CN216978969U

CN216978969U - Biological aerosol killing performance evaluation system

Info

Publication number: CN216978969U
Application number: CN202122196768.9U
Authority: CN
Inventors: 安太成; 王海余; 梁志梳; 李桂英
Original assignee: Guangdong University of Technology
Current assignee: Guangdong University of Technology
Priority date: 2021-09-10
Filing date: 2021-09-10
Publication date: 2022-07-15
Anticipated expiration: 2031-09-10

Abstract

The utility model provides a bioaerosol killing performance evaluation system, includes consecutive be used for atomizing microorganism sample solution into bioaerosol's bioaerosol generating device, be used for balancing bioaerosol's bioaerosol balancing unit and carry out the catalytic purification device that photocatalysis killed with balanced bioaerosol, catalytic purification device include with the purification barrel that bioaerosol balancing unit links to each other and with purify the sampling device that the barrel links to each other. The utility model provides an integrated evaluation device capable of performing bioaerosol simulation and photocatalytic killing.

Description

Biological aerosol killing performance evaluation system

Technical Field

The utility model relates to the technical field of bioaerosol killing, in particular to a system for evaluating the killing performance of bioaerosol.

Background

The traditional technologies for indoor air purification at present mainly include chemical reagent disinfection, activated carbon filtration, ozone disinfection, ultraviolet disinfection and the like, but all of them have certain limitations such as being unable to effectively remove bioaerosol and generating toxic by-products to cause secondary damage to people, so it is very necessary to vigorously develop environment-friendly air purification technologies. The photocatalytic technology is a green technology with important application prospect in the field of energy and environment, and the main principle of the photocatalytic technology is that the photocatalyst is activated under the light radiation, and forms active oxygen species to kill microorganisms and mineralize organic matters in a non-selective manner, so that secondary pollution is not generated. However, currently, the research on killing indoor air pollutants, particularly microbial aerosols, is very few, and the control effect is poor, so that the adoption of an advanced photocatalytic oxidation technology to realize the complete purification of indoor air can help to effectively reduce the potential risks of the microbial aerosols.

Chinese patent publication No. CN106769811A discloses an air microorganism experimental reaction device and its application, the device is used for studying the reaction change of microorganisms in air, and comprises a reaction chamber, an air gasification generator and an air sampler, an air inlet is arranged on one side wall of the reaction chamber and connected with the air gasification generator, and an air outlet is arranged on the other opposite side wall and connected with the air sampler; a fluorescent lamp and an ultraviolet lamp are installed at the top of the cabin, and a fan and a temperature and humidity sensor are arranged in the cabin; the reaction chamber has a volume of 150-500L and the inner wall is coated with a hydrophobic coating. However, the above scheme can only sample air, and the use process depends on environmental conditions, and a simulation experiment cannot be performed. In addition, the temperature in the cabin can be controlled only by changing the temperature of the external environment, and the limitation is large. In addition, the scheme only uses a fluorescent lamp and an ultraviolet lamp for disinfection, and does not have a related device for researching the photocatalytic killing effect.

SUMMERY OF THE UTILITY MODEL

The utility model aims to overcome the defects that an experimental device for evaluating the killing effect of photocatalysis on bioaerosol is complex to operate and an integrated device is not available in the prior art, and provides a system for evaluating the killing performance of bioaerosol. The utility model provides a device which can generate bioaerosol, carry out photocatalysis killing after the bioaerosol is balanced, and then evaluate the killing effect of the bioaerosol after killing by sampling and detecting.

The purpose of the utility model can be achieved by adopting the following technical scheme:

the utility model provides a biological aerosol kills performance evaluation system, includes consecutive biological aerosol generating device who is used for atomizing microorganism sample solution into biological aerosol, the biological aerosol balancing unit who is used for balancing biological aerosol and carry out the catalytic purification device that photocatalysis killed with biological aerosol after balancing, catalytic purification device includes the purification barrel who links to each other with biological aerosol balancing unit and the sampling device who links to each other with the purification barrel.

After the bioaerosol generated by the bioaerosol generator enters the balancing compartment for balancing, the bioaerosol is introduced into the purifying cylinder body in the catalytic purifying device for photocatalytic killing. The biological aerosol before and after killing is respectively detected, and the activity of the biological aerosol before and after killing is compared, so that the real-time photocatalysis killing effect evaluation can be realized.

Further, purify the barrel and be equipped with the removable filter that will purify the barrel and separate two parts, locate the photocatalyst on the filter, locate the ultraviolet lamp that is used for shining photocatalyst outside purifying the barrel, it is equipped with transparent irradiation window to lie in ultraviolet lamp department on the purifying the barrel. The filter plate can be foam metal such as foam nickel, foam copper and the like, and can also be a catalyst carrier such as porous ceramic and the like with high porosity and large specific surface area. The photocatalyst may be a transition metal oxide of iron, copper, zinc, or the like.

Furthermore, a fixing device for fixing the filter plate is arranged on the purifying cylinder and comprises a support ring fixedly connected to the inner annular wall of the purifying cylinder, an annular groove for supporting the filter plate is formed in the inner annular surface of the support ring, and the shape of the annular groove is matched with that of the filter plate. The filter is detachable, the change of the filter of being convenient for. The fixing device has good air tightness, so that aerosol can completely pass through the filter plate, and the real filtering effect of the filter plate is ensured.

Furthermore, a first adjusting device used for adjusting the distance between the ultraviolet lamp and the irradiation window is arranged on the ultraviolet lamp, and a second adjusting device used for adjusting the distance between the filter plate and the irradiation window is arranged between the fixing device and the purification cylinder. The first adjusting device and the second adjusting device are used for adjusting the distance between the filter plate and the ultraviolet lamp, and the ultraviolet light intensity irradiated to the filter plate is adjusted through adjusting the distance between the filter plate and the ultraviolet lamp, so that the catalytic efficiency of the catalyst is adjusted. The second adjusting device comprises a sealing ring arranged on the periphery of the support ring, a plurality of equidistant annular grooves are formed in the inner peripheral wall of the purifying cylinder, the sealing ring is arranged in the annular groove, the support ring can be arranged in the middle of the sealing ring due to the elasticity of the sealing ring, the support ring is propped against the sealing ring around the support ring, the support ring is made of an airtight material such as plastic, the distance between the filter plate and the irradiation window can be adjusted by fixing the sealing ring and the filter plate on different grooves, and meanwhile, gas can completely pass through the filter plate.

Furthermore, the filter plate is made of foam nickel, and the photocatalyst is iron oxyhydroxide. Foamed nickel is an excellent photocatalyst support. The preparation of the iron oxyhydroxide is mild in adjustment, good in stability and not easy to fall off, and can meet various environmental working conditions.

Furthermore, the purifying cylinder body is provided with a first air inlet connected with the biological aerosol balancing device and a first air outlet used for sampling, the first air inlet and the first air outlet are respectively arranged at two sides of the filter plate, and the first air inlet is provided with an air inlet control device used for controlling air inflow. The biological aerosol is generated by the biological aerosol generating device and then enters the purifying cylinder body through the first air inlet after being balanced by the biological aerosol balancing device. The filter separates the barrel with the fixing device of fixed filter, and first air inlet and first gas outlet are located respectively and are separated the both sides by the filter on purifying the barrel, and the gas that first gas outlet expert flows out must pass through the filter like this.

Furthermore, a branch structure is arranged on the first air outlet, an aerosol particle size spectrometer is arranged on one branch of the branch structure, and the other branch is connected with the sampling device. The particle size spectrometer can measure parameters such as the particle size of aerosol particles, the number concentration of the aerosol particles and the like, and the sampling device can be a liquid impact type air microorganism sampler.

Further, sampling device includes the inlet bend that links to each other with one end branch structure, the micropore nozzle that links to each other with the inlet bend, the sampling bottle that links to each other with the nozzle, the vacuum pump that links to each other with the sampling bottle, is equipped with sampling liquid in the sampling bottle, and sampling liquid can be phosphate buffer solution or normal saline etc.. The nozzle can convert the bioaerosol into a powerful jet flow, and the aerosol particles in the jet flow can be collected by the sampling liquid due to the adhesion of the liquid. The air inlet bent pipe is an arc-shaped right-angle bent pipe and is used for simulating the obstruction of the upper respiratory tract of a human to aerosol particles.

Further, the bioaerosol balancing device comprises a second air inlet and a second air outlet connected with the first air inlet, a third air outlet connected with the second air inlet is arranged on the purifying cylinder body, the third air outlet and the first air outlet are positioned on the same side, and a gas circulating device is arranged between the third air outlet and the second air inlet. The bioaerosol balancing device comprises a balancing compartment communicated with the bioaerosol generating device, a temperature control device for regulating and controlling the temperature of the aerosol in the balancing compartment, and a humidity control device for regulating and controlling the humidity of the aerosol in the balancing compartment, wherein the second air inlet and the second air outlet are arranged on the balancing compartment. The biological aerosol generated by the biological aerosol generating device is introduced into the balancing compartment, and after the biological aerosol is balanced for a certain time at a certain temperature and humidity, the biological aerosol enters the purifying cylinder body through the second air outlet. The biological aerosol enters the purification cylinder body, enters the air circulation system again through the third air outlet and then enters the balance chamber through the second air inlet after passing through the filter plate, and is circularly killed for many times, and the air circulation device is used for providing a power source for air circulation and controlling parameters such as the circulation flow, the circulation time and the like of air. The gas circulation device comprises a circulation pump and a controller.

Furthermore, the bioaerosol generating device comprises an air compressor, a pressure reducing valve, a flow controller air filter and a bioaerosol generator which are connected in sequence. The air filter is connected with the bioaerosol generator through an air quick connector. The air pumped by the air compressor can provide proper air pressure after passing through the pressure reducing valve. The air compressor and the flow control device are connected with the control device, and the start and stop of the air compressor and the pressure and the air flow required by the system are controlled according to set parameters, so that the concentration and the particle size distribution of the aerosol produced by the bioaerosol generator are changed. The air filter filters out dust, microorganisms and other pollutants in the air so as to prevent the biological aerosol in the balance compartment from being polluted.

Further, the bioaerosol balancing device comprises a balancing chamber, a temperature control system for adjusting the temperature in the balancing chamber and a humidity control system for adjusting the humidity in the balancing chamber, wherein the balancing chamber is respectively connected with the purifying cylinder and the bioaerosol generator.

The humidity control system comprises a humidity detector for detecting the humidity in the balance compartment, a reverse osmosis type drying tube for adjusting the humidity of the aerosol and a control device communicated with the humidity detector, wherein one end of the reverse osmosis type drying tube is connected with the biological aerosol generator, and the other end of the reverse osmosis type drying tube is connected with the balance compartment. When the reverse osmosis type drying tube is used, wet gas is introduced into the reverse osmosis membrane, then dry gas is introduced into a channel formed between the reverse osmosis membrane and the shell or a vacuum pump is used for pumping the channel to form a low-pressure environment, so that water vapor in the wet gas can diffuse out of the reverse osmosis membrane through the reverse osmosis membrane and is blown away by flowing air or pumped away by the vacuum pump.

The temperature control system comprises a temperature detector arranged in the balance chamber, a circulating water tank capable of providing circulating water with different temperatures, and a heat exchange structure connected with the circulating water tank and arranged on the outer wall of the balance chamber, wherein the temperature detector and the circulating water tank are connected with the control device. The heat exchange structure can encircle the circulating water pipe on the circulating water tank outer wall, also can be, balanced railway carriage or compartment is double-deck jacketed type structure, and the heat exchange structure is the skin of double-deck jacketed type structure, and thereby the circulating water enters into the intermediate layer of double-deck jacketed type structure, thereby carries out the temperature in the heat exchange control balanced railway carriage or compartment with balanced railway carriage or compartment, also can be other mechanisms that can realize the heat exchange, all do not influence the realization of this scheme. The control device is connected with the temperature detector and the circulating water tank, when the temperature detector monitors the temperature inside the balance compartment in real time and feeds the temperature back to the control device, the control device controls the operation of the circulating water tank according to set parameters, such as the temperature, the water flow speed, the start-stop time and the like of circulating water provided by the circulating water tank.

A method for evaluating the killing performance of bioaerosol comprises the following steps:

s1: after a microbial sample solution to be researched is cultured to a required concentration, injecting the microbial sample solution into a bioaerosol generating device, switching on a bioaerosol killing performance evaluation test device, and starting the bioaerosol generating device to generate bioaerosol;

S2: leading the bioaerosol produced by the bioaerosol generating device into the balance chamber, and closing the bioaerosol generating device after the bioaerosol generating device works for a period of time so that the bioaerosol is balanced in the balance chamber for a period of time;

s3: introducing the well-balanced bioaerosol into a catalytic purification device, turning on an ultraviolet lamp to irradiate a catalyst on a filter plate, and respectively sampling a first air inlet and a first air outlet on a purification cylinder of the catalytic purification device by using a sampling device;

s4: the collected samples are counted after being cultivated, and the sterilization efficiency is calculated through the following formula:

s5: the filter plate in the catalytic purification device is replaced by a filter plate without a catalyst, and the test is repeated to compare the sterilization efficiency data of two times.

Further, the balance time of the balance chamber is 30 minutes, the irradiation power of the ultraviolet lamp is 10W, the wavelength is 365nm, the irradiation time is 5 minutes, and the distance between the ultraviolet lamp and the filter plate is 5 centimeters.

Compared with the prior art, the utility model has the beneficial effects that:

(1) can evaluate the killing and purifying efficiency of the photocatalysis mode to the biological aerosol, and has simple and convenient operation.

(2) The filter can be changed with the kind of catalyst, can conveniently evaluate the effect of killing of different kinds of catalysts and filters.

(3) The activity of bioaerosols under different environments can be simulated through the bioaerosol balancing device, and a cyclic killing experiment can be carried out through the gas circulating device.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention.

FIG. 2 is a graph showing the change in aerosol concentration of bacteria before and after sterilization in accordance with the present invention.

FIG. 3 is a graph of the efficiency of the aerosol killing of bacteria according to the present invention.

FIG. 4 is a real-time equilibrium graph of biological activity of a bioaerosol of the present invention.

FIG. 5 is a graph of the real-time equilibrium biological relative humidity for a bioaerosol of the present invention.

FIG. 6 is a diagram illustrating the effect of the temperature control system of the present invention.

The graphic symbols are illustrated as follows:

1-bioaerosol generating device, 11-bioaerosol generator, 12-air compressor, 13-pressure reducing valve, 14-flow controller, 15-air filter, 2-bioaerosol balancing device, 21-balancing chamber, 211-second air outlet, 212-second air inlet, 22-reverse osmosis type drying tube, 3-catalytic purification device, 31-purification cylinder, 311-irradiation window, 312-first air inlet, 313-first air outlet, 314-third air outlet, 32-filter plate, 33-ultraviolet lamp, 34-gas circulation device and 35-aerosol particle size spectrometer.

Detailed Description

The present invention will be further described with reference to the following embodiments. Wherein the showings are for the purpose of illustration only and not for the purpose of limiting the same, the same is shown by way of illustration only and not in the form of limitation; to better illustrate the embodiments of the present invention, some parts of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted.

The same or similar reference numerals in the drawings of the embodiments of the present invention correspond to the same or similar components; in the description of the present invention, it should be understood that if there is an orientation or positional relationship indicated by the terms "upper", "lower", "left", "right", etc. based on the orientation or positional relationship shown in the drawings, it is only for convenience of describing the present invention and simplifying the description, but it is not intended to indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and therefore, the terms describing the positional relationship in the drawings are only used for illustrative purposes and are not to be construed as limiting the present patent, and the specific meaning of the terms may be understood by those skilled in the art according to specific circumstances.

Example 1

As shown in fig. 1, a bioaerosol killing performance evaluation system includes a bioaerosol generating device 1 for atomizing a microbial sample solution into bioaerosol, a bioaerosol balancing device 2 for balancing the bioaerosol, and a catalytic purification device 3 for performing photocatalytic killing on the balanced bioaerosol, which are connected in sequence, wherein the catalytic purification device 3 includes a purification cylinder 31 connected to the bioaerosol balancing device 2, and a sampling device connected to the purification cylinder 31.

The bioaerosol generating device 1 comprises a bioaerosol generator 11, the bioaerosol balancing device 2 comprises a balancing compartment 21, and bioaerosol generated by the bioaerosol generator 11 enters the balancing compartment 21 to be balanced and then is introduced into a purifying cylinder 31 in the catalytic purifying device 3 to be subjected to photocatalytic killing. The biological aerosol before and after killing is respectively detected, and the activity of the biological aerosol before and after killing is compared, so that the real-time photocatalysis killing effect evaluation can be realized.

As shown in fig. 1, a replaceable filter plate 32 for dividing the purification cylinder 31 into two parts, a photocatalyst disposed on the filter plate 32, and an ultraviolet lamp 33 disposed outside the purification cylinder 31 for irradiating the photocatalyst are disposed in the purification cylinder 31, and a transparent irradiation window 311 is disposed on the purification cylinder 31 at the position of the ultraviolet lamp 33.

As shown in fig. 1, the purification cylinder 31 is provided with a fixing device for fixing the filter plate 32, the fixing device includes a support ring fixedly connected to the inner annular wall of the purification cylinder 31, an annular groove for supporting the filter plate 32 is provided on the inner annular surface of the support ring, and the shape of the annular groove matches with the shape of the filter plate 32. The filter 32 is detachable, which facilitates the replacement of the filter 32. The fixing device has good air tightness, so that aerosol can completely pass through the filter plate 32 without overflowing from gaps and seams between the filter plate 32 and the purifying cylinder 31, and the real filtering effect of the filter plate 32 is ensured.

As shown in fig. 1, a first adjusting device for adjusting the distance between the ultraviolet lamp 33 and the irradiation window 311 is provided on the ultraviolet lamp 33, and a second adjusting device for adjusting the distance between the filter plate 32 and the irradiation window 311 is provided between the fixing device and the purification cylinder 31. The first adjusting device and the second adjusting device are used for adjusting the distance between the filter plate 32 and the ultraviolet lamp 33, and the intensity of ultraviolet light irradiated by the filter plate 32 is adjusted by adjusting the distance between the filter plate 32 and the ultraviolet lamp 33, so that the catalytic efficiency of the catalyst is adjusted. The second adjusting device comprises a sealing ring arranged on the periphery of the supporting ring, a plurality of equidistant annular grooves are formed in the inner peripheral wall of the purifying cylinder, the sealing ring is arranged in the annular groove, the supporting ring can be arranged in the middle of the sealing ring due to the elasticity of the sealing ring, the periphery of the supporting ring is propped against the sealing ring, the supporting ring is made of airtight materials such as plastics, the distance between the filtering plate 32 and the irradiation window 311 can be adjusted by fixing the sealing ring and the filtering plate 32 on different grooves, and meanwhile, all gas can pass through the filtering plate 32.

As shown in fig. 1, the purifying cylinder 31 is provided with a first air inlet 312 connected to the bioaerosol balancing device 2 and a first air outlet 313 for sampling, the first air inlet 312 and the first air outlet 313 are respectively disposed at two sides of the filter plate 32, and the first air inlet 312 is provided with an air inlet control device for controlling air inlet amount. The bioaerosol is generated by the bioaerosol generating device 1, and then enters the purifying cylinder 31 through the first air inlet 312 after being balanced by the bioaerosol balancing device 2. The filter plates 32 and the fixing means for fixing the filter plates 32 partition the purification cylinder 31, and the first gas inlet 312 and the first gas outlet 313 are respectively located at both sides of the purification cylinder 31 partitioned by the filter plates 32, so that the gas flowing through the first gas outlet 313 necessarily passes through the filter plates 32.

As shown in fig. 1, a branch structure is provided on the first air outlet 313, an aerosol particle size spectrometer 35 is provided on one branch of the branch structure, and the other branch is connected to a sampling device. The particle size spectrometer can measure parameters such as the particle size of aerosol particles and the number concentration of the aerosol particles, and the sampling device is a liquid impact type air microorganism sampler.

As shown in fig. 1, the sampling device includes an air inlet bent pipe connected with a branch structure at one end, a microporous nozzle connected with the air inlet bent pipe, a sampling bottle connected with the nozzle, and a vacuum pump connected with the sampling bottle, wherein a sampling solution is provided in the sampling bottle, and the sampling solution can be phosphate buffer solution or physiological saline solution. The nozzle can convert bioaerosol into powerful jet flow, and the sampling liquid collects aerosol particles in the jet flow due to the adhesiveness of the liquid. The air inlet bent pipe is an arc-shaped right-angle bent pipe and is used for simulating the obstruction of the upper respiratory tract of a human to aerosol particles.

As shown in fig. 1, the bioaerosol balancing device 2 includes a second air inlet 212 and a second air outlet 211 connected to the first air inlet 312, a third air outlet 314 connected to the second air inlet 212 is disposed on the purifying cylinder 31, the third air outlet 314 and the first air outlet 313 are located on the same side, and a gas circulation device 34 is disposed between the third air outlet 314 and the second air inlet 212. The bioaerosol balancing device 2 comprises a balancing compartment 21 communicated with the bioaerosol generating device 1, a temperature control device for regulating the temperature of the aerosol in the balancing compartment 21, and a humidity control device for regulating the humidity of the aerosol in the balancing compartment 21, wherein the humidity control device is a drying pipe arranged between the balancing compartment 21 and the bioaerosol generator 11, and the second air inlet 212 and the second air outlet 211 are arranged on the balancing compartment 21. The bioaerosol generated by the bioaerosol generating device 1 is introduced into the balancing compartment 21, and the bioaerosol enters the purifying cylinder 31 through the second air outlet 211 after being balanced for a certain time at a certain temperature and humidity. The bioaerosol enters the purifying cylinder 31, passes through the filter plate 32, and then enters the air circulation system again through the third air outlet 314, and then enters the balance chamber 21 through the second air inlet 212, so as to be circularly killed for multiple times, and the air circulation device 34 is used for providing a power source for air circulation and controlling parameters such as air circulation flow, circulation time and the like. The gas circulation device 34 includes a circulation pump and a controller.

As shown in fig. 1, the filter sheet 32 is a three-dimensional mesh material, and can carry a photocatalyst and allow a bioaerosol to pass therethrough. The filter plate 32 is foamed nickel and the photocatalyst is iron oxyhydroxide. Copper foam is an excellent photocatalyst support. The preparation of the iron oxyhydroxide is mild in adjustment, good in stability and not easy to fall off, and can meet various environmental working conditions.

As shown in fig. 1, the bioaerosol generating device 1 includes an air compressor 12, a pressure reducing valve 13, a flow controller 14, an air filter 15, and a bioaerosol generator 11 connected in sequence. Air filter 15 is connected to bioaerosol generator 11 by an air quick connector. The air pumped by the air compressor 12 can provide proper air pressure after passing through the pressure reducing valve 13. The air compressor 12 and the flow control device are connected with the control device, and the start and stop of the air compressor and the pressure and the air flow required by the system are controlled according to set parameters, so that the concentration and the particle size distribution of the aerosol produced by the bioaerosol generator 11 are changed. The air filter 15 filters contaminants such as dust, microorganisms, etc. from the air to prevent contamination of the bioaerosol in the balancing compartment 21.

As shown in fig. 1, the bioaerosol balancing device 2 includes a balancing compartment 21, a temperature control system for adjusting the temperature in the balancing compartment 21, and a humidity control system for adjusting the humidity in the balancing compartment 21, and the balancing compartment 21 is connected to the purification cylinder 31 and the bioaerosol generator 11, respectively.

The humidity control system comprises a humidity detector for detecting the humidity in the balance compartment 21, a reverse osmosis type drying pipe 22 for adjusting the humidity of the aerosol and a control device communicated with the humidity detector, wherein one end of the reverse osmosis type drying pipe 22 is connected with the biological aerosol generator 11, and the other end of the reverse osmosis type drying pipe is connected with the balance compartment 21. The reverse osmosis type drying tube 22 comprises a shell and a reverse osmosis membrane arranged in the shell, when the reverse osmosis type drying tube 22 is used, wet gas is introduced into the reverse osmosis membrane, dry gas is introduced into a channel formed between the reverse osmosis membrane and the shell or a vacuum pump is used for pumping the channel to form a low-pressure environment, so that water vapor in the wet gas diffuses out of the reverse osmosis membrane through the reverse osmosis membrane and is blown away by flowing air or pumped away by the vacuum pump.

The temperature control system comprises a temperature detector arranged in the balance compartment 21, a circulating water tank capable of providing circulating water with different temperatures, and a heat exchange structure connected with the circulating water tank and arranged on the outer wall of the balance compartment 21, wherein the temperature detector and the circulating water tank are connected with a control device. The balance compartment 21 is a double-layer jacket type structure, the heat exchange structure is an outer layer of the double-layer jacket type structure, circulating water enters an interlayer of the double-layer jacket type structure and exchanges heat with the balance compartment 21 to control the temperature in the balance compartment 21, the control device is connected with the temperature detector and the circulating water tank, when the temperature detector monitors the temperature in the balance compartment 21 in real time and feeds the temperature back to the control device, the control device controls the operation of the circulating water tank according to set parameters, such as the temperature, the water flow speed, the start-stop time and the like of the circulating water provided by the circulating water tank.

As shown in fig. 1, a magnetic fan is further disposed in the balance box 21, a power device of the magnetic fan is disposed outside the balance box 21, and an anti-adhesion coating is disposed on a surface of a blade of the magnetic fan. The magnetic fan reduces the sedimentation of the solutes in the bioaerosol by disturbing the gas inside the balancing compartment 21 so that the bioaerosol is more evenly distributed inside the balancing compartment 21. The non-stick coating can reduce solute particles from being adhered to fan blades of the magnetic fan, and avoid pollution caused by the adhered solute particles in the subsequent use process. Meanwhile, as the fan blades are driven by the power device arranged outside the balance compartment 21, the sealing performance of the balance compartment 21 is improved, and meanwhile, the electrified power device is not arranged inside the balance compartment 21, so that the attraction and adhesion of the biological aerosol to electrostatic force can be reduced. The fan blades of the magnetic fan are arranged in the center of the bottom of the balance compartment 21, the disturbance state and the retention time of the airflow can be effectively changed, and the anti-adhesion coating is made of polytetrafluoroethylene.

As shown in fig. 1, an air pressure detector is disposed in the balancing compartment 21 for detecting air pressure inside the balancing compartment 21, and a flow divider for controlling flow rate of the bio-aerosol entering the balancing compartment 21 is disposed between the balancing compartment 21 and the bio-aerosol generator 11. The flow of the bioaerosol entering the balance compartment 21 is controlled through the flow divider, so that the air pressure in the balance compartment 21 is controlled, redundant bioaerosol is discharged by the flow divider and does not enter the balance compartment 21, and the discharged bioaerosol is discharged after harmless treatment.

As shown in fig. 1, the bioaerosol generator 11 is a liquid microbial aerosol generator 11.

As shown in fig. 1, the inner wall of the balance box 21 is provided with an anti-stick layer. The anti-adhesion layer can reduce the adhesion of microorganisms on the inner wall of the balance box 21 and avoid the corrosion of the microorganisms on the inner wall of the balance box 21. The anti-sticking layer is a polytetrafluoroethylene layer.

As shown in fig. 1, a cleaning system for cleaning residual microorganisms is also provided in the balancing compartment 21. The cleaning system includes a uv lamp 33 which is sterilizable.

Example 2

This example is similar to example 1 except that the filter plate 32 is copper foam and the photocatalyst is zinc oxide.

Example 3

s1: culturing a microbial sample solution to be researched to a required concentration, injecting the microbial sample solution into a bioaerosol generating device 1, switching on a bioaerosol killing performance evaluation test device, and starting the bioaerosol generating device 1 to generate bioaerosol;

s2: leading the bioaerosol produced by the bioaerosol generating device 1 into the balancing compartment 21, and closing the bioaerosol generating device 1 after the bioaerosol generating device 1 works for a period of time so as to balance the bioaerosol in the balancing compartment 21 for a period of time;

S3: introducing the balanced biological aerosol into the catalytic purification device 3, turning on the ultraviolet lamp 33 to irradiate the catalyst on the filter plate 32, and sampling the first air inlet 312 and the first air outlet 313 on the purification cylinder 31 of the catalytic purification device 3 by using a sampling device respectively;

s5: the filter plate 32 in the catalytic purification device 3 is replaced by the filter plate 32 without the catalyst, and the test is repeated to compare the sterilization efficiency data of two times.

As shown in FIG. 1, the balancing time of the balancing compartment 21 is 30 minutes, the irradiating power of the ultraviolet lamp 33 is 10W, the wavelength is 365nm, the irradiating time is 5 minutes, and the distance between the ultraviolet lamp 33 and the filter plate 32 is 5 cm.

As shown in fig. 2 and fig. 3, compared with the blank control group, the bacterial aerosol concentration of the nickel foam without any modification treatment is not changed significantly after being irradiated by the ultraviolet lamp 33, and the sterilization efficiency is only 32.5%; and the foam nickel of FeOOH nano-sheets grows in situ, so that the concentration of bacterial aerosol is reduced more obviously, that is, more organisms are killed, and the sterilization efficiency can reach about 99 percent. Therefore, the photocatalytic sterilization purification device can be fully proved to completely meet the research requirements, and the experimental method is completely scientific and reasonable and is very suitable for photocatalytic air sterilization research.

Example 4

A method of balancing bioaerosol generation and homeostasis, comprising the steps of:

s1: selecting Escherichia coli as sample strain, properly amplifying to obtain OD_600nmTaking a proper amount of the generating solution which is 1 +/-0.1 in a clean workbench, transferring the generating solution into a liquid containing bottle of the sterilized biological aerosol generator 111, sealing, and transferring for waiting for the next gas path access operation.

S2: the bioaerosol generator 11 filled with the generation liquid is horizontally fixed on a base, the relative height between the internal device of the generator and the liquid level is adjusted, then the generator is connected with a gas circuit and the gas tightness and the pipeline flow are checked, and the aerosol generation procedure can be carried out after the checking is correct.

S3: the bioaerosol generator 11 is closed after working for 20 minutes, bioaerosol enters the balancing compartment 21 for dynamic balancing, the balancing time is 1 hour, and the collected sample is subjected to 10 times of physiological saline⁰、10^-1、 10^-2、10^-3、10^-4And 10^-5After 100 microliters of the agar plates are coated, placing the agar plates in a constant-temperature incubator at 37 ℃ for incubation for 24 hours, initially counting, then incubating for 24 hours to 48 hours for supplementary counting, and obtaining the concentration of bacteria in the collected aerosol sample according to a calculation formula:

S4: at the same time of step S3, the time-dependent changes of the temperature and the relative humidity in the balancing compartment 21 are recorded in real time to evaluate the balancing efficiency of the balancing compartment 21 for the generated bioaerosol.

S5: and repeating the steps S1-S4, configuring the generating solution with the same concentration to perform repeated experiments, wherein the working time of the bioaerosol generator 11 in each experiment is the same, but the time for the bioaerosol in each experiment to enter the balancing compartment 21 for dynamic balancing is increased by 1 hour until the dynamic balancing time of the bioaerosol in the balancing compartment 21 is 18 hours. Namely, the dynamic balance time of the first experiment is 1 hour, the dynamic balance time of the second experiment is 2 hours, the dynamic balance time of the third experiment is 3 hours, and the experiment can be finished after the eighteenth experiment is finished until the dynamic balance time of the eighteenth experiment is 18 hours.

As shown in fig. 4, fig. 4 is a real-time balance diagram of biological activity of bioaerosol in the present invention, and it can be seen from fig. 4 that the concentration of bacterial aerosol in the balance compartment 21 does not change significantly within 1 hour, which indicates that the activity of bacteria does not decrease significantly, and does not change significantly within 0-18 hours of the long-scale time period, which indicates that the dynamic balance effect of the balance compartment 21 on bioaerosol is very good, and the air tightness can completely meet the experimental requirements, and the gradual decrease of the bacterial concentration at this time may be attributed to the shortage of nutrients of bacteria itself and other pressure injury not given by the environmental change in the balance compartment 21, so that it can be fully demonstrated that the bioaerosol generating and balancing apparatus designed by us has better stability and applicability.

As shown in fig. 5, fig. 5 is a real-time equilibrium diagram of biological relative humidity of bioaerosol in the present invention, and it can be seen from fig. 5 that only slight fluctuation of humidity in the equilibrium chamber 21 does not change significantly within 18 hours of continuous equilibrium, which indicates that the equilibrium chamber 21 has very good dynamic equilibrium effect on bioaerosol, and the air tightness can completely satisfy the experimental requirements.

As shown in fig. 6, fig. 6 is a diagram illustrating the effect of the temperature control system according to the present invention, and it can be seen from fig. 6 that the temperature inside the balancing compartment 21 can be rapidly and accurately controlled in a short time, which means that the temperature control system can rapidly and accurately control the internal temperature of the balancing compartment 21, and completely meet the research requirements related to bioaerosols at different temperatures.

It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. The system for evaluating the killing performance of the bioaerosol is characterized by comprising a bioaerosol generating device (1) for atomizing a microorganism sample solution into the bioaerosol, a bioaerosol balancing device (2) for balancing the bioaerosol and a catalytic purification device (3) for carrying out photocatalytic killing on the balanced bioaerosol, wherein the catalytic purification device (3) comprises a purification cylinder body (31) connected with the bioaerosol balancing device (2) and a sampling device (36) connected with the purification cylinder body (31).

2. The bioaerosol killing performance evaluation system according to claim 1, wherein the purification cylinder (31) is provided therein with a replaceable filter plate (32) for dividing the purification cylinder (31) into two parts, a photocatalyst disposed on the filter plate (32), and an ultraviolet lamp (33) disposed outside the purification cylinder (31) for irradiating the photocatalyst, and the purification cylinder (31) is provided thereon with a transparent irradiation window (311) at the position of the ultraviolet lamp (33).

3. The bioaerosol killing performance evaluation system according to claim 2, wherein the purifying cylinder (31) is provided with a fixing device for fixing the filter plate (32), the fixing device comprises a support ring fixedly connected to the inner annular wall of the purifying cylinder (31), the inner annular surface of the support ring is provided with an annular groove for supporting the filter plate (32), and the shape of the annular groove matches with the shape of the filter plate (32).

4. The bioaerosol killing performance evaluation system according to claim 3, wherein the catalytic purification device (3) further comprises a first adjusting means for adjusting a distance between the ultraviolet lamp (33) and the irradiation window (311), and a second adjusting means provided between the fixing means and the purification cylinder (31) for adjusting a distance between the filter plate (32) and the irradiation window (311).

5. The bioaerosol killing performance evaluation system according to claim 2, wherein the purifying cylinder (31) is provided with a first air inlet (312) connected to the bioaerosol balancing device and a first air outlet (313) for sampling, the first air inlet (312) and the first air outlet (313) are respectively arranged at two sides of the filter plate (32), and the first air inlet (312) is provided with an air inlet control device for controlling air inlet amount.

6. The bioaerosol killing performance evaluation system according to claim 5, wherein a branch structure is arranged on the first air outlet (313), an aerosol particle size spectrometer (35) is arranged on one branch of the branch structure, and the other branch is connected with the sampling device (36).

7. The bioaerosol killing performance evaluation system of claim 6, wherein the sampling device (36) comprises an air inlet elbow connected to the branch structure at one end, a micropore nozzle connected to the air inlet elbow, a sampling bottle connected to the nozzle, and a vacuum pump (361) connected to the sampling bottle, wherein the sampling bottle is filled with a sampling liquid.

8. The bioaerosol killing performance evaluation system of any one of claims 5 to 7, wherein the bioaerosol balancing means (2) comprises a second gas inlet (212) and a second gas outlet (211) connected to the first gas inlet (312), a third gas outlet (314) connected to the second gas inlet (212) is provided on the purification cartridge (31), the third gas outlet (314) is located on the same side as the first gas outlet (313), and a gas circulation means (34) is provided between the third gas outlet (314) and the second gas inlet (212).

9. The bioaerosol killing performance evaluation system according to claim 1, wherein the bioaerosol generating device (1) comprises an air compressor (12), a pressure reducing valve (13), a flow controller (14), an air filter (15), and a bioaerosol generator (11) which are connected in sequence.

10. The bioaerosol killing performance evaluation system according to claim 9, wherein the bioaerosol balancing device (2) comprises a balancing compartment (21), a temperature control system for adjusting the temperature in the balancing compartment (21), and a humidity control system for adjusting the humidity in the balancing compartment (21), the balancing compartment (21) being connected to the purification cartridge (31) and the bioaerosol generator (11), respectively.