CN217127427U - Reaction device for culturing anaerobic microorganisms - Google Patents

Reaction device for culturing anaerobic microorganisms Download PDF

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Publication number
CN217127427U
CN217127427U CN202220541405.6U CN202220541405U CN217127427U CN 217127427 U CN217127427 U CN 217127427U CN 202220541405 U CN202220541405 U CN 202220541405U CN 217127427 U CN217127427 U CN 217127427U
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tank body
anaerobic microorganisms
reaction
reactor
tank
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黄禄
梁嘉瑜
许燕滨
陈可佟
叶泽军
孙烨
李宇馨
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Guangdong University of Technology
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Guangdong University of Technology
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Abstract

The embodiment of the utility model discloses cultivate reaction unit of anaerobism type microorganism, it includes: the reaction tank body comprises a tank body and a tank cover hermetically matched with the tank body and is used for placing and culturing anaerobic microorganisms; the feeding unit is hermetically arranged on the tank cover and is used for quantitatively conveying nutrient substances corresponding to the anaerobic microorganisms into the reaction tank body; the nitrogen stripping unit is hermetically arranged on the tank cover and used for blowing nitrogen into the reaction tank body so as to blow oxygen in the reaction tank body out of the tank; the exhaust unit is hermetically arranged on the tank cover and is used for balancing the internal and external air pressures of the reaction tank body during oxygen stripping; and the stirrer is hermetically arranged on the tank cover and used for stirring nutrient substances and anaerobic microorganisms in the reaction tank body so as to ensure that the nutrient substances are fully contacted with the anaerobic microorganisms. The reaction device of the utility model can form strict anaerobic environment, and has simple structure and low cost.

Description

Reaction device for culturing anaerobic microorganisms
Technical Field
The utility model relates to a microbial cultivation device field especially relates to a cultivate reaction unit of anaerobic type microorganism.
Background
With the development of diversification of modern society, the environmental pollution caused by the diversification is complicated, and the most prominent is the increase of nitrogen and phosphorus elements in sewage. In the field of sewage treatment, anaerobic microorganisms are an indispensable key role in completing denitrification and dephosphorization work. Among them, the anammox process is a new biological denitrification technology, and has attracted much attention in the field of biological denitrification of wastewater. At present, most of researches based on anaerobic ammonium oxidation bacteria are carried out by taking activated sludge containing anaerobic ammonium oxidation bacteria to construct a small biochemical tank, but the anaerobic ammonium oxidation bacteria grow very slowly and have long secondary generation time, the biological concentration of the anaerobic ammonium oxidation bacteria is difficult to improve in practice, the time required for constructing the biochemical tank is long, the material cost is expensive, and a strict anaerobic environment is difficult to realize. How to construct an anaerobic microbial reaction device with strict anaerobic environment and low cost becomes a problem to be solved urgently.
SUMMERY OF THE UTILITY MODEL
In view of the above, it is necessary to provide a reactor for culturing anaerobic microorganisms to solve the following problems in the prior art: in practice, the biological concentration of anaerobic ammonia oxidizing bacteria is difficult to increase in a biochemical pool, the time required for constructing the biochemical pool is long, the cost is high, and a strict anaerobic environment is difficult to realize.
The technical scheme of the utility model as follows:
a reaction device for culturing anaerobic microorganisms, comprising: the reaction tank body comprises a tank body and a tank cover hermetically matched with the tank body and is used for placing and culturing anaerobic microorganisms; the feeding unit is hermetically arranged on the tank cover and is used for quantitatively conveying nutrient substances corresponding to the anaerobic microorganisms into the reaction tank body; the nitrogen stripping unit is hermetically arranged on the tank cover and used for blowing nitrogen into the reaction tank body so as to blow oxygen in the reaction tank body out of the tank; the exhaust unit is hermetically arranged on the tank cover and used for balancing the internal and external air pressures of the reaction tank body during oxygen stripping; and the stirrer is hermetically arranged on the tank cover and used for stirring the nutrient substances in the reaction tank body and the anaerobic microorganisms so that the nutrient substances and the anaerobic microorganisms are fully contacted.
Optionally, the tank body comprises a tank body and a tank bottom for placing and culturing anaerobic microorganisms, and the tank bottom and the tank body are detachably combined into a whole.
Alternatively, the can bottom and the can body are detachably integrated by a spiral structure.
Optionally, a silica gel sealing ring for preventing liquid leakage is arranged on the spiral structure.
Optionally, the reaction tank body is of a transparent hollow cylinder structure, and the manufacturing material is an edible polypropylene material.
Optionally, the feeding unit includes a feeding pipeline passing through the tank cover to enter the reaction tank body, a metering pump for metering the feeding amount is arranged on the feeding pipeline, the metering pump is arranged outside the reaction tank body, and the feeding pipeline is made of a PVC hose.
Optionally, a check valve for preventing backflow is arranged on the feeding pipeline, and the check valve is arranged between the metering pump and the tank cover.
Optionally, scale marks for observing water level and various representation states of bacterial growth are arranged on the outer surface of the reaction tank body, and the scale marks are parallel to the central axis of the reaction tank body.
Optionally, the nitrogen stripping unit comprises a nitrogen input pipe penetrating through the tank cover and entering the reaction tank body, and the nitrogen input pipe is made of a PVC hose.
Optionally, the exhaust unit comprises an exhaust pipe penetrating through the tank cover into the reaction tank body, and the exhaust pipe is made of a PVC hose.
Adopt the embodiment of the utility model provides a, following beneficial effect has:
the utility model discloses a cultivate reaction unit of anaerobism type microorganism, including the retort body that is used for placing and cultivateing anaerobism type microorganism, be used for the ration to this internal nutrient substance's that carries anaerobism type microorganism to the retort feeding unit that corresponds, a nitrogen gas blows off the unit with blowing off this internal oxygen with blowing off the outer nitrogen gas of retort, the exhaust unit of the inside and outside atmospheric pressure of balanced retort body and be used for stirring this internal nutrient substance of retort and the agitator of anaerobism type microorganism when being used for blowing off oxygen, can form the strict anaerobic environment who cultivates anaerobism type microorganism, and simple structure and low cost.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the description below are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
Wherein:
FIG. 1 is a schematic view showing the overall structure of a reactor for culturing anaerobic microorganisms according to an embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
Referring to fig. 1, as can be seen from fig. 1, a reactor 100 for culturing anaerobic microorganisms according to the present embodiment includes:
the reaction tank body comprises a tank body 90 and a tank cover 70 which is in sealing fit with the tank body 90 and is used for placing and culturing anaerobic microorganisms. In the present embodiment, optionally, the tank body 90 and the tank cover 70 are fixed in a spiral sealing manner, the reaction tank body is of a transparent hollow cylindrical structure, and the material for making the reaction tank body is an edible polypropylene material. The utility model aims at providing a strict anaerobic environment, letting oxygen type microorganism survive in a small-size reaction unit, for this reason the utility model discloses regard as the retort body with stable nontoxic food level polypropylene (PP), be favorable to oxygen type microorganism to survive and breed in a small-size reaction unit.
And the feeding unit is hermetically arranged on the tank cover 70 and is used for quantitatively conveying nutrient substances corresponding to the anaerobic microorganisms into the reaction tank body. In the present embodiment, optionally, the feeding unit includes a feeding pipe 10 penetrating through the tank cover 70 into the reaction tank body, a metering pump 11 for metering the feeding amount is disposed on the feeding pipe 10, and the metering pump 11 is disposed outside the reaction tank body.
Wherein, the setting is used for measuring the beneficial effect of the measuring pump 11 of feeding quantity to lie in: for a small reaction device, it is unrealistic to provide nutrient substances for anaerobic microorganisms in a continuous water inlet and water outlet mode, and the microorganisms are damaged by a water changing mode, most anaerobic microorganisms are sensitive to environmental change, and frequent water changing can cause unstable growth environment, so that the anaerobic microorganisms are always in an environment-adaptive growth stage and cannot grow and proliferate well. The utility model discloses a setting is used for measuring the measuring pump 11 of feeding quantity, need not provide nutrient substance for anaerobic type microorganism with the mode of constantly intaking and going out water, can use measuring pump 11 to come for it provides nutrient substance according to anaerobic type microorganism's growth law, is favorable to anaerobic type microorganism to live and breed in reaction unit.
In the present embodiment, optionally, a check valve 12 for preventing backflow is disposed on the feeding pipe 10, and the check valve 12 is disposed between the metering pump 11 and the tank cover 70. In addition, optionally, the material of the feeding pipe 10 is PVC (Polyvinyl Chloride) hose, which is relatively flexible and easy to place, and has a relatively smooth inner surface, and is suitable for being used as the feeding pipe 10.
And a nitrogen stripping unit hermetically provided on the tank cover 70 for blowing nitrogen into the reaction tank body to blow oxygen in the reaction tank body out of the tank.
And an exhaust unit hermetically provided on the can cover 70 for balancing internal and external air pressures of the reaction can body when oxygen is blown off.
And a stirrer 40 hermetically provided on the can cover 70 for stirring the nutrient substances in the reaction can body and the anaerobic microorganisms to make the nutrient substances and the anaerobic microorganisms sufficiently contact.
In this embodiment, the tank body 90 optionally includes a tank body 60 and a tank bottom 50 for placing and culturing anaerobic microorganisms, and the tank bottom 50 and the tank body 60 are detachably integrated. Wherein, tank bottom 50 can be relatively the body 60 separates, is favorable to the staff to get the fungus sample and carries out the experiment analysis, need not to open the sample of cover 70.
In this embodiment, optionally, the can bottom 50 and the can body 60 are detachably integrated by a spiral structure 51.
In this embodiment, a silicone sealing ring (not shown) for preventing liquid leakage is optionally disposed on the spiral structure 51.
In this embodiment, optionally, scale marks 80 for observing the water level and various characterization states of bacteria growth are provided on the outer surface of the reaction tank body, and the scale marks 80 are parallel to the central axis of the reaction tank body.
In this embodiment, optionally, the nitrogen stripping unit includes a nitrogen input pipe 20 penetrating through the tank cover 70 into the reaction tank body, and the nitrogen input pipe 20 is made of a PVC hose.
In this embodiment, optionally, the exhaust unit includes an exhaust pipe 30 penetrating through the tank cover 70 into the reaction tank body, and the exhaust pipe 30 is made of a PVC hose.
The utility model discloses a cultivate reaction unit 100 of anaerobism type microorganism, including the retort body that is used for placing and cultivateing anaerobism type microorganism, be used for the ration to this internal nutrient substance's that carries anaerobism type microorganism to the retort feeding unit that corresponds, be used for blowing nitrogen gas in order to blow out this internal oxygen of retort outside the jar the nitrogen gas blow-off unit, the exhaust unit of the inside and outside atmospheric pressure of balanced retort body and be used for stirring this internal nutrient substance of retort and the agitator 40 of anaerobism type microorganism when being used for blowing off oxygen, can form the strict anaerobic environment of cultivateing anaerobism type microorganism, and simple structure and low cost.
The above disclosure is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the scope of the invention, which is defined by the appended claims.

Claims (10)

1. A reactor device for culturing anaerobic microorganisms, comprising:
the reaction tank body comprises a tank body and a tank cover hermetically matched with the tank body and is used for placing and culturing anaerobic microorganisms;
the feeding unit is hermetically arranged on the tank cover and is used for quantitatively conveying nutrient substances corresponding to the anaerobic microorganisms into the reaction tank body;
the nitrogen stripping unit is hermetically arranged on the tank cover and used for blowing nitrogen into the reaction tank body so as to blow oxygen in the reaction tank body out of the tank;
the exhaust unit is hermetically arranged on the tank cover and used for balancing the internal and external air pressures of the reaction tank body during oxygen stripping;
and the stirrer is hermetically arranged on the tank cover and used for stirring the nutrient substances in the reaction tank body and the anaerobic microorganisms so that the nutrient substances and the anaerobic microorganisms are fully contacted.
2. The reactor for cultivating anaerobic microorganisms according to claim 1, wherein said tank body comprises a tank body and a tank bottom for housing and cultivating anaerobic microorganisms, said tank bottom and said tank body being detachably integrated.
3. The reactor for cultivating anaerobic microorganisms according to claim 2, wherein said tank bottom and said tank body are detachably integrated by a spiral structure.
4. The reactor for culturing anaerobic microorganisms according to claim 3, wherein the spiral structure is provided with a silicone packing for preventing liquid leakage.
5. The reactor for culturing anaerobic microorganisms according to claim 1, wherein the reactor body is a transparent hollow cylinder structure and is made of food grade polypropylene.
6. The apparatus of claim 1, wherein the feeding unit comprises a feeding pipe passing through the tank cover and entering the reaction tank body, the feeding pipe is provided with a metering pump for metering the feeding amount, the metering pump is arranged outside the reaction tank body, and the feeding pipe is made of PVC hose.
7. The apparatus of claim 6, wherein a check valve for preventing backflow is provided on the feeding pipe, and the check valve is provided between the metering pump and the tank cover.
8. The reactor for culturing anaerobic microorganisms according to claim 1, wherein a scale mark for observing the water level is provided on the outer surface of the reactor body, and the scale mark is parallel to the central axis of the reactor body.
9. The reaction device for cultivating anaerobic microorganisms, according to claim 1, wherein the nitrogen stripping unit comprises a nitrogen input pipe passing through the tank cover into the reaction tank body, and the nitrogen input pipe is made of a PVC hose.
10. The reactor for cultivating anaerobic microorganisms according to claim 1, wherein the exhaust unit comprises an exhaust pipe passing through the tank cover into the reactor body, and the exhaust pipe is made of a PVC hose.
CN202220541405.6U 2022-03-11 2022-03-11 Reaction device for culturing anaerobic microorganisms Active CN217127427U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202220541405.6U CN217127427U (en) 2022-03-11 2022-03-11 Reaction device for culturing anaerobic microorganisms

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202220541405.6U CN217127427U (en) 2022-03-11 2022-03-11 Reaction device for culturing anaerobic microorganisms

Publications (1)

Publication Number Publication Date
CN217127427U true CN217127427U (en) 2022-08-05

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Application Number Title Priority Date Filing Date
CN202220541405.6U Active CN217127427U (en) 2022-03-11 2022-03-11 Reaction device for culturing anaerobic microorganisms

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CN (1) CN217127427U (en)

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