CN217083129U - Drying mechanism is used in production of stimulus response type cell culture container - Google Patents
Drying mechanism is used in production of stimulus response type cell culture container Download PDFInfo
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- CN217083129U CN217083129U CN202220715610.XU CN202220715610U CN217083129U CN 217083129 U CN217083129 U CN 217083129U CN 202220715610 U CN202220715610 U CN 202220715610U CN 217083129 U CN217083129 U CN 217083129U
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Abstract
The utility model discloses a drying mechanism for production of stimulus response type cell culture containers, which relates to the technical field of biological medicine, and comprises a shell and a PLC (programmable logic controller), wherein a heating component placing cavity and a drying cavity are arranged in the shell, the heating component placing cavity is communicated with the drying cavity through a heating hole, a heating component is arranged in the heating component placing cavity, and the heating component adopts a PTC (positive temperature coefficient) electric heater; the drying cavity is internally provided with a ventilation assembly and a temperature sensor, the ventilation assembly comprises a fan fixing frame and a fan arranged on the fan fixing frame, the fan fixing frame is arranged on the shell, and the PLC is connected with the PTC electric heater, the temperature sensor and the fan.
Description
Technical Field
The utility model relates to a biological medicine technical field especially relates to a stoving mechanism is used in production of stimulus response type cell culture container.
Background
For adherently growing cells, the desorption and transfer of the cells from the wall surface of a cell culture plate, a culture bottle or a culture dish is the most basic and one of the most frequently involved experimental operations in cell engineering and tissue engineering. The currently used method of cell detachment is trypsin digestion. However, besides being able to digest intercellular matrix proteins, pancreatin also digests cell membrane proteins over a long period of time, causing damage to cells. Therefore, when cells are digested with pancreatin, it is necessary to strictly control parameters such as the concentration of pancreatin, the action time, the temperature, and the number of washes. Under the conditions of weak alkalinity and 37 ℃, the digestion capacity of the pancreatin solution is the strongest, but the integrity of the membrane structure and the function of cells is easily damaged by slight negligence during operation, so that the activity of the cells is reduced, and even the cells are dead.
In recent years, stimulus-responsive polymer materials (also referred to as smart polymer materials, sensitive polymers, and environmentally sensitive polymers), particularly temperature-responsive surfaces represented by PNIPAM, have been widely used in the fields of cell culture, tissue engineering, and the like. Poly (N-isopropyl acrylamide, PNIPAM) is a most common temperature-sensitive high polymer material which is relatively easy to prepare, and is modified on the surface of a material (such as a cell culture apparatus), and hydrophilic-hydrophobic conversion of the surface is realized through temperature stimulation, so that the cell culture apparatus with temperature response characteristic is obtained. When the cell culture apparatus is used for cell culture, cells can be separated from the wall surface of the cell culture apparatus simply by raising or lowering the ambient temperature, and the digestion and the cell purging by pancreatin can be avoided. Compared with the traditional enzymolysis cell desorption mode, the stimulation response type cell culture instrument provides a means with high efficiency, no harm and no need of adding chemical reagents for the desorption and transfer of cells. According to research and research, only the Japan CellSeed company can realize the commercialization of the temperature-sensitive cell culture apparatus at present, but the temperature-sensitive cell culture apparatus produced by the CellSeed can realize the controllable desorption and attachment of cells, but also has the problems of complex preparation process, long preparation period, small production quantity, poor use stability, small application range of base materials and the like. A drying mechanism is needed in the production of the stimulus-responsive cell culture container, and the conventional drying mechanism has the defects of poor drying effect, inconvenience in operation and the like. Therefore, it is very necessary to design a special drying mechanism for producing temperature-sensitive cell culture apparatus.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem, the utility model provides an amazing response type cell culture container production is with stoving mechanism, after wasing amazing response type cell culture container breaks away from the surface of water, starts ventilation components and heating element, carries out drying process to wasing amazing response type cell culture container, closes ventilation components and heating element after drying 0.5-12h, accomplishes stoving work.
The utility model aims at realizing through the following technical scheme:
the utility model provides a production of stimulus response type cell culture container is with stoving mechanism, includes shell body and PLC controller, its characterized in that: a heating assembly placing cavity and a drying cavity are arranged in the shell of the shell, the heating assembly placing cavity is communicated with the drying cavity through a heating hole, a heating assembly is arranged in the heating assembly placing cavity, and the heating assembly adopts a PTC electric heater; the drying cavity is internally provided with a ventilation assembly and a temperature sensor, the ventilation assembly comprises a fan fixing frame and a fan arranged on the fan fixing frame, the fan fixing frame is arranged on the shell, and the PLC is connected with the PTC electric heater, the temperature sensor and the fan.
Preferably, the ventilation assembly is disposed above the drying chamber.
Preferably, the fan mount includes fixed riser and fixed diaphragm, fixed riser and fixed diaphragm and casing shell fixed connection, be provided with fan installation swash plate on fixed riser and the fixed diaphragm, fixed riser, fixed diaphragm and fan installation swash plate are triangle-shaped structure distribution.
Preferably, the fixed vertical plate is provided with an air inlet.
Preferably, the fan installation sloping plate is provided with a plurality of fans.
Preferably, the included angle between the fan installation inclined plate and the vertical direction is 15-45 degrees.
Preferably, universal wheels are arranged below the shell of the shell.
Preferably, detachable door panels are arranged on the left side and the right side of the shell.
Preferably, the front end of the shell of the drying cavity is also provided with an observation window.
The working principle is as follows: after the cleaned stimulus-responsive cell culture container is separated from the water surface, starting the ventilation assembly and the heating assembly to dry the cleaned stimulus-responsive cell culture container, detecting the temperature in the drying cavity by the temperature sensor, and maintaining the temperature in the drying cavity to be 30-80 ℃ by the cooperation of the PLC, the temperature sensor and the PTC electric heater.
The beneficial effects of this technical scheme are as follows:
the utility model provides a production of stimulus response type cell culture container is with stoving mechanism, after the stimulus response type cell culture container that washs breaks away from the surface of water, start ventilation subassembly and heating element, carry out drying process to the stimulus response type cell culture container that washs, close ventilation subassembly and heating element after drying 0.5-12h, accomplish stoving work.
Two, the utility model provides a stimulus response type cell culture container production is with stoving mechanism, the wind after the heating flows into the stoving chamber through the heated orifice, and ventilation assembly sets up and can make stoving intracavity temperature more even in stoving chamber upper end.
Thirdly, the utility model provides a drying mechanism is used in production of stimulus response type cell culture container, fan installation swash plate and stimulus response type cell culture container hold the device and be in same horizontal plane, and the fan installation swash plate is 15-45 with the contained angle of vertical direction for stimulus response type cell culture container drying speed is faster.
Four, the utility model provides a drying mechanism is used in production of stimulus response type cell culture container, the setting up of universal wheel makes drying mechanism remove more convenient, and the setting up of dismantling the door plant makes heating element and ventilation module change more convenient, and the stoving effect is conveniently looked over in setting up of observation window, in time takes out the stimulus response type cell culture container that dries.
Drawings
The invention will be described in further detail with reference to the following description taken in conjunction with the accompanying drawings and detailed description, in which:
fig. 1 is a schematic structural view of the present invention;
fig. 2 is a schematic structural diagram of a heating assembly according to the present invention;
fig. 3 is a schematic structural view of the ventilation assembly of the present invention;
FIG. 4 is a control schematic diagram of the present invention;
the labels in the figure are: 510. a housing shell; 511. a universal wheel; 512. a door panel; 520. a heating assembly placing cavity; 530. a drying cavity; 540. heating the hole; 550. a heating assembly; 560. a ventilation assembly; 561. a fan fixing frame; 562. a fan; 563. fixing a vertical plate; 564. fixing the transverse plate; 565. the fan is provided with an inclined plate; 566. an air inlet; 570. a temperature sensor; 800. a PLC controller.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
As shown in fig. 1-3, a drying mechanism for production of a stimulus-responsive cell culture container comprises a housing shell 510 and a PLC controller 800, wherein a heating element placing cavity 520 and a drying cavity 530 are arranged in the housing shell 510, the heating element placing cavity 520 is communicated with the drying cavity 530 through a heating hole 540, a heating element 550 is arranged in the heating element placing cavity 520, and the heating element 550 adopts a PTC electric heater; the voltage of the PTC electric heater is 220V; a ventilation assembly 560 and a temperature sensor 570 are arranged in the drying cavity 530, the ventilation assembly 560 comprises a fan fixing frame 561 and a fan 562 arranged on the fan fixing frame 561, the fan fixing frame 561 is arranged on the shell body 510, the temperature transmitter 570 adopts a KZW-DPTHT HART protocol temperature transmitter, and the PLC 800 is connected with the PTC electric heater, the temperature sensor and the fan 562. And after the cleaned stimulus-responsive cell culture container is separated from the water surface, starting the ventilation assembly 560 and the heating assembly 550, drying the cleaned stimulus-responsive cell culture container, and after drying for 0.5-12h, closing the ventilation assembly 560 and the heating assembly 550 to finish the drying work.
Wherein the ventilation assembly 560 is disposed above the drying chamber 530. The heated air flows into the drying chamber 530 through the heating holes 540, and the temperature in the drying chamber 530 is more uniform due to the arrangement of the ventilation assembly 560 at the upper end of the drying chamber 530, which is beneficial to rapid drying.
The fan fixing frame 561 comprises a fixing vertical plate 563 and a fixing transverse plate 564, the fixing vertical plate 563 and the fixing transverse plate 564 are fixedly connected with the shell 510, fan installation inclined plates 565 are arranged on the fixing vertical plate 563 and the fixing transverse plate 564, and the fixing vertical plate 563, the fixing transverse plate 564 and the fan installation inclined plates 565 are distributed in a triangular structure.
Wherein, air inlet 566 is arranged on the fixed riser 563.
Wherein, a plurality of fans 562 are arranged on the fan installation inclined plate 565.
Wherein, the included angle between the fan installation inclined plate 565 and the vertical direction is 15-45 degrees. The inclined fan installation plate 565 is positioned on the same horizontal plane with the containing device of the stimulus-responsive cell culture container, and the included angle between the inclined fan installation plate 565 and the vertical direction is 15-45 degrees, so that the drying speed of the stimulus-responsive cell culture container is higher.
Wherein, a universal wheel 511 is arranged below the shell 510.
Wherein, detachable door panels 512 are arranged on the left and right sides of the shell casing 510.
Wherein, the front end of the casing 510 at the drying cavity 530 is further provided with an observation window. The setting of universal wheel 511 makes the stoving mechanism remove more conveniently, and the setting of dismantling door plant 512 makes heating element 550 and ventilation assembly 560 change more conveniently, and the setting of observation window conveniently looks over the stoving effect, in time takes out the stimulus response type cell culture container who dries.
The working principle is as follows: after the cleaned stimulus-responsive cell culture container is separated from the water surface, the ventilation assembly 560 and the heating assembly 550 are started to dry the cleaned stimulus-responsive cell culture container, the temperature sensor detects the temperature in the drying cavity 530, and the temperature in the drying cavity 530 is maintained to be 30-80 ℃ through the cooperation of the PLC 800, the temperature sensor and the PTC electric heater.
In conclusion, after the ordinary skilled in the art reads the document of the present invention, the technical solution and technical idea of the present invention do not need creative mental labor and make other various corresponding transformation schemes, which all belong to the protection scope of the present invention.
Claims (9)
1. A drying mechanism for production of stimulus response type cell culture containers comprises a shell (510) and a PLC (800), and is characterized in that: a heating assembly placing cavity (520) and a drying cavity (530) are arranged in the shell (510), the heating assembly placing cavity (520) is communicated with the drying cavity (530) through a heating hole (540), a heating assembly (550) is arranged in the heating assembly placing cavity (520), and the heating assembly (550) adopts a PTC electric heater; the drying chamber (530) is internally provided with a ventilation assembly (560) and a temperature sensor (570), the ventilation assembly (560) comprises a fan fixing frame (561) and a fan (562) arranged on the fan fixing frame (561), the fan fixing frame (561) is arranged on the shell body shell (510), and the PLC (800) is connected with the PTC electric heater, the temperature sensor (570) and the fan (562).
2. The drying mechanism for stimulus-responsive cell culture vessel production according to claim 1, wherein: the ventilation assembly (560) is disposed above the drying chamber (530).
3. The drying mechanism for stimulus-responsive cell culture vessel production according to claim 2, wherein: fan mount (561) are including fixed riser (563) and fixed diaphragm (564), fixed riser (563) and fixed diaphragm (564) and casing shell (510) fixed connection, be provided with fan installation swash plate (565) on fixed riser (563) and the fixed diaphragm (564), fixed riser (563), fixed diaphragm (564) and fan installation swash plate (565) are the distribution of triangle-shaped structure.
4. The drying mechanism for stimulus-responsive cell culture vessel production according to claim 3, wherein: an air inlet (566) is arranged on the fixed vertical plate (563).
5. The drying mechanism for stimulus-responsive cell culture vessel production according to claim 4, wherein: the fan installation inclined plate (565) is provided with a plurality of fans (562).
6. The drying mechanism for stimulus-responsive cell culture vessel production according to claim 5, wherein: the included angle between the fan installation inclined plate (565) and the vertical direction is 15-45 degrees.
7. The drying mechanism for the production of stimulus-responsive cell culture vessels of claim 6, wherein: and universal wheels (511) are arranged below the shell (510).
8. The drying mechanism for stimulus-responsive cell culture vessel production according to claim 7, wherein: the left side and the right side of the shell body shell (510) are provided with detachable door plates (512).
9. The drying mechanism for stimulus-responsive cell culture vessel production according to claim 8, wherein: the front end of the shell (510) at the drying cavity (530) is also provided with an observation window.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220715610.XU CN217083129U (en) | 2022-03-30 | 2022-03-30 | Drying mechanism is used in production of stimulus response type cell culture container |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220715610.XU CN217083129U (en) | 2022-03-30 | 2022-03-30 | Drying mechanism is used in production of stimulus response type cell culture container |
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| Publication Number | Publication Date |
|---|---|
| CN217083129U true CN217083129U (en) | 2022-07-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220715610.XU Active CN217083129U (en) | 2022-03-30 | 2022-03-30 | Drying mechanism is used in production of stimulus response type cell culture container |
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| CN (1) | CN217083129U (en) |
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- 2022-03-30 CN CN202220715610.XU patent/CN217083129U/en active Active
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