CN217237677U

CN217237677U - Novel flow cytometer for cell sorting

Info

Publication number: CN217237677U
Application number: CN202220856734.XU
Authority: CN
Inventors: 李黎明; 蒋明军; 周启艳; 陶正红; 周武庆
Original assignee: Institute of Dermatology and Skin Disease Hospital of CAMS
Current assignee: Institute of Dermatology and Skin Disease Hospital of CAMS
Priority date: 2022-04-13
Filing date: 2022-04-13
Publication date: 2022-08-19
Anticipated expiration: 2032-04-13

Abstract

The utility model discloses a novel flow cytometer of cell separation, including cell appearance analysis main part and cell appearance sorting base, the positive edge equidistance of cell appearance analysis main part is fixed to be equipped with a plurality of connecting seats, and the jack has all been seted up to the one end of a plurality of connecting seats, and the inside interlude of jack is connected with the plug, and the one end fixedly connected with data line of plug is provided with block mechanism between plug and the connecting seat, and the standing groove has been seted up on the top of cell appearance sorting base, and the inside of standing groove is inlayed and is equipped with the sorting cylinder, the top of sorting cylinder and the one end fixed connection of data line. The utility model discloses a connecting rod that standing groove inner wall both sides were equipped with is connected with the arc fixed block through the connecting rod, and the flexible of the extrusion spring control arc fixed block of establishing is overlapped to the surface of connecting rod, and is convenient for fix the separation tube through the flexible of arc fixed block, ensures the stability at the in-process separation tube that the cell was selected separately, avoids the separation tube to rock, influences the sorting of cell.

Description

Novel flow cytometer for cell sorting

Technical Field

The utility model relates to a flow cytometer, in particular to novel flow cytometer for cell separation, which belongs to the technical field of cytometers.

Background

Flow cytometers are devices that automatically analyze and sort cells. It can quickly measure, store and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in liquid, and can select specified cell subsets according to preselected parameter ranges. Most flow cytometers are zero resolution instruments that can only measure an index such as total nucleic acid, total protein, etc. of a cell, but cannot identify and measure the amount of nucleic acid or protein at a particular location. That is, its detail resolution is zero.

Present flow cytometer is at the in-process of work, and the sorting barrel is generally placed in the inside that the base was selected separately to the cytometer, does not fix, and when the sorting barrel took place to rock at the in-process of selecting separately, the sorting of influence cell easily, consequently need design a novel flow cytometer that cell was selected separately.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a novel flow cytometer of cell separation to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above purpose, the utility model provides a following technical scheme: the cell analyzer comprises a cell analyzer analysis main body and a cell analyzer sorting base, wherein a plurality of connecting seats are fixedly arranged at the front edge of the cell analyzer analysis main body at equal intervals, one ends of the connecting seats are provided with jacks, plugs are connected inside the jacks in an inserting manner, one ends of the plugs are fixedly connected with data lines, a clamping mechanism is arranged between the plugs and the connecting seats, a placing groove is arranged at the top end of the cell analyzer sorting base, a sorting barrel is embedded inside the placing groove, the top end of the sorting barrel is fixedly connected with one end of the data line, grooves are respectively arranged at two sides of the inner wall of the placing groove, limiting plates are embedded inside the two grooves, connecting rods are respectively and fixedly arranged at one sides of the two limiting plates, an arc-shaped fixed block is fixedly arranged at one end of each connecting rod after passing through the groove, and an extrusion spring is arranged on the surface of each connecting rod in a sleeved manner, one end of the extrusion spring is in contact connection with one side of the arc-shaped fixing block, and the other end of the extrusion spring is in contact connection with the inner wall of the placing groove.

As a preferred technical scheme of the utility model, block mechanism includes two L type fixture blocks and a plurality of reset spring, the cavity has been seted up to the inside of plug, the both sides of cavity all alternate and are connected with L type fixture block, the draw-in groove has all been seted up, two to the both sides of jack inner wall the one end and the corresponding draw-in groove block of L type fixture block are connected, two fixed mounting has reset spring between the L type fixture block.

As a preferred technical scheme of the utility model, the top of cavity is rotated and is connected with the knob, the one end of knob extends to the inside and fixed mounting of cavity and has the rolling wheel, the surface winding of rolling wheel is connected with the stay cord, the solid fixed ring of the equal fixedly connected with in both ends of stay cord, gu fixed ring and the one side fixed connection who corresponds L type fixture block.

As a preferred technical scheme of the utility model, two one side of arc fixed block is all fixed the subsides and is equipped with the rubber pad, two evenly distributed's anti-skidding line is seted up on the surface of rubber pad.

As a preferred technical scheme of the utility model, the fixed ejector pad that is equipped with in one side on connecting rod top, logical groove has been seted up on the top of recess, ejector pad and logical groove sliding connection.

As an optimal technical scheme of the utility model, the equal fixed mounting in four edges department of cytometer analysis main part bottom has the supporting leg, four the bottom of supporting leg is all fixed and is equipped with the slipmat.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the utility model relates to a novel flow cytometer of cell separation, the connecting rod that is equipped with through standing groove inner wall both sides is connected with the arc fixed block through the connecting rod, and the flexible of extrusion spring control arc fixed block is established to the surface cover of connecting rod, and flexible through the arc fixed block is convenient for fix the separation tube, ensures the stability at the in-process separation tube that the cell was selected separately, avoids the separation tube to rock, influences the sorting of cell.

2. The utility model relates to a novel flow cytometer of cell separation inlays through L type fixture block and draw-in groove between plug and the socket and establishes and fix, guarantees the fastness of connecting, and when plug and socket separation, the rotation through the knob drives the rotation of rolling wheel, and rotation through the rolling wheel drives receiving and releasing of stay cord, through receiving and releasing of stay cord make L type fixture block and draw-in groove separation, makes things convenient for being connected of cytometer analysis main part and sorting cylinder.

Drawings

Fig. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of the structure of the cell sorting base of the present invention;

fig. 3 is a schematic view of the connection structure of the connection seat and the plug of the present invention.

In the figure: 1. a cytometer analysis body; 2. a cell instrument sorting base; 3. a separation cylinder; 4. a data line; 5. a connecting seat; 6. a placement groove; 7. a push block; 8. an arc-shaped fixed block; 9. a connecting rod; 10. a compression spring; 11. a groove; 12. a limiting plate; 13. a jack; 14. a card slot; 15. an L-shaped fixture block; 16. a cavity; 17. a return spring; 18. a fixing ring; 19. a knob; 20. a winding wheel; 22. and pulling a rope.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-3, the present invention provides a technical solution of a novel flow cytometer for cell sorting:

as shown in the figures 1-3, the cell analyzer comprises a cell analyzer main body 1 and a cell analyzer sorting base 2, a plurality of connecting seats 5 are fixedly arranged at the front edge of the cell analyzer main body 1 in an equidistant manner, one ends of the connecting seats 5 are respectively provided with an insertion hole 13, a plug is inserted and connected in the insertion hole 13, one end of the plug is fixedly connected with a data line 4, a clamping mechanism is arranged between the plug and the connecting seats 5, the top end of the cell analyzer sorting base 2 is provided with a placing groove 6, a sorting barrel 3 is embedded in the placing groove 6, the top end of the sorting barrel 3 is fixedly connected with one end of the data line 4, two sides of the inner wall of the placing groove 6 are respectively provided with a groove 11, two limiting plates 12 are embedded in the two grooves 11, one side of each of the two limiting plates 12 is fixedly provided with a connecting rod 9, one end of each connecting rod 9 is fixedly provided with an arc-shaped fixing block 8 by penetrating through the groove 11, the surface of the connecting rod 9 is sleeved and connected with an extrusion spring 10, one end of the extrusion spring 10 is in contact with one side of the arc-shaped fixed block 8, the other end of the extrusion spring 10 is in contact with the inner wall of the placing groove 6, the arc-shaped fixed block 8 is extruded through the extrusion spring 10 to fix the sorting barrel 3, and the stability of the sorting barrel 3 in the sorting process is guaranteed.

According to fig. 1 and 3, the clamping mechanism comprises two L-shaped fixture blocks 15 and a plurality of reset springs 17, a cavity 16 is formed in the plug, the L-shaped fixture blocks 15 are connected to two sides of the cavity 16 in an inserted mode, clamping grooves 14 are formed in two sides of the inner wall of the insertion hole 13, one ends of the two L-shaped fixture blocks 15 are connected with the corresponding clamping grooves 14 in a clamped mode, the reset springs 17 are fixedly mounted between the two L-shaped fixture blocks 15, a knob 19 is rotatably connected to the top of the cavity 16, one end of the knob 19 extends to the inside of the cavity 16 and is fixedly provided with a winding wheel 20, a pull rope 22 is connected to the surface winding of the winding wheel 20, fixing rings 18 are fixedly connected to two ends of the pull rope 22, the fixing rings 18 are fixedly connected with one sides of the corresponding L-shaped fixture blocks 15, the L-shaped fixture blocks 15 are clamped with the clamping grooves 14, and firmness of connection between the connection seat 5 and the plug is guaranteed.

According to fig. 1 and 2, one side of two arc fixed blocks 8 is all fixed to be pasted and is equipped with the rubber pad, evenly distributed's anti-skidding line is seted up on the surface of two rubber pads, one side on connecting rod 9 top is fixed and is equipped with ejector pad 7, logical groove has been seted up on the top of recess 11, ejector pad 7 and logical groove sliding connection, the equal fixed mounting in four edges of 1 bottom of cytometer analysis main part has the supporting leg, the bottom of four supporting legs is all fixed and is equipped with the slipmat, guarantee the stability of cytometer analysis main part 1.

When the novel flow cytometer for cell sorting is used, firstly, the push block 7 is manually pushed, the connecting rod 9 is moved to the inside of the groove 11 by the thrust of the push block 7, then, the sorting cylinder 3 is manually embedded and arranged to the inside of the placing groove 6, then, the push block 7 is manually released, at the moment, the arc-shaped fixed block 8 is moved forwards by the extrusion force of the extrusion spring 10, the sorting cylinder 3 is fixed by the arc-shaped fixed block 8, the stability of the sorting cylinder 3 in the sorting process is ensured, then, the plug of the data line 4 at the top end of the sorting cylinder 3 is inserted into the jack 13, then, the knob 19 is manually rotated, the winding wheel 20 is driven to rotate by the rotation of the knob 19, the pull rope 22 is unfolded by the winding wheel 20, at the moment, the thrust of the reset springs 17 of the two L-shaped clamping blocks 15 is clamped with the clamping groove 14, the connection of the connecting socket 5 and the plug is completed.

In the description of the present invention, it should be understood that the indicated orientation or positional relationship is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of the description, and does not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the invention.

In the present invention, unless otherwise explicitly specified or limited, for example, it may be fixedly connected, detachably connected, or integrated; can be mechanically or electrically connected; they may be directly connected or indirectly connected through an intermediate medium, and may be connected through the inside of two elements or in an interaction relationship between two elements, unless otherwise specifically defined, and the specific meaning of the above terms in the present invention will be understood by those skilled in the art according to specific situations.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A novel flow cytometer for cell sorting comprises a cytometer analysis main body (1) and a cytometer sorting base (2) and is characterized in that a plurality of connecting seats (5) are fixedly arranged at the front edge of the cytometer analysis main body (1) at equal intervals, a plurality of jacks (13) are formed in one ends of the connecting seats (5), plugs are connected inside the jacks (13) in an inserting mode, one ends of the plugs are fixedly connected with data lines (4), a clamping mechanism is arranged between the plugs and the connecting seats (5), a placing groove (6) is formed in the top end of the cytometer sorting base (2), a sorting cylinder (3) is embedded inside the placing groove (6), the top end of the sorting cylinder (3) is fixedly connected with one end of the data lines (4), grooves (11) are formed in the two sides of the inner wall of the placing groove (6), and limiting plates (12) are embedded inside the two grooves (11), two the equal fixed mounting in one side of limiting plate (12) has connecting rod (9), two the one end of connecting rod (9) is passed recess (11) fixed mounting and is had arc fixed block (8), the surface cover of connecting rod (9) is established and is connected with extrusion spring (10), the one end of extrusion spring (10) is connected with one side contact of arc fixed block (8), the other end of extrusion spring (10) is connected with the inner wall contact of standing groove (6).

2. The novel cell-sorting flow cytometer of claim 1, wherein: clamping mechanism includes two L type fixture blocks (15) and a plurality of reset spring (17), cavity (16) have been seted up to the inside of plug, the both sides of cavity (16) all alternate and are connected with L type fixture block (15), draw-in groove (14) have all been seted up to the both sides of jack (13) inner wall, two the one end of L type fixture block (15) is connected with corresponding draw-in groove (14) block, two fixed mounting has reset spring (17) between L type fixture block (15).

3. A novel cell-sorting flow cytometer as described in claim 2, wherein: the top of cavity (16) is rotated and is connected with knob (19), the one end of knob (19) extends to inside and fixed mounting of cavity (16) and takes up reel (20), the surface winding of taking up reel (20) is connected with stay cord (22), the solid fixed ring of the equal fixedly connected with in both ends (18) of stay cord (22), gu fixed ring (18) and the one side fixed connection who corresponds L type fixture block (15).

4. The novel cell-sorting flow cytometer of claim 1, wherein: rubber pads are fixedly attached to one sides of the two arc-shaped fixing blocks (8), and anti-skidding lines which are uniformly distributed are formed in the surfaces of the two rubber pads.

5. The novel cell-sorting flow cytometer of claim 1, wherein: one side of the top end of the connecting rod (9) is fixedly provided with a push block (7), the top end of the groove (11) is provided with a through groove, and the push block (7) is connected with the through groove in a sliding manner.

6. The novel cell-sorting flow cytometer of claim 1, wherein: four corners of the bottom of the main cell analyzer analysis body (1) are fixedly provided with supporting legs, and the bottom of each supporting leg is fixedly provided with an anti-slip pad.