole, and the third drainage holes are arranged under the first drainage holes.

Further, the tapping unit comprises a second baffle element and a third baffle element, wherein:

the second baffle element is arranged inside the film washing unit and is in sliding connection with the guide element and the partition element of the film washing unit;

the third baffle element is vertically connected to one side of the second baffle element, one side of the third baffle element is connected with the outer wall of one side of the membrane washing unit during waste liquid discharge, and the third baffle element can vertically cover the rectangular opening of the membrane washing unit.

Further, the tapping unit further comprises a plurality of fourth drainage holes and a plurality of fifth drainage holes, wherein:

the plurality of fourth drainage holes are rectangular openings which are arranged at the bottom of the vertical side wall of the second baffle element at intervals, and the fourth drainage holes are communicated with the film washing unit when waste liquid is discharged;

and the fifth drainage holes are rectangular openings arranged at the horizontal bottom of the second baffle element at intervals, are communicated with the fourth drainage holes, and are communicated with the membrane washing unit when waste liquid is drained.

Further, the liquid collecting unit comprises a liquid collecting element, a plurality of sixth drainage holes, a second liquid outlet element and a liquid outlet cover element, wherein:

the liquid collecting element is horizontally arranged on the horizontal shaking table, and the outer wall of the top of the liquid collecting element is connected with the outer wall of the bottom of the membrane washing unit;

the plurality of sixth drainage holes are round openings formed in the top of the liquid collecting element, and the sixth drainage holes are communicated with the membrane washing unit;

the second liquid outlet element is arranged at the middle lower part of one side of the liquid collecting element;

the liquid outlet cover element is detachably connected with the outer surface of the second liquid outlet element and is used for opening or closing the second liquid outlet element.

Further, the control unit comprises a plurality of flow monitoring elements, a plurality of switching elements, a baffle control element and a timing element, wherein:

the flow monitoring elements are respectively arranged at the inlet and/or the outlet of the shunting unit and are used for metering the flow of membrane washing liquid of the shunting unit;

the switch elements are respectively arranged at the inlet and/or the outlet of the shunting unit and are used for controlling the flow and stop of the membrane washing liquid of the shunting unit;

the baffle control element is arranged on the liquid discharging unit, is connected with the liquid discharging unit and is used for controlling the liquid discharging unit so that the membrane washing liquid of the membrane washing unit flows out to the liquid collecting unit;

the timing element is arranged in the liquid storage unit, is respectively connected with the plurality of switch elements and the baffle control element, and is used for controlling the switch elements and the baffle control element.

The utility model adopts the above technical scheme, compare with prior art, have following technological effect:

(1) the device adopts the liquid storage unit to store the membrane washing liquid, and the operation of filling the liquid is simple and rapid;

(2) the device utilizes the sliding connection between the liquid discharging unit and the interior of the membrane washing unit to open or close the membrane washing unit so that a plurality of membrane washing grooves in the device can simultaneously discharge liquid or wash membranes;

(3) the device divides the interior of the membrane washing unit into a plurality of grooves with opposite or unequal intervals by detachably connecting a plurality of groove spacing elements with a plurality of first baffle elements, so that the sizes of a plurality of membrane washing grooves are adjusted, membrane washing liquid is saved, and the membrane washing device is suitable for membrane washing space requirements with different sizes;

(4) the control unit is used for controlling the device to automatically discharge or discharge liquid at regular time, so that the material waste is reduced.

Drawings

FIG. 1 is a schematic perspective view of an automatic membrane washing device for Western blotting according to the present invention;

fig. 2 is a schematic three-dimensional structure diagram of a liquid storage unit in an automatic membrane washing device for western blotting of the present invention;

FIG. 3 is a schematic diagram of a three-dimensional structure of a shunting unit in an automatic membrane washing device for Western blotting of the present invention;

FIG. 4 is a schematic view of the three-dimensional structure of the membrane washing unit in the automatic membrane washing device for western blotting of the present invention

FIG. 5 is a schematic view of a partial cross-sectional structure inside a membrane washing unit of an automatic membrane washing device for Western blotting according to the present invention;

FIG. 6 is a schematic perspective view of a liquid discharge unit of an automatic membrane washing device for Western blotting according to the present invention;

FIG. 7 is a schematic view of a partial cross-sectional structure of a membrane washing unit and a liquid collecting unit when a liquid discharging unit is at a liquid discharging working position in an automatic membrane washing device for Western blotting of the present invention;

fig. 8 is a partial enlarged view at a of fig. 7;

fig. 9 is a schematic view of a partial structure of the membrane washing unit and the liquid collecting unit when the liquid discharging unit is located at the membrane washing working position in the automatic membrane washing device for western blotting of the present invention;

FIG. 10 is a schematic view of the partial cross-sectional configuration of FIG. 9 at B;

fig. 11 is a schematic perspective view of a liquid collecting unit in an automatic membrane washing device for western blotting according to the present invention;

FIG. 12 is a schematic structural diagram of a control unit in an automatic membrane washing device for Western blotting according to the present invention;

wherein the reference numerals are:

100. a liquid storage unit; 101. a support element; 102. a liquid storage element; 103. a first liquid inlet element; 104. a first outlet element; 105. an inlet cap element;

200. a shunting unit; 201. a first flow directing element; 202. a shunt element; 203. a second flow directing element; 204. a first clamping element; 205. a second clamping element;

300. a membrane washing unit; 301. a film washing frame element; 302. a separation groove element; 303. a first baffle element; 304. a separator element; 305. a membrane washing tank; 306. a second liquid inlet element; 307. a cover member; 308. a first drainage aperture; 309. a second drainage hole; 310. a third drainage aperture; 311. a recess element; 312. a guide member; 313. an opening member;

400. a liquid discharging unit; 401. a second baffle element; 402. a third baffle element; 403. a fourth drainage aperture; 404. a fifth drainage hole;

500. a liquid collecting unit; 501. a liquid collecting element; 502. a sixth drainage aperture; 503. a second outlet element; 504. a liquid outlet cover element;

600. a control unit; 601. a flow monitoring element; 602. a switching element; 603. a baffle control element; 604. a timing element;

700. a horizontal shaking table.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the protection scope of the present invention.

It should be noted that, in the case of no conflict, the embodiments and features of the embodiments of the present invention may be combined with each other.

The present invention will be further described with reference to the accompanying drawings and specific embodiments, but the present invention is not limited thereto.

An automatic membrane washing device for western blotting, as shown in fig. 1, comprises a liquid storage unit 100, a shunting unit 200, a membrane washing unit 300, a liquid discharging unit 400, a liquid collecting unit 500 and a control unit 600. The liquid storage unit 100 is used for storing membrane washing liquid; the shunting unit 200 is arranged at the downstream of the liquid storage unit 100 and is used for uniformly shunting the membrane washing liquid; the membrane washing unit 300 is arranged at the downstream of the shunting unit 200 and used for storing membrane washing liquid for membrane washing; the liquid discharging unit 400 is arranged at the front side of the interior of the membrane washing unit 300, and is in sliding connection with the interior of the membrane washing unit 300 for opening or closing the membrane washing unit 300. The liquid collecting unit 500 is horizontally arranged on the horizontal shaking table 700, and the outer wall of the top of the liquid collecting unit 500 is connected with the outer wall of the bottom of the membrane washing unit 300 and is used for collecting waste liquid after membrane washing; the control unit 600 is respectively connected to the liquid storage unit 100, the shunting unit 200 and the liquid discharging unit 400, and is configured to control the shunting unit 200 to deliver the membrane washing solution of the liquid storage unit 100 to the membrane washing unit 300, and control the liquid discharging unit 400 to flow the membrane washing solution of the membrane washing unit 300 out to the liquid collecting unit 500.

As shown in fig. 2, the liquid storage unit 100 includes a support member 101, a liquid storage member 102, a first liquid inlet member 103, a first liquid outlet member 104, and an inlet cover member 105. The liquid storage element 102 is horizontally arranged on the supporting element 101, and the outer wall of the bottom of the liquid storage element is connected with the outer wall of the top of the supporting element 101; the first liquid inlet element 103 is arranged at the top of one side of the liquid storage element 102; the first liquid outlet element 104 is arranged at the middle lower part of the front side of the liquid storage element 102; the liquid inlet cover element 105 is disposed above the liquid storage element 102, and detachably connected to an outer surface of the first liquid inlet element 103, for opening or closing the first liquid inlet element 103.

In some of these embodiments, the support member 101 is a table for supporting and positioning the reservoir member 102.

In some embodiments, the reservoir member 102 is a reservoir for storing the membrane-washing solution.

In some embodiments, the first inlet element 103 is an inlet, and when the amount of the membrane-washing solution stored in the reservoir element 102 is too small, the membrane-washing solution can be injected into the reservoir element 102 through the first inlet element 103.

In some of these embodiments, the first outlet element 104 is an outlet.

In some of these embodiments, the inlet cap element 105 is an inlet cap for opening or closing the first inlet element 103.

As shown in fig. 3, the flow diversion unit 200 comprises a first flow diversion element 201, a flow diversion element 202, and several second flow diversion elements 203. The first drainage element 201 is arranged at the downstream of the liquid storage unit 100, and an inlet of the first drainage element 201 is connected with the liquid storage unit 100; the flow dividing element 202 is arranged at the downstream of the first flow guiding element 201, and the inlet of the flow dividing element 202 is connected with the outlet of the first flow guiding element 201; the plurality of second drainage elements 203 are arranged at the downstream of the flow dividing element 202, the inlets of the plurality of second drainage elements 203 are connected with the outlets of the flow dividing element 202, and the outlets of the plurality of second drainage elements 203 are connected with the membrane washing unit 300.

Specifically, the first flow directing element 201 is arranged downstream of the first liquid outlet element 104, and an inlet of the first flow directing element 201 is connected with the first liquid outlet element 104.

Further, the flow dividing unit 200 further comprises a first clamping element 204 and several second clamping elements 205. The first clamping element 204 is arranged on the outer surface of the inlet of the first drainage element 201 and used for clamping and fixing the connection between the first drainage element 201 and the liquid storage unit 100; the plurality of second clamping elements 205 are respectively arranged on the outer surface of the outlet of the plurality of second drainage elements 203 and used for clamping and fixing the connection between the second drainage elements 203 and the membrane washing unit 300.

Specifically, the first clamping element 204 is disposed on the outer surface of the inlet of the first flow directing element 201, and is used for clamping and fixing the connection between the inlet of the first flow directing element 201 and the first liquid outlet element 104; the plurality of second clamping elements 205 are respectively arranged on the outer surfaces of the outlets of the plurality of second drainage elements 203 and used for clamping and fixing the connection between the outlets of the second drainage elements 203 and the membrane washing unit 300.

In some embodiments, the first drainage element 201 is a drainage tube for draining the membrane-washing solution in the solution storage unit 100 into the flow dividing element 202.

In some of these embodiments, the flow dividing element 202 is a flow divider for dividing the liquid in the first flow directing element 201 into equal portions, which then flow into the second flow directing element 203.

In some embodiments, the second drainage element 203 is a flexible drainage tube for draining the membrane-washing liquid from the interior of the flow-dividing element 202 to the membrane-washing unit 300, and the flexible design enhances the connection between the second drainage element 203 and the flow-dividing element 202 and the membrane-washing unit 300, thereby improving the stability of the device.

In some of these embodiments, the first clamping element 204 is a tube clamp, enhancing the stability of the connection between the inlet of the first flow directing element 201 and the first liquid outlet element 104.

In some of these embodiments, the second clamping element 205 is a tube clamp, enhancing the stability of the connection between the outlet of the second flow directing element 203 and the membrane washing unit 300.

As shown in fig. 4 and 5, the membrane washing unit 300 comprises a membrane washing frame element 301, a plurality of separation groove elements 302, a plurality of first baffle elements 303, a baffle element 304, a plurality of membrane washing grooves 305, a plurality of second liquid inlet elements 306, a cover element 307, a groove element 311, a guide element 312 and an opening element 313. Wherein the membrane washing frame element 301 is arranged downstream of the flow dividing unit 200; the plurality of separation groove elements 302 are symmetrically arranged on the inner wall of the rear side of the film washing frame element 301 and the middle and front side of the interior of the film washing frame element 301 at intervals; the plurality of first baffle elements 303 are vertically arranged inside the membrane washing frame element 301 at intervals, the outer walls of the bottoms of the first baffle elements 303 are connected with the inner wall of the bottom of the membrane washing frame element 301, and the outer walls of the two sides of the first baffle elements 303 are detachably connected with the inner wall of the separation groove element 302; the clapboard element 304 is embedded in the membrane washing frame element 301, the outer wall of the bottom of the clapboard element is connected with the inner wall of the membrane washing frame element 301, the outer wall of the clapboard element 304 is connected with the inner wall of the membrane washing frame element 301, and the rear side of the clapboard element 304 is connected with the outer wall of the front side of the plurality of separation groove elements 302; if the dry cleaning film tank 305 is disposed inside the film washing frame member 301, the plurality of partition groove members 302, the plurality of first baffle members 303 and the partition member 304 divide the inner space of the film washing frame member 301 into the plurality of film washing tanks 305; the second liquid inlet elements 306 are arranged on the outer wall of the middle upper part of the rear side of the membrane washing frame element 301 at intervals and are connected with the flow dividing unit 200; the cover plate element 307 is arranged at the top opening of the film washing frame element 301 and is hinged at one side of the top of the film washing frame element 301; a groove member 311 is provided at the bottom of the film washing frame member 301 for detachably disposing the partition member 304; the guide member 312 is provided on the front inner wall of the film washing frame member 301; the opening member 313 is provided at one side of the film washing frame member 301.

Specifically, the membrane washing frame member 301 is disposed downstream of the second flow guide member 203; the second liquid inlet elements 306 are arranged on the outer wall of the middle upper part of the rear side of the membrane washing frame element 301 at intervals and are connected with the outlets of the second drainage elements 203.

Further, the membrane washing unit 300 further includes a plurality of first drainage holes 308, a plurality of second drainage holes 309, and a plurality of third drainage holes 310. Wherein, the plurality of first drainage holes 308 are arranged at one side of the front side of the dry cleaning film groove 305, and the first drainage holes 308 are circular openings arranged at the middle part of one side of the horizontal bottom of the clapboard element 304; the second drainage holes 309 are formed in the front sides of the film washing grooves 305, the second drainage holes 309 and the first drainage holes 308 are formed in the same side, and the second drainage holes 309 are rectangular screening openings formed in the bottoms of the vertical side walls of the partition plate elements 304 and used for screening materials in the film washing grooves 305; a plurality of third drainage holes 310 are circular openings arranged on the bottom groove element 311 of the membrane washing frame element 301, the aperture of the third drainage holes 310 is larger than that of the first drainage holes 308, and the third drainage holes 310 are arranged under the first drainage holes 308.

In some of these embodiments, the membrane washing frame element 301 is a membrane washing tank.

In some embodiments, the separation groove elements 302 are separation grooves, and the positions of the first baffle elements 303 connected to the separation groove elements 302 are adjusted to adjust the positions of the first baffle elements 303 disposed on the membrane washing frame element 301, so as to adjust the space size of the membrane washing tank 305, adjust the space of the membrane washing tank 305 according to the membrane washing requirement, and save the membrane washing solution.

In some of the embodiments, the number of the first baffle elements 303 is 5 baffles, which divide the inner space between the membrane washing frame element 301 and the baffle element 304 into 6 membrane washing tanks 305, and the second inlet element 306 is disposed on the rear middle upper outer wall of each membrane washing tank 305. The liquid after being split by the splitting unit 200 enters the membrane washing tank 305 through the second liquid inlet element 306.

In some embodiments, the cover member 307 is a box cover for closing the top of the film washing tank 305, preventing the materials inside the film washing tank 305 from being contaminated by the outside, and preventing the materials inside the film washing tank 305 from splashing outside the device during the film washing process.

As shown in fig. 6 to 10, the tapping unit 400 includes second and third baffle members 401 and 402, fourth and fifth drainage holes 403 and 404. Wherein the second baffle member 401 is slidably disposed inside the film washing unit 300; the third baffle element 402 is vertically connected to one side of the second baffle element 401, and one side of the third baffle element 402 is connected with the outer wall of one side of the membrane washing unit 300 during waste liquid discharge, and the third baffle element 402 can vertically cover the side wall of the membrane washing unit 300; the plurality of fourth drainage holes 403 are rectangular openings arranged at the bottom of the vertical side wall of the second baffle element 401 at intervals, and the fourth drainage holes 403 are communicated with the membrane washing unit 300 when waste liquid is discharged; a plurality of fifth drainage holes 404 are rectangular openings arranged at the bottom of the second baffle element 401 at intervals, the fifth drainage holes 404 are communicated with the fourth drainage holes 403, and the fifth drainage holes 404 are communicated with the membrane washing unit 300 when waste liquid is drained.

Specifically, the second baffle element 401 is slidably connected with the guide element 312 of the film washing unit 300, the diaphragm element 304, the rear side of the rear vertical outer wall of the second baffle element 401 is slidably connected with the front side of the rear vertical outer wall of the diaphragm element 304, the bottom of the bottom horizontal outer wall of the second baffle element 401 is slidably connected with the top of the bottom horizontal outer wall of the diaphragm element 304, and the bottom horizontal outer wall of the second baffle element 401 is slidably connected with the guide element 312 of the film washing frame element 301; the third baffle element 402 can cover the opening element 313 at one side of the membrane washing frame element 301 when discharging waste liquid; the plurality of fourth drainage holes 403 are opening elements 313 arranged at the bottom of the vertical side wall of the second baffle element 401 at intervals, the size of the fourth drainage holes 403 is smaller than that of the second drainage holes 309, and the fourth drainage holes 403 are arranged right in front of the second drainage holes 309 when waste liquid is drained; the width of the fifth drainage hole 404 is greater than the diameter of the third drainage hole 310, and the fifth drainage hole 404 is disposed right above the third drainage hole 310 when discharging waste liquid.

In some of these embodiments, second baffle member 401 is an integral baffle formed of a vertical baffle and a horizontal baffle for opening or closing first and second flow-directing apertures 308, 309 provided in the sidewall of baffle member 304.

In some of these embodiments, the third baffle element 402 is a baffle for closing the opening 313 of the membrane washing frame element 301 when the membrane washing tank 305 is drained of waste liquid, preventing the waste liquid from leaking out of the device.

In some of these embodiments, the plurality of fifth drainage apertures 404 are a plurality of drainage ports. Before the film washing tank 305 is ready to inject materials for film washing, the second baffle element 401 slides to the film washing working position to close the first drainage holes 308 and the second drainage holes 309, so that the materials in the film washing tank 305 cannot be discharged. After the film washing of the film washing groove 305 is completed, the second baffle element 401 slides to the liquid discharge working position, the third baffle element 402 closes the rectangular opening on one side of the film washing frame element 301, the fourth drainage holes 403 are arranged right in front of the second drainage holes 309, and the fifth drainage holes 404 are arranged right above the third drainage holes 310; the material in the film washing groove 305 is sieved through the second drainage holes 309, effective materials are stored in the film washing groove 305, waste liquid is sieved through the second drainage holes 309 to move the fourth drainage holes 403 on the front side of the waste liquid, the fifth drainage holes 404 are moved from the fourth drainage holes 403, the third drainage holes 310 below the fifth drainage holes 404 are moved from the fifth drainage holes 404, and finally the third drainage holes 310 are moved to the liquid collecting unit 500.

As shown in fig. 11, the liquid collecting unit 500 comprises a liquid collecting element 501, a plurality of sixth drainage holes 502, a second liquid outlet element 503 and a liquid outlet cover element 504. Wherein, the liquid collecting element 501 is horizontally arranged on the horizontal shaking table 700, and the outer wall of the top of the liquid collecting element 501 is connected with the outer wall of the bottom of the membrane washing unit 300; the plurality of sixth drainage holes 502 are circular openings formed in the top of the liquid collecting element 501, and the sixth drainage holes 502 are communicated with the membrane washing unit 300; the second liquid outlet element 503 is arranged at the middle lower part of one side of the liquid collecting element 501; the outlet cover element 504 is detachably connected to the outer surface of the second outlet element 503 for opening or closing said second outlet element 503.

Specifically, the top outer wall of the liquid collecting element 501 is connected with the bottom outer wall of the membrane washing frame element 301; the aperture of the sixth drainage hole 502 is greater than that of the third drainage hole 310, and the sixth drainage hole 502 is provided right below the third drainage hole 310.

In some of these embodiments, the liquid collection element 501 is a waste liquid tank, and waste liquid moves from the third drain hole 310 to the sixth drain hole 502 directly below the waste liquid tank, and from the sixth drain hole 502 to the inside of the liquid collection element 501.

In some embodiments, the second outlet element 503 is an outlet, and when the liquid waste in the liquid collecting element 501 is excessive, the liquid waste can be discharged out of the liquid collecting element 501 through the second outlet element 503.

In some of these embodiments, the outlet cover element 504 is an outlet cover for opening or closing the second outlet element 503.

As shown in fig. 12, the control unit 600 includes a plurality of flow monitoring elements 601, a plurality of switching elements 602, a flapper control element 603, and a timing element 604. The flow monitoring elements 601 are respectively arranged at the inlet and/or the outlet of the shunting unit 200 and used for measuring the flow of the membrane washing solution of the shunting unit 200; the switch elements 602 are respectively arranged at the inlet and/or the outlet of the shunting unit 200 and are used for controlling the flow and stop of the membrane-washing solution of the shunting unit 200; the baffle control element 603 is arranged on the liquid discharging unit 400, so that the membrane-washing liquid of the membrane-washing unit 300 flows out to the liquid collecting unit 500; the timing element 604 is disposed on the liquid storage unit 100, and is connected to the switch element 602 and the baffle control element 603, respectively, for controlling the switch element 602 and the baffle control element 603.

Specifically, the flow monitoring elements 601 are respectively disposed at the inlet or the outlet of the first drainage element 201, and/or the inlet or the outlet of the second drainage elements 203, and are used for measuring the flow rate of the membrane-washing solution of the shunting unit 200; the switch elements 602 are respectively arranged at the inlet or the outlet of the first drainage element 201 and/or the inlet or the outlet of the second drainage elements 203 and are used for controlling the flow and the stop of the membrane-washing liquid of the flow-dividing unit 200; the baffle control element 603 is arranged on the third baffle element 402, so that the membrane-washing solution in the membrane-washing tank 305 flows out to the sixth drainage hole 502; the timing element 604 is disposed on the supporting element 101 for controlling the switch element 602 and the shutter controlling element 603.

In some of these embodiments, the flow monitoring component 601 is a flow meter for measuring the flow rate of the membrane-washing liquid of the flow-dividing unit 200.

In some embodiments, the switch element 602 is a water inlet solenoid valve, and under the control of the timing element 604 and the flow rate monitoring element 601, the switch element 602 controls the flow and stop of the membrane-washing solution of the flow-dividing unit 200, so as to control the injection amount of the membrane-washing solution if the membrane-washing tank 305 is dry-cleaned.

In some embodiments, the spaces of the plurality of membrane washing tanks 305 are different in size, and under the control of the timing element 604 and the plurality of flow rate monitoring elements 601 disposed at the outlets of the plurality of second flow directing elements 203, the plurality of switch elements 602 disposed at the outlets of the plurality of second flow directing elements 203 control the flow and stop of the membrane washing liquid of the flow dividing unit 200, so that the plurality of membrane washing tanks 305 are filled with different amounts of membrane washing liquid respectively.

In some embodiments, the baffle control component 603 is a solenoid valve, and under the control of the timing component 604, the baffle control component 603 controls the tapping unit 400 to slide to the film washing working position or the tapping working position.

The utility model discloses a use method as follows:

before use, the liquid inlet cover element 105 is detached, the membrane washing liquid is injected into the liquid storage element 102 through the first liquid inlet element 103, and then the first liquid inlet element 103 is closed through the liquid inlet cover element 105; the liquid outlet cover element 504 is disassembled, the waste liquid is discharged out of the liquid collecting element 501 through the second liquid outlet element 503, and the second liquid outlet element 503 is closed through the liquid outlet cover element 504; the position where the plurality of first baffle members 303 are connected to the plurality of separation groove members 302 is adjusted, thereby adjusting the size of the plurality of membrane washing tanks 305.

When the device is used, the parameters of the timing element 604 are preset according to the membrane washing requirement, the switch element 602 is started, the baffle control element 603 controls the liquid discharge unit 400 to slide to the membrane washing working position, the liquid storage unit 100 starts to inject membrane washing liquid into the shunting unit 200, the membrane washing liquid moves into the membrane washing tank 305 through the shunting unit 200, and when the liquid flow in the shunting unit 200 meets the requirement of the flow monitoring element 601, the switch element 602 closes the shunting unit 200 and stops liquid discharge.

The film washing unit 300 performs film washing operation on the horizontal shaking table 700, when the film washing time reaches the set time of the timing element 604, the baffle control element 603 controls the liquid discharge unit 400 to slide to a liquid discharge working position, materials in the film washing groove 305 are sieved through the second drainage holes 309, effective materials are stored in the film washing groove 305, waste liquid is sieved through the second drainage holes 309 and moves to the fourth drainage holes 403 on the front side of the waste liquid, moves from the fourth drainage holes 403 to the fifth drainage holes 404, moves from the fifth drainage holes 404 to the third drainage holes 310 below the waste liquid, and finally moves from the third drainage holes 310 to the sixth drainage holes 502 right below the waste liquid, and moves from the sixth drainage holes 502 to the inside of the liquid collecting element 501.

After the waste liquid is discharged, the timing element 604 controls the switch element 602 and the baffle control element 603 to perform secondary membrane washing until the device finishes the preset membrane washing times.

After use, the waste liquid in the liquid collecting element 501 is discharged, and the effective materials are taken out from the membrane washing tank 305.

The utility model has the advantages that the operation of filling the film washing liquid is simple and rapid; the membrane washing requirements with different space requirements are met; time is saved, and material waste is reduced.

The above description is only an example of the preferred embodiment of the present invention, and not intended to limit the scope of the present invention, and those skilled in the art should be able to realize the equivalent alternatives and obvious variations of the present invention.

Claims (10)

1. An automatic membrane washing device for western blotting, comprising a liquid storage unit (100), a shunting unit (200), a membrane washing unit (300), a liquid discharging unit (400), a liquid collecting unit (500) and a control unit (600), wherein:

the liquid storage unit (100) is used for storing membrane washing liquid;

the shunting unit (200) is arranged at the downstream of the liquid storage unit (100) and is used for uniformly shunting the membrane washing liquid;

the membrane washing unit (300) is arranged at the downstream of the shunting unit (200) and is used for storing the membrane washing liquid for membrane washing;

the liquid discharging unit (400) is arranged on the front side of the interior of the membrane washing unit (300), is in sliding connection with the interior of the membrane washing unit (300), and is used for opening or closing the membrane washing unit (300);

the liquid collecting unit (500) is horizontally arranged on the horizontal shaking table (700), and the outer wall of the top of the liquid collecting unit (500) is connected with the outer wall of the bottom of the membrane washing unit (300) and is used for collecting membrane washing liquid after membrane washing;

the control unit (600) respectively with stock solution unit (100), reposition of redundant personnel unit (200) with tapping unit (400) are connected, are used for control reposition of redundant personnel unit (200) will stock solution unit (100) the washing membrane liquid carry to washing membrane unit (300) and control tapping unit (400) will washing membrane unit (300) the washing membrane liquid flow extremely collecting unit (500).

2. An automatic membrane washing device for western blotting according to claim 1, wherein the reservoir unit (100) comprises a support element (101), a reservoir element (102), a first liquid inlet element (103), a first liquid outlet element (104), and an inlet cap element (105), wherein:

the liquid storage element (102) is horizontally arranged at the top of the supporting element (101), and the outer wall of the bottom of the liquid storage element is connected with the outer wall of the top of the supporting element (101);

the first liquid inlet element (103) is arranged at the top of one side of the liquid storage element (102);

the first liquid outlet element (104) is arranged at the middle lower part of the front side of the liquid storage element (102);

the liquid inlet cover element (105) is arranged above the liquid storage element (102), is detachably connected with the outer surface of the first liquid inlet element (103), and is used for opening or closing the first liquid inlet element (103).

3. An automatic membrane washing device for western blotting according to claim 1, wherein the shunting unit (200) comprises a first draining element (201), a shunting element (202) and several second draining elements (203), wherein:

the first drainage element (201) is arranged at the downstream of the liquid storage unit (100), and an inlet of the first drainage element (201) is connected with the liquid storage unit (100);

the flow dividing element (202) is arranged at the downstream of the first flow guiding element (201), and the inlet of the flow dividing element (202) is connected with the outlet of the first flow guiding element (201);

the second drainage elements (203) are arranged at the downstream of the flow dividing element (202), the inlets of the second drainage elements (203) are connected with the outlets of the flow dividing element (202), and the outlets of the second drainage elements (203) are connected with the membrane washing unit (300).

4. An automatic membrane washing device for western blotting according to claim 3, wherein the flow dividing unit (200) further comprises a first clamping element (204) and several second clamping elements (205), wherein:

the first clamping element (204) is arranged on the outer surface of the inlet of the first drainage element (201) and used for clamping and fixing the connection between the first drainage element (201) and the liquid storage unit (100);

the second clamping elements (205) are respectively arranged on the outer surfaces of the outlets of the second drainage elements (203) and used for clamping and fixing the second drainage elements (203) and the membrane washing unit (300).

5. The automatic membrane washing device for western blotting according to claim 1, wherein the membrane washing unit (300) comprises a membrane washing frame element (301), a plurality of separation groove elements (302), a plurality of first baffle elements (303), a separator element (304), a plurality of membrane washing tanks (305), and a plurality of second inlet elements (306) and a cover plate element (307), wherein:

the membrane washing frame element (301) is arranged at the downstream of the flow dividing unit (200);

the plurality of separation groove elements (302) are symmetrically arranged on the inner wall of the rear side of the membrane washing frame element (301) and the middle and front side of the interior of the membrane washing frame element (301) at intervals;

a plurality of first baffle elements (303) are vertically arranged inside the membrane washing frame element (301) at intervals, the outer walls of the bottoms of the first baffle elements (303) are connected with the inner wall of the bottom of the membrane washing frame element (301), and the outer walls of the two sides of the first baffle elements (303) are detachably connected with the inner wall of the separation groove element (302);

the membrane washing frame element (301) is embedded in the partition plate element (304), the outer wall of the bottom and the side wall of one side of the partition plate element (304) are respectively connected with the inner wall of the bottom and the inner wall of one side of the membrane washing frame element (301), and the rear side of the partition plate element (304) is connected with the outer wall of the front side of the plurality of separation groove elements (302);

the plurality of membrane washing grooves (305) are arranged inside the membrane washing frame element (301), and the internal space of the membrane washing frame element (301) is divided into the plurality of membrane washing grooves (305) by the plurality of separating groove elements (302), the plurality of first baffle elements (303) and the partition plate element (304);

the second liquid inlet elements (306) are arranged on the outer wall of the middle upper part of the rear side of the membrane washing frame element (301) at intervals and are connected with the flow dividing unit (200);

the cover plate element (307) is arranged at the top opening of the film washing frame element (301) and is hinged to one side of the top of the film washing frame element (301).

6. The automatic membrane washing device for western blotting according to claim 5, wherein the membrane washing unit (300) further comprises a plurality of first drainage holes (308), a plurality of second drainage holes (309), and a plurality of third drainage holes (310), wherein:

the first drainage holes (308) are arranged on one side of the front side of the membrane washing grooves (305), and the first drainage holes (308) are round openings arranged in the middle of one side of the horizontal bottom of the separator element (304);

the second drainage holes (309) are formed in the front sides of the film washing grooves (305), the second drainage holes (309) and the first drainage holes (308) are formed in the same side, and the second drainage holes (309) are rectangular screening openings formed in the bottoms of the vertical side walls of the partition plate elements (304) and used for screening materials in the film washing grooves (305);

a plurality of third drainage holes (310) are circular openings arranged at the bottom of the membrane washing frame element (301), the aperture of each third drainage hole (310) is larger than that of each first drainage hole (308), and the third drainage holes (310) are arranged under the first drainage holes (308).

7. An automatic membrane washing device for western blotting according to claim 1, wherein the tapping unit (400) comprises a second baffle element (401) and a third baffle element (402), wherein:

the second baffle element (401) is arranged inside the film washing unit (300) and is in sliding connection with the film washing unit (300);

the third baffle element (402) is vertically connected to one side of the second baffle element (401), and one side of the third baffle element (402) is connected with the outer wall of one side of the membrane washing unit (300) during waste liquid discharge.

8. The automatic membrane washing device for western blotting according to claim 7, wherein the liquid discharging unit (400) further comprises a plurality of fourth drainage holes (403) and a plurality of fifth drainage holes (404), wherein:

the plurality of fourth drainage holes (403) are rectangular openings which are arranged at the bottom of the vertical side wall of the second baffle element (401) at intervals, and the fourth drainage holes (403) are communicated with the membrane washing unit (300) when waste liquid is drained;

the fifth drainage holes (404) are rectangular openings which are arranged at the horizontal bottom of the second baffle element (401) at intervals, the fifth drainage holes (404) are communicated with the fourth drainage holes (403), and the fifth drainage holes (404) are communicated with the membrane washing unit (300) when waste liquid is drained.

9. The automatic membrane washing device for western blotting according to claim 1, wherein the liquid collecting unit (500) comprises a liquid collecting element (501), a plurality of sixth drainage holes (502), a second liquid outlet element (503) and a liquid outlet cover element (504), wherein:

the liquid collecting element (501) is horizontally arranged on the horizontal shaking table (700), and the outer wall of the top of the liquid collecting element (501) is connected with the outer wall of the bottom of the membrane washing unit (300);

the sixth drainage holes (502) are round openings formed in the top of the liquid collecting element (501), and the sixth drainage holes (502) are communicated with the membrane washing unit (300);

the second liquid outlet element (503) is arranged at the middle lower part of one side of the liquid collecting element (501);

the liquid outlet cover element (504) is detachably connected with the outer surface of the second liquid outlet element (503) and is used for opening or closing the second liquid outlet element (503).

10. An automatic membrane washing device for western blotting according to claim 1, wherein the control unit (600) comprises several flow monitoring elements (601), several switching elements (602), a baffle control element (603), and a timing element (604), wherein:

the flow monitoring elements (601) are respectively arranged at the inlet and/or the outlet of the shunting unit (200) and are used for metering the flow of membrane washing liquid of the shunting unit (200);

the switch elements (602) are respectively arranged at the inlet and/or the outlet of the shunting unit (200) and are used for controlling the flow and stop of the membrane washing liquid of the shunting unit (200);

the baffle control element (603) is arranged on the liquid discharging unit (400), is connected with the liquid discharging unit (400), and is used for controlling the liquid discharging unit (400) to enable the membrane washing liquid of the membrane washing unit (300) to flow out to the liquid collecting unit (500);

the timing element (604) is arranged on the liquid storage unit (100), is respectively connected with the switch elements (602) and the baffle control element (603), and is used for controlling the switch elements (602) and the baffle control element (603).

Images