CN216439356U - Mouse back skin pleat window model system - Google Patents
Mouse back skin pleat window model system Download PDFInfo
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- CN216439356U CN216439356U CN202122404179.5U CN202122404179U CN216439356U CN 216439356 U CN216439356 U CN 216439356U CN 202122404179 U CN202122404179 U CN 202122404179U CN 216439356 U CN216439356 U CN 216439356U
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Abstract
The utility model discloses a rat dorsal spine skin fold window chamber model system which comprises a window chamber support and a temperature control module, wherein the window chamber support comprises an upper frame and a lower frame, a clamping groove is formed in the surface of one side of the lower frame, and the temperature control module is arranged in the clamping groove; the upper frame is provided with a detachable window; the upper frame and the lower frame are fixed through bolts and/or stitches; the temperature control module comprises a temperature control host, a temperature sensor and a semiconductor refrigerator, wherein the temperature control host is connected to the temperature sensor, the temperature control host is connected to the semiconductor refrigerator, and the temperature sensor is also connected to the semiconductor refrigerator; the scheme can realize window microcirculation constant temperature control, can realize various temperature control conditions, meets the requirement of researching the microcirculation response characteristics under various temperature control conditions, and can be widely applied to the technical field of biological experiment equipment.
Description
Technical Field
The utility model relates to the technical field of biological experimental equipment, in particular to a rat dorsal spine skin fold window model system.
Background
Microcirculation is the most basic structure and function unit of the circulatory system, and plays an important role in maintaining normal physiological functions of animals and human bodies. The temperature response characteristic is an important physiological characteristic of microcirculation and is an important basis for the formulation of cold and heat treatment schemes for pathological microcirculation (such as tumor microcirculation). The transparent chamber combined with microscope for living tissue microcirculation is a very important experimental method in microcirculation research, wherein a dorsal skinfold window chamber model suitable for rodents is adopted and improved by a plurality of researchers.
However, the prior art schemes still have great limitations in studying the temperature response characteristics of the microcirculation. Firstly, the window chamber model support in the prior art is designed to be large in size, so that the load burden of small rodents such as mice and nude mice is increased, and the normal activity of the rodents is influenced when the window chamber model support is arranged on the back of the mouse and the nude mice; secondly, the existing scheme adopts locking buckles and other modes to install and fix the cover glass, and the scheme is inconvenient to disassemble and repeatedly install, so that the operation of a microcirculation experiment is influenced; thirdly, the existing spine skin fold window model does not have the automatic temperature control function, the observation of the temperature response characteristics of the microcirculation under different cold and hot conditions cannot be independently realized, and the cold and hot temperature stimulation of the microcirculation around the window can be realized only by utilizing other temperature adjusting devices and media such as water, alcohol and the like in some experimental researches. Therefore, the whole experimental device becomes very complex, the temperature cannot be accurately controlled, and the rapid switching of cold and heat stimulation is difficult to realize, so that the use in the microcirculation research is limited.
SUMMERY OF THE UTILITY MODEL
To solve one of the above technical problems, the present invention aims to: a more portable window chamber model system for the study of the temperature response characteristics of the microcirculation is provided.
The technical scheme adopted by the utility model is as follows:
the utility model provides a rat dorsal spine skin fold window chamber model system which comprises a window chamber support and a temperature control module, wherein the window chamber support comprises an upper frame and a lower frame, a clamping groove is formed in the surface of one side of the lower frame, and the temperature control module is arranged in the clamping groove; the upper frame is provided with a detachable window; the upper frame and the lower frame are fixed through bolts and/or screws;
the temperature control module comprises a temperature control host, a temperature sensor and a semiconductor refrigerator, wherein the temperature control host is connected to the temperature sensor, the temperature control host is connected to the semiconductor refrigerator, and the temperature sensor is further connected to the semiconductor refrigerator.
In some optional embodiments, the upper frame is provided with a window hole protruding towards one side surface, the detachable window comprises a fixing ring and a cover glass, the edge of the window hole is provided with a first groove and a second groove, the cover glass is arranged on one side, close to the lower frame, of the cover glass, the edge of the cover glass is matched and fixed with the first groove, the fixing ring is arranged on one side, away from the lower frame, of the window hole, the edge of the fixing ring is matched and fixed with the second groove, and the cover glass is tightly attached to the fixing ring.
In some optional embodiments, the fixing ring is a plastic ring, the ring is provided with a notch, and two ends of the plastic ring close to the notch are provided with openings.
In some optional embodiments, the surface of the upper frame is provided with a plurality of first bolt openings and a plurality of first suture openings; the surface of the lower frame is provided with a plurality of second bolt ports and a plurality of second suture ports; the first bolt hole and the second bolt hole are matched with each other after the upper frame and the lower frame are attached; the first suture opening and the second suture opening are mutually matched after the upper frame and the lower frame are attached.
In some optional embodiments, the temperature control host comprises a microcontroller, a main circuit board, and an H-bridge circuit;
the microcontroller is connected to the main circuit board; the main circuit board is connected to the temperature sensor and the H-bridge circuit; the H-bridge circuit is also connected to the semiconductor refrigerator.
In some optional embodiments, the temperature control host further comprises a power module, a physical key module and a display module;
the power module is connected to the main circuit board, the physical key module is connected to the main circuit board, and the display module is connected to the main circuit board.
In some optional embodiments, the upper frame is a titanium alloy frame, and the lower frame is a hard polymer material frame.
In some optional embodiments, the upper frame is provided with a first fixing bracket, the length of the first fixing bracket is greater than the side length of the upper frame, and the first fixing bracket is integrally formed with the upper frame; the lower frame is provided with a second fixed support, the length of the second fixed support is greater than the side length of the lower frame, and the second fixed support and the lower frame are integrally formed; the first fixing support and the second fixing support are equal in length.
The utility model has the beneficial effects that: the technical scheme of the application mainly comprises a window chamber support and a temperature control module, wherein the window chamber support is simple in structure, small in size and weight and mainly composed of an upper frame and a lower frame; the back of the lower frame is provided with a temperature control module clamping groove for placing a temperature control module component. The temperature control module comprises a temperature control host, a temperature sensor and a TEC thermoelectric refrigerating piece, has various working modes of one-way refrigeration, one-way heating and cold and hot alternation, realizes window microcirculation constant temperature control, can realize various temperature control conditions, and meets the requirement of microcirculation response characteristic research under various temperature control conditions.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
FIG. 1 is a schematic structural view of an upper frame in a rat dorsal ridge skinfold window model system according to the present invention;
FIG. 2 is a schematic structural diagram of a lower frame in a rat dorsal spine skin fold window model system according to the present invention;
FIG. 3 is a schematic view of the structure of a cover glass-free window frame window and a cover glass-mounted window of the present invention;
FIG. 4 is a schematic view of a retaining ring according to the present invention;
FIG. 5 is a view showing the combination of the upper frame and the lower frame of the window chamber bracket according to the present invention;
FIG. 6 is a functional block diagram of a window compartment bracket of a temperature control module according to the present invention.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
In the description of the present invention, it is to be understood that the terms "length," "upper," "lower," "front," "rear," "left," "right," "top," "inner," "outer," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the present invention and for simplicity in description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be construed as limiting the present invention. Furthermore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Aiming at the defects of a rat dorsal ridge skin fold window model used for the observation and research of the microcirculation in vivo experiment at present, the utility model provides a window model system for the temperature response characteristic research of the microcirculation.
As shown in fig. 1 and fig. 2, the rat dorsal skinfold window model system provided by the utility model comprises a window chamber bracket and a temperature control module, wherein the window chamber bracket comprises an upper bracket and a lower bracket, a gap is formed between the upper bracket and the lower bracket and is used for clamping the back skin of an animal and can be fixed through bolts and/or sutures, and the outline shape and the size of the upper bracket in the system window chamber bracket are consistent with those of the lower bracket.
In the system of the embodiment, a temperature control module clamping groove is formed in the back face of the lower frame of the window chamber support and used for placing components of the temperature control module. The temperature control module in the embodiment can be used for various working modes of one-way refrigeration, one-way heating and cold-hot alternation, and can realize window microcirculation constant temperature control; the temperature control module in the embodiment includes a temperature control host, a temperature sensor, and a semiconductor Cooler (TEC); the temperature control host is connected to the temperature sensor, the semiconductor refrigerator and the semiconductor refrigerator, and the temperature sensor is further connected to the semiconductor refrigerator. The temperature control host is mainly used for acquiring the temperature of the temperature sensor, carrying out visual display and triggering a corresponding control signal according to the acquired temperature control instruction of the operator so as to control the semiconductor refrigerator to heat or refrigerate.
In some alternative embodiments, the upper frame is provided with a first fixed bracket, the length of the first fixed bracket is greater than the side length of the upper frame, and the first fixed bracket is integrally formed with the upper frame; the lower frame is provided with a second fixed support, the length of the second fixed support is greater than the side length of the lower frame, and the second fixed support and the lower frame are integrally formed; the first fixing support and the second fixing support are equal in length;
the first fixing supports of the embodiment are portions obtained by extending a certain edge of the rectangular upper frame, and correspondingly, the second fixing supports are portions obtained by extending a certain edge of the lower frame.
Illustratively, as shown in fig. 1, a pair of parallel edges of a rectangular window support upper frame is extended, so that the upper frame is in an I shape as a whole; as shown in FIG. 2, the lower frame has the same contour shape and size as the upper frame, is also in an I shape, and has the advantages of simple structure and small volume and weight.
In some alternative embodiments, the upper frame of the window chamber bracket is a titanium alloy frame, and the lower frame is a hard high polymer material frame;
in the embodiment, the upper frame is made of a titanium alloy material, and the alloy material has high specific strength and can keep the stability of the observation window chamber; the lower frame has the same contour and size as the upper frame, is made of hard high polymer material with low heat conductivity, has light weight and is beneficial to the temperature control of a central area. In addition, as shown in fig. 2, a square temperature control module slot is formed in the center of the lower frame for placing a temperature sensor and a TEC plate of the temperature control module.
In some alternative embodiments, the upper frame is provided with a viewing window hole protruding towards one side surface, the detachable viewing window comprises a fixing ring and a cover glass, the edge of the viewing window hole is provided with a first groove and a second groove, the cover glass is arranged on one side of the cover glass close to the lower frame, the edge of the cover glass is matched and fixed with the first groove, the fixing ring is arranged in the viewing window hole and is arranged on one side far away from the lower frame, the edge of the fixing ring is matched and fixed with the second groove, and the cover glass is tightly attached to the fixing ring;
the first groove is used for placing the cover glass, and the second groove is used for placing the fixing ring.
Specifically, a circular window hole in the center of an upper frame of the window bracket is protruded, and an inclined plane and a groove are designed on the edge of the window hole; when the device is installed, the cover glass and the fixing ring are sequentially placed in the window hole. As shown in fig. 3, a non-coverslipped window frame window 310 and a coverslipped window 311; in fig. 3, the left side of the window frame window 310 without the cover glass and the left side of the window 311 with the cover glass is close to the lower frame or the end close to the skin fold of the back ridge of the mouse, and the right side is far away from the lower frame or the end far away from the skin fold of the back ridge of the mouse; wherein, the edge of the cover glass 301 is designed as an inclined plane, and is placed at the bottom of the window hole when being installed, the edge is contacted with the inclined plane of the window hole, and a gap is left between the lower surface of the cover glass and the wound and can not be directly contacted with tissues; the retaining ring 302 is made of plastic and has an outer diameter that is comparable to or slightly larger than the groove so that an interference fit is formed when installed. The fixing ring 302 can be fastened in the groove so as to fix the cover glass, so that the formed view window has better sealing performance and is not easy to infect the exposed tissues; the disassembly is convenient for carrying out experimental operation; no contact with bare tissues and no man-made interference; the size of the viewing window can be adjusted, and the requirements of different observation visual fields are met.
In some alternative embodiments, the fixing ring in the window is a plastic ring, the ring is provided with a notch, and openings are formed at two ends of the plastic ring close to the notch.
Specifically, as shown in fig. 4, the fixing ring is made of plastic, and the outer diameter of the fixing ring is equal to or slightly larger than the groove, so that an interference fit state can be formed during installation. A notch is reserved in the fixing ring, the tweezers are used for clamping two round openings near the notch during installation to enable the round openings to be slightly deformed and then placed in the groove, the tweezers are loosened after the round openings are placed, and the fixing ring can be fastened in the groove after restoring the original shape, so that the cover glass is fixed; when the sight window is disassembled, the two round openings near the notch are clamped by tweezers to be slightly deformed and then can be taken down; the fixing rings with different inner diameters are installed, and the size of the view window can be adjusted through the fixing rings.
In some alternative embodiments, the surface of the upper frame is provided with a plurality of first bolt openings and a plurality of first suture openings; the surface of the lower frame is provided with a plurality of second bolt ports and a plurality of second suture ports; the first bolt hole and the second bolt hole are matched with each other after the upper frame and the lower frame are jointed; the first suture opening and the second suture opening are mutually matched after the upper frame and the lower frame are attached.
Specifically, as shown in fig. 1, the surface of the upper frame is provided with 4 bolt openings 101 and 2 rows of suture openings 102; the lower frame and the upper frame are also provided with 4 bolt openings 201 and 2 rows of suture openings 202 at corresponding positions. As shown in fig. 5, the upper frame and the lower frame are combined, and the upper frame, the lower frame and the sandwiched dorsal skin are fastened together with bolts/nuts and stitches at the bolt openings and the stitch openings, respectively.
In some alternative embodiments, the temperature control host in the temperature control module includes a microcontroller, a main circuit board, and an H-bridge circuit.
Wherein the microcontroller is connected to the main circuit board; the main circuit board is connected to the temperature sensor and the H-bridge circuit; the H-bridge circuit is also connected to the semiconductor refrigerator.
In some optional embodiments, the temperature control host further comprises a power module, a physical key module and a display module;
the power supply module is connected to the main circuit board, the physical key module is connected to the main circuit board, and the display module is connected to the main circuit board.
Specifically, as shown in fig. 6, the temperature control host mainly comprises a microcontroller, a main circuit board, a key, a power supply, a display screen, an H-bridge circuit, and the like; the temperature control host has 3 working modes: one-way refrigeration, one-way heating and cold-hot alternate work; the working mode, the temperature control temperature and the working time are set through the keys. A microcontroller, physical keys and a display screen are integrated on the main circuit board; the physical key module is mainly used for selecting a working mode and setting temperature control temperature; the display module is mainly used for displaying a temperature control mode, a temperature control temperature and tissue temperature acquired in real time; the microcontroller is one of the cores of the temperature control module, and a proportional-differential-integral (PID) control algorithm for temperature control is embedded in the microcontroller to realize different refrigerating and heating temperature control requirements. The H-bridge circuit is connected with the main circuit board and is used for bidirectional control of output voltage; the temperature sensor is connected with the main circuit board, collects the temperature in real time and transmits the temperature back to the microcontroller; the TEC sheet is connected with the H bridge circuit and carries out refrigeration/heating according to the voltage output by the H bridge. When the temperature sensor is installed and used, the temperature sensor is fixed on the surface (the side contacting with tissues) of the TEC sheet and is embedded into the clamping groove of the temperature control module of the lower bracket of the bracket together.
With reference to fig. 1 to 6, the present invention provides three complete embodiments:
the embodiment I, the mouse dorsal spine skin fold window model system is applied to a morphological function observation experiment under normal microcirculation and normal temperature:
and (3) disinfection: sterilizing the dorsal skin fold window model support, the fastening bolt and the nut thereof;
anesthesia: the experimental mice were injected with the anesthetic in the abdominal cavity at a dose of 40mg/kg body weight.
Depilation: if the experimental animal is not a nude mouse, the back hair of the mouse should be cut short after anesthesia and subjected to a back depilation treatment with a chemical depilatory.
Scribing: under the prone posture of the experimental mouse, a pen is used as a marking line along the dorsal line of the experimental mouse, and then the skin of the dorsal ridge of the experimental mouse is lifted along the marking line;
determining the mounting position of the bracket: pressing the lower support of the support on one side of the lifted rat dorsal spine skin, adjusting the position of the support to ensure that 4 bolt holes and 2 rows of suture holes are in the range of lifting the skin, penetrating the cortex at the 4 bolt holes by using a medical needle, and then penetrating bolts;
determining the position of the window opening: sleeving the upper bracket of the bracket on 4 bolts from the other side of the lifted back skin of the rat, screwing nuts to position the bracket, marking an area needing to be perforated on the skin in the window hole of the upper bracket by using a pen, and then taking down the bracket;
the skin is cut and made in the stereoscopic window: and (4) shearing skin and subcutaneous tissues on one side along the marked perforation line by using medical scissors, and cleaning the connective tissues. Dripping phosphate protective agent into wound surface to keep it moist, and treating wound with antibiotic ointment to prevent infection;
the support mounting is fixed: respectively installing a lower bracket and an upper bracket on two sides of the lifted rat dorsal spine skin according to 4 opened bolt hole positions on the skin, tightly pressing the brackets on the two sides and the rat dorsal spine skin layer by using 4 bolts and nuts, and sewing and fixing the brackets and the dorsal spine skin by using a medical sewing needle;
the window is fixedly installed: and (3) placing a cover glass at the window hole of the mounted upper frame of the bracket, selecting a fixing ring with a proper size and specification according to experiment needs, and then clamping two round openings on the fixing ring by using forceps to place the fixing ring into the window hole groove for fixing.
And (3) microcirculation observation: and observing and testing the microcirculation shape and blood flow state of the sight window by using equipment such as a stereomicroscope, a laser speckle contrast imager and the like.
The second embodiment, this application mouse dorsal spine skin fold window model system is applied to tumour microcirculation growth observation experiment at room temperature:
and (3) disinfection: sterilizing the dorsal skin fold window model support, the fastening bolt and the nut thereof;
anesthesia: the experimental mice were injected with the anesthetic in the abdominal cavity at a dose of 40mg/kg body weight.
Depilation: if the experimental animal is not a nude mouse, the back hair of the mouse should be cut short after anesthesia and subjected to a back depilation treatment with a chemical depilatory.
Scribing: under the prone posture of the experimental mouse, a pen is used as a marking line along the dorsal line of the experimental mouse, and then the skin of the dorsal ridge of the experimental mouse is lifted along the marking line;
determining the mounting position of the bracket: pressing the lower support of the support on one side of the lifted rat dorsal spine skin, adjusting the position of the support to ensure that 4 bolt holes and 2 rows of suture holes are in the range of lifting the skin, penetrating the cortex at the 4 bolt holes by using a medical needle, and then penetrating bolts;
determining the position of the window opening: sleeving the upper bracket of the bracket on 4 bolts from the other side of the lifted back skin of the rat, screwing nuts to position the bracket, marking an area needing to be perforated on the skin in the window hole of the upper bracket by using a pen, and then taking down the bracket;
the skin is cut and made in the stereoscopic window: and (4) shearing skin and subcutaneous tissues on one side along the marked perforation line by using medical scissors, and cleaning the connective tissues. Dripping phosphate protective agent into wound surface to keep it moist, and treating wound with antibiotic ointment to prevent infection;
the support mounting is fixed: respectively installing a lower bracket and an upper bracket on two sides of the lifted rat dorsal spine skin according to 4 opened bolt hole positions on the skin, tightly pressing the brackets on the two sides and the rat dorsal spine skin layer by using 4 bolts and nuts, and sewing and fixing the brackets and the dorsal spine skin by using a medical sewing needle;
the window is fixedly installed: and (3) placing a cover glass at the window hole of the mounted upper frame of the bracket, selecting a fixing ring with a proper size and specification according to experiment needs, and then clamping two round openings on the fixing ring by using forceps to place the fixing ring into the window hole groove for fixing.
Tumor modeling: and taking down the fixing ring and the cover glass, implanting 4T1 cells into fascia tissues in the window on the back of the experimental mouse for tumor modeling, and mounting the cover glass and the fixing ring again after the experimental operation is finished. The experimental mice are fed and cultured in an aseptic isolation cage under proper environmental conditions, and the success of the model building can be judged after about 7 days through lump observation and morphological analysis of new blood vessels under a stereoscopic microscope.
And (3) observing tumor microcirculation: the regular tracking observation experiment is carried out on the microcirculation state and the blood flow state of the tumor at the sight window by utilizing equipment such as a stereomicroscope, a laser speckle contrast imager and the like.
In the third embodiment, the mouse dorsal spine skin fold window model system is applied to the research experiment of tumor microcirculation injury under cold and hot alternate treatment:
and (3) disinfection: sterilizing the dorsal skin fold window model support, the fastening bolt and the nut thereof;
anesthesia: the experimental mice were injected with the anesthetic in the abdominal cavity at a dose of 40mg/kg body weight.
Depilation: if the experimental animal is not a nude mouse, the back hair of the mouse should be cut short after anesthesia and subjected to a back depilation treatment with a chemical depilatory.
Scribing: under the prone posture of the experimental mouse, a pen is used as a marking line along the dorsal line of the experimental mouse, and then the skin of the dorsal ridge of the experimental mouse is lifted along the marking line;
determining the mounting position of the bracket: pressing the lower support of the support on one side of the lifted rat dorsal spine skin, adjusting the position of the support to ensure that 4 bolt holes and 2 rows of suture holes are in the range of lifting the skin, penetrating the cortex at the 4 bolt holes by using a medical needle, and then penetrating bolts;
determining the position of the window opening: sleeving the upper bracket of the bracket on 4 bolts from the other side of the lifted back skin of the rat, screwing nuts to position the bracket, marking an area needing to be perforated on the skin in the window hole of the upper bracket by using a pen, and then taking down the bracket;
the skin is cut and made in the stereoscopic window: and (4) shearing skin and subcutaneous tissues on one side along the marked perforation line by using medical scissors, and cleaning the connective tissues. Dripping phosphate protective agent into the wound surface to keep it moist, and treating the wound with antibiotic ointment to prevent infection;
the support mounting is fixed: respectively installing a lower bracket and an upper bracket on two sides of the lifted rat dorsal spine skin according to 4 opened bolt hole positions on the skin, tightly pressing the brackets on the two sides and the rat dorsal spine skin layer by using 4 bolts and nuts, and sewing and fixing the brackets and the dorsal spine skin by using a medical sewing needle;
the window is fixedly installed: and (3) placing a cover glass at the window hole of the mounted upper frame of the bracket, selecting a fixing ring with a proper size and specification according to experiment needs, and then clamping two round openings on the fixing ring by using forceps to place the fixing ring into the window hole groove for fixing.
Tumor modeling: and taking down the fixing ring and the cover glass, implanting 4T1 cells into fascia tissues in the window on the back of the experimental mouse for tumor modeling, and mounting the cover glass and the fixing ring again after the experimental operation is finished. The mice are fed with the culture experimental mice under the conditions of sterile isolation cages and proper environment, and the success of the model building can be judged after about 7 days through the tumor observation and the morphological analysis of the new vessels under a stereoscopic microscope.
Study of tumor microcirculation injury under cold and hot alternate treatment: 200 ml of 100nm rhodamine-labeled liposome suspension is injected into the body of the experimental mouse through the tail vein. Then, a TEC sheet (the surface of which is stuck with a temperature sensor) of the temperature control module is placed in a temperature control clamping groove of a lower support frame, a temperature control host is started, a refrigeration/heating alternative working mode is selected through a key, a refrigeration condition of 1 ℃ and a heating condition of 42 ℃ are set, the total working time is selected to be 1 hour, after the TEC sheet is started, 1 ℃ cold therapy is firstly carried out on the tumor microcirculation for 30 minutes, and then the TEC sheet is automatically switched to 42 ℃ heat therapy for 30 minutes. After the cold and heat treatments are alternated for 1 hour, the tumor microcirculation blood vessels are observed by utilizing a laser confocal microscope, and the extravasation condition of the liposome after the cold and heat treatments are alternately recorded. In order to compare the damage conditions of tumor microcirculation under different cold/heat treatment schemes, different working modes can be selected for comparison, such as 1 hour of pure cold treatment at 1 ℃ and 1 hour of pure heat treatment at 42 ℃.
In summary, compared with the prior art, the utility model has the following characteristics or advantages:
1. the rat dorsal spine skin fold window chamber model system has a special automatic temperature control function, has multiple working modes of one-way refrigeration, one-way heating and cold-hot alternation, can realize multiple temperature control conditions, and meets the requirement of microcirculation response characteristic research under the multiple temperature control conditions. The function is realized based on a TEC sheet and a PID control principle;
2. according to the mouse dorsal spine skin fold window model system, the viewing window is detachable and adjustable in size, so that long-term observation and experimental operation of microcirculation are facilitated; the window hole edge is optimally designed, and after the cover glass is installed, a gap is reserved between the bracket and the exposed tissue, so that the bracket cannot contact and press the wound, and the subcutaneous microcirculation environment is not interfered;
3. according to the rat dorsal spine skin fold window chamber model system, the whole support is I-shaped, the structure is simple, and the size and the weight are small. The upper bracket of the bracket is made of titanium alloy material, and the alloy material has high specific strength and can keep the stability of the viewing window; the lower frame is made of hard high polymer materials with low heat conductivity, so that the weight of the support can be further reduced, and the temperature control of a central area is facilitated.
In the description herein, references to the description of "one embodiment," "another embodiment," or "certain embodiments," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the utility model. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
While embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the utility model, the scope of which is defined by the claims and their equivalents.
Claims (8)
1. A rat dorsal spine skin fold window chamber model system is characterized by comprising a window chamber support and a temperature control module, wherein the window chamber support comprises an upper frame and a lower frame, a clamping groove is formed in the surface of one side of the lower frame, and the temperature control module is arranged in the clamping groove; the upper frame is provided with a detachable window; the upper frame and the lower frame are fixed through bolts and/or stitches;
the temperature control module comprises a temperature control host, a temperature sensor and a semiconductor refrigerator, wherein the temperature control host is connected to the temperature sensor, the temperature control host is connected to the semiconductor refrigerator, and the temperature sensor is further connected to the semiconductor refrigerator.
2. The rat dorsal spine skin fold window chamber model system according to claim 1, wherein the upper frame is provided with a window hole protruding towards one side surface, the detachable window comprises a fixing ring and a cover glass, the edge of the window hole is provided with a first groove and a second groove, the cover glass is arranged on one side of the cover glass close to the lower frame, the edge of the cover glass is fixedly matched with the first groove, the fixing ring is arranged in the window hole and on one side far away from the lower frame, the edge of the fixing ring is fixedly matched with the second groove, and the cover glass is attached to the fixing ring.
3. The rat dorsal spine skin fold window model system according to claim 2, wherein the fixing ring is a plastic ring, the ring is provided with a notch, and openings are arranged at two ends of the plastic ring close to the notch.
4. The rat dorsal spine skin fold window model system according to claim 1, wherein the upper frame surface is provided with a plurality of first bolt ports and a plurality of first suture ports; the surface of the lower frame is provided with a plurality of second bolt ports and a plurality of second suture ports; after the upper frame and the lower frame are jointed, the first bolt hole and the second bolt hole are mutually inosculated; after the upper frame and the lower frame are jointed, the first suture opening and the second suture opening are mutually inosculated.
5. The rat dorsal ridge skinfold window model system according to claim 1, wherein the temperature control host comprises a microcontroller, a main circuit board and an H-bridge circuit;
the microcontroller is connected to the main circuit board; the main circuit board is connected to the temperature sensor and the H-bridge circuit; the H-bridge circuit is also connected to the semiconductor refrigerator.
6. The mouse dorsal spine skin fold window chamber model system according to claim 5, wherein the temperature control host further comprises a power module, a physical button module and a display module;
the power module is connected to the main circuit board, the physical key module is connected to the main circuit board, and the display module is connected to the main circuit board.
7. The rat dorsal ridge skin fold window model system according to any one of claims 1 to 6, wherein the upper frame is a titanium alloy frame and the lower frame is a hard polymer material frame.
8. The rat dorsal spine skin fold window model system according to any one of claims 1 to 6, wherein the upper frame is provided with a first fixing bracket, the length of the first fixing bracket is larger than the side length of the upper frame, and the first fixing bracket is integrally formed with the upper frame; the lower frame is provided with a second fixed support, the length of the second fixed support is greater than the side length of the lower frame, and the second fixed support and the lower frame are integrally formed; the length of the first fixing bracket is the same as that of the second fixing bracket.
Priority Applications (1)
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115191401A (en) * | 2022-07-06 | 2022-10-18 | 云南中医药大学 | Composite infection model stent and use method thereof |
| CN115191401B (en) * | 2022-07-06 | 2024-02-09 | 云南中医药大学 | Composite infection model bracket and application method thereof |
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