CN217180593U - Nucleated red blood cell analysis device - Google Patents
Nucleated red blood cell analysis device Download PDFInfo
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- CN217180593U CN217180593U CN202220079070.0U CN202220079070U CN217180593U CN 217180593 U CN217180593 U CN 217180593U CN 202220079070 U CN202220079070 U CN 202220079070U CN 217180593 U CN217180593 U CN 217180593U
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Abstract
The utility model discloses a nucleated erythrocyte analytical equipment belongs to biomedical technical field. Particularly, the utility model provides a nucleated erythrocyte analytical equipment includes: the device comprises a light source module, a fluid module, a detector module and a data processing module; wherein the light source module comprises two light sources; the detector module comprises three detectors for detecting light sources in different directions to obtain corresponding data; the data processing module is used for processing the obtained optical signal. The utility model provides a detection device is used for detecting nucleated erythrocyte, need not increase fluorescent dye, belongs to the analytical equipment of no mark discernment nucleated erythrocyte. The utility model provides a nucleated erythrocyte analytical equipment can overcome traditional approach antibody coupling specificity poor, leads to detecting false positive height, and nucleated erythrocyte and leucocyte distinguish unobvious defect.
Description
Technical Field
The utility model belongs to the technical field of biomedicine. Particularly, the utility model relates to a nucleated erythrocyte analytical equipment.
Background
Erythrocytes are oxygen-carrying cells of the human body and are one of the most important components in blood. Usually, erythrocytes in peripheral blood do not contain nuclei, but in the case of human diseases such as hyperplastic anemia and leukemia, nucleated erythrocytes are present in peripheral blood. In addition, erythrocytes in the blood of the fetus are nuclear, and very few of these nucleated erythrocytes break the placental barrier into the maternal peripheral blood. The detection of the nucleated red blood cells is helpful for judging and screening the occurrence of diseases, particularly the nucleated red blood cells in the peripheral blood of the pregnant women, possibly the fetal nucleated red blood cells, and the sequencing of the nucleated red blood cells is beneficial to finding out the potential gene defects of the fetus, so that noninvasive prenatal diagnosis is realized.
There are presently some products, devices and methods disclosed for the analysis of nucleated red blood cells. Typical products such as the five-class hematology analyzer products of the Hesimecon and Mirey medical treatments, such as CN200610073296, use reagents to dissolve the cytoplasm of the nucleated erythrocyte apparatus, so that only the nucleus of the nucleated erythrocyte remains, and distinguish the nucleated erythrocyte from the white blood cell by means of optical scattering fluorescence and the like, thereby realizing the detection of the nucleated erythrocyte. CN201880098383 discloses a method for nucleated red blood cell counting by scattered light and fluorescence. In distinction to the five-classification optical detection method, beckmann coulter discloses a method for detecting nucleated red blood through a non-focusing hole in patent CN02814528, and the method realizes signal differentiation of nucleated red blood cells and white blood cells by the coulter electrical impedance principle, thereby realizing the detection of the nucleated red blood cells. In patent CN201810578067, detection and acquisition of fetal nucleated red blood cells are realized in a microfluidic chip by an antibody coupling method. Patent CN202010549815 discloses a method for obtaining nucleated red blood cells by means of antibodies and protein silk membranes.
The flow cytometer can also be used for analyzing the nucleated red blood cells, the nucleated red blood cells are marked by a fluorescent antibody coupling method, then the scattered light and the fluorescence intensity of the nucleated red blood cells are detected by the flow cytometer, the signals of the nucleated red blood cells and the white blood cells are effectively distinguished, and the nucleated red blood cells are analyzed.
The above device or technical method is based on the theory of judgment that when the content of nucleated red blood cells is low, the antibody for labeling nucleated red blood cells has a high probability of false positive under the statistical property of a huge amount of leukocytes, and the size distribution of the leukocytes is dispersed in a larger scale range and covers the scale range of the nucleated red blood cells, when the content of nucleated red blood cells in maternal peripheral blood is low, particularly when the fetal nucleated red blood cells in maternal peripheral blood only have the content of the leukocytes of several parts per million, the above method can hardly work.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide an analytical equipment of no mark discernment nucleated erythrocyte to it is poor to overcome traditional approach antibody coupling specificity, leads to detecting false positive height, and nucleated erythrocyte and leucocyte distinguish unobvious defect.
In order to achieve the purpose, the invention provides the following technical scheme:
a nucleated red blood cell analysis device, comprising:
a light source module, the light source module comprising:
a first light source emitting light having a first wavelength;
a second light source emitting light having a second wavelength, and
an optical element comprising beam combining and focusing optics of light;
a fluidic module, the fluidic module comprising:
a light-transmitting fluid channel for single sample particle flow sample introduction of a sample;
a focusing light spot for realizing single particle once-through light source;
a detector module, the detector module comprising:
a first detector to receive a forward scattered light signal of light having a first wavelength;
a second detector to receive a side scatter signal of light having a first wavelength;
a third detector to receive a side scatter light signal having light of a second wavelength;
and the data analysis processing module comprises a central processor and is used for realizing the processing of the detection signal.
Preferably, the first light source is a laser with the emission wavelength of 620-650 nm;
the second light source is a laser with the emission wavelength of 350-550 nm.
Preferably, the first light source is a laser emitting light with a wavelength of 638nm ± 10 nm;
the second light source is a laser with the emission wavelength of 405 +/-5 nm.
Preferably, the fluid channel is a rectangular quartz orifice with dimensions of 100-.
Preferably, the detector module further comprises a field stop;
according to the light running path, the field diaphragm is arranged in front of the first detector and used for limiting the range of the light received by the first detector.
Preferably, the detector module further comprises a narrow-band filter;
according to the light running path, the narrow-band filter is arranged in front of the first detector and used for limiting the wavelength of the light received by the first detector;
when the first light source emits light with a wavelength of 638nm + -10 nm, the narrowband filter is a 638 + -10 nm narrowband filter of OD 4.
Preferably, the detector module further comprises an optical lens group;
according to the light running path, the optical lens group is arranged in front of the first detector and used for imaging the focus of the light source within +/-3 mm near the photosensitive surface of the first detector.
Preferably, the detector module further comprises an aperture diaphragm;
according to the light running path, the aperture diaphragm is arranged in front of the first detector and used for controlling the numerical aperture value not larger than the aperture value of the focal length optical system of the light source module.
Preferably, the detector module comprises: an aperture diaphragm, an optical lens group, a narrow-band light filter and a field diaphragm;
according to the light running path, the aperture diaphragm, the optical lens group, the narrow-band filter and the field diaphragm are sequentially arranged in front of the first detector.
Preferably, the detector module further comprises:
according to the light path, an optical lens group, a dichroic mirror and a narrow-band light filter are arranged in front of the second detector and the third detector;
the optical lens group, the dichroic mirror and the narrow-band filter respectively guide light with different wavelengths into the second detector and the third detector according to the wavelength.
Compared with the prior art, the utility model discloses following profitable effect has:
the utility model provides a detection device is used for detecting nucleated erythrocyte, need not increase fluorochrome, belongs to the analytical equipment of no mark discernment nucleated erythrocyte. The utility model provides a nucleated erythrocyte analytical equipment can overcome traditional approach antibody coupling specificity poor, leads to detecting false positive height, and nucleated erythrocyte and leucocyte distinguish unobvious defect.
Drawings
Fig. 1 is a schematic view of the nucleated red blood cell analyzer according to the present invention.
Fig. 2 is a two-dimensional graph of lateral absorption coefficient versus cell number for analyzing nucleated red blood cells according to the present invention.
Fig. 3 is a two-dimensional graph of lateral absorption coefficient versus forward absorption light for analyzing nucleated red blood cells provided by the present invention.
In the figure, 1, a light source module; 2. a fluid module; 3. a detector module; 4. a data analysis processing module; 12. an optical element; 111. a first light source; 112. a second light source; 311. a first detector; 312. a field stop; 313. a narrow band filter; 314. an optical lens group; 315. an aperture diaphragm; 321. a second detector; 322. a third detector; 323. a dichroic mirror.
Detailed Description
In order to clearly understand the technical features, objects and advantages of the present invention, the following detailed description is given to the technical solution of the present invention, but the technical solution of the present invention is not limited to the limit of the implementable range of the present invention.
Throughout the specification, unless otherwise specifically noted, terms used herein should be understood as having meanings as commonly used in the art. Accordingly, unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. If there is a conflict, the present specification will control.
It should be noted that, in the present application, the terms "comprises", "comprising" or any other variation is intended to cover a non-exclusive inclusion, so that a method or apparatus including a series of elements includes not only the explicitly recited elements but also other elements not explicitly listed or inherent to the method or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other related elements in a method or apparatus that comprises the element.
It should be noted that the terms "first \ second \ third" referred to in the embodiments of the present application merely distinguish similar objects, and do not represent a specific ordering for the objects, and it should be understood that "first \ second \ third" may exchange a specific order or sequence when allowed. It should be understood that "first \ second \ third" distinct objects may be interchanged under appropriate circumstances such that the embodiments of the application described herein may be implemented in an order other than those illustrated or described herein.
It should be noted that the terms "a" and "an" in the present invention are used merely for convenience of description and are not to be construed as limiting the number.
As shown in fig. 1, the present invention provides a nucleated red blood cell analysis device. The nucleated red blood cell analysis device can be used for analyzing biological samples, such as blood, urine and the like. The nucleated red blood cell analysis device includes:
a light source module 1, said light source module 1 comprising:
a first light source 111 emitting light having a first wavelength;
a second light source 112 emitting light having a second wavelength, and
an optical element 12, said optical element 12 comprising beam combining and focusing optics of light;
a fluidic module 2, said fluidic module 2 comprising:
a light-transmitting fluid channel for single sample particle flow sample introduction of a sample;
a focusing light spot for realizing single particle once-through light source;
a detector module 3, the detector module 3 comprising:
a first detector 311 for receiving a forward scattered light signal of light having a first wavelength;
a second detector 321 to receive side scattered light signals of light having a first wavelength;
a third detector 322 to receive side scatter signals having light of a second wavelength;
a data analysis processing module 4, which includes a central processor for processing the detection signal.
The wavelengths of the light emitted by the two sets of light sources in the light source module 1, i.e. the first light source 111 and the second light source 112, fall in the high absorption region and the ground absorption region of hemoglobin, respectively. There is no requirement as to which light source emits light in the high absorption region and which light source emits light in the absorption region. For example:
in some specific embodiments:
the first light source 111 may be a laser emitting light with a wavelength of 620-650 nm;
the second light source 112 may be a laser emitting light with a wavelength of 350-550 nm; further:
the first light source 111 may be a laser emitting light with a wavelength of 638nm ± 10 nm;
the second light source 112 may be a laser emitting light having a wavelength of 405 ± 5 nm.
In other embodiments, the following may also be:
the first light source 111 may be a laser emitting light with a wavelength of 350-550 nm;
the second light source 112 may be a laser emitting at wavelengths of 620 and 650 nm: further, in the present invention,
the first light source 111 may be a laser emitting light having a wavelength of 405 ± 5 nm;
the second light source 112 is a laser emitting light with a wavelength of 638nm + -10 nm.
As an alternative embodiment, the fluid channel is a rectangular quartz orifice with dimensions of 100-.
As an alternative embodiment, the detector module 3 further includes a field stop 312;
the field stop 312 is disposed in front of the first detector 311 according to the traveling path of the light, so as to limit the range of the light received by the first detector 311.
As an alternative embodiment, the detector module 3 further includes a narrow band filter 313;
according to the light path, the narrow band filter 313 is arranged in front of the first detector 311 to limit the wavelength of the light received by the first detector 311;
when the first light source 111 emits light having a wavelength of 638nm ± 10nm, the narrowband filter 313 is the 638 ± 10nm narrowband filter 313 of OD 4.
As an alternative embodiment, the detector module 3 further includes an optical lens group 314;
the optical lens group 314 is disposed in front of the first detector 311 according to the light path, so as to image the focus of the light source within ± 3mm near the photosensitive surface of the first detector 311.
As an alternative embodiment, the detector module 3 further includes an aperture stop 315;
according to the light path, the aperture stop 315 is disposed in front of the first detector 311 to control the numerical aperture not greater than the aperture of the focal length optical system of the light source module 1.
As an alternative embodiment, the detector module 3 comprises: an aperture stop 315, an optical lens group 314, a narrow-band filter 313, a field stop 312;
according to the light path, the aperture diaphragm 315, the optical lens group 314, the narrow-band filter 313 and the field diaphragm 312 are sequentially arranged in front of the first detector 311.
As an alternative embodiment, the detector module 3 further comprises:
an optical lens group 314, a dichroic mirror 323 and a narrow-band filter 313 which are arranged in front of the second detector 321 and the third detector 322 according to the light path; light of different wavelengths is guided into the second detector 321 and the third detector 322, respectively, according to the wavelength.
As an alternative embodiment, the data analysis processing module 4 includes a set of board cards with functions of a data acquisition card, and after the data acquired by the board cards is transmitted to the computer, the computer realizes the analysis processing of the data.
A method for analyzing a sample by using the nucleated red blood cell analyzer provided by the present invention, comprising:
step S1: the sample containing the blood cells such as the erythroblast leukocyte is detected in the device described in the utility model, and after each cell passes through the light source, the light signal received by the first detector 311 is recorded as a, the light signal intensity generated by the second detector 321 is recorded as b, and the light signal generated by the third detector 322 is recorded as c;
step S2: setting a parameter k as b/c; recording the data generated by each cell through a light source as a, b, c, k;
step S3: taking the data a, b, c and k of all cells, taking a larger than a certain value as a parameter, removing cell debris data, and then taking k as an abscissa axis to perform histogram statistics, as shown in fig. 2. Classifying cells in the sample into nucleated red blood cells and white blood cells; preferably, k employs a logarithmic coordinate system.
Step S4: the data a, b, c and k of all the cells are divided by taking a larger than a certain value as a parameter, and then a two-dimensional point diagram of all the cells is drawn by taking a and k as coordinate axes, as shown in fig. 3. Classifying cells in the sample into nucleated red blood cells and white blood cells; preferably, k is in a logarithmic coordinate system.
The utility model provides a detection device is used for detecting nucleated erythrocyte, need not increase fluorescent dye, belongs to the analytical equipment of no mark discernment nucleated erythrocyte. The utility model provides a nucleated erythrocyte analytical equipment can overcome traditional approach antibody coupling specificity poor, leads to detecting false positive height, and nucleated erythrocyte and leucocyte distinguish unobvious defect.
The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited thereto. Various features of the invention may be substituted, modified and combined without departing from the spirit and substance of the invention as claimed, and such simple variations and combinations should also be considered as disclosed in the present invention, all falling within the scope of the invention.
Claims (4)
1. A nucleated red blood cell analysis apparatus, comprising:
a light source module, the light source module comprising:
a first light source emitting light having a first wavelength;
a second light source emitting light having a second wavelength, and
an optical element comprising beam combining and focusing optics of light;
a fluidic module, the fluidic module comprising:
a light-transmitting fluid channel for single sample particle flow sample introduction of a sample;
a focusing light spot for realizing single particle once-through light source;
a detector module, the detector module comprising:
a first detector to receive a forward scattered light signal of light having a first wavelength;
a second detector to receive a side scatter signal of light having a first wavelength;
a third detector to receive a side scatter light signal having light of a second wavelength;
wherein the detector module further comprises: an aperture diaphragm, an optical lens group, a narrow-band light filter and a field diaphragm;
according to the light running path, the aperture diaphragm, the optical lens group, the narrow-band filter and the field diaphragm are sequentially arranged in front of the first detector;
the aperture diaphragm is used for controlling the numerical aperture value not to be larger than the aperture value of a focal length optical system of the light source module;
the optical lens group is used for imaging the focus of the light source within +/-3 mm near the photosensitive surface of the first detector;
the narrow-band filter is used for limiting the wavelength of light received by the first detector;
when the first light source emits light with the wavelength of 638nm +/-10 nm, the narrow band filter is a 638 +/-10 nm narrow band filter of OD 4;
the field diaphragm is used for limiting the range of light received by the first detector;
wherein the detector module further comprises:
according to the light path, an optical lens group, a dichroic mirror and a narrow-band light filter are arranged in front of the second detector and the third detector;
the optical lens group, the dichroic mirror and the narrow-band filter respectively guide light with different wavelengths into the second detector and the third detector according to the wavelength,
and the data analysis processing module comprises a central processor and is used for processing the detection signal.
2. The nucleated red blood cell analysis device according to claim 1, wherein the first light source is a laser emitting at a wavelength of 620 and 650 nm;
the second light source is a laser with the emission wavelength of 350-550 nm.
3. A nucleated red blood cell analysis device according to claim 2, wherein said first light source is a laser emitting light having a wavelength of 638nm ± 10 nm;
the second light source is a laser with the emission wavelength of 405 +/-5 nm.
4. The nucleated red blood cell analysis device according to claim 1, wherein the fluid channel is a rectangular quartz orifice having dimensions of 100 and 400 μm.
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| CN202220079070.0U CN217180593U (en) | 2022-01-10 | 2022-01-10 | Nucleated red blood cell analysis device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114441480A (en) * | 2022-01-10 | 2022-05-06 | 中国科学院苏州生物医学工程技术研究所 | Nucleated red blood cell analysis device and analysis method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114441480A (en) * | 2022-01-10 | 2022-05-06 | 中国科学院苏州生物医学工程技术研究所 | Nucleated red blood cell analysis device and analysis method |
| CN114441480B (en) * | 2022-01-10 | 2024-01-26 | 中国科学院苏州生物医学工程技术研究所 | Nuclear erythrocyte analysis device and analysis method |
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