CN216526803U - Automatic liquid injection and starting alarm clearing circuit based on flow cytometer - Google Patents
Automatic liquid injection and starting alarm clearing circuit based on flow cytometer Download PDFInfo
- Publication number
- CN216526803U CN216526803U CN202122738521.5U CN202122738521U CN216526803U CN 216526803 U CN216526803 U CN 216526803U CN 202122738521 U CN202122738521 U CN 202122738521U CN 216526803 U CN216526803 U CN 216526803U
- Authority
- CN
- China
- Prior art keywords
- resistor
- circuit
- pump
- buzzer
- pin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Infusion, Injection, And Reservoir Apparatuses (AREA)
Abstract
The utility model relates to the technical field of flow cytometry, in particular to a circuit for automatically filling liquid and clearing alarm sound when starting up based on the flow cytometer, which comprises an MCU circuit, wherein a singlechip U1 is used for realizing the main control of a system; a buzzer circuit including a buzzer for providing an alarm sound; a delay circuit for delaying power supply to the buzzer; a voltage switching circuit for switching a working voltage supplied to the buzzer connection; the liquid level detection circuit is used for connecting the sensor to detect the liquid level of the sheath liquid; the pump control circuit is used for controlling whether the pump works or not; and the pump driving circuit is used for controlling the working voltage of the pump so as to control the flow rate of the sheath liquid filled by the pump.
Description
Technical Field
The utility model relates to the technical field of flow cytometry, in particular to a circuit for clearing automatic liquid injection and startup alarm based on a flow cytometer.
Background
Flow cytometers are devices that automatically analyze and sort cells. It can quickly measure, store and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in liquid, and can select specified cell subsets according to the preselected parameter range. In the flow cytometer, sheath fluid is necessary, the conventional flow cytometer is usually performed by a manual filling method when the sheath fluid is insufficient at present, and cannot be automatically operated, so that the labor intensity of an operator is increased.
Disclosure of Invention
The utility model provides a circuit for automatically injecting liquid and clearing alarm sound when starting up based on a flow cytometer, which can realize automatic injection of sheath liquid, has a simple control circuit structure and reduces the cost, and aims to solve the problems that the sheath liquid injection in the existing loss cytometer needs manual control, the labor intensity is increased, and a circuit for controlling the alarm when starting up is complex and the cost is high.
The technical scheme is as follows: a circuit for automatically injecting liquid and clearing alarm sound when starting up based on a flow cytometer comprises an MCU circuit, a singlechip U1 is used for realizing the main control of the system;
a buzzer circuit including a buzzer for providing an alarm sound;
it is characterized in that it also comprises:
the delay circuit is used for delaying the power supply to the buzzer;
a voltage switching circuit for switching a working voltage supplied to the buzzer connection;
the liquid level detection circuit is used for connecting the sensor to detect the liquid level of the sheath liquid;
the pump control circuit is used for controlling whether the pump works or not;
and the pump driving circuit is used for controlling the working voltage of the pump so as to control the flow rate of the sheath liquid filled by the pump.
The delay circuit is further characterized by comprising a resistor R1, a capacitor C1, a diode D1 and a MOS transistor Q1, wherein one end of the resistor R1 is connected with the negative electrode of the diode D1 through a 12V power supply, the other end of the resistor R1 is connected with the positive electrode of the diode D1 and then connected with one end of the capacitor C1 and the gate end of the MOS transistor Q1, the other end of the capacitor C1 is grounded, and the source end and the drain end of the MOS transistor Q1 are grounded and connected with the voltage switching circuit;
the voltage switching circuit comprises a resistor R2, a resistor R3 and a MOS transistor Q2, one end of the resistor R2 is connected with the delay circuit, the other end of the resistor R2 is connected with one end of a resistor R3 and the grid end of the MOS transistor Q2, the other end of the resistor R3 and the source end of the MOS transistor Q2 are both connected with the 12V power supply, the drain end of the MOS transistor Q2 is connected with the anode of the buzzer, and the cathode of the buzzer is connected with the 3 pins of the single chip microcomputer U1;
the liquid level detection circuit comprises a resistor R4 and a resistor R5 which are connected with a sheath liquid monitoring sensor, and the resistor R4 and the resistor R5 are respectively connected with a pin 19 and a pin 18 of the singlechip U1;
the pump control circuit comprises a resistor R6 with one end connected with a 13-pin of the single chip microcomputer U1, the other end of the resistor R6 is connected with a grid end of a MOS tube Q4, a source end of the MOS tube Q4 is grounded, a drain end of the MOS tube Q4 is connected with a cathode of a light-emitting diode D2 and a 2-pin of the pump, and an anode of the diode D2 is connected with a 12V power supply through a resistor R8:
the pump driving circuit comprises a resistor R7 with one end connected with a pin 9 of the single-chip microcomputer U1, the other end of the resistor R7 is connected with a base electrode of a triode Q3, an emitter electrode of the triode Q3 is grounded, a collector electrode of the triode Q3 is connected with one end of a resistor R11, the other end of the resistor R11 is connected with one end of a resistor R9, one end of the resistor R10 and an ADJ pin of a voltage stabilizer U2, the other end of the resistor R10 is grounded, a VIN pin of the voltage stabilizer U2 is connected with a 12V power supply and a cathode of a diode D3, an OUT1 pin and an OUT2 pin of the voltage stabilizer U2 are connected with the other end of the resistor R9, an anode of the diode D3, a cathode of the diode D4, one end of a capacitor C3 and a pin 1 of the pump.
After the sheath liquid level detection circuit is adopted, the liquid level detection circuit is added to detect the liquid level of the sheath liquid, the pump control circuit and the pump driving circuit are used for realizing the automatic filling of the sheath liquid, the control of the buzzer alarm is directly realized by the delay circuit and the voltage switching circuit, the complex time sequence control circuit is omitted, the circuit structure is greatly simplified, and the cost is reduced.
Drawings
FIG. 1 is a schematic diagram of the present invention;
FIG. 2 is a schematic diagram of an MCU circuit;
FIG. 3 is a schematic diagram of a delay circuit;
FIG. 4 is a schematic diagram of a voltage switching circuit;
FIG. 5 is a schematic diagram of a buzzer circuit;
FIG. 6 is a schematic diagram of a liquid level detection circuit;
FIG. 7 is a schematic diagram of a pump control circuit;
fig. 8 is a schematic diagram of a pump drive circuit.
Detailed Description
Referring to fig. 1 to 8, the circuit for clearing an automatic liquid injection and power-on alarm based on the flow cytometer comprises an MCU circuit, a single chip microcomputer U1 is used for realizing the main control of the system, the power supply voltage is 5V, the use model is PIC16F690-I/P, the single chip microcomputer U1 in the application has the function of simply receiving sheath liquid level signals and outputting alarm signals and pump control signals, the control is the basic function of the single chip microcomputer, and a software method is not improved.
The buzzer circuit comprises a buzzer BUZZ for providing alarm sound, and a negative electrode of the buzzer circuit is connected with a pin 3 of the singlechip U1.
The delay circuit is used for delaying power supply to the buzzer and comprises a resistor R1, a capacitor C1, a diode D1 and a MOS tube Q1, one end of the resistor R1 is connected with the negative electrode of the diode D1 through a 12V power supply, the other end of the resistor R1 is connected with the positive electrode of the diode D1 and then connected with one end of the capacitor C1 and the grid end of the MOS tube Q1, the other end of the capacitor C1 is grounded, and the source end and the drain end of the MOS tube Q1 are grounded and connected with a voltage switching circuit; the voltage switching circuit is used for switching working voltage supplied to the connection of the buzzer and comprises a resistor R2, a resistor R3 and an MOS transistor Q2, one end of the resistor R2 is connected with the delay circuit, the other end of the resistor R2 is connected with one end of a resistor R3 and the grid end of the MOS transistor Q2, the other end of the resistor R3 and the source end of the MOS transistor Q2 are both connected with a 12V power supply, and the drain end of the MOS transistor Q2 is connected with the anode of the buzzer BUZZ and used for supplying power to the buzzer BUZZ; the delay starting voltage of the MOS tube Q1 is provided by using RC delay of the resistor R1 and the capacitor C1, the input voltage is delayed by the resistor R2 and the resistor R3 and is supplied to the buzzer BUZZ, the voltage stored by the capacitor C1 of the grid electrode of the MOS tube Q1 is quickly released by the diode D1 after the power-off, the circuit structure is simple, and good cost benefit is brought.
The power supply delay of the buzzer is adjustable, the fact that the power supply of the buzzer is started is guaranteed, the MCU normally supplies power after the signal provided by the MCU is completed, and therefore the problem that transient buzzer sounds when the equipment is started due to the fact that the MCU does not normally output signals when the MCU is started and the buzzer normally supplies power is solved, and the equipment is made to be more reliable.
The liquid level detection circuit is used for being connected with the sensor to detect the liquid level of the sheath liquid, and comprises a resistor R4 and a resistor R5 which are connected with the sheath liquid monitoring sensor, wherein the resistor R4 and the resistor R5 are respectively connected with a pin 19 and a pin 18 of the single chip microcomputer U1 to respectively detect the shortage of the liquid level of the sheath liquid and the fullness of the liquid level of the sheath liquid.
The pump control circuit is used for controlling whether the pump works or not, and comprises a resistor R6, one end of the resistor R3556 is connected with a pin 13 of the single chip microcomputer U1, the other end of the resistor R6 is connected with a grid end of a MOS tube Q4, a source end of the MOS tube Q4 is grounded, a drain end of the MOS tube Q4 is connected with a cathode of a light-emitting diode D2 and a pin 2 of the pump, an anode of the light-emitting diode D2 is connected with a 12V power supply through a resistor R8, and the light-emitting diode D2 only controls whether the pump works normally or not.
Pump drive circuit for thereby the velocity of flow of control pump filling sheath liquid, connect including one end the resistance R7 of the 9 feet of singlechip U1, triode Q3's base is connected to the resistance R7 other end, triode Q3's emitter ground, collecting electrode connecting resistance R11 one end, resistance R11 other end connecting resistance R9 one end, resistance R10 one end, stabiliser U2's ADJ foot, resistance R10 other end ground connection, 12V power and diode D3's negative pole are connected to stabiliser U2's VIN foot, stabiliser U2's OUT1 foot and OUT2 foot are connected the resistance R9 other end, diode D3's positive pole, diode D4's negative pole, electric capacity C3 one end, the 1 foot of pump. The diode D3 and the diode D4 are protection circuits, and provide limit values for the upper limit and the lower limit of the supply voltage of the PUMP, so that the damage caused by switching is avoided.
A sheath fluid monitoring sensor is used for providing a level signal to an MCU (microprogrammed control unit), the MCU sends a pump driving signal and a switch control signal according to the state after receiving the level signal, the pump driving signal is added to a base electrode of a triode Q3, an emitting electrode and a collecting electrode of a triode Q3 form a low resistance value, and the low resistance value is connected with a feedback resistor R9 in parallel, so that the output voltage of a linear power supply is increased, and the working voltage of the pump is provided to achieve the purpose of improving the liquid injection speed; when the liquid level is normal, the pump driving signal is at a low level, the triode Q3 is cut off, the output voltage of the linear power supply is low, and the low-speed liquid injection is realized when the working voltage of the pump is low; when the liquid level is not full, the switch control signal outputs high level to turn on the MOS tube Q4 to make the pump work; when the liquid level is full, the output low level turns off the MOS transistor Q4, and the pump voltage is not supplied to stop the operation. Making the instrument more automated and bringing good performance benefits.
Claims (6)
1. A circuit for automatically injecting liquid and clearing alarm sound when starting up based on a flow cytometer comprises an MCU circuit, a singlechip U1 is used for realizing the main control of the system;
a buzzer circuit including a buzzer for providing an alarm sound;
it is characterized in that it also comprises:
the delay circuit is used for delaying the power supply to the buzzer;
a voltage switching circuit for switching a working voltage supplied to the buzzer connection;
the liquid level detection circuit is used for connecting the sensor to detect the liquid level of the sheath liquid;
the pump control circuit is used for controlling whether the pump works or not;
and the pump driving circuit is used for controlling the working voltage of the pump so as to control the flow rate of the sheath liquid filled by the pump.
2. The automatic liquid injection and power-on alarm sound clearing circuit based on the flow cytometer as claimed in claim 1, wherein the delay circuit comprises a resistor R1, a capacitor C1, a diode D1 and a MOS transistor Q1, one end of the resistor R1 and a negative electrode of the diode D1 are connected to a 12V power supply, the other end of the resistor R1 is connected to an anode of the diode D1 and then connected to one end of the capacitor C1 and a gate end of the MOS transistor Q1, the other end of the capacitor C1 is grounded, and a source end and a drain end of the MOS transistor Q1 are connected to the ground and the voltage switching circuit.
3. The automatic liquid injection and power-on alarm sound clearing circuit based on the flow cytometer as claimed in claim 2, wherein the voltage switching circuit comprises a resistor R2, a resistor R3 and a MOS transistor Q2, one end of the resistor R2 is connected to the delay circuit, the other end of the resistor R2 is connected to one end of a resistor R3 and a gate end of the MOS transistor Q2, the other end of the resistor R3 and a source end of the MOS transistor Q2 are both connected to the 12V power supply, a drain end of the MOS transistor Q2 is connected to an anode of the buzzer, and a cathode of the buzzer is connected to 3 pins of the single chip microcomputer U1.
4. The automatic liquid injection and power-on alarm sound clearing circuit based on the flow cytometer as claimed in claim 3, wherein the liquid level detection circuit comprises a resistor R4 and a resistor R5 connected to the sheath liquid monitoring sensor, and the resistor R4 and the resistor R5 are respectively connected to the pin 19 and the pin 18 of the single chip microcomputer U1.
5. The automatic liquid injection and power-on alarm sound clearing circuit based on the flow cytometer as claimed in claim 4, wherein the pump control circuit comprises a resistor R6 with one end connected to the 13 pin of the single chip microcomputer U1, the other end of the resistor R6 is connected to the gate end of a MOS transistor Q4, the source end of the MOS transistor Q4 is grounded, the drain end of the MOS transistor Q4 is connected to the cathode of a light emitting diode D2 and the 2 pin of the pump, and the anode of the diode D2 is connected to a 12V power supply through a resistor R8.
6. The automatic liquid injection and power-on alarm sound clearing circuit based on the flow cytometer as claimed in claim 5, wherein the pump driving circuit comprises a resistor R7 with one end connected to the 9 pin of the single chip microcomputer U1, the other end of the resistor R7 is connected to the base of a transistor Q3, the emitter of the transistor Q3 is connected to ground, the collector of the transistor Q3 is connected to one end of a resistor R11, the other end of the resistor R11 is connected to one end of a resistor R9, one end of the resistor R10 and the ADJ pin of a voltage regulator U2, the other end of the resistor R10 is connected to ground, the VIN pin of the voltage regulator U2 is connected to a 12V power supply and the cathode of a diode D3, and the OUT1 pin and OUT2 pins of the voltage regulator U2 are connected to the other end of the resistor R9, the anode of the diode D3, the cathode of the diode D4, one end of a capacitor C3 and the pin 1 pin of the pump.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122738521.5U CN216526803U (en) | 2021-11-10 | 2021-11-10 | Automatic liquid injection and starting alarm clearing circuit based on flow cytometer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122738521.5U CN216526803U (en) | 2021-11-10 | 2021-11-10 | Automatic liquid injection and starting alarm clearing circuit based on flow cytometer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216526803U true CN216526803U (en) | 2022-05-13 |
Family
ID=81529758
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122738521.5U Active CN216526803U (en) | 2021-11-10 | 2021-11-10 | Automatic liquid injection and starting alarm clearing circuit based on flow cytometer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216526803U (en) |
-
2021
- 2021-11-10 CN CN202122738521.5U patent/CN216526803U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN203326671U (en) | Control circuit for electronic cigarette case | |
| CN206820644U (en) | Power output control circuit and power circuit | |
| CN202837425U (en) | Low-power consumption direct-current electronic load | |
| CN206061746U (en) | Automatic irrigation controller | |
| CN115199499A (en) | Solar water pump device for orchard irrigation | |
| CN216526803U (en) | Automatic liquid injection and starting alarm clearing circuit based on flow cytometer | |
| CN207460003U (en) | A kind of rearrangeable switch power supply based on BUCK circuits | |
| CN104808561B (en) | Multistate switch state collecting device and method | |
| CN206533171U (en) | The charging device of voltage comparator circuit and battery | |
| CN104955251A (en) | Voltage detecting circuit and lamp | |
| CN204334394U (en) | Direct current motor drive circuit | |
| CN203734586U (en) | Direct-current motor current-limiting protection circuit | |
| CN206364587U (en) | A kind of dual power supply not power down switches power supply circuit | |
| CN203324366U (en) | Power supply voltage detection circuit | |
| CN205355781U (en) | Area is to ground short -circuit protection's high limit drive circuit | |
| CN212115163U (en) | Control circuit for OEMS | |
| CN204142931U (en) | A kind of accumulator auto charge and discharge and capacity checking apparatus | |
| CN205069083U (en) | Overshoot prevention circuit | |
| CN108199446A (en) | Circuit and soft start equipment are protected in lithium battery trickle charge | |
| CN103927918B (en) | Automatic pumping demonstration device is controlled using triode | |
| CN103347348B (en) | Solar LED streetlight controller | |
| CN208276311U (en) | A kind of intelligent elevated controller of Z axis on wire cutting machine | |
| CN221824024U (en) | Water pump is from water circuit that cuts off power supply | |
| CN210299094U (en) | Automatic controller circuit module for fish tank aerator | |
| CN215599537U (en) | Urban afforestation irrigation intelligent control system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |