CN216738391U

CN216738391U - Bioreactor for preparing monoclonal antibody

Info

Publication number: CN216738391U
Application number: CN202123326526.3U
Authority: CN
Inventors: 曹亮
Original assignee: Nanjing Huading Biotechnology Co ltd
Current assignee: Nanjing Huading Biotechnology Co ltd
Priority date: 2021-12-27
Filing date: 2021-12-27
Publication date: 2022-06-14
Anticipated expiration: 2031-12-27

Abstract

The utility model discloses a bioreactor for preparing monoclonal antibody, which comprises a fixed base, a reactor body and a console, wherein the reactor body and the console are arranged on the fixed base; the bottom of one side of the gas purification box is provided with a fan, the outer side of the fan is communicated with the reactor body, and the inner side of the fan is connected with a gas pipe; the bottom end of the filter liquid chamber is also provided with a spray pipe facing the air purifying chamber; a driving motor is fixed on the side face of the fixed base, an output shaft of the driving motor is connected with a bidirectional screw rod, and a sliding block is connected to the surface of the bidirectional screw rod in a threaded manner. The bioreactor for preparing the monoclonal antibody can filter gas discharged in the biological reaction process, improves the use environmental protection performance, is convenient to move, enlarges the use range, reduces the manual carrying cost, and can effectively prevent the device from being damaged in the carrying process.

Description

Bioreactor for preparing monoclonal antibody

Technical Field

The utility model belongs to the technical field of monoclonal antibody preparation, and particularly relates to a bioreactor for preparing a monoclonal antibody.

Background

The monoclonal antibody is produced by artificially prepared hybridoma cells, the hybridoma cells are formed by fusing B lymphocytes activated by antigens and myeloma cells, and the monoclonal antibody has the advantages of high purity, strong specificity, high sensitivity, less cross reaction, low preparation cost and the like.

When the monoclonal antibody is prepared, a biological reaction is usually required to be carried out in a bioreactor, sometimes harmful substances are generated in the reaction process, and are not specially treated at present, and the harmful substances are discharged along with gas generated by the reaction, so that the surrounding environment and workers are affected, and the preparation method is not environment-friendly; in addition, the bioreactor required by some reactions has large volume and heavy weight, and needs manual carrying during moving, so that on one hand, a large amount of manpower is consumed, the use is inconvenient, and on the other hand, collision and the like are easy to occur in the carrying process, so that the internal elements of the bioreactor are damaged, and the service life is influenced.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a bioreactor for preparing monoclonal antibody, which can filter gas discharged in the biological reaction process and improve the use environmental protection performance.

Another object of the present invention is to provide a bioreactor for monoclonal antibody preparation, which is convenient to move, has an expanded application range, reduces the manual handling cost, and can effectively prevent the damage to the device during the handling process.

In order to achieve the above purpose, the solution of the utility model is:

a bioreactor for preparing monoclonal antibody comprises a fixed base, a reactor body and a control console, wherein the reactor body and the control console are arranged on the fixed base;

the reactor comprises a reactor body, wherein a gas purifying mechanism is fixed at the front end of the reactor body, the gas purifying mechanism comprises a gas purifying box, the gas purifying box is fixedly connected to the side surface of the reactor body, a fan is arranged at the bottom of one side of the gas purifying box, the outer side of the fan is communicated with the reactor body, and the inner side of the fan is connected with a gas delivery pipe; the air purifying box is internally provided with an air purifying chamber and a filtrate chamber, the air purifying chamber is positioned below the filtrate chamber, the bottom end of the air purifying chamber is communicated to the filtrate chamber through a return pipe, and the bottom end of the filtrate chamber is also provided with a spray pipe facing the air purifying chamber;

the side of unable adjustment base is provided with moving mechanism, and moving mechanism is including being fixed in the driving motor of unable adjustment base side, and driving motor's output shaft has two-way screw rod, and two-way screw rod's surperficial threaded connection has the sliding block, and the bottom swing joint of sliding block has the connecting rod, and the bottom swing joint of connecting rod has the supporting leg, and unable adjustment base and swing joint have the removal wheel are worn out to the bottom of supporting leg.

The one end fixedly connected with backwash pump of above-mentioned back flow, the top in filtration filtrate chamber is provided with the liquid feeding valve, and the shower extends to the inside in air-purifying chamber to can spray the filtration to the gas that discharges, and realize the recycling of filtrate, avoid the waste of resource.

The top side fixedly connected with of above-mentioned air-purifying chamber adsorbs the cotton, adsorbs cotton top fixedly connected with filter screen, and the filter screen setting is at the top of air-purifying box to adsorb the filtration to the harmful substance in the gas, improve the feature of environmental protection.

The side swing joint of above-mentioned supporting leg has the limiting plate, and the right side top of limiting plate is provided with the tight mechanism in top, the top and the two-way screw rod fixed connection of the tight mechanism in top to the convenience is restricted the supporting leg, avoids contracting of supporting leg.

The tight mechanism in above-mentioned top includes the action wheel, and action wheel fixed connection is connected with from the driving wheel in the middle part of two-way screw rod, and the side meshing of action wheel is connected with from the driving wheel, follows the bottom fixedly connected with threaded rod of driving wheel, and the convenience is controlled the tight in top of restriction board.

The surface threaded connection of above-mentioned threaded rod has the movable block, and the bottom fixedly connected with of movable block pushes up tight spring, pushes up the bottom fixedly connected with kicking block of tight spring, through the cooperation of movable block and the tight spring in top, pushes up tightly the limiting plate through the kicking block, and the convenience is to the control of supporting leg.

After adopting the scheme, compared with the prior art, the utility model has the beneficial effects that:

(1) the utility model provides a bioreactor for preparing monoclonal antibody, which is characterized in that a fan, a gas pipe, a gas purifying chamber, a filter liquor chamber, a spray pipe, adsorption cotton and a filter screen are matched with each other, gas is introduced into the gas purifying chamber through the fan, and is subjected to spray filtration of filter liquor and adsorption filtration of the adsorption cotton and the filter screen, so that the gas can be discharged, the problem that the existing bioreactor for preparing monoclonal antibody is lack of filtration of discharged gas is solved, the pollution to the environment is avoided, and the use of the device is facilitated;

(2) the utility model provides a bioreactor for preparing monoclonal antibodies, which is characterized in that a driving motor, a bidirectional screw rod, a sliding block, a connecting rod, supporting legs and a moving wheel are matched with each other, the driving motor drives the bidirectional screw rod to rotate, and the sliding block drives the connecting rod to move, so that the supporting legs can be ejected out, the moving wheel extends out, the problem that the existing bioreactor for preparing the monoclonal antibodies is inconvenient to move when in use is solved, the movement of the device is facilitated, and the practicability of the bioreactor is improved.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic sectional view of the air purifying mechanism according to the present invention;

FIG. 3 is a schematic sectional view of the moving mechanism of the present invention;

fig. 4 is a schematic sectional view of the tightening mechanism according to the present invention.

Reference numerals:

1. a reactor body; 11. a stirring device;

2. a fixed base; 3. a console;

4. a gas purification mechanism; 41. a gas purification box; 42. a fan; 43. a gas delivery pipe;

44. a gas purifying chamber; 45. a return pipe; 46. a filtrate chamber; 47. a shower pipe;

48. adsorbing cotton; 49. a filter screen;

5. a moving mechanism; 51. a drive motor; 52. a bidirectional screw; 53. a slider;

54. a connecting rod; 55. supporting legs; 56. a moving wheel; 57. a limiting plate;

58. a jacking mechanism; 581. a driving wheel; 582. a driven wheel;

583. a threaded rod; 584. a moving block; 585. the spring is tightly propped; 586. and (7) a top block.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative and intended to explain the present invention and should not be construed as limiting the present invention.

As shown in fig. 1, the utility model provides a bioreactor for preparing monoclonal antibodies, which comprises a reactor body 1, wherein a stirring device 11 is arranged at the top of the reactor body 1, a fixed base 2 is fixedly connected at the bottom end of the reactor body 1, a control console 3 is fixedly connected at the top end of the fixed base 2 and adjacent to the reactor body 1, a gas purifying mechanism 4 is fixedly connected at the front end of the reactor body 1, and a moving mechanism 5 is arranged on the side surface of the fixed base 2; with reference to fig. 2, the air purifying mechanism 4 includes an air purifying tank 41, the air purifying tank 41 is fixedly connected to the side of the reactor body 1, a left bottom fixedly connected with fan 42 of the air purifying tank 41, a side fixedly connected with air pipe 43 of the fan 42, one end of the air pipe 43 is provided with an air purifying chamber 44, a bottom side of the air purifying chamber 44 is provided with a return pipe 45, one end of the return pipe 45 is provided with a filtrate chamber 46, a bottom end of the filtrate chamber 46 is provided with a spray pipe 47, one end fixedly connected with reflux pump of the return pipe 45, a top of the filtrate chamber 46 is provided with a liquid adding valve, the spray pipe 47 extends to the inside of the air purifying chamber 44, a top side fixedly connected with adsorption cotton 48 of the air purifying chamber 44, a top fixedly connected with a filter screen 49 of the adsorption cotton 48, and the filter screen 49 is arranged at the top of the air purifying tank 41.

In the embodiment, the reactor body 1 can be moved by the moving mechanism 5, so that the device is convenient to use; can control reactor body 1's work through control cabinet 3, and accessible agitating unit 11 stirs the material in the reactor body 1, still can be through the gas of fan 42 in with reactor body 1 during the use, introduce air-purifying chamber 44 through gas-supply pipe 43, the filtrate blowout in rethread shower 47 will filtrate chamber 46, thereby spray and filter gas, and through the setting of back flow 45 and backwash pump, make things convenient for during the filtrate refluxes filtrate chamber 46, the cyclic utilization of filtrate has been realized, gas in the air-purifying chamber 44 will be discharged after the adsorption and filtration of adsorption cotton 48 and filter screen 49 again, thereby the pollution of environment has been avoided.

As a preferred embodiment of the present invention, as shown in fig. 3, the moving mechanism 5 includes a driving motor 51, the driving motor 51 is fixedly connected to the left side of the fixed base 2, an output shaft of the driving motor 51 is fixedly connected with a bidirectional screw 52, a side surface of the bidirectional screw 52 is in threaded connection with a sliding block 53, a bottom end of the sliding block 53 is movably connected with a connecting rod 54, a bottom end of the connecting rod 54 is movably connected with a supporting leg 55, a bottom end of the supporting leg 55 is movably connected with a moving wheel 56, a side surface of the supporting leg 55 is movably connected with a limiting plate 57, a top portion of the right side of the limiting plate 57 is provided with a tightening mechanism 58, and a top portion of the tightening mechanism 58 is fixedly connected with the bidirectional screw 52.

In this embodiment, when reactor body 1 needs to move, drive two-way screw 52 through driving motor 51 and rotate, and make sliding block 53 remove in two-way screw 52's side, thereby release supporting leg 55 through connecting rod 54, thereby make removal wheel 56 stretch out unable adjustment base 2, thereby conveniently remove reactor body 1, will drive the removal of limiting plate 57 through the spout of side when supporting leg 55 stretches out, and can restrict limiting plate 57 through tight mechanism 58 in top, thereby avoid the withdrawal of supporting leg 55, the removal of device has been made things convenient for.

As a preferred embodiment of the present invention, as shown in fig. 4, the tightening mechanism 58 includes a driving wheel 581, the driving wheel 581 is fixedly connected to the middle portion of the bidirectional screw 52, a driven wheel 582 is engaged and connected to the side surface of the driving wheel 581, a threaded rod 583 is fixedly connected to the bottom end of the driven wheel 582, a moving block 584 is threadedly connected to the side surface of the threaded rod 583, a tightening spring 585 is fixedly connected to the bottom end of the moving block 584, and a tightening block 586 is fixedly connected to the bottom end of the tightening spring 585.

In this embodiment, when the bidirectional screw 52 rotates, the driving wheel 581 is driven to rotate, so that the driven wheel 582 rotates, the threaded rod 583 is driven to rotate, the moving block 584 moves downwards through the thread fit with the moving block 584, the jacking spring 585 is jacked, the jacking block 586 is jacked, the limiting plate 57 is limited by the jacking block 586, the supporting legs 55 are prevented from retracting, and the device is convenient to move.

The working principle of the utility model is as follows: drive two-way screw rod 52 through driving motor 51 and rotate, and make sliding block 53 remove, thereby release supporting leg 55 through connecting rod 54, make to remove wheel 56 and stretch out unable adjustment base 2, thereby the convenience removes reactor body 1, the use of device has been made things convenient for, can control reactor body 1's work through control cabinet 3, and accessible agitating unit 11 stirs the material in the reactor body 1, still can pass through the gas in fan 42 with reactor body 1 during the use, introduce air-purifying chamber 44 through gas-supply pipe 43, the filtrating of rethread shower 47 in with filtrate chamber 46 spouts, thereby spray the filtration to gas, discharge after the adsorption filtration of adsorbing cotton 48 and filter screen 49 again, thereby the pollution of environment has been avoided.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations and positional relationships based on those shown in the drawings, and are used only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the utility model. In this specification, the schematic representations of the terms used above should not be understood to necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples described in this specification can be combined and combined by those skilled in the art.

Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.

Claims

1. A bioreactor for monoclonal antibody production, comprising: comprises a fixed base (2), a reactor body (1) and a control console (3) which are arranged on the fixed base (2), wherein the top of the reactor body (1) is provided with a stirring device (11);

the reactor is characterized in that a gas purifying mechanism (4) is fixed at the front end of the reactor body (1), the gas purifying mechanism (4) comprises a gas purifying box (41), the gas purifying box (41) is fixedly connected to the side surface of the reactor body (1), a fan (42) is arranged at the bottom of one side of the gas purifying box (41), the outer side of the fan (42) is communicated with the reactor body (1), and the inner side of the fan (42) is connected with a gas conveying pipe (43); an air purifying chamber (44) and a filtrate chamber (46) are arranged in the air purifying tank (41), the air purifying chamber (44) is positioned below the filtrate chamber (46), the bottom end of the air purifying chamber (44) is communicated to the filtrate chamber (46) through a return pipe (45), and a spray pipe (47) facing the air purifying chamber (44) is further arranged at the bottom end of the filtrate chamber (46);

the side of unable adjustment base (2) is provided with moving mechanism (5), moving mechanism (5) are including being fixed in driving motor (51) of unable adjustment base (2) side, the output shaft of driving motor (51) has two-way screw rod (52), the surperficial threaded connection of two-way screw rod (52) has sliding block (53), and the bottom swing joint of sliding block (53) has connecting rod (54), the bottom swing joint of connecting rod (54) has supporting leg (55), unable adjustment base (2) and swing joint have removal wheel (56) are worn out to the bottom of supporting leg (55).

2. A bioreactor for monoclonal antibody production according to claim 1, wherein: one end of the return pipe (45) is fixedly connected with a return pump, the top of the filtrate chamber (46) is provided with a liquid adding valve, and the spray pipe (47) extends into the air purifying chamber (44).

3. A bioreactor for monoclonal antibody production according to claim 1, wherein: the top side fixedly connected with of air-purifying chamber (44) adsorbs cotton (48), adsorbs the top fixedly connected with filter screen (49) of cotton (48), and filter screen (49) set up the top at air-purifying box (41).

4. A bioreactor for monoclonal antibody production according to claim 1, wherein: the side face of the supporting leg (55) is movably connected with a limiting plate (57), the top of the right side of the limiting plate (57) is provided with a jacking mechanism (58), and the top of the jacking mechanism (58) is fixedly connected with the bidirectional screw (52).

5. A bioreactor for monoclonal antibody production according to claim 4, wherein: the jacking mechanism (58) comprises a driving wheel (581), the driving wheel (581) is fixedly connected to the middle of the bidirectional screw (52), the side face of the driving wheel (581) is meshed with a driven wheel (582), and a threaded rod (583) is fixedly connected to the bottom end of the driven wheel (582).

6. A bioreactor for monoclonal antibody production according to claim 5, wherein: the surface threaded connection of threaded rod (583) has movable block (584), and the bottom fixedly connected with of movable block (584) pushes up tight spring (585), pushes up the bottom fixedly connected with kicking block (586) of tight spring (585).