CN217284595U - Operation box for freezing embryo by liquid nitrogen - Google Patents

Abstract

The utility model relates to the technical field of medical equipment, the operation box of a embryo liquid nitrogen freezing usefulness that provides has solved the technical problem that the operation box structure that is used for splendid attire liquid nitrogen when embryo freezes among the prior art is unreasonable, the operation difficulty. The utility model provides an operation box that embryo liquid nitrogen was frozen and was used, is including the box body that has the inner chamber, the open setting in upper end of inner chamber, the box body still is provided with the subassembly of drawing gas, the intracavity is provided with the minimum liquid level's of sign liquid nitrogen sign portion, the intracavity still is provided with the locator, still be provided with operating device in the box body, operating device including set up in the guide rail of inlayer with set up in the guide rail is followed the gliding slider of guide rail, the slider is provided with the centre gripping pipe. The end of the hand-held part of the freezing carrying rod is abutted against the positioner and is inserted with slight force, and the freezing carrying rod is kept horizontal in liquid nitrogen and is fixed. The operation is very convenient, the operation efficiency is improved, and the operation difficulty is reduced.

Description

Operation box for freezing embryo by liquid nitrogen

Technical Field

The utility model relates to the technical field of medical equipment, especially, relate to an operation box that embryo liquid nitrogen was frozen and was used.

Background

Many centers in reproductive medicine generally use simple and easy foam box as the operation box of splendid attire liquid nitrogen when embryo freezes, and embryo freezes specific step is: 1. and injecting liquid nitrogen into the foam box, wherein the amount of the liquid nitrogen reaches the capacity convenient for operation. 2. The foam box is placed on a clean bench. 3. The workers adopt a vitrification freezing method to complete the freezing protection of the embryos in the freezing solution 1 and the freezing solution 2 in sequence under an electron microscope. During this time, the slide bar protection sleeve was placed in liquid nitrogen, and the cryo-scaffold was placed in liquid nitrogen. 4. The worker transferred the embryos from the cryo-2 solution to the freezing position on the anterior segment of the cryo-loading rod, which was then quickly inserted into the liquid nitrogen. 5. A handheld tweezers is used for clamping the carrying rod protective sleeve, a handheld part of the freezing carrying rod is held by one hand, and the freezing carrying rod is arranged in the carrying rod protective sleeve and is tightly sleeved. 6. And (3) holding tweezers by hand or directly holding the hand-held part of the freezing carrying rod, putting the freezing carrying rod into the sleeve of the freezing bracket, and closing the sleeve. Finally, the frozen scaffolds were placed in a liquid nitrogen tank for embryo storage.

Wherein, when the operation is carried out in the step 4-6, the front section of the carrying rod with the embryo is always kept below the liquid nitrogen surface. Due to the unreasonable structure of the operation box in the prior art, the following technical problems exist in the operation of the process steps 4-6:

1. there is an obstruction to the operating line of sight. When the embryo laboratory is operated, dark light needs to be kept, the irradiation of the light to the embryo needs to be reduced, the irradiation of the light needs to be reduced when the embryo is frozen, and the reserved light is suitable for adopting a laboratory yellow light. Operating environment light is dim, and the liquid nitrogen volatilizees and forms nitrogen vapor in addition, and the refraction etc. that light takes place in the liquid nitrogen all cause the hindrance to staff's sight, and this kind of hindrance can increase the freezing probability of carrying the pole and carrying the pole anterior segment touching protective sheath open end with the freezing that is equipped with the embryo when carrying the pole protective sheath to cup joint, causes the damage to the embryo, collides the embryo even. Although the front section and the middle section of the freezing loading rod are designed with black marks, the above situation cannot be effectively avoided.

2. There is discomfort to the operator. Although the freezing operation is carried out in the superclean bench, nitrogen vapor can not be absorbed and discharged in time, and in addition, the staff must contact the liquid nitrogen closely to reduce the sight and hinder, and under this condition, the staff face can contact nitrogen vapor, causes certain stimulation to eyes, respiratory track. This discomfort is more pronounced when the freezing effort is large, and prolonged exposure to cryogenic nitrogen vapor can cause some damage to the body.

3. The freezing support is not firmly placed. The operating personnel leans on the foam case minor face with freezing support upper end, and the lower extreme touches box inner floor bottom and puts, in view of certain mobility of incasement liquid nitrogen liquid during the operation, this kind of putting insecure, and then influences operating efficiency. On the other hand, when the work load of freezing embryo is large, the arrangement of a plurality of freezing brackets is disordered and irregular, and the operation efficiency is also influenced.

4. After freezing embryo operation, need to pour the liquid nitrogen in the foam chest back into liquid nitrogen storage jar, at this moment through the slope box, make the liquid nitrogen empty from any edge of box top, the box corner is the right angled design, lacks the device of water conservancy diversion, makes the liquid nitrogen splash floor, staff shank and instep often when empting the in-process, both caused the waste, caused uncomfortable sense, even damage to staff's skin easily again.

SUMMERY OF THE UTILITY MODEL

The utility model provides a pair of operation box that embryo liquid nitrogen freezes usefulness has solved among the prior art technical problem that the operation box structure that is used for splendid attire liquid nitrogen when embryo freezes is unreasonable, the operation difficulty.

Some embodiments adopted to solve the above technical problems include:

the utility model provides an operation box that embryo liquid nitrogen freezing was used, is including the box body that has the inner chamber, the open setting in upper end of inner chamber, the box body includes inlayer and skin, the inlayer with the heat preservation has between the skin, the box body still is provided with the subassembly of bleeding, the liquid nitrogen steam of intracavity in the subassembly extraction of bleeding, the intracavity is provided with the sign portion of the minimum liquid level of sign liquid nitrogen, the intracavity still is provided with the locator, the locator is fixed in the inlayer, still be provided with operating device in the box body, operating device including set up in the guide rail of inlayer with set up in the guide rail is followed the gliding slider of guide rail, the slider is provided with the grip pipe, the guide rail is including making the grip pipe with the coaxial horizontal segment of locator makes the grip pipe stretches out the bending section of intracavity liquid nitrogen liquid level.

The box body includes inlayer, skin and heat preservation, has guaranteed the tightness, the durability and the heat preservation characteristic of box body, makes the box body be convenient for clear up simultaneously.

Through setting up sign portion at the inlayer, can conveniently control the liquid level position of liquid nitrogen when empting the liquid nitrogen to the inner chamber, avoid the liquid nitrogen liquid level of inner chamber to hang down difficult operation excessively, perhaps, the liquid level of inner chamber is too high to cause the waste.

Through setting up the subassembly of bleeding, the subassembly of bleeding can extract the liquid nitrogen steam of liquid nitrogen liquid level top in the inner chamber, does benefit to the discharge of nitrogen steam, has reduced the hindrance of nitrogen steam to the sight and to the amazing damage of low temperature of staff's eyes and respiratory track.

Through setting up the locator, at the actual operation in-process, the staff grips the freezing middle section or the handheld part of carrying the pole of putting the embryo with tweezers centre gripping, makes freezing carry the pole to keep the level, and the position of locator is discerned roughly to the eyesight, makes freezing the end of carrying the handheld part of pole to contradict the locator earlier to keep contradicting all the time, through centripetal movement changes the conflict position, can easily find the entry of locator, inserts with strength a little. The freezing bar was held horizontally in liquid nitrogen and fixed. The operation is very convenient, the operation efficiency is improved, and the operation difficulty is reduced.

Through setting up operating device, the grip tube centre gripping carries the displacement of pole protective sheath along the guide rail to reach different operation purposes. Specifically speaking, the centre gripping pipe can make the centre gripping pipe submerge below the liquid nitrogen liquid level of intracavity along pipeline displacement, or make the centre gripping pipe stretch out above the liquid nitrogen liquid level of intracavity, and this scheme can make the staff conveniently operate the carrier bar protective sheath in two positions on ground, has improved operating efficiency.

When the worker enables the clamping pipe to be located at the highest point through the operating mechanism, the clamping pipe is located above the liquid nitrogen liquid level in the inner cavity, the sliding block is located at the bending section of the guide rail at the moment, the clamping pipe is usually located about 6cm above the liquid nitrogen liquid level, and the tail end of the carrying rod protective sleeve is inserted into the clamping pipe to be fixed through tweezers or hands. And then the clamping tube is immersed into the liquid nitrogen in the inner cavity through the operating mechanism, and the sliding block is positioned at the horizontal section of the guide rail at the moment, so that the clamping tube displaces along the horizontal section to sleeve the rod-carrying protective sleeve in the clamping tube and the freezing rod-carrying in the positioner. And finally, clamping the carrying rod protective sleeve by using tweezers, separating the freezing carrying rod out of the clamping pipe, and then putting the freezing carrying rod into the freezing support cylinder.

Preferably, the operating mechanism further comprises a cross rod arranged in the horizontal direction and a vertical rod arranged in the vertical direction, the clamping pipe is installed on the cross rod, the cross rod is installed on the sliding block through the vertical rod, and a handle is arranged at the upper end of the vertical rod.

The vertical rod is mainly used for installing the handle, so that the handle can be always above the liquid nitrogen liquid level to facilitate the handheld operation of workers, the clamping pipe is arranged on the transverse rod, the transverse rod has a certain length, the clamping pipe can be conveniently supported at a target position, and the clamping pipe corresponds to the positioner to facilitate the operation.

Preferably, the handle is sleeved with a plastic sleeve, and the plastic sleeve is provided with anti-skid grains; the anti-skid lines protrude out of the plastic sleeve, or the anti-skid lines are recessed into the plastic sleeve.

The handle parcel has the plastic sheath, because plastics have lower heat-conduction coefficient, consequently, the plastic sheath on the low temperature of liquid nitrogen is difficult to conduct to the handle through the montant, and the temperature of plastic sheath can not sharply descend, avoids the low temperature of liquid nitrogen to cause the frostbite to staff's hand.

Preferably, a bushing is arranged in the clamping tube.

The bush in the centre gripping pipe can adopt rubber materials to make to increase certain resistance, be convenient for firmly fix inserting year pole protective sheath end, carry the pole protective sheath and easily operate by fixed firm back.

Preferably, the locator includes registration arm and guide tube, the guide tube is the horn mouth form, and, the guide tube with the registration arm communicates with each other, the guide tube set up in the one end of registration arm.

The locator can set up in the below 5cm department of sign portion, and the setting of guiding tube is easily the staff and is discerned the position roughly of locating tube to the distance of staff's face and interior intracavity liquid nitrogen has been prolonged, the low temperature that further reduces nitrogen vapor to staff's eyes and respiratory track stimulates the damage.

Preferably, the guide pipe and the positioning pipe are of an integrated structure, and a liner pipe is arranged in the positioning pipe.

The bushing pipe can be made of rubber materials to increase certain resistance, so that the inserted freezing rod-carrying handheld part can be firmly fixed, the operation of workers is easy, and the operation efficiency is improved.

Preferably, the steam extraction assembly comprises a shell, the shell is provided with a steam inlet and a steam outlet, a steam extractor is arranged in the shell, the steam extractor extracts the liquid nitrogen steam in the inner cavity through the steam inlet, and the steam extractor exhausts the liquid nitrogen steam extracted by the steam extractor through the steam outlet.

The steam inlet can effectively absorb nitrogen vapor above the liquid level in the inner cavity, and the obstruction of the nitrogen vapor to the sight of workers and the stimulation to the eyes and respiratory tracts of the workers are reduced.

Preferably, a handheld portion convenient to hold by hand is arranged outside the box body, a non-slip mat is arranged on the bottom face of the box body, and the non-slip mat is located on the outer side of the box body.

Through setting up handheld portion, make things convenient for the staff both hands to hold the operation box. Set up the slipmat simultaneously, increased the stability of box body on the desktop, the difficult displacement of box body on the desktop.

Preferably, the box body is provided with a nitrogen discharging pipe for discharging liquid nitrogen in the inner cavity.

Through setting up the denitrogenation pipe, be convenient for after the freezing operation finishes, pour the liquid nitrogen drainage of intracavity back to the liquid nitrogen holding vessel in, effectively avoided the liquid nitrogen to splash the loss that causes to and discomfort, the damage that the liquid nitrogen caused the human body.

Preferably, a fixing block is further arranged in the box body, a groove is formed in the fixing block, and the groove and the guide pipe are arranged oppositely.

The operation of the freezing pole of carrying when putting into a support section of thick bamboo at last is fixed convenient in the setting of recess. And the opening direction of the groove is opposite to the guide pipe, so that the continuity of operation is convenient, and the forceps can be directly inserted into the bracket barrel after clamping the freezing carrying rod.

Compared with the prior art, the utility model provides an operation box that freezing usefulness of embryo liquid nitrogen has following advantage:

1. the box body includes inlayer, skin and heat preservation, has guaranteed the tightness, the durability and the heat preservation characteristic of box body, makes the box body be convenient for clear up simultaneously.

2. Through setting up sign portion at the inlayer, can conveniently control the liquid level position of liquid nitrogen when empting the liquid nitrogen to the inner chamber, avoid the liquid nitrogen liquid level of inner chamber to hang down difficult operation excessively, perhaps, the liquid level of inner chamber is too high to cause the waste.

3. Through setting up the subassembly of bleeding, the subassembly of bleeding can extract the liquid nitrogen steam of liquid nitrogen liquid level top in the inner chamber, does benefit to the discharge of nitrogen steam, has reduced the hindrance of nitrogen steam to the sight and to the amazing damage of low temperature of staff's eyes and respiratory track.

4. Through setting up the locator, at the actual operation in-process, the staff grips with tweezers and holds the freezing middle section or the handheld part of carrying the pole of putting the embryo, makes freezing and carries the pole and keep the level, and the position of the locator is roughly discerned to the eyesight, makes freezing the end of carrying the handheld part of pole conflict to the locator to keep conflicting all the time, changes the conflict position through centripetal movement, can easily find the entry of locator, inserts with strength a little. The freezing bar was held horizontally in liquid nitrogen and fixed. The operation is very convenient, the operation efficiency is improved, and the operation difficulty is reduced.

5. Through setting up operating device, the grip tube centre gripping carries the displacement of pole protective sheath along the guide rail to reach different operation purposes. Specifically speaking, the centre gripping pipe can make the centre gripping pipe submerge below the liquid nitrogen liquid level of intracavity along pipeline displacement, or make the centre gripping pipe stretch out above the liquid nitrogen liquid level of intracavity, and this scheme can make the staff conveniently operate the carrier bar protective sheath in two positions on ground, has improved operating efficiency.

Drawings

For purposes of explanation, several embodiments of the present technology are set forth in the following figures. The following drawings are incorporated herein and constitute a part of the detailed description. In some instances, well-known structures and components are shown in block diagram form in order to avoid obscuring the concepts of the subject technology.

FIG. 1 is a schematic view of a first orientation of a cassette for liquid nitrogen freezing of embryos.

FIG. 2 is a second view of the embryo cassette for freezing with liquid nitrogen.

Fig. 3 is an enlarged view of a portion a in fig. 2.

Fig. 4 is a schematic view of the operating mechanism.

Fig. 5 is an enlarged view of fig. 4 at B.

Fig. 6 is an exploded view of the positioner.

In the figure:

1. the heat-insulation box comprises a box body, 11, an inner cavity, 12, an inner layer, 13, an outer layer, 14, a heat-insulation layer, 15, a mark part, 16, a handheld part, 17, a nitrogen discharging pipe, 18, a fixing block, 19 and a groove.

2. The steam extraction assembly 21, the shell 22 and the steam inlet.

3. Locator, 31, registration arm, 32, guide tube, 33, bushing pipe.

4. Operating mechanism, 41, guide rail, 411, horizontal segment, 412, bending segment, 42, slider, 43, clamping tube, 431, bush, 44, cross bar, 45, vertical bar, 46 and handle.

Detailed Description

The specific embodiments illustrated below are intended as descriptions of various configurations of the subject technology and are not intended to represent the only configurations in which the subject technology may be practiced. Specific embodiments include specific details for the purpose of providing a thorough understanding of the subject technology of the present invention. It will be apparent, however, to one skilled in the art that the subject technology is not limited to the specific details shown herein and may be practiced without these specific details.

Referring to fig. 1 to 6, an operation box for embryo liquid nitrogen freezing comprises a box body 1 with an inner cavity 11, the upper end of the inner cavity 11 is open, the box body 1 comprises an inner layer 12 and an outer layer 13, a heat insulation layer 14 is arranged between the inner layer 12 and the outer layer 13, the box body 1 is further provided with a steam extraction assembly 2, the steam extraction assembly 2 extracts liquid nitrogen steam in the inner cavity 11, an identification part 15 for identifying the minimum liquid level of liquid nitrogen is arranged in the inner cavity 11, a positioner 3 is further arranged in the inner cavity 11, the positioner 3 is fixed on the inner layer 12, an operation mechanism 4 is further arranged in the box body 1, the operation mechanism 4 comprises a guide rail 41 arranged on the inner layer 12 and a sliding block 42 arranged on the guide rail 41 and sliding along the guide rail 41, the sliding block 42 is provided with a clamping tube 43, the guide rail 41 comprises a horizontal section 411 for enabling the clamping tube 43 to be coaxial with the positioner 3, and enabling the clamping tube 43 to extend out of the inner cavity 11 A curved section 412 of the internal liquid nitrogen level.

The inner layer 12 and the outer layer 13 can be made of stainless steel, and the heat insulation layer 14 can be made of foam or sponge and other materials. The retainer 3 may be made of a metal material, and the retainer 3 may be welded to the inner layer 12. The guide rail 41, the slider 42, and the clamp tube 43 may be made of stainless steel.

The marking 15 may be a graduation mark provided on the inner layer 12. The logo 15 may protrude from the inner layer 12 or the logo 15 may be recessed into the inner layer 12.

The horizontal section 411 and the curved section 412 of the guide rail 41 are of an integral structure, and the horizontal section 411 and the curved section 412 are in smooth transition.

Referring to fig. 1 to 5, in one or more embodiments, the operating mechanism 4 further includes a horizontal rod 44 disposed along the horizontal direction and a vertical rod 45 disposed along the vertical direction, the clamping tube 43 is mounted on the horizontal rod 44, the horizontal rod 44 is mounted on the sliding block 42 through the vertical rod 45, and a handle 46 is disposed at an upper end of the vertical rod 45.

The clamp tube 43 may be welded to the cross bar 44 and the cross bar 44 may be an integral structure with the vertical bar 45. The slider 42 may be fixed to the vertical rod 45 in any manner. The slider 42 may also have a certain flexibility so that the slider 42 can smoothly displace between the horizontal section 411 and the curved section 412.

The contact form of the slider 42 and the guide rail 41 may be a line contact. So that the sliding block 42 can slide on the guide rail 41 more smoothly. For example, a roller or the like that contacts the guide rail 41 may be provided on the slider 42. The roller may be rotatably coupled to the slider 42.

In one or more embodiments, the handle 46 is sleeved with a plastic sleeve, which is sleeved with anti-slip threads; the anti-skid grains protrude out of the plastic sleeve, or the anti-skid grains are recessed into the plastic sleeve.

A bushing 431 is provided in the clamping tube 43. The bushing 431 may be bonded within the clamp tube 43. Alternatively, the bush 431 may be fixed in the clamp tube 43 by interference fit using elastic deformation.

Referring to fig. 6, in one or more embodiments, the positioner 3 includes a positioning tube 31 and a guide tube 32, the guide tube 32 is in a bell mouth shape, the guide tube 32 is communicated with the positioning tube 31, and the guide tube 32 is disposed at one end of the positioning tube 31. The end of the positioning tube 31 remote from the guide tube 32 may be welded to the inner layer 12.

The guide tube 32 and the positioning tube 31 are of an integrated structure, and a liner tube 33 is arranged in the positioning tube 31. Liner 33 may be cemented into place tube 31. The liner tube 33 can also be mounted in the positioning tube 31 in an interference fit manner by means of elastic deformation.

In one or more embodiments, the steam extraction assembly 2 includes a casing 21, the casing 21 is provided with a steam inlet 22 and a steam outlet, a steam extractor is disposed in the casing 21, the steam extractor extracts liquid nitrogen vapor in the inner cavity 11 through the steam inlet 22, and the steam extractor exhausts the liquid nitrogen vapor extracted by the steam extractor through the steam outlet.

The housing 21 is disposed along the longitudinal direction of the case 1, and the housing 21 may be bonded to the case 1, or the housing 21 may be mounted on the case 1 by screws. The steam inlets 22 are uniformly distributed in the housing 21 along the length direction of the housing 21. The structure of the steam extractor is not limited and can be freely selected. The steam extractor may be secured within the housing 21 by screws. The steam extractor can be powered by a dry battery.

In one or more embodiments, a handheld portion 16 convenient for holding is arranged outside the box body 1, and a non-slip pad is arranged on the bottom surface of the box body 1 and is positioned on the outer side of the box body 1. The handle portion 16 may be a plastic sheet welded to the outer layer 13. The non-slip mat may also be a plastic sheet adhered to the outer layer 13.

In one or more embodiments, the cartridge 1 is provided with a nitrogen discharge pipe 17 for discharging the liquid nitrogen in the inner chamber 11. The pipe 17 of discharging nitrogen link up inlayer 12, outer 13 and heat preservation 14, wherein, the one end of pipe 17 of discharging nitrogen with inlayer 12 parallel and level, the other end of pipe 17 of discharging nitrogen extend outer 13, and the other end of pipe 17 of discharging nitrogen can extend outer 135mm to do benefit to and utilize the pipe 17 of discharging nitrogen.

In one or more embodiments, a fixing block 18 is further disposed in the box body 1, the fixing block 18 is provided with a groove 19, and the groove 19 is disposed opposite to the guiding tube 32. The anchor block 18 may be bonded to the inner layer 12, or the anchor block 18 may be secured to the inner layer 12 in other ways.

Alternatively, the recess 19 may be provided directly in the inner layer 12.

The subject technical solution and the corresponding details of the present invention are introduced above, and it is understood that the above description is only some embodiments of the subject technical solution of the present invention, and some details can be omitted when the subject technical solution is implemented.

In addition, in some embodiments of the above utility model, there is a possibility that a plurality of embodiments may be combined to be implemented, and various combinations are not listed at length. The above embodiments can be freely combined and implemented by those skilled in the art according to the requirements in the specific implementation so as to obtain a better application experience.

In carrying out the subject matter of the present invention, those skilled in the art can obtain other details or figures according to the subject matter and figures of the present invention, and obviously these details are not departed from the subject matter and figures of the present invention, and still fall within the scope covered by the subject matter of the present invention.

Claims (10)

1. An operation box for embryo liquid nitrogen freezing is characterized in that: comprises a box body (1) with an inner cavity (11), wherein the upper end of the inner cavity (11) is arranged in an open manner, the box body (1) comprises an inner layer (12) and an outer layer (13), a heat insulation layer (14) is arranged between the inner layer (12) and the outer layer (13), the box body (1) is also provided with a steam extraction component (2), the steam extraction component (2) extracts liquid nitrogen steam in the inner cavity (11), an identification part (15) for identifying the lowest liquid level of the liquid nitrogen is arranged in the inner cavity (11), a positioner (3) is also arranged in the inner cavity (11), the positioner (3) is fixed on the inner layer (12), an operating mechanism (4) is also arranged in the box body (1), the operating mechanism (4) comprises a guide rail (41) arranged on the inner layer (12) and a sliding block (42) arranged on the guide rail (41) and sliding along the guide rail (41), the sliding block (42) is provided with a clamping pipe (43), and the guide rail (41) comprises a horizontal section (411) enabling the clamping pipe (43) to be coaxial with the positioner (3) and a bent section (412) enabling the clamping pipe (43) to extend out of the liquid level of liquid nitrogen in the inner cavity (11).

2. The manipulation kit for liquid nitrogen freezing of embryo according to claim 1, wherein: operating device (4) still include horizontal pole (44) that set up along the horizontal direction and montant (45) that set up along vertical direction, centre gripping pipe (43) install in horizontal pole (44), horizontal pole (44) pass through montant (45) install in slider (42), the upper end of montant (45) is provided with handle (46).

3. The manipulation kit for liquid nitrogen freezing of embryo according to claim 2, wherein: the handle (46) is sleeved with a plastic sleeve, and the plastic sleeve is provided with anti-skid lines; the anti-skid grains protrude out of the plastic sleeve, or the anti-skid grains are recessed into the plastic sleeve.

4. The cassette for freezing embryo with liquid nitrogen according to claim 1, 2 or 3, characterized in that: a bushing (431) is arranged in the clamping pipe (43).

5. The manipulation kit for liquid nitrogen freezing of embryo according to claim 1, wherein: the locator (3) comprises a locating pipe (31) and a guide pipe (32), the guide pipe (32) is in a horn mouth shape, the guide pipe (32) is communicated with the locating pipe (31), and the guide pipe (32) is arranged at one end of the locating pipe (31).

6. The cassette for freezing embryo with liquid nitrogen according to claim 5, characterized in that: the guide tube (32) and the positioning tube (31) are of an integrated structure, and a liner tube (33) is arranged in the positioning tube (31).

7. The manipulation kit for liquid nitrogen freezing of embryo according to claim 1, wherein: the steam extraction component (2) comprises a shell (21), wherein the shell (21) is provided with a steam inlet (22) and a steam outlet, a steam extractor is arranged in the shell (21), the steam extractor extracts liquid nitrogen steam in the inner cavity (11) through the steam inlet (22), and the steam extractor discharges the liquid nitrogen steam extracted by the steam extractor.

8. The manipulation kit for liquid nitrogen freezing of embryo according to claim 1, wherein: the portable handheld portion (16) convenient to hold is arranged outside the box body (1), the bottom surface of the box body (1) is provided with an anti-slip pad, and the anti-slip pad is located on the outer side of the box body (1).

9. The manipulation kit for liquid nitrogen freezing of embryo according to claim 1, wherein: the box body (1) is provided with a nitrogen discharging pipe (17) for discharging liquid nitrogen in the inner cavity (11).

10. The operation box for embryo liquid nitrogen freezing according to claim 6, characterized in that: the box body (1) is also internally provided with a fixed block (18), the fixed block (18) is provided with a groove (19), and the groove (19) and the guide pipe (32) are oppositely arranged.

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