CN216560438U - Liquid adding device for protein precipitation reaction - Google Patents

Liquid adding device for protein precipitation reaction Download PDF

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Publication number
CN216560438U
CN216560438U CN202122622864.5U CN202122622864U CN216560438U CN 216560438 U CN216560438 U CN 216560438U CN 202122622864 U CN202122622864 U CN 202122622864U CN 216560438 U CN216560438 U CN 216560438U
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protein
storage chamber
stirring
protein liquid
precipitant
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CN202122622864.5U
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Chinese (zh)
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占远
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Suzhou Fangda New Drug Development Co ltd
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Suzhou Fangda New Drug Development Co ltd
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Abstract

The utility model provides a liquid adding device for protein precipitation reaction, which belongs to the technical field of protein processing and comprises a box body, a stirring part, a driving part and a pumping part, wherein a partition plate is fixedly arranged in the box body, a protein liquid storage chamber is arranged on one side of the partition plate, a precipitant storage chamber is arranged on the other side of the partition plate, a liquid conveying pipe is arranged on one side of the partition plate, which faces the protein liquid storage chamber, the liquid conveying pipe is communicated with the precipitant storage chamber, two groups of one-way valves are arranged on the liquid conveying pipe, an extension cylinder communicated with the liquid conveying pipe is arranged between the two groups of one-way valves, the pumping part is arranged in the extension cylinder, the stirring part is arranged in the protein liquid storage chamber, and the driving part is used for driving the stirring part to rotate. Compared with the prior art, the embodiment of the utility model can realize the efficient mixing of the precipitating agent and the protein liquid, and has the advantages of convenient use and high reaction efficiency of the protein liquid and the precipitating agent.

Description

Liquid adding device for protein precipitation reaction
Technical Field
The utility model belongs to the technical field of protein processing, and particularly relates to a liquid adding device for protein precipitation reaction.
Background
At present, in the protein processing process, the protein liquid needs to be subjected to precipitation treatment, so that the protein in the protein liquid is deposited at the bottom of a container and separated from the protein stock solution, and a precipitating agent is usually added in the precipitation process to accelerate the deposition of the protein.
In the prior art, the precipitating agent is mostly injected from the upper part of the protein liquid, so that the added protein liquid can not be quickly mixed with the protein liquid which is positioned in a sinking state in a short time, the precipitating agent can not quickly react with all the protein liquid, and the precipitation efficiency of the protein is low.
Disclosure of Invention
Aiming at the defects of the prior art, the technical problem to be solved by the embodiment of the utility model is to provide a liquid adding device for protein precipitation reaction.
In order to solve the technical problems, the utility model provides the following technical scheme:
a liquid adding device for protein precipitation reaction, which comprises a box body, a stirring part, a driving part and a pumping part,
a clapboard is fixedly arranged in the box body, one side of the clapboard is provided with a protein liquid storage chamber, the other side is provided with a precipitant storage chamber,
an infusion tube is arranged on one side of the partition board facing the protein liquid storage chamber and is communicated with the precipitant storage chamber, two groups of one-way valves are arranged on the infusion tube, an extension tube communicated with the infusion tube is arranged between the two groups of one-way valves,
the pumping part is arranged in the extension cylinder, the stirring part is arranged in the protein liquid storage chamber,
the drive division is used for driving stirring portion rotates to stir protein liquid, drive division drive stirring pivoted in can drive pump portion along extending the inside reciprocating motion of a section of thick bamboo, inside with the inside precipitant pump of precipitant apotheca to protein liquid apotheca.
As a further improvement of the utility model: the stirring part comprises a stirring drum and a plurality of stirring rods fixedly arranged on the side wall of the stirring drum,
the mixing drum is rotatably arranged in the protein liquid storage chamber, the inner wall of the mixing drum is provided with a curve groove,
the drive division includes the actuating lever and fixed the setting is in the extension rod of actuating lever lateral wall, actuating lever one end extends to inside the churn, the other end extends to the box is outside, the extension rod extends to inside and with curve groove sliding fit of curve groove.
As a further improvement of the utility model: the curve grooves are distributed on the inner wall of the stirring cylinder in a spiral structure.
As a further improvement of the utility model: and a handle is fixedly arranged at one end of the driving rod, which is positioned outside the box body.
As a still further improvement of the utility model: a supporting cylinder penetrates through the middle part of the clapboard and is fixedly arranged, the driving rod penetrates through the supporting cylinder and is movably matched with the supporting cylinder, one end of the extending cylinder far away from the infusion tube is communicated with the supporting cylinder,
the pumping part comprises a piston movably arranged in the extension cylinder, a first connecting rod fixedly arranged on one side of the piston facing the support cylinder and a second connecting rod hinged to one end, far away from the piston, of the first connecting rod, and the second connecting rod is hinged to the driving rod.
Compared with the prior art, the utility model has the beneficial effects that:
in the embodiment of the utility model, the stirring part is driven to rotate by the driving part so as to stir the protein liquid, meanwhile, the pumping part can be driven by the driving part to reciprocate in the extension cylinder, when the pumping part moves towards one direction, the precipitator in the precipitator storage chamber can be pumped into the infusion tube, when the pumping part moves towards the other direction, the precipitator entering the infusion tube can be extruded out from the end part of the infusion tube, so that the precipitator enters the protein liquid storage chamber to be mixed with the protein liquid, and the reaction speed of the protein liquid and the precipitator is accelerated by matching the stirring effect of the stirring part on the protein liquid.
Drawings
FIG. 1 is a schematic diagram of a liquid adding device for protein precipitation reaction;
FIG. 2 is a schematic diagram of the structure of the stirring part of the liquid adding device for protein precipitation reaction;
FIG. 3 is an enlarged view of area A of FIG. 1;
in the figure: 1-box body, 11-protein liquid storage chamber, 12-precipitant storage chamber, 2-stirring part, 21-stirring cylinder, 22-stirring rod, 23-curve groove, 3-driving part, 31-extension rod, 32-driving rod, 4-clapboard, 41-transfusion tube, 42-supporting cylinder, 43-extension cylinder, 5-pumping part, 51-piston, 52-first connecting rod, 53-second connecting rod.
Detailed Description
The technical solution of the present patent will be described in further detail with reference to the following embodiments.
Reference will now be made in detail to embodiments of the present patent, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present patent and are not to be construed as limiting the present patent.
Referring to fig. 1, the embodiment provides a liquid adding device for protein precipitation reaction, which includes a box 1, a stirring portion 2, a driving portion 3, and a pumping portion 5, a partition plate 4 is fixedly disposed inside the box 1, a protein liquid storage chamber 11 is disposed on one side of the partition plate 4, a precipitant storage chamber 12 is disposed on the other side of the partition plate, a liquid transport tube 41 is disposed on one side of the partition plate 4 facing the protein liquid storage chamber 11, the liquid transport tube 41 is communicated with the precipitant storage chamber 12, two sets of one-way valves are mounted on the liquid transport tube 41, an extension tube 43 communicated with the liquid transport tube 41 is disposed between the two sets of one-way valves, the pumping portion 5 is disposed inside the extension tube 43, the stirring portion 2 is disposed inside the protein liquid storage chamber 11, the driving portion 3 is configured to drive the stirring portion 2 to rotate so as to stir protein liquid, the driving portion 3 drives the stirring portion 2 to rotate and simultaneously drives the pumping portion 5 to reciprocate along the interior of the extension tube 43, to pump the precipitant inside the precipitant storage chamber 12 to the inside of the protein solution storage chamber 11.
Drive stirring portion 2 through drive division 3 and rotate, with stir the processing to protein liquid, drive division 3 still can drive pumping portion 5 and extend a 43 inside reciprocating motion, pumping portion 5 is when removing to a direction, can be with inside precipitant suction to transfer line 41 of precipitant apotheca 12 inside, pumping portion 5 can be with getting into 41 inside precipitant to transfer line 41 tip extrusion of transfer line when removing to another direction, make the precipitant mix with protein liquid in getting into protein liquid apotheca 11, cooperation stirring portion 2 is to the stirring effect of protein liquid, with the reaction rate of accelerating protein liquid and precipitant, thereby improve the precipitation efficiency of protein.
Referring to fig. 1, in an embodiment, the stirring portion 2 includes a stirring drum 21 and a plurality of stirring rods 22 fixedly disposed on a side wall of the stirring drum 21, the stirring drum 21 is rotatably mounted inside the protein liquid storage chamber 11, a curved groove 23 is disposed on an inner wall of the stirring drum 21, the driving portion 3 includes a driving rod 32 and an extension rod 31 fixedly disposed on a side wall of the driving rod 32, one end of the driving rod 32 extends into the stirring drum 21, the other end extends to the outside of the box body 1, and the extension rod 31 extends into the curved groove 23 and is in sliding fit with the curved groove 23.
Through reciprocal push-and-pull actuating lever 32 for actuating lever 32 utilizes the cooperation of extension rod 31 and curve groove 23 along the inside reciprocating motion of churn 21, in order to drive churn 21 reciprocating rotation, and then drives puddler 22 reciprocating rotation, utilizes puddler 22 to stir protein liquid.
In one embodiment, the curved grooves 23 are distributed in a spiral structure on the inner wall of the mixing drum 21.
Referring to fig. 1, in an embodiment, a handle is fixedly disposed at an end of the driving rod 32 located outside the box body 1, so as to facilitate the pushing and pulling operation of the driving rod 32.
Referring to fig. 1, in an embodiment, a supporting cylinder 42 is fixedly disposed in the middle of the partition 4, the driving rod 32 penetrates through the supporting cylinder 42 and is movably engaged with the supporting cylinder 42, one end of the extending cylinder 43, which is away from the infusion tube 41, is communicated with the supporting cylinder 42, the pumping unit 5 includes a piston 51 movably disposed in the extending cylinder 43, a first connecting rod 52 fixedly disposed on one side of the piston 51, which faces the supporting cylinder 42, and a second connecting rod 53 hinged to one end of the first connecting rod 52, which is away from the piston 51, and one end of the second connecting rod 53, which is away from the first connecting rod 52, is hinged to the driving rod 32.
When reciprocal push-and-pull actuating lever 32, accessible second connecting rod 53 and the transmission effect between the first connecting rod 52, order about piston 51 along extending the inside reciprocating motion of a section of thick bamboo 43, the setting of two sets of check valves is coordinated, can be with inside the inside precipitant of precipitant apotheca 12 constantly by transfer line 41 pump to albumen liquid apotheca 11 inside, mix with the albumen liquid, combine the rotation effect of churn 21 and puddler 22 for the precipitant can fully contact with the albumen liquid, in order to improve reaction effect.
In the embodiment of the utility model, the stirring part 2 is driven to rotate by the driving part 3 to stir the protein liquid, meanwhile, the driving part 3 can drive the pumping part 5 to reciprocate in the extension cylinder 43, when the pumping part 5 moves towards one direction, the precipitator in the precipitator storage chamber 12 can be pumped into the infusion tube 41, when the pumping part 5 moves towards the other direction, the precipitator entering the infusion tube 41 can be extruded out from the end part of the infusion tube 41, so that the precipitator enters the protein liquid storage chamber 11 to be mixed with the protein liquid, and the stirring effect of the stirring part 2 on the protein liquid is matched to accelerate the reaction speed of the protein liquid and the precipitator.
Although the preferred embodiments of the present patent have been described in detail, the present patent is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present patent within the knowledge of those skilled in the art.

Claims (5)

1. A liquid adding device for protein precipitation reaction, which is characterized by comprising a box body, a stirring part, a driving part and a pumping part,
a clapboard is fixedly arranged in the box body, one side of the clapboard is provided with a protein liquid storage chamber, the other side is provided with a precipitant storage chamber,
an infusion tube is arranged on one side of the partition board facing the protein liquid storage chamber and is communicated with the precipitant storage chamber, two groups of one-way valves are arranged on the infusion tube, an extension tube communicated with the infusion tube is arranged between the two groups of one-way valves,
the pumping part is arranged in the extension cylinder, the stirring part is arranged in the protein liquid storage chamber,
the drive division is used for driving stirring portion rotates to stir protein liquid, drive division drive stirring pivoted in can drive pump portion along extending the inside reciprocating motion of a section of thick bamboo, inside with the inside precipitant pump of precipitant apotheca to protein liquid apotheca.
2. The protein precipitation reaction liquid adding device according to claim 1, wherein the stirring part comprises a stirring drum and a plurality of stirring rods fixedly arranged on the side wall of the stirring drum,
the mixing drum is rotatably arranged in the protein liquid storage chamber, the inner wall of the mixing drum is provided with a curve groove,
the drive division includes the actuating lever and fixed the setting is in the extension rod of actuating lever lateral wall, actuating lever one end extends to inside the churn, the other end extends to the box is outside, the extension rod extends to inside and with curve groove sliding fit of curve groove.
3. The device as claimed in claim 2, wherein the curved grooves are distributed in a spiral structure on the inner wall of the mixing drum.
4. The protein precipitation reaction liquid adding device as claimed in claim 2, wherein a handle is fixedly arranged at one end of the driving rod positioned outside the box body.
5. The protein precipitation reaction liquid adding device according to claim 2, wherein a support cylinder penetrates through and is fixedly arranged in the middle of the partition plate, the driving rod penetrates through the support cylinder and is movably matched with the support cylinder, one end of the extension cylinder, which is far away from the infusion tube, is communicated with the support cylinder,
the pumping part comprises a piston movably arranged in the extension cylinder, a first connecting rod fixedly arranged on one side of the piston facing the support cylinder and a second connecting rod hinged to one end, far away from the piston, of the first connecting rod, and the second connecting rod is hinged to the driving rod.
CN202122622864.5U 2021-10-29 2021-10-29 Liquid adding device for protein precipitation reaction Active CN216560438U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202122622864.5U CN216560438U (en) 2021-10-29 2021-10-29 Liquid adding device for protein precipitation reaction

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202122622864.5U CN216560438U (en) 2021-10-29 2021-10-29 Liquid adding device for protein precipitation reaction

Publications (1)

Publication Number Publication Date
CN216560438U true CN216560438U (en) 2022-05-17

Family

ID=81570303

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202122622864.5U Active CN216560438U (en) 2021-10-29 2021-10-29 Liquid adding device for protein precipitation reaction

Country Status (1)

Country Link
CN (1) CN216560438U (en)

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