CN217230698U - Culture medium emptying structure of fixed bed bioreactor - Google Patents
Culture medium emptying structure of fixed bed bioreactor Download PDFInfo
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- CN217230698U CN217230698U CN202220294583.3U CN202220294583U CN217230698U CN 217230698 U CN217230698 U CN 217230698U CN 202220294583 U CN202220294583 U CN 202220294583U CN 217230698 U CN217230698 U CN 217230698U
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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Abstract
The utility model discloses a fixed bed bioreactor culture medium evacuation structure belongs to fixed bed bioreactor technical field. The stirring tank comprises a tank body, an upper cover, a magnetic stirrer, a spoiler, a stirring shaft and a straight-through pipe; the stirring shaft is a step shaft, the lower part of the stirring shaft with the large diameter is fixed at the bottom of the tank body, an inner hole is formed in the axial line of the stirring shaft, a plurality of vertical emptying grooves are formed in the peripheral hole wall of the inner hole, the inner hole and the emptying grooves are formed from the top of the stirring shaft to the bottom surface of the tank body, and a magnetic stirrer is sleeved at the upper part of the stirring shaft; the upper part of the straight-through pipe is provided with a limiting ring, and the lower end of the straight-through pipe sequentially penetrates through the upper cover and the spoiler provided with the hollow hole and is inserted into the inner hole of the stirring shaft. The straight-through pipe is matched with the stirring shaft, and liquid is sucked by utilizing the emptying groove on the stirring shaft and the emptying gap below the straight-through pipe, so that the liquid can be discharged and emptied under the conditions that the liquid flow in the tank body is not interfered and the normal operation of the magnetic stirrer is not influenced.
Description
Technical Field
The utility model relates to a fixed bed bioreactor culture medium evacuation structure belongs to fixed bed bioreactor technical field.
Background
The fixed bed bioreactor mainly adopts an internal circulation mode, and the culture medium is driven by the stirring paddle to circularly stir in the tank. The stirring mode of the reactor mainly comprises two modes of ordinary stirring and magnetic stirring, wherein the magnetic stirring generally adopts a magnetic stirrer, and the magnetic stirrer drives a magnetic stirrer to do circular motion by utilizing the principle that like poles of magnetic substances in a magnetic field repel each other and opposite poles attract each other, so that liquid in the reactor is driven to rotate until the stirring is uniform. The middle part of the reactor is provided with a spoiler, which is mainly used for eliminating vortexes, improving the circulation of the main body, increasing the turbulence degree and improving the stirring effect. At present, a magnetic stirrer is generally arranged at the bottom of a fixed bed bioreactor tank body, so that an emptying port is inconvenient to arrange at the bottom of the bioreactor tank body, and therefore a suction pipeline is required to be inserted from the upper part and extend into the bottom of the bioreactor tank body to extract liquid for emptying. Due to the blockage of the culture bed, the spoiler and the magnetic stirrer arranged in the reactor tank body, the emptying pipeline cannot go deep into the bottom of the reactor tank body, and the liquid in the reactor tank body cannot be emptied completely, so that a large amount of culture medium is wasted.
Therefore, the culture medium emptying structure of the fixed bed bioreactor is designed, can be extended into the bottom of the reactor tank body, can discharge more culture mediums in the reactor tank body, reduces the waste of the culture mediums as much as possible, and does not influence the normal work of the magnetic stirrer.
Disclosure of Invention
The utility model aims to solve the technical problem that: the utility model provides a fixed bed bioreactor culture medium evacuation structure, which solves the problem that the liquid in the reactor tank body is not evacuated thoroughly by the existing fixed bed bioreactor adopting a magnetic stirring mode.
The utility model discloses the technical problem that will solve takes following technical scheme to realize:
a culture medium emptying structure of a fixed bed bioreactor comprises a tank body, an upper cover, a magnetic stirrer and a spoiler, wherein the top of the tank body is sealed by the upper cover, the bottom of the tank body is provided with the magnetic stirrer, the middle part of the tank body is provided with the spoiler, the fixed bed bioreactor also comprises a stirring shaft and a straight-through pipe;
the stirring shaft is a step shaft, the lower part of the stirring shaft with a large diameter is fixed at the bottom of the tank body, an inner hole is formed on the central axis of the stirring shaft, a plurality of vertical emptying grooves are formed in the wall of the peripheral hole of the inner hole, the inner hole and the emptying grooves are formed from the top of the stirring shaft to the bottom surface of the tank body, and a magnetic stirrer is sleeved on the upper part of the stirring shaft;
the upper part of the straight-through pipe is provided with a limiting ring, the lower end of the straight-through pipe sequentially penetrates through the upper cover and the spoiler provided with the hollow hole and is inserted into the inner hole of the stirring shaft, the limiting ring of the straight-through pipe is fixed on the upper cover, and an emptying gap is reserved between the lower end of the straight-through pipe and the bottom of the inner hole. The upper port of the straight-through pipe is connected with a peristaltic pump through a hose, the peristaltic pump is started to form negative pressure, and liquid in the tank body enters the straight-through pipe from an emptying groove of the stirring shaft and an emptying gap below the straight-through pipe and is sucked and discharged by the peristaltic pump.
As a preferable example, the magnetic stirrer consists of a plurality of blades, a rotating bracket and magnets, the blades are uniformly distributed on the same circumference of the rotating bracket, a shaft hole penetrating through the rotating bracket is arranged in the middle of the rotating bracket, a shaft sleeve is arranged in the shaft hole, the magnetic stirrer is movably arranged on the upper part of the stirring shaft through the shaft sleeve, and the magnets uniformly distributed are embedded in the bottom of the rotating bracket. A magnetic stirring base is arranged below the tank body, and the magnetic stirrer is driven to do circular motion by periodic change of polarities at two ends of the magnetic stirring base by utilizing the principle that like poles repel each other and opposite poles attract each other in a magnetic field.
As a preferable example, the inner cylinder is coaxially fixed at the bottom of the tank body, the spoiler is fixed at the upper part of the inner cylinder, and the side surface of the bottom of the inner cylinder is provided with the backflow hole.
As a preferable example, the spoiler is composed of an annular plate and a cross plate which are positioned on the same axis, four end parts of the cross plate are fixed above the annular plate, a hollow hole is formed in the middle of the cross plate, and the inner diameter of the hollow hole is larger than the outer diameter of the straight-through pipe.
As a preferred example, the evacuation gap is 1-2 mm.
The utility model has the advantages that: the straight pipe is matched with the stirring shaft, liquid is sucked by utilizing the emptying groove on the stirring shaft and the emptying gap below the straight pipe, the liquid can be discharged and emptied under the conditions that the liquid flow in the tank body is not interfered and the normal operation of the magnetic stirrer is not influenced, and when the liquid needs to be emptied, the straight pipe reaches the bottommost part, so that the liquid is more thoroughly emptied.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
FIG. 2 is a schematic top view of the bottom of the can body.
FIG. 3 is a schematic view of the magnetic stirrer assembly.
Fig. 4 is a schematic structural view of the straight-through pipe.
Fig. 5 is a schematic structural view of the spoiler.
In the figure: 1. a tank body; 2. an upper cover; 3. a magnetic stirrer; 301. a blade; 302. rotating the bracket; 303. a shaft hole; 4. a spoiler; 401. a hollow bore; 402. an annular plate; 403. a cross plate; 5. a stirring shaft; 501. an inner bore; 502. an evacuation tank; 6. a straight-through pipe; 601. a limiting ring; 7. a shaft sleeve; 8. an inner barrel; 801. and (4) a backflow hole.
Detailed Description
In order to make the technical means, the creation features, the achievement purposes and the functions of the present invention easy to understand and understand, the present invention is further explained by combining the following specific drawings.
Example 1
As shown in fig. 1-5, a culture medium emptying structure of a fixed bed bioreactor comprises a tank body 1, an upper cover 2, a magnetic stirrer 3 and a spoiler 4, wherein the top of the tank body 1 is sealed by the upper cover 2, the magnetic stirrer 3 is arranged at the bottom of the tank body 1, the spoiler 4 is arranged in the middle of the tank body 1, and the culture medium emptying structure further comprises a stirring shaft 5 and a straight-through pipe 6;
the stirring shaft 5 is a stepped shaft, the lower part of the stirring shaft 5 with the large diameter is fixed at the bottom of the tank body 1, an inner hole 501 is formed in the axial line of the stirring shaft 5, a plurality of vertical emptying grooves 502 are formed in the peripheral hole wall of the inner hole 501, the inner hole 501 and the emptying grooves 502 are formed from the top of the stirring shaft 5 to the bottom surface of the tank body 1, and a magnetic stirrer 3 is sleeved on the upper part of the stirring shaft 5;
the upper part of the straight-through pipe 6 is provided with a limiting ring 601, the lower end of the straight-through pipe 6 sequentially penetrates through the upper cover 2 and the spoiler 4 provided with the hollow hole 401 and is inserted into the inner hole 501 of the stirring shaft 5, the limiting ring 601 of the straight-through pipe 6 is fixed on the upper cover 2, and an emptying gap is reserved between the lower end of the straight-through pipe 6 and the bottom of the inner hole 501. The evacuation gap is 1-2 mm.
The working principle is as follows:
the upper port of the straight-through pipe 6 is connected with a peristaltic pump through a hose, the peristaltic pump is started to form negative pressure, and liquid in the tank body 1 enters the straight-through pipe 6 from the emptying groove 502 of the stirring shaft 5 and the emptying gap below the straight-through pipe 6 and is sucked and discharged by the peristaltic pump. When the air needs to be completely exhausted, the peristaltic pump is continuously started until the straight-through pipe 6 cannot suck the liquid, and only little liquid remains in the tank body 1 at the moment. The liquid can be discharged and emptied without interfering the liquid flow in the tank body 1 and affecting the normal operation of the magnetic stirrer 3.
Example 2
As shown in fig. 1 and fig. 3, the magnetic stirrer 3 is composed of a plurality of blades 301, a rotating bracket 302 and magnets (which are hidden inside and not shown in the figure), the plurality of blades 301 are uniformly distributed on the same circumference of the rotating bracket 302, a through shaft hole 303 is arranged in the middle of the rotating bracket 302, a shaft sleeve 7 is arranged in the shaft hole 303, the magnetic stirrer 3 is movably mounted on the upper part of the stirring shaft 5 through the shaft sleeve 7, and the plurality of uniformly distributed magnets are embedded in the bottom of the rotating bracket 302. A magnetic stirring base is arranged below the tank body 1, and the magnetic stirrer 3 is driven to do circular motion by periodic change of polarities at two ends of the magnetic stirring base by utilizing the principle that like poles repel each other and opposite poles attract each other in a magnetic field. The blades 301 are inclined blades and/or vertical blades which are sprayed upwards, the vertical blades play a role in stirring, and the inclined blades play a role in internal circulation stirring. The other structure is the same as embodiment 1.
The magnetic stirring rod 3 is sleeved on the upper part of the step of the stirring shaft 5 through the shaft sleeve 7 and can rotate freely, and the stirring function is realized under the driving of the magnetic stirring base.
Example 3
As shown in fig. 1 and 5, an inner cylinder 8 is coaxially fixed at the bottom of the tank body 1, a spoiler 4 is fixed at the upper part of the inner cylinder 8, and a reflux hole 801 is arranged on the side surface of the bottom of the inner cylinder 8. The spoiler 4 is composed of an annular plate 402 and a cross plate 403 which are positioned on the same axis, four end parts of the cross plate 403 are fixed above the annular plate 402, a hollow hole 401 is arranged in the middle of the cross plate 403, and the inner diameter of the hollow hole 401 is larger than the outer diameter of the straight-through pipe 6. The other structure is the same as in embodiment 1 or 2.
After the liquid is stirred by the magnetic stirrer 3, the liquid passes through the spoiler 4 above, main body circulation is formed on the inner side and the outer side of the inner cylinder 8, and the inner cylinder 8 and the spoiler 4 can eliminate vortex, improve the main body circulation, increase the turbulence degree and improve the stirring effect.
This structure has following advantage: the straight-through pipe 6 is matched with the stirring shaft 5, liquid is sucked by utilizing the emptying groove 502 on the stirring shaft 5 and the emptying gap below the straight-through pipe 6, the liquid can be discharged and emptied under the conditions that the liquid flow in the tank body 1 is not interfered and the normal operation of the magnetic stirrer 3 is not influenced, and when the liquid needs to be emptied, the straight-through pipe 6 reaches the bottommost part, so that the liquid is more thoroughly emptied.
The foregoing illustrates and describes the general principles, features and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the above-described embodiments, and that various changes and modifications may be made without departing from the spirit and scope of the invention, and such changes and modifications are intended to be included within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (5)
1. A culture medium emptying structure of a fixed bed bioreactor comprises a tank body, an upper cover, a magnetic stirrer and a spoiler, wherein the top of the tank body is sealed by the upper cover, the magnetic stirrer is arranged at the bottom of the tank body, and the spoiler is arranged in the middle of the tank body;
the stirring shaft is a step shaft, the lower part of the stirring shaft with the large diameter is fixed at the bottom of the tank body, an inner hole is formed in the axial line of the stirring shaft, a plurality of vertical emptying grooves are formed in the peripheral hole wall of the inner hole, the inner hole and the emptying grooves are formed from the top of the stirring shaft to the bottom surface of the tank body, and a magnetic stirrer is sleeved at the upper part of the stirring shaft;
the upper part of the straight-through pipe is provided with a limiting ring, the lower end of the straight-through pipe sequentially penetrates through the upper cover and the spoiler provided with the hollow hole and is inserted into the inner hole of the stirring shaft, the limiting ring of the straight-through pipe is fixed on the upper cover, and an emptying gap is reserved between the lower end of the straight-through pipe and the bottom of the inner hole.
2. The culture medium emptying structure of the fixed bed bioreactor as claimed in claim 1, wherein the magnetic stirrer is composed of a plurality of blades, a rotating bracket and magnets, the plurality of blades are uniformly distributed on the same circumference of the rotating bracket, a through shaft hole is arranged in the middle of the rotating bracket, a shaft sleeve is arranged in the shaft hole, the magnetic stirrer is movably arranged on the upper part of the stirring shaft through the shaft sleeve, and the plurality of magnets uniformly distributed are embedded in the bottom of the rotating bracket.
3. The medium emptying structure of a fixed bed bioreactor as claimed in claim 1, wherein the inner cylinder is coaxially fixed at the bottom of the tank body, the spoiler is fixed at the upper part of the inner cylinder, and the side surface of the bottom of the inner cylinder is provided with a backflow hole.
4. The medium evacuation structure for a fixed bed bioreactor according to claim 1, wherein the spoiler comprises a ring plate and a cross plate located on the same axis, the four ends of the cross plate are fixed above the ring plate, the center of the cross plate is provided with a hollow hole, and the through pipe passes through the hollow hole.
5. The fixed bed bioreactor media evacuation structure of claim 1, wherein the evacuation gap is 1-2 mm.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202220294583.3U CN217230698U (en) | 2022-02-11 | 2022-02-11 | Culture medium emptying structure of fixed bed bioreactor |
Applications Claiming Priority (1)
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CN202220294583.3U CN217230698U (en) | 2022-02-11 | 2022-02-11 | Culture medium emptying structure of fixed bed bioreactor |
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CN217230698U true CN217230698U (en) | 2022-08-19 |
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CN202220294583.3U Active CN217230698U (en) | 2022-02-11 | 2022-02-11 | Culture medium emptying structure of fixed bed bioreactor |
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- 2022-02-11 CN CN202220294583.3U patent/CN217230698U/en active Active
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