CN216630667U - Antigen emulsification fixing device for immunization - Google Patents

Abstract

The utility model provides an antigen emulsification fixing device for immunization, relates to the technical field of experimental instruments, mainly aims to solve the problems that the traditional manual emulsification and blending operation is poor in accuracy and has certain risks in operation, and provides a simpler, more convenient and safer emulsification fixing device. The antigen emulsification fixing device for immunization comprises a base, a support frame and a fixing platform, wherein the base plays a role in supporting the device; the supporting frame is vertically arranged on the base; the fixed station has arranged two in proper order along the plumb line direction, is located the top the fixed station level sets up and through an orientation support frame direction slope decurrent hypotenuse with the top fixed connection of support frame, be located the below the fixed station pass through fixed connection's the lantern ring with the support frame links to each other and can follow the direction of height sliding movement of support frame is in order to adjust two distance between the fixed station. The utility model is used for providing a fixing device for emulsification operation, which is convenient to operate and safer.

Description

Antigen emulsification fixing device for immunization

Technical Field

The utility model relates to the technical field of experimental instruments, in particular to an antigen emulsification fixing device for immunization.

Background

Antigen immunization is a key step in immunological detection techniques. Before antigen immunization, the antigen is generally required to be emulsified with an immunological adjuvant such as Freund's adjuvant to prepare a "water-in-oil" emulsion, and then injected for immunization. Conventional antigen emulsification methods include milling, syringe mixing, and ultrasonic mixing. The grinding method requires heating the adjuvant and placing a proper amount of the adjuvant into a sterile glass mortar, cooling, slowly dropping an equal volume of antigen solution, grinding in the same direction while dropping, and the speed of dropping the antigen is slow. After the antigen is added completely, grinding is continued for a period of time to make the emulsion become milky viscous water-in-oil emulsion. This method results in a large amount of emulsion adhering to the walls of the mortar with a large loss of antigen. The syringe mixing method needs to suck equivalent Freund's adjuvant and antigen solution into two syringes respectively, connect the two syringes with a thin rubber tube, drain air, and then push the needle tube alternately until a viscous emulsion is formed. This method has a problem that complete emulsification is difficult, and the emulsifier prepared by this method needs to be identified before use. The ultrasonic mixing method is to add the antigen and the adjuvant into a centrifuge tube according to the required amount, place the centrifuge tube on an ultrasonic crusher and perform ultrasonic repeated treatment for a plurality of times to obtain the antigen-adjuvant-antibody composite membrane. The advantages of this method are simplicity, rapidity, and material savings, but ultrasound readily excites some of the free radicals, producing unknown damage to the antigen. Therefore, the above methods are all deficient to various degrees and difficult to meet the needs of experimental work.

In order to solve the problems and achieve efficient and rapid obtaining of emulsified antigens, a handheld electric stirrer is mostly adopted in a laboratory at present to be matched with a special stirring paddle to achieve uniform mixing and emulsification treatment of an antigen mixed solution to be emulsified. However, the hand-held blender method requires a person to directly hand and move the blender and the container, and the operation accuracy is low, which results in prolonged emulsification time; meanwhile, due to the instability of hands, the stirring paddle is easy to touch the wall of the injector to cause damage of the instrument, and certain danger is caused.

In order to solve the above problems, it is necessary to develop a novel antigen emulsification and fixation device for immunization.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide an antigen emulsification fixing device for immunization, which aims to solve the technical problems that the emulsification operation of immune antigens is not simple enough and certain dangerousness exists in the prior art. The technical effects that can be produced by the preferred technical scheme in the technical schemes provided by the utility model are described in detail in the following.

In order to achieve the purpose, the utility model provides the following technical scheme:

the utility model provides an antigen emulsification fixing device for immunization, which comprises:

the base supports the device;

the supporting frame is vertically arranged on the base;

the fixed station, two have been arranged in proper order along the plumb line direction to the fixed station, be located the top the fixed station level sets up and through an orientation support frame direction slope decurrent hypotenuse with the top fixed connection of support frame, be located the below the fixed station pass through fixed connection's the lantern ring with the support frame links to each other and can follow the direction of height sliding movement of support frame is in order to adjust two distance between the fixed station.

Under the effect of two fixed stations of arranging from top to bottom, treat that the liquid of emulsification and the agitator that is used for carrying out emulsification treatment all can be fixed on corresponding position through the fixed station, and the agitator lower extreme stretches into in treating the liquid of emulsification and starts to the realization is to the emulsification treatment of immune antigen. Owing to the height-adjustable fixed table, the device can be suitable for stirrers and containers of different specifications and also for containers which contain small or large amounts of the liquid to be treated.

On the basis of the technical scheme, the utility model can be further improved as follows.

As a further improvement of the utility model, the fixing table comprises a fixing plate, the middle part of the fixing plate is provided with a hollow structure, and an object to be fixed penetrates through the hollow structure to be connected with the fixing plate, so that the object to be fixed is fixed through the hollow structure, and the subsequent emulsification process can be carried out efficiently and safely.

The hollow structure can realize the fixation of the stirrer and the container with the sizes matched with the hollow structure.

As a further improvement of the utility model, the fixed station further comprises two limiting plates, and the two limiting plates are consistent in structure and are symmetrically arranged;

the hollow portion of fixed plate is the rectangle, and the guide slot has all been seted up to its relative both sides that set up, two the guide slot sets up along horizontal plane direction parallel, the limiting plate passes through the guide slot with the fixed plate links to each other and can follow the length direction sliding movement of guide slot, two of relative setting the limiting plate middle part forms the space that is used for fixed article.

The size of the space that is used for fixed agitator or container can effectively be adjusted through above-mentioned two limiting plates to make this fixed station can be applicable to the agitator and the container of multiple not unidimensional.

As a further improvement of the utility model, one side of the limiting plate facing to the middle part of the fixing plate is provided with a partial curved surface.

This curved surface structure can help the outer wall of the better laminating agitator of limiting plate or container, realizes better fixed effect.

As a further improvement of the utility model, the fixing table further comprises an adjusting member, at least part of which is located between the fixing plate and the limiting plate and is used for adjusting the distance between the two limiting plates.

The adjusting piece can help to adjust the distance of the limiting plate on the fixing plate.

As a further improvement of the utility model, the adjusting member is a spring;

or the adjusting piece is an adjusting screw arranged on the fixing plate in a penetrating mode, the adjusting screw penetrates through the fixing plate and is connected with the limiting plate, and the limiting plate can be driven to slide and move relative to the guide groove by rotating the adjusting screw.

As a further improvement of the utility model, the outer surface of the guide groove is provided with a scale.

The position of the limiting plate relative to the fixing plate can be determined through the scales, so that the article fixed by the fixing table is always located in the center of the fixing table and cannot be deviated.

As a further improvement of the utility model, the lantern ring is sleeved on the peripheral side of the support frame and connected with the support frame, bolt holes are arranged on two opposite sides of the lantern ring, and bolts penetrating through the bolt holes can be mutually abutted with the outer side wall of the support frame.

The position of the lantern ring on the support frame can be adjusted by the aid of the bolts, and accordingly the distance between the two fixing tables can be adjusted.

As a further improvement of the utility model, at least part of the cross-sectional structure of the support frame is a circular or H-shaped structure.

As a further improvement of the utility model, the lantern ring is of an open structure.

Compared with the prior art, the antigen emulsification fixing device for immunization provided by the preferred embodiment of the utility model has the advantages that the stirrer is fixed on the fixing platform fixed on the top of the device, the container for containing liquid is fixed on the fixing platform capable of moving up and down, and the centers of the two fixing platforms are superposed. When in use, the free end of the stirrer positioned at the upper end can extend into the container and directly act on liquid so as to realize the emulsification treatment of the immune antigen; limiting plates and adjusting pieces in the fixed table can help corresponding stirrers and containers to be fixed and positioned, and therefore the stirrers and the containers can be guaranteed to be located in the center of the fixed table all the time.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a side view of an antigen emulsifying fixation device for immunization according to the present invention;

FIG. 2 is a schematic view of the structure of the fixing table of FIG. 1 at the upper end;

fig. 3 is a schematic cross-sectional view of the fixing plate of fig. 2;

FIG. 4 is a schematic view of the structure of the lower fixed table of FIG. 1;

FIG. 5 is a schematic structural view showing a second example of the fixing table at the upper end in the antigen emulsifying and fixing apparatus for immunization according to the present invention;

FIG. 6 is a schematic structural view showing a second example of a holding stage at the lower end of the antigen emulsifying and fixing apparatus for immunization according to the present invention;

FIG. 7 is a schematic diagram of the use of the antigen emulsifying immobilization device for immunization according to the present invention.

In the figure: 1. a base; 2. a support frame; 3. a fixed table; 31. a fixing plate; 311. a hollow structure; 312. a guide groove; 313. calibration; 32. a limiting plate; 33. an adjustment member; 4. a bevel edge; 5. a collar; 51. bolt holes.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely exemplary of the utility model, and not restrictive of the full scope of the utility model. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations and positional relationships based on those shown in the drawings, and are used only for convenience of description and simplicity of description, and do not indicate or imply that the equipment or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be considered as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. The specific meaning of the above terms in the present invention can be understood as appropriate to those of ordinary skill in the art.

FIG. 1 is a side view of the antigen emulsifying immobilization device for immunization according to the present invention; as can be seen from the figure, the device comprises a base, a support frame and two fixing tables from bottom to top in sequence, wherein the number of the fixing tables is two, and the fixing tables positioned below can move up and down along the axis direction of the support frame through a lantern ring and are fixed at corresponding positions of the support frame; the fixing platform positioned above is fixedly connected with the supporting frame through a bevel edge; in addition, two fixed stations that set up from top to bottom are located the same side of support frame and the midline of both is located same plumb line.

FIG. 2 is a schematic view of the structure of the upper fixing table of FIG. 1; it can be seen that the whole fixed plate in this fixed station is square structure, and there is a rectangle hollow structure in its inside, and symmetrical arrangement has two limiting plates and the regulating part corresponding with these two limiting plates in this hollow structure, and this regulating part is for wearing to establish the adjusting screw (the screw thread is not drawn in the picture) on the fixed plate this moment, and this adjusting screw links to each other with the limiting plate with fixed plate threaded connection and its one end that is located hollow structure, along with adjusting screw inserts hollow structure gradually, two limiting plates also are close to gradually under adjusting screw's promotion. In addition, the fixed plate is also provided with scales, and the distance between the two limit plates and the inner wall of the fixed plate can be seen through the scales.

FIG. 3 is a schematic cross-sectional view of FIG. 2; the structure of the guide groove in the fixing plate can be clearly seen from this angle.

FIG. 4 is a schematic view of the structure of the lower end mounting plate of FIG. 1; in comparison with fig. 2, the structural difference between the two is mainly the collar portion, by means of which the fixed table can be connected to the support frame.

FIG. 5 is a schematic structural view of a second embodiment of a fixing table at the upper end of the antigen emulsifying and fixing apparatus for immunization according to the present invention; compared with the prior art in fig. 2, the structure of the adjusting piece is changed from an adjusting screw to a spring, and meanwhile, in order to ensure the moving stability of the limiting plate, one limiting plate is connected with the fixing plate through two springs positioned on the same side.

FIG. 6 is a schematic structural view showing a second example of a fixing table at a lower end of the antigen emulsifying and fixing device for immunization according to the present invention; in this case, the adjusting member in the fixed stand is also a spring.

FIG. 7 is a schematic diagram of the use of the antigen emulsification fixture according to the present invention; in use, the upper fixing table can be used for fixedly mounting the stirrer, and the lower fixing table can be used for fixing a container containing the liquid to be emulsified, such as a test tube, a syringe, an EP tube and the like, wherein the lower end of the stirrer can penetrate into the container and is directly contacted with the liquid in the container.

The technical solution of the present invention will be specifically described below with reference to the accompanying drawings.

The utility model provides an antigen emulsification fixing device for immunization, which can be used for fixing an electric stirrer and a container containing liquid to be emulsified so as to realize rapid and safe emulsification treatment of the container.

Specifically, the device comprises a base 1, a support frame 2 and a fixing table 3, as shown in fig. 1, wherein the base 1 is fixedly connected with the support frame 2, the base 1 plays a role in fixing, and the support frame 2 can support the fixing table 3 to a certain height.

The quantity of above-mentioned fixed station 3 is two and two fixed stations 3 all arrange from top to bottom along the perpendicular line direction: the fixed station 3 that is located the top sets up and through one 2 direction inclination hypotenuses 4 and the top fixed connection of support frame 2 of inclining downwards towards the support frame, and the fixed station 3 that is located the below links to each other with support frame 2 through fixed connection's lantern ring 5 and can follow the direction of height sliding movement of support frame 2 in order to adjust the distance between two fixed stations 3.

Under the effect of two fixed stations 3 that arrange from top to bottom, the liquid that treats the emulsification and the agitator that is used for carrying out emulsification all can be fixed on corresponding position through fixed station 3, and the agitator lower extreme stretches into in the liquid that treats the emulsification and starts to the realization is to the emulsification of immune antigen. Owing to the height-adjustable mounting 3, the device can be adapted to stirrers and containers of different sizes, and also to containers which hold small or large quantities of the liquid to be treated. The use of the device is schematically shown in figure 7.

It should be noted that, in order to ensure the stability of the whole device, the bevel edge 4 and the supporting frame 2 may be provided as an integral structure. Or, the fixing table 3 for fixing the stirrer, the bevel edge 4 and the support frame 2 are all of an integrated structure, and at the moment, the support frame 2 and the fixing table 3 can form an inverted L-shaped structure.

In order to facilitate the up-and-down sliding movement of the other fixing table 3 relative to the support frame 2, the cross section of the support frame 2 in the vertical direction may be any one of circular, rectangular, or I-shaped (i.e., H-shaped).

Specifically, the lantern ring 5 is sleeved on the periphery of the support frame 2 and connected with the support frame 2, and at the moment, the corresponding fixing table 3 is connected with the support frame 2 through the lantern ring 5 structure. In order to realize the fixing and sliding movement of the lantern ring 5 and the support frame 2, bolt holes 51 are arranged on two opposite sides of the lantern ring 5, and bolts penetrating through the bolt holes 51 can be mutually abutted with the outer side wall of the support frame 2 to fix the corresponding fixing table 3.

That is, when the bolt abuts against the outer side wall of the support frame 2, the collar 5 can be fixed on the support frame 2 by the friction force; when the bolt is loosened and does not abut against the support frame 2, the collar 5 can be moved up and down in the axial direction of the support frame 2 by an external force. The position of the lantern ring 5 on the support frame 2 can be adjusted by the aid of the bolts, and accordingly the distance between the two fixing platforms 3 can be adjusted.

As an alternative embodiment, the collar 5 is an open structure, as shown in fig. 4.

The fixed table 3 is fixedly connected with the bevel edge 4 or the lantern ring 5 through a strip-shaped connecting structure.

Example 1:

the utility model provides an antigen emulsification fixing device for immunization, which comprises a base 1, a support frame 2 and two fixing platforms 3 which are arranged up and down along the vertical line direction.

The fixing table 3 comprises a fixing plate 31, the fixing plate 31 is provided with a hollow structure 311 in the middle, and the object to be fixed passes through the hollow structure 311 and is connected with the fixing plate 31, so that the object to be fixed is fixed through the hollow structure 311, and the subsequent emulsification process can be efficiently and safely carried out.

The hollow structure 311 can fix the stirrer and the container with the size matched with the hollow structure 311. The fixed table 3 can only be used for fixing devices and containers of a certain size.

Example 2:

the present embodiment 2 is different from embodiment 1 in that: as shown in fig. 2-4, the stationary table 3 can now be adapted to different sizes of agitators and containers.

In order to improve the adaptability of the fixed table 3, the fixed table 3 further comprises two limiting plates 32 with the same shape and size, and the two limiting plates 32 are symmetrically arranged; the hollow portion of fixed plate 31 is the rectangle, and guide slot 312 has all been seted up to its relative both sides that set up, and two guide slot 312 are along horizontal plane direction parallel arrangement, and limiting plate 32 passes through guide slot 312 and links to each other with fixed plate 31 and can follow the length direction sliding movement of guide slot 312, and the relative two limiting plate 32 middle parts that set up form the space that is used for fixed article. That is, the hole formed between the two stopper plates 32 is a hole for fixing the agitator or the vessel.

The size of the space for fixing the stirrer or the container can be effectively adjusted by the two limiting plates 32, so that the fixing table 3 can be suitable for stirrers and containers of various sizes.

It should be noted that the limiting plate 32 can better fix the object. As an optional implementation manner, one side of the limiting plate 32, which faces the middle of the fixing plate 31, has a partial curved surface, and the curved surface structure can help the limiting plate 32 to better adhere to the outer wall of the stirrer or the container, so as to achieve a better fixing effect; alternatively, as an alternative embodiment, a buffer is present on the opposite side of the two limit plates 32, and the buffer may be made of a rubber material with certain elasticity.

As an alternative embodiment, the fixing table 3 further includes an adjusting member 33, and at least a portion of the adjusting member 33 is located between the fixing plate 31 and the limiting plate 32 and is used for adjusting the distance between the two limiting plates 32.

The adjusting member 33 can help to adjust the distance of the position limiting plate 32 on the fixing plate 31.

As an alternative embodiment, the adjusting member 33 is an adjusting screw passing through the fixing plate 31, the adjusting screw passes through the fixing plate 31 and is connected to the limiting plate 32, and the adjusting screw can be inserted into or withdrawn from the hollow structure 311 and pushes the limiting plate 32 to slide relative to the guide groove 312 in the process along with the rotation of the adjusting screw, so as to adjust the distance between the two limiting plates 32.

As an alternative embodiment, the outer surface of the guide groove 312 is provided with a scale 313.

The position of the position-limiting plate 32 relative to the fixing plate 31 can be specified by the scale 313, so that the article fixed by the fixing table 3 is always positioned at the center thereof and does not deviate.

In addition, the outer edges of the two fixing bases 3 are not necessarily identical in shape, and may have a rectangular or circular configuration for aesthetic reasons.

Example 3:

the present embodiment 3 is different from embodiment 2 in that: as shown in fig. 5 to 6, the adjusting member 33 is a spring structure, and any one of the limiting plates 32 is connected to the fixing plate 31 through the same number of symmetrically arranged spring structures.

It should be noted that the spring structure is located on the same side of the respective limit plate 32.

Compare with traditional equipment for emulsification, this fixing device adopts vertical design, can effectively improve the stability of equipment when using, and the fixed station 3 that is located the upper end can be applicable to not unidimensional electric agitator, and the fixed station 3 that is located the lower extreme can be applicable to containers such as not unidimensional syringes. Meanwhile, the electric stirrer is fixedly arranged, so that manual handheld operation is not needed when the electric stirrer is used, and the health of operators can be protected on the premise of realizing automatic emulsification. In addition, the collar 5 may help to adjust the spacing between the two fixed stations 3, thereby helping to control the emulsification time and the degree of emulsification of the liquid.

The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.

Claims (8)

1. Antigen emulsification fixing device for immunization characterized by, includes:

a base (1);

the supporting frame (2), the said supporting frame (2) is set up on the said base (1) vertically;

the device comprises fixing tables (3), wherein two fixing tables (3) are sequentially arranged along the vertical line direction, the fixing table (3) positioned above is horizontally arranged and is fixedly connected with the top end of the support frame (2) through a bevel edge (4) which inclines downwards towards the support frame (2), and the fixing table (3) positioned below is connected with the support frame (2) through a fixedly connected lantern ring (5) and can slide and move along the height direction of the support frame (2) so as to adjust the distance between the two fixing tables (3);

the fixing table (3) comprises a fixing plate (31) and a limiting plate (32), a hollow structure (311) is arranged in the middle of the fixing plate (31), and an article to be fixed penetrates through the hollow structure (311) to be connected with the fixing plate (31); the number of the limiting plates (32) is two, and the two limiting plates (32) are consistent in structure; the well portion of fixed plate (31) is the rectangle, and guide slot (312), two have all been seted up to its relative both sides that set up guide slot (312) along horizontal plane direction parallel arrangement, limiting plate (32) pass through guide slot (312) with fixed plate (31) link to each other and can follow the length direction sliding movement of guide slot (312), relative two that set up limiting plate (32) middle part forms the space that is used for fixed article.

2. The emulsifying fixation device for antigens for immunity according to claim 1, characterized in that the side of the restriction plate (32) facing the middle of the fixation plate (31) has a partial curved surface.

3. The emulsifying fixation device for antigens for immunization according to claim 1, characterized in that said fixed bench (3) further comprises an adjusting element (33), at least part of said adjusting element (33) being located between said fixed plate (31) and said plate (32) for adjusting the distance between the two plates (32).

4. The emulsifying fixation device of an antigen for immunization according to claim 3, characterized in that the adjustment member (33) is a spring;

or, regulating part (33) is for wearing to establish adjusting screw on fixed plate (31), adjusting screw passes fixed plate (31) with limiting plate (32) link to each other, through rotating adjusting screw can drive limiting plate (32) for guide slot (312) sliding movement.

5. The emulsification fixture of claim 1, wherein the outer surface of the channel (312) is provided with a scale (313).

6. The antigen emulsifying and fixing device for immunization according to claim 1, wherein the sleeve ring (5) is sleeved on the outer peripheral side of the support frame (2) and connected with the support frame (2), bolt holes (51) are arranged on two opposite sides of the sleeve ring (5), and bolts penetrating through the bolt holes (51) can abut against the outer side wall of the support frame (2).

7. The emulsification fixation device according to claim 6, wherein at least a portion of the scaffold (2) has a circular or H-shaped cross-section.

8. The emulsifying fixation device for antigens for immunity according to claim 6, characterized in that the collar (5) is an open structure.

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