CN217111983U - Sample loading tube for detecting human mesenchymal stem cells - Google Patents
Sample loading tube for detecting human mesenchymal stem cells Download PDFInfo
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- CN217111983U CN217111983U CN202220452716.5U CN202220452716U CN217111983U CN 217111983 U CN217111983 U CN 217111983U CN 202220452716 U CN202220452716 U CN 202220452716U CN 217111983 U CN217111983 U CN 217111983U
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- connecting rod
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- mesenchymal stem
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Abstract
The utility model relates to the technical field of cell detection, in particular to a sample loading tube for detecting human mesenchymal stem cells, which is convenient for accurately guiding the flow of the mesenchymal stem cells into a flow cytometer by arranging the device; including flow cytometer, go up the appearance pipe, first flitch, the pull rod, a housing, the second flitch, first connecting rod, first baffle, first spring, the apparatus further comprises a rotating shaft, the second connecting rod, the third connecting rod, including a motor, an end cap, a controller, and a cover plate, second baffle, second spring and connecting device, it installs in flow cytometer to go up the appearance pipe through connecting device, it is provided with the material mouth to go up appearance pipe bottom intercommunication, first flitch slides in last appearance pipe, second connecting rod and third connecting rod rotatable coupling, the third connecting rod is connected with the motor output, the motor is installed at the casing front end, second baffle rotatable mounting is in casing and last appearance pipe intercommunication position, second spring one end is connected with the second baffle, the second spring other end is connected with the casing internal top.
Description
Technical Field
The utility model relates to a technical field that cell detected especially relates to a sample loading pipe for human mesenchymal stem cell detects.
Background
Flow cytometers are devices that automatically analyze and sort cells. It can quickly measure, store and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in liquid, and can select specified cell subsets according to the preselected parameter range. Most flow cytometers are zero resolution instruments that can only measure an index such as total nucleic acid, total protein, etc. of a cell, but cannot identify and measure the amount of nucleic acid or protein at a particular location. That is to say, its detail resolution ratio is zero, installs the sample loading pipe in the flow cytometer, and the sample loading pipe is introduced mesenchymal stem cell into the flow cytometer according to the demand and is detected.
Because the sample loading quantity required by the flow cytometer is small, the existing sample loading pipe is inconvenient to accurately control the sample loading flow.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem, the utility model provides a be convenient for accurate leading-in to the flow of flow cytometer internal mesenchymal stem cell of accuse is used for people's mesenchymal stem cell to detect and goes up appearance pipe.
The utility model discloses a sample loading tube for human mesenchymal stem cell detection, including flow cytometer, sample loading tube, first flitch, pull rod, casing, second flitch, first connecting rod, first baffle, first spring, pivot, second connecting rod, third connecting rod, motor, second baffle, second spring and connecting device, sample loading tube installs in flow cytometer through connecting device, sample loading tube bottom intercommunication is provided with the material mouth, first flitch slides in sample loading tube, first flitch top is connected with the pull rod bottom, pull rod and sample loading tube top sliding connection, the casing is installed in sample loading tube right-hand member lower part, casing and sample loading tube intercommunication, second flitch slides in the casing, second flitch right-hand member is connected with first connecting rod left end, first connecting rod and casing right-hand member sliding connection, casing top intercommunication is provided with the inlet port, first baffle can rotate to be installed in the inlet port bottom, one end of a first spring is connected with the air inlet, the other end of the first spring is connected with a first baffle, the first connecting rod is rotatably connected with a second connecting rod through a rotating shaft, the second connecting rod is rotatably connected with a third connecting rod, the third connecting rod is connected with the output end of a motor, the motor is installed at the front end of the shell, the second baffle is rotatably installed at the position where the shell is communicated with the sample loading pipe, one end of the second spring is connected with the second baffle, and the other end of the second spring is connected with the top end in the shell.
The utility model discloses a go up appearance pipe for human mesenchymal stem cell detects, connecting device include two sets of dops, spring and two sets of push rods, go up the appearance pipe left end and be provided with the spout, and two sets of dops slide in the spout, and two sets of dops pass through spring coupling, and the left side is provided with the draw-in groove in the flow cytometer, and two sets of dops are connected with the draw-in groove cooperation, and two sets of push rods are installed respectively in two sets of dop outer ends.
The utility model discloses a go up appearance pipe for human mesenchymal stem cell detects still includes the safety cover, and the safety cover is installed in last appearance pipe right-hand member lower part, and the safety cover is located the casing, the first connecting rod, pivot, second connecting rod, third connecting rod and the motor outside.
The utility model discloses a go up appearance pipe for human mesenchymal stem cell detects still includes the pull ring, and the pull ring is installed on the pull rod top.
The utility model discloses a go up appearance pipe for human mesenchymal stem cell detects still includes flexible pipe and telescopic link, and flexible pipe and telescopic link all are located the spring inboard, and the telescopic link slides in flexible pipe, and flexible pipe and telescopic link outer end are connected with two sets of dops respectively.
The utility model discloses a go up appearance pipe for human mesenchymal stem cell detects still includes two sets of handles, and two sets of handles are installed respectively in two sets of push rod outer ends.
The utility model discloses a go up appearance pipe for human mesenchymal stem cell detects, the first baffle outside is provided with sealed the pad.
The utility model discloses a go up appearance pipe for human mesenchymal stem cell detects, the coating has lubricating oil in the spout.
Compared with the prior art, the beneficial effects of the utility model are that: the material inlet is immersed into the mesenchymal stem cells by moving the sample loading tube, then the first material plate is pulled by the pull rod, the mesenchymal stem cells are guided into the sample loading tube through the material inlet, then the sample loading tube is installed in the flow cytometer through the connecting device, then the motor is turned on, the third connecting rod drives the second connecting rod to swing, the second connecting rod drives the first connecting rod to slide left and right at the right end of the shell through the rotating shaft, the first connecting rod drives the second material plate to slide left and right in the shell, when the second material plate slides rightwards, the air pushes the first baffle plate to be opened and enter the shell, when the second material plate slides leftwards, the air pushes the second baffle plate to be opened and led into the inner part of the sample feeding pipe, the second spring generates elastic deformation, the air in the sample feeding pipe leads the mesenchymal stem cells into the flow cytometer through the material port for detection, through setting up this equipment, be convenient for accurate leading-in flow to flow cytometer internal mesenchymal stem cells's of accuse flow.
Drawings
Fig. 1 is a schematic structural diagram of the present invention;
FIG. 2 is a schematic cross-sectional view of FIG. 1;
fig. 3 is a schematic top view of the present invention;
FIG. 4 is an enlarged schematic view of A in FIG. 2;
FIG. 5 is an enlarged schematic view of B of FIG. 3;
in the drawings, the reference numbers: 1. a flow cytometer; 2. a sample loading pipe; 3. a material port; 4. a chute; 5. clamping a head; 6. a spring; 7. a card slot; 8. a push rod; 9. a first material plate; 10. a pull rod; 11. a housing; 12. a second material plate; 13. a first link; 14. an air inlet; 15. a first baffle plate; 16. a first spring; 17. a rotating shaft; 18. a second link; 19. a third link; 20. a motor; 21. a protective cover; 22. a pull ring; 23. a telescopic pipe; 24. a telescopic rod; 25. a handle; 26. a second baffle; 27. a second spring.
Detailed Description
The following detailed description of the embodiments of the present invention is provided with reference to the accompanying drawings and examples. The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention.
As shown in fig. 1 to 5, the sample loading tube for human mesenchymal stem cell detection of the present invention comprises a flow cytometer 1, a sample loading tube 2, a first material plate 9, a pull rod 10, a housing 11, a second material plate 12, a first connecting rod 13, a first baffle 15, a first spring 16, a rotating shaft 17, a second connecting rod 18, a third connecting rod 19, a motor 20, a second baffle 26, a second spring 27 and a connecting device, wherein the sample loading tube 2 is installed in the flow cytometer 1 through the connecting device, a material port 3 is provided at the bottom end of the sample loading tube 2, the first material plate 9 slides in the sample loading tube 2, the top end of the first material plate 9 is connected with the bottom end of the pull rod 10, the pull rod 10 is connected with the top end of the sample loading tube 2 in a sliding manner, the housing 11 is installed at the lower portion of the right end of the sample loading tube 2, the housing 11 is connected with the sample loading tube 2, the second material plate 12 slides in the housing 11, the right end of the second material plate 12 is connected with the left end of the first connecting rod 13, the first connecting rod 13 is connected with the right end of the shell 11 in a sliding mode, the top end of the shell 11 is communicated with an air inlet hole 14, a first baffle plate 15 is rotatably installed at the bottom of the air inlet hole 14, one end of a first spring 16 is connected with the air inlet hole 14, the other end of the first spring 16 is connected with the first baffle plate 15, the first connecting rod 13 is rotatably connected with a second connecting rod 18 through a rotating shaft 17, the second connecting rod 18 is rotatably connected with a third connecting rod 19, the third connecting rod 19 is connected with the output end of a motor 20, the motor 20 is installed at the front end of the shell 11, a second baffle plate 26 is rotatably installed at the position, where the shell 11 is communicated with the sample feeding pipe 2, one end of a second spring 27 is connected with the second baffle plate 26, and the other end of the second spring 27 is connected with the inner top end of the shell 11; the material inlet 3 is immersed into the mesenchymal stem cells by moving the sample loading tube 2, then the first material plate 9 is pulled by the pull rod 10, the mesenchymal stem cells are led into the sample loading tube 2 through the material inlet 3, then the sample loading tube 2 is installed in the flow cytometer 1 through the connecting device, then the motor 20 is turned on, the third connecting rod 19 drives the second connecting rod 18 to swing, the second connecting rod 18 drives the first connecting rod 13 to slide left and right at the right end of the shell 11 through the rotating shaft 17, the first connecting rod 13 drives the second material plate 12 to slide left and right in the shell 11, when the second material plate 12 slides right, the air pushes the first baffle plate 15 to open and enter the shell 11, when the second material plate 12 slides left, the air pushes the second baffle plate 26 to open and lead into the sample loading tube 2, the second spring 27 generates elastic deformation, the air in the sample loading tube 2 leads the mesenchymal stem cells into the flow cytometer 1 through the material inlet 3 for detection, through setting up this equipment, be convenient for accurate leading-in flow to 1 interior mesenchymal stem cells of flow cytometer of accuse.
The utility model discloses a sample loading pipe for human mesenchymal stem cell detects, connecting device include two sets of dop 5, spring 6 and two sets of push rod 8, sample loading pipe 2 left end is provided with spout 4, and two sets of dop 5 slide in spout 4, and two sets of dop 5 pass through spring 6 to be connected, and flow cytometer 1 is interior left side and is provided with draw-in groove 7, and two sets of dop 5 are connected with draw-in groove 7 cooperation, and two sets of push rod 8 are installed respectively in two sets of dop 5 outer ends; insert two sets of dops 5 to draw-in groove 7 in, sample tube 2 is then installed inside flow cytometer 1, when sample tube 2 dismantles the sample as required, through pressing two sets of push rods 8, two sets of push rods 8 then drive two sets of dops 5 and inwards slide in spout 4, elastic deformation then takes place for push rod 8, later takes out two sets of dops 5 in draw-in groove 7 to dismantle sample tube 2, be convenient for install the dismantlement to sample tube 2.
The utility model discloses a sample loading pipe for human mesenchymal stem cell detection still includes safety cover 21, and safety cover 21 installs in sample loading pipe 2 right-hand member lower part, and safety cover 21 is located the casing 11, first connecting rod 13, pivot 17, second connecting rod 18, third connecting rod 19 and the motor 20 outside; the protective cover 21 is provided to protect the housing 11, the first link 13, the rotation shaft 17, the second link 18, the third link 19, and the motor 20.
The utility model discloses a sample loading tube for detecting human mesenchymal stem cells, which also comprises a pull ring 22, wherein the pull ring 22 is arranged at the top end of the pull rod 10; by providing the pull ring 22, the operation of the pull rod 10 is facilitated, and the pull rod 10 is prevented from entering the interior of the loading sample tube 2.
The utility model discloses a go up appearance pipe for human mesenchymal stem cell detects still includes flexible pipe 23 and telescopic link 24, and flexible pipe 23 and telescopic link 24 all are located the inside of spring 6, and telescopic link 24 slides in flexible pipe 23, and flexible pipe 23 and telescopic link 24 outer end are connected with two sets of dops 5 respectively; by arranging the telescopic pipe 23 and the telescopic rod 24, the spring 6 can be supported and guided, and the stability of the chuck 5 is improved.
The utility model discloses a sample loading tube for detecting human mesenchymal stem cells, which also comprises two groups of handles 25, wherein the two groups of handles 25 are respectively arranged at the outer ends of the two groups of push rods 8; by providing a handle 25, it is convenient to press the push rod 8.
The utility model discloses a sample loading tube for detecting human mesenchymal stem cells, a sealing gasket is arranged outside the first baffle 15; the sealability of the first shutter 15 to the intake holes 14 can be improved.
The utility model discloses a sample loading pipe for human mesenchymal stem cell detects, the coating has lubricating oil in the spout 4; can act as a lubrication for the cartridge 5.
The utility model discloses a sample loading pipe for human mesenchymal stem cell detects, it is at work, at first make material mouth 3 dip in mesenchymal stem cell through removing sample loading pipe 2, then stimulate first flitch 9 through pull rod 10, mesenchymal stem cell then leads to in sample loading pipe 2 through material mouth 3, then install sample loading pipe 2 in flow cytometer 1 through connecting device, later open motor 20, third connecting rod 19 then drives second connecting rod 18 and swings, second connecting rod 18 then drives first connecting rod 13 through pivot 17 and slides from side to side at the casing 11 right-hand member, first connecting rod 13 then drives second flitch 12 and slides from side to side in casing 11, when second flitch 12 slides from side to side, the air then promotes first baffle 15 and opens and enters into casing 11, when second flitch 12 slides from side to side, air promotes second baffle 26 and opens and leads to the inside sample loading pipe 2, second spring 27 then takes place elastic deformation, the air in the sample loading tube 2 guides the mesenchymal stem cells into the flow cytometer 1 through the material port 3 for detection.
The sample loading tube for detecting the human mesenchymal stem cells of the utility model adopts a common mechanical mode, such as an installation mode, a connection mode or a setting mode, and can be implemented as long as the beneficial effects are achieved; the utility model discloses a flow cytometer 1 and motor 20 that is used for sample loading pipe of human mesenchymal stem cell to detect are the purchase on the market, and this industry technical staff only need install and operate according to its subsidiary instructions.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.
Claims (8)
1. A sample loading tube for detecting human mesenchymal stem cells is characterized by comprising a flow cytometer (1), a sample loading tube (2), a connecting device and a sample loading device, wherein the sample loading tube (2) is installed in the flow cytometer (1) through the connecting device, the bottom end of the sample loading tube (2) is communicated with a material port (3), and the sample loading device is arranged in the sample loading tube (2);
the sample loading device comprises a first material plate (9), a pull rod (10), a shell (11), a second material plate (12), a first connecting rod (13), a first baffle (15), a first spring (16), a rotating shaft (17), a second connecting rod (18), a third connecting rod (19), a motor (20), a second baffle (26) and a second spring (27), wherein the first material plate (9) slides in an upper sample tube (2), the top end of the first material plate (9) is connected with the bottom end of the pull rod (10), the pull rod (10) is connected with the top end of the upper sample tube (2) in a sliding manner, the shell (11) is installed at the lower part of the right end of the upper sample tube (2), the shell (11) is communicated with the upper sample tube (2), the second material plate (12) slides in the shell (11), the right end of the second material plate (12) is connected with the left end of the first connecting rod (13), the first connecting rod (13) is connected with the right end of the shell (11) in a sliding manner, and the top end of the shell (11) is communicated with an air inlet hole (14), first baffle (15) rotatable mounting is in inlet port (14) bottom, first spring (16) one end is connected with inlet port (14), first spring (16) other end is connected with first baffle (15), first connecting rod (13) are through pivot (17) and second connecting rod (18) rotatable coupling, second connecting rod (18) and third connecting rod (19) rotatable coupling, third connecting rod (19) are connected with motor (20) output, motor (20) are installed at casing (11) front end, second baffle (26) rotatable mounting is in casing (11) and sample loading pipe (2) intercommunication position, second spring (27) one end is connected with second baffle (26), the top is connected in second spring (27) other end and casing (11).
2. The loading tube for detecting the human mesenchymal stem cells as claimed in claim 1, wherein the connecting device comprises two sets of chucks (5), a spring (6) and two sets of push rods (8), the left end of the loading tube (2) is provided with a sliding groove (4), the two sets of chucks (5) slide in the sliding groove (4), the two sets of chucks (5) are connected through the spring (6), the left side in the flow cytometer (1) is provided with a clamping groove (7), the two sets of chucks (5) are connected with the clamping groove (7) in a matching way, and the two sets of push rods (8) are respectively installed at the outer ends of the two sets of chucks (5).
3. The loading tube for detecting the human mesenchymal stem cells, according to claim 2, further comprising a protective cover (21), wherein the protective cover (21) is installed at the lower part of the right end of the loading tube (2), and the protective cover (21) is positioned at the outer side of the shell (11), the first connecting rod (13), the rotating shaft (17), the second connecting rod (18), the third connecting rod (19) and the motor (20).
4. The loading tube for human mesenchymal stem cell assay according to claim 3, further comprising a pull ring (22), wherein the pull ring (22) is mounted on the top end of the pull rod (10).
5. The sample loading tube for detecting the human mesenchymal stem cells according to claim 4, further comprising a telescopic tube (23) and a telescopic rod (24), wherein the telescopic tube (23) and the telescopic rod (24) are both located inside the spring (6), the telescopic rod (24) slides in the telescopic tube (23), and the outer ends of the telescopic tube (23) and the telescopic rod (24) are respectively connected with the two groups of chucks (5).
6. The loading tube for detecting the human mesenchymal stem cells according to claim 5, further comprising two groups of handles (25), wherein the two groups of handles (25) are respectively arranged at the outer ends of the two groups of push rods (8).
7. The loading tube for detecting human mesenchymal stem cells according to claim 6, wherein a sealing gasket is arranged outside the first baffle (15).
8. The loading tube for human mesenchymal stem cell assay according to claim 7, wherein the inside of the sliding groove (4) is coated with a lubricating oil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220452716.5U CN217111983U (en) | 2022-03-02 | 2022-03-02 | Sample loading tube for detecting human mesenchymal stem cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220452716.5U CN217111983U (en) | 2022-03-02 | 2022-03-02 | Sample loading tube for detecting human mesenchymal stem cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217111983U true CN217111983U (en) | 2022-08-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220452716.5U Active CN217111983U (en) | 2022-03-02 | 2022-03-02 | Sample loading tube for detecting human mesenchymal stem cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217111983U (en) |
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2022
- 2022-03-02 CN CN202220452716.5U patent/CN217111983U/en active Active
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