CN216639450U

CN216639450U - Special biomembrane device of experiment

Info

Publication number: CN216639450U
Application number: CN202123381131.3U
Authority: CN
Inventors: 张旭阳; 邹玉春; 孙凯瑞; 卢友光; 冯岩; 黄晓宇; 陈遥; 黄玄昊
Original assignee: AFFILIATED STOMATOLOGICAL HOSPITAL FUJIAN MEDICAL UNIVERSITY
Current assignee: AFFILIATED STOMATOLOGICAL HOSPITAL FUJIAN MEDICAL UNIVERSITY
Priority date: 2021-12-30
Filing date: 2021-12-30
Publication date: 2022-05-31
Anticipated expiration: 2031-12-30

Abstract

The utility model relates to the technical field of biological experiment equipment, and discloses a special biological membrane device for an experiment, which comprises: the culture plate comprises a culture plate body with culture holes in an array and a cover body detachably covering the culture plate body, wherein the cover body is adhered or connected with a slide through a plug connector, and the slide is positioned in the corresponding range of the culture holes on the cover body. The cover body is detachably connected with the glass slide, so that the problem that the glass slide is difficult to take and place is solved; through placing a plurality of slides in a culture hole, can obtain a plurality of bacterial biofilm samples through a culture hole, simplify the experiment operation, promote experimental efficiency.

Description

Special biomembrane device of experiment

Technical Field

The utility model belongs to the technical field of biological experiment equipment, and particularly relates to a special biological membrane device for an experiment.

Background

As a basic container for a culture medium, a multi-well plate is a common device used in experiments such as bacterial biofilm culture, and is also called a bacterial biofilm culture plate, and generally includes a plate body, a lid body, and culture wells provided on the plate body. In terms of the number of culture wells, bacterial biofilm culture plates are generally classified into 6-well plates, 12-well plates, 24-well plates, 48-well plates and the like, and generally 6-well plates are the most commonly used bacterial biofilm culture plates for experiments. As the culture holes are generally cylindrical hole grooves, only one culture solution can be added into one culture hole, and in the experiment, one slide is placed in one culture hole, and bacteria are attached to the slide to form a bacterial biofilm experimental sample. When multiple samples of the same bacterial biofilm are required for a given experiment, the same culture solution is added to multiple culture wells, thereby obtaining multiple samples of the same bacterial biofilm. This creates problems: firstly, one 6-pore plate is needed for culturing a bacterial biofilm sample, and particularly, when a control experiment is carried out, a plurality of culture plates are needed for obtaining a plurality of samples, so that the experiment operation time is long and the operation is inconvenient; secondly, the glass slide is placed in the culture hole, so that the glass slide is inconvenient to take and place, the glass slide is easy to break, the bacterial biofilm sample is damaged, and the bacterial biofilm sample with good quality cannot be obtained.

SUMMERY OF THE UTILITY MODEL

The utility model provides a special biological membrane device for experiments, aiming at the problems that the prior six-hole plate is inconvenient to prepare a bacterial biological membrane sample and the like.

The technical scheme of the utility model is realized as follows:

an experimental dedicated biofilm device, comprising:

a culture plate body with culture holes in array and a cover body which can be detachably covered on the culture plate body,

the cover body is bonded or connected with a slide through a plug connector,

the slide is positioned in the corresponding range of the culture hole on the cover body.

As a further scheme of the utility model: at least two slides are arranged in the range corresponding to the culture hole on the cover body.

As a further scheme of the utility model: the glass slide is bonded on the cover body through plasticine.

As a further scheme of the utility model: the number of the plug connectors is consistent with that of the slides, the plug connectors are fixedly connected to the cover body, and the slides are inserted into the plug connectors.

As a further scheme of the utility model: the plug connector comprises an installation part and clamping parts symmetrically arranged on two sides of the installation part, and the front ends of the clamping parts form an outward-expanding plug interface.

As a further scheme of the utility model: the plug connectors are of two types, and a square plug connector or a strip plug connector is selected according to the type of the slide.

As a further scheme of the utility model: the culture plate is provided with a positioning base station, the culture hole is formed in the positioning base station, the positioning base station is provided with two positioning chamfers, the cover body is provided with a cover plate corresponding to the front of the culture plate and a side plate surrounding the cover plate, and the cover body is enclosed into a cover cavity covering the positioning base station through the cover plate and the side plate.

As a further scheme of the utility model: the culture holes are connected with ribs, and the culture holes and the culture plate body are also connected with ribs.

The special experimental biological membrane device has the following beneficial effects:

1. through can dismantle the connection slide on the lid, solve and get the problem of putting the slide difficulty, prevent that the slide is damaged, obtain higher quality bacterial biofilm sample.

2. Through placing a plurality of slides in a culture hole, can obtain a plurality of bacterial biofilm samples through a culture hole, simplify the experiment operation, promote experimental efficiency.

3. The glass slide is connected to the cover body in a bonding or splicing mode, so that the operation is simple and convenient, and the operation time is saved.

4. The inserting port which is expanded outwards is arranged on the inserting piece, so that the inserting of the glass slide is facilitated.

5. The rectangular-shaped glass slide connector is characterized in that the rectangular-shaped connector and the rectangular-shaped connector are arranged, so that the glass slides of different types can be conveniently spliced, for example, the rectangular-shaped connector can be selected for the circular glass slide, the contact area is small, the rectangular-shaped connector can be selected for the square or rectangular glass slide, the contact area is large, and the connection stability is good.

6. The lid covers the location base station, not only plays a sealed effect, and the location nature is high moreover, is convenient for cover and closes.

7. The ribs are arranged between the culture holes, so that the stability and the strength of the culture plate body are improved.

Drawings

FIG. 1 is a schematic view of a biofilm apparatus for experiment according to the present invention;

FIG. 2 is a schematic front view of the culture plate body of FIG. 1;

FIG. 3 is a schematic view of the back side of the culture plate body in FIG. 1;

FIG. 4 is a schematic view of the cover-binding slide of FIG. 1;

FIG. 5 is a schematic view of the cover of FIG. 1 with a square plug;

FIG. 6 is a side view schematic of FIG. 5;

FIG. 7 is a schematic view of the cover of FIG. 1 with an elongated connector;

FIG. 8 is a schematic view of the square socket connector of FIG. 5;

fig. 9 is a schematic view of the elongate connector of fig. 7.

The reference signs are: culture hole 12, culture plate body 1, lid 2, plug connector 3, slide 4, plasticine 5, installation part 31, clamping part 32, interface 33, location base platform 11, location chamfer 13, apron 21, sideboard 22, cover cavity 23, rib 6.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention.

The first embodiment is as follows:

fig. 1 to 4 are schematic views for explaining one embodiment of an experimental-dedicated biofilm device of the present invention. The illustrated experimental-specific biofilm apparatus includes: a culture plate body 1 and a cover body 2 covering the culture plate body 1 are shown in FIG. 1. As shown in fig. 2 to 3, the front surface of the culture plate body 1 is provided with a protruding positioning base 11, the two positioning base are in a ladder shape, and two corners on one side of the positioning base 11 are chamfered to form two positioning chamfers 13, so that the cover body 2 can be conveniently covered and positioned. Six culture holes 12 are arranged on a positioning base platform 11 in an array mode, the culture holes 12 are cylindrical with bottoms, a connecting plate surface is arranged between each culture hole 12 and the positioning base platform 11, gaps are formed among the six culture holes 12, and ribs 6 are connected between every two adjacent culture holes 12. The back of the culture plate body 1 is opposite to the front, a connecting plate surface is arranged between the bottom surfaces of the six culture holes 12, and ribs 6 are connected between the culture holes 12 and the culture plate body 1, so that the culture plate body 1 can meet the requirement of stability while reducing weight. The lid 2 has the apron 21 that openly corresponds with the culture plate body 1 and encircles the sideboard 22 of apron 21 four sides, and lid 2 encloses into the cover chamber 23 that covers in location base station 11 through apron 21 and sideboard 22, and the cover chamber 23 size holds location base station 11 just, possesses better sealed effect. When the cover 2 is covered on the culture plate 1, the side plate 22 of the cover 2 is attached to the outer edge of the positioning base 11, and the bottom of the side plate 22 is attached to the culture plate 1. The cover plate 21 of the cover body 2 corresponding to the front surface of the culture plate body 1 is also provided with a plurality of annular nicks, the annular nicks correspond to the culture holes 12 on the culture plate body 1 one by one, and the diameter of the annular nicks is consistent with that of the culture holes 12.

Selecting a glass slide 4 which is small in size and can be placed in the culture hole 12, pulling a small rubber clay 5 to bond the glass slide 4 in the annular nick of the cover plate 21, and ensuring that the outer edge of the glass slide 4 does not exceed the range of the annular nick. According to the experiment requirement, one glass slide 4 or at least two glass slides 4 can be adhered in one annular nick, so that one or more bacterial biofilm samples can be obtained in one culture hole 12, and the experiment requirement is met.

Example two:

fig. 1 to 3 and 5 to 9 are schematic views for explaining another embodiment of the experimental-dedicated biofilm device of the present invention. As shown in fig. 1 to 3, the experimental biofilm device includes: cultivate the plate body 1 and cover lid 2 on cultivateing the plate body 1, cultivate the plate body 1 and openly be equipped with convex location base station 11, both are the echelonment, carry out the chamfer on two angles of location base station 11 one side, form two location chamfers 13, make things convenient for lid 2 to cover the location. Six culture holes 12 are arranged on a positioning base platform 11 in an array mode, the culture holes 12 are cylindrical with bottoms, a connecting plate surface is arranged between each culture hole 12 and the positioning base platform 11, gaps are formed among the six culture holes 12, and ribs 6 are connected between every two adjacent culture holes 12. The back of the culture plate body 1 is opposite to the front, a connecting plate surface is arranged between the bottom surfaces of the six culture holes 12, and ribs 6 are connected between the culture holes 12 and the culture plate body 1, so that the culture plate body 1 can meet the requirement of stability while reducing weight. The lid 2 has the apron 21 that openly corresponds with the culture plate body 1 and encircles the sideboard 22 of apron 21 four sides, and lid 2 encloses into the cover chamber 23 that covers in location base station 11 through apron 21 and sideboard 22, and the cover chamber 23 size holds location base station 11 just, possesses better sealed effect. When the cover 2 is covered on the culture plate 1, the side plate 22 of the cover 2 is attached to the outer edge of the positioning base 11, and the bottom of the side plate 22 is attached to the culture plate 1. The cover plate 21 of the cover body 2 corresponding to the front surface of the culture plate body 1 is also provided with a plurality of annular nicks, the annular nicks correspond to the culture holes 12 on the culture plate body 1 one by one, and the diameter of the annular nicks is consistent with that of the culture holes 12.

As shown in fig. 5 to 9, one or at least two connectors 3 are fixedly connected in the annular notch of the cover plate 21, and the fixed connection may be an adhesive or other connection method. Selecting a slide 4 which has a smaller size and can be placed in the culture hole 12, inserting the slide 4 on the plug connector 3 by using tweezers, and ensuring that the outer edge of the slide 4 does not exceed the range of the annular nick. According to the experiment requirement, one or at least two plug connectors 3 can be arranged in one annular nick, so that one or more bacterial biofilm sample slides 4 can be obtained in one culture hole 12, and the experiment requirement is met.

As shown in FIGS. 8 to 9, there are two types of connectors 3, and a square connector 3 or a strip connector 3 is selected depending on the type of the slide 4. For example, the square plug-in unit 3 can be used as the circular slide 4, and the contact area between the circular slide 4 and the plug-in unit 3 is small, so that only the plug-in unit 3 with a small size is required. And the slide 4 is opposite to the plug connector 3 and is inserted into the plug connector 3 from bottom to top. Rectangular connector clip 3 can be selected to square or rectangle slide 4, and square or rectangle slide 4 is big with the area of contact of connector clip 3, and the connection steadiness is good, and slide 4 inserts in connector clip 3 from connector clip 3 one side.

The plug-in unit 3 comprises a mounting portion 31 and clamping portions 32 symmetrically arranged at two sides of the mounting portion 31, the mounting portion 31 is fixedly connected in the annular nick of the cover plate 21, and the clamping portions 32 can also be made of some elastic materials. The front ends of the two clamping portions 32 form an outwardly flared socket 33 for facilitating the insertion of the slide 4. The opposite surfaces of the two clamping parts 32 of the square plug-in connector 3 are arc-shaped, so that a clamping groove with a narrow middle part and wide two sides is formed, and the circular slide 4 can be better clamped to prevent the circular slide from falling. Two clamping parts 32 one side of rectangular shape plug connector 3 are equipped with the interface 33 that expands outward, and slide 4 is from being equipped with interface 33 one side insert in the plug connector 3, and clamping part 32 is longer, and slide 4 not only receives the clamping force effect of two clamping parts 32, but also because area of contact is big, and the frictional force between slide 4 and the clamping part 32 is great, prevents that slide 4 from dropping.

Thus, the object of the present invention is accomplished.

The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions, improvements, etc. within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An experimental dedicated biofilm device, comprising:

the cover body is bonded or connected with a slide through a plug connector,

2. The special biofilm device for experiments according to claim 1, wherein at least two slides are arranged in the range corresponding to the culture hole on the cover body.

3. The device of claim 2, wherein the slide is adhered to the cover by a plasticine.

4. The special experimental biofilm device of claim 2, wherein the number of the plug connectors is the same as that of the slides, the plug connectors are fixedly connected to the cover body, and the slides are plugged into the plug connectors.

5. The special experimental biofilm device according to claim 4, wherein the plug connector comprises a mounting part and clamping parts symmetrically arranged on two sides of the mounting part, and an outward-expanding socket is formed at the front ends of the two clamping parts.

6. The experimental biofilm unit according to claim 5, wherein said connectors are two types, and a square connector or a strip connector is selected according to a type of a slide.

7. The special biofilm device for experiments according to any one of claims 1 to 6, wherein a positioning base is arranged on the culture plate body, the culture holes are arranged on the positioning base, two positioning chamfers are arranged on the positioning base, the cover body is provided with a cover plate corresponding to the front surface of the culture plate body and a side plate surrounding the cover plate, and the cover body is enclosed into a cover cavity covered on the positioning base through the cover plate and the side plate.

8. The special biofilm device for experiments according to claim 7, wherein ribs are connected between the culture holes, and ribs are also connected between the culture holes and the culture plate body.