CN216493032U

CN216493032U - Cell cryopreservation tube

Info

Publication number: CN216493032U
Application number: CN202220291023.2U
Authority: CN
Inventors: 李海波; 陈城
Original assignee: Shanghai Xinpeptide Biotechnology Co ltd
Current assignee: Shanghai Xinpeptide Biotechnology Co ltd
Priority date: 2022-02-08
Filing date: 2022-02-08
Publication date: 2022-05-13
Anticipated expiration: 2032-02-08

Abstract

A cell cryopreservation tube comprising: the tube body is open at one end and closed at the other end; the cover is arranged at the open end of the tube body and comprises a cover body, a sealing layer and an easily breaking block; the sealing layer is arranged at the top of the cover body and seals the opening, and the easy-breaking block is arranged at the top of the cover body in a breaking-off manner and shields one side of the sealing layer facing the outer side of the cover body; the connecting surface of the easy-breaking block and the cover body is polygonal. According to the cell freezing tube, the tube body, the cover and the sealing layer thereof are matched with the easily-breaking block, the easily-breaking block is broken when the cell freezing tube is taken, the sealing layer is exposed at the moment, and the needle of the injector can pierce the sealing layer and penetrate into the tube body to complete cell taking; the easy-breaking block is designed to be conveniently broken by hands, but is not easy to be circumferentially twisted, so that the easy-breaking block cannot be accidentally broken no matter in the production, transportation and use links of the cell cryopreservation tube, particularly when the cover is rotated, and production and use accidents are reduced.

Description

Cell cryopreservation tube

[ technical field ] A method for producing a semiconductor device

The utility model relates to the technical field of cryopreservation tubes, in particular to a cell cryopreservation tube.

[ background of the utility model ]

Cell cryopreservation is one of the main methods for cell preservation, and the cell is placed in liquid nitrogen at the temperature of 196 ℃ below zero for low-temperature preservation by using a cryopreservation technology, so that the cell can be temporarily separated from a growth state and the cell characteristics can be preserved. The cryopreservation tube is a container for storing strains in a laboratory, is used for cell transfer, and can be used only after being recovered in a GMP laboratory when cells cryopreserved in the tube are directly used as medicines for clinical application.

However, the cap of the existing cryopreservation tube is made of all plastic, so that the syringe cannot be inserted into the tube for extraction, and the cryopreservation tube is inconvenient for clinical use. To this end, we have devised a cell cryopreservation tube to solve the above problems.

[ Utility model ] content

The utility model aims to solve the defects in the prior art and provides a cell cryopreservation tube.

In order to achieve the purpose, the utility model adopts the following technical scheme:

a cell cryopreservation tube comprising:

the tube body is open at one end and closed at the other end;

the cover is arranged at the open end of the tube body and comprises a cover body, a sealing layer and an easily breaking block; the sealing layer is arranged at the top of the cover body and seals the opening, and the easy-breaking block is arranged at the top of the cover body in a breaking-off manner and shields one side of the sealing layer facing the outer side of the cover body;

the connecting surface of the easy-breaking block and the cover body is polygonal.

In one embodiment, the connection surface between the easy-breaking block and the cover body is triangular.

In one embodiment, the breaking-off facilitating block is in a triangular prism shape, and the bottom surface of the prism is a connection surface with the cover body.

In one embodiment, the sealing layer is circular, and the connecting surface of the easy-breaking block is a circle inscribed polygon inscribed in the sealing layer.

In one embodiment, the easily-breaking block is integrally injection-molded with the cover body.

In one embodiment, the sealing layer is a rubber gasket.

In one embodiment, the cover is in threaded connection with the tube, wherein the open end of the tube is provided with an external thread, and the inner side wall surface of the cover body is provided with an internal thread corresponding to the external thread.

In one embodiment, the outer side wall surface of the cover body is provided with an anti-slip groove.

In one embodiment, the outer wall surface of the tube body is provided with scale marks and is fixed with a writing panel.

In one embodiment, the method further comprises the following steps:

the backup pad is located the blind end of body, backup pad one end with the body is fixed, the other end to the body bottom direction extends and surpasss the body bottom, the backup pad with fixedly connected with stiffener between the body.

Compared with the prior art, the utility model has the following beneficial effects: according to the cell freezing tube, the tube body, the cover, the sealing layer and the easily-breaking block are matched with each other, the easily-breaking block is broken when the cell freezing tube is taken, the sealing layer is exposed at the moment, the needle of the injector can pierce the sealing layer and penetrate into the tube body to complete cell taking, the operation is simple, the size is small, cell transfer is facilitated, repeated freezing and thawing are not needed when the cell freezing tube is used, damage to cells can be effectively reduced, and the cell freezing tube is convenient for clinical use; the easy-breaking block is designed to be conveniently broken by hands, but is not easy to be circumferentially twisted, so that the easy-breaking block cannot be accidentally broken no matter in the production, transportation and use links of the cell cryopreservation tube, particularly when the cover is rotated, and production and use accidents are reduced.

[ description of the drawings ]

FIG. 1 is a perspective view of a lid of a cell cryopreservation tube according to the present invention;

FIG. 2 is a bottom view of the lid of the cell cryopreservation tube according to the present invention;

FIG. 3 is a perspective view of the tube of the cell cryopreservation tube according to the present invention.

[ detailed description ] embodiments

It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict.

In the description of the present application, it is to be understood that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientation or positional relationship indicated in the drawings for convenience in describing the present application and for simplicity in description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed in a particular orientation, and be operated in a particular manner, and are not to be considered limiting of the scope of the present application. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the utility model of the present application, the meaning of "a plurality" is two or more unless otherwise specified.

In the description of the present application, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art through specific situations.

Referring to fig. 1-3, a cell freezing tube includes a tube body 10 and a cover 20. The tube body 10 is used for accommodating cells to be stored and transferred, the tube body 10 is in a straight tube shape, one end of the tube body is open, the other end of the tube body is closed, and the cells are taken out through the open end of the tube body 10. A cap 20 is provided at the open end of the tube 10 to close the tube 10, and the cap 20 of this embodiment includes a cap body 21, a sealing layer 22, and a frangible block 23. Wherein, the cover body 21 comprises a top part and a ring-shaped side wall, the top part of the cover body 21 is provided with an opening, and the opening can comprise all or part of the top area of the cover body 21; the sealing layer 22 is provided on the top of the lid body 21 and seals the opening thereof. The easily breaking block 23 is disposed on the top of the cover 21 in an easily detachable manner, that is, the easily breaking block 23 is disposed on the top of the cover 21 in an openable manner, and the connection between the easily breaking block 23 and the cover 21 is completely or partially separated by applying a force to one side of the easily breaking block 23. The easy-breaking block 23 is configured to shield one side of the sealing layer 22 facing the outer side of the cover 21, and when the easy-breaking block 23 is not broken, the sealing layer 22 is completely shielded and not exposed to the outside, so as to prevent pollution and damage; after the easily breaking pieces 23 are broken, the sealing layer 22 is partially or completely exposed, so that a needle or the like can be inserted through the sealing layer 22 to access the cells in the tube 10. The connection surface of the easy-breaking block 23 and the cover 21 is polygonal, such as triangular, rectangular, etc. The polygonal easy-breaking block 23 is easy to break when acting force in one side direction is applied, and is not easy to break away from the cover body 21 when acting force in the circumferential direction is applied, so that accidental breakage of the easy-breaking block 23 caused by a production link or a situation of screwing the cover 20 due to misoperation is prevented.

In one embodiment, the connection surface of the easy-breaking block 23 and the cover 21 is triangular. The triangular connecting surface has stronger structural stability, is easy to design and manufacture, has lower cost, is difficult to separate from the cover body 21 accidentally due to the easy breaking block 23, and has better use effect.

In one embodiment, the breaking-off facilitating block 23 has a triangular prism shape, and the bottom surface of the prism is the connection surface with the cover 21. The triangular prism type easy-breaking block 23 has stronger structural stability, is easy to design and manufacture, has lower cost, is not easy to separate from the cover body 21 accidentally, and has better use effect. The side wall of the prism can be a frosted surface to enhance the friction force during the breaking off and prevent slipping; the edges and corners of the prism need to be ground flat or made into round corners to prevent fingers from being cut.

In one embodiment, the sealing layer 22 is circular, and the connection surface of the easy-breaking block 23 is a polygon inscribed in the circle of the sealing layer 22. After the breakable blocks 23 are broken, the sealing layer 22 is exposed, and the non-sealing layer 22 on the top of the cover 21 is not exposed, so as to prevent contamination and prevent the needle tip from being mixed with the sealing layer 22 and not inserted into the sealing layer 22. Of course, in other embodiments, the connection surface of the breaking-off blocks 23 may be located within the sealing layer 22 and not necessarily inscribed.

In one embodiment, the breakable blocks 23 are integrally injection-molded with the cover 21. It should be noted that, during injection molding, the connection between the cover body 21 and the easy-breaking block 23 is configured to be weak, so that the easy-breaking block 23 is separated from the cover body 21.

In one embodiment, the sealing layer 22 is a rubber gasket.

In one embodiment, the cap 20 is screwed with the tube 10, wherein the open end of the tube 10 is provided with an external thread, and the inner side wall surface of the cap body 21 is provided with an internal thread corresponding to the external thread. When needed, the cover 20 can be screwed to the outer side wall of the open end of the tube body 10, the sealing performance in the tube body 10 is ensured, and only the cover 20 needs to be rotated reversely during disassembly, so that the integral assembly and disassembly of the cover 20 are facilitated. Meanwhile, the direction of the acting force required by the cap 20 screwed in and out of the thread is not consistent with that of the polygonal easy-breaking block 23, so that the generation of misoperation is reduced.

In one embodiment, the cover 21 is provided with an anti-slip groove 24 on its outer wall surface. The anti-slip groove 24 increases friction to facilitate rotation of the lid 20.

In one embodiment, the outer wall of the tube 10 is provided with graduations 11 and a writing surface plate 12 is fixed thereto. The scale marks 11 are convenient for users to visually know the cell stock in the tube body 10, and the writing panel 12 is convenient for the users to mark each cell cryopreservation tube.

The closed end of the cell freezing tube is generally spherical and is not easy to be directly placed. In one embodiment, the cell cryopreservation tube further comprises a support plate 30. The blind end of body 10 is located to backup pad 30, and backup pad 30 one end is fixed with body 10, and the other end extends and surpasss the body 10 bottom to body 10 bottom direction for cell cryopreserving pipe accessible backup pad 30 is upright to be placed. A reinforcing rod 31 is fixedly connected between the supporting plate 30 and the tube 10 to reinforce the structure of the supporting plate 30.

The embodiment of the application also discloses a using process of the cell cryopreservation tube: when cells in the tube 10 need to be taken out, the breakable block 23 can be broken, the breakable block 23 is separated from the cover 21, and at least a part of the sealing layer 22 is correspondingly exposed; inserting the needle of the syringe for taking cells into the sealing layer 22, wherein the needle can penetrate through the sealing layer 22 and penetrate into the tube 10; the cell in the tube 10 can be taken out by operating the syringe.

In light of the foregoing description of the preferred embodiments according to the present application, it is to be understood that various changes and modifications may be made without departing from the spirit and scope of the utility model. The technical scope of the present application is not limited to the content of the specification, and must be determined according to the scope of the claims.

Claims

1. A cell cryopreservation tube, comprising:

the tube body is open at one end and closed at the other end;

2. The cell cryopreservation tube of claim 1, wherein the connection surface between the breakable block and the cover is triangular.

3. The cell cryopreservation tube of claim 1, wherein the easily breaking block is in a triangular prism shape, and the bottom surface of the prism is a connection surface with the cover body.

4. The cell cryopreservation tube of claim 1, wherein the sealing layer is circular, and the connecting surface of the easily-breaking block is a circle inscribed polygon inscribed in the sealing layer.

5. The cell cryopreservation tube of claim 1, wherein the easily-breaking block is integrally injection-molded with the cover body.

6. The cell cryopreservation tube of claim 1, wherein the sealing layer is a rubber gasket.

7. The cell cryopreservation tube of claim 1, wherein the cover is in threaded connection with the tube body, wherein the open end of the tube body is provided with an external thread, and the inner side wall surface of the cover body is provided with an internal thread corresponding to the external thread.

8. The cell cryopreservation tube of claim 1, wherein an anti-slip groove is formed on the outer side wall surface of the cover body.

9. The cell freezing tube according to claim 1, wherein the outer wall of the tube body is provided with graduations and a writing panel is fixed thereto.

10. The cell cryopreservation tube of claim 1, further comprising:

the backup pad is located the blind end of body, backup pad one end with the body is fixed, the other end to body bottom direction extends and surpasss the body bottom, the backup pad with fixedly connected with stiffener between the body.