CN216665872U - Fluid conveying equipment and cell perfusion culture device - Google Patents

Abstract

The embodiment of the specification provides a fluid conveying device and a cell perfusion culture device. The fluid delivery apparatus includes: the extruding mechanism comprises a roller body and a helical blade arranged on the roller body; the driving mechanism is used for driving the roller body to rotate along the axis of the roller body; a plurality of conveying pipelines for the circulation of fluid, wherein the conveying pipelines comprise extrusion sections for being extruded by the helical blades; the extrusion sections of the conveying pipelines extend along the axial direction of the roller body, and the extrusion sections of the conveying pipelines are arranged at intervals along the axial direction of the roller body; wherein the helical blade is configured to push against the extrusion section as the roller body rotates to cause fluid flow in each of the transfer lines.

Description

Fluid conveying equipment and cell perfusion culture device

Technical Field

The present specification relates to the field of fluid delivery, and in particular, to a fluid delivery apparatus and a cell perfusion culture device.

Background

The liquid conveying equipment is widely applied to the industries of experimental research, biological pharmacy, analytical instruments, medical treatment, food, chemical engineering and the like. In perfusion culture, the fluid transfer device may be used to transfer various culture solutions, to lead out waste solutions, and the like. At present, in a cell perfusion culture system, fluid can be conveyed by matching a peristaltic pump and a hose, and the peristaltic pump can alternately extrude the hose through a plurality of rollers to convey the fluid in the hose.

SUMMERY OF THE UTILITY MODEL

Embodiments of the present description provide a fluid delivery apparatus, which includes: the extruding mechanism comprises a roller body and a helical blade arranged on the roller body; the driving mechanism is used for driving the roller body to rotate along the axis of the roller body; a plurality of conveying pipelines for the circulation of fluid, wherein the conveying pipelines comprise extrusion sections for being extruded by the helical blades; the extrusion sections of the conveying pipelines extend along the axis direction of the roller body, and are arranged at intervals along the axis direction of the roller body; wherein the helical blade is configured to push against the extrusion section as the roller body rotates to cause fluid flow in each of the delivery lines.

In some embodiments, the inlet ends of the plurality of conveying pipelines are respectively communicated with different fluid storage mechanisms, and the outlet ends of the plurality of conveying pipelines are respectively connected with different target conveying mechanisms.

In some embodiments, the helical blades are variable pitch helical blades, and the pitch of the extruded sections of at least two of the plurality of conveying lines is different.

In some embodiments, the helical blade is a constant pitch helical blade.

In some embodiments, the fluid transfer apparatus further comprises a pressure-bearing plane, and the extruded sections of the plurality of transfer lines are located between the pressure-bearing plane and the extrusion mechanism.

In some embodiments, the surface of the helical blade in contact with the extruded section and/or the exterior of the extruded section is provided with an anti-wear layer.

In some embodiments, the pitch of the helical blade is 1mm to 50 mm; the external spiral radius of the spiral blade is 1 mm-100 mm.

In some embodiments, the roller body is cylindrical, and the ratio of the outer spiral radius of the spiral blade to the radius of the roller body is 10: 1-10: 9.

In some embodiments, the helical blade has a thickness of 0.2mm to 20 mm.

An embodiment of the present disclosure further provides a perfusion culture apparatus for cells, which includes an incubator, wherein at least a part of the fluid transfer device according to any of the above-mentioned aspects is disposed in the incubator.

Drawings

The present description will be further explained by way of exemplary embodiments, which will be described in detail by way of the accompanying drawings. These embodiments are not intended to be limiting, and in these embodiments like numerals are used to indicate like structures, wherein:

FIG. 1 is a schematic block diagram of a fluid delivery apparatus according to some embodiments herein;

fig. 2 is a schematic diagram of a cell perfusion culture system according to some embodiments of the present disclosure.

Description of reference numerals: 1000. a cell perfusion culture device; 100. a fluid delivery device; 110. an extrusion mechanism; 111. a roller body; 112. a helical blade; 113. teeth; 120. a drive mechanism; 130. a delivery line; 131. an extrusion section; 140. a pressure-bearing plane; 200. an incubator.

Detailed Description

In order to more clearly illustrate the technical solutions of the embodiments of the present disclosure, the drawings used in the description of the embodiments will be briefly described below. It is obvious that the drawings in the following description are only examples or embodiments of the present description, and that for a person skilled in the art, the present description can also be applied to other similar scenarios on the basis of these drawings without inventive effort. Unless otherwise apparent from the context, or otherwise indicated, like reference numbers in the figures refer to the same structure or operation.

It should be understood that "system", "apparatus", "unit" and/or "module" as used herein is a method for distinguishing different components, elements, parts, portions or assemblies at different levels. However, other words may be substituted by other expressions if they accomplish the same purpose.

As used in this specification and the appended claims, the terms "a," "an," "the," and/or "the" are not intended to be inclusive in the singular, but rather are intended to be inclusive in the plural, unless the context clearly dictates otherwise. In general, the terms "comprises" and "comprising" merely indicate that steps and elements are included which are explicitly identified, that the steps and elements do not form an exclusive list, and that a method or apparatus may include other steps or elements.

Flow charts are used in this description to illustrate operations performed by a system according to embodiments of the present description. It should be understood that the preceding or following operations are not necessarily performed in the exact order in which they are performed. Rather, the various steps may be processed in reverse order or simultaneously. Meanwhile, other operations may be added to the processes, or a certain step or several steps of operations may be removed from the processes.

The present specification provides a fluid delivery apparatus and a cell perfusion culture device. The fluid conveying equipment comprises an extrusion mechanism, a driving mechanism and a conveying pipeline, wherein the extrusion mechanism comprises a roller body and a spiral blade arranged on the roller body, and the driving mechanism is used for driving the roller body to rotate along the axis of the roller body. Through setting up roll body and helical blade, helical blade can bulldoze the extrusion section along with the roll body is rotatory to for the circulation of fluid in the conveyer pipe provides power, make this liquid conveying equipment's shared space less. The fluid conveying equipment comprises a plurality of conveying pipelines, and different conveying pipelines can be connected with different fluid storage mechanisms and/or different target conveying mechanisms by arranging the plurality of conveying pipelines. In addition, the extrusion mechanism can simultaneously extrude the extrusion sections of the plurality of conveying pipelines, so that the structure of the fluid conveying equipment is more compact, and the fluid conveying efficiency is higher. The fluid transfer device can be used for transferring culture solution in a cell perfusion culture device, and can also be used for transferring fluid in various industries such as biological medicine, food, chemical engineering and the like.

FIG. 1 is a schematic diagram of a fluid delivery device according to some embodiments herein. The liquid delivery device according to the embodiment of the present application will be described in detail with reference to fig. 1, and it should be noted that the following embodiment is only used to explain the present application and should not be construed as limiting the present application.

As shown in fig. 1, the fluid delivery apparatus 100 includes a pressing mechanism 110, a driving mechanism 120, and a delivery pipe 130. The pressing mechanism 110 is used to press the delivery pipe 130. The pressing mechanism 110 includes a roller body 111 and a helical blade 112 provided on the roller body 111. The helical blade 112 may refer to a blade structure that is spirally arranged around the outer circumference of the roller body 111, and the helical blade 112 has a plurality of "teeth" 113 spaced along the axis of the roller body 111 (as indicated by the dotted line 2 in fig. 1). The roller body 111 may be cylindrical, or may be truncated cone or conical. The transfer line 130 is used for flowing a fluid (for example, a culture solution, a drug solution, or the like of a perfusion cell culture system). At least part of the transfer line 130 is parallel to the axis of the roller body 111. The delivery pipe 130 includes a pressing section 131 for being pressed by the screw blade 112. The pressing section 131 refers to a portion of the conveying pipe 131 parallel to the roller body 111 and pressed by the screw blade 112. The fluid delivery device 100 includes a plurality (e.g., two, five, etc.) of delivery lines 130. The extrusion sections 131 of the plurality of conveying pipelines 130 extend along the axial direction of the roller body 111, and the extrusion sections 131 of the conveying pipelines 130 of the plurality of conveying pipelines 130 are arranged at intervals along the axial direction of the roller body 111. The pressing section 131 is in contact with the pressing mechanism 110 and is pressed by the screw blade 112 of the pressing mechanism 110 (i.e., the "teeth" 113 of the screw blade 112). The delivery line 130 may include a pipe body, such as a rubber pipe, a plastic pipe, etc., which can be pressed and deformed by the screw blade 112.

The drive mechanism 120 is for driving the roller body 111 to rotate along the axis of the roller body 111. The drive mechanism 120 may include an electric motor, a motor, or the like. The helical blades 112 can push the pressing section 131 as the roller body 111 rotates, so that the fluid flows in the conveying pipe 130. It will be appreciated that at least two pressure points are provided at which the screw blade 112 presses against the transfer line 130, i.e. at least two "teeth" 113 of the screw blade 112 press against the transfer line 130. For a delivery pipe 130, the portion between the first pressing point and the last pressing point pressed by the spiral blade 112 forms a pressing section 131. For example, when the screw blade 112 has only two "teeth" 113 to press the conveying pipe 130, the pressing section 131 is a portion of the conveying pipe 130 between pressing points of the two "teeth" 113. For another example, when the screw blade 112 has 4 "teeth" to press the delivery pipe 130, the pressing section 131 is a portion between pressing points of the first "tooth" 113 and the fourth "tooth" 113 on the delivery pipe. A cut-off fluid section is formed between two adjacent pressure points, and during the rotation of the helical blade 112 with the roller body 111, the cut-off fluid section between the two adjacent pressure points is pushed, so that the extrusion mechanism 110 provides power for the flow of the fluid in the conveying pipe 130.

In some embodiments, the delivery line 130 may be connected between a fluid storage mechanism (e.g., a culture fluid storage tank of a perfusion culture device) and a target delivery mechanism (e.g., a cell culture dish of a perfusion culture device) to deliver fluid in the fluid storage mechanism to the target delivery mechanism. It is to be understood that only a portion of the transfer line 130 is shown in fig. 1. In some embodiments, delivery line 130 may include an inlet line and an outlet line, both of which may be connected between the fluid storage mechanism and the target delivery mechanism, the inlet line may deliver fluid from the fluid storage mechanism to the target delivery mechanism, and the outlet line may return fluid from the target delivery mechanism to the fluid storage mechanism. At this time, circulation of the fluid may be completed between the delivery line 130, the fluid storage mechanism, and the target delivery mechanism. In some embodiments, the expression segment 131 can be located on the input tube. In other embodiments, the expression segment 131 may be located on the delivery tube. By providing a plurality of delivery lines 130, different delivery lines 130 may be connected to different fluid reservoirs and/or different target delivery mechanisms. In addition, the pressing mechanism 110 simultaneously presses the pressing sections 131 of the plurality of conveying pipelines 130, so that the fluid conveying device 100 is more compact in structure and higher in fluid conveying efficiency.

In some embodiments, the inlet ends of the plurality of extrusion sections 131 are each in communication with a different fluid storage mechanism and the outlet ends of the plurality of extrusion sections 131 are in communication with a different target delivery mechanism. In some embodiments, the inlet ends of the multiple pressing segments 131 are respectively communicated with different fluid storage mechanisms, and the outlet ends of the multiple pressing segments 131 are communicated with the same target conveying mechanism. In some embodiments, the inlet ends of the multiple pressing segments 131 are connected to the same fluid storage mechanism, and the outlet ends of the multiple pressing segments 131 are connected to different target delivery mechanisms.

In some embodiments, fluid delivery apparatus 100 may include a pressure plane 140, and extruded section 131 of one or more delivery conduits 130 may be located between pressure plane 140 and extrusion mechanism 110. The pressure bearing plane 140 is used for bearing the pressing section 131 of the conveying pipeline 130, so that the pressing section 131 and the pressing mechanism 110 form effective pressing fit. The angle between the pressure-bearing plane 140 and the axis of the roller body 111 may be set based on the shape of the roller body 111. For example, when the roller bodies 111 are cylindrical, the bearing plane may be parallel to the axis of the roller bodies 111. For another example, when the roller body 111 has a truncated cone shape, the pressure-receiving plane may be parallel to a generatrix of the roller body 111.

In some embodiments, the helical blades 112 are constant pitch helical blades 112. The pitch of the helical blade 112 is understood as the distance between two "teeth" 113 adjacent along the axis, i.e. the distance a shown in fig. 1. The constant pitch helical blade 112 can be understood as the distance between any two adjacent "teeth" 113 is equal. By providing helical blades 112 as equal helical blades 112, each of the transfer lines 130 within the fluid transfer device 100 may be provided with the same fluid transfer rate.

In some embodiments, the helical blades 112 are variable pitch helical blades 112. The constant pitch helical blades 112 can be understood as the distance between at least two adjacent sets of two "teeth" 113 is different. In some embodiments, the extruded sections 131 of at least two of the plurality of transfer lines 130 have different corresponding thread pitches. The pitch of the extrusion section 131 is understood to be the pitch of the helical blade 112 that extrudes part of the extrusion section 131. If the screw pitches of the extrusion sections 131 of the two conveying pipelines 130 are different, the conveying speeds of the fluids in the two conveying pipelines 130 are different, and the screw pitches of the helical blades 112 corresponding to the extrusion sections 131 of at least two conveying pipelines 130 are different, so that each conveying pipeline 130 in the fluid conveying device 100 has different fluid conveying speeds.

In some embodiments, the pitch of the helical blades 112 may be between 1mm and 50 mm. In some embodiments, the pitch of the helical blades 112 may be between 10mm and 20 mm. In some embodiments, the pitch of the helical blades 112 may be 5mm to 10 mm. In some embodiments, the outer radius of the spiral blade 112 (i.e., distance b in FIG. 1) is between 1mm and 100 mm. In some embodiments, the outer helical radius of the helical blade 112 is between 4mm and 7 mm. In some embodiments, the outer helical radius of the helical blade 112 is between 8mm and 15 mm. The pitch and outer helical radius of the helical blades 112 can affect the delivery rate of the fluid in the delivery conduit 130, and by designing the pitch and outer helical radius, the delivery rate of the fluid in the delivery conduit 130 can be more precisely controlled.

In some embodiments, when the roller body 111 is cylindrical, the ratio of the outer radius of the helical blades 112 to the radius of the roller body 111 is 10:1 to 10: 9. In some embodiments, the ratio of the outer radius of the spiral blade 112 to the radius of the roller body 111 is 10:3 to 10: 7. The ratio of the outer radius of the spiral blade 112 to the radius of the roll body 111 is 2: 1.

In some embodiments, the thickness of the helical blade 112 is between 0.2mm and 20 mm. In some embodiments, the thickness of the helical blade 112 is between 0.5mm and 1 mm. In some embodiments, the thickness of the helical blade 112 is 1mm to 3 mm.

In some embodiments, the surface of the spiral blade 112 contacting the extrusion section 131 and/or the outer surface of the extrusion section 131 is provided with an anti-wear layer (not shown). The wear layer may include a lubrication layer, a protective layer (e.g., a rubber layer), etc. The wear-resistant layer can reduce the friction between the helical blade 112 and the extrusion section 131, and reduce the wear of the conveying pipeline 130.

The embodiment of the present specification also provides a cell perfusion culture device 1000. Fig. 2 is a schematic structural diagram of a perfusion culture system according to some embodiments of the present disclosure, and as shown in fig. 2, a perfusion culture device 1000 includes an incubator 200, and at least a portion of the fluid delivery device according to any of the above embodiments is disposed in the incubator 200. In some embodiments, the squeezing mechanism 110 and the delivery conduit 130 may be disposed within the incubator 200, and the drive mechanism 120 may be disposed outside of the incubator 200. In some embodiments, the squeezing mechanism 110, the driving mechanism 120, and the delivery conduit 130 may all be disposed within the incubator 200. By using the fluid transfer device 100 described above, space utilization within the incubator 200 can be made more rational and the layout within the incubator 200 can be made more compact.

The perfusion culture device disclosed in the present application may bring beneficial effects including but not limited to: (1) the spiral blade can rotate along with the roller body to push and press the extrusion section so as to provide power for the circulation of fluid in the conveying pipe, so that the space occupied by the liquid conveying equipment is smaller; (2) by arranging a plurality of conveying pipelines, different conveying pipelines can be connected with different fluid storage mechanisms and/or different target conveying mechanisms; it is also possible to connect different delivery lines to different fluid reservoirs and/or different target delivery mechanisms. In addition, the extrusion mechanism can simultaneously extrude the extrusion sections of the plurality of conveying pipelines, so that the fluid conveying equipment is more compact in structure and higher in fluid conveying efficiency; (3) the screw pitches of the helical blades corresponding to the extrusion sections of the at least two conveying pipelines are different, so that the conveying pipelines in the fluid conveying equipment have different fluid conveying speeds. It is to be noted that different embodiments may produce different advantages, and in different embodiments, any one or combination of the above advantages may be produced, or any other advantages may be obtained.

The above description is only a preferred embodiment of the present application and should not be taken as limiting the present application, and any modifications, equivalents, improvements, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims (10)

1. A fluid delivery apparatus, comprising:

the extruding mechanism comprises a roller body and a helical blade arranged on the roller body;

the driving mechanism is used for driving the roller body to rotate along the axis of the roller body;

a plurality of conveying pipelines for the circulation of fluid, wherein the conveying pipelines comprise extrusion sections for being extruded by the helical blades; the extrusion sections of the conveying pipelines extend along the axial direction of the roller body, and the extrusion sections of the conveying pipelines are arranged at intervals along the axial direction of the roller body;

wherein the helical blade is configured to push against the extrusion section as the roller body rotates to cause fluid flow in each of the delivery lines.

2. The fluid delivery apparatus according to claim 1, wherein the inlet ends of the plurality of delivery lines are respectively connected to different fluid reservoirs, and the outlet ends of the plurality of delivery lines are respectively connected to different target delivery mechanisms.

3. The fluid delivery apparatus of claim 1, wherein the helical blade is a variable pitch helical blade, and wherein the pitch of the extruded sections of at least two of the plurality of delivery lines is different.

4. The fluid delivery apparatus of claim 1, wherein said helical blade is a constant pitch helical blade.

5. The fluid delivery apparatus according to claim 1, further comprising a pressure-bearing plane, wherein the extruded sections of the plurality of delivery lines are each located between the pressure-bearing plane and the extrusion mechanism.

6. The fluid delivery apparatus according to claim 1, wherein the surface of the helical blade in contact with the extrusion and/or the exterior of the extrusion is provided with a wear layer.

7. The fluid delivery apparatus according to claim 1, wherein the pitch of the helical blade is 1mm to 50 mm; the external spiral radius of the spiral blade is 1 mm-100 mm.

8. The fluid conveying apparatus according to claim 1, wherein the roller body is cylindrical, and a ratio of an outer radius of the helical blade to a radius of the roller body is 10:1 to 10: 9.

9. The fluid delivery apparatus according to claim 1, wherein the helical blade has a thickness of 0.2mm to 20 mm.

10. A perfusion culture device for cells, comprising an incubator in which at least part of the fluid transfer apparatus of any of claims 1-9 is disposed.

Images