CN217059916U

CN217059916U - Immobilized enzyme molecularly imprinted photonic crystal detection device

Info

Publication number: CN217059916U
Application number: CN202121271552.8U
Authority: CN
Inventors: 范晓莹; 郭明
Original assignee: Zhejiang A&F University ZAFU
Current assignee: Zhejiang A&F University ZAFU
Priority date: 2021-06-08
Filing date: 2021-06-08
Publication date: 2022-07-26
Anticipated expiration: 2031-06-08

Abstract

The utility model discloses a molecular imprinting photonic crystal detection device of immobilized enzyme, which comprises a green light emitting device, a container, an integrating sphere, a fiber spectrometer and a molecular imprinting photonic crystal; the container is arranged in the integrating sphere, the green light emitting device is arranged on one side of the container, the molecular imprinting photonic crystal is arranged in the container and fixed through a tenon-connection fixture above the container, and the output end of the integrating sphere is connected with the optical fiber spectrometer. The device of the utility model integrates the functions of specificity identification, degradation and detection, and has the characteristics of convenient operation.

Description

Immobilized enzyme molecularly imprinted photonic crystal detection device

Technical Field

The utility model relates to a photonic crystal technology field especially relates to an immobilized enzyme's molecular imprinting photonic crystal detection device.

Background

The existing detection modes comprise a titration method, a single-scanning polarography method and a gas chromatography/mass spectrometry method, when the GC/MS is used for analyzing the bisphenol-A, the-OH at two ends of the bisphenol-A is derivatized into O-Si (CH3)3, so that the detection limit of the analysis can be improved, although the derivatization reaction of the bisphenol-A is rapid and easy to realize, a series of defects still exist when the derivatization method is used for analyzing, ions for quantification are easily interfered by silicide, and the bisphenol-A possibly exists at the same time, and is not very stable after derivatization. Meanwhile, both LC/MS and GC/MS require sample pretreatment and are relatively troublesome. At present, the research progress of the existing immobilized enzyme sensing device and photoelectric sensing device is difficult to cover the defect that the immobilized enzyme sensing device does not have high selective recognition while respectively presenting the advantages of light stimulus response and enzyme reaction stimulus response, and the target substance cannot be quickly and accurately detected under complex conditions

In recent years, mesoporous silica has been widely used for immobilization of proteases and other enzymes. The mesoporous silicon material is used as a carrier of the novel adsorption degradation material, and the internal structure of the mesoporous silicon material contains a large number of hydroxyl groups, so that a good condition can be provided for enzyme immobilization; the immobilized enzyme carrier material has the advantages of good stability, high mechanical strength, acid and alkali resistance, long service life, no environmental pollution and the like, so the immobilized enzyme carrier material is an excellent immobilized enzyme carrier material, and the ordered structure of the immobilized enzyme carrier material can convert an enzyme degradation process into a recognizable optical signal, and endows the immobilized enzyme carrier material with a novel immobilized enzyme photoresponse performance. However, the research on the application of photonic crystals to the adsorption and degradation detection of pollutants is still needed.

SUMMERY OF THE UTILITY MODEL

In order to overcome the not enough of above-mentioned prior art, the utility model provides an immobilized enzyme's molecular imprinting photonic crystal detection device, this detection device combines together molecular imprinting, photonic crystal mesoporous silica, immobilized enzyme, makes it possess specific identification performance, improves the discernment adsorption efficiency of photonic crystal to the novel filter of target.

In order to achieve the above object, the utility model adopts the following technical scheme: a molecular imprinting photonic crystal detection device of immobilized enzyme comprises a green light emitting device, a container, an integrating sphere, a fiber spectrometer and a molecular imprinting photonic crystal; the container is arranged in the integrating sphere, the green light emitting device is arranged on one side of the container, the molecular imprinting photonic crystal is arranged in the container and fixed through a tenon-connection fixture above the container, and the output end of the integrating sphere is connected with the optical fiber spectrometer.

Further, the distance between the green light emitting device and the container is 5-6 cm.

Further, the molecularly imprinted photonic crystal is mesoporous silicon.

Further, the mesoporous silicon immobilized enzyme.

Further, the interface of container passes through falcon mode with the integrating sphere and connects.

Compared with the prior art, the beneficial effects of the utility model are that: the utility model discloses a device utilizes the optical characteristic of the three-dimensional macroporous structure of mesoporous silicon to utilize the molecular imprinting structure to modify mesoporous silica and make it obtain specificity recognition function, just the utility model discloses also the medium for immobilized enzyme provides a new thinking, because three-dimensional mesoporous silica's mesopore is adjustable, provides good carrier for the immobilized of macromolecular substance enzyme, the existence of all kinds of immobilized enzyme simultaneously, can the specificity pollutant in the degradation solution, along with the pollutant is degraded in the solution, light passes through photonic crystal chip and changes, lets detection device obtain the ability of the change of pollutant in the dynamic monitoring solution. The device of the utility model integrates the functions of specificity identification, degradation and detection, and has the characteristics of convenient operation.

Drawings

FIG. 1 is a schematic structural diagram of the enzyme-immobilized molecular imprinting photonic crystal detection device of the present invention.

Detailed Description

The invention is further explained below with reference to the drawings and examples.

Fig. 1 is a schematic structural diagram of the enzyme-immobilized molecular imprinting photonic crystal detection apparatus of the present invention, and the molecular imprinting photonic crystal detection apparatus includes a green light emitting device 1, a container 2, an integrating sphere 3, a fiber optic spectrometer 5 and a molecular imprinting photonic crystal 6; container 2 sets up in integrating sphere 3, the interface of container 2 is connected through falcon mode with integrating sphere 3, requires experimental apparatus to accomplish after the solution that awaits measuring of packing into, puts into the black case with the device and detects, avoids getting into other miscellaneous light. The green light emitting device 1 is arranged on one side of the container 3, and as the device is transformed into a common integrating sphere with the diameter of about 11cm, the distance between the green light emitting device 1 and the container 2 is 5-6cm, so that other stray light is prevented from entering. The molecularly imprinted photonic crystal 6 adopted in the utility model is mesoporous silicon, which carries enzyme; the molecular imprinting photonic crystal is arranged in the container 2 and fixed through a tenon-connected clamp above the container 3, and the output end of the integrating sphere 3 is connected with the optical fiber spectrometer 5.

The molecularly imprinted photonic crystal 6 of the utility model is obtained by the following method: BA-15 ordered mesoporous silicon is subjected to sol-gel condensation reaction, silane coupling agent (3-chloropropyltrimethoxysilane) containing chloride ions is used for modifying mesoporous silicon dioxide by a grafting method, and chloride ions are introduced into a mesoporous silicon oxide framework through the sol-gel condensation reaction to obtain the chloride ion modified ordered mesoporous silicon. And then carrying out surface molecular imprinting to enable the surface molecular imprinting to be capable of specifically adsorbing the organic light filter DMP, and finally carrying out immobilized enzyme to obtain the molecular imprinting photonic crystal 6.

And assembling the prepared chip to form a complete detection system, and adding organic light filtering agent sewage into a quartz glass container to immerse the chip. And opening the fiber spectrometer for detection, and analyzing the data.

The utility model discloses molecular imprinting photonic crystal detection device's working process does: the prepared molecularly imprinted photonic crystal is fixed on a clamp of a square container 2, the molecularly imprinted photonic crystal is irradiated by a green light emitting device 1, and the wavelength passing through the photonic crystal is output to a fiber spectrometer 5 from a signal output end 4 after being uniform through the wavelength inside an integrating sphere 3. The utility model discloses utilize the photoelectric characteristic of the molecular imprinting photonic crystal of mesoporous silicon and the specificity discernment nature of molecular imprinting to remove the specificity discernment and adsorb to thereby obtain the degradation process through the change of fiber optic spectrometer 5 with light signal and obtain the degradation data with the signal of telecommunication change.

Examples

BA-15 ordered mesoporous silicon is subjected to sol-gel condensation reaction, silane coupling agent (3-chloropropyltrimethoxysilane) containing chloride ions is used for modifying mesoporous silicon dioxide by a grafting method, and chloride ions are introduced into a mesoporous silicon oxide framework through the sol-gel condensation reaction. Obtaining the ordered mesoporous silicon modified by the chloride ions. And then carrying out surface molecular imprinting to enable the surface molecular imprinting to be capable of specifically adsorbing the organic light filter DMP, and finally carrying out immobilized enzyme to obtain a molecular imprinting photonic crystal which is capable of specifically adsorbing and degrading DMP.

The prepared molecular imprinting photonic crystal is arranged in a container 2, the container 2 is arranged in an integrating sphere 3 through fixing of a falcon clamp above the container 2, and an output end 4 of the integrating sphere 3 is connected with an optical fiber spectrometer 5. Respectively adding organic light filter sewage with DMP concentration of 1 × 10-4mol/L, 1 × 10-3mol/L, 1 × 10-2mol/L and 1 × 10-1mol/L into the container 2 to immerse the molecularly imprinted photonic crystal. The green light emitting device 1 is arranged at a position 5-6cm away from the container 5, the green light emitting device 1 and the fiber spectrometer 4 are opened for detection, the maximum wavelength diffraction peaks are measured to be 546nm, 551nm, 570nm and 578nm respectively, it can be seen that the maximum wavelength diffraction peak can generate red shift along with the increase of DMP concentration, and finally the measured DMP concentration can be reduced by 87.6% on average, so that the method has good sewage detection capability and DMP degradation capability.

Claims

1. The molecular imprinting photonic crystal detection device for immobilized enzyme is characterized in that: the device comprises a green light emitting device (1), a container (2), an integrating sphere (3), a fiber spectrometer (5) and a molecular imprinting photonic crystal (6); the container (2) is arranged in the integrating sphere (3), the green light emitting device (1) is arranged on one side of the container (2), the molecular imprinting photonic crystal (6) is arranged in the container (2) and fixed through a tenon-and-mortise clamp above the container (2), and an output end (4) of the integrating sphere (3) is connected with the optical fiber spectrometer (5).

2. The enzyme-immobilized molecularly imprinted photonic crystal detection device of claim 1, wherein: the distance between the green light emitting device (1) and the container (2) is 5-6 cm.

3. The enzyme-immobilized molecularly imprinted photonic crystal detection device of claim 1, wherein: the molecularly imprinted photonic crystal (6) in the container (2) is mesoporous silicon.

4. The enzyme-immobilized molecularly imprinted photonic crystal detection device of claim 3, wherein: the mesoporous silicon is immobilized and loaded with enzyme.

5. The enzyme-immobilized molecularly imprinted photonic crystal detection device of claim 1, wherein: the interface of the container (2) is connected with the integrating sphere (3) in a falcon connection mode.