CN216571655U - Exosome filters extraction element - Google Patents
Exosome filters extraction element Download PDFInfo
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- CN216571655U CN216571655U CN202122422590.5U CN202122422590U CN216571655U CN 216571655 U CN216571655 U CN 216571655U CN 202122422590 U CN202122422590 U CN 202122422590U CN 216571655 U CN216571655 U CN 216571655U
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- fixedly connected
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- exosome
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- 238000000605 extraction Methods 0.000 title claims abstract description 58
- 210000001808 exosome Anatomy 0.000 title claims abstract description 38
- 238000001914 filtration Methods 0.000 claims abstract description 31
- 238000005070 sampling Methods 0.000 claims abstract description 22
- 239000011521 glass Substances 0.000 claims description 3
- 239000000463 material Substances 0.000 claims 1
- 239000007788 liquid Substances 0.000 description 15
- 230000000694 effects Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
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Abstract
The utility model provides an exosome filtering and extracting device, which relates to the technical field of exosomes and aims to solve the problems that when exosomes are extracted through a test tube, air in the test tube is inconvenient to empty, and the exosomes are easy to contact with the air in the test tube, so that the quality of the exosomes is influenced; the extraction device consists of a fixed structure, a rotating structure, a culture device, a filtering device and a sampling device; the upper part of the fixed structure is movably connected with a rotating structure; the rotating structure is respectively movably connected with the filtering device and the sampling device; the culture device is movably connected to the rotating structure; and the filtering device is arranged at the bottom of the sampling device. According to the sampling device, the control rod slides downwards, so that air in the extraction pipe can be discharged, and air in the pipe head on the extraction pipe can be simultaneously discharged through the plug-in component, so that the phenomenon that air remains in the extraction pipe and influences the quality of the collected exosomes is avoided.
Description
Technical Field
The utility model relates to the technical field of exosomes, in particular to an exosome filtering and extracting device.
Background
All cultured cell types secrete exosomes and exosomes are naturally present in body fluids, including blood, saliva, urine, cerebrospinal fluid and milk. Regarding their secretion and uptake and their composition, the engineering of exosome extraction requires staff to perform the extraction in a specific way.
The inventor finds that when the existing exosome filtering and extracting device is used, most of the existing exosome filtering and extracting device adopts the test tube to extract, but when the existing exosome filtering and extracting device extracts through the test tube, air in the test tube is not convenient to empty, and exosomes are easy to contact with the air in the test tube, so that the exosome quality is influenced.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide an exosome filtering and extracting device, which solves the problems that when exosomes are extracted through a test tube in the background technology, air in the test tube is inconvenient to empty, and exosomes are easy to contact with the air in the test tube, so that the quality of the exosomes is influenced.
The purpose and the efficacy of the exosome filtering and extracting device are achieved by the following specific technical means:
an exosome filtering and extracting device comprises a fixed structure, a rotating structure, a culture device, a filtering device and a sampling device;
the upper part of the fixed structure is movably connected with a rotating structure;
the rotating structure is respectively and movably connected with the filtering device and the sampling device;
the culture device is movably connected to the rotating structure;
and the filtering device is arranged at the bottom of the sampling device.
Further, the fixing structure includes:
the supporting rod is fixedly connected above the bottom plate;
the supporting rod is fixedly connected with the connecting plate;
the connecting plate, the inside of connecting plate is equipped with the fretwork groove, still is equipped with the cartridge groove on the connecting plate.
Further, the rotation structure includes:
the rotary table is fixedly connected to the connecting shaft;
the connecting shaft is fixedly connected with the bearing, and a fixing plate is fixedly connected to the connecting shaft;
the fixing plates are arranged in two groups;
the bearing is fixedly connected with the connecting plate through the hollow groove;
preformed hole, preformed hole are three sets of settings, and three sets of preformed holes set up respectively on the carousel, and the inside swing joint of preformed hole has the extraction pipe.
Furthermore, the culture device is made of transparent glass and is connected with the connecting plate in a plug-in manner through a plug-in groove.
Further, the filtering apparatus includes:
one end of the fixing frame is fixedly connected with the connecting piece, and the bottom of the fixing frame is fixedly connected with the lapping piece A;
the connecting piece is arranged between the two groups of fixing plates and is used for rotationally connecting the connecting piece to the connecting shaft, the connecting piece can be limited by the fixing plates at the same time, and when the fixing frame is rotated, the filter screen can be driven to rotate, the filter screen is placed below the extraction tube, collected liquid is filtered, and exosomes are extracted;
the filter screen, the filter screen passes through overlap joint spare A overlap joint and on the mount, and the filter screen is three sets of settings, and its effect does, can set up the filter screen in the below of three sets of extraction pipes simultaneously, filters.
Further, sampling device is three groups of settings, and three groups of sampling device include respectively:
the outer side of the extraction tube is fixedly connected with a lapping piece B, the extraction tube is lapped with the turntable through the lapping piece B, and the bottom end of the extraction tube is fixedly connected with a tube head;
the baffle is fixedly connected inside the extraction tube and is in sliding connection with the control rod;
one end of the control rod is fixedly connected with a sliding plate, and an elastic part is movably connected on the control rod;
the sliding plate is connected inside the extraction tube in a sliding mode, the bottom of the sliding plate is fixedly connected with the plug-in component, and the sliding plate is arranged below the baffle plate and used for simultaneously discharging air in tube heads on the extraction tube through the plug-in component, so that the phenomenon that the air is remained inside the extraction tube and the quality of the collected exosomes is influenced is avoided;
the plug-in component is connected with the pipe head in a plug-in manner;
elastic component, the both ends of elastic component respectively with baffle, control lever fixed connection, elastic component adopts the spring, and its effect does, through the control lever that slides downwards, can be with extracting the inside air escape, loosens the control lever after the discharge, and the control lever is withdrawed automatically through the elastic component, can gather and draw liquid when withdrawing, and liquid can't flow out simultaneously, when having avoided drawing through the test tube, needs the staff to extrude the rubber ball for a long time.
Compared with the prior structure, the utility model has the following advantages:
according to the filter device, the connecting piece is rotatably connected to the connecting shaft and can be limited by the fixing plate, when the fixing frame is rotated, the filter screen can be driven to rotate, the filter screen is placed below the extraction tube, collected liquid is filtered, and exosomes are extracted.
According to the sampling device, the control rod slides downwards, so that air in the extraction tube can be discharged, the control rod is loosened after the air is discharged, the control rod is automatically retracted through the elastic piece, liquid can be collected and extracted during retraction, meanwhile, the liquid cannot flow out, the phenomenon that a worker needs to extrude a rubber ball for a long time during extraction through a test tube is avoided, meanwhile, air in a tube head on the extraction tube can be discharged simultaneously through the insertion piece, and the phenomenon that air remains in the extraction tube and influences the quality of an exosome after collection is avoided.
Drawings
FIG. 1 is a schematic top view of the present invention;
FIG. 2 is a schematic structural view of a fixing structure according to the present invention;
FIG. 3 is a schematic view of the structure of a culture apparatus according to the present invention;
FIG. 4 is a schematic structural view of a rotary structure of the present invention;
FIG. 5 is a schematic view of the structure of the filter device of the present invention;
fig. 6 is a schematic structural diagram of the sampling device of the present invention.
In the figure:
1. an exosome filtration and extraction device;
2. a fixed structure; 201. a base plate; 202. a support bar; 203. a connecting plate; 204. hollowing out the grooves; 205. inserting the slots;
3. a rotating structure; 301. a turntable; 302. reserving a hole; 303. a bearing; 304. a connecting shaft; 305. a fixing plate;
4. a culture device;
5. a filtration device; 501. a fixed mount; 502. a connecting member; 503. connecting grooves; 504. a lap joint A; 505. filtering with a screen;
6. a sampling device; 601. an extraction tube; 602. a lap joint B; 603. a pipe head; 604. a baffle plate; 605. a sliding plate; 606. A cartridge; 607. a control lever; 608. an elastic member.
Detailed Description
Reference will now be made in detail to the embodiments of the present invention, examples of which are illustrated in the accompanying drawings and described below. While the utility model will be described in conjunction with the exemplary embodiments, it will be understood that the description is not intended to limit the utility model to the exemplary embodiments. On the contrary, the utility model is intended to cover not only the exemplary embodiments, but also various alternative, modified, equivalent and other embodiments, which may be included within the spirit and scope of the utility model as defined by the appended claims.
Referring to fig. 1 to 6, an exosome filtration-extraction device 1 comprises a fixed structure 2, a rotating structure 3, a culture device 4, a filtration device 5 and a sampling device 6; the upper part of the fixed structure 2 is movably connected with a rotating structure 3; the rotating structure 3 is movably connected with the filtering device 5 and the sampling device 6 respectively; the culture device 4 is movably connected to the rotating structure 3; and the filtering device 5 is arranged at the bottom of the sampling device 6.
As shown in fig. 2, wherein the fixing structure 2 comprises: a support rod 202 is fixedly connected above the bottom plate 201; the supporting rod 202, the supporting rod 202 is fixedly connected with the connecting plate 203; the connecting plate 203 is provided with a hollow groove 204 inside, and the connecting plate 203 is also provided with an inserting groove 205.
As shown in fig. 4, wherein the rotating structure 3 includes: the turntable 301, the turntable 301 is fixedly connected to the connecting shaft 304; the connecting shaft 304 is fixedly connected with the bearing 303, and a fixing plate 305 is fixedly connected to the connecting shaft 304; the fixing plates 305, the fixing plates 305 are arranged in two groups; the bearing 303, the bearing 303 is fixedly connected with the connecting plate 203 through the hollow groove 204; preformed hole 302, preformed hole 302 are three sets of settings, and three sets of preformed holes 302 set up respectively on carousel 301, and the inside swing joint of preformed hole 302 has extraction pipe 601.
As shown in FIG. 3, the culture apparatus 4 is made of transparent glass, and the culture apparatus 4 is inserted into and connected to the connection plate 203 through the insertion groove 205.
As shown in fig. 5, wherein the filtering device 5 comprises: one end of the fixing frame 501 is fixedly connected with the connecting piece 502, and the bottom of the fixing frame 501 is fixedly connected with a lap joint piece A504; a connecting piece 502, a connecting groove 503 is arranged inside the connecting piece 502, the connecting piece 502 is rotatably connected with the connecting shaft 304 through the connecting groove 503, and the connecting piece 502 is arranged between the two sets of fixing plates 305; filter screen 505, filter screen 505 pass through bridging member A504 overlap joint on mount 501, and connecting piece 502 rotates to be connected on connecting axle 304, and connecting piece 502 can carry on spacingly through fixed plate 305 simultaneously, when rotating mount 501, can drive filter screen 505 and rotate, places filter screen 505 in the below of extraction pipe 601, filters the liquid of collection to draw the exosome.
As shown in fig. 6, the sampling devices 6 are arranged in three groups, and the three groups of sampling devices 6 respectively include: the outer side of the extraction pipe 601 is fixedly connected with a lapping part B602, the extraction pipe 601 is lapped with the turntable 301 through the lapping part B602, and the bottom end of the extraction pipe 601 is fixedly connected with a pipe head 603; the baffle 604 is fixedly connected inside the extraction pipe 601, and the baffle 604 is connected with the control rod 607 in a sliding manner; a control lever 607, one end of the control lever 607 is fixedly connected with a sliding plate 605, and the control lever 607 is movably connected with an elastic member 608; the sliding plate 605 is connected inside the extraction pipe 601 in a sliding manner, the bottom of the sliding plate 605 is fixedly connected with the cartridge 606, and the sliding plate 605 is arranged below the baffle 604; the cartridge 606 is connected with the pipe head 603 in a cartridge mode; elastic component 608, elastic component 608's both ends respectively with baffle 604, control lever 607 fixed connection, through the control lever 607 that slides down, can be with the inside air escape of extraction tube 601, loosen control lever 607 after the discharge, control lever 607 withdraws automatically through elastic component 608, can gather and draw liquid when withdrawing, liquid can't flow out simultaneously, when having avoided drawing through the test tube, need the staff to extrude the rubber ball for a long time, simultaneously can discharge the air in the tube head 603 on the extraction tube 601 simultaneously through cartridge 606, avoided the air to remain in the inside of extraction tube 601, influence the quality after the exosome is collected.
In another embodiment, the control lever 607 is a screw, and the control lever 607 is rotatably connected to the baffle 604, when the control lever 607 is rotated, the sliding plate 605 can be automatically moved downwards to discharge the air in the extraction pipe 601, and after the air is discharged, the control lever 607 can be rotated to drive the sliding plate 605 to lift up to collect the liquid, and the sliding plate 605 can be conveniently fixed by the control lever 607.
The working principle of the embodiment is as follows:
in the novel implementation, liquid can be collected through the extraction tube 601, when the extraction tube 601 is used, the control rod 607 is pressed downwards to discharge air in the extraction tube 601, the control rod 607 is loosened after the air is discharged, the control rod 607 is automatically retracted through the elastic piece 608, the liquid can be collected and extracted when the liquid is retracted, the inserting piece 606 can simultaneously discharge air in the pipe head 603 on the extraction tube 601, air is prevented from remaining in the extraction tube 601, the extraction tube 601 is placed on the rotary disc 301 through the overlapping piece B602 after the extraction is completed, the connecting plate 203 is arranged below the rotary disc 301, the culture device 4 is movably connected to the connecting plate 203, the liquid is injected into the culture device 4 through the sliding control rod 607, the filter screen 505 is arranged below the extraction tube 601, when the fixing frame 501 is rotated, the filter screen 505 can be driven to rotate, the filter screen 505 is placed below the extraction tube 601, the collected liquid was filtered.
The utility model is not described in detail, but is well known to those skilled in the art.
In summary, although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the technical solutions described in the foregoing embodiments or equivalent substitutions for some technical features, and any modifications, equivalents, improvements, etc. made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (6)
1. The utility model provides an exosome filters extraction element which characterized in that: comprises a fixed structure (2), a rotating structure (3), a culture device (4), a filtering device (5) and a sampling device (6);
the upper part of the fixed structure (2) is movably connected with a rotating structure (3);
the rotating structure (3), the rotating structure (3) is respectively and movably connected with the filtering device (5) and the sampling device (6);
the culture device (4), the culture device (4) is movably connected to the rotating structure (3);
and the filtering device (5) is arranged at the bottom of the sampling device (6).
2. Exosome filtration extraction device according to claim 1, characterized in that: the fixed structure (2) comprises:
the supporting rod (202) is fixedly connected above the bottom plate (201);
the supporting rod (202), the supporting rod (202) is fixedly connected with the connecting plate (203);
the connecting plate (203), the inside of connecting plate (203) is equipped with fretwork groove (204), still is equipped with cartridge groove (205) on connecting plate (203).
3. Exosome filtration extraction device according to claim 1, characterized in that: the rotating structure (3) comprises:
the turntable (301), the turntable (301) is fixedly connected to the connecting shaft (304);
the connecting shaft (304), the connecting shaft (304) is fixedly connected with the bearing (303), and the connecting shaft (304) is fixedly connected with a fixing plate (305);
the fixing plates (305), the fixing plates (305) are arranged in two groups;
the bearing (303), the bearing (303) is fixedly connected with the connecting plate (203) through the hollow groove (204);
preformed hole (302), preformed hole (302) are three sets of settings, and three sets of preformed holes (302) set up respectively on carousel (301), and the inside swing joint of preformed hole (302) has extraction pipe (601).
4. Exosome filtration extraction device according to claim 1, characterized in that: the culture device (4) is made of transparent glass materials, and the culture device (4) is connected with the connecting plate (203) in a plug-in mode through the plug-in groove (205).
5. Exosome filtration extraction device according to claim 1, characterized in that: the filtering device (5) comprises:
one end of the fixing frame (501) is fixedly connected with the connecting piece (502), and the bottom of the fixing frame (501) is fixedly connected with a lap joint piece A (504);
the connecting piece (502), the inside of the connecting piece (502) is provided with a connecting groove (503), the connecting piece (502) is rotatably connected with the connecting shaft (304) through the connecting groove (503), and the connecting piece (502) is arranged between the two groups of fixing plates (305);
the filter screen (505), the filter screen (505) passes through bridging A (504) lap joint and connects on mount (501).
6. Exosome filtration extraction device according to claim 1, characterized in that: sampling device (6) are three groups of settings, and three groups of sampling device (6) include respectively:
the outer side of the extraction pipe (601) is fixedly connected with a lapping piece B (602), the extraction pipe (601) is lapped with the turntable (301) through the lapping piece B (602), and the bottom end of the extraction pipe (601) is fixedly connected with a pipe head (603);
the baffle (604), the baffle (604) is fixedly connected to the inside of the extraction pipe (601), and the baffle (604) is connected with the control rod (607) in a sliding manner;
one end of the control rod (607) is fixedly connected with a sliding plate (605), and the control rod (607) is movably connected with an elastic piece (608);
the sliding plate (605) is connected inside the extraction pipe (601) in a sliding manner, the bottom of the sliding plate (605) is fixedly connected with a plug-in component (606), and the sliding plate (605) is arranged below the baffle (604);
the cartridge (606), the cartridge (606) is connected with the pipe head (603) in a cartridge manner;
the two ends of the elastic piece (608) are respectively fixedly connected with the baffle (604) and the control rod (607).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122422590.5U CN216571655U (en) | 2021-10-08 | 2021-10-08 | Exosome filters extraction element |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122422590.5U CN216571655U (en) | 2021-10-08 | 2021-10-08 | Exosome filters extraction element |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216571655U true CN216571655U (en) | 2022-05-24 |
Family
ID=81640055
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122422590.5U Expired - Fee Related CN216571655U (en) | 2021-10-08 | 2021-10-08 | Exosome filters extraction element |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216571655U (en) |
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2021
- 2021-10-08 CN CN202122422590.5U patent/CN216571655U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20220524 |