CN217092187U - Series-parallel connection chromatography device - Google Patents
Series-parallel connection chromatography device Download PDFInfo
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- CN217092187U CN217092187U CN202220728638.7U CN202220728638U CN217092187U CN 217092187 U CN217092187 U CN 217092187U CN 202220728638 U CN202220728638 U CN 202220728638U CN 217092187 U CN217092187 U CN 217092187U
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Abstract
The utility model discloses a series-parallel connection chromatography device, which is characterized by comprising a fixed bracket, a multi-channel peristaltic pump arranged on the fixed bracket, and chromatography pipelines which are communicated with the multi-channel peristaltic pump and are mutually connected in parallel; each chromatography pipeline is provided with at least three chromatography columns which are connected in series and are connected end to end, macroporous adsorption resin is filled in each chromatography column, and each chromatography column is fixed on the fixed support through a detachable hoop; the utility model has the advantages that a plurality of chromatographic columns are used in series, different fillers are filled in the chromatographic columns in series connection to adsorb different chemical components, and a plurality of impurities can be removed by one-time operation, thereby improving the purification efficiency; meanwhile, a multi-channel peristaltic pump is arranged at the starting end, so that a plurality of purification threads can be simultaneously and parallelly arranged, the multi-channel peristaltic pump has the characteristic of high flux, two groups of different samples can be separated and purified, and the time is saved.
Description
Technical Field
The utility model belongs to the technical field of biomedical instrument, especially, relate to a series-parallel connection chromatography device.
Background
The traditional Chinese medicine has complex components, wherein chemical components such as alkaloid, volatile oil, polysaccharide, trace elements and the like have obvious medical effects, and give to flattery is formed by grease, protein, organic acid, pigment and the like which have fewer medicinal components at present, so that the effective components are required to be separated and purified.
The common separation methods include ultra-near extraction, membrane extraction and separation, flocculation separation, and macroporous adsorbent resin adsorption. Supercritical fluid extraction method is to extract effective components from Chinese medicinal materials with supercritical fluid as extractant and separate the effective components from liquid or solid. The principle is that a supercritical fluid extracts domestic effective components from a target object at a temperature higher than the critical temperature and pressure, and when the temperature is returned to normal pressure and normal temperature, the components dissolved in the fluid are immediately separated from the gaseous fluid in a liquid state dissolved in an absorption liquid. The membrane extraction and separation method is to utilize the molecular weight difference of chemical components to reach the separation aim, and in the application of Chinese medicine, the membrane extraction and separation method is mainly to filter out bacteria, particles, macromolecular impurities (colloid, tannin, protein, polysaccharide), etc. or decolor. The flocculation separation of Chinese medicine is to add flocculant into water extract of Chinese medicine and to make many unstable particles, such as protein, manganese liquid, saddle, etc. form floc to settle through the adsorption, bridging and flocculation of flocculant and the coagulation of inorganic salt electrolyte particles and surface charges, and to filter and purify. Nowadays, the macroporous adsorption resin method is widely applied to the separation and refining of the active ingredients of the traditional Chinese medicine. The technology is widely used for purifying glycosides, flavonoids, alkaloids and polysaccharides. However, the supercritical fluid extraction method has high equipment cost, so that the proportion of equipment depreciation cost in the product cost is overlarge, and the equipment is difficult to clean when the product is replaced. Membrane fouling in membrane extraction separation processes requires periodic replacement. Flocculation separation techniques are also not applicable to a small number of drugs.
If the macroporous adsorption resin is statically adsorbed by using a soaking method, the adsorption efficiency is greatly limited in terms of contact area, and the efficiency can be obviously improved by using a chromatographic column method for dynamic adsorption. However, for a complex sample of traditional Chinese medicine, the separation and purification effect may not be achieved by one resin or single separation, the whole process can only be operated intermittently, and cannot be operated continuously, and the procedure is complicated and time-consuming.
Disclosure of Invention
The utility model aims at providing a connection in series-parallel chromatography device can reach the increase column height on the one hand, improves separation efficiency's effect, and on the other hand can also load the resin of different grade type for adsorb multiple impurity, solve for liquid sample cross the chromatography column consuming time, inefficiency, problem with high costs.
The technical scheme of the utility model is that: a series-parallel connection chromatography device comprises a fixed support, a multi-channel peristaltic pump arranged on the fixed support, and chromatography pipelines which are communicated with the multi-channel peristaltic pump and are connected in parallel;
at least three chromatographic columns which are connected in series and are connected end to end are arranged on each chromatographic pipeline, macroporous adsorption resin is filled in each chromatographic column, and each chromatographic column is fixed on the fixed support through a detachable clamp.
As a preferred technical scheme, the detachable clamp comprises two semicircular clamping blocks and a butterfly screw, wherein the two semicircular clamping blocks are used for clamping the chromatographic column, one end parts of the two semicircular clamping blocks are hinged with each other, and the butterfly screw is used for fixing the other end parts of the two semicircular clamping blocks; meanwhile, one semicircular clamping block is fixed on the fixed bracket.
As an optimized technical scheme, the fixing support adopts an aluminum alloy frame, and comprises at least four stand columns, four main cross beams and four secondary cross beams, wherein the four main cross beams are respectively and transversely arranged at the top ends of every two stand columns, and the four secondary cross beams are respectively and transversely arranged at the middle sections of every two stand columns.
As the preferred technical scheme, the detachable clamps are all fixed on the inner side wall of the main cross beam.
As a preferred technical scheme, a fixed plate is arranged on the secondary cross beam positioned on the right side, and the multi-channel peristaltic pump is arranged on the fixed plate.
As a preferred technical scheme, the multi-channel peristaltic pump comprises a pump body fixed on the fixing plate and two pump heads connected with the pump body; each pump head is correspondingly connected with a chromatography pipeline, and each chromatography pipeline is provided with three chromatography columns which are connected in series and are connected end to end.
As a preferred technical scheme, macroporous absorption resins with different types are filled in chromatographic columns which are positioned on the same chromatographic pipeline and are mutually connected in series.
The utility model has the advantages that:
1. the utility model discloses a plurality of chromatography columns establish ties and use, and pack different fillers in the chromatography column of establishing ties, adsorb different chemical composition, and multiple impurity can be got rid of in operation, improves purification efficiency, has the efficient advantage.
2. The utility model discloses a be equipped with the multichannel peristaltic pump at the top, can realize many parallel purification threads simultaneously, have the characteristics of high flux, the sample of two sets of differences also can separate the purification, saves time.
3. The utility model discloses because of adopting the series-parallel connection device, to activation and regeneration convenient and fast of resin packing, and the device has extensive usage in fields such as traditional chinese medicine chemical composition purification, decoloration, deproteinization.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required to be used in the description of the embodiments are briefly introduced below, the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
The invention will be further described with reference to the following drawings and examples:
FIG. 1 is a schematic diagram of the device of the present invention;
FIG. 2 is a schematic structural view of the present invention;
fig. 3 is a schematic structural view of the detachable clamp of the present invention;
wherein: 1, fixing a bracket, 11 columns, 12 main beams, 13 secondary beams and 14 fixing plates;
2, a multi-channel peristaltic pump, 21 a pump body and 22 a pump head;
3, a chromatography pipeline, 4 chromatography columns and 5 macroporous adsorption resin;
6 detachable hoop, 61 semicircular fixture block, 62 butterfly screw.
Detailed Description
The above-described scheme is further illustrated below with reference to specific examples. It should be understood that these examples are for illustrative purposes and are not intended to limit the scope of the present invention. The conditions used in the examples may be further adjusted according to the conditions of the particular manufacturer, and the conditions not specified are generally the conditions in routine experiments.
Example 1: referring to fig. 1 to 3, the series-parallel chromatography device of the present invention mainly comprises a fixed support 1, a multi-channel peristaltic pump 2 disposed on the fixed support 1, and a chromatography pipeline 3 communicated with the multi-channel peristaltic pump 2 and connected in parallel with each other; three chromatographic columns 4 which are connected in series and are connected end to end are arranged on each chromatographic pipeline 3, macroporous adsorption resin 5 is filled in each chromatographic column 4, and each chromatographic column 4 is fixed on the fixed support 1 through a detachable clamp 6.
The detachable clamp 6 in this embodiment 1 includes two semicircular blocks 61 for clamping the chromatography column 4, one end of each of which is hinged to each other, and a thumb screw 62 for fixing the other end of each of the two semicircular blocks 61; and one semicircular clamping block 61 is fixed on the fixed bracket 1.
The fixing bracket 1 in this embodiment 1 is an aluminum alloy frame, and includes at least four upright posts 11, four main beams 12 respectively transversely disposed at the top ends of every two upright posts 11, and four secondary beams 13 respectively transversely disposed at the middle sections of every two upright posts 11; wherein the detachable clamps 6 are fixed on the inner side wall of the main beam 12, the secondary beam 13 on the right side is provided with a fixing plate 14, and the multi-channel peristaltic pump 2 is arranged on the fixing plate 14.
The multi-channel peristaltic pump 2 in the embodiment 1 includes a pump body 21 fixed on the fixing plate 14, and two pump heads 22 connected to the pump body 21; each pump head 22 is correspondingly connected with one chromatography pipeline 3, three chromatography columns 4 which are connected in series are arranged on each chromatography pipeline 3, the chromatography columns are connected end to end, and macroporous adsorption resins 5 with different models are filled in the chromatography columns 4 which are arranged on the same chromatography pipeline 3 and are connected in series.
When the sample liquid is pumped from the sample inlet end of the multi-channel peristaltic pump 2, the multi-channel peristaltic pump 2 is connected with the upper end of the chromatographic column 4, the lower end of the chromatographic column 4 is connected with the upper end of the next chromatographic column 4, the chromatographic columns are sequentially connected end to end, and the lower end outlet of the third chromatographic column 4 is a sample liquid outlet. Taking the activated anion resin as an example, 2 times of column volume of ethanol is pumped through the sample inlet to elute the resin, 5% HCl is used to elute the resin for 2 times of column volume, pure water is used to elute the resin to be neutral, and finally 2 times of column volume is eluted by 2% -4% NaoH solution and is eluted by pure water to be neutral. In the sample loading process, the stock solution can be subjected to different resins to remove impurities, such as deproteinization, pigment removal and the like, and the flow-through solution can be collected at the sample inlet and outlet. If the component absorbed by the elution resin is needed, the elution liquid can be pumped in again.
The above embodiments are only for illustrating the technical concept and features of the present invention, and the purpose of the embodiments is to enable people skilled in the art to understand the contents of the present invention and to implement the present invention, which cannot limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered by the protection scope of the present invention.
Claims (7)
1. A series-parallel connection chromatography device is characterized by comprising a fixed support, a multi-channel peristaltic pump arranged on the fixed support, and chromatography pipelines which are communicated with the multi-channel peristaltic pump and are connected in parallel;
at least three chromatographic columns which are connected in series and are connected end to end are arranged on each chromatographic pipeline, macroporous adsorption resin is filled in each chromatographic column, and each chromatographic column is fixed on the fixed support through a detachable clamp.
2. The series-parallel chromatography device as claimed in claim 1, wherein the detachable clamp comprises two semicircular blocks for clamping the chromatography column, one end of each semicircular block being hinged to the other end of each semicircular block, and a thumb screw for fixing the other ends of the semicircular blocks; meanwhile, one semicircular clamping block is fixed on the fixed bracket.
3. The device of claim 1, wherein the fixing frame is an aluminum alloy frame, and comprises at least four columns, four main beams respectively transversely disposed at the top ends of two columns, and four secondary beams respectively transversely disposed at the middle sections of two columns.
4. The series-parallel chromatography device of claim 3, wherein the detachable clamps are fixed on the inner side wall of the main beam.
5. The series-parallel chromatography device according to claim 3, wherein a fixing plate is provided on the secondary beam on the right side, and the multichannel peristaltic pump is provided on the fixing plate.
6. The series-parallel chromatography device of claim 5, wherein the multichannel peristaltic pump comprises a pump body fixed on the fixed plate, and two pump heads connected with the pump body; each pump head is correspondingly connected with a chromatography pipeline, and each chromatography pipeline is provided with three chromatography columns which are connected in series and are connected end to end.
7. The series-parallel chromatography device of claim 1, wherein different types of macroporous adsorbent resins are filled in the chromatographic columns which are positioned on the same chromatography pipeline and are connected in series.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220728638.7U CN217092187U (en) | 2022-03-30 | 2022-03-30 | Series-parallel connection chromatography device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220728638.7U CN217092187U (en) | 2022-03-30 | 2022-03-30 | Series-parallel connection chromatography device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217092187U true CN217092187U (en) | 2022-08-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220728638.7U Active CN217092187U (en) | 2022-03-30 | 2022-03-30 | Series-parallel connection chromatography device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217092187U (en) |
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2022
- 2022-03-30 CN CN202220728638.7U patent/CN217092187U/en active Active
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