CN217049587U

CN217049587U - Embryo cell one-step real-time fluorescence PCR detection kit

Info

Publication number: CN217049587U
Application number: CN202122154328.7U
Authority: CN
Inventors: 杨五宁
Original assignee: Nanjing Youer Biotechnology Development Co ltd
Current assignee: Nanjing Youer Biotechnology Development Co ltd
Priority date: 2021-09-06
Filing date: 2021-09-06
Publication date: 2022-07-26
Anticipated expiration: 2031-09-06

Abstract

The utility model discloses a real-time fluorescence PCR detection kit of embryo cell one-step method, which relates to the embryo cell detection equipment technical field and comprises a test box body, a torsional spring is fixedly connected with one end of a movable column, the other end of the movable column is fixedly connected with a gear, one side of the gear is engaged with a rack plate, the upper end of the rack plate is fixedly provided with a support panel, the upper end of the support panel is fixedly provided with a lifting block, the cover plate pulls a rope through a fixed fixing ring in the pulling process, the movable column is driven to rotate because the rope is wound and connected with the movable column, the torsional spring and the gear are driven to rotate at the same time, the rack plate is driven to move upwards while the gear rotates, namely, the support panel is driven to move along a sliding rod, the lifting block is driven to slide upwards in an inner cavity of a reagent tank, and a part of a reagent bottle can be ejected out, the effect that the operator conveniently took the reagent bottle has been reached.

Description

Embryo cell one-step real-time fluorescence PCR detection kit

Technical Field

The utility model relates to an embryo cell check out test set technical field specifically is an embryo cell one-step method real-time fluorescence PCR detect reagent box.

Background

Embryonic stem cells are a class of cells isolated from early embryos or primitive gonads that have the properties of immortalization, self-renewal and multipotential differentiation in vitro culture. ES cells can be induced to differentiate into almost all cell types in the body, both in vitro and in vivo, and when tested, the test solution is contained in a reagent bottle and then placed in a PCR test kit.

The existing PCR detection kit is stored in a corresponding reagent groove by filling a reagent bottle, when the reagent bottle needs to be taken out, the reagent bottle cannot be taken out conveniently, and the reagent bottle can be damaged in the forced taking-out process, so that liquid flows out and certain loss is caused.

In order to solve the problems, the utility model provides a real-time fluorescence PCR detection kit of embryo cell one-step method.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a real-time fluorescence PCR detection kit of embryo cell one-step method, torsional spring fixed connection is in the one end of activity post, the other end fixed connection of activity post has the gear, one side meshing of gear is connected with the rack board, the upper end fixed mounting of rack board has a support panel, the upper end fixed mounting of support panel has the elevator, one side of support panel is provided with the slider, the one end of slider runs through sliding connection has the slide bar, the slide bar is fixed mounting at test box body inner chamber lateral wall, the apron is drawn the in-process, the apron draws the rope through fixed solid fixed ring, in the in-process of drawing, because the rope is connected with the winding of activity post, so drive the activity post and rotate, drive torsional spring and gear rotation simultaneously, drive rack board upward movement simultaneously at gear rotation, namely drive the support panel along the slide bar motion, drive the elevator upwards in reagent inslot chamber simultaneously, can be with the ejecting partly of reagent bottle, reached the effect that the operator conveniently took to the reagent bottle, easy operation can not cause the damage of reagent bottle at the in-process of taking to the problem in the background art has been solved.

In order to achieve the above purpose, the utility model provides a following technical scheme: the kit for the real-time fluorescence PCR detection of the embryonic cells by the one-step method comprises a test box body, wherein one end of the test box body is hinged with a cover plate, and the inner side of the test box body is provided with a lifting assembly;

the lifting assembly comprises a movable column and a torsional spring fixedly connected to one end of the movable column, the other end of the movable column is fixedly connected with a gear, and one side of the gear is meshed with a rack plate.

Preferably, the upper end fixed mounting of rack plate has support panel, and support panel's upper end fixed mounting has the elevator, and one side of support panel is provided with the slider, and sliding connection is run through to the one end of slider has the slide bar, and slide bar fixed mounting is at the internal cavity lateral wall of test box.

Preferably, the upper surface of the test box body is provided with a limit groove, one side of the limit groove is provided with a through groove arranged on the upper surface of the test box body, and one side of the through groove is provided with a reagent groove.

Preferably, the mounting groove has been seted up to the side surface of apron, fixedly connected with fixed column in the inner chamber of mounting groove, and the one end of fixed column runs through swing joint has the bracing piece, and the side surface both sides fixed mounting of apron has solid fixed ring, and solid fixed ring's one end through connection has the rope, the one end fixedly connected with handle of apron.

Preferably, the rope is connected with the movable column in a winding mode.

Preferably, the size of the reagent tank is matched with the size of the lifting block.

Preferably, the size of the fixing ring is matched with the size of the through groove.

Compared with the prior art, the beneficial effects of the utility model are as follows:

1. the utility model provides a pair of real-time fluorescence PCR detect reagent box of embryo cell one-step method, the mounting groove has been seted up to the side surface of apron, fixedly connected with fixed column in the inner chamber of mounting groove, the one end of fixed column runs through swing joint and has the bracing piece, the side surface both sides fixed mounting of apron has solid fixed ring, gu fixed ring's one end through connection has the rope, the one end fixedly connected with handle of apron, when needing to take out reagent bottle, the operator upwards draws the handle, the handle drives the apron and keeps away from the motion of test box body, when drawing the angle of conveniently taking reagent bottle, the motion of pulling bracing piece, the bracing piece is movable on the fixed column, put into the spacing groove with the bracing piece afterwards, can fix the apron, then carry out taking of reagent bottle.

2. The utility model provides a real-time fluorescence PCR detection kit of embryo cell one-step method, torsional spring fixed connection is in the one end of activity post, the other end fixed connection of activity post has the gear, the meshing of one side of gear is connected with the rack board, the upper end fixed mounting of rack board has a support panel, the upper end fixed mounting of support panel has the elevator, one side of support panel is provided with the slider, one end of slider runs through the sliding connection slide bar, the slide bar fixed mounting is at the test box body cavity lateral wall, the process of lid board being pulled, the lid board is pulled the rope through fixed solid fixed ring, in the process of pulling, because the rope is connected with the winding of activity post, so drive the activity post rotation, drive torsional spring and gear revolve simultaneously, drive the rack board upward movement while the gear revolve, namely drive the support panel along the slide bar motion, drive the elevator upwards in the reagent tank inner chamber simultaneously, can be with the ejecting partly of reagent bottle, reached the effect that the operator conveniently took to the reagent bottle, easy operation can not cause the damage of reagent bottle at the in-process of taking.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic view of the cover plate structure of the present invention;

fig. 3 is an enlarged view of a point a in fig. 2 according to the present invention;

fig. 4 is a plan sectional view of the lifting assembly of the present invention.

In the figure: 1. a test cartridge; 11. a limiting groove; 12. a through groove; 13. a reagent tank; 2. a cover plate; 21. mounting grooves; 22. fixing the column; 23. a support bar; 24. a fixing ring; 25. a rope; 26. a handle; 3. a lifting assembly; 31. a movable post; 32. a torsion spring; 33. a gear; 34. a rack plate; 35. a support panel; 36. a lifting block; 37. a slider; 38. a slide bar.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts all belong to the protection scope of the present invention.

Please refer to fig. 1, a kit for detecting embryo cells by one-step real-time fluorescence PCR comprises a testing box body 1, one end of the testing box body 1 is hinged with a cover plate 2, a limit groove 11 is arranged on the upper surface of the testing box body 1, one side of the limit groove 11 is provided with a through groove 12 arranged on the upper surface of the testing box body 1, when the cover plate 2 is closed with the testing box body 1, the fixing ring 24 is accommodated, one side of the through groove 12 is provided with a reagent groove 13, the size of the reagent groove 13 is matched with the size of a lifting block 36, and the inner side of the testing box body 1 is provided with a lifting component 3.

Referring to fig. 1, 2 and 4, the lifting assembly 3 includes a movable column 31 and a torsion spring 32 fixedly connected to one end of the movable column 31, when the reagent bottle upper cover plate 2 is taken out, the movable column 31 receives a reaction force of the torsion spring 32 to rewind the rope 25 onto the movable column 31, the other end of the movable column 31 is fixedly connected with a gear 33, one side of the gear 33 is engaged with a rack plate 34, the upper end of the rack plate 34 is fixedly provided with a support panel 35, the upper end of the support panel 35 is fixedly provided with a lifting block 36, the initial position of the lifting block 36 is flush with the bottom end of the reagent tank 13, one side of the support panel 35 is provided with a sliding block 37, one end of the sliding block 37 is connected with a sliding rod 38 in a penetrating and sliding manner, and the sliding rod 38 is fixedly installed on the side wall of the inner cavity of the test box body 1.

The in-process that apron 2 was drawn and was got, apron 2 draws rope 25 through fixed solid fixed ring 24 and gets, at the in-process of drawing and getting, because of rope 25 is connected with the winding of activity post 31, so drive activity post 31 and rotate, drive torsional spring 32 and gear 33 rotation simultaneously, drive rack plate 34 upward movement in gear 33 pivoted simultaneously, drive supporting panel 35 promptly and move along slide bar 38, drive elevator 36 upwards to slide in the inner chamber of reagent groove 13 simultaneously, can be with the ejecting partly of reagent bottle, the effect that the operator conveniently took to the reagent bottle has been reached, and the operation is thus simple, can not cause the damage of reagent bottle at the in-process of taking.

Referring to fig. 2-3, a mounting groove 21 is formed in a side surface of the cover plate 2, a fixing post 22 is fixedly connected to an inner cavity of the mounting groove 21, one end of the fixing post 22 is movably connected to a support rod 23 in a penetrating manner, fixing rings 24 are fixedly mounted on two sides of the side surface of the cover plate 2, one end of each fixing ring 24 is connected to a rope 25 in a penetrating manner, the size of each fixing ring 24 is matched with that of the corresponding penetrating groove 12, the rope 25 is connected to the corresponding movable post 31 in a winding manner, and one end of the cover plate 2 is fixedly connected to a handle 26.

When reagent bottle was taken out to needs, the operator upwards pulled handle 26, and handle 26 drives apron 2 and keeps away from test box body 1 motion, when pulling the angle of conveniently taking reagent bottle, pulling bracing piece 23 motion, bracing piece 23 is movable on fixed column 22, puts into spacing groove 11 with bracing piece 23 afterwards, can fix apron 2, then carries out taking of reagent bottle.

The working principle is as follows: when a reagent bottle needs to be taken out, an operator pulls the handle 26 upwards, the handle 26 drives the cover plate 2 to move away from the test box body 1, when the angle which is convenient for taking the reagent bottle is pulled, the support rod 23 is pulled to move on the fixed column 22, then the support rod 23 is placed into the limit groove 11, the cover plate 2 can be fixed, then the reagent bottle is taken, in the process of pulling the cover plate 2, the cover plate 2 pulls the rope 25 through the fixed fixing ring 24, in the pulling process, the rope 25 is wound and connected with the movable column 31, the movable column 31 is driven to rotate, the torsion spring 32 and the gear 33 are driven to rotate, the rack plate 34 is driven to move upwards while the gear 33 rotates, namely, the support panel 35 is driven to move, and the lifting block 36 is driven to slide upwards in the inner cavity of the reagent groove 13, and the reagent bottle can be pushed out partially, the reagent bottle can be taken out.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a real-time fluorescence PCR detect reagent box of embryo cell one-step method, includes test box body (1), its characterized in that: one end of the test box body (1) is hinged with a cover plate (2), and the inner side of the test box body (1) is provided with a lifting assembly (3);

lifting unit (3) are including activity post (31) and fixed connection at torsional spring (32) of activity post (31) one end, and the other end fixedly connected with gear (33) of activity post (31), and one side meshing of gear (33) is connected with rack plate (34).

2. The kit for detecting the real-time fluorescence PCR of the embryonic cell through the one-step method according to claim 1, which is characterized in that: the upper end fixed mounting of rack plate (34) has supporting panel (35), and the upper end fixed mounting of supporting panel (35) has elevator (36), and one side of supporting panel (35) is provided with slider (37), and sliding connection has slide bar (38) in the one end run-through sliding connection of slider (37), and slide bar (38) fixed mounting is at test box body (1) inner chamber lateral wall.

3. The kit for detecting the real-time fluorescence PCR of the embryonic cell through the one-step method according to claim 1, which is characterized in that: spacing groove (11) have been seted up to the upper surface of test box body (1), and one side of spacing groove (11) is provided with runs through groove (12) of seting up in test box body (1) upper surface, runs through one side of groove (12) and has seted up reagent groove (13).

4. The kit for detecting the real-time fluorescence PCR of the embryonic cell through the one-step method according to claim 3, which is characterized in that: mounting groove (21) have been seted up to the side surface of apron (2), fixedly connected with fixed column (22) in the inner chamber of mounting groove (21), and the one end of fixed column (22) runs through swing joint has bracing piece (23), and the side surface both sides fixed mounting of apron (2) has solid fixed ring (24), and the one end through connection of solid fixed ring (24) has rope (25), the one end fixedly connected with handle (26) of apron (2).

5. The kit for the one-step real-time fluorescence PCR detection of the embryonic cells according to claim 4, which is characterized in that: the rope (25) is connected with the movable column (31) in a winding way.

6. The kit for detecting the real-time fluorescence PCR of the embryonic cell through the one-step method according to claim 3, which is characterized in that: the size of the reagent groove (13) is matched with that of the lifting block (36).

7. The kit for detecting the real-time fluorescence PCR of the embryonic cell through the one-step method according to claim 4, which is characterized in that: the dimensions of the fixing ring (24) are matched to the dimensions of the through-groove (12).