CN217180697U - Portable liquid electrophoresis light splitting detection device - Google Patents
Portable liquid electrophoresis light splitting detection device Download PDFInfo
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- CN217180697U CN217180697U CN202220904715.XU CN202220904715U CN217180697U CN 217180697 U CN217180697 U CN 217180697U CN 202220904715 U CN202220904715 U CN 202220904715U CN 217180697 U CN217180697 U CN 217180697U
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Abstract
The utility model discloses a portable liquid electrophoresis beam split detection device, including the sampling syringe needle, negative pressure extraction mechanism, the sampling syringe needle is connected through turn shape miniflow way in the middle of with negative pressure extraction mechanism, turn shape miniflow way turn department is provided with half anti-semi-permeable membrane, the half anti-semi-permeable membrane outside is provided with photoelectric sensor, photoelectric sensor is connected with external detection analytic system electricity, the electrophoresis is to leading-in miniflow way's liquid (solution, turbid liquid, animal and plant body fluid etc.) the change of dynamic tracking runner in the transmission spectrum of liquid when separating, and then detect specific composition and nature in the liquid, realize continuous dynamic detection and on-the-spot real-time analysis, the initial characteristic that photoelectric sensor recorded, middle process characteristic and final characteristic are analyzed, obtain the accurate expression of the eigenvalue of each particle (group) in the liquid sample; the device has small volume and can be widely used for rapid analysis and accurate detection of various liquids.
Description
Technical Field
The utility model relates to a material detection and analysis technical field, more specifically the utility model relates to a portable liquid electrophoresis beam split detection device.
Background
The electrophoresis phenomenon is a phenomenon that particles in liquid move directionally to a cathode or an anode in the liquid under the action of an external direct current power supply, and the electrophoresis detection method is widely applied to the fields of biology, pharmacology, biochemistry, medical treatment and the like and is one of the most common detection means in modern science.
The traditional electrophoresis detection method and the spectrophotometry are mutually independent test methods, and have the following defects: 1. only the final static result is detected and displayed, the characteristics in the electrophoresis dynamic process cannot be analyzed, particles with different properties can form the same electrophoresis band, and the light results cannot be distinguished when different substances (ions, colloidal particles, molecular groups and the like) in a detected sample (solution or suspension) are in a stable mixed state; 2. the detection equipment is large in size, high in cost and difficult to carry, is greatly limited to the field scientific research and real-time analysis, can be used only in a laboratory, and can not be stored for a long time after a plurality of samples are collected (especially biological samples from animals and plants) or the characters of the samples are changed after the samples are stored, so that the detection effect is poor and the cost is high; 3. traditional spectroscopic detection is only directed to homogeneous liquids, and in the electrophoresis process, the arrangement of particles in the liquid is rearranged according to the position of the electrophoretic electrode, and the process is carried out dynamically, so that different spectroscopic characteristics are presented at different positions of the liquid, and the time and space characteristics of the characteristics reflect the properties of particle groups in the liquid.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to the aforesaid not enough, provide a portable liquid electrophoresis beam split detection device to only static result, bulky, only to technical problem such as homogeneity liquid are expected to solve among the prior art finally.
In order to solve the technical problem, the utility model adopts the following technical scheme:
the utility model provides a portable liquid electrophoresis beam split detection device, including sample syringe needle, negative pressure extraction mechanism, the sample syringe needle is connected through turn shape miniflow way with the middle of the negative pressure extraction mechanism, turn shape miniflow way is close to sample syringe needle end branch respectively and is provided with optic fibre, power anodal, turn shape miniflow way is close to negative pressure extraction mechanism end branch respectively and is provided with power negative pole, photoelectric reception window, turn shape miniflow way turn department is provided with half anti-semi-permeable membrane, half anti-semi-permeable membrane outside is provided with photoelectric sensor, photoelectric sensor is connected with the external detection analytic system electricity.
Further, the shape of the turning-shaped microchannel is W-shaped.
Furthermore, three turning positions of the W-shaped turning micro-flow path are provided with three semi-reflecting and semi-permeable membranes, and three photoelectric sensors are arranged outside the three semi-reflecting and semi-permeable membranes.
Furthermore, a micro-filter is arranged between the sampling needle head and the turning-shaped micro-channel.
Further, the negative pressure pumping mechanism is a micro self-priming pump.
Compared with the prior art, the beneficial effects of the utility model are that: during detection by an electrophoresis spectroscopy method, a turning (W-shaped or similar) micro flow channel is designed, the electrophoresis method separates liquid (solution, turbid liquid, animal and plant body fluid and the like) led into the micro flow channel and dynamically tracks the change of a transmission spectrum of the liquid in the flow channel at the same time, so that specific components and properties in the liquid are detected, continuous dynamic detection and on-site real-time analysis are realized, initial characteristics, intermediate process characteristics and final characteristics recorded by a photoelectric sensor are analyzed, and the accurate expression of characteristic values of all particles (clusters) in a liquid sample is obtained; the device has small volume and can be widely used for rapid analysis and accurate detection of various liquids.
Drawings
Fig. 1 is a schematic structural view of a portable liquid electrophoresis spectroscopic detection device according to an embodiment of the present invention.
The reference numerals are illustrated below:
1 is a sampling needle head, 2 is a negative pressure extraction mechanism, 3 is a turning type micro-flow passage, 4 is an optical fiber, 5 is a power supply anode, 6 is a power supply cathode, 7 is a photoelectric receiving window, 8 is a semi-reflecting and semi-permeable membrane, 9 is a photoelectric sensor and 10 is a micro-filter.
Detailed Description
The present invention will be further explained with reference to the accompanying drawings.
Referring to fig. 1, the embodiment of the present invention is a portable liquid electrophoresis spectroscopic detection device, including a sampling needle 1, a negative pressure extraction mechanism 2, importantly, the sampling needle 1 is connected with the negative pressure extraction mechanism 2 through a turning type microchannel 3, the turning type microchannel 3 is provided with an optical fiber 4 and a power anode 5 respectively in a branch way near the sampling needle 1, the turning type microchannel 3 is provided with a power cathode 6 and a photoelectric receiving window 7 in a branch way near the negative pressure extraction mechanism 2, a turning point of the turning type microchannel 3 is provided with a semi-reflective and semi-transparent film 8, a photoelectric sensor 9 is arranged outside the semi-reflective and semi-transparent film 8, and the photoelectric sensor 9 is electrically connected with an external detection and analysis system; the turning-type micro-flow path 3 is designed, and through a semi-reflecting and semi-permeable membrane 8 and a photoelectric sensor 9 at the turning part of the turning-type micro-flow path 3, the electrophoresis method separates the liquid (solution, turbid liquid, animal and plant body fluid and the like) led into the micro-flow path and dynamically tracks the change of the transmission spectrum of the liquid in the flow path at the same time, so that specific components and properties in the liquid are detected, and continuous dynamic detection and on-site real-time analysis are realized.
In one embodiment, the turning-shaped microchannel 3 is W-shaped, so as to increase the flow path of the liquid and facilitate dynamic observation of spectral characteristics.
More specifically, three turning positions of the W-shaped turning micro flow path 3 are provided with three semi-reflective and semi-permeable membranes 8, and three photoelectric sensors 9 are provided outside the three semi-reflective and semi-permeable membranes 8.
Furthermore, a micro-filter 10 is disposed between the sampling needle 1 and the turning-type microchannel 3, and can be set according to the detection requirement, and the liquid to be detected is subjected to preliminary treatment in one step.
More specifically, the negative pressure pumping mechanism 2 is a micro self-priming pump, can be electrically connected with an external detection and analysis system and is controlled by the system, and can automatically pump a sample and perform a cleaning process.
The specific working process of the portable liquid electrophoresis light-splitting detection device is as follows:
1) the sampling needle 1 contacts with a sample, the negative pressure extraction mechanism 2 works, and a liquid sample enters and fills the turning-shaped microchannel 3 through the needle and the needle tube;
2) when the light source is turned on, light is guided into a light inlet at one end of the turning-shaped micro-channel 3 through the optical fiber 4, and is guided out from the tail end of the turning-shaped micro-channel 3 after being subjected to multiple light splitting and reflection through a light splitting/emitting/reflecting window at the turning point of the channel;
3) the photoelectric sensors 9 positioned at each position of the turning-type microchannel 3 collect and record the spectral characteristics of the initial liquid sample through the semi-reflecting and semi-permeable membrane 8;
4) electrifying the electrophoresis circuit, loading direct-current voltage on the electrophoresis electrode between the power supply anode 5 and the power supply cathode 6, forming an electric field in the liquid sample, starting to move various particles to the anode or the cathode in the electric field, wherein the moving speed depends on the characteristics of the charge quantity, the mass, the volume, the shape and the like of the particles; the light splitting characteristics of different positions of the liquid sample are changed continuously, and are recorded and stored by the photoelectric sensors of the light splitting/emitting/reflecting windows positioned at various positions;
5) after the whole electrophoresis process is finished, the particles in the liquid sample are redistributed, and the initial characteristics, the intermediate process characteristics and the final characteristics recorded by the photoelectric sensor 9 are analyzed to obtain the accurate expression of the characteristic value of each particle (cluster) in the liquid sample;
6) and finally, after the detection is finished, the negative pressure extraction mechanism 2 reversely acts to remove the liquid sample and extract purified water to clean the liquid path and prepare for the next detection.
In addition to the foregoing, it should be noted that reference throughout this specification to "one embodiment," "another embodiment," "an embodiment," or the like, means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment described generally throughout this application. The appearances of the same phrase in various places in the specification are not necessarily all referring to the same embodiment. Further, when a particular feature, structure, or characteristic is described in connection with any embodiment, it is submitted that it is within the scope of the invention to effect such feature, structure, or characteristic in connection with other embodiments.
Although the invention has been described herein with reference to a number of illustrative embodiments thereof, it should be understood that numerous other modifications and embodiments can be devised by those skilled in the art that will fall within the spirit and scope of the principles of this invention. More specifically, various variations and modifications are possible in the component parts and/or arrangements of the subject combination arrangement within the scope of the disclosure, the drawings and the appended claims. In addition to variations and modifications in the component parts and/or arrangements, other uses will also be apparent to those skilled in the art.
Claims (5)
1. The utility model provides a portable liquid electrophoresis beam split detection device, includes sample syringe needle, negative pressure extraction mechanism, its characterized in that: the utility model discloses a sampling needle, negative pressure extraction mechanism, including sampling needle, turning shape miniflow way, power negative pole, photoelectric receiving window, turning shape miniflow way, photoelectric sensing system, it is connected through turning shape miniflow way in the middle of sampling needle and the negative pressure extraction mechanism, turning shape miniflow way is close to sampling needle end branch respectively and is provided with optic fibre, power anodal, turning shape miniflow way is close to negative pressure extraction mechanism end branch respectively and is provided with power negative pole, photoelectric receiving window, turning shape miniflow way turning department is provided with the semi-reflection and semi-permeable membrane, the semi-reflection and semi-permeable membrane outside is provided with photoelectric sensor, photoelectric sensor is connected with the outside detection and analysis system electricity.
2. The portable liquid electrophoresis spectroscopy detection device of claim 1, wherein: the shape of the turning-folding micro flow path is W-shaped.
3. The portable liquid electrophoresis spectroscopy detection device of claim 2, wherein: three turning positions of the W-shaped turning micro-flow path are provided with three semi-reflecting and semi-permeable membranes, and three photoelectric sensors are arranged outside the three semi-reflecting and semi-permeable membranes.
4. The portable liquid electrophoresis spectroscopy detection apparatus of claim 1, wherein: a micro filter is also arranged between the sampling needle head and the turning-type micro-flow path.
5. The portable liquid electrophoresis spectroscopy detection apparatus of claim 1, wherein: the negative pressure pumping mechanism is a micro self-priming pump.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220904715.XU CN217180697U (en) | 2022-04-19 | 2022-04-19 | Portable liquid electrophoresis light splitting detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220904715.XU CN217180697U (en) | 2022-04-19 | 2022-04-19 | Portable liquid electrophoresis light splitting detection device |
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| Publication Number | Publication Date |
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| CN217180697U true CN217180697U (en) | 2022-08-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202220904715.XU Active CN217180697U (en) | 2022-04-19 | 2022-04-19 | Portable liquid electrophoresis light splitting detection device |
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| CN (1) | CN217180697U (en) |
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- 2022-04-19 CN CN202220904715.XU patent/CN217180697U/en active Active
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