CN216630410U

CN216630410U - Agitating unit is used in preparation of protease K

Info

Publication number: CN216630410U
Application number: CN202220209226.2U
Authority: CN
Inventors: 阎永贞; 徐廉政
Original assignee: Shandong Ruiyin Bioengineering Co ltd
Current assignee: Shandong Ruiyin Bioengineering Co ltd
Priority date: 2022-01-26
Filing date: 2022-01-26
Publication date: 2022-05-31
Anticipated expiration: 2032-01-26

Abstract

The utility model discloses a stirring device for preparing protease K, which relates to the technical field of protease K preparation equipment and comprises a base, wherein a supporting block is fixedly arranged at the top end of the base; the utility model has the beneficial effects that: through the cooperation of filtering component, lifting unit and install bin, need not to dismantle the churn and can take out the sediment, the staff of being convenient for clears up the sediment, reduces intensity of labour, through setting up the install bin, when being convenient for clear up the sediment, the pH value of suspension is convenient for survey and adjust, and is more convenient, thereby the staff adds the pH value of dilute hydrochloric acid solution regulation suspension through the opening on install bin top.

Description

Agitating unit is used in preparation of protease K

Technical Field

The utility model relates to a stirring device, in particular to a stirring device for preparing protease K, and belongs to the technical field of protease K preparation equipment.

Background

Proteinase K is an important serine proteinase secreted by Candida albicans, has a very wide substrate spectrum and has high proteolytic activity. Proteinase K is active over a wide pH range (pH 4-12.5). And when the proteinase K is prepared, 25% of saturated ammonium sulfate is required to be added into the filtrate for precipitation, the process is continuously stirred, after the ammonium sulfate is completely added, the pH value of the suspension is measured, the pH value is adjusted to 5.0 by using a dilute hydrochloric acid solution, the adjusted suspension is taken out, and the suspension is centrifuged and the supernatant is collected.

The stirring device for preparing the protease K is not convenient for cleaning sediment at the bottom of the stirring cylinder, the stirring cylinder needs to be disassembled after liquid is taken, the stirring cylinder is poured again, and the sediment needs to be taken out before next stirring, so that the working strength of workers is increased, time and labor are wasted, and the stirring device for preparing the protease K is provided.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a stirring device for preparing proteinase K, which solves the problem that the existing stirring device for preparing proteinase K in the background art is inconvenient to clean sediment at the bottom of a stirring container.

In order to achieve the purpose, the utility model provides the following technical scheme: the utility model provides a agitating unit is used in protease K preparation, the on-line screen storage device comprises a base, the fixed supporting shoe that is equipped with in top of base, the fixed churn that is equipped with in top of supporting shoe, the fixed agitator motor that is equipped with in one end of churn, agitator motor's the fixed transfer line that is equipped with of transmission shaft, the fixed surface of transfer line is equipped with spiral stirring leaf, the fixed install bin that is equipped with of the other end of churn, the inside of install bin is equipped with filtering component, the bottom mounting of install bin is equipped with the motor case, the top of motor case is equipped with lifting unit.

Preferably, the filter assembly comprises a filter box, the filter box is arranged at the bottom inside the installation box in a sliding mode, a plurality of filter holes are formed in the bottom of the filter box, a sinking assisting block and a first connecting plate are fixedly arranged at the top of the filter box respectively, the surface of the sinking assisting block and the surface of the first connecting plate are connected with the inner wall of the installation box in a sliding mode, an auxiliary block is fixedly arranged at the top end of the first connecting plate, and a through groove is formed in the middle of the auxiliary block.

Preferably, the lifting assembly comprises a lifting motor, the lifting motor is a forward and reverse rotating motor, the inner wall of the top end of the motor box is fixedly arranged on the lifting motor, a transmission lead screw is fixedly arranged on a transmission shaft of the lifting motor, a moving pipe is connected to the surface of the transmission lead screw through lead screw nuts and threads, a limiting block and two second connecting plates are fixedly arranged on the surface of the moving pipe respectively, two supporting rods are fixedly arranged on the top ends of the second connecting plates, an auxiliary plate which slides in the through groove is fixedly arranged on the top ends of the two supporting rods, a limiting plate is fixedly arranged on the top end of the auxiliary plate, one side of the limiting plate is slidably connected with one side of the auxiliary block, a threaded hole is formed in the surface of the auxiliary plate, a screw rod is connected to the inner wall of the threaded hole through threads, and a power-assisting disc is fixedly arranged on the top end of the screw rod.

Preferably, a baffle is fixedly arranged on one side of the top end of the motor box, and a limiting groove which slides with the limiting block is formed in one side of the baffle.

Preferably, the surface of the mixing drum is respectively and fixedly provided with a liquid inlet pipe, a medicine injection pipe and a liquid outlet pipe, the surface of the liquid inlet pipe is fixedly provided with a first electric valve, the surface of the medicine injection pipe is fixedly provided with a second electric valve, and the surface of the liquid outlet pipe is fixedly provided with a third electric valve.

Preferably, the inside of churn is equipped with the stable dish, the fixed surface of stable dish is equipped with a plurality of connecting rod, a plurality of the one end of connecting rod is the fixed inner wall that sets up at the churn all, the stable dish rotates through sealed bearing and transfer line to be connected, the through-hole has been seted up to one side of install bin, the install bin is linked together through the inside of through-hole and churn.

Preferably, the fixed flush mounting plate that is equipped with in front of supporting shoe, flush mounting plate's surface is equipped with agitator motor switch, first electric valve switch, second electric valve switch, third electric valve switch and driving motor switch respectively, agitator motor, first electric valve, second electric valve, third electric valve and elevator motor are respectively through agitator motor switch, first electric valve switch, second electric valve switch, third electric valve switch and driving motor switch and external power source electric connection.

Compared with the related art, the stirring device for preparing the protease K provided by the utility model has the following beneficial effects:

1. through the matching of the filtering component, the lifting component and the mounting box, the sediment can be taken out without disassembling the mixing drum, so that workers can clean the sediment conveniently, the labor intensity is reduced, the lifting motor is started through a lifting motor switch, the lifting motor drives the transmission lead screw to rotate, the transmission lead screw rotates to drive the moving pipe to move upwards, the support rod is further driven to move upwards, the support rod drives the auxiliary plate to move upwards, the auxiliary plate drives the filtering box to move upwards, and the filtering box slides out of the mounting box;

2. through setting up the install bin, be convenient for clear up when deposiing, be convenient for survey and adjust the pH value of suspension, it is more convenient, thereby the staff adds the pH value of dilute hydrochloric acid solution regulation suspension through the opening on install bin top, thereby the pH value of liquid survey suspension is got to the opening through the install bin top, through agitator motor, the transfer line, the cooperation of churn and spiral stirring leaf, will deposit propelling movement to install bin on one side when can stirring, be favorable to clearing up the sediment, set up connecting rod and stabilizer disc simultaneously, help the spiral stirring leaf to carry out quick stirring.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic cross-sectional view of the mixing drum according to the present invention;

FIG. 3 is a schematic cross-sectional view of the motor case of the present invention;

fig. 4 is an enlarged schematic view of the structure at a in fig. 3 according to the present invention.

In the figure: 1. a base; 2. a support block; 3. a mixing drum; 4. a liquid inlet pipe; 5. a medicine injection tube; 6. a liquid outlet pipe; 7. a filter assembly; 701. a filter cartridge; 702. a filtration pore; 703. a sinking-assisting block; 704. a first connecting plate; 705. an auxiliary block; 706. a through groove; 8. a lifting assembly; 801. a lifting motor; 802. a transmission screw rod; 803. moving the tube; 804. a limiting block; 805. a second connecting plate; 806. a support bar; 807. an auxiliary plate; 808. a limiting plate; 809. a threaded hole; 810. a screw; 811. a power-assisted disc; 9. a stirring motor; 10. a transmission rod; 11. a spiral stirring blade; 12. a motor case; 13. installing a box; 14. a baffle plate; 15. a limiting groove; 16. a stabilizing disc; 17. a connecting rod; 18. a first electrically operated valve; 19. a second electrically operated valve; 20. a third electrically operated valve; 21. a switch panel; 22. and a through hole.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1:

referring to fig. 1-4, the utility model provides a stirring device for preparing protease K, which comprises a base 1, wherein a supporting block 2 is fixedly arranged at the top end of the base 1, a stirring cylinder 3 is fixedly arranged at the top end of the supporting block 2, a stirring motor 9 is fixedly arranged at one end of the stirring cylinder 3, a transmission rod 10 is fixedly arranged on a transmission shaft of the stirring motor 9, a spiral stirring blade 11 is fixedly arranged on the surface of the transmission rod 10, an installation box 13 is fixedly arranged at the other end of the stirring cylinder 3, a filtering assembly 7 is arranged inside the installation box 13, a motor box 12 is fixedly arranged at the bottom end of the installation box 13, and a lifting assembly 8 is arranged at the top end of the motor box 12;

a baffle plate 14 is fixedly arranged on one side of the top end of the motor case 12, and a limiting groove 15 which slides with the limiting block 804 is formed in one side of the baffle plate 14;

a liquid inlet pipe 4, a medicine injection pipe 5 and a liquid outlet pipe 6 are respectively fixedly arranged on the surface of the mixing drum 3, a first electric valve 18 is fixedly arranged on the surface of the liquid inlet pipe 4, a second electric valve 19 is fixedly arranged on the surface of the medicine injection pipe 5, and a third electric valve 20 is fixedly arranged on the surface of the liquid outlet pipe 6;

a stabilizing disc 16 is arranged inside the mixing drum 3, a plurality of connecting rods 17 are fixedly arranged on the surface of the stabilizing disc 16, one ends of the connecting rods 17 are fixedly arranged on the inner wall of the mixing drum 3, the stabilizing disc 16 is rotatably connected with the transmission rod 10 through a sealing bearing, a through hole 22 is formed in one side of the mounting box 13, and the mounting box 13 is communicated with the inside of the mixing drum 3 through the through hole 22;

the fixed flush mounting plate of switch panel 21 that is equipped with in the front of supporting shoe 2, the surface of flush mounting plate 21 is equipped with agitator motor switch, first electric valve switch, second electric valve switch and third electric valve switch respectively, agitator motor 9, first electric valve 18, second electric valve 19 and third electric valve 20 respectively through agitator motor switch, first electric valve switch, second electric valve switch and third electric valve switch and external power source electric connection.

Example 2:

the filtering component 7 comprises a filtering box 701, the filtering box 701 is arranged at the bottom inside the installation box 13 in a sliding manner, a plurality of filtering holes 702 are formed in the bottom of the filtering box 701, a sinking assisting block 703 and a first connecting plate 704 are respectively fixedly arranged at the top of the filtering box 701, the surface of the sinking assisting block 703 and the surface of the first connecting plate 704 are both connected with the inner wall of the installation box 13 in a sliding manner, an auxiliary block 705 is fixedly arranged at the top end of the first connecting plate 704, and a through groove 706 is formed in the middle of the auxiliary block 705;

specifically, as shown in fig. 1, 3, and 4, the filter box 701 is first placed inside the installation box 13, the filter box 701 is supported by the inner wall at the bottom end of the installation box 13, the sediment is introduced into the installation box 13 by the spiral stirring blade 11 and then falls into the inside of the filter box 701 through the inclined plane of the settling block 703, when the sediment needs to be taken out, the filter box 701 is driven by the lifting assembly 8 to slide out of the installation box 13, the liquid falls into the installation box 13 through the filter hole 702, and can participate in next stirring and adjustment, thereby avoiding waste, the sediment is taken out of the installation box 13 by the filter box 701, the auxiliary block 705 is taken from the auxiliary plate 807, the filter box 701 is taken down, and the sediment is cleaned.

Example 3:

the lifting assembly 8 comprises a lifting motor 801, the lifting motor 801 is a forward and reverse rotation motor, the lifting motor 801 is fixedly arranged on the inner wall of the top end of the motor box 12, a transmission screw 802 is fixedly arranged on a transmission shaft of the lifting motor 801, the surface of the transmission screw 802 is connected with a moving pipe 803 through a screw nut and a thread, the surface of the moving pipe 803 is respectively fixedly provided with a limiting block 804 and two second connecting plates 805, the top ends of the two second connecting plates 805 are respectively fixedly provided with a supporting rod 806, the top ends of the two supporting rods 806 are fixedly provided with an auxiliary plate 807 which slides relative to the through groove 706, the top end of the auxiliary plate 807 is fixedly provided with a limiting plate 808, one side of the limiting plate 808 is slidably connected with one side of the auxiliary block 705, the surface of the auxiliary plate 807 is provided with a threaded hole 809, the inner wall of the threaded hole 809 is connected with a screw 810, and the top end of the screw 810 is fixedly provided with a power-assisting disc 811;

a lifting motor switch is arranged on the surface of the switch panel 21, and the lifting motor 801 is electrically connected with an external power supply through the lifting motor switch;

specifically, as shown in fig. 1, 3, and 4, the lifting motor 801 is first started by the lifting motor switch, the lifting motor 801 drives the transmission screw rod 802 to rotate, the transmission screw rod 802 rotates to drive the moving pipe 803 to move upward, and further drives the support rod 806 to move upward, the support rod 806 drives the auxiliary plate 807 to move upward, when the auxiliary plate 807 needs to move downward, the lifting motor 801 is reversely rotated by the lifting motor switch, and the moving pipe 803 can be driven to move downward, so that the auxiliary plate 807 moves downward, the position of the auxiliary block 705 is conveniently limited by the limit plate 808, and the position of the first connection plate 704 is further limited, so that the filter cartridge 701 can slide into the installation box 13, the screw 810 prevents the auxiliary block 705 from sliding down when the auxiliary plate 807 moves up and down, and the safety is enhanced.

The working principle is as follows: when the stirring device is used in detail, firstly, a stirring motor switch starts a stirring motor 9, the stirring motor 9 rotates to drive a transmission rod 10 to rotate, the transmission rod 10 is rotationally connected with a stabilizing disc 16 to further enhance the structural stability, then, the stirring motor 9 is operated to rapidly rotate, the transmission rod 10 drives a spiral stirring blade 11 to rotate, a first electric valve 18 is started through the first electric valve switch, filtrate is introduced, a second electric valve 19 is started through the second electric valve switch, a proper amount of ammonium sulfate solution is added, the spiral stirring blade 11 is continuously stirred, meanwhile, the generated precipitate is pushed into the installation box 13, the filter box 701 is propped against the inner wall at the bottom end of the installation box 13, the precipitate falls into the filter box 701 through the inclined plane of an auxiliary precipitation block 703, a dilute hydrochloric acid solution is added through an opening at the top end of the installation box 13 to adjust the pH value of suspension, liquid is taken out through an opening at the top end of the installation box 13 so as to determine the pH value of suspension, when sediment needs to be taken out, the lifting motor 801 is started through a lifting motor switch, the lifting motor 801 drives the transmission screw rod 802 to rotate, the transmission screw rod 802 rotates to enable the moving pipe 803 to move upwards and further drive the supporting rod 806 to move upwards, the supporting rod 806 drives the auxiliary plate 807 to move upwards, the auxiliary plate 807 drives the filter box 701 to move upwards, the filter box 701 slides out of the installation box 13, liquid falls into the installation box 13 through the filter hole 702 and can participate in next stirring and adjustment, waste is avoided, the sediment is taken out of the installation box 13 by the filter box 701, the auxiliary force disc 811 rotates, the screw rod 810 is enabled to be separated from the threaded hole 809, the auxiliary block 705 slides out along the auxiliary plate 807, the filter box 701 is further taken down, and sediment is cleaned.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a agitating unit is used in protease K preparation, includes base (1), its characterized in that, the fixed supporting shoe (2) that is equipped with in top of base (1), the fixed churn (3) that is equipped with in top of supporting shoe (2), the fixed agitator motor (9) that is equipped with in one end of churn (3), the fixed transfer line (10) that is equipped with of transmission shaft of agitator motor (9), the fixed surface of transfer line (10) is equipped with spiral stirring leaf (11), the other end of churn (3) is fixed and is equipped with install bin (13), the inside of install bin (13) is equipped with filter assembly (7), the bottom mounting of install bin (13) is equipped with motor case (12), the top of motor case (12) is equipped with lifting unit (8).

2. The stirring device for preparing proteinase K according to claim 1, wherein the stirring device comprises: the filter assembly (7) comprises a filter box (701), the filter box (701) is arranged at the bottom of the mounting box (13) in a sliding mode, a plurality of filter holes (702) are formed in the bottom of the filter box (701), a sinking assisting block (703) and a first connecting plate (704) are fixedly arranged at the top of the filter box (701) respectively, the surface of the sinking assisting block (703) and the surface of the first connecting plate (704) are connected with the inner wall of the mounting box (13) in a sliding mode, an auxiliary block (705) is fixedly arranged at the top end of the first connecting plate (704), and a through groove (706) is formed in the middle of the auxiliary block (705).

3. The stirring device for preparing proteinase K according to claim 1, wherein the stirring device comprises: the lifting assembly (8) comprises a lifting motor (801), the lifting motor (801) is a forward and reverse rotation motor, the lifting motor (801) is fixedly arranged on the inner wall of the top end of the motor box (12), a transmission screw (802) is fixedly arranged on a transmission shaft of the lifting motor (801), a moving pipe (803) is connected to the surface of the transmission screw (802) through screw nuts and threads, a limiting block (804) and two second connecting plates (805) are respectively fixedly arranged on the surface of the moving pipe (803), supporting rods (806) are respectively fixedly arranged at the top ends of the two second connecting plates (805), auxiliary plates (807) sliding with the through grooves (706) are fixedly arranged at the top ends of the two supporting rods (806), a limiting plate (808) is fixedly arranged at the top end of the auxiliary plate (807), and one side of the limiting plate (808) is slidably connected with one side of the auxiliary block (705), threaded holes (809) are formed in the surface of the auxiliary plate (807), a screw rod (810) is connected to the inner wall of each threaded hole (809) in a threaded mode, and a power assisting disc (811) is fixedly arranged at the top end of each screw rod (810).

4. The stirring device for preparing proteinase K according to claim 1, wherein the stirring device comprises: a baffle (14) is fixedly arranged on one side of the top end of the motor box (12), and a limiting groove (15) which slides with the limiting block (804) is formed in one side of the baffle (14).

5. The stirring device for producing proteinase K according to claim 3, wherein the stirring device comprises: the surface of churn (3) is fixed respectively and is equipped with feed liquor pipe (4), injection pipe (5) and drain pipe (6), the fixed surface of feed liquor pipe (4) is equipped with first motorised valve (18), the fixed surface of injection pipe (5) is equipped with second motorised valve (19), the fixed surface of drain pipe (6) is equipped with third motorised valve (20).

6. The stirring device for preparing proteinase K according to claim 1, wherein the stirring device comprises: the inside of churn (3) is equipped with stable dish (16), the fixed surface of stable dish (16) is equipped with a plurality of connecting rod (17), a plurality of the inner wall of the equal fixed setting in churn (3) of one end of connecting rod (17), stable dish (16) are rotated through sealed bearing and transfer line (10) and are connected, through-hole (22) have been seted up to one side of install bin (13), install bin (13) are linked together through the inside of through-hole (22) with churn (3).

7. The stirring apparatus for producing proteinase K according to claim 5, wherein the stirring apparatus further comprises: the fixed flush mounting plate of being equipped with of front of supporting shoe (2) (21), the surface of flush mounting plate (21) is equipped with agitator motor switch, first electric valve switch, second electric valve switch, third electric valve switch and driving motor switch respectively, agitator motor (9), first electric valve (18), second electric valve (19), third electric valve (20) and elevator motor (801) are respectively through agitator motor switch, first electric valve switch, second electric valve switch, third electric valve switch and driving motor switch and external power source electric connection.