CN216386898U - Novel liquid phase chromatographic column pre-derivatization device - Google Patents
Novel liquid phase chromatographic column pre-derivatization device Download PDFInfo
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- CN216386898U CN216386898U CN202122762851.8U CN202122762851U CN216386898U CN 216386898 U CN216386898 U CN 216386898U CN 202122762851 U CN202122762851 U CN 202122762851U CN 216386898 U CN216386898 U CN 216386898U
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Abstract
The embodiment of the utility model discloses a novel liquid chromatography pre-column derivatization device, which comprises a liquid containing tank, wherein the bottom of the liquid containing tank is provided with a liquid outlet; a pump body provided with a solution inlet; the liquid outlet is connected with the liquid outlet, the liquid inlet is connected with the liquid outlet, and the liquid outlet is connected with the liquid outlet; the liquid feeding pipe is also internally provided with a pressure detection module, and the pressure detection module is used for detecting the liquid pressure in the liquid feeding pipe. According to the novel liquid chromatography pre-column derivatization device provided by the utility model, the pressure detection module in the liquid sending pipe detects the liquid pressure in the liquid sending pipe and controls the closing and opening of the electromagnetic valve and the opening size of the valve in real time according to the pressure signal, so that the detection solution in the liquid containing tank is supplied as required, impurities or interference peaks are not easily introduced, and the stability of the derivatization process is ensured.
Description
Technical Field
The utility model relates to the technical field of chromatograph equipment, in particular to a novel liquid chromatographic pre-column derivatization device.
Background
The liquid chromatogram-mass spectrometer, LC-MS for short, is an instrument for combining liquid chromatogram and mass spectrum, and is also an effective instrument for separating and analyzing complex organic mixtures. The existing liquid chromatography usually needs to derivatize a substance to be detected before detection, so that the effects of improving the sensitivity of sample detection, improving the separation degree of a sample mixture, being suitable for further structure identification (such as mass spectrometry, infrared or nuclear magnetic resonance) and the like are achieved.
The derivatization of the analyte includes a pre-column derivatization method and a post-column derivatization method, wherein the pre-column derivatization method is widely used due to advantages of relatively freely selecting reaction conditions, having no limitation of reaction kinetics, and easily allowing the multi-step reaction. It also has the following technical drawbacks and disadvantages: the pre-column derivatization needs complex instrument and equipment, impurities or interference peaks are easily introduced or samples are lost due to the fact that the operating state and working parameters of a fluid machine are usually adjusted manually in the derivatization process, and potential safety hazards are easily caused in the actual derivatization heating process.
Therefore, there is a need in the art to invent a novel liquid chromatography pre-column derivatization device with stable derivatization process and high safety performance.
SUMMERY OF THE UTILITY MODEL
In order to overcome at least one defect in the prior art, the utility model provides a novel liquid chromatography pre-column derivatization device which has the characteristics of simple and reasonable instrument design, stable derivatization process and high safety performance.
The technical scheme adopted by the utility model for solving the problems is as follows:
a novel liquid chromatography pre-column derivatization device, comprising:
the bottom of the liquid containing tank is provided with a liquid outlet;
a pump body provided with a solution inlet;
the liquid outlet is connected with the liquid outlet, the liquid inlet is connected with the liquid outlet, and the liquid outlet is connected with the liquid outlet;
the liquid feeding pipe is also internally provided with a pressure detection module, and the pressure detection module is used for detecting the liquid pressure in the liquid feeding pipe.
Further, the pump body is arranged on the movable support, and the liquid containing tank and the pump body are both fixedly arranged on the movable support so as to form a modular structure.
Furthermore, the pump body still is equipped with compressed air inlet, compressed air inlet is used for to the pump body lets in air, and compressed air inlet's size is adjustable.
Further, the device also comprises a reactor, wherein the reactor is communicated with the pump body and is used for containing the detection solution conveyed by the pump body.
Further, the reactor is provided with a driving motor and a stirring cutter, the stirring cutter is arranged in the reactor, and an output shaft of the driving motor is connected with the stirring cutter and used for controlling the stirring cutter to rotate.
Further, still include injector and chromatographic column, the injector with the reactor communicates, the chromatographic column with the injector communicates.
Further, the device also comprises a detection instrument, and the detection instrument is communicated with the chromatographic column.
In summary, compared with the prior art, the novel liquid chromatography pre-column derivatization device provided by the utility model at least has the following technical effects:
1) according to the novel liquid chromatography pre-column derivatization device provided by the utility model, the pressure detection module in the liquid sending pipe detects the liquid pressure in the liquid sending pipe and controls the closing and opening of the electromagnetic valve and the opening size of the valve in real time according to the pressure signal so as to supply the detection solution in the liquid containing tank as required, impurities or interference peaks are not easily introduced, and the derivatization process is stable;
2) the novel liquid chromatography pre-column derivatization device provided by the utility model realizes automatic adjustment of the opening of the electromagnetic valve through the pressure signal in the liquid delivery pipe, does not need manual intervention in the whole working process, has high operation efficiency and obviously improves the safety performance.
Drawings
FIG. 1 is a schematic diagram of the structure of the novel liquid chromatography pre-column derivatization device of the present invention;
FIG. 2 is a schematic view of the structure of the liquid holding tank and the oil pump of the present invention;
wherein the reference numerals have the following meanings:
1. a liquid containing tank; 11. a liquid outlet; 2. a pump body; 21. a solution inlet; 22. a compressed air inlet; 3. an electromagnetic valve; 4. a liquid delivery pipe; 5. a movable support; 6. a reactor; 61. a drive motor; 7. a sample injector; 8. a chromatographic column; 9. and (5) detecting the instrument.
Detailed Description
For better understanding and implementation, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention.
In the description of the present invention, it should be noted that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the utility model herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the utility model.
Referring to fig. 1 and 2, according to an embodiment of the present invention, a novel liquid chromatography pre-column derivatization apparatus includes:
the liquid detection device comprises a liquid containing tank 1, wherein a liquid outlet 11 is formed in the bottom of the liquid containing tank 1 and used for discharging detection solution in the liquid containing tank 1.
The detection device comprises a pump body 2, wherein the pump body 2 is provided with a solution inlet 21 for introducing a detection solution discharged from the liquid tank 1 into the pump body 2.
The liquid feeding device comprises a liquid feeding pipe 4 and an electromagnetic valve 3, wherein two ends of the liquid feeding pipe 4 are respectively connected with the liquid outlet 11 and the solution inlet 21, and the electromagnetic valve 3 is arranged on the liquid feeding pipe 4. Wherein, the liquid sending pipe 4 is connected with the liquid outlet 11 and the solution inlet 21 so as to communicate the liquid containing tank 1 and the pump body 2, and the electromagnetic valve 3 is used for controlling the flow rate of the solution in the liquid sending pipe 4.
In the technical solution of this embodiment, a pressure detection module is further disposed in the liquid sending tube 4, and the pressure detection module is configured to detect a liquid pressure in the liquid sending tube 4. Specifically, the pressure detection module in the liquid sending tube 4 detects the liquid pressure in the liquid sending tube 4, so that the closing, opening and opening of the electromagnetic valve 3 are controlled in real time according to the pressure signal, the detected solution in the liquid containing tank 1 is supplied as required, impurities or interference peaks are not easily introduced, the sample loss is avoided, and the stability of the derivation process is improved. More specifically, because the automatic adjustment of the valve opening of the electromagnetic valve 3 is realized by detecting the pressure signal in the liquid sending pipe 4, the whole working process does not need manual intervention, the operation efficiency is high, and the safety performance is obviously improved.
Referring to fig. 2, in a preferred embodiment of this embodiment, the novel liquid chromatography pre-column derivatization apparatus further includes a movable support 5, and both the liquid tank 1 and the pump body 2 are fixedly disposed on the movable support 5, so that both are in a modular structure. Specifically, the liquid containing tank 1 and the pump body 2 are integrated together through the movable base 5, so that the liquid containing tank and the pump body are combined to form a supply part of the novel liquid chromatography pre-column derivatization device, and the liquid chromatography pre-column derivatization device can adapt to different types of reaction devices and detection devices, so that the derivatization device can be selectively connected according to actual use requirements.
In another preferred embodiment of this embodiment, as shown in fig. 2, the pump body 2 is further provided with a compressed air inlet 22, the compressed air inlet 22 is used for introducing air into the pump body 2, and the size of the compressed air inlet 22 is adjustable. In the preferred technical scheme, the compressed air inlet 22 is used for introducing air into the pump body 2, and the opening, closing and working pressure of the pump body 2 are controlled by controlling and adjusting the amount of the compressed air introduced into the pump body 2, so that the adjustment of the flow rate of the solution entering the pump body 2 is finally realized.
Referring to fig. 1, in another embodiment of the present invention, the novel liquid chromatography pre-column derivatization apparatus further comprises a reactor 6, wherein the reactor 6 is communicated with the pump body 2 and is used for containing the detection solution conveyed by the pump body 2. In the technical scheme of this embodiment, the reactor 6 is specifically a shell container structure, and its inside is equipped with accommodation space for the detection solution that the splendid attire pump body 2 was carried, and when detecting solution and reaching the predetermined liquid level, stops to continue to carry solution, begins the reaction.
Referring to fig. 1, in a preferred embodiment of the present invention, the reactor 6 is provided with a driving motor 61 and a stirring tool, the stirring tool is disposed inside the reactor 6, and an output shaft of the driving motor 61 is connected to the stirring tool for controlling the rotation of the stirring tool. Specifically, the driving motor 61 drives the stirring tool inside the reactor 6 to rotate through the output shaft thereof, so that the detection solution inside the reactor 6 is fully and uniformly mixed, and the derivatization efficiency is improved.
Referring to fig. 1, in a further embodiment of this embodiment, the novel liquid chromatography pre-column derivatization apparatus further includes a sample injector 7 and a chromatography column 8, wherein the sample injector 7 is in communication with the reactor 6, and the chromatography column 8 is in communication with the sample injector 7. In the further technical scheme, the sample injector 7 is communicated with the reactor 6 and the chromatographic column 8, so that after sample injection parameters are set and a detection sample after reaction in the reactor 6 is introduced, a quantitative sample solution can be sent into the chromatographic column 8. More specifically, the column 8 is disposed in an incubator vessel and performs a separation analysis of a liquid-phase detection solution.
Referring to fig. 1, in a further embodiment of this embodiment, the novel liquid chromatography pre-column derivatization apparatus further includes a detection device 9, and the detection device 9 is in communication with the chromatography column 8. Specifically, the detecting instrument 9 is disposed in the last step of the novel liquid chromatography column pre-derivatization apparatus, and plays a role in detecting a sample solution and finally obtaining an analysis result.
In summary, according to the novel liquid chromatography pre-column derivatization device provided by the utility model, the pressure detection module in the liquid sending pipe 4 detects the liquid pressure in the liquid sending pipe 4, and controls the closing, opening and valve opening of the electromagnetic valve 3 in real time according to the pressure signal, so that the detection solution in the liquid containing tank 1 is supplied as required, impurities or interference peaks are not easily introduced, and the derivatization process is stable. In addition, the opening degree of the electromagnetic valve 3 is automatically adjusted through the pressure signal in the liquid sending pipe 4, manual intervention is not needed in the whole working process, and the operation efficiency and the safety performance are high.
The technical means disclosed in the utility model scheme are not limited to the technical means disclosed in the above embodiments, but also include the technical scheme formed by any combination of the above technical features. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of the present invention, and such improvements and modifications are also considered to be within the scope of the present invention.
Claims (7)
1. A novel liquid chromatography pre-column derivatization device is characterized by comprising:
the bottom of the liquid containing tank is provided with a liquid outlet;
a pump body provided with a solution inlet;
the liquid outlet is connected with the liquid outlet, the liquid inlet is connected with the liquid outlet, and the liquid outlet is connected with the liquid outlet;
the liquid feeding pipe is also internally provided with a pressure detection module, and the pressure detection module is used for detecting the liquid pressure in the liquid feeding pipe.
2. The novel liquid chromatography pre-column derivatization device of claim 1, further comprising a movable support, wherein the liquid containing tank and the pump body are both fixedly arranged on the movable support, so that the liquid containing tank and the pump body are in a modular structure.
3. The novel liquid chromatography pre-column derivatization device according to claim 1, wherein the pump body is further provided with a compressed air inlet, the compressed air inlet is used for introducing air into the pump body, and the size of the compressed air inlet is adjustable.
4. The novel liquid chromatography pre-column derivatization device of claim 1, further comprising a reactor, wherein the reactor is communicated with the pump body and is used for containing the detection solution conveyed by the pump body.
5. The novel liquid chromatography column front derivatization device of claim 4, wherein the reactor is provided with a driving motor and a stirring cutter, the stirring cutter is arranged inside the reactor, and an output shaft of the driving motor is connected with the stirring cutter for controlling the stirring cutter to rotate.
6. The novel liquid chromatography pre-column derivatization device of claim 4, further comprising a sample injector in communication with the reactor and a column in communication with the sample injector.
7. The novel liquid chromatography pre-column derivatization device of claim 6, further comprising a detection instrument in communication with the chromatography column.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122762851.8U CN216386898U (en) | 2021-11-11 | 2021-11-11 | Novel liquid phase chromatographic column pre-derivatization device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122762851.8U CN216386898U (en) | 2021-11-11 | 2021-11-11 | Novel liquid phase chromatographic column pre-derivatization device |
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| Publication Number | Publication Date |
|---|---|
| CN216386898U true CN216386898U (en) | 2022-04-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122762851.8U Active CN216386898U (en) | 2021-11-11 | 2021-11-11 | Novel liquid phase chromatographic column pre-derivatization device |
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| CN (1) | CN216386898U (en) |
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2021
- 2021-11-11 CN CN202122762851.8U patent/CN216386898U/en active Active
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