CN217093567U

CN217093567U - VEGF antigen ultrafiltration centrifugal tube

Info

Publication number: CN217093567U
Application number: CN202221022738.4U
Authority: CN
Inventors: 白锟
Original assignee: Langfang Tian Guang Biological Technology Co ltd
Current assignee: Langfang Tian Guang Biological Technology Co ltd
Priority date: 2022-04-29
Filing date: 2022-04-29
Publication date: 2022-08-02
Anticipated expiration: 2032-04-29

Abstract

The utility model discloses a VEGF antigen ultrafiltration centrifugal tube, which comprises a tube body, a tube cover, a molecular weight cut-off inner tube and a connecting component; the tube body is clamped with the molecular weight cutoff inner tube through a fixing ring; the pipe cover comprises a cover body and a reinforcing mechanism; the cover body is an annular cylinder body; the bottom end of the cover body is inserted with the top end of the inner cavity of the tube body; the reinforcing mechanism is fixedly arranged at the bottom end of the inner cavity of the cover body; the reinforcing mechanism is abutted against the top surface of the molecular weight cutoff inner pipe; the cover body is detachably connected with the pipe body through the connecting assembly. The utility model discloses can realize improving the stability of experiment, reduce the cross contamination who filters the stoste.

Description

VEGF antigen ultrafiltration centrifugal tube

Technical Field

The utility model relates to an ultrafiltration technical field especially relates to a VEGF antigen ultrafiltration centrifuging tube.

Background

An ultrafiltration centrifugal tube is a separation and purification tool commonly used in life sciences and molecular biology laboratories. The mode of separating protein is mainly based on the molecular weight of protein, the ultrafiltration is completed by the centrifugal force of a centrifugal machine, the small molecular protein passes through a filter membrane under the action of the centrifugal force, and the large molecular protein sample is intercepted in a collecting pipe. Wherein the VEGF (vascular endothelial growth factor) ultrafiltration centrifugal tube is specially developed for VEGF antigen purification. However, the tube body and the tube cap of the existing ultrafiltration centrifugal tube are separated, so that the tube body and the tube cap are quite easy to lose or confuse, cross contamination of a filtration stock solution is caused, and the experiment fails.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a VEGF antigen ultrafiltration centrifuging tube to solve the problem that above-mentioned prior art exists, can realize improving the stability of experiment, reduce the cross contamination who filters the stoste.

In order to achieve the above object, the utility model provides a following scheme:

a VEGF antigen ultrafiltration centrifugal tube comprises a tube body, a tube cover, a molecular weight cut-off inner tube and a connecting assembly; the tube body is clamped with the molecular weight cutoff inner tube through a fixing ring; the pipe cover comprises a cover body and a reinforcing mechanism; the cover body is an annular cylinder body; the bottom end of the cover body is inserted with the top end of the inner cavity of the tube body; the reinforcing mechanism is fixedly arranged at the bottom end of the inner cavity of the cover body; the reinforcing mechanism is abutted against the top surface of the molecular weight cutoff inner pipe; the cover body and the pipe body are detachably connected through the connecting component.

Preferably, the reinforcing mechanism comprises a fixing block; a screw hole is formed in the center of the fixed block; a stop plug is screwed in the screw hole; the side surface and the bottom surface of the fixed block are provided with a plurality of push holes and a plurality of press holes; the pushing holes and the pressing holes are arranged in a one-to-one correspondence manner and are communicated with each other; the inner cavity of the push hole and the inner cavity of the press hole are respectively provided with a material pressing component; the material pressing component is abutted against the top surface of the molecular weight cutoff inner pipe.

Preferably, the swaging assembly comprises a first wedge and a second wedge; inclined planes are respectively poured at one end of the first wedge column and one end of the second wedge column; the first wedge column is connected with the pressure hole in a sliding mode; the second wedge column is connected with the push hole in a sliding mode; the two inclined planes are matched and correspondingly arranged; a sealing layer is fixedly connected to the bottom surface of the pressure hole; the bottom surface of the first wedge column is abutted against the top surface of the sealing layer; the bottom surface of the sealing layer is abutted against the top surface of the molecular weight cut-off inner pipe; the other end of the second wedge column is detachably connected with the side face of the blocking plug.

Preferably, the molecular weight cut-off inner tube is a polyethylene conical tube; the top surface of the molecular weight cutoff inner pipe is fixedly connected with a plurality of butt straps; the top surface of the access board is arranged in a manner of abutting against the sealing layer.

Preferably, the connecting assembly comprises two connecting plates; two connecting plate one end respectively with body lateral surface with lid lateral surface fixed connection, two the other end of connecting plate passes through elasticity strip fixed connection.

Preferably, the inner side surface of the fixing ring is matched with the outer side surface of the molecular weight cut-off inner tube.

Preferably, a note is stuck to the side surface of the cover body.

Preferably, the second wedge column is made of stainless steel; the sealing layer is a high-elasticity rubber layer.

The utility model discloses has following technological effect:

the utility model discloses a lid and body disjunctor design have avoided losing or the confusion of lid, have just also further avoided cross contamination's possibility. Meanwhile, the molecular weight cutoff inner tube is pressurized through the reinforcing mechanism, the phenomenon that the molecular weight cutoff inner tube is unstably placed or even broken due to slight vibration under the high-speed rotation of the centrifugal machine is avoided, and the safety of an experiment is improved. And the reinforcing mechanism does not need to input power additionally, but only utilizes the centrifugal force of the centrifugal machine to realize the pressurization of the molecular weight cutoff inner pipe, thereby optimizing the product structure and reducing the production cost.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.

Fig. 1 is a top view of the present invention.

Fig. 2 is a schematic sectional structure view of the section a-a in fig. 1.

Fig. 3 is an enlarged schematic view of B in fig. 2.

Fig. 4 is a side view of the reinforcing mechanism.

Wherein, 1, a tube body; 2. a tube cover; 21. a cover body; 22. a reinforcement mechanism; 221. a fixed block; 222. blocking the plug; 223. pushing the hole; 224. pressing a hole; 225. a first wedge column; 226. a second wedge column; 227. a sealing layer; 3. a molecular weight cut-off inner tube; 4. a fixing ring; 5. a connecting assembly; 51. a connecting plate; 52. an elastic strip; 6. a note; 7. a butt strap; 8. and (4) calibration.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention is described in detail with reference to the accompanying drawings and the detailed description.

As shown in fig. 1-4, the VEGF antigen ultrafiltration centrifugal tube includes a tube body 1, a tube cap 2, an inner tube with molecular weight cut-off 3, and a connecting assembly 5, wherein the inner tube with molecular weight cut-off 3 is a tapered tube made of polyethylene, a filtering membrane embedded in the inner tube with molecular weight cut-off 3 is made of polyethersulfone, and the arrangement of the inner tube with molecular weight cut-off 3 is the prior art and is not described herein again; the pipe body 1 is internally clamped with the molecular weight cutoff inner pipe 3 through the fixing ring 4, and the inner side surface of the fixing ring 4 is matched with the outer side surface of the molecular weight cutoff inner pipe 3, so that the molecular weight cutoff inner pipe 3 is well supported. The tube cover 2 comprises a cover body 21 and a reinforcing mechanism 22; the cover body 21 is an annular cylinder body; the bottom end of the cover body 21 is inserted with the top end of the inner cavity of the tube body 1; the reinforcing mechanism 22 is fixedly arranged at the bottom end of the inner cavity of the cover body 21; the reinforcing mechanism 22 is abutted against the top surface of the molecular weight cutoff inner tube 3; lid 21 and body 1 can dismantle through coupling assembling 5 and be connected, and 21 sides of lid are pasted and are had memo 6, are convenient for mark experimental item or experimental object, avoid appearing the mark and clearly lead to the experiment unable go on.

Furthermore, the mark volume scale 8 is vertically arranged on the outer side surface of the molecular weight cutoff inner tube 3, so that the experimenter can observe the injection volume conveniently.

In a further optimized scheme, the reinforcing mechanism 22 comprises a fixing block 221; a screw hole is formed in the center of the fixing block 221; a blocking plug 222 is screwed in the screw hole, the blocking plug 222 is screwed into the screw hole to realize integral sealing, and the inside of the blocking plug 222 can be filled with a filtering stock solution through an injector after being screwed out; the side and bottom surfaces of the fixing block 221 are provided with a plurality of pushing holes 223 and a plurality of pressing holes 224; the pushing holes 223 and the pressing holes 224 are arranged in a one-to-one correspondence and are communicated with each other; the inner cavity of the push hole 223 and the inner cavity of the press hole 224 are respectively provided with a material pressing component; the material pressing component is abutted against the top surface of the molecular weight cutoff inner tube 3.

In a further optimized scheme, the pressing assembly comprises a first wedge 225 and a second wedge 226; the first wedge 225 is slidably connected with the press hole 224; the second wedge pillar 226 is slidably connected with the pushing hole 223; one end of the first wedge 225 and one end of the second wedge 226 are respectively provided with an inclined plane; the two inclined planes are matched and correspondingly arranged; a sealing layer 227 is fixedly connected to the bottom surface of the pressure hole 224; the bottom surface of the first wedge 225 abuts the top surface of the sealing layer 227; the bottom surface of the sealing layer 227 is abutted against the top surface of the molecular weight cut-off inner tube 3, the sealing layer 227 is a high-elasticity rubber layer which can deform along with the extrusion of the second wedge 226, and the sealing layer 227 can isolate the inner cavity of the tube body 1 from the cover body 21; the top surface of the molecular weight cutoff inner pipe 3 is fixedly connected with a plurality of butt straps 7; the top surface of the access board 7 is arranged in a manner of being abutted to the sealing layer 227, and the pressure of the first wedge column 225 can be transmitted to the top surface of the molecular weight cutoff inner tube 3 through the access board 7, so that the molecular weight cutoff inner tube 3 can be firmly fixed on the fixing ring 4 in the centrifugal process. A gap is formed between the other end of the second wedge post 226 and the side surface of the stopper 222, so that the second wedge post 226 can slide in the pushing hole 223 in a space; meanwhile, the second wedge 226 is made of stainless steel, and has a high density, so that the pushing force applied to the first wedge 225 is higher under the centrifugal condition.

In a further optimized scheme, the connecting component 5 can connect the pipe body 1 and the pipe cover 2 into a whole and comprises two connecting plates 51; two connecting plates 51 one end respectively with body 1 lateral surface and lid 21 lateral surface fixed connection, two connecting plates 51's the other end passes through elasticity strip 52 fixed connection, can enough guarantee that the tube cap 2 does not thoroughly separate with body 1 after extracting, and then lead to lid 21 to lose or the wrong cross contamination that appears of lid, can also be convenient for lid 21 and body 1's lock through the tensile retraction of elasticity strip 52.

The working process of the embodiment is as follows:

when a VEGF antigen ultrafiltration centrifugal experiment needs to be carried out, a filtering stock solution is injected into the molecular weight cut-off inner tube 3 through a screw hole, and the injection volume is determined through the scale 8. Then, the stop plug 222 is screwed into the screw hole for sealing; will the utility model discloses put into centrifuge (the attached drawing does not show), centrifuge is high-speed rotatory, make second wedge post 226 in pushing away the hole 223 remove to the direction that is close to first wedge post 225, and extrude first wedge post 225 lapse through the inclined plane, when first wedge post 225 lapse goes out pressure hole 224, can further extrude the sealing layer 227 parcel live the bottom of first wedge post 225 and inconsistent with attachment strap 7 top surface, guaranteed the stability of trapped molecular weight inner tube 3 operation process in body 1.

In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are merely for convenience of description of the present invention, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.

The above-mentioned embodiments are only intended to describe the preferred embodiments of the present invention, but not to limit the scope of the present invention, and those skilled in the art should also be able to make various modifications and improvements to the technical solution of the present invention without departing from the spirit of the present invention, and all such modifications and improvements are intended to fall within the scope of the present invention as defined in the appended claims.

Claims

1. A VEGF antigen ultrafiltration centrifugal tube is characterized by comprising a tube body (1), a tube cover (2), a molecular weight cut-off inner tube (3) and a connecting assembly (5); the tube body (1) is clamped with the molecular weight cutoff inner tube (3) through a fixing ring (4); the pipe cover (2) comprises a cover body (21) and a reinforcing mechanism (22); the cover body (21) is an annular cylinder body; the bottom end of the cover body (21) is inserted into the top end of the inner cavity of the tube body (1); the reinforcing mechanism (22) is fixedly arranged at the bottom end of the inner cavity of the cover body (21); the reinforcing mechanism (22) is abutted against the top surface of the molecular weight cut-off inner pipe (3); the cover body (21) is detachably connected with the pipe body (1) through the connecting component (5).

2. The VEGF antigen ultrafiltration centrifuge tube of claim 1, wherein: the reinforcing mechanism (22) comprises a fixing block (221); a screw hole is formed in the center of the fixing block (221); a stop plug (222) is screwed in the screw hole; the side surface and the bottom surface of the fixed block (221) are provided with a plurality of push holes (223) and a plurality of press holes (224); the push holes (223) and the press holes (224) are arranged in a one-to-one correspondence manner and are communicated with each other; the inner cavity of the push hole (223) and the inner cavity of the press hole (224) are respectively provided with a material pressing component; the material pressing component is abutted to the top surface of the molecular weight cut-off inner tube (3).

3. The VEGF antigen ultrafiltration centrifuge tube of claim 2, wherein: the swaging assembly comprises a first wedge (225) and a second wedge (226); one end of the first wedge column (225) and one end of the second wedge column (226) are respectively provided with an inclined surface; the first wedge column (225) is connected with the pressing hole (224) in a sliding mode; the second wedge column (226) is in sliding connection with the push hole (223); the two inclined planes are matched and correspondingly arranged; a sealing layer (227) is fixedly connected to the bottom surface of the pressure hole (224); the bottom surface of the first wedge column (225) is abutted against the top surface of the sealing layer (227); the bottom surface of the sealing layer (227) is abutted against the top surface of the molecular weight cut-off inner pipe (3); the other end of the second wedge column (226) is detachably connected with the side surface of the blocking plug (222).

4. The VEGF antigen ultrafiltration centrifuge tube of claim 3, wherein: the molecular weight cut-off inner pipe (3) is a polyethylene conical pipe; the top surface of the molecular weight cutoff inner pipe (3) is fixedly connected with a plurality of butt straps (7); the top surface of the butt strap (7) is arranged in a manner of being abutted to the sealing layer (227).

5. The VEGF antigen ultrafiltration centrifuge tube of claim 1, wherein: the connecting assembly (5) comprises two connecting plates (51); two connecting plate (51) one end respectively with body (1) lateral surface with lid (21) lateral surface fixed connection, two the other end of connecting plate (51) is through elasticity strip (52) fixed connection.

6. The VEGF antigen ultrafiltration centrifuge tube of claim 5, wherein: the inner side surface of the fixing ring (4) is matched with the outer side surface of the molecular weight cut-off inner tube (3).

7. The VEGF antigen ultrafiltration centrifuge tube of claim 1, wherein: the side surface of the cover body (21) is pasted with a note (6).

8. The VEGF antigen ultrafiltration centrifuge tube of claim 3, wherein: the second wedge column (226) is made of stainless steel; the sealing layer (227) is a highly elastic rubber layer.