CN216378251U - Islet cell culture device - Google Patents
Islet cell culture device Download PDFInfo
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- CN216378251U CN216378251U CN202122620261.1U CN202122620261U CN216378251U CN 216378251 U CN216378251 U CN 216378251U CN 202122620261 U CN202122620261 U CN 202122620261U CN 216378251 U CN216378251 U CN 216378251U
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Abstract
The utility model discloses an islet cell culture device, which sequentially comprises the following components from top to bottom: the gas circulation layer comprises a gas cavity for containing gas, and the gas cavity is provided with a gas inlet and a gas outlet; a cell culture layer separated from the gas flow-through layer by a gas-permeable membrane which is gas-permeable and liquid-impermeable; the cell culture layer comprises a liquid cavity for accommodating liquid, and a liquid outlet and a liquid inlet are arranged on the liquid cavity; and a cell cluster isolation net is arranged between the cell debris collection layer and the cell culture layer, and the damaged islet cell clusters and the cell debris in the cell culture layer enter the cell debris collection layer through the cell cluster isolation net. The utility model can facilitate the culture of islet cells outside the body.
Description
Technical Field
The utility model relates to a biological culture vessel, in particular to an islet cell culture device.
Background
The existing diabetes has high incidence rate, and cannot be radically cured by only using a medicament, and an islet transplantation technology provides a new idea for radically curing the diabetes. The requirement for a short period of in vitro culture of islets prior to transplantation to reduce immunogenicity while reducing exocrine gland contamination provides more time support for pre-transplant recipient preparation.
When culturing cells, carbon dioxide is introduced into the incubator to adjust the pH of the medium. However, the islet cells need to survive under high oxygen conditions, and the conventional cell culture conditions cannot meet the aerobic conditions of the islet mass, so that the survival of the islet mass is greatly influenced. The dead islet cell mass will break, which is detrimental to the pre-transplant islet cell status.
The current cell culture is usually carried out in cell culture flasks, cell culture dishes and cell culture plates, the culture microenvironment lacks oxygen supply, oxygen enters cells in a limited way by diffusion, the pancreatic islet oxygen partial pressure in pancreatic organs is 30mmHg, the larger the pancreatic islet mass radius is, the lower the oxygen partial pressure in the pancreatic islet mass is, and the cells are subjected to hypoxia death. At present, no culture container capable of providing proper oxygen partial pressure for the culture outside the islet cell group exists, and the broken islet cell mass and the intact islet cell mass cannot be separated.
SUMMERY OF THE UTILITY MODEL
The utility model mainly aims to provide an islet cell culture device which can increase oxygen partial pressure of islet cells after in vitro separation, improve the anoxic state in a cell mass, thereby increasing the time, density and cell activity of in vitro culture of the islet cells and removing broken islet cells.
The technical scheme adopted by the utility model is as follows:
the utility model provides an islet cell culture device includes from last to down in proper order:
the gas circulation layer comprises a gas cavity for containing gas, and the gas cavity is provided with a gas inlet and a gas outlet;
a cell culture layer separated from the gas flow-through layer by a gas-permeable membrane which is gas-permeable and liquid-impermeable; the cell culture layer comprises a liquid cavity for accommodating liquid, and a liquid outlet and a liquid inlet are arranged on the liquid cavity;
and a cell cluster isolation net is arranged between the liquid and cell debris collection layer and the cell culture layer, and the damaged islet cell clusters and the cell debris in the cell culture layer enter the cell debris collection layer through the cell cluster isolation net.
According to the technical scheme, the liquid discharge port is formed in the bottom of the cell debris collection layer.
According to the technical scheme, the liquid outlet with a knob cover is arranged at the bottom of the cell debris collection layer.
According to the technical scheme, the gas cavity is a cylindrical cavity.
According to the technical scheme, the liquid cavity is a cylindrical cavity.
According to the technical scheme, the bottom of the cell debris collection layer is funnel-shaped.
According to the technical scheme, the upper part of the cell debris collection layer is wide and the lower part of the cell debris collection layer is narrow.
According to the technical scheme, the gas inlet and the gas outlet are symmetrically arranged on two sides of the gas cavity.
According to the technical scheme, the liquid outlet and the liquid inlet are symmetrically arranged on two sides of the liquid cavity.
The utility model has the following beneficial effects: according to the utility model, the three-layer structure arranged up and down is arranged, so that the culture space can be saved, and the cost of islet cell culture can be reduced. The gas circulation layer on the upper layer can improve oxygen supply for cells, improve survival conditions of islet cell clusters and solve the problem of islet cell cluster hypoxia death. Meanwhile, the designed lower liquid layer and the cell debris collecting layer can separate the broken islet cell mass through natural sedimentation, so that the broken islet cell debris can be conveniently removed.
Drawings
The utility model will be further described with reference to the accompanying drawings and examples, in which:
FIG. 1 is a schematic diagram of a islet cell culture apparatus according to an embodiment of the present invention;
FIG. 2 is a schematic structural diagram of an islet cell culture device according to another embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the utility model and are not intended to limit the utility model.
As shown in FIG. 1, the islet cell culture device according to the embodiment of the present invention comprises an upper, middle and lower three-part structure.
The upper layer is a gas flow-through layer 10, which comprises a gas inlet 11, a gas outlet 22 and a gas cavity for accommodating gas, and oxygen and carbon dioxide with different proportions can be introduced to adjust the oxygen partial pressure and pH value of the culture solution. The gas inlet 11 and the gas outlet 22 may be symmetrically arranged on both sides of the gas cavity.
The upper layer and the middle layer are separated by a gas semipermeable membrane, the gas semipermeable membrane is permeable to liquid and can reduce the action of the pressure of the gas in the upper layer on the pressure and the air flow rate of the culture solution in the lower layer, and the gas in the upper layer can be dissolved in the culture solution.
The middle layer is a cell culture layer 20 which comprises a liquid outlet 21 and a liquid inlet 22, so that automatic liquid change can be realized, and rich nutrient supply of the culture solution is guaranteed. The liquid outlet 21 and the liquid inlet 22 may be symmetrically arranged on both sides of the liquid cavity.
A cell cluster isolation net is arranged between the middle layer and the lower layer, and damaged islet cell clusters and cell fragments can pass through and settle to the bottom, so that the clean environment of the culture layer is guaranteed, and the cell cluster self-purification is realized.
The lower layer is a liquid and cell debris collection layer 30, which may have openings in its bottom to allow for drainage of the collected material. The knob cover can be arranged on the opening, and the water can be discharged completely after the knob cover is opened.
The types of cells that can be cultured by the islet cell culture device of the embodiment of the utility model include: islet cell mass, stem cell-derived islet cell mass, and the like.
The capacity of the islet cell culture device according to the embodiment of the present invention can be referred to a commercially available culture bottle.
The whole device of the islet cell culture device provided by the embodiment of the utility model can be a cylinder, the gas cavity is a cylinder cavity, and the liquid cavity is also a cylinder cavity.
Further, the cell debris collecting layer may be arranged to have a wide top and a narrow bottom to facilitate the drainage of the cellular fluids, for example, the bottom may be funnel-shaped, and the outlet of the funnel may be used as a discharge port.
As shown in FIG. 2, a housing 40 may be further provided outside the islet cell culturing device according to the embodiment of the present invention.
In conclusion, the islet cell culture device of the utility model can improve oxygen supply for cells through the upper gas circulation layer 10, improve the state of islet cells in an oxygen-deficient environment in vitro, improve the survival condition of islet cell mass, and increase the culture density of islet cells in unit area, thereby improving the time of islet cell culture in vitro and solving the phenomenon of islet cell mass oxygen-deficient death. According to the utility model, the three-layer structure is arranged up and down, so that the culture space and raw materials can be saved, the culture cost of the islet cells can be reduced, and meanwhile, the designed lower-layer liquid and cell debris collection layer 30 can separate the broken islet cell mass through natural sedimentation, so that the broken islet cell debris can be conveniently removed.
It will be understood that modifications and variations can be made by persons skilled in the art in light of the above teachings and all such modifications and variations are intended to be included within the scope of the utility model as defined in the appended claims.
Claims (9)
1. The utility model provides an islet cell culture device which is characterized in that from last to including down in proper order:
the gas circulation layer comprises a gas cavity for containing gas, and the gas cavity is provided with a gas inlet and a gas outlet;
a cell culture layer separated from the gas flow-through layer by a gas-permeable membrane which is gas-permeable and liquid-impermeable; the cell culture layer comprises a liquid cavity for accommodating liquid, and a liquid outlet and a liquid inlet are arranged on the liquid cavity;
and a cell cluster isolation net is arranged between the liquid and cell debris collection layer and the cell culture layer, and the damaged islet cell clusters and the cell debris in the cell culture layer enter the cell debris collection layer through the cell cluster isolation net.
2. The islet cell culture device of claim 1, wherein a liquid outlet is provided at a bottom of the cell debris collection layer.
3. The islet cell culture device of claim 1, wherein the bottom of the cell debris collection layer is provided with a liquid outlet with a knob cover.
4. The islet cell culture device of claim 1, wherein the gas cavity is a cylindrical cavity.
5. The islet cell culture device of claim 1, wherein the fluid cavity is a cylindrical cavity.
6. The islet cell culture device of claim 1, wherein the bottom of the cell debris collection layer is funnel-shaped.
7. The islet cell culture device of claim 1, wherein the cell debris collection layer is wide at the top and narrow at the bottom.
8. The islet cell culture device of claim 1, wherein the gas inlet port and the gas outlet port are symmetrically disposed on opposite sides of the gas cavity.
9. The islet cell culture device of claim 1, wherein the liquid outlet and the liquid inlet are symmetrically disposed on opposite sides of the liquid cavity.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202122620261.1U CN216378251U (en) | 2021-10-29 | 2021-10-29 | Islet cell culture device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202122620261.1U CN216378251U (en) | 2021-10-29 | 2021-10-29 | Islet cell culture device |
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CN216378251U true CN216378251U (en) | 2022-04-26 |
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CN202122620261.1U Active CN216378251U (en) | 2021-10-29 | 2021-10-29 | Islet cell culture device |
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2021
- 2021-10-29 CN CN202122620261.1U patent/CN216378251U/en active Active
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